| thymidine-kinase in cytomegalovirus infected cells. | in human diploid fibroblast lep cells infected with ad169 strain of human cytomegalovirus (cmv) a sharp increase of cytosol thymidine kinase activity was observed. the properties of the cytosol enzymes from infected and non-infected cells were compared. no significant differences between the enzymes from infected and control cells were observed in substrate specificity, ph dependence, thermostability and relative electrophoretic mobility. human sera containing high titres of cmv complement-fixin ... | 1976 | 13768 |
| stimulation of cellular thymidine kinases by human cytomegalovirus. | thymidine kinase (tk) activity in wi-38 and mrc-5 human fibroblasts was analyzed by discontinuous polyacrylamide gel electrophoresis (disc-page) and discontinuous glycerol gradient electrophoresis (disc-gep) after subculture or human cytomegalovirus (hcmv) infection. two peaks of tk activity with different relative fraction-of-migration (r(f)) values were resolved by disc-page or disc-gep in extracts from log-phase and infected cells. growing wi-38 cells expressed a slowly migrating (r(f) = 0.14 ... | 1977 | 20515 |
| human cytomegalovirus stimulates host cell rna synthesis. | human cytomegalovirus infection of human fibroblast cells (wi-38) induced cellular rna synthesis. the rna synthesis in infected cultures preceded the synthesis of viral dna and progeny virus by approximately 24 h. rna species synthesized in infected cells included ribosomal 28s and 18s; and 4s transfer rna; all were markedly increased in comparison to uninfected cells. this induction of host cell rna synthesis was dependent upon a protein(s) that was synthesized during the early stages of infect ... | 1975 | 163357 |
| partial characterization of a soluble antigen preparation from cells infected with human cytomegalovirus: properties of antisera prepared to the antigen. | soluble antigen (sa) preparations were obtained from cell cultures infected with either the davis or ad169 strains of cytomegalovirus (cmv). fractionation of sa preparations through sephadex g-200 resulted in a molecular weight value ranging from 67,000 to 85,000. rate-zonal centrifugation produced an approximate value of 5.5s for the cmv antigenic material. antisera to sa prepared from either ad169- or davis-infected cells lacked neutralizing activity but produced specific fluorescence confined ... | 1975 | 164507 |
| demonstration of the oncogenic potential of herpes simplex viruses and human cytomegalovirus. | | 1975 | 169029 |
| [late stages in the replicative cycle of human cytomegaloviruses in diploid cells. ultrastructural study]. | an ultrastructural study of late stages of the replicative cycle of human cytomegalovirus (cmv) in human embryo fibroblasts cell cultures has been performed. nucleocapsids are observed only inside the intranuclear inclusion. they mature apparently by taking the envelope from the structural components of microvescicular systems reaching from the nuclear membrane the periphery of the nuclear inclusion. however, some capsids reach "naked" the cytoplasm and then take the envelope from cytoplasmic me ... | 1975 | 180913 |
| cytomegalovirus infection of human lung epithelial cells in vitro. | human lung epithelial cells were productively infected with human cytomegalovirus in vitro. infectious virus was released up to 8 weeks postinfection. the cells retained their morphological characteristics throughout the period of observation, while simultaneously bearing all the features typical of cytomegalovirus infection. | 1975 | 181345 |
| [various ways of acquiring the pericapsule at the nuclear membrane level by herpes simplex virus and human cytomegalovirus]. | | 1975 | 182189 |
| plaque reduction neutralization test for human cytomegalovirus based upon enhanced uptake of neutral red by virus-infected cells. | foci of cells infected with human cytomegalovirus were noted to stain more intensely than uninfected cells with neutral red, and this provided the basis for development of a plaque assay and plaque reduction neutralization test for cytomegalovirus. plaques demonstrable by neutral red staining could be counted at 8 days after infection; thus, results could be obtained earlier than for plaque assay systems based upon the viral cytopathic effect, a fewer manipulations were required for staining cel ... | 1976 | 182716 |
| structural proteins of human cytomegalovirus. | | 1976 | 183384 |
| comparative activity of immunofluorescent antibody and complement-fixing antibody in cytomegalovirus infection. | three different tests for detection of antibodies to human cytomegalovirus (cmv), complement fixing with antigen prepared by freeze-thaw disruption (cf-ft) or with antigen prepared by extraction with alkaline glycine buffer (cf-ge) and immunofluorescent staining (fa), were compared in renal transplant recipients and their healthy donors, fa and cf-ge tests yielded positive results at an identical and significantly higher frequency than cf-ft in both donors and recipients. cf-ge and fa performed ... | 1976 | 184108 |
| induction of murine p30 by superinfecting herpesviruses. | the interaction of endogenous type c viruses with superinfecting herpes simplex virus type 2 (hsv-2) was investigated in two murine cell lines. replication of hsv-2 was suboptimal in random-bred swiss/3t3a cells and, in initial experiments, infection with a low virus-to-cell ratio resulted in carrier cultures with enhanced murine leukemia virus (mulv) p30 expression. immunofluorescence tests with swiss/3t3a cells productively infected with hsv-2 also showed hsv-associated cytoplasmic antigens an ... | 1976 | 184296 |
| antisera to human cytomegalovirus produced in hamsters: reactivity in radioimmunoassay and other antibody assay systems. | hamsters immunized with human cytomegalovirus (cmv) concentrated and purified by polyethylene glycol precipitation and density gradient centrifugation produced antisera with high titers of specific viral antibody, and which showed no significant reactivity with human host cell components. the antisera had high titers of cmv antibody in complement fixation, indirect fluorescent-antibody (fa), and neutralization tests, but titers obtained by indirect radioimmunoassay (ria) were markedly higher. th ... | 1976 | 185151 |
| [cytomegalic virus infection in normal and disturbed immunity. clinical and animal experiment studies]. | virological and serological studies of cytomegalovirus infections revealed following results: 1. the distribution of cytomegalovirus infections in the area of munich can be compared with other european areas. 57% of adults have antibodies against the cytomegalovirus. 2. cytomegalovirus is an important agent initiating infectious liver diseases in childhood. 3. the virus is also an important factor for severe cerebral retardation. 4. studies in adult mice showed that a non-pathogenic strain of mo ... | 1976 | 186373 |
