| comparison of montana and alaska isolates of echinococcus multilocularis in gerbils with observations on the cyst growth, hook characteristics, and host response. | to assess its biological distinctness, an isolate of echinococcus multilocularis from montana was compared with an isolate from alaska in gerbils (meriones unguiculatus) by means of intraperitoneal inoculations with protoscoleces. the cysts formed by the montana isolate were entire, hyaline, and translucent, whereas those produced by the alaska isolate were granular, yellowish, and opaque. vesicles of the montana isolate were larger, produced protoscoleces more slowly but in greater numbers, and ... | 1992 | 1597801 |
| echinococcus multilocularis--a model for imaging research. | magnetic resonance images of transverse, sagittal, and coronal sections of meriones unguiculatus abdomens experimentally infected with echinococcus multilocularis revealed alveolar cyst masses that were especially prominent in the late phase of infection. | 1991 | 1919937 |
| uniform strobilar development of echinococcus multilocularis in vitro from protoscolex to immature stages. | the aim of this study was to obtain uniformity in strobilar development of echinococcus multilocularis from protoscoleces in vitro. the isolate of e. multilocularis used was derived initially from a human case in france and subsequently maintained in the laboratory by intraperitoneal passage in meriones unguiculatus. protoscoleces used for culture were obtained using preparative procedures in which parasite tissue was disrupted gently with minimal exposure to pepsin and acidic conditions followe ... | 1990 | 2319425 |
| proliferation and metastases formation of larval echinococcus multilocularis. i. animal model, macroscopical and histological findings. | using surgical techniques, 70 meriones unguiculatus were infected by implantation of 0.15-0.20 g of larval echinococcus multilocularis tissue into the subcutis of the neck region. in 64 of 65 animals necropsied, the transplants had increased in size and weight and reached an average weight of 4.1 g at the end of the experiment 12 weeks post infection (p.i.). metastatic echinococcus lesions developed in the regional lymph nodes and in the lungs; in 18 animals the parasite proliferated into the th ... | 1983 | 6659651 |
| [generalized amyloidosis in the mongolian gerbil (meriones unguiculatus) following infection with echinococcus multilocularis larvae]. | | 1982 | 6817554 |
| synthesis of epoxypropyloxy chromones and allyl hydroxy chromones and in vitro-in vivo activity on protoscoleces of echinococcus multilocularis (cestoda). | derivatives with epoxypropyloxy, allyloxy and allyl hydroxychromone structures were synthesized and tested against protoscoleces of echinococcus multilocularis. compounds iv-ix were tested in vitro and compound vii proved the most active of them with, at 0.1 mmol.l-1, 98% dead protoscoleces in open vesicles after 24 hours and 80% in closed vesicles within 96 hours. compound vii was tested in vivo on meriones unguiculatus infested by echinococcus multilocularis, but was found to be rather inactiv ... | 1993 | 8373504 |
| infectivity of hymenolepis diminuta for the jird, meriones unguiculatus, and utility of this model for anthelmintic studies. | the jird (meriones unguiculatus) has been shown to be a useful model host for the cestodes taenia crassiceps and echinococcus multilocularis. this report outlines a novel model in which hydrocortisone-treated jirds (0.02% in the feed) are infected with another cestode, hymenolepis diminuta. jirds were inoculated with 5 freshly harvested cysticercoids of h. diminuta prior to (day 0, -1, or -5) or after (day 1 or 5) switching to medicated feed; in some cases, jirds were never medicated. on days 7, ... | 1996 | 8636859 |
| alteration in brain metabolites of jirds infected with alveolar echinococcus. | the meriones unguiculatus-echinococcus multilocularis host-parasite system was used to evaluate changes in metabolite levels in the brain of the infected host. nuclear magnetic resonance analysis of perchloric acid extracts of the entire brain of jirds revealed that infection caused a change in the concentration of several metabolites, especially those that serve as amino acidergic neurotransmitters in this organ. the concentrations of the neuroexcitatory compounds glutamate and aspartate and th ... | 1997 | 9267426 |
