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infection of guinea-pig embryo cells with varicella-zoster virus.varicella-zoster (vz) virus has been shown to replicate in guinea-pig embryo (gpe) cells. when cell-free virus inocula were used, no infectious virus was liberated from these cells, either by freezing-thawing or ultrasonic disruption. however, cell-free virus was separated from gpe cells inoculated with infected human embryonic lung (hel) cells.1975172949
should patients with zoster receive zoster immune globulin?zoster developed in 55 patients, and 15, generalized disease occurred. twelve of 14 patients with dissemination had prompt appearance of both complement-fixing (cf) antibody and fluorescent antibody against varicella-zoster (vs) membrane antigen (fama). the geometric mean titers of vz antibody from the first to the fifth and the sixth through the tenth days following onset of zoster were similar in patients with localized and generalized involvement. two patients with disseminated zoster had und ...1975171458
antibody titers by mixed agglutination to varicella-zoster, herpes simplex and vaccinia viruses in patients with multiple sclerosis.antibody titers to varicella-zoster (v-z), herpes simplex (hsv) and vaccinia viruses were determined in sera collected in buffalo, ny, from patients with multiple sclerosis (ms), patients with other diseases, and normal individuals. the mixed agglutination test employing cell cultures infected with one of the above viruses as a source of antigen was used to determine antibody titers. the geometric mean titer (gmt) for v-z in 59 ms cases was significantly higher than that observed in patients wit ...1975170625
letter: possbile relation between herpes simplex and zoster. 1975168491
[letter: isolation of zoster patients]. 1975168169
application of a live attenuated varicella vaccine to hospitalized children and its protective effect on spread of varicella infection.twenty-three hospitalized children with no history of varicella or no detectable complement fixing (cf) antibody, were vaccinated with a live attenuated varicella vaccine (oka strain) immediately after the occurrence of a case of varicella in a children's ward of hospital. these children suffered from the nephrotic syndrome, nephritis, purulent meningitis, hepatitis etc., and 12 of them were receiving steroid therapy. an antibody response was noticed in all the vaccinated children, with mild fev ...1975167708
ineffectiveness of subcutaneous cytosine arabinoside in localized herpes zoster.cytosine arabinoside (cytarabine) was evaluated in a randomized double-blind controlled study for the treatment of localized herpes zoster. cytarabine was administered subcutaneously in a dose of 50 mg/m-2 body surface area once daily for 4 days, always within 14 days of onset of the first symptom and usually within 7 days. thirty patients given cytarabine and 30 patients given placebo were well matched with respect to age, sex, and length and severity of presenting rash and pain as well as unde ...1975166586
partial characterization of a soluble antigen preparation from cells infected with human cytomegalovirus: properties of antisera prepared to the antigen.soluble antigen (sa) preparations were obtained from cell cultures infected with either the davis or ad169 strains of cytomegalovirus (cmv). fractionation of sa preparations through sephadex g-200 resulted in a molecular weight value ranging from 67,000 to 85,000. rate-zonal centrifugation produced an approximate value of 5.5s for the cmv antigenic material. antisera to sa prepared from either ad169- or davis-infected cells lacked neutralizing activity but produced specific fluorescence confined ...1975164507
elevated immunofluorescence antibody titers to several herpesviruses in burkitt's lymphoma patients: are high titers unique?antibody titers for viral capsid antigens of all four human herpesviruses were measured by immunofluorescence in the sera of 16 burkitt's lymphoma (bl) patients, 16 age-, sex-, and locality-matched controls, and 136 family members from the west nile district of uganda. among family members, titers greater than 1:4 were found in 98% for herpes simplex virus (hsv), 86% for varicella-zoster virus (vzv), 100% for cytomegalovirus (cmv), and 94% for epstein-barr (ebv). titers in patients averaged appr ...1975163324
testing the properties of an experimental batch of zoster immune gammaglobulin (zig).the methods of indirct haemagglutination (ih) and precipitation in gel (id) were employed to test the level of varicella-zoster (vz) antibodies in an experimental batch of zoster gammaglobulin (zig). the titre of indirect haemagglutinating antibodies in zig was about 64 times higher than in the ordinary batches of normal immunoglobulin and about 8 times higher in comparison with the level of the initial plasma pool. in the reaction of precipitation in gel, zig produced 5 to 6 zones. in compariso ...197993127
the demonstration of cell-associated immunity to viruses. in vitro lymphocyte responsiveness to varicella-zoster antigen.the demonstration of in vitro lymphocyte responsiveness to common pediatric viruses has previously been fraught with many technical and conceptual problems. based upon our prior experience in demonstrating cell-associated immunity to mumps, rubella and measles viruses we illustrate our methodology and conceptual framework by documenting in vitro lymphocyte responsiveness to the varicella-zoster virus, another ubiquitous virus of childhood. we discuss our approach to the problems of reactivity, s ...197662002