| latent infection of mouse cells with human cytomegalovirus. | infection of secondary mouse fibroblast cultures with human cytomegalovirus (hcmv) led to the production of viral antigens in the absence of detectable virus replication. antigen production was not dependent on viral dna synthesis since it was not inhibited by cytosine arabinoside. beginning at 15 days post-infection, viral-specific antigens were no longer observable in infected cultures, but could be induced by iododeoxyuridine (iudr) treatment. such cultures carry hcmv genetic information in a ... | 1976 | 187270 |
| complement-fixing antigen of human cytomegaloviruses. | the titer of complement-fixing (cf) antigen of human cytomegalovirus (cmv) strain c87 was not reduced by 10 cycles of freezing and thawing or by storage at 4 c for two months, although the virus was labile at 37 c and 100 c. the rapid increase in titers of cf antigen and plaque-forming units was seen from days 3 through 6 after infection; however, cf antigen was detected 24 hr after infection. the molecular weight of cf antigen was estimated by gel filtration to be greater than 1.5 x 10(7) dalto ... | 1977 | 190330 |
| evidence for early nuclear antigens in cytomegalovirus-infected cells. | human cytomegalovirus (cmv) induces nuclear antigens resembling the epstein-barr nuclear antigen (ebna) as early as 3 h after infection. these early antigens can be detected only with the anti-complement immunofluorescence staining (acif) technique. synthesis of these new antigens is not influenced by cytosine arabinoside (ara-c). | 1976 | 190342 |
| human cells transformed in vitro by human cytomegalovirus: tumorigenicity in athymic nude mice. | athymic nude mice were inoculated with human embryo lung cells transformed in vitro by human cytomegalovirus (cmv). of the inoculated animals, 62% developed tumors after an average latent period of 19 days. the tumors were composed of small, polygonal cells with large nulei and scanty cytoplasm embedded in an abundant collagenous matrix. the cells were poorly differentiated but may have been of epithelial origin. adjacent structures were rarely invaded. cmv-related intracellular and membrane ant ... | 1977 | 191621 |
| double arc structures in nuclei infected with human cytomegalovirus. | | 1976 | 193488 |
| human cytomegalovirus: a review of developments between 1970 and 1976. part ii. experimental developments. | over the last 6 years, our knowledge of the basic characteristics of human cytomegalovirus (hcmv) has been greatly enhanced, especially as concerns the microbiology of this virus. dna relatedness studies show that there is 80% homology between different strains, regardless of their origin. studies on the relationships between hcmv and host-cell metabolism reveal that it induces both a virus specific and a cellular dna polymerase, stimulates ribosomal rna, induces early protein synthesis and res ... | 1977 | 194635 |
| human cytomegalovirions and dense bodies: glycopeptide analysis and mechanism of cell rounding and polykaryocytosis. | human cytomegalovirions and dense bodies labeled with [14c]glucosamine were analyzed by means of sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis and autoradiography. the same glycopeptide composition was found in both cytomegalovirions and dense bodies. these consisted of at least 11 glycopeptides which ranged in molecular weight from 46,500 to over 170,000 daltons. addition of purified human cytomegalovirus (cmv) at high multiplicity to confluent monolayers of human fibroblasts p ... | 1977 | 200587 |
| factors affecting the interferon sensitivity of human cytomegalovirus. | several factors affected the interferon sensitivity of human cytomegalovirus in human foreskin fibroblast cultures. an inoculum of infected cells was up to 300-fold less sensitive than a cell-free inoculum of equivalent input multiplicity. a 10-fold increase in the dose of infectious units of either type of inoculum was associated with a 10-fold or greater decrease in interferon sensitivity. several aspects of the virus-cell interaction were examined and parameters indicative of cell infection w ... | 1978 | 202571 |
| blastogenic response of human lymphocytes to human cytomegalovirus. | a method was developed for measuring the blastogenic response of human lymphocytes to human cytomegalovirus (cmv). viral and control antigens were prepared by extracting disrupted infected and uninfected cell cultures with an alkaline buffer. lymphocytes from ten donors with complement-fixing (cf) antibody exhibited a blastogenic response, whereas cells from ten seronegative donors did not. a relationship between the stimulation index (si) and the results of neutralization (nt), indirect haemagg ... | 1977 | 203423 |
| antigenic relatedness of 17 strains of human cytomegalovirus. | a 10 minute kinetic neutralization test was used to assess antigenic relatedness among 17 strains of human cytomegalovirus. hyperimmune guinea pig sera exhibited extensive cross-reactivity. however, the data did suggest that there may be four antigenic groups represented in this collection of cmv strains. available epidemiologic data for these strains do not provide any discernible basis for this grouping. | 1978 | 206134 |
| intracellular forms of the parental human cytomegalovirus genome at early stages of the infective process. | | 1978 | 208251 |
| replication of herpesviruses in human cells transformed by cytomegalovirus. | the replication of human cytomegalovirus (cmv) and herpes simplex virus (hsv) was studied in three human embryo cell lines (cmv-mj-hel-i, cmv-mj-hel-2, and cmv-mj-hel-2,t-i) transformed in vitro by human cmv. growth studies revealed that these cells were completely resistant to infection by cmv strains adi69 and mj and partially resistant to hsv types i and 2. neither virus dna nor virus proteins were synthesized in the transformed cells infected with cmv ad169. the hsv production in cmv-transfo ... | 1978 | 211187 |
| human cytomegalovirus-induced chromatin factors responsible for changes in template activity and structure of infected cell chromatin. | | 1978 | 214942 |
| inhibition of a complete replication cycle of human cytomegalovirus in actinomycin pre-treated cells. | the study of human cytomegalovirus (hcmv) in cultures of human embryo lung fibroblasts, pre-treated with actinomycin d, has shown that under these conditions the virus infection does not proceed beyond the 'early' events of the virus replication cycle. in the same experimental conditions the growth of poliovirus type i, vaccinia virus and herpes simplex type i virus, was completely unaffected. these results suggest that the complete hcmv replication cycle requires some cellular function(s) betwe ... | 1979 | 217966 |
| chronically persistent infection with human cytomegalovirus in human lymphoblasts. | cells from a line of human lymphocytes originating from a leukemic patient were persistently infected with human cytomegalovirus. assays of infectious centers and fluorescent antigen staining indicated that 1%--10% of the cells were infected. it appears that persistent infection is due to an equilibrium between the release of virus by infected host cells and the growth of uninfected cells rather than to defective virus or temperature-sensitive mutants. | 1979 | 220333 |
