| flight behavior and performance of rhodnius pallescens (hemiptera: reduviidae) on a tethered flight mill. | abstract flight dispersal of the triatomine bug species rhodnius pallescens barber, the principal vector of chagas disease in panama, is an important mechanism for spreading trypanosoma cruzi, causative agent of chagas disease. this study measures r. pallescens flight performance using a tethered flight mill both when uninfected, and when infected with t. cruzi or trypanosoma rangeli. forty-four out of the 48 (91.7%) insects initiated flight across all treatments, and trypanosome infection did n ... | 2014 | 25276931 |
| eco-epidemiological aspects of trypanosoma cruzi, trypanosoma rangeli and their vector (rhodnius pallescens) in panama. | the eco-epidemiology of t. cruzi infection was investigated in the eastern border of the panama canal in central panama. between 1999 and 2000, 1110 triatomines were collected: 1050 triatomines (94.6%) from palm trees, 27 (2.4%) from periurban habitats and 33 (3.0%) inside houses. all specimens were identified as r. pallescens. there was no evidence of vector domiciliation. salivary glands from 380 r. pallescens revealed a trypanosome natural infection rate of 7.6%, while rectal ampoule content ... | 2004 | 15361974 |
| human trypanosomiasis in the eastern region of the panama province: new endemic areas for chagas disease. | the epidemiology of chagas disease was studied in five rural communities located in the eastern region of the panama province. serological tests for trypanosoma cruzi infection revealed a prevalence of 5.88% (12/204). hemocultures coupled with polymerase chain reaction (pcr) analysis showed a trypanosoma rangeli infection rate of 5.88% (12/204). an overall trypanosome infection index of 11.76% (24/204) was detected in this population. a total of 121 triatomine specimens were collected in domesti ... | 2010 | 20348502 |
| human trypanosome infection and the presence of intradomicile rhodnius pallescens in the western border of the panama canal, panama. | an entomologic search was carried out to collect intradomicile triatomines in dwellings from rural communities in the western border of the panama canal, panama. sixty-nine triatomines were collected inside 20 houses of 67 houses investigated. rhodnius pallescens was the only triatomine species found and included adults of both sexes and nymphs. a significantly high trypanosoma cruzi (72.7%) and t. rangeli (40%) vector infection rate was detected. blood meal analysis showed that 68% of r. palles ... | 2006 | 16687677 |
| molecular characterisation of trypanosoma rangeli strains isolated from rhodnius ecuadoriensis in peru, r. colombiensis in colombia and r. pallescens in panama, supports a co-evolutionary association between parasites and vectors. | we present data on the molecular characterisation of strains of trypanosoma rangeli isolated from naturally infected rhodnius ecuadoriensis in peru, from rhodnius colombiensis, rhodnius pallescens and rhodnius prolixus in colombia, and from rhodnius pallescens in panama. strain characterisation involved a duplex pcr with s35/s36/kp1l primers. mini-exon gene analysis was also carried out using trint-1/trint-2 oligonucleotides. kdna and mini-exon amplification indicated dimorphism within both dna ... | 2005 | 15639744 |
| prevalence of trypanosome infections in dogs from chagas disease endemic regions in panama, central america. | the prevalence of canine trypanosomosis was investigated in two chagas disease endemic rural communities located in the central region of panama. serologic tests for trypanosoma cruzi infection revealed a prevalence of 11.1%. hemocultures coupled with pcr analysis demonstrated a trypanosoma rangeli infection rate of 5.1%. an overall trypanosome infection index of 16.2% (16/99) was detected in this canine population. one dog had a mixed infection of t. cruzi and t. rangeli. six of the trypanosome ... | 2011 | 21273002 |
| detection of trypanosoma cruzi and t. rangeli infections from rhodnius pallescens bugs by loop-mediated isothermal amplification (lamp). | we have developed two loop-mediated isothermal amplification (lamp) assays for specific detection of trypanosoma cruzi and trypanosoma rangeli based on the 18s ribosomal rna (rrna) and the small nucleolar rna (snorna) genes, respectively. the detection limit of the assays is 100 fg and 1 pg for t. cruzi and t. rangeli, respectively, with reactions conducted in 60 minutes. the two lamp assays were used in detection of t. cruzi and t. rangeli infections in comparison with polymerase chain reaction ... | 2010 | 20439966 |
| trypanosoma rangeli genotypes association with rhodnius prolixus and r. pallescens allopatric distribution in central america. | previous kdna polymorphism-based reports have revealed the existence of two trypanosoma rangeli genotypes (kp1+ and kp1-): sl and ssu rrna gene polymorphism-based studies have revealed that five genotypes (a-e) are distributed throughout different latin-american countries. some evidence has shown that the genotypes' biogeographical distribution is associated with sympatric rhodnius species. 12 t. rangeli isolates from humans and reservoirs from el salvador, guatemala, honduras, costa rica and pa ... | 2009 | 19778637 |
| detection of wolbachia bacteria in multiple organs and feces of the triatomine insect rhodnius pallescens (hemiptera, reduviidae). | at least two types of wolbachia bacteria were detected in wild and insectarium-raised rhodnius pallescens, a natural vector of trypanosoma cruzi and trypanosoma rangeli. wolbachia was detected in all the organs and tissues studied and in the feces, and this provided a methodological advantage for determining the presence of this endosymbiont in this host, obviating the need to kill the specimens. the occurrence of trypanosomatids in wild individuals was also studied. | 2009 | 19028913 |
| feeding sources and trypanosome infection index of rhodnius pallescens in a chagas disease endemic area of amador county, panama. | the sylvatic triatomine rhodnius pallescens is considered to be the most important and widespread vector of trypanosoma cruzi and trypanosoma rangeli in panama. however, its behavior and biological characteristics have only been partially investigated. thus, to achieve sustainable and efficient control over chagas disease in panama, a better understanding of the ecology and biology of r. pallescens is essential. in this study we evaluated r. pallescens host feeding sources using a dot-blot assay ... | 2008 | 18488091 |