letter: contact infection from live varicella vaccine recipients. 197657366
prophylaxis of varicella in children with neoplastic disease: comparative results with zoster immune plasma and gamma globulin.the incidence and severity of varicella following a close family contact were evaluated in children with neoplastic diseases who received prophylaxis either with commerical gamma globulin or with zoster immune plasma, as compared to patients who did not receive any prophylaxis. in the untreated group, all 14 patients developed varicella, complicated by 1 case of encephalitis and 2 cases of fatal pneumonia. in the group of 17 patients who received 0.6-1.2 ml/kg body weight of gamma globulin, 16 d ...197546775
improved yields of cell-free varicella-zoster virus.systematic studies on the replication of varicella-zoster virus in infected human fetal diploid lung cells have defined more optimal conditions for infection and harvesting of cultures and have led to the production of cell-free virus preparations with infectivity titers of greater than or equal to 10(6) plaque-forming units per ml. the highest yields of cell-free virus were obtained by (i) sonic treatment of the cellular phase of cultures inoculated with trypsin-dispersed infected cells at rati ...1976184051
cell-mediated immunity to varicella-zoster virus demonstrated by viral inactivation with human leukocytes.cell-mediated immunity to varicella-zoster (v-z) virus in persons immune to varicella has been demonstrated, using a tissue culture technique. cell-mediated immunity was reflected by the ability of peripheral leukocytes (lymphocytes and monocytes) from human donors to inactivate v-z virus. leukocytes were stimulated by the addition of noninfectious v-z antigen to cultures newly infected with v-z virus. several days leter, the v-z virus in these cultures was titered. when leukocytes from donors i ...1976184040
necrotizing retinopathy with herpes zoster ophthalmicus: a light and electron microscopical study.a necrotizing retinopathy following a vesicular cutaneous eruption in the distribution of the right trigeminal nerve developed in a patient who had been receiving systemic corticosteroid therapy one week prior to the onset of herpes zoster ophthalmicus. seven weeks after the herpetic symptoms began, the patient died of pneumonia following an intracerebral hematoma. at postmortem examination, unexpected multiple focal and confluent lesions, which corresponded to areas of extensive retinal necrosi ...1976180930
adenine arabinoside for therapy of herpes zoster in immunosuppressed patients: preliminary results of a collaborative study.eighty-seven immunosuppressed patients (53 with localized and 34 with disseminated herpes zoster) from 10 institutions were enrolled in a controlled therapeutic trial of adenine arabinoside. a crossover design was employed; thus, 47 patients received drug for five days and then received placebo, and 40 were given the two substances in the opposite order. resolution of acute pain and the cutaneous lesions were graded during a 10-day observation period. during the initial five-day period, treated ...1976180198
a case of peripheral facial palsy following varicella. 197551052
acute facial palsy. some clinical and virological observations.a prospective clinical and virological study on 44 patients with acute, peripheral facial paralysis was carried out in consecutive cases during one year. in 9 cases varicella-zoster infections were serologically established. in 5 additional patients an associated varicella-zoster, or herpes simplex, infection was possible. of the 9 confirmed cases, 6 were clinically diagnosed as zoster oticus, whereas on clinical grounds, 3 were regarded as bell's palsy. no evidence was obtained of associated en ...1976179268
detection of antibodies to varicella zoster virus by radioimmunoassay and enzyme immunoassay techniques.an enzyme assay for the detection of antibodies to varicella-zoster membrane antigen (ipama) and a solid phase radioimmunoassay (ria) which utilizes infected cell lysates as antigen are described. the results have been compared with those obtained by the indirect fluorescent antibody to membrane antigen (ifama) technique. it was found that ipama was equal in sensitivity to ifama. no cross-reactivity was found with other herpes group viruses. the ipama appears to give objective results, is easily ...197882905
varicella-zoster immunoglobulins during varicella, latency, and zoster.antibodies to membrane antigens of cells infected by varicella-zoster virus were detected by immunofluorescence. rises in varicella-zoster iga, igg, and tigm were detected after both varicella and zoster. prompt antibody responses were observed in patients with generalized zoster as well as in those with localized zoster. a delayed response was found, moreover, in one patient who did not develop disseminated disease. antibody to varicella-zoster virus was detected in the sera of all three patien ...1975169308
diseases associated with herpesviruses.human herpesviruses have been associated with numerous diseases throughout history (table 3), but their ability to induce latent and recurrent infections, alongwith their oncogenic capabilities, is only beginning to be understood. accordingly, our ability to deal with the diseases induced by herpesviruses is severely limited by our lack of information concerning the basic processes of virus-host cell interactions and systemic host factors; especially immune factors that are involved in the disea ...1976179753