| metabolic alterations in organs of meriones unguiculatus infected with echinococcus multilocularis. | 1. 1h-nmr spectra of liver, spleen and kidney extracts from meriones unguiculatus infected with echinococcus multilocularis showed decreased levels of glucose. in addition, the liver extracts were severely glycogen-depleted. 2. both livers and spleens contained less glycine, taurine and acetate. spleens also had fewer cholines and less lactate but more betaine and alanine. 3. in the kidneys, elevated concentrations of succinate, acetate and lactate were found. | 1993 | 8365107 |
| synthesis of propargylic alcohols and biological effects on echinococcus multilocularis metacestodes. | this study describes the synthesis of propargylic alcohols derived from isatin and their biochemical and morphological effects on echinococcus multilocularis metacestodes in meriones unguiculatus. propargylic alcohols decreased the alkaline phosphatase and the lactate dehydrogenase activities of the metacestode selectively. the most effective compound, 1b, decreased the lactate dehydrogenase enzymatic activity, and the glucose concentration in the parasite increased, whereas the glycogen content ... | 1993 | 8331534 |
| deficiency of antibody responses to t-independent antigens in gerbils---meriones unguiculatus. | meriones unguiculatus commonly known as gerbils are widely used as animal models for a variety of parasitic infections such as brugia malayi, entamoeba histolytica, giardia duodenalis, toxoplasma gondi, helicobacter pylori, strongyloides stercoralis and echinococcus multilocularis. groups of balb/c mice, gerbils and xid mice were studied for antibody responses to t-independent antigens. gerbils were found to be significantly deficient in eliciting antibodies to both dextran and phosphorylcholine ... | 2002 | 11888652 |
| the susceptibility of the mongolian gerbil (meriones unguiculatus) and the of mouse strain to echinococcus multilocularis--ultrastructural aspects of the cysts. | | 1987 | 3570643 |
| proliferation and metastases formation of larval echinococcus multilocularis. ii. ultrastructural investigations. | the larval stage (metacestode) of echinococcus multilocularis was studied by means of electron microscopy (sem, tem) before and after subcutaneous transplantation to jirds (meriones unguiculatus) and in their lymph nodes and lungs with parasite metastases. it was found that the metacestode consists of a network of solid, cellular protrusions (buds) of the germinal layer which transform to tube-like and cystic structures devoid or with a laminated layer. proliferation of the metacestode apparentl ... | 1983 | 6659652 |
| the maintenance of echinococcus multilocularis in gerbils (meriones unguiculatus) by intraperitoneal inoculation. | | 1961 | 14480353 |
| echinococcus multilocularis: responses to infection in mongolian gerbils. | mongolian gerbils (meriones unguiculatus) inoculated intraperitoneally with three acephalic cysts of echinococcus multilocularis were very susceptible to infection. aspects of the responses of gerbils to this infection were examined to determine if they could be related to the progress of the infection. hematologic changes observed during the infection included anemia, reticulocytosis, lymphocytopenia, neutrophilia, monocytosis, and eosinopenia; these changes were related to the size of the infe ... | 1986 | 3943584 |
| demonstration of a specific echinococcus multilocularis antigen in the supernatant of in vitro maintained protoscolices. | serodiagnosis of echinococcosis is still often met with difficulties resulting from unspecific reactions due to crude antigens which contain numerous host-derived proteins. in order to eliminate host protein contamination we produced echinococcus multilocularis antigen by methods of in vitro technique: evaginated protoscolices of echinococcus multilocularis isolated from experimentally infected mongolian gerbils (meriones unguiculatus) were maintained in rpmi 1640 medium. although no serum prote ... | 1988 | 3407363 |
