| letter: lymphocyte agglutination in multiple sclerosis. | | 1975 | 52801 |
| persistent infection and viral carrier state of a human lymphoblastoid cell line infected with mumps virus before its transformation. | | 1977 | 202295 |
| structural polypeptides of mumps virus. | the structural polypeptides of egg grown mumps virus were analysed by sds-polyacrylamide-slab-gel electrophoresis. mumps virions contained eight major polypeptides with mol. wt. of 75, 73, 71, 61, 47, 44, 42 and 40 x 10(3). the 75 k and 61 k polypeptides were glycosylated. in virions treated with pronase and trypsin, the 75 k glycoprotein was removed more readily from the virus than the 61 k glycoprotein. the gradual removal of the 75 k glycoprotein was paralleled by a decrease of haemagglutinat ... | 1978 | 217949 |
| csf viral antibodies. evaluation in amyotrophic lateral sclerosis and late-onset postpoliomyelitis progressive muscular atrophy. | serum and csf from 48 patients with amyotrophic lateral sclerosis and six patients with late-onset postpoliomyelitis progressive muscular atrophy were investigated for the presence of antibody to poliovirus types 1, 2, and 3, coxsackie viruses b3 and b4, influenza a, measles, rubella, mumps, herpes simplex types 1 and 2, cytomegalovirus, varicella-zoster, and toxoplasma gondii. these results were compared with those from 53 control patients with neuromuscular disease matched for age, sex, race, ... | 1979 | 220938 |
| interactions between human lymphocytes and paramyxovirus-infected cells: adsorption and cytotoxicity. | the capacity of human lymphocytes to adhere to paramyxovirus-infected monolayers and their capacity to kill paramyxovirus-infected cells was investigated. a large fraction of human lymphocytes was found to adhere firmly to the paramyxovirus-infected monolayers. predsorption of lymphocytes on mumps virus-infected cells impaired their adsorption to a second cell monolayer of the same type. the cytotoxic activity of lymphocytes against mumps virus-infected cells was also reduced after predsorption ... | 1977 | 300397 |
| new tests for characterization of mumps virus antibodies: hemolysis inhibition, single radial immunodiffusion with immobilized virions, and mixed hemadsorption. | hemolysis inhibition (hli), single radial immunodiffusion (srid) with immobilized virions, and mixed hemadsorption tests were used for measuring antibodies against mumps virus. rabbit hyperimmune sera against mumps and early and late human convalescent sera were analyzed. all three tests identified antibodies against both hemagglutinin and the second major envelope component, hemolysin (fusion factor). the sensitivity of the hli test corresponded to that of the hemagglutination inhibition (hi) t ... | 1977 | 404314 |
| demonstration of anti-rubella antibody-secreting cells in rheumatoid arthritis patients. | in the present study we describe a plaque-forming cell assay using erythrocytes coated with viral antigen, which detected anti-viral antibody-secreting cells against various viral antigens. these anti-viral antibody-secreting cell were studied in normal individuals with known viral infections and in rheumatoid arthritis patients. rubella anti-viral antibody-secreting cells were present after induction in the peripheral blood of eight out of ten patients. no plaques were seen before induction. sy ... | 1979 | 472665 |
| studies on the use of mumps virus for treatment of human cancer. | purified mumps virus (urabe strain) was given mainly by intravenous injection to a total of 200 patients with cancer. the only adverse clinical reaction was transient mild fever in about half the patients. the beneficial clinical effects were as follows; decrease or disappearance of ascites and edema of the lower limbs at high rates (26/37 and 4/4, respectively), usually within a week after treatment: decrease or stoppage of cancerous bleeding in 30 of 35 patients: decrease or disappearance of p ... | 1978 | 749908 |
| screening for mumps immunity with the microtiter solid-phase radioimmunoassay. | a solid phase radioimmunoassay on microtiter plates was adapted for the estimation of mumps antibodies using commercially available complement-fixing mumps antigen. the sensitivity of the test is superior to that of hemagglutination inhibition and lies in the same range as the virus neutralization test performed on chick fibroblasts. the method is useful for screening large series of human sera, for instance in connection with vaccination programs. | 1976 | 977135 |
| [evaluation of cellular immunity in man using skin tests (methods)]. | | 1976 | 1023899 |
| suppression of delayed hypersensitivity in schistosome-infected patients by niridazole. | niridazole, an anti-parasitic drug, suppresses manifestations of delayed hypersensitivity and retards allograft rejection in laboratory animals. we investigated the immunosuppressive effects of the standard antihelminthic regimen of niridazole (25 mg/kg/day for seven days) in five patients with schistosomiasis. although 15-minute skin reactions to schistosomal antigens remained unchanged, niridazole reduced or ablated positive 48-hour skin reactions to tuberculin (ppd), mumps and schistosome ant ... | 1975 | 1124104 |