varicella-zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique.a new plaque assay for the quantitation of varicella-zoster virus and a plaque reduction neutralization test for the determination of neutralizing antibody titer have been developed using the indirect immunoperoxidase technique. as compared with the classical plaque assay using a solid overlay, the test gives earlier results since plaque counting can be performed on day 3 after the inoculation of cell cultures. in six patients with zoster infection, neutralizing antibody titers ranged from 1:20 ...1976186483
early diagnosis of virus-caused vesicular rashes by immunofluorescence on skin biopsies. i. varicella, zoster and herpes simplex.the use of immunofluorescence (if) for the rapid identification of varicella-zoster (v-z) and herpes simplex (hs) antigen in frozen sections of biopsies of early non-vesicular skin lesions was investigated. direct if, using fluorescein- isothiocyanate- conjugated immuoglobulin (fitc ig) of paired human anti-v-z sera and fitc-conjugated negative and positive anti-v-z-monkey ig, yielded specific fluorescence of virus antigen in all 14 varicella cases investigated and in 10/11 zoster cases. in cont ...1976178050
the pathogenesis of simian varicella virus in cynomolgus monkeys.the mlm herpesvirus is infectious for cynomolgus monkeys. the disease in this species, possibly modulated by preinoculation antibody resembles human varicella. virus has been recovered from blood during the early incubation period, and from liver, lymph nodes, kidney, bladder and urine during the eruptive period of infection. the major target organs were skin and liver; specific pathological changes developed in both. appropriate antibody responses, including those to herpesvirus varicellae foll ...1975174129
an improved plaque assay for varicella virus. 1976184765
chickenpox vaccine. 1976190324
complement-fixing reactivity of varicella-zoster virus subunit antigens with sera from homotypic infections and heterotypic herpes simplex virus infections.various subunit antigens of varicella-zoster (v-z) virus were examined for complement-fixing (cf) activity with sera from homotypic infections and from herpes simplex virus (hsv) infections in which a cf antibody titer rise was demonstrated with crude v-z antigen. the subunit antigens included nucleocapsids, envelopes, a soluble antigen produced from infected culture fluids by sucrose density gradient centrifugation, a soluble antigen produced by reducing the volume of clarified infected culture ...1977192677
treatment of varicella-zoster virus infections with adenine arabinoside.twenty-three patients with complicated varicella-zoster virus infections were treated with adenine arabinoside. of 14 patients with herpes zoster, 13 had malignancy treated with irradiation and cytotoxic agents or steroids. although the duration of active vesicle formation in these patients ranged from two to 14 days before therapy, no new lesions appeared after the fourth day of treatment with adenine arabinoside. zoster encephalitis developed in one patient on the third day of treatment, and s ...1975165242
the varicella-zoster virus in the etiology of severe congenital defects. a survey of eleven reported instances. 1975165028
clinical and pathogenetic studies of medical lake macaque virus infections in cynomolgus monkeys (simian varicella).the medical lake macaque (mlm) virus produced varicelliform eruptions in cynomolgus monkeys. not all experimentally infected monkeys developed overt disease; viremia was found, and specific antibodies were detected. specific lesions were found in skin, lymph nodes, and spleen. focal inflammatory lesions were present in liver, pancreas, and lung (after intratracheal instillation of virus). mlm virus was recovered from these and other organs. the temporal movements of mlm virus in and out of prima ...1977192806
mink lung cells: a non-primate cell line highly susceptible for varicella-zoster virus.mink lung cells (mvilu) are highly susceptible to varicella-zoster virus (vzv). the titres of cell-free vzv suspensions reached 1.0 x 10(7) p.f.u./ml at 3 days post-infection, with subsequent cell degeneration, if mvilu cells were infected with a multiplicity of infectious virus of 0.01 p.f.u./cell. in contrast, during the same period and under the same conditions the titres of cell-free vzv were 10(2) to 10(3) times lower when grown on human foreskin fibroblasts. a fast and reliable plaque assa ...1978212517
[prevention and therapy of viral diseases with special reference to interferon].human leukocyte interferon (hli) was used for treatment of human diploid fibroblasts before and after infection with vaccinia virus, herpes simplex virus type 1 (hsv 1), herpes simplex virus type 2 (hsv 2), and varicella zoster virus (vzv). vero cells were infected with medical lake macaque herpes virus (mlmv), and treated with hli in the same way. in all of these systems hli exhibited an antiviral effect when administered before infection, and this effect could be increased by additional hli tr ...1977192653
varicella and herpes zoster: comparisons in the old and young. 1977191332
2-substituted derivatives of 9-alpha-d-arabinofuranosyladenine and 9-alpha-d-arabinofuranosyl-8-azaadenine.2-substituted derivatives of 9-alpha-d-arabinofuranosyladenine were prepared via the fusion of tetra-o-acetyl-alpha-d-arabinofuranose with 2,6-dichloropurine followed by stepwise displacement of the chloro groups. a different approach, the reaction of 2,3,5-tri-o-benzoly-d-arabinofuranosyl bromide with 2,6-bis(methylthio)-8-azapurine in refluxing toluene in the presence of molecular sieve followed by stepwise reaction of the blocked nucleoside with methanolic ammonia and then other nucleophiles, ...1977191611