| induction of host dna synthesis and dna polymerase by dna-negative temperature-sensitive mutants of human cytomegalovirus. | | 1979 | 220788 |
| characterization of the human cytomegalovirus-induced chromatin factor responsible for activation of host cell chromatin template. | | 1979 | 224579 |
| size of infectious dna from human and murine cytomegaloviruses. | viral dna was isolated from human and murine cytomegalovirus by equilibrium centrifugation in cesium chloride gradients. the size of the dna was measured relative to t4 dna by velocity sedimentation in neutral glycerol gradients, and fractions were assayed for infectious dna. infectious murine cytomegalovirus dna sedimented as a single peak with an estimated molecular weight of 136 x 10(6). infectious human cytomegalovirus dna was detected in two peaks with molecular weights of 130 x 10(6) and 1 ... | 1979 | 225527 |
| production of plasminogen activator by human and hamster cells infected with human cytomegalovirus. | plasminogen activator was produced by both human embryo fibroblasts (a permissive system) and hamster embryo fibroblasts (a nonpermissive system) after exposure to human cytomegalovirus. the level of this activator was measured by using plates coated with [125i]fibrin. the production of plasminogen activator was enhanced when the human cells were exposed to human cytomegalovirus previously irradiated with uv light (5,520 to 55,200 ergs/mm2). | 1979 | 225562 |
| inhibition of cytomegalovirus-stimulated human cell ornithine decarboxylase by alpha-difluoromethylornithine. | 1. the relationship between synthesis of putrescine, human cytomegalovirus dna synthesis, cell dna synthesis, and human cytomegalovirus replication has been studied. 2. stimulation of ornithine decarboxylase activity by shifting low serum-arrested whole human embryo cells to high serum medium is inhibited more than 99% by 2.5 mm dl-alpha-difluoromethylornithine. the addition of dl-alpha-difluoromethylornithine to human cells arrested in low serum and subsequently stimulated by the addition of fr ... | 1979 | 227464 |
| a vesicular stomatitis virus (cytomegalovirus) pseudotype and its use in neutralization tests. | infection with vesicular stomatitis virus (vsv) of human diploid cells preinfected with the ad-169 strain of human cytomegalovirus (cmv) resulted in the formation of a vsv (cmv) pseudotype. its formation was favored by increasing the bicarbonate content in doubly-infected cultures. the pseudotype was capable of infecting not only human but also rabbit cells. pseudotype particles formed after infection with the tl 17 mutant of vsv, which carries a thermolabile lesion in its neutralization antigen ... | 1979 | 227814 |
| dna of human cytomegalovirus: size heterogeneity and defectiveness resulting from serial undiluted passage. | the majority of dna molecules associated with plaque-purified, low-multiplicity-passaged human cytomegalovirus (towne strain) had a molecular weight of approximately 150 x 10(6) and a molecular complexity of approximately 140 x 10(6). serial high-multiplicity passage resulted in the production of defective cytomegalovirions. an accumulation of smaller dna molecules packaged into virions was directly correlated with a decrease in infectivity and an increase in the particle-to-pfu ratio. the major ... | 1979 | 228055 |
| inactivation of human cytomegalovirus by the chemical carcinogen 4-nitroquinoline 1-oxide. | the infectivity of cytomegalovirus (cmv), strain davis, was inactivated by 4-nitroquinoline 1-oxide (nqo). a series of survival curves indicates that the rate of inactivation was directly dependent on the concentration of nqo over a range of 5 to 200 microgram/ml. at concentrations of i microgram/ml or less, inactivation of virus stock was not observed and at concentrations in excess of 200 microgram/ml, the cellular toxicity of residual nqo prevented quantification of the relatively low survivi ... | 1979 | 230304 |
| age-related neurocytotropism of mouse cytomegalovirus in explanted trigeminal ganglions. | human cytomegalovirus (cmv) causes severe congenital neurologic disease; adult neural infection is associated with transient cranial nerve palsies. in our experimental model, mouse cmv (mcmv) infects neurons and schwann and satellite cells of cranial nerve ganglions. to study the fate of mcmv in nerve tissue, we explanted trigeminal ganglions from newborn, suckling, and weanling mice, 3 to 36 days after intracranial inoculation. negative explants were co-cultivated with mouse embryo tissue cultu ... | 1979 | 230739 |
| enzyme-linked protein a: an enzyme-linked immunosorbent assay reagent for detection of human immunoglobulin g and virus-specific antibody. | a general-purpose reagent capable of reacting with immunoglobulin g in a modified enzyme-linked immunosorbent assay technique was prepared by using protein a coupled with horseradish peroxidase. the reagent detected low levels (0.003 to 1.0 microgram/ml) of human immunoglobulin g and was also applied in an enzyme-linked immunosorbent assay for titration of antibody to human cytomegalovirus. the antibody titers to human cytomegalovirus determined by enzyme-linked immunosorbent assay and by comple ... | 1979 | 231048 |
| comparative study of herpes group virus-induced dna polymerases. | a comparative biochemical study of virus-induced dna polymerases was made among the herpes group viruses: namely, herpes simplex virus (hsv) type 1 and type 2, human cytomegalovirus (hcmv) and varicella-zoster virus (vzv). although these virus-induced enzymes shared some biochemical properties, they differed in several important aspects. all these virus-induced dna polymerases could efficiently use poly(dc) . oligo(dg)12--18 and poly(da) . oligo(dt)12--18 as template-primers. however, in phospho ... | 1979 | 231586 |
| progressive neurologic disease caused by human cytomegalovirus. | | 1979 | 233327 |
| sequence determination and genetic content of an 8.9-kb restriction fragment in the short unique region and the internal inverted repeat of marek's disease virus type 1 dna. | the dna sequence (8.9 kb) covering about 70% of the short unique region (us) and part of the short inverted repeat of the marek's disease virus type 1 ga strain was determined. computer analysis of the sequence showed the presence of nine potential open reading frames (orfs), consisting of more than 300 nucleotides in the us region. of these orfs, four were found to be homologous to us10 (minor virion protein), us3 (protein kinase), us2, and us6 (gd) in the us region of alpha-herpesvirus herpes ... | 1992 | 1282282 |
| a possible role for nonsense suppression in the synthesis of a human cytomegalovirus 58-kda virion protein. | a 1.6-kb late mrna originating from the hindiii r fragment of human cytomegalovirus (hcmv) encodes a 58-kda virion phosphoprotein. data presented support the hypothesis that this protein may be synthesized via the translational readthrough of an opal termination codon separating two open reading frames located in tandem. to our knowledge this is the first report of nonsense suppression as a means of regulating gene expression in hcmv. | 1992 | 1309277 |