| human lymphocyte cytotoxicity against mumps virus-infected target cells. requirement for non-t cells. | subpopulations of human lymphocytes were tested for their capacity to kill mumps virus-infected target cells in a 51-chromium release asaay. using two different cell fractionation techniques, lymphocytes were fractionated into t cell-enriched (primarily t cells) and t cell-depleted (primarily b cells) subpopulations. filtration of lymphocytes through columns coated with human immunoglobulin and rabbit anti-human-immunoglobulin (ig-anti-ig) rendered the resulting t-cell preparation inactive as ef ... | 1975 | 1168673 |
| viruses in idiopathic inflammatory myopathies: absence of candidate viral genomes in muscle. | there is indirect evidence that various viruses have aetiological roles in the idiopathic inflammatory myopathies. by means of a sensitive and specific method based on the polymerase chain reaction (pcr), we sought direct evidence for the presence in affected muscle of nucleic acid sequences from coxsackie virus, mumps virus, encephalomyocarditis virus, adenovirus, human t-lymphotropic virus types i and ii, and human immunodeficiency virus. rna was extracted from muscle biopsy samples obtained f ... | 1992 | 1349938 |
| molecular cloning and sequence analysis of the mumps virus gene encoding the l protein and the trailer sequence. | we have cloned and determined the nucleotide sequences of the seventh gene of the miyahara strain of mumps virus (muv) encoding the l protein. the l gene is 6925 nucleotides in length and contains a single long open reading frame which is capable of coding for a protein of 2261 amino acids with a calculated molecular weight of 256,571 da. the deduced amino acid sequence of the l protein of muv showed significant homology with those of six other paramyxoviruses, human parainfluenza type 2 virus, ... | 1992 | 1585659 |
| evaluation of live attenuated varicella vaccine (oka-rit strain) and combined varicella and mmr vaccination in 13-17-month-old children. | the oka-rit strain of live attenuated varicella vaccine at dose levels 5300 pfu (high titre) and 2000 pfu (low titre) was tested in 13-17-month-old children; 50% of the children received the varicella vaccine alone, and the other 50% received it together with a measles-mumps-rubella (mmr) vaccine. the high titre and low titre varicella vaccines induced 96% and 92% seroconversion rates, respectively. following combined vaccination with mmr, the corresponding seroconversion rates for varicella wer ... | 1991 | 1661057 |
| restricted mumps virus infection of cells derived from normal human joint tissue. | mumps virus (muv) is known to be associated with acute arthritis and may also have a role in chronic inflammatory joint disease. the mechanism of induction of joint inflammation is not known but may be associated with direct invasion of joint tissue. to investigate the possibility of persistent intra-articular infection, the interaction of muv with primary cells from normal human joint tissue was examined. these mixed cultures of synovial membrane cells and chondrocytes were found to be semi-per ... | 1991 | 1993874 |
| sequence analysis of the hn gene of parainfluenza virus type 2. | a cdna library was constructed in lambda gt10 using mrna purified from cells infected with parainfluenza virus type 2 (piv2). virus-specific clones were identified by screening the library with 32p-labelled cdna probes made from randomly primed vrna. clones containing the haemagglutinin-neuraminidase (hn) gene were identified by sequence comparisons with known parainfluenza virus hn gene sequences. the largest hn clone isolated had a nucleic acid sequence of 2065 bp with a single long open readi ... | 1990 | 2161050 |
| sequence analysis of p gene of human parainfluenza type 2 virus: p and cysteine-rich proteins are translated by two mrnas that differ by two nontemplated g residues. | we cloned and sequenced the cdnas against genomic rna and mrna for phosphoprotein (p) of human parainfluenza type 2 virus (piv-2). cdna clone from genomic rna was 1439 nucleotides in length excluding poly(a) and was found to have two small open reading frames encoding proteins of 233 and 249 amino acids. two different mrna cdna clones were obtained; that is, one mrna contained a smaller reading frame coding 225 amino acids, v protein, and the other mrna contained a larger reading frame coding 39 ... | 1990 | 2162103 |
| viral antibodies in multiple sclerosis. a nationwide co-twin study. | serum viral antibody titers against 21 viruses were studied in 19 of 23 same-sex twin pairs with multiple sclerosis derived from the finnish twin cohort. thorough neurologic examinations showed two monozygotic pairs to be concordant, whereas all dizygotic pairs were discordant. special attention was given to measles, mumps, and rubella viruses, against which the antibody levels were determined with the complement fixation, hemagglutination inhibition, hemolysis-ingel, and enzyme immunoassay meth ... | 1990 | 2162658 |
| a sensitive elisa system for the rapid detection of virus specific igm antibodies in the cerebrospinal fluid. | the intrathecal production of igm antibodies to different viral antigens was measured by a modification of elisa that was both sensitive and specific. suitably diluted csf and homologous serum samples containing similar amount of igm were examined, and a comparison of the photometric signals permitted the detection of specific antibodies secreted from activated lymphocytes into the csf compartment during the course of viral infections of the central nervous system. polyvinyl chloride (pvc) micro ... | 1990 | 2162893 |