complement fixing antibody to varicella-zoster virus in different age groups and after zoster.the titer of complement fixing antibody to varicella-zoster virus was studied in sera from 215 normal persons living in padua and its province and from 39 zoster patients. about 74% of sera from normal persons aged 21-80 years had antibody titers larger than or equal to 1/4. lower titers were present in the age groups 1-20 years and 81-90 years. sera from zoster patients had high titers from the second week following the onset of the rash. they were still comparatively high until the fifth month ...1977193530
acute peripheral facial palsy. part of a cranial polyneuropathy?in 14 of 16 consecutive patients with acute peripheral facial palsy, one or more (up to four) other nerves were involved. the nerves affected in addition to the facial nerve were as follows: trigeminal (ten patients), vestibular (eight), cochlear (six), vagus (one), and upper cervical (five). virus was not isolated from any of the patients. a fourfold increase or decrease in complement-fixing antibody titers was present in eight patients (in four, varicella-zoster; in one, varicella-zoster and m ...1977200210
[methods for the rapid diagnosis of herpes simplex using the indirect hemagglutination reaction].a method for preparation of an erythrocyte immunoglobulin herpes preparation for the indirect hemagglutination test (iha) is described. a high specificity and sensitivity of this test and the preparation has been demonstrated. the results of the use of iha with this preparation for the detection of herpes simplex virus in specimens from patients are presented.1977201105
clinical and serologic testing of a live varicella vaccine and two-year follow-up for immunity of the vaccinated children.a live varicella vaccine derived from the oka strain was given on 181 children who had no history of varicella and were seronegative by complement fixation (cf) and neutralization (nt) tests; 125 children were hospitalized and 54 were receiving steroid therapy. overall, seroconversion was achieved in 85.1% of the children by the cf test and in 97.8% by the nt test. clinical reaction consisting of mild fever and rash appeared in only two children. one hundred seventy-nine of the vaccinated childr ...1977202916
varicella-zoster virus: isolation and propagation in human melanoma cells at 36 and 32 degrees c.cell lines derived from human malignant melanoma tumors are susceptible to infection with varicella-zoster virus (vzv). within 5 days after inoculation of vesicular fluid, cytopathic changes appeared in melanoma cell monolayer cultures that were incubated at either 36 or 32 degrees c. the vzv isolates at the two temperatures were serially propagated by passage of trypsin-dispersed infected cells. a plaque assay was developed utilizing melanoma cell monolayers overlaid with nutrient medium contai ...1978203532
[virus secretion in varicella]. 1979220476
quantitation of antibodies to varicella-zoster virus by immune adherence hemagglutination.immune adherence hemagglutination was compared with the complement fixation test as a means of measuring antibodies to varicella-zoster virus. analysis of acute- and convalescent-phase sera from patients infected with varicella-zoster or with herpes simplex virus showed the immune adherence hemagglutination test to be more sensitive than the complement fixation test, and greater cross-reactivity between the two viruses appeared to be associated with the increased sensitivity. the two assay metho ...1978203604
[viral immunofluorescent technic--virus]. 1977203735
hyporesponsiveness of lymphocytes to virus antigens in rheumatoid arthritis.the immune response of peripheral blood lymphocytes to measles, rubella, parainfluenza types 1, 2, and 3 rna virus antigens and to varicella-zoster and herpes virus type 1 dna virus antigens was evaluated in 14 patients with rheumatoid arthritis (ra) and 14 matched controls by assessing 3h-thymidine incorporation. the results demonstrated hyporesponsivenss of lymphocytes from ra patients to virus antigens and phytohemagglutinin (pha), which did not appear to be nonspecific because of a lack of c ...1978205223
evaluation of zoster immune plasma. treatment of cutaneous disseminated zoster in immunocompromised patients.zoster immune plasma (zip) was evaluated for treatment of cutaneous disseminated zoster in immunocompromised hosts. twenty patients were studied: 13 were enrolled in a double-blind protocol, five received zip under an open protocol, and two were observed without receiving a transfusion. in the double-blind study, eight patients actually received zip; five were given plasma lacking varicella-zoster virus antibodies (control plasma). the clinical course of zoster in the group given zip was the sam ...1978205687
ocular surface manifestations of the major viruses.the ocular surface is often the first site of involvement by viral infections. background information, clinical presentations, and current treatment of three major viruses--herpes simplex, varicella-zoster, and adenovirus, have been presented in detail in this chapter. the effects of adenoviruses are usually transient and may be regarded as a nuisance. infection with herpes simplex or varicella-zoster may lead to prolonged activity of the disease and life-long treatment with occasional loss of u ...1979222704
[herpesvirus antibodies in hodgkin's disease and some lympho-hematopoietic diseases in children and adolescents]. 1977194715
