| [sensitivity of bordetella bronchiseptica, pasteurella multocida and mycoplasma hyorhinis strains isolated from pigs towards antibiotics and chemotherapeutic agents]. | | 1978 | 100928 |
| relationship of porcine cytomegalovirus and b bronchiseptica to atrophic rhinitis in gnotobiotic piglets. | both porcine cytomegalovirus (pcmv) and bordetella bronchiseptica produced rhinitis and pneumonia when inoculated intranasally into young gnotobiotic pigs. with pcmv the nasal lesions were confirmed to the lamina propria, while bordetella produced atrophy of the turbinate bones and hyperplasia and degeneration of the nasal epithelium. some exacerbation of the lesions was observed in the nasal mucosa of pigs given both agents, but the degree of bone atrophy was not increased. | 1976 | 176765 |
| the recovery of bordetella bronchiseptica strains by means of smears from the nasal cavities of pigs. | | 1979 | 398246 |
| effect of carbadox on growth, feed utilization, and development of nasal turbinate lesions in swine infected with bordetella bronchiseptica. | carbadox in combination with sulfamethazine did not interfere with the activity of sulfamethazine in clearing experimentally induced bordetella bronchiseptica nasal infection. evaluations in three field cases of infectious atrophic rhinitis indicated that carbadox in the feed was as effective as chlortetracycline + sulfamethazine + penicillin for improving growth rate and feed efficiency, as compared with nonmedicated controls, and in reducing the prevalence of lesions of infectious atrophic rhi ... | 1979 | 422462 |
| control of atrophic rhinitis with a bordetella bronchiseptica bacterin. | | 1977 | 587561 |
| bordetella rhinitis in pigs: serum and nasal antibody response to bordetella bacterins. | the nasal and serum antibody response of two groups of pigs, vaccinated with adjuvant containing formalinized or sonicated bordetella bronchiseptica bacterins was compared with the response of a nonvaccinated group. the tube agglutination test was used to determine agglutinin titers. following vaccination, all pigs were challenged intranasally with the vaccine strain of bordetella, after which the nasal bordetella flora of vaccinated and nonvaccinated pigs was investigated. sera and nasal secret ... | 1978 | 647455 |
| experimental infection of gnotobiotic piglets with mycoplasma hyorhinis and bordetella bronchiseptica. | | 1977 | 842196 |
| bacteriological variation among bordetella bronchiseptica isolates from dogs and other species. | bacteriological properties of 50 isolates of bordetella bronchiseptica were compared. phase variation, which involved colonial morphology and its associated characters of hemagglutination, hemolysis, acriflavine agglutination; crystal violet staining, flagellation, and fimbriation, occurred among these isolates. organisms representing the three observed morphotypes did not have different growth rates, nor were any differences in their bacteriological characteristics observed after repeated subcu ... | 1977 | 858784 |
| [bordetella-bronchiseptica pneumonia in the swine]. | bordetella have been frequently detectable from lesioned lung material of swine as well as from the upper respiratory tracts of clinically intact animals. pneumonia, different in extent, was produced in primarily specific pathogen free pigs by intratracheal and intranasal application of eight bordetella isolates. moderate to medium manifestations of focal pneumonia with scattered speckles developed, with severity depending on dosage and mode of infection. histologically, they were characterised ... | 1977 | 901131 |
| effect of vaccination of sows with bordetella bronchiseptica on the incidence of atrophic rhinitis in swine. | vaccination of pregnant sows with bordetella bronchiseptica was performed in an spf herd with clinical atrophic rhinitis. agglutinins against the organism were induced in serum and colostrum by the vaccination. the antibody titres were generally higher in colostrum than in corresponding serum samples. the frequency at slaughter of turbinate atrophy (+, ++, +++) was lower among pigs from vaccinated sows (11%) than in pigs borne by unvaccinated sows (20%). in the control group a lower daily weight ... | 1977 | 905119 |
| [occurrence and drug resistance of bacteria pathogenic to the lungs from autopsy material of swine]. | eight hundred and fifty-four piglets which died or were euthanized due to pneumonia or rhinitis atrophicans, were investigated during the period of 1986-1990. of the animals, 569 showed bronchopneumonia, 218 had pleuritis, pericarditis and peritonitis, 165 had rhinitis atrophicans, 58 pleuropneumonia, and 9 animals had fibrinous pneumonia. pasteurella multocida, haemophilus parasuis, bordetella bronchiseptica, actinobacillus pleuropneumoniae and pasteurella haemolytica were isolated in 59.1%, 29 ... | 1992 | 1481214 |
| inhibitory effect of sulfamonomethoxine on capsule formation of bordetella bronchiseptica. | bordetella bronchiseptica phase i organism possesses a capsule and has the ability to agglutinate with k antiserum, although phase iii organism lacks both. the present study examines the effect of sulfamonomethoxine (smmx) on