| mumps, enteroviruses, and human acute pancreatitis. | the presence of mumps virus and enterovirus rna was studied by in situ hybridization in 15 surgical biopsy specimens from patients with acute pancreatitis. 35s-labeled crna probes, detecting the 5' end of the poliovirus type 3, a 1.1-kb fragment of the polymerase gene region of coxsackievirus b3, and mumps virus mrna, encoding the nucleocapsid protein, were used. the controls consisted of mumps virus-infected and uninfected cultured cells, normal human and mouse pancreatic tissue, and mouse tiss ... | 1990 | 2171134 |
| t lymphocyte responses to coxsackie b4 and mumps virus. ii. immunoregulation by hla-dr3 and -dr4 associated restriction elements. | a major part of the t lymphocyte response to mumps and coxsackie b4 virus appears to be restricted by hla-dr associated restriction elements. this was further corroborated in inhibition experiments using monoclonal antibodies reactive with different hla class ii molecules. only antibodies reactive with dr molecules significantly inhibited the response. the frequencies of dr restricted antigen-reactive t lymphocytes (artl) to mumps and coxsackie b4 virus were then investigated, using a limiting d ... | 1985 | 2413585 |
| human mumps virus-specific cytotoxic t lymphocytes: quantitative analysis of hla restriction. | to obtain quantitative information about the use of hla antigens as restriction element by antiviral cytotoxic t lymphocytes (ctl), we have analyzed precursors of human mumps virus-specific ctl by limiting dilution. ctl generated by restimulation of peripheral blood t lymphocytes with autologous mumps virus (mv)-infected stimulator cells were restricted by autologous hla class i antigens, and derived from the t4-8+ population. they were specific for mv and did not lyse autologous target cells in ... | 1987 | 2435685 |
| human natural cytotoxic cells: activation by a measles virus-induced lymphocyte factor which increases the expression of the hnc-1a3 antigen. | when human lymphocytes were exposed to measles virus antigen or to sspe virus-infected cells, their cytotoxic activity against uninfected ma-160 cells was markedly enhanced. in contrast, mumps virus or herpes simplex virus-infected cells did not show such an enhancing effect and were rather inhibitory. sspe cells and measles virus antigen, but not mumps-virus-infected cells or mumps virus antigen, also induced a two-fold increase in the number of lymphocytes bearing the hnc-1a3 membrane antigen, ... | 1987 | 2445513 |
| paramyxovirus sv5 and multiple sclerosis. | multiple sclerosis is commonly associated with a local humoral immune response within the central nervous system. a hallmark of this intrathecal response is the presence of electrophoretically demonstrable oligoclonal bands of igg in the cerebrospinal fluid (csf) of up to 95% of patients. observations indicating that a major part of the csf igg in some patients may represent antibodies to sv5, a simian virus closely related to human parainfluenza type 2 virus, were recently reported by goswami e ... | 1989 | 2541340 |
| virus-specific polypeptides of human parainfluenza virus type 4 and their synthesis in infected cells. | we have studied the structural components of human parainfluenza virus type 4a (piv-4a) and identified some virus-specific polypeptides by immunoprecipitation with polyclonal and monoclonal antibodies followed by one- or two-dimensional sds-page. hn polypeptides existed as monomer, disulfide-linked dimer, and disulfide-linked larger oligomer in cells infected with piv-4a. interestingly, the nonreduced np, the nonreduced fusion, and the reduced f1 proteins migrated as doublets. two f1 polypeptide ... | 1989 | 2545032 |
| extensive antigenic diversity among human parainfluenza type 2 virus isolates and immunological relationships among paramyxoviruses revealed by monoclonal antibodies. | a panel of 128 monoclonal antibodies (mabs) directed against hemagglutinin-neuraminidase (hn), fusion (f), matrix (m), and polymerase (p) proteins, and nucleoprotein (np) of the toshiba strain of human parainfluenza type 2 virus (piv2) was prepared to examine the antigenic relationships among clinical isolates of piv2 and among paramyxoviruses by indirect enzyme-linked immunosorbent assays. the hn proteins of 18 clinical isolates of piv2 showed extensive antigenic diversity: 23 of 33 anti-hn mab ... | 1989 | 2545038 |
| immunological diagnosis in viral infections of the central nervous system: course of antibody titres against homo- and heterologous viruses. | in clinical cases suspected for viral encephalitis or meningoencephalitis, the estimation of virus-specific antibodies especially in liquor requires high sensitivity as well as specificity. with enzyme immunoassays the sensitivity in detecting antibodies has increased compared to e.g., complement fixation tests. this report concerns the determination of virus-specific antibodies with a commercial enzyme-linked immunosorbent assay (elisa) in paired liquor/serum samples of four patients with encep ... | 1989 | 2550755 |
| comparison between parainfluenza virus type 2 and simian virus 5: monoclonal antibodies reveal major antigenic differences. | marked differences in the apparent mrs of the hn, np and f proteins of simian virus 5 (sv5) and parainfluenza virus type 2 (pf-2) were revealed by sds-page. to examine the antigenic relationships between sv5, pf-2 and other paramyxoviruses, monoclonal antibodies (mabs) specific to pf-2 were isolated. these antibodies had specificities for the hn, np and p proteins and together with 54 mabs to sv5 were tested for their ability to react with sv5, pf-2, pf-3, mumps and measles virus proteins. most ... | 1988 | 2841416 |