multiplicity-dependent replication of varicella-zoster virus in interferon-treated cells.the inhibitory effects of interferon (if) on the replication of varicella-zoster (vz) virus in human foreskin fibroblast (hff) cultures inoculated with infected cells or with cell-free virus were assayed by measuring (1) yields of infected cells, (2) plaques, (3) microfoci and (4) cytopathic effects. more if was needed to reduce yields of infected cells at high input multiplicities of challenge than at low input multiplicites, and still more if was needed to prevent cytopathology due to vz virus ...1977195010
[complement fixation antibodies against varicella zoster virus in mexico city]. 1976194343
a practical method for preparation of varicella-zoster immune globulin.outdated blood from blood banks in massachusetts was screened for complement-fixing antibody to varicella-zoster virus (vzv). approximately 15% of the plasma units had a titer greater than or equal to 16, and one-half of these had a titer of greater than or equal to 32. plasma units with titers of cf antibody to vzv of greater than or equal to 16 were pooled. this pool had a cf antibody titer of 32 and a titer of fluorescent antibody of 64. varicella-zoster immune globulin was prepared from the ...1978207788
cell-mediated immunity to varicella zoster virus in children being treated for cancer.stimulation of lymphocytes by varicella-zoster virus (vzv) antigen was measured in vitro for 37 healthy adults and children and for 35 children who developed herpes zoster or varicella while receiving anticancer therapy. for 35 of the control group the history of varicella infection correlated with the in vitro response of lymphocytes to vzv antigen. a specific lymphocyte response was observed following 32 of the 37 episodes of vzv infection in the 35 children with malignancy. re-evaluation 7--1 ...1978208747
varicella-zoster viremia. 1978207842
[rapid diagnosis of infections caused by the chickenpox-herpes zoster viruses].a diagnostic preparation for the indirect hemagglutination test (iha) was obtained by sensitization of fixed tannin-treated sheep erythrocytes with igg-fractions from human herpes zoster convalescent sera. a high specificity of the preparation was established in comparative experiments. it could detect the varicella-herpes-zoster virus antigen in 100% of specimens from patients. the iha test with this erythrocyte diagnostic preparation is simple, economic, gives the results within 1-1 1/2 hours ...1978208309
[varicella vaccine]. 1978211282
summary of a workshop on new and useful methods in viral diagnosis. 1978212491
promising new antiherpes agent being tested in humans. 1978212617
use of a live varicella vaccine to prevent the spread of varicella in handicapped or immunosuppressed children including mcls (muco-cutaneous lymphnode syndrome) patients in hospitals. 1977208503
comparison of wi-38, mrc-5, and imr-90 cell strains for isolation of viruses from clinical specimens.with the diminishing supply of the human fetal lung wi-38 cell strain, a replacement for viral isolation is needed. two candidates are the human fetal lung strains mrc-5 and imr-90. a comparison of wi-38, mrc-5, and imr-90 was performed to evaluate efficiency and speed of viral isolation, clarity of cytophatic effect, and ease of growing the cells. the inocula were clinical specimens rather than tissue culture-adapted isolates. frozen samples of 46 specimens that had previously yielded an isolat ...1978211143
attempts to recover varicella virus from ganglia. 1977215073
antibody to varicella zoster virus in low-birth-weight newborn infants. 1978211219
varicella-zoster virus vaccine. 1977197280
[pathogenesis and topography of herpes zoster in childhood and in adult age]. 1977197719
varicella-zoster virus immunizes patas monkeys against simian varicella-like disease.to define further the antigenic relationship between human varicella-zoster virus and herpesviruses which produce varicella-like disease in certain simian species, patas monkeys were inoculated with varicella-zoster virus and then challenged with delta herpesvirus, which uniformly produces severe, clinically apparent disease in susceptible animals. protection against delta herpesvirus was conferred both by hyperimmunization with varicella-zoster virus and by a single immunization with a cell-fre ...1979215708
[clinical immunological characteristics of herpes zoster].changes of antibody formation of 96 patients with various clinical forms of herpes zoster were traced by indirect hemagglutination inhibition test. four variants of the immunological response were distinguished; clinical characteristics of each of these groups is presented. the initial antibody level failed to determine the severity of the course of the disease in herpes zoster.1978218405
simultaneous infection with multiple herpesviruses. 1979218450
csf viral antibodies. evaluation in amyotrophic lateral sclerosis and late-onset postpoliomyelitis progressive muscular atrophy.serum and csf from 48 patients with amyotrophic lateral sclerosis and six patients with late-onset postpoliomyelitis progressive muscular atrophy were investigated for the presence of antibody to poliovirus types 1, 2, and 3, coxsackie viruses b3 and b4, influenza a, measles, rubella, mumps, herpes simplex types 1 and 2, cytomegalovirus, varicella-zoster, and toxoplasma gondii. these results were compared with those from 53 control patients with neuromuscular disease matched for age, sex, race, ...1979220938
selective inhibition of the replication of varicella-zoster virus by 5-halogenated analogs of deoxycytidine. 1977212983