| in vitro activities of itraconazole, methiazole, and nitazoxanide versus echinococcus multilocularis larvae. | albendazole (abz) and mebendazole are the only drugs licensed for treatment of human alveolar echinococcosis. in order to augment the armamentarium against this deadly disease, we tested a series of drugs for their efficacy against echinococcus multilocularis larvae. e. multilocularis larvae grown intraperitoneally in mongolian gerbils were transferred into tissue culture. vesicles budded from the tissue blocks and after 6 weeks, drugs were added, and the effect on the vesicles was observed. we ... | 2006 | 16940089 |
| echinococcus multilocularis: comparative study of glycogen synthase in metacestodes and in the livers of infected and control meriones unguiculatus. | a comparative study of the kinetic parameters of glycogen synthase was performed on echinococcus multilocularis metacestodes and on the livers of infected and control host (meriones unguiculatus). the enzyme of the parasite was found to be different from the enzyme of infected host liver. the apparent km for udp-glucose is 100 microm for the parasite and 400 microm for the host liver. the apparent km for glucose 6-phosphate is 4 mm for the parasite and 2 mm for the host liver. the apparent km fo ... | 1988 | 3130271 |
| the first report in turkey of in vivo cultivation in rattus norvegicus of echinococcus multilocularis human strain. | the adult form of the small cestode echinococcus multilocularis is found in carnivorous animals, especially in the fox. this cestode, which is found in the northern hemisphere in the world, is the cause of a generally fatal disease in humans, known as alveolar echinococcosis (ae). the metacestodes of echinococcus multilocularis can be experimentally developed in the meriones unguiculatus species of rodents, and it is possible to use these metacestodes for numerous purposes, primarily for supplyi ... | 2007 | 17918057 |
| streptococcus merionis sp. nov., isolated from mongolian jirds (meriones unguiculatus). | gram-positive, catalase-negative, chain-forming, coccus-shaped organisms were isolated both from intraperitoneally grown vesicles of the fox tapeworm echinococcus multilocularis and the oropharynges of laboratory-kept mongolian jirds (meriones unguiculatus). the strains displayed no haemolytic activity on columbia sheep blood agar, pyrrolidonyl arylamidase activity was negative and the organisms reacted weakly with lancefield group d antiserum. on the basis of phenotypic characteristics, the str ... | 2009 | 19329603 |
| a comparative study of utp-d-glucose-1-phosphate uridylyl transferase in the cysts of echinococcus multilocularis and the livers of infected and control meriones unguiculatus. | kinetic and physical parameters of udp-glucose pyrophosphorylase were determined in meriones unguiculatus infected with echinococcus multilocularis metacestodes (cestoda). studies were carried out on parasite cysts, and on livers from control and infected animals after purification of the enzyme by affinity chromatography on utp-agarose. the enzyme from infected and control livers had km values for utp of 0.01 mm and 0.5 mm, respectively; for glucose-1-phosphate values were 0.46 mm and 0.07 mm, ... | 1987 | 3033498 |
| echinococcus multilocularis metacestodes: biochemical and ultrastructural investigations on the effect of isatin (2-3 indoline dione) in vivo. | a study on the biochemical and ultrastructural effects of isatin on echinococcus multilocularis metacestodes in meriones unguiculatus is reported. in the metacestode, after an 18 day treatment period, the alkaline phosphatase and lactate dehydrogenase activities decreased and the acid phosphatase activity increased. glucose and glycogen stores declined significantly. examinations of the ultrastructure revealed an increase of lysosomes and phagocytic reactions. the protoscolesces were damaged and ... | 1989 | 2708182 |
| effects of ethyl n-n-benzyl-methyl-oxamate in meriones unguiculatus infected with echinococcus multilocularis metacestodes. biochemical and ultrastructural observations. | ethyl n-n-benzyl-methyl-oxamate is a n-substituted derivative of the oxamic acid, well-known as inhibitor of the lactate dehydrogenase activity. the biochemical and ultrastructural effects of this drug in echinococcus multilocularis metacestodes and the repercussions in the livers of receptive host, meriones unguiculatus, were investigated. this compound decreased the specific activity of the lactate dehydrogenase in the metacestodes and the host liver by 81 and 86.8%, respectively. this n-methy ... | 1990 | 2383305 |