| serology in facial paralysis caused by clinically presumed herpes zoster infection. | in some cases of peripheral facial palsy due to a clinically suspected varicella zoster virus (vzv) infection, the clinical diagnosis is not supported by serological tests. in a retrospective study, we examined the sera from 63 patients with clinical findings compatible with vzv infection: 57 had paired sera and 6 had single sera. in the paired-sera group, 18 cases were serologically negative initially by complement fixation (cf), while 9 of these cases were actually positive for vzv when cf was ... | 1988 | 2845904 |
| igg subclass distribution of antiviral antibodies in common variable immunodeficiency: effect of substitution therapy. | immunoglobulin g subclass titers to three herpesviruses (herpes simplex; hsv; cytomegalovirus, cmv; varicella zoster virus, vzv) were examined in patients with common variable immunodeficiency (cvi) before and after immunoglobulin substitution. like healthy controls, cvi patients expressed igg1 and igg3 to hsv and cmv, but only igg1 to vzv. individual titers varied as in healthy individuals, but as a mean, specific igg titers were lowered in proportion to the decrease of total igg. hsv and cmv i ... | 1988 | 2847890 |
| contribution to laboratory diagnosis of mumps and parainfluenza. | specific igm and igg antibodies to mumps virus (mv) were detected in sera of mumps-patients by elisa in agreement with the results obtained by indirect immunofluorescence (if). of given sera 37.5% contained igm reacting in indirect elisa also with the antigens of parainfluenza virus (piv) t3. in all patients with respiratory illness over 2 years of age, the significant increase of antibodies to piv in haemagglutination inhibition (hi) test was in good correlation with serum igm and igg antibody ... | 1988 | 2906222 |
| t lymphocyte responses to coxsackie b4 and mumps virus. i. influence of hla-dr restriction elements. | the proliferative t lymphocyte responses to coxsackie b4-, mumps- and varicella-zoster viral antigens were characterized. no significant difference in responsiveness was found between healthy individuals and patients with type 1 (insulin-dependent) diabetes mellitus. theophyllamine and verapamil decreased antigen-stimulated proliferation, whereas indomethacin in physiologic concentrations (1 microgram/ml) slightly increased proliferation. a major part of the response seemed to be restricted by h ... | 1985 | 2994251 |
| persistent synovial lymphocyte responses to mumps and adenovirus antigens. | a man in his 50s with rheumatoid arthritis showed maximal synovial lymphocyte reactivity to mumps antigen on 9 of 10 testings over a period of 6 years; peripheral blood lymphocytes showed no significant responses to mumps antigen in 5 testings over 5 years. a boy of 15 with recurrent arthritis of the right knee showed maximal synovial lymphocyte reactivity to adenovirus antigen. this reactivity was again present during a subsequent episode more than 3 years later; peripheral blood lymphocytes sh ... | 1988 | 3236305 |
| detection of postvaccination mumps virus antibody by neutralization test, enzyme-linked immunosorbent assay and sensitive hemagglutination inhibition test. | a group of 251 children aged 2-3 years given live attenuated mumps virus vaccine pavivac of czechoslovak production were tested for antiparotitis antibody levels in pre- and postvaccination sera by neutralization test (nt), enzyme-linked immunosorbent assay (elisa) and sensitive hemagglutination inhibition test, enhanced by heterologous antibody to human immunoglobulin g (e-hit). the prevaccination findings were as follows: positive elisa igg titres, neutralization antibodies and hemagglutinatio ... | 1987 | 3429853 |
| rubini, a new live attenuated mumps vaccine virus strain for human diploid cells. | a new, live attenuated mumps vaccine virus strain for human diploid cells has been developed at the swiss serum and vaccine institute, berne. the rubini virus was derived from a child of the same name possessing typical clinical signs and symptoms of mumps infection. attenuation of the wild virus was performed by isolation and serial passage in wi-38 human diploid cells, specific pathogen-free hens' eggs and mrc-5 human diploid cells. the attenuated virus has been examined in respect of identity ... | 1986 | 3556774 |
| development and duration of mumps fluorescent antibodies in various immunoglobulin fractions of human serum. | | 1970 | 4189108 |
| immune response of the human respiratory tract. ii. cell-mediated immunity in the lower respiratory tract to tuberculin and mumps and influenza viruses. | | 1973 | 4203077 |
| characteristics of secretory immune system in human middle ear: implications in otitis media. | | 1974 | 4205203 |
| virus susceptibility of marmoset kidney cell cultures. | | 1965 | 4284315 |
| viruses and disease: studies in a children's home. | | 1966 | 4287644 |
| virological studies on chromosome damage of hela cells induced by myxoviruses. | | 1966 | 4290662 |
| production of interferon by human leukocytes in vitro. | | 1966 | 4290664 |