bell's palsy and herpes simplex. 1978213158
[prognosis of prenatal varicella-zoster-infections in relation to their onset during pregnancy (author's transl)].7 children were observed, whose mothers had varicella-zoster-virus-infection. 3 of these children developed postnatal herpes zoster without varicella in their history; 1 child had varicella in the neonatal period. there are indications that primary infection with varicella-zoster-virus during the first four months of pregnancy may cause deformities in the child; the same during the last five days of pregnancy increases the risk of varicella with a complicated course in the newborn. independent o ...1977196188
lymphocyte transformation and interferon production as measures of cellular immunity in lymphoma patients.our studies of in vitro cellular immune responses to herpesviruses have shown that, before treatment, lymphoma patients have decreased transformation to varicella-zoster virus antigen compared to herpes simplex virus and cytomegalovirus antigens. interferon production to varicella-zoster virus and cytomegalovirus antigens is also decreased. during the first six months of treatment, decreased lymphocyte transformation and interferon production to all herpesviral antigens was observed. among patie ...1978221398
induction of thymidine kinase and dnase in varicella-zoster virus-infected cells and kinetic properties of the virus-induced thymidine kinase.thymidine kinase (tk), dna polymerase, and dnase activities were induced in human foreskin fibroblasts after varicella-zoster virus infection. the induced tk and dnase activities have electrophoretic mobilities different from the corresponding host enzymes. varicella-zoster virus-induced tk was purified and separated from the host enzyme by affinity column chromatography. this enzyme has been shown to have a broader substrate specificity with respect to either the phosphate donor or acceptor as ...1979228052
antibody to varicella-zoster virus-induced membrane antigen: immunoperoxidase assay with air-dried target cells.a technique using indirect immunoperoxidase antibody to membrane antigen (ipama) was developed for detection of igg antibody to varicella-zoster virus (vzv). the ipama technique utilizes glass slides with air-dried vzv-infected cells, which can be stored at -70 c and used for several months without loss of sensitivity. antibody titers measured by the ipama technique were comparable to those measured by the technique using indirect fluorescent antibody to membrane antigen (ifama) for sera obtaine ...1979229177
analysis of the dnas from seven varicella-zoster virus isolates.the 32p-labeled dnas from seven different clinical isolates of human varicella-zoster virus (vzv) were independently digested with five site-specific restriction endonucleases, ecori, hindiii, smai, bamhi, and avai. the digestion products were analyzed by electrophoresis on 0.5% agarose gels followed by autoradiography of the dried gels. evaluation of the restriction enzyme cleavage patterns revealed small variations among the vzv dnas. the vzv dnas were also compared based on their buoyant dens ...1979229268
effect of ara-t on the replication of herpes simplex virus, varicella-zoster virus and cytomegalovirus.the thymidine analogue, 1-beta-arabinofuranosylthymine (ara-t), has previously been found to selectively inhibit herpes simplex virus (hsv) replication. at a relatively non-toxic concentration (50 microgram/ml), ara-t reduced hsv yields by a factor of 10,000-100,000. ara-t was also effective in inhibiting the replication of variecella-zoster virus (vzv) in vitro in human embryo fibroblasts, completely preventing vzv-specific cytopathic effects (cpe). ara-t reduced the cell-free virus and plaque- ...1978221418
comparative study of herpes group virus-induced dna polymerases.a comparative biochemical study of virus-induced dna polymerases was made among the herpes group viruses: namely, herpes simplex virus (hsv) type 1 and type 2, human cytomegalovirus (hcmv) and varicella-zoster virus (vzv). although these virus-induced enzymes shared some biochemical properties, they differed in several important aspects. all these virus-induced dna polymerases could efficiently use poly(dc) . oligo(dg)12--18 and poly(da) . oligo(dt)12--18 as template-primers. however, in phospho ...1979231586
zoster immune plasma prophylaxis of varicella: a follow-up report. 1979221631
the serological relationship of varicella-zoster virus to other primate herpesviruses.the serological relationship between viruses producing varicella-like exanthems in several subhuman primates, varicella-zoster virus (vzv) and herpes simplex viruses (hsv) types 1 and 2, was investigated. all the primate viruses were related to vzv and some to hsv also. these results show that there is a group of varicella viruses, comprising four distinct entities, some of which may be useful for the establishment of an animal model for varicella.1979232132
infection and persistence of varicella-zoster virus in lymphoblastoid raji cell line.infection of raji cells by varicella-zoster virus (vzv) resulted in permissive infection with establishment of a persistently infected lymphoblastoid cell line. vzv antigens of the membrane and nuclear type, as detected by the indirect immunofluorescence membrane antigen (ifama) and anticomplement immunofluorescence (acif) tests, were observed. minute amounts of infectious virus were detected by co-cultivation of vzv-infected raji cells (raji-vzv), with permissive human embryo fibroblasts (hef). ...1979232537