| characterization of human cytomegalovirus ul84 early gene and identification of its putative protein product. | the dna sequence and transcription pattern of human cytomegalovirus early gene ul84 were analyzed. this gene was mapped within a 2.6-kb psti fragment located between 0.534 and 0.545 map unit of the large unique segment of the human cytomegalovirus genome, which is adjacent to the pp65 and pp71 genes. a 2.0-kb mrna was transcribed from this region in the same leftward direction as the mrnas of the pp65 and pp71 genes. the message was first detected at 2.5 h postinfection and reached a maximal lev ... | 1992 | 1309892 |
| a 10-base-pair element of the human immunodeficiency virus type 1 long terminal repeat (ltr) is an absolute requirement for transactivation by the human cytomegalovirus 72-kilodalton ie1 protein but can be compensated for by other ltr regions in transactivation by the 80-kilodalton ie2 protein. | transient gene expression studies have indicated that human cytomegalovirus (hcmv) specifically transactivates the human immunodeficiency virus (hiv) long terminal repeat (ltr). we show here, by a specific mutational analysis, that only the tata box region is obligatory for transactivation of the hiv-1 ltr by hcmv. similarly, this element is also sufficient for transactivation by either the hcmv 72-kda major immediate-early 1 (ie1) or 80-kda ie2 gene product independently. however, deletion of a ... | 1992 | 1310765 |
| a human cytomegalovirus glycoprotein complex designated gc-ii is a major heparin-binding component of the envelope. | the purposes of this study were to determine whether heparin would block human cytomegalovirus (hcmv) infection of skin fibroblast (sf) cells and to identify hcmv envelope glycoproteins which might have affinity for heparin. it was determined that soluble heparin in concentrations of 5 to 20 micrograms/ml was capable of blocking hcmv infection of sf cells. however, after virus had adsorbed to the sf cells, heparin lost its ability to block infection. it was also determined that treatment of sf c ... | 1992 | 1310777 |
| development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus dna in blood samples from immunocompromised patients. | the presence of human cytomegalovirus (hcmv) dna in blood was investigated by the polymerase chain reaction (pcr) in 293 blood samples from 86 immunocompromised patients. of the 86 patients, 23 underwent clinical and virologic follow-up for hcmv infection. in parallel, blood samples were examined for viremia and antigenemia. concordant results between pcr and assays for viremia and antigenemia were obtained on 124 positive and 110 negative samples, with an overall concordance of 79.8%, while 59 ... | 1992 | 1311338 |
| processing of human cytomegalovirus envelope glycoproteins in and egress of cytomegalovirus from human astrocytoma cells. | the synthesis of human cytomegalovirus (hcmv) envelope glycoproteins and the production of infectious hcmv in human astrocytoma and skin fibroblast (sf) cells were analysed. hcmv envelope glycoproteins synthesized in astrocytoma cells had lower mrs than the same glycoproteins synthesized in sf cells regardless of the strain of hcmv used, showing that the differences observed were due to differences in processing by the host cell and not the strain of hcmv used. hcmv envelope glycoproteins synthe ... | 1992 | 1311355 |
| incorporation of the carbocyclic analog of 2'-deoxyguanosine into the dna of herpes simplex virus and of hep-2 cells infected with herpes simplex virus. | the carbocyclic analog of 2'-deoxyguanosine (cdg) is active against herpes simplex virus (hsv), human cytomegalovirus, and human hepatitis-b virus. in order to understand the mechanism of action of this compound against hsv, we have evaluated (a) the incorporation of [3h]cdg into viral and host dna in hep-2 cells infected with hsv and (b) the interaction of the 5'-triphosphate of cdg (cdg-tp) with the hsv dna polymerase and human dna polymerases alpha, beta, and gamma (ec 2.7.7.7). incubation of ... | 1992 | 1311407 |
| an acidic region of the 89k murine cytomegalovirus immediate early protein interacts with dna. | the product of the ie1 gene, the regulatory immediate early protein pp89 of murine cytomegalovirus (mcmv), interacts with core histones, which can mediate the association of pp89 with dna. we report the capacity of pp89 to interact directly with dna in the absence of cellular proteins. after separation of proteins by sds-page, pp89 bound ds- and ssdna, with a preference for ssdna. binding to specific dna sequences in the mcmv genome was not detected. the dna-binding region of pp89 was located to ... | 1992 | 1312114 |
| the herpes simplex virus type 1 tegument protein vp22 is encoded by gene ul49. | vp22 is a major tegument protein of herpes simplex virus type 1 and is highly phosphorylated in the infected cell. indirect evidence exists to suggest that it is encoded by gene ul49, present in the bamhi f fragment of the genome. using the polymerase chain reaction we have cloned the ul49 open reading frame into a mammalian expression vector under the control of the human cytomegalovirus immediate early gene promoter. after transfection into cos-7 cells expression of the gene product was detect ... | 1992 | 1312128 |
| [detection of cytomegalovirus in urine specimens from renal transplant recipients using polymerase chain reaction]. | human cytomegalovirus (cmv) in this study, a polymerase chain reaction (pcr) assay was developed to detect human cytomegalovirus (cmv) from urine specimens taken from renal transplant patients and one congenital cmv infection. twenty eight urine specimens of 20 renal transplant patients were analyzed by the pcr. cmv infection was detected in 11 specimens (8 recipients). the incidence (40.0%) of pcr positive in the renal transplant patients was highest among the other conventional methods includi ... | 1992 | 1312182 |
| detection of human cytomegalovirus in peripheral mononuclear cells and urine samples using pcr. | samples of peripheral blood lymphocytes from 105 different blood donors were investigated for the presence of human cytomegalovirus (hcmv) dna using the polymerase chain reaction (pcr) with primers specific for the pst i w fragment (ie region). viral dna sequences were detected in 53 samples, a fifth of which had been previously serotyped as hcmv negative. in the latter cases, western blot analysis re-determined two out of three individuals that were resampled as seropositive. pcr could therefor ... | 1992 | 1312222 |