| laboratory rodent models for the tapeworm-stage of taenia saginata and other related taeniid species. | attempts were carried out to establish alternative definitive host systems for taenia saginata, t. crassiceps, t. hydatigena, echinococcus multilocularis and e. granulosus, in rodents. following oral inoculation of cysticerci or protoscoleces, the tapeworm-stage development and sexual maturation of the 5 taeniid species in the intestine of a golden hamster (gh) and mongolian gerbil (mg), both treated with prednisolone, were examined. sexually mature t. saginata tapeworms were recovered from mg p ... | 1991 | 1822901 |
| enhanced larval cyst growth of echinococcus multilocularis in praziquantel-treated jirds (meriones unguiculatus). | jirds (meriones unguiculatus) inoculated intraperitoneally with cystic material of echinococcus multilocularis were given daily oral treatments of praziquantel at 300 mg/kg of body weight (bw) or dimethyl sulfoxide vehicle for five-day treatment regimens starting at 29 days postinoculation (pi) up to 69 days pi. at 39 or 49 days pi, the growth of the larval cystic mass (lcm) in jirds following a single or two five-day treatment regimens was significantly enhanced (p < 0.05) by 129.0% (2.3-fold) ... | 1994 | 8304567 |
| comparison of the viability and developmental characteristics of echinococcus multilocularis isolates from human patients in france. | alveolar echinococcosis, due to e. multilocularis, is usually a fatal disease in patients whether treated by benzimidazolecarbamates or not. however, aborted infections have been described, suggesting the existence of strains of parasites of varying pathogenicity. these observations led us to analyse the viability of larvae in 20 patients. after observation of human lesions, the viability of metacestodes was tested by intraperitoneal infection in two intermediate host species, meriones unguicula ... | 1990 | 2312231 |
| the first report of echinococcus multilocularis strain isolation from human in turkey. | the adult form of the small cestode echinococcus multilocularis (e. multilocularis) is found in carnivorous animals, especially in the fox. this cestode, which is observed in the northern hemisphere of the world, is the cause of a generally fatal, progressing disease in humans, known as "alveolar echinococcosis" (ae). the metacestodes of e. multilocularis can be experimentally developed in the meriones unguiculatus species of rodents, and it is possible to use these metacestodes for numerous pur ... | 2005 | 17167740 |
| effects of albendazole on echinococcus multilocularis infection in the mongolian jird. | albendazole chemotherapy of larval echinococcus multilocularis was studied in the mongolian jird by administration of medicated feed at various concentrations and durations. the effects were evaluated by comparison of treated and control groups in terms of host mortality, larval metastases to the lungs, and final weight and histologic appearance of larval tissue. viability of larval tissue at necropsy of each animal was tested by inoculation into two noninfected jirds. albendazole-medicated feed ... | 1990 | 2230275 |
| in vivo morphological damage induced by a new benzimidazole prodrug in echinococcus multilocularis metacestodes. | benzimidazoles are the most widely used compounds in chemotherapy of alveolar hydatid disease (ahd), but long-term administration is required to reach detectable plasma levels. n-methoxycarbonyl n'-2-nitro 4-trifluoromethyl phenyl thiourea (2) was prepared as a potential prodrug of (5-trifluoromethyl-1h-benzimidazole-2-yl)-carbamic acid methyl ester. biological effects of 2 were evaluated in gerbils (meriones unguiculatus) against the causative agent of ahd, echinococcus multilocularis metacesto ... | 1990 | 2118956 |
| detection of antibodies against glycolipids of echinococcus multilocularis metacestodes in sera of patients with alveolar hydatid disease. | glycosphingolipids extracted from echinococcus multilocularis metacestodes were tested against sera from patients with alveolar hydatid disease (ahd). the tests were performed first by elisa then by immunostaining on thin layer chromatography. a binding between the parasite glycolipids and antibodies of ahd human sera was observed by elisa. the glycolipids related to this binding were specifically parasite neutral glycosphingolipids. no reaction was observed with parasite acid glycolipids nor wi ... | 1991 | 1923563 |