| the removal of non-specific inhibitors from human and animal sera to mumps and para-influenza type i viruses by kaolin. | | 1966 | 4292182 |
| a serologic study on some respiratory viral and rickettsial infections in a rural community. | | 1965 | 4303321 |
| a respiratory virus study in great britain: review and evaluation. | | 1969 | 4313720 |
| maternal virus infection after the first trimester of pregnancy and status of offspring to 4 years of age in a predominantly negro population. | | 1970 | 4317248 |
| some properties of a new epithelial cell line of human origin. | | 1970 | 4317734 |
| viruses and chromosomes. | | 1970 | 4320571 |
| antibodies to various herpesviruses in sera from patients with sarcoidosis. | | 1971 | 4328945 |
| the effect of a carbobenzoxy tripeptide on the biological activities of measles virus. | | 1971 | 4332971 |
| workshop on viral infection and diabetes mellitus in man. | | 1972 | 4336861 |
| viral antibodies in the mano tribe of northern liberia. | | 1972 | 4344187 |
| a seroepidemiological study of epstein-barr virus and other viral antigens in sarcoidosis. | | 1973 | 4349694 |
| virus-specific immunoglobulins in multiple sclerosis. | | 1973 | 4353489 |
| [pathogenic organisms of infectious heart diseases]. | | 1973 | 4360233 |
| viral infections of the central nervous system. | | 1974 | 4362722 |
| virus antibodies in patients with multiple sclerosis. | | 1973 | 4365526 |
| treatment of human cancer with mumps virus. | | 1974 | 4611607 |
| meningoencephalitis and laboratory evidence of triple infection with california encephalitis virus, echovirus ii, and mumps. | | 1973 | 4697517 |
| [etiologic studies in the patients with central nervous system diseases hospitalized in rome during the period 1964-1970]. | | 1971 | 5153358 |
| replication of mumps virus in human leukocyte cultures. | duc-nguyen, huu (the children's hospital of philadelphia, philadelphia, pa.), and werner henle. replication of mumps virus in human leukocyte cultures. j. bacteriol. 92:258-265. 1966.-human peripheral leukocyte cultures maintained in the presence of phytohemagglutinin (pha) were found to support to some extent the replication of mumps virus. when such cultures were exposed, within 24 hr after their initiation, to a high input multiplicity of virus, successful infection, as determined by immunofl ... | 1966 | 5328751 |
| clinical studies of interferon. ii. recovery of interferon from cerebrospinal fluid of patients with aseptic meningitis. | | 1969 | 5347820 |
| [a retrospective study of 83 cases of children with aseptic meningitis]. | | 1970 | 5503255 |
| effect of puromycin and actinomycin d on a persistent mumps virus infection in vitro. | puromycin and actinomycin d were used to treat a line of human conjunctiva cells persistently infected with mumps virus (c-m cells) in order to determine where virus synthesis is inhibited. although 90% of the cells in c-m cultures are infected, little or no infectious virus is produced by most cells in a growing culture. adding puromycin to inhibit protein synthesis resulted in the production of infectious virus. thus, all the viral proteins needed for virus completion were made in the growing ... | 1969 | 5806982 |
| [experimental viral labyrinthitis--an immunohistochemical investigation of the cochlear lesion]. | pathogenesis of viral labyrinthitis is still poorly understood despite the dimension of clinical problem. this study was undertaken in order to elucidate the pathogenesis of viral labyrinthitis by the immunohistochemical investigations of infected cochleas. hvj (sendai virus) and mumps virus have been used to create an animal model of viral labyrinthitis. the cochleas of adult guinea pigs with intralabyrinthine inoculation of virus through the round window membrane and with intravascular injecti ... | 1986 | 3009291 |
| the molecular biology of the porcine paramyxovirus lpmv. | protein and genomic studies of a previously uncharacterized porcine paramyxovirus (designated lpmv) confirmed that it was a member of the paramyxovirus genus. the nucleotide sequences and deduced amino acids of the complete p-gene, m-gene, f-gene and hn-gene as well as the intergenic sequences have been determined. comparative sequence analysis of the m-gene of lpmv revealed the closest relationship of lpmv was to human mumps virus with a homology of 46% and 55% at the amino acid and nucleotide ... | 1992 | 1481359 |
| hydrocephalus and viral infections. | | 1975 | 173608 |
| chronic non-cytopathic infection of human continuous cell lines with mumps virus. | chronic infection with a vaccine strain of mumps virus (mv) was produced and studied in human cell cultures l-41 and hep-2. the establishment and course of the infection was not accompanied by cytopathic changes. among probable protective factors (mechanisms) interferon (ifn) was detected in l-41 culture and defective interfering particles in either cell culture. their role in the establishment and maintenance of chronic infection was not confirmed, however. | 1983 | 6138995 |
| [parotitis virus persistence in cell cultures of human origin]. | chronic infection of l-41 and hep-2 cells with mumps virus was studied. by their morphology and rate of growth the chronically infected cultures (cic) did not differ from the control cells. the cic produced the infectious virus in titres of 2.5-6.5 lg id50/ml. no virus could be detected after 48 passages in chronically infected l-41-mv cultures. virus-specific antigen could be detected by fluorescent antibody technique in the cytoplasm of most cic cells. in cic l-41-mv massive hemadsorption was ... | 1981 | 6170175 |