| cytomegalovirus detection in kidney transplants: results obtained from the polymerase chain reaction. | kidneys from twelve renal transplant recipients were examined prior to and after graft failure and explantation. the investigations included conventional light and electron microscopy and the analysis of human cytomegalovirus-related viral proteins and viral dna. nucleic acid hybridization studies were conducted on kidney explants by an in situ method using dna probes and by the polymerase chain reaction employing primers and probe for immediate early gene targets. in none of the cases did light ... | 1992 | 1312918 |
| comparison of sensitivities of three commercial mrc-5 cell lines grown in shell vials to cytomegalovirus and responses to enhancing agents. | mrc-5 cells grown in shell vials from three suppliers (whittaker bioproducts, bartels, and viromed) were evaluated for sensitivity to human cytomegalovirus. previous studies done in our laboratory showed that treatment of the cells with dexamethasone (dex) and dimethyl sulfoxide (dmso) or these agents plus calcium improved the sensitivity of cells from whittaker. in this study, we compared the response of the whittaker cells with those of the cells from bartels and viromed, both treated and untr ... | 1992 | 1313044 |
| variations in the cytomegalovirus major immediate-early gene found by direct genomic sequencing. | an assay to detect and sequence dna from human cytomegalovirus (hcmv) immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction and direct solid-phase sequencing of the amplified material. urine samples from 16 patients tested positive for hcmv dna in both a colorimetric assay for the detection of immobilized amplified nucleic acids and a standard polymerase chain reaction assay with agarose gel electrophoresis. ten urine samples from h ... | 1992 | 1315340 |
| [a case of fatal malabsorption syndrome caused by strongyloidiasis complicated with isosporiasis and human cytomegalovirus infection]. | this 54-year-old korean coal miner suffered from continuous watery diarrhea and weight loss after corticosteroid treatment (beta-methasone, 4 mg daily for 1 week) due to hip-bone fracture in january 1991. except for the short therapy of steroid, no other histories were contributory. the malabsorption syndrome was aggravated while the case was treated under the impression of amebiasis or intestinal tuberculosis. aids antibody test by eia was negative and quantitative analysis of serum immunoglobu ... | 1992 | 1315569 |
| comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia. | the main parameters of immunostaining techniques, i.e., the type of fixative, immunocytochemical reaction, and quality of monoclonal antibodies (mabs), for quantitation of human cytomegalovirus (hcmv) antigenemia in peripheral blood polymorphonuclear leukocytes (currently performed by the indirect immunofluorescence or immunoperoxidase reaction by using mabs to hcmv pp65) were investigated in order to optimize procedural steps and reagents. significantly better results (in terms of the number of ... | 1992 | 1316367 |
| [effectiveness of phosphonoformic acid in herpes infections]. | the antiviral efficacy of phosphonoformic acid (pfa) was examined on tissue cultures of the human embryonal lungs against the viruses herpes simplex type 1 and 2, herpes zoster, and the human cytomegalovirus using the method of the inhibition of the cytopathic effect and against herpes simplex type 1 and 2 on the tissue cultures of vero cells using the methods of the inhibition of plaque formation. pfa was demonstrated to inhibit the reproduction of the viruses under study in both tests on tissu ... | 1992 | 1316808 |
| increased sensitivity for detection of human cytomegalovirus in urine by removal of inhibitors for the polymerase chain reaction. | the presence of inhibitors in urine interferes with the enzymatic reaction of the polymerase chain reaction (pcr) for detection of human cytomegalovirus (hcmv). to remove inhibitors, hcmv virions in urine were precipitated with polyethylene glycol, or dna was extracted from urine by the use of glass powder and subjected to pcr followed by southern blot hybridization with alkaline phosphatase-linked oligonucleotide probes. these simple, rapid methods increased significantly the sensitivity of pcr ... | 1992 | 1317879 |
| the human cytomegalovirus 80-kilodalton but not the 72-kilodalton immediate-early protein transactivates heterologous promoters in a tata box-dependent mechanism and interacts directly with tfiid. | we have asked how the human cytomegalovirus major immediate-early 1 (ie1) and 2 (ie2) proteins act to transactivate heterologous cellular and viral promoters. here we show that transactivation of the human immunodeficiency virus long terminal repeat and the 70,000-molecular-weight heat shock protein (hsp70) promoter by ie1 is tata box independent and that the ie1 protein does not interact directly with the tata box-binding factor tfiid. conversely, transactivation of these promoters by ie2 is ta ... | 1992 | 1318414 |
| microneutralization as a reference method for selection of the cut-off of an enzyme-linked immunosorbent assay for detection of igg antibody to human cytomegalovirus. | in view of developing an enzyme-linked immunosorbent assay (elisa) for the determination of igg antibody to human cytomegalovirus, a rapid microneutralization (nt) assay was used to test five positive standard sera containing increasing amounts of specific antibody and a negative standard serum. the standard serum containing the minimal amount of detectable nt antibody was selected as a cut-off standard for the elisa test. following preliminary testing on previously characterized sera which gave ... | 1992 | 1318488 |
| new monoclonal antibodies for the detection of immediate early antigens of cytomegalovirus. | two new monoclonal antibodies, cie-1 and cie-2, were developed for the rapid detection of human cytomegalovirus (hcmv) infection. they were found to be reactive with immediate early protein of hcmv in the nuclei of infected fibroblasts, as early as 3 hours post-infection. by radioimmunoprecipitation, cie-1 was found to react with a protein with an apparent molecular weight of 70,000, whereas cie-2 precipitated 2 proteins of 70,000 and 72,000 daltons, respectively. both monoclonal antibodies reco ... | 1992 | 1319172 |
| human cytomegalovirus ul97 open reading frame encodes a protein that phosphorylates the antiviral nucleoside analogue ganciclovir. | human cytomegalovirus (hcmv, a betaherpes virus) is the cause of serious disease in immunologically compromised individuals, including those with acquired immunodeficiency syndrome. one of the compounds used in the chemotherapy of hcmv infections is the nucleoside analogue 9-(1,3-dihydroxy-2-propoxymethyl)-guanine (ganciclovir). the mechanism of action of this drug is dependent on the formation of the nucleoside triphosphate, which is a strong inhibitor of the viral dna polymerase. thymidine kin ... | 1992 | 1319559 |