| complete life cycle of the canid tapeworm, echinococcus multilocularis, in laboratory rodents. | mongolian gerbils, meriones unguiculatus, when treated at intervals of 2-6 days with prednisolone tertiary butyl acetate, sustained infection with adult echinococcus multilocularis in the small intestine, with the tapeworm exhibiting normal strobilation and egg production as in the natural canid host. host age is critical for the survival of the tapeworm in normal gerbils; parasites survive for only 2 days in 20-wk-old animals, 4 days in 4-wk-old animals, but at least 7 days in 3-wk-old animals. ... | 1990 | 2253847 |
| metabolites of alveolar echinococcus as determined by [31p]- and [1h]-nuclear magnetic resonance spectroscopy. | [31p]-nuclear magnetic resonance (nmr) in vivo spectra of echinococcus multilocularis cysts growing subcutaneously in meriones unguiculatus showed prominent signals due to phosphomonoesters (pme), phosphodiesters (pde), inorganic phosphate (pi) and the alpha, beta and gamma phosphate groups of adenosine triphosphate (atp). the internal ph of the parasite cysts was 6.7-6.8. the 31p spectra of extracts of these subcutaneous cysts showed peaks identified as glucose-6-phosphate (glu-6-p), glycerol-3 ... | 1992 | 1480603 |
| glycosphingolipids of echinococcus multilocularis metacestodes. | neutral and acid glycosphingolipids of echinococcus multilocularis metacestodes that were obtained after intraperitoneal infection of meriones unguiculatus have been analyzed by thin layer chromatography. neutral and acid glycosphingolipids accounted for 95% and 5% of total glycosphingolipids, respectively. 12 different fractions were observed in the neutral glycosphingolipids extracts of the parasite. the most important was a monohexosylceramide fraction accounting for 56.4% of neutral glycosph ... | 1990 | 2320054 |
| chemotherapy of experimental echinococcus multilocularis in jirds. | a total of 50 jirds (meriones unguiculatus) were infected by intraperitoneal implantation of 0.20 g metacestode tissue of echinococcus multilocularis. the feed of 4 groups of 10 animals each was treated for 35 days with 500 ppm albendazole, flubendazole, mebendazole or praziquantel; 1 group of 10 jirds served as unmedicated controls. at autopsy 2 weeks after the end of medication, the transplants had increased in size and weight, reaching a total weight of 78.11 g in the control group, 21.60 g i ... | 1990 | 2315283 |
| [effect of thermochemotherapy on alveolar hydatid disease of the liver]. | the specific aim of this study is to predict the possibility of local hyperthermia as a therapeutic modality for echinococcus multilocularis of the liver. we used meriones unguiculatus, into which larval e. multiocularis, alaska strain, was innoculated. in the experiments of hyperthermia by low output laser for either innoculated liver lesions or the normal liver tissues, the protoscoleces and the germinal layers are destroyed at 41 degrees c for 15 minutes, and normal hepatocytes irreversibly d ... | 1992 | 1552887 |
| purine metabolism in echinococcus multilocularis. | the activities of the enzymes in echinococcus multilocularis metacestodes involved in purine salvage were studied by hplc. as in most parasites, this cestode relies entirely on salvage of preformed bases and nucleosides for its purine requirement. therefore, these enzymes may be targets for drugs in the chemotherapeutic treatment of diseases caused by this parasite. the animals used in this study were gerbils (meriones unguiculatus). enzyme activities from sera and hepatic tissue in control and ... | 1998 | 9854810 |
| in vitro activities of benzimidazoles against echinococcus multilocularis metacestodes. | alveolar echinococcosis, caused by the larval (metacestode) stage of the tapeworm echinococcus multilocularis, is a lethal parasitosis of the liver prevalent in the northern hemisphere. for chemotherapy the benzimidazole derivatives mebendazole and albendazole were introduced, and their use has resulted in a significant improvement in the survival rates. however, data from experiments with animals and clinical observations indicate that these drugs elicit only parasitostatic activity and in most ... | 1998 | 9593125 |
