reproduction of bacteriophage t6 and t7 in auxotrophic mutants of e. coli b. | | 1955 | 13327857 |
vitamin content of purified typhus rickettsiae, tobacco mosaic virus, and coliphage t6. | | 1956 | 13382844 |
recombinant f' factors from escherichia coli k-12 strains carrying recb or recc. | the frequency of genetic exchanges between f' factors and the bacterial chromosome was studied in recombination-deficient escherichia coli mutants under conditions in which the recombinant f' factors were immediately transferred to new hosts. in a series of double matings, f101-1 thr(+)leu(-) episomes were first transferred into each of four intermediate f(-)thr(-)leu(+) strains carrying various rec alleles. after the original f' donors were killed with phage t6, the f101-1 episomes were then tr ... | 1972 | 4553837 |
bacteriophage t4 early promoter regions. consensus sequences of promoters and ribosome-binding sites. | twenty-nine early promoters from bacteriophage t4 and 14 early promoters from bacteriophage t6 were isolated using vector m13hdl17, a promoterless derivative of m13mp8 carrying a linker sequence, the bacteriophage lambda-terminator tr1, and the lacz' gene including part of its ribosome-binding site. the consensus sequence for the t4 promoters is: (sequence; see text). ribosome-binding sites of t4 share the sequence: 5'...g.ggaga..aa.atgaa.a...3' the consensus sequence of the t4 early promoter re ... | 1989 | 2810355 |
stable albicidin resistance in escherichia coli involves an altered outer-membrane nucleoside uptake system. | albicidin blocked dna synthesis in intact cells of a pola- enda- escherichia coli strain, and in permeabilized cells supplied with all necessary precursor nucleotides, indicating a direct effect on prokaryote dna replication. replication of phages t4 and t7 was also blocked by albicidin in albicidin-sensitive (albs) but not in albicidin-resistant (albr) e. coli host-cells. all stable spontaneous albr mutants of e. coli simultaneously became resistant to phage t6. the locus determining albicidin ... | 1990 | 2191080 |
heterologous deoxyribonucleic acid uptake and complexing with cellular constituents in competent bacillus subtilis. | with competent cultures of bacillus subtilis the uptake of escherichia coli deoxyribonucleic acid (dna) is about 50% that for homologous dna. uptake of phage t6 dna, if any, is of the order of 7%, while nonglucosylated phage t6 (t6) dna is taken up almost as effectively as homologous dna. both t6 and t4 dna interfere only minimally with uptake of homologous dna; by contrast, t6 dna competes with homologous dna as effectively as the latter itself. these results indicate that the glucose residues ... | 1975 | 811646 |
fate of heterologous deoxyribonucleic acid in bacillus subtilis. | cscl density gradient fractionation of cell lysates was employed to follow the fate of escherichia coli, phage t6, and non-glucosylated phage t6 deoxyribonucleic acid (dna) after uptake by competent cells of bacillus subtilis 168 thy minus trp minus. shortly after uptake, most of the radioactive escherichia coli or non-glucosylated t6 dna was found in the denatured form; the remainder of the label was associated with recipient dna. incubation of the cells after dna uptake led to the disappearan ... | 1975 | 805125 |