| laboratory diagnosis of congenital human cytomegalovirus infection using polymerase chain reaction and shell vial culture. | congenital hcmv infection was diagnosed at the 22th week of gestation. the infection was suspected because hcmv igm was detected in a serum sample obtained from the woman's husband. hcmv infection was detected in the amniotic fluid by polymerase chain reaction, shell vial culture (immunoperoxidase assay) and conventional virus isolation. serologic testing in paired sera of the woman and in umbilical cord blood for specific igm and iga remained negative. as serological data (preconceptional hcmv ... | 1992 | 1322865 |
| a novel herpes simplex virus gene (ul49a) encodes a putative membrane protein with counterparts in other herpesviruses. | comparative analysis of dna sequences located between the coding regions of genes ul49 and ul50 of herpes simplex virus types 1 and 2 (hsv-1 and -2) has revealed a small open reading frame (orf) of 91 and 87 codons respectively with the characteristics of a genuine protein-coding region. the predicted protein products are clearly related and exhibit features of membrane-inserted proteins, with potential n-proximal signal peptides and c-proximal membrane anchor regions. counterparts are present i ... | 1992 | 1322965 |
| use of ganciclovir for cytomegalovirus infection. | ganciclovir (9-(1,3-dihydroxy-2-propoxymethyl) guanine, dhpg) is an acyclovir analog with excellent antiviral activity against human cytomegalovirus (cmv). clinically, cmv infection occurs in from 60 to 90% of all renal transplant recipients and it is responsible for significant patient morbidity and graft loss. the likelihood of infection is closely related to the cmv status of both donor and recipient, with the greatest risk arising in the combination of a seronegative patient receiving a sero ... | 1992 | 1323340 |
| a continuous sequence of more than 70 amino acids is essential for antibody binding to the dominant antigenic site of glycoprotein gp58 of human cytomegalovirus. | antigenic domain 1 (ad-1) on glycoprotein gp58 of human cytomegalovirus was characterized in detail, using mouse and human monoclonal antibodies as well as human convalescent sera. series of procaryotically expressed fusion proteins and synthetic peptides of various lengths were used as sources of antigen. binding of antibodies was found to depend on a continuous sequence of more than 70 amino acids between residues 552 and 635 of gp58. the fine specificities for sequences involved in antibody b ... | 1992 | 1323695 |
| failure to detect human cytomegalovirus dna in peripheral blood leukocytes of healthy blood donors by the polymerase chain reaction. | the transmission of cytomegalovirus (cmv) by blood transfusion may have a major effect on certain immunocompromised patients. to protect susceptible blood recipients from infection, it is advisable to use blood components from cmv-seronegative donors. however, serologic tests are not capable of indicating which blood component actually harbors infectious virus and can transfer it to the recipient. therefore, a sensitive method is needed for the detection of the virus itself. there have been thre ... | 1992 | 1325693 |
| the immediate early genes of human cytomegalovirus upregulate expression of the cellular genes myc and fos. | human cytomegalovirus (hcmv) is an important pathogen of the lung. we determined whether the hcmv immediate early genes (ie1 and ie2) can alter the regulation of the cellular immediate early genes (c-fos and c-myc). plasmid constructs containing the promoter-regulatory regions c-myc or c-fos upstream of the reporter gene, chloramphemicol acetyl transferase, were co-transfected into t cells (jurkat cells), monocytes/macrophages (thp-1 cells), or human fibroblast cells with plasmid constructs cont ... | 1992 | 1325808 |
| on the association of human beta 2 microglobulin with cell culture-grown human cytomegalovirus (hcmv). | we studied the production of human beta 2 microglobulin (beta 2m) in mock-infected or human cytomegalovirus (hcmv) infected human embryonic lung fibroblasts (hel) and the association of human beta 2m with hcmv virions. titration of beta 2m by two-step sandwich enzyme immunoassay revealed that hel released considerable amounts of human beta 2m into the culture medium and that the production of beta 2m was significantly enhanced by hcmv infection. the concentration of human beta 2m in the culture ... | 1992 | 1326812 |
| glycoprotein h of human cytomegalovirus (hcmv) forms a stable complex with the hcmv ul115 gene product. | the human cytomegalovirus (hcmv) ul75 gene product is the homologue of herpes simplex virus type 1 (hsv-1) glycoprotein h (gh), a virion glycoprotein that is essential for infectivity and which is conserved among members of the alpha-, beta- and gamma-herpesviruses. it has previously been shown that hsv-1 gh forms a stable complex with hsv-1 gl, the product of the ul1 gene, and the formation of this complex facilitates the cell surface expression of gh. none of the open reading frames within the ... | 1992 | 1328481 |
| the 72k ie1 and 80k ie2 proteins of human cytomegalovirus independently trans-activate the c-fos, c-myc and hsp70 promoters via basal promoter elements. | growth-regulating cellular genes or genes encoding proteins involved in cell cycle control are likely to be major targets of viral gene products in the establishment of a cellular state favourable for a permissive infection. we have examined whether infection of permissive fibroblasts with human cytomegalovirus (hcmv) results in trans-regulation of such cellular genes. here we have shown that the proto-oncogenes c-fos and c-myc are specifically induced during immediate early (ie) and early times ... | 1992 | 1328493 |
| e2f mediates dihydrofolate reductase promoter activation and multiprotein complex formation in human cytomegalovirus infection. | the adenovirus immediate-early protein e1a activates the adenovirus e2 promoter and several cellular gene promoters through transcription factor e2f. the immediate-early proteins of human cytomegalovirus (hcmv) can complement an e1a-deficient adenovirus mutant and activate the adenovirus e2 promoter. hcmv also has been shown to activate the adenovirus e2 promoter. on the basis of these findings, we have investigated whether hcmv can activate the promoter of the cellular dihydrofolate reductase ( ... | 1992 | 1328853 |
| point mutations in the dna polymerase gene of human cytomegalovirus that result in resistance to antiviral agents. | three independently isolated mutants of human cytomegalovirus strain ad169 were found to be resistant to ganciclovir at a 50% effective dose of 200 microm. phosphorylation of ganciclovir was reduced 10-fold in mutant-infected cells compared with ad169-infected cells. all three mutants were also determined to be resistant to the nucleotide analogs (s)-1-[(3-hydroxy-2- phosphonylmethoxy)propyl]adenine (hpmpa) and (s)-1-[(3-hydroxy-2-phosphonylmethoxy)propyl]cytosine (hpmpc) and hypersensitive to t ... | 1992 | 1331515 |