| establishment of a primary culture of echinococcus multilocularis germinal cells. | this study was designed to establish an in vitro primary culture of germinal cells of echinococcus multilocularis, a parasite that causes alveolar echinococcosis of the liver (ael). we also investigated the temperature-dependency of the cultured cells. the germinal cells, which originated from a human lesion, were cultured by an original fluid-suspension method at 25 degrees c or 37 degrees c for 4 weeks. anchorage-dependent and -independent cells were observed by light microscopy, transmission ... | 1997 | 9213248 |
| natural echinococcus multilocularis infection in a norway rat, rattus norvegicus, in southern hokkaido, japan. | forty-two rats, rattus norvegicus, captured at a garbage dump in southern hokkaido, japan, were examined, and one was found to be infected with echinococcus multilocularis. the lesions were found in the liver, lung, mesenteric lymph nodes, greater omentum and also free in the abdominal cavity. no necrosis was observed in any of the lesions, and inflammatory reactions were mild. protoscoleces were observed in the large liver cysts. a homogenate of these cysts, when transplanted into the abdominal ... | 1992 | 1399254 |
| dose dependency of prednisolone tertiary-butylacetate (ptba) treatment on the establishment and site predilection of echinococcus multilocularis in an alternative definitive host model using mongolian gerbil (meriones unguiculatus). | mongolian gerbils (meriones unguiculatus) were orally inoculated with 10,000 protoscoleces of echinococcus multilocularis per head after being divided into five groups (a e). each group was dosed with prednisolone tertiary-butylacetate (ptba) as follows: a, 0 mg; b, 0.5 mg; c, 2 mg; d, 5 mg; and e, 10 mg/head. all animals were injected subcutaneously with control solvent or ptba every other day from 6 days pre- to 6 days post-infection. autopsy was performed at 7 days post-infection. doses of pt ... | 2000 | 10894482 |
| effect of echinococcus multilocularis on the origin of acetyl-coa entering the tricarboxylic acid cycle in host liver. | carbon-13 nuclear magnetic resonance (nmr) spectroscopy was employed to investigate alterations in hepatic carbohydrate metabolism in meriones unguiculatus infected with echinococcus multilocularis. following portal vein injections of an equimolar mixture of [1,2-13c2]acetate and [3-13c]lactate, perchloric acid extracts of the livers were prepared and nmr spectra obtained. isotopomer analysis using glutamate resonances in these spectra showed that the relative contributions of endogenous and exo ... | 2002 | 12018193 |
| effect of amphotericin b on larval growth of echinococcus multilocularis. | alveolar echinococcosis is caused by the parasitic cestode echinococcus multilocularis. benzimidazoles, namely, mebendazole and albendazole, are the only drugs available for the treatment of inoperable alveolar echinococcosis. at present, no therapeutic alternative is available for patients with progressive disease under treatment or for patients who are unable to tolerate the side effects of the benzimidazoles. in addition, benzimidazoles are only parasitostatic for e. multilocularis. thus, new ... | 2003 | 12543669 |
| immune responses to oral infection with echinococcus multilocularis protoscoleces in gerbils: modified lymphocyte responses due to the parasite antigen. | immune responses to oral infection with echinococcus multilocularis protoscoleces in mongolian gerbils were investigated. gerbils not treated with prednisolone expelled most of the parasites within 3 days post-infection and induced parasite-specific intestinal iga secretion after the oral inoculation with protoscoleces. in contrast, prednisolone-treated gerbils harbored notable numbers of parasites, and the parasite-specific intestinal iga secretion was lower. in gerbils not treated and orally i ... | 2005 | 15765248 |
| [scanning electron microscope studies of echinococcus multilocularis cysts (author's transl)]. | in the present paper, morphologic studies on experimental echinococcosis are reported. following i.p. infection of gerbils (meriones unguiculatus) with echinococcus multilocularis scolices, the development of cysts within the internal organs of the experimental animals could be readily visualized by scanning electron microscopy. | 1976 | 1016476 |