| leukocyte procoagulant activity in man: an in vitro correlate of delayed-type hypersensitivity. | human mononuclear leukocytes generate cell-bound procoagulant activity (lpca) after incubation with an antigen (mumps or tuberculin) to which the donor was previously sensitized. an inhibitor of coagulation appears to be liberated into the extracellular culture fluid during incubation of leukocytes with the sensitizing antigen. removal of this activity before measuring lpca resulted in a reliable test that correlated directly with delayed skin reactivity. the assay was particularly sensitive in ... | 1982 | 6172485 |
| rheumatoid factor in acute viral infections: interference with determination of igm, igg, and iga antibodies in an enzyme immunoassay. | igm rheumatoid factor (rf), an autoantibody to the fc fragment of igg, was determined by solid-phase enzyme immunoassay (eia). rf levels were significantly higher in patients with rubella virus infection than in patients with infections due to influenza virus, cytomegalovirus, respiratory syncytial virus, parainfluenza virus, adenovirus, mumps virus, or herpes simplex virus. to evaluate the role of rf in eia determinations of viral antibodies, igm rf from igm-igg cryoglobulin or control igm was ... | 1980 | 6251140 |
| comprehensive viral immunology of multiple sclerosis. iii. analysis of csf antibodies by radioimmunoassay. | the csf from 279 patients with multiple sclerosis (ms), probable ms, or controls was examined by radioimmunoassay (ria) for antibodies to measles, rubella, mumps, parainfluenza 1 (sendai) (strain 6/94), herpes simplex (hsv), varicella, and vaccinia viruses. significantly more patients with ms than noninflammatory control patients had antibody to measles, rubella and varicella viruses, of which antibody to measles was the most prevalent. the percentage of patients with ms with two or more csf ant ... | 1980 | 6252875 |
| viral diabetes mellitus in man and experimental animals. | accumulating evidence strongly suggests that viruses may play an etiologic role in some cases of diabetes mellitus. the importance of environmental factors in the pathogenesis of the disease is suggested by genetic and epidemiological observations. group b coxsackie viruses and mumps virus have been implicated, and the evidence suggests that several other agents also could play a role in the disease. in experimental animals, a picornavirus similar to the coxsackie viruses exhibits specific tropi ... | 1981 | 6257111 |
| epstein-barr virus antibodies in children with atopic disease. | the prevalence and titers of antibodies to human herpetoviruses as well as rubella and mumps viruses were determined in 98 children with atopic diseases and in 55 nonatopic control children. the only significant difference between the two groups was found as regards epstein-barr virus (ebv) antibodies, which were found in higher titers in atopic children of all ages. increased levels of ebv antibodies were found both in children with asthma and atopic dermatitis. the frequency of seropositivity ... | 1981 | 6260689 |
| viral antibodies in multiple sclerosis. | | 1982 | 6288620 |
| [a rapid method for the determination of virus-specific igm antibodies]. | a newly developed ion exchange chromatography is reported; it is a simple and quick method to separate a highly purified igm fraction from blood serum. the immunofluorescence test was used to demonstrate that these fractions contain only virus-specific igm antibodies, but no demonstrable igg antibodies, which can often block the specific igm reactivity by competitive inhibition. the specificity of the reaction was tested on sera from 145 persons: the igm test was always positive in clinically pr ... | 1982 | 6293973 |
| [patterns in the spread and serovirological diagnosis of serous meningitis]. | the epidemiological and clinico-etiological study of cases of acute serous meningitis with unknown etiology in children was carried out in a large industrial city at the period of a considerable morbidity rise caused by this infection. the maximum morbidity was registered among younger children under school age attending children's institutions. in 26 closed groups of children group morbidity was revealed (4 cases and more), in 5 such groups small local outbreaks were registered. the clinico-ins ... | 1982 | 6297190 |
| hemadsorption immunosorbent technique for determination of mumps immunoglobulin m antibody. | we used hemadsorption immunosorbent technique (hit) to detect mumps immunoglobulin m (igm) antibody. igm from human sera was adsorbed into anti-human igm-coated wells in plates, and mumps-specific igm was detected by adding mumps virus hemagglutinin and guinea pig erythrocytes consecutively. specific igm-positive sera showed hemadsorption, whereas negative sera showed hemagglutination. all 81 patients with current mumps infections tested showed mumps-specific igm antibody, with titers ranging fr ... | 1982 | 6313743 |
| myxoviruses do not induce non-specific alterations in membrane permeability early on in infection. | the permeability characteristics of cells infected with myxoviruses have been studied by measuring the concentrative uptake of nutrients, the concentration of intracellular k+, and the maintenance of the na+ gradient across the plasma membrane. cells either show no change at all (sendai virus-infected bhk cells and measles virus-infected vero cells) or they show a decreased ability to concentrate nutrients, while intracellular k+ and the na+ gradient remain unchanged (sendai and influenza virus- ... | 1983 | 6314933 |
