in vivo cloning of pseudomonas aeruginosa genes with mini-d3112 transposable bacteriophage.the transposition properties of the pseudomonas aeruginosa mutator bacteriophage d3112 were exploited to develop an in vivo cloning system. mini-d replicon derivatives of d3112 were constructed by incorporating broad host range plasmid replicons between short terminal d3112 sequences. these elements were made with small replication regions from the rk2, sa, and pvs1 plasmids and selectable genes for tetracycline, carbenicillin, kanamycin, and gentamicin resistance. some of the mini-d replicons a ...19892544563
[use of deletion mutants of plasmid rp4::d3112 for the genetic analysis of pseudomonas aeruginosa bacteriophage d3112].the hybrid plasmid rp4::d3112 becomes unstable in escherichia coli k-12 cells under certain growth conditions. the deletion mutants of this plasmid are formed at a high frequency. all the deletions selected have a specific feature: they start in the left end, at the point of joining of plasmid and phage dna, and remove different portions of the phage genome. the deletion mutants have been used for genetic mapping of d3112. we have localized the repressor gene ci (0-1.3 kb), 3 early genes (1.3-14 ...19836418616
[interaction of pseudomonas aeruginosa plasmids and mu-like phages: the suppression of the early stages of cell infection by phage d3112 in the presence of plasmid rpl11].the growth of mu-like, d3112, b39 and b3 bacteriophages of pseudomonas aeruginosa on bacterial strains containing r plasmids was studied. plasmids rpl11, rms148 and rms163 were shown to interfere with phage growth: 1) d3112 and b39 phages do not produce plaques on a lawn of pao1 (rms148) giving e.o.p. less than 10(-9); 2) rpl11 plasmid restricts phage d3112 growth (e.o.p. less than 10(-9), the growth of phage b3 being also restricted by this plasmid, though in considerably less extent; 3) phage ...19826807753
isolation and characterization of mutants defective in the cyanide-insensitive respiratory pathway of pseudomonas aeruginosa.the branched respiratory chain of pseudomonas aeruginosa contains at least two terminal oxidases which are active under normal physiological conditions. one of these, cytochrome co, is a cytochrome c oxidase which is completely inhibited by concentrations of the respiratory inhibitor potassium cyanide as low as 100 microm. the second oxidase, the cyanide-insensitive oxidase, is resistant to cyanide concentrations in excess of 1 mm as well as to sodium azide. in this work, we describe the isolati ...19957814333
[the effect of certain escherichia coli genes on the appearance of the tcs phenotype, conferred by plasmid rp4 with an integrated genome of the d3112 pseudomonas aeruginosa phage].the possibility of the sos system activation caused by introduction of a hybrid plasmid rp4::d3112 (where d3112 is a genome of the transposable phage of pseudomonas aeruginosa) into escherichia coli was examined. it has been shown previously that the presence of this plasmid confers to e. coli a so called tcs phenotype: e. coli (rp4::d3112) forms normal colonies and grows at 42 degrees c but does not divide and becomes filamentous at 30 degrees c, probably because of e. coli dna damages generate ...19938405972
characterization of the pseudomonas aeruginosa transposable bacteriophage d3112 a and b genes.the left end dna of mu-like transposable bacteriophage d3112 was sequenced from bp 2521 to bp 5483. two large open reading frames were identified: orf a (bp 2539-4611) and orf b (bp 4626-5378). orf a can encode a 690 amino acid, 78 kda protein which is 44.4% similar to mu transposase and orf b can encode a 250 amino acid, 27 kda protein, which is 46.4% similar to, though 62 amino acids shorter than, the mu b protein. the cloned d3112 a gene exhibited activity on a mini-d3112-containing plasmid i ...19958547306
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