demonstration of anti-rubella antibody-secreting cells in rheumatoid arthritis patients.in the present study we describe a plaque-forming cell assay using erythrocytes coated with viral antigen, which detected anti-viral antibody-secreting cells against various viral antigens. these anti-viral antibody-secreting cell were studied in normal individuals with known viral infections and in rheumatoid arthritis patients. rubella anti-viral antibody-secreting cells were present after induction in the peripheral blood of eight out of ten patients. no plaques were seen before induction. sy ...1979472665
detection of herpesvirus dna in the large intestine of patients with ulcerative colitis and crohn's disease using the nested polymerase chain reaction.the prevalence of herpesvirus dna was examined in inflammatory bowel disease tissue. dna was extracted from resection and biopsy specimens of the large intestine from patients with ulcerative colitis (n = 21), patients with crohn's disease (n = 29), and patients with noninflammatory bowel disease (controls) (n = 21). the nested polymerase chain reaction was used to detect viral dna using primer pairs specific for either cytomegalovirus (cmv), herpes simplex virus 1 (hsv1), human herpesvirus 6 (h ...19921287131
[new viral vaccines, with special reference to chickenpox vaccine]. 1978215679
varicella vaccine in children with acute lymphoblastic leukemia and non-hodgkin lymphoma.to determine the safety and efficacy of varicella vaccine, 17 children with acute lymphoblastic leukemia (all) and 2 children with non-hodgkin lymphoma (nhl) receiving chemotherapy in remission were immunized with live attenuated varicella vaccine (oka strain). rash occurred in 7 children 7-53 days (median 24 days) after vaccination. there were 10-80 (median 20) erythematous vesicles and low-grade fever. all children required no specific treatment. there was no spread of varicella to their susce ...19921313688
idiopathic trigeminal sensory neuropathy.idiopathic trigeminal sensory neuropathy is a rare disorder characterized by transient sensory disturbances in the territory of one or more branches of the trigeminal nerve. acute and chronic forms of the disease have been recognized, but the boundaries between these groups are not clearly defined. six cases of idiopathic trigeminal sensory neuropathy are reported. all patients presented with sensory abnormalities limited to the territory of one trigeminal nerve, in the absence of other clinical ...19921315385
transcription from varicella-zoster virus gene 67 (glycoprotein iv).three transcripts map to the varicella-zoster virus (vzv) open reading frame (orf) 67, which encodes glycoprotein iv (gpiv). all of these transcripts are polyadenylated and are transcribed from left to right towards the genomic terminal short repeats. previous northern (rna) blot analyses suggested that the most abundant of these transcripts (1.65 kb) might code for gpiv. we performed s1 nuclease protection and primer extension assays and determined that the 5' terminus of the 1.65-kb transcript ...19921316476
[fatal varicella pneumonia in adults. report of two cases and review of literature (author's transl)].two mortal cases of varicella pneumonitis are described. the patients were respectively 41 and 34 years-old men of good health. they caught the disease from their children. the disease lasted one week, and death was due in both cases to respiratory failure. the autopsy confirmed massive lung involvement with varicella pneumonia, and also showed other organs involvement (liver, spleen, pancreas, lymph nodes, oesophagus). in both cases, human fibroblast cultures infected by material obtained from ...1979227015
biology and molecular aspects of herpes simplex and varicella-zoster virus infections.the herpes simplex and varicella-zoster viruses are members of the subfamily alpha herpesviruses with specific properties of the virion and with the capacity to establish latent infections in humans. the genome of each of these viruses has been determined with an estimate of the number of genes and proteins encoded. the biology and molecular events of the herpes simplex virus productive and latent infection have been detailed with the use of both in vitro and in vivo model systems. the neuron is ...19921317538
herpes zoster: pathogenesis and latency. 19921317598
the epidemiology of varicella-zoster virus infections: the influence of varicella on the prevalence of herpes zoster.this paper uses mathematical models and data analysis to examine the epidemiological implications of possible immunologically mediated links between patterns of varicella and herpes-zoster incidence in human communities. a review of previously published reports does not clarify whether or not there is a relationship between the incidence of varicella and the incidence of zoster. however, new analysis of data collected by the royal college of general practitioners provides indirect evidence for t ...19921318219
recurrent herpes zoster myelitis treated with human interferon alpha: a case report.recurrent herpes zoster myelitis is very rare. however, a case was recently observed in our hospital. a 43-year-old woman developed myelitis 2 weeks after development of shingles. her condition was improved by methylprednisolone. seven months later, she developed myelitis after development of shingles again. antibody against varicella-zoster (vzv), increased in the serum, but was negative in the cerebrospinal fluid. methylprednisolone was not sufficiently effective against this attack. the refra ...19921320320