| strong trans activation of the human cytomegalovirus major immediate-early enhancer by p40tax of human t-cell leukemia virus type i via two repetitive tax-responsive sequence elements. | the immediate-early 1 and 2 gene locus of human cytomegalovirus (hcmv) that encodes trans-activator proteins with effects on both homologous and heterologous promoters is expressed under control of a complex enhancer/promoter regulatory region. this enhancer contains four types of repetitive sequence elements with 17, 18, 19, and 21 bp that bind cellular transcription factors. although the hcmv enhancer acts as a powerful stimulator of transcription in most cell types examined, human t cells do ... | 1992 | 1331524 |
| human herpesvirus 7 is a t-lymphotropic virus and is related to, but significantly different from, human herpesvirus 6 and human cytomegalovirus. | an independent strain (ji) of human herpesvirus 7 (hhv-7) was isolated from a patient with chronic fatigue syndrome (cfs). no significant association could be established by seroepidemiology between hhv-7 and cfs. hhv-7 is a t-lymphotropic virus, infecting cd4+ and cd8+ primary lymphocytes. hhv-7 can also infect sup-t1, an immature t-cell line, with variable success. southern blot analysis with dna probes scanning 58.8% of the human herpesvirus 6 (hhv-6) genome and hybridizing to all hhv-6 strai ... | 1992 | 1332051 |
| the procoagulant response of cytomegalovirus infected endothelial cells. | the report describes the effect of an in vitro infection of human umbilical vein endothelial cells with human cytomegalovirus (cmv). the parameters studied are cellular procoagulant activity, secretion of plasminogen activator inhibitor (pai-1) and urokinase-type plasminogen activator (u-pa), activation and internalization of factor x and merocyanine 540 staining. the infection does not result in an increase in pai-1 and u-pa secretion, but it brings about a procoagulant response, which is relat ... | 1992 | 1332211 |
| improved synthesis and biological evaluation of an acyclic thiosangivamycin active against human cytomegalovirus. | we previously described the synthesis and in vitro antiviral activity of an acyclic thiosangivamycin analog (gupta et al., 1989a). in order to extend these initial studies, a new, multi-gram synthesis of 4-amino-7-[(2-hydroxy- ethoxy)methyl]pyrrolo]2,3-d]pyrimidine-5-thiocarboxamide (compound 229) was achieved in 5 steps from the known 5-amino-2-bromo-3,4-dicyanopyrrole in good overall yield. in plaque reduction assays with hcmv, compound 229 had an ic50 of 7 microm; in yield reduction assays th ... | 1992 | 1332597 |
| monoclonal anti-idiotypes for the rapid detection of human cytomegalovirus. | a novel enzyme-linked immunosorbent assay (elisa) was developed for human cytomegalovirus (hcmv) utilizing a monoclonal anti-idiotype specific for cmvb1, an antibody to hcmv. samples of hcmv were measured by their inhibition of the binding of cmvb1 to anti-idiotype. the elisa detected hcmv in a concentration-dependent manner from 20 to 0.6 x 10(3) pfu/ml, with 50% inhibition at approx. 3 x 10(3) pfu/ml. these data demonstrate the potential of anti-idiotype antibodies as the basis of simple and r ... | 1992 | 1333473 |
| evaluation of neutralizing antibody titers against human cytomegalovirus in intravenous gamma globulin preparations. | | 1992 | 1334581 |
| anti-viral activity of human recombinant heparin-binding proteins hbnf and mk. | herpes simplex viruses bind to cell surface heparan sulfate proteoglycans, as a first step of viral infection. we report here that two recombinant heparin-binding proteins hbnf and mk inhibit infectivity of human herpes simplex viruses types 1 and 2 and human cytomegalovirus. carboxymethylated hbnf and mk, which retain affinity for heparin-sepharose, do not exhibit anti-viral activities. arguments are presented that anti-viral effects of hbnf and mk are due to the competition for the specific bi ... | 1992 | 1335242 |
| discordant estimates of heterologous promoter activity as determined by reporter gene mrna levels and enzyme activity. | in this study, the human cytomegalovirus (cmv) promoter fused to the lacz (beta-gal) reporter gene was transfected into neuroblastoma sk-n-be(2)-c cells, and phorbol ester-stimulated promoter activity assessed by both pcr quantitation of reporter gene mrna levels and enzyme activity. surprisingly, significant differences were observed in the induction profile of cmv promoter activity as judged by these two independent methods of analysis. for example, at 24 hrs post-transfection beta-gal activit ... | 1992 | 1335248 |
| activity of different antiviral drug combinations against human cytomegalovirus replication in vitro. | the effects of different antiviral drug combinations on the replication of various human cytomegalovirus (cmv) strains in human embryonic lung (hel) fibroblasts were evaluated. hpmpc combined with either ganciclovir, foscarnet or acyclovir showed additive to synergistic inhibition of cmv replication. combinations of zidovudine with hpmpc, ganciclovir, foscarnet or acyclovir also resulted in additive to synergistic inhibition of cmv replication. synergism tended to be higher for the clinical cmv ... | 1992 | 1337893 |
| expression of a cytomegalovirus ie-1-factor viii cdna hybrid gene in transgenic mice. | a construct containing the 5' end of the human cytomegalovirus major immediate early gene fused to the human coagulation factor viii cdna was used to produce transgenic mice. two out of five transgenic lines transcribed the construct. the expression was consistently seen in a limited number of tissues and was highest in muscle tissues. this is in contrast to the almost ubiquitous activity demonstrated in earlier studies with the ie-1 enhancer/promoter. human factor viii protein was detected immu ... | 1992 | 1338694 |
| establishment and functional characterization of human herpesvirus 6-specific cd4+ human t-cell clones. | in order to clarify the protective immune responses against a newly identified herpesvirus, human herpesvirus 6 (hhv-6), we established hhv-6-specific human t-cell clones and examined their functional properties. five cd3+cd4+cd8- t-cell clones, which proliferated in response to stimulation with two different strains of hhv-6 in the presence of autologous antigen-presenting cells but not with herpes simplex virus type 1 or human cytomegalovirus, were established from peripheral blood lymphocytes ... | 1992 | 1348547 |
| human cytomegalovirus in rejected kidney grafts; detection by polymerase chain reaction. | human cytomegalovirus (cmv) infections are frequently associated with graft rejection in the immunosuppressed patients following organ transplantation. thirty-four tissue samples from rejected kidneys and 18 samples from normal adult kidneys obtained from autopsies were investigated for the presence of cmv-dna by the polymerase chain reaction (pcr) and by immunohistochemistry. dna extracted from renal tissues after proteinase k digestion was specifically amplified in 32 cycles using primers whic ... | 1992 | 1355597 |
