| cell differentiation-related gene expression of human papillomavirus 33. | the gene expression of human papillomavirus (hpv) 33, which can be detected both in benign and malignant genital tumors, was analyzed in a cervical condyloma acuminatum by in situ hybridization using open reading frame-specific rna probes. viral mrna concentrations increased with the degree of differentiation of the keratinocytes. the probes for reading frames e4 and e5 generated the most intense signals. the patterns of the specific viral mrnas were very similar to those in condylomas induced b ... | 1990 | 2175837 |
| binding and internalization of human papillomavirus type 33 virus-like particles by eukaryotic cells. | infection of cells by human papillomaviruses (hpvs) associated with malignant genital lesions has not been studied because of the lack of an in vitro system and the unavailability of virions. we have now used virus-like particles (vlps) of hpv type 33 to analyze the initial events in the interaction of the hpv capsid with cell lines. binding of vlps to hela cells was observed in biochemical assays and by immunofluorescence. vlp binding was inhibited by antisera raised against vlps but not by mon ... | 1995 | 7745672 |
| benign leydig cell tumor of the testis associated with human papillomavirus type 33 presenting with the sign of leser-trélat. | we present a case of a benign testicular leydig cell tumor and eruptive seborrheic keratosis associated with human papillomavirus type 33 infection. to our knowledge this is the first occurrence of a leydig cell tumor in a patient with tuberous sclerosis and the second documented case of eruptive seborrheic keratoses (sign of leser-trélat) associated with a benign neoplasm. | 1993 | 8396689 |
| conjunctival papillomas caused by human papillomavirus type 33. | | 2002 | 11831924 |
| dissection of human papillomavirus type 33 l2 domains involved in nuclear domains (nd) 10 homing and reorganization. | we have recently shown that the minor capsid protein l2 of human papillomavirus type 33 (hpv33) recruits the transcriptional repressor daxx into nuclear domains (nd) 10 and causes the loss of the transcriptional activator sp100 from these subnuclear structures. in order to dissect l2 domains involved in nuclear translocation, nd10 homing, loss of sp100, and recruitment of daxx, a detailed deletion mutagenesis of l2 was performed. using immunofluorescence and green fluorescent protein fusions, we ... | 2003 | 14517069 |
| pseudovirions as specific tools for investigation of virus interactions with cells. | this chapter outlines the generation and application of human papillomavirus type 33 (hpv33) pseudovirions. the method describes (1) the construction of vaccinia viruses recombinant for the major and minor hpv capsid proteins, l1 and l2, respectively; (2) the transfection of cos7 cells with a marker plasmid replicating to high copy numbers; (3) the expression of l1 and l2 using the vaccinia virus expression system; (4) the extraction, purification, and analysis of hpv33 pseudovirions; and (5) th ... | 2005 | 15507710 |
| generation and applications of hpv pseudovirions using vaccinia virus. | this chapter outlines the generation and application of human papillomavirus type 33 (hpv 33) pseudovirions. these pseudovirions are structurally indistinguishable from native virions and are therefore valuable tools for the study of papillomavirus/cell interactions. the method describes (1) the construction of vaccinia viruses recombinant for the major and minor hpv capsid proteins, l1 and l2, respectively, (2) the transfection of cos7 cells with a marker plasmid replicating to high copy number ... | 2005 | 16350418 |
| viral load of episomal and integrated forms of human papillomavirus type 33 in high-grade squamous intraepithelial lesions of the uterine cervix. | the association between total and integrated hpv-33 dna loads and high-grade squamous intraepithelial lesions (hsil) of the uterine cervix was investigated. of 5,347 women recruited in 4 studies, 89 (64 without sil, 7 low-grade sil (lsil), 15 hsil, 3 unknown grade) were infected by hpv-33. hpv-33 e6, hpv-33 e2 and beta-globin dna were measured with real-time pcr that allowed to assess total (e6), episomal (e2) and integrated (e6-e2) hpv-33 viral loads. hpv-33 e6/e2 ratios >/=>/=2.0 suggesting th ... | 2007 | 17708553 |
| production and purification of human papillomavirus type 33 l1 virus-like particles from spodoptera frugiperda 9 cells using two-step column chromatography. | the major capsid protein l1 of human papillomavirus (hpv) is essential in construction of recombinant antigen vaccines against cervical cancer. hpv type 33 accounts for about 10% of all hpv infections in asia. the gene encoding the major capsid protein l1 of the high-risk hpv type 33 was isolated from a korean patient and expressed in sf-9 insect cells using a baculovirus expression system. hpv33 l1 protein was isolated by two-step chromatographic purification using strong-cation exchange and ce ... | 2011 | 20716445 |