| multiple viruses in diabetes mellitus. | | 1984 | 6322233 |
| depression of the lymphocyte transformation response to microbial antigens and to phytohemagglutinin during pregnancy. | lymphocyte transformation (lt) responses to chlamydia trachomatis, to four other microbial antigens, and to phytohemagglutinin (pha) were studied in 201 women during pregnancy and/or 3-18 wk postpartum. the lt responses to all stimulants tested were significantly depressed during pregnancy when compared with postpartum lt responses. this difference occurred whether lt assays were performed in autologous or pooled heterologous plasma collected from nonpregnant donors. among women studied in the t ... | 1983 | 6355182 |
| detection and identification of virus-specific, oligoclonal igg in unconcentrated cerebrospinal fluid by immunoblot technique. | a technique is described which allows the detection of virus-specific oligoclonal igg in unconcentrated cerebrospinal fluid (csf) from patients with virus infections of the central nervous system. csf is isoelectrically focused in agarose gels and immunoglobulins are blotted to nitrocellulose filters, passively loaded with either anti-human igg or viral antigen. transferred total igg, as well as virus-specific igg, is identified by the use of peroxidase-labelled anti-human igg and 4-chloro-1-nap ... | 1984 | 6511890 |
| sensitive hemagglutination inhibition test for mumps antibody. | incorporation of heterologous immunoglobulin against human immunoglobulin g into the mumps hi tests provided a highly sensitive and specific way of measuring mumps antibody in human sera. the sensitivity of this enhanced mumps hi test compared favorably with that of an anti-immunoglobulin-enhanced virus plaque neutralization test, but the hi test required considerably less time and effort to perform. | 1981 | 6972385 |
| [growth of mumps virus in human stomach cancer cell lines. i. establishment of persistent infections (author's transl)]. | human stomach cancer cells (mkn, kato cells) were infected with urabe strain and wild type of mumps virus at an input multiplicity of 0.01. both urabe strains and wild type of mumps virus propagated rapidly in both cells and more than 10(7) p.f.u./ml of infectious virus was released. moreover, all cultures were led to the establishment of persistent infection. these persistently infected cells were maintained under the absence of anti-serum. in all of the cultures, mumps viral antigens were dete ... | 1981 | 7327506 |
| the role of viral glycoproteins in mumps-virus-dependent lymphocyte-mediated cytotoxicity in vitro. | human peripheral blood lymphocytes (pbl) from healthy donors express enhanced natural cytotoxicity to target cells after a brief exposure to mumps virus in vitro. we describe here experiments aiming at elucidating the mechanism of this virus-dependent cytotoxicity. treatment with proteolytic enzymes resulted in virus particles depleted of one or both kinds of their glycoproteins spikes. removal of both of these components frrom the virion abrogated their ability to enhance cytotoxicity. this vir ... | 1980 | 7384752 |
| common peptide epitope in mumps virus nucleocapsid protein and mhc class ii-associated invariant chain. | the present study describes a 7 amino acid-long sequence (yqqqgrl) which is identical in hla-associated invariant chain and mumps virus nucleocapsid protein and is additionally followed by one conservative amino acid pair. as such a long amino acid homology is extremely rare in two evolutionarily unrelated proteins the possibility that it could induce immunological cross-reactivity was evaluated. several antigenicity indices suggested high antigen potential within this region. synthetic peptides ... | 1993 | 7683440 |
| ubiquinone-10 protects neurons from virus-induced degeneration. | cultured neurons from rat dorsal root ganglia and cerebral cortex were infected with sendai virus, which gives a productive replication with lysis of most neurons, and with the rw strain of mumps virus, which undergoes defective replication causing degeneration of only 30-40% of the neurons within 5 days after initial infection. in sendai virus-infected cells the amount of polyisoprenoid lipids was enhanced. in mumps virus-infected cultures there were transient reductions in the contents of chol ... | 1994 | 8035187 |
| [pathogenesis of congenital hydrocephalus: role of transplacental myxovirus infection]. | several pediatric cases of hydrocephalus after mumps virus infection, have been reported. these cases are thought to be caused by ependymitis due to mumps virus infection. clinical cases of congenital hydrocephalus possibly caused by intrauterine mumps virus infection are also accumulating. however, it has not yet been made clear whether mumps virus can pass through the human placenta and cause hydrocephalus. our experimental studies demonstrated that mumps and parainfluenza virus type 3 could i ... | 1994 | 8185971 |