| mesenteric doppler ultrasonography findings of echinococcus multilocularis infection: an experimental study. | the diagnosis of alveolar echinococcosis, caused by the larval stage of echinococcus multilocularis, is often difficult and almost always possible only in the later stages of the disease. in this study, we aimed to evaluate the findings of doppler ultrasonography images for the diagnosis of intraabdominal non-visceral alveolar echinococcosis in meriones unguiculatus. six infected animals were studied. abdominal doppler ultrasonography examinations were performed 20-25 days after the implantation ... | 2009 | 19598092 |
| dose dependency of prednisolone on the establishment of echinococcus multilocularis infection in an alternative definitive host, mongolian gerbil. | this study revealed the dose dependency of prednisolone tertiary-butylacetate (ptba) treatment on the establishment of echinococcus multilocularis in the small intestine of mongolian gerbil (meriones unguiculatus) and that some of the physiological parameters of host were correlated with the doses of ptba and establishment of the worm. twenty mongolian gerbils were divided into 5 groups, according to the doses of ptba; 0 mg, 0.5 mg, 2 mg, 5 mg and 10 mg per head. all animals were injected intrap ... | 2000 | 10842921 |
| increases in the effects of albendazole on echinococcus multilocularis metacestodes by the dipeptide methyl ester (phe-phe-ome). | three months after infection with echinococcus multilocularis, mongolian gerbils were given either the dipeptide methyl ester (phe-phe-ome) or a combination of phe-phe-ome plus albendazole to treat alveolar echinococcosis. each drug was given orally at the daily dose of 50 mg/kg of body weight following various administration regimens. histologic and ultrastructural studies of parasites recovered from infected gerbil tissues showed that the dipeptide methyl ester increases the effect of albendaz ... | 1997 | 9080884 |
| development of a movement-based in vitro screening assay for the identification of new anti-cestodal compounds. | intestinal cestodes are infecting millions of people and livestock worldwide, but treatment is mainly based on one drug: praziquantel. the identification of new anti-cestodal compounds is hampered by the lack of suitable screening assays. it is difficult, or even impossible, to evaluate drugs against adult cestodes in vitro due to the fact that these parasites cannot be cultured in microwell plates, and adult and larval stages in most cases represent different organisms in terms of size, morphol ... | 2017 | 28520724 |
| a new technique to avoid losing the strains of echinococcus multilocularis during passaging. | to examine the survival of echinococcus multilocularis (e. multilocularis) and the formation of protoscoleces, under refrigerated conditions over a 43-day observation period. | 2005 | 16380771 |
| effects of in vitro exposure of echinococcus multilocularis metacestodes to cytostatic drugs on in vivo growth and proliferation of the parasite. | the cytostatic drugs vincristine (vcr), navelbine (nav), and methotrexate (mtx) were evaluated for their growth inhibitory potential against metacestodes of echinococcus multilocularis (em) by in vitro and in vivo assays. in vitro cultures of e. multilocularis were exposed to ic 90, ic 80, and ic 5 concentrations of vcr, nav, or mtx for 1 week, then parasite tissue cultures were kept for 1 week without drug exposure in vitro, and thereafter, metacestode tissues were injected intra-peritoneally i ... | 2010 | 20461408 |
| subcutaneous infection model facilitates treatment assessment of secondary alveolar echinococcosis in mice. | alveolar echinococcosis (ae) in humans is a parasitic disease characterized by severe damage to the liver and occasionally other organs. ae is caused by infection with the metacestode (larval) stage of the fox tapeworm echinococcus multilocularis, usually infecting small rodents as natural intermediate hosts. conventionally, human ae is chemotherapeutically treated with mebendazole or albendazole. there is, however still the need for improved chemotherapeutical options. primary in vivo studies o ... | 2013 | 23717701 |