restricted transcription of varicella-zoster virus in latently infected human trigeminal and thoracic ganglia.normal human trigeminal and thoracic ganglia latently infected with varicella-zoster virus (vzv) were identified by polymerase chain reaction (pcr). total rna was extracted from these ganglia and treated with dnase until ganglionic rna was free of vzv dna as determined by pcr. radiolabeled cdna synthesized by priming with random oligonucleotides was hybridized to southern blots containing recombinant clones that spanned greater than 95% of the vzv genome. the single region of the vzv genome dete ...19921320647
varicella vaccine: the american experience.live attenuated varicella vaccine is safe and effective in preventing chickenpox. the best immune responses occur in healthy children. leukemic children have a 50% incidence of mild-to-moderate adverse effects but have a high degree of protection once immune reactions to varicella-zoster virus (vzv) have developed. adult vaccinees have a lower degree of protection (70%) than children. vaccinees who develop breakthrough varicella usually have a modified infection. another significant advantage of ...19921320652
radioimmunoassay for antibodies in varicella-zoster virus serology.the method of ria for antibodies was employed with success in vz virus serology. the method is suitable for testing vz antibodies in the course of varicella or herpes zoster disease as well as for determining anamnestic titres. its advantages are stability of antigen, objective reading of results and applicability to testing large serum sets.1979231068
serological studies on the antigenic relationship between herpes simplex virus and varicella-zoster virus.if crossreacting antibodies between varicella-zoster virus (vzv) and herpes simplex virus (hsv) exist, one would expect more positive reactions with vzv in a group of hsv positive patients than in a group of hsv negative patients. this statement can only apply to a group of individuals where positive and negative reactions with respect to hsv and vzv are evenly distributed. such a distribution can only be found among children. therefore, the relationship between hsv-1 and vzv was the only one wh ...1979232073
risk factors for viral reactivation following bone marrow transplantation.the identification of risk factors for viral reactivation following transplantation is essential in order that antiviral prophylactic regimes may be allocated rationally. in the pretransplant period qualitative serological assessment of recipient and donor is informative with regard to the risk of post-transplant reactivation and disease. in addition, the quantitative level of herpes simplex virus (hsv) igg in the pretransplant recipient is predictive of post-transplant hsv excretion, although t ...19921322187
varicella vaccine expected to be ready by 1993. 19921322470
development of immunogenic recombinant oka varicella vaccine expressing hepatitis b virus surface antigen.recombinant oka varicella vaccine expressing hepatitis b virus (hbv) surface antigen (hbs) was constructed by inserting the hbs gene into the viral thymidine kinase (tk) gene and was examined for its immunogenicity in guinea-pigs. the hbs gene encoding 25 amino acids of pres2 and the whole of the s region was inserted into the tk gene of the cloned plasmid. the chimeric plasmid dna and oka varicella vaccine dna were cotransfected and recombinant virus was isolated after immunofluorescence screen ...19911646279
a novel herpes simplex virus gene (ul49a) encodes a putative membrane protein with counterparts in other herpesviruses.comparative analysis of dna sequences located between the coding regions of genes ul49 and ul50 of herpes simplex virus types 1 and 2 (hsv-1 and -2) has revealed a small open reading frame (orf) of 91 and 87 codons respectively with the characteristics of a genuine protein-coding region. the predicted protein products are clearly related and exhibit features of membrane-inserted proteins, with potential n-proximal signal peptides and c-proximal membrane anchor regions. counterparts are present i ...19921322965
colchicine treatment in the preparation of varicella-zoster virus inocula.two methods, ultrasonic and colchicine treatment, for preparing varicella-zoster virus (vzv) inocula were compared. ultrasonic treatment was unsuccessful in achieving the release of detectable, infectious, cell-free vzv from infected human foreskin fibroblast (sf) cells. sonication of infected sf cells resulted in losses of virus infectivity in direct correlation with losses in cell viability (r = 0.82). in the second method of generating vzv inocula, vzv-infected sf cells were treated for 15 mi ...19921313038
acyclovir treatment of varicella in otherwise healthy adolescents. the collaborative acyclovir varicella study group.to determine whether orally administered acyclovir is of therapeutic benefit for varicella in otherwise healthy adolescents, and to compare the severity of the disease in adolescents with that in younger children.19921313098
identification of the glycoprotein h gene of murine cytomegalovirus.partial sequencing of the hindiii c fragment of murine cytomegalovirus (mcmv) revealed an open reading frame of 2172 nucleotides in length encoding a 724 amino acid protein with a predicted mr of 80.4k. analysis of the predicted amino acid sequence revealed homology with glycoprotein h (gh) from a number of other herpesviruses. mcmv gh showed strongest amino acid identity with human (h) cmv and human herpesvirus 6 gh, and less identity with the gh protein sequences of epstein-barr virus, varicel ...19921321219
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