| the dominant linear neutralizing antibody-binding site of glycoprotein gp86 of human cytomegalovirus is strain specific. | bacterial fusion proteins, constructed from overlapping fragments of the open reading frame coding for gp86 of human cytomegalovirus (hcmv) strain ad169, were used to localize antigenic regions recognized by antibodies from human convalescent sera. a major domain for binding of conformation-independent antibodies was localized on fusion protein ap86, containing amino acids 15 to 142 of gp86. human antibodies, affinity purified on ap86, neutralized infectious virus in tissue culture. in addition, ... | 1992 | 1371164 |
| infection of hematopoietic progenitor cells by human cytomegalovirus. | the susceptibility of hematopoietic progenitor cells to infection by human cytomegalovirus (hcmv) was investigated using several strains of hcmv, including the recombinant strain rc256. rc256 is derived from the laboratory strain towne and contains the escherichia coli lacz gene coding for beta-galactosidase (beta-gal) regulated by an early hcmv promoter. expression of lacz allowed the detection of hcmv in individual hematopoietic cells. clonogeneic bone marrow (bm) progenitors, including cd34+ ... | 1992 | 1377049 |
| the amino terminus of human cytomegalovirus glycoprotein b contains epitopes that vary among strains. | we mapped three antigenic domains of continuous epitopes on human cytomegalovirus (cmv) glycoprotein b (gb) by reacting a panel of independently derived monoclonal antibodies with deletion mutants expressed transiently in cos-1 cells. one of these antigenic domains, dc2, maps in the last 75 amino acids of the carboxy terminus. these epitopes are conserved in strains towne and ad169, as well as in 19 clinical cmv isolates. elisas of dc2-reactive antibodies with a set of overlapping synthetic olig ... | 1992 | 1378884 |
| monoclonal antibody e-13 (m-810) to human cytomegalovirus recognizes an epitope encoded by exon 2 of the major immediate early gene. | monoclonal antibody (mab) e-13 to human cytomegalovirus is used widely for diagnostic and fundamental studies, and has been shown to be directed against an immediate early (ie) protein(s). to determine which viral antigen is detected by mab e-13, four subfragments from the open reading frame encoded by exons 2, 3 or 4 of ie-1 were cloned in the bacterial expression vector pros. the resulting fusion proteins contained amino acids 77 to 491 encoded by mainly exon 4, amino acids 25 to 78 encoded by ... | 1992 | 1383398 |
| correction of alpha-l-fucosidase deficiency in fucosidosis fibroblasts by retroviral vector-mediated gene transfer. | a full-length cdna clone encoding the lysosomal hydrolase alpha-l-fucosidase was cloned into two retroviral vectors, one using the human cytomegalovirus immediate-early promoter for expression, and the other, the retroviral long terminal repeat (ltr). high-titer amphotropic virus was produced for both constructs by infection of pa317 cells, and used to efficiently transduce the alpha-l-fucosidase gene into both human and canine fucosidosis fibroblasts. this resulted in correction of the alpha-l- ... | 1992 | 1525209 |
| biochemical and immunological properties of rat recombinant interleukin-2 and interleukin-4. | we have previously described the isolation and sequencing of cdna clones encoding rat interleukin-2 (il-2) and interleukin-4 (il-4). in the present study, we report the generation of stably transfected chinese hamster ovary (cho) cell lines which constitutively synthesize and secrete high levels of rat recombinant il-2 (ril-2) and il-4 (ril-4). the expression of the cytokine cdna sequences is driven by the human cytomegalovirus promoter/enhancer within the respective pee6. hcmv-gs vector constru ... | 1992 | 1551691 |
| merocyanine-sensitized photoinactivation of enveloped viruses. | a wide range of enveloped viruses, including human herpes simplex virus type 1, human cytomegalovirus, human t cell leukemia/lymphoma virus type i, human immunodeficiency virus type 1, sindbis virus, and friend erythroleukemia virus, are highly susceptible to merocyanine 540 (mc 540)-sensitized photoinactivation. by contrast, human pluripotent hematopoietic stem cells, red cells, factor viii, and von willebrand factor are much less sensitive. this suggests that mc 540 may be useful for the inact ... | 1992 | 1617187 |
| subcellular distribution of the major immediate early proteins of human cytomegalovirus changes during infection. | the 72k immediate early (ie) 1 protein of human cytomegalovirus and the 68k protein, also encoded by the ie1 gene, were detected by immunoelectron microscopy using monoclonal antibodies specific for the 68k or 72k proteins. early after infection, both proteins were localized to the nucleus, but with different localization patterns. late after infection, both of the proteins decreased markedly in the nucleus, in which nucleocapsids appeared. simultaneously, the 68k protein became diffusely distri ... | 1991 | 1646281 |
| characterization of a 52k protein of murine cytomegalovirus and its immunological cross-reactivity with the dna-binding protein icp36 of human cytomegalovirus. | we have developed a hybridoma, designated 25g11, which produced a monoclonal antibody (mab) reactive with a 52k protein of murine cytomegalovirus (mcmv). this mab, 25g11, was reactive with a protein band of 52k in mcmv-infected cell lysates and with a protein of 49k in human cmv (hcmv)-infected cell lysates as detected by immunoblot analysis. with purified mcmv virions, 25g11 gave a faintly immunoreactive band of 52k. however, no immunoreactive protein band was detected with purified hcmv virion ... | 1991 | 1646282 |
| antibodies against human cmv-induced "early" antigens detected by immunoblotting are not exclusively associated with active infection. | we investigated the correlation between the prevalence of antibodies against human cytomegalovirus (hcmv) "early" proteins and the state of infection. two hundred and twenty-eight hcmv-igg-positive (hcmv late-antigen elisa) sera, drawn from subjects with active and non-active hcmv infections, were tested for specific igg antibodies against hcmv "early" antigens by a western blotting micromethod. applying virus strain ad-169, we found antibodies to hcmv "early" antigens in 59% of all cases, irres ... | 1991 | 1647051 |
| sensitive non-isotopic dna hybridisation assay or immediate-early antigen detection for rapid identification of human cytomegalovirus in urine. | a sensitive non-radioactive dna hybridisation assay employing digoxigenin-labelled probes was compared with immediate-early antigen detection and conventional virus isolation for the identification of human cytomegalovirus (hcmv) in 249 urine samples. of 44 specimens yielding hcmv by virus isolation, more were positive by dna hybridisation (32; 73%) than by immediate-early antigen detection (25; 52%) (p = 0.05). the specificity of the hybridisation assay in 45 apparently falsely positive specime ... | 1991 | 1648575 |
| value of animal models to evaluate agents with potential activity against human cytomegalovirus. | | 1991 | 1648821 |
| ganciclovir-resistant human cytomegalovirus clinical isolates; resistance mechanisms and in vitro susceptibility to antiviral agents. | | 1991 | 1648824 |