| measles, mumps and rubella immunisation in children at risk of infection with human immunodeficiency virus. | a retrospective serological study of 81 children at risk of hiv (ii hiv-infected, 70 hiv-negative) was performed to assess susceptibility to measles, mumps and rubella and response to measles, mumps and rubella (mmr) immunisation. there was no difference in the initial serological response between the hiv-infected and hiv-negative children. repeat serology should be performed on hiv-infected children as during follow-up antibodies may be lost. reimmunisation should be considered for the seronega ... | 1993 | 8308316 |
| isolation of parainfluenza virus type 3 from cerebrospinal fluid associated with aseptic meningitis. | parainfluenza virus type 3 has been isolated from the cerebral spinal fluid (csf) from six individuals--four children and two adults--over a 10-year period. all had fever, and four had signs of meningitis. all recovered uneventfully, including one child undergoing chemotherapy for medulloblastoma. the clinical presentation of this child who developed parainfluenza virus type 3 meningitis is described, and the cases of five other individuals with parainfluenza virus type 3 isolated from the csf a ... | 1993 | 8391749 |
| laboratory surveillance of viral meningitis by examination of cerebrospinal fluid in cape town, 1981-9. | nine years accumulated laboratory data derived from the culture of the cerebrospinal fluid of 11,360 aseptic meningitis cases were retrospectively reviewed to establish the epidemiology of viral meningitis in cape town. virus was isolated from 3406 of the cases (91% enteroviruses and 9% mumps). five major summer viral meningitis episodes were documented: two of echovirus 4 (706 and 445 cases), echovirus 9 (223), coxsackie a9 (104) and one of unidentified enterovirus (324 cases--probably echo 9). ... | 1993 | 8405162 |
| [gianotti-crosti syndrome due to a mixed infection produced by the mumps virus and the parainfluenza virus type 2]. | | 1996 | 8849065 |
| antigenic and genetic variation of the hn protein of mumps virus strains. | the relationships between different strains of mumps virus were established by determination of the sequence of the hn gene. they closely resemble those established from other portions of the genome, suggesting that the viruses are not recombinants over the areas examined. the relationships were consistent with those established by reaction with monoclonal antibodies raised against the urabe strain, which has a similar antigenic structure to previously studied laboratory strains, and largely con ... | 1996 | 8887482 |
| cerebrospinal fluid viral antibodies in obsessive-compulsive disorder in an indian population. | immunoglobulin g viral antibodies for herpes simplex virus type 1, varicella zoster virus, cytomegalovirus, measles, and mumps were studied in 76 subjects with obsessive-compulsive disorder and compared with a control population. there was a significantly higher titer for herpes simplex virus type 1 antibodies. sera: cerebrospinal fluid ratios were suggestive of intrathecal synthesis. | 1997 | 9099415 |
| igm antibodies in hospitalized children with febrile illness during an inter-epidemic period of measles, in somalia. | in the context of the measles elimination programme, endorsed by the expanded programme on immunization of the world health organization, the identification of infections which may be confused with measles is a major challenge. | 1999 | 10073410 |
| [immunodepressive effect of measles and parotitis virus in vitro]. | mechanisms of suppressive effect of measles and parotitis virus on lymphocyte proliferation and cytolytic activity of natural killers are studied in vitro. both viruses exert a weak mitogenic effect on human blood lymphocytes and at the same time suppress their response to phytohemagglutinin stimulation in vitro. suppressive effect of measles virus is more expressed than of parotitis virus. moreover, measles virus suppresses lysis of human blood killer target cells. the suppressive effect manife ... | 1999 | 10190242 |
| augmentation of verotoxin-induced cytotoxicity/apoptosis by interferon is repressed in cells persistently infected with mumps virus. | verotoxin type 2 (vt2) produced by enterohemorrhagic escherichia coli (ehec) has been shown to have high cytotoxic potency toward several human b lymphoid cell lines with and without epstein-barr virus (ebv). cell death, apoptosis induced by vt2, is closely correlated with the expression of receptor molecule gb3/cd77, recognized by the toxin, but not with the infection or presence of ebv. pretreatment of cells with interferon-alpha (ifn-alpha) for 24 h resulted in augmentation of apoptosis by vt ... | 1999 | 10386860 |
| no changes in paired viral antibody titers during the course of acute schizophrenia. | although there have been many studies surveying the prevalence of specific viral antibodies in a large cohort of patients with schizophrenia, changes in antibody levels during the course of acute illness have not been fully investigated. we conducted a preliminary study investigating levels of antibodies to 5 herpesviruses (herpes simplex virus type 1, cytomegalovirus, epstein-barr virus, varicella-zoster virus and human herpesvirus type 6) and 6 other viruses (measles, rubella, mumps, influenza ... | 1999 | 10474057 |
| synthesis and antiviral activity of some n-benzenesulphonylbenzimidazoles. | some n-sulphonylated benzimidazoles were synthesized as potential antiviral agents. compound 16b and, to a lesser extent, 19b showed activity against two rna viruses at micromolar concentrations. | 1999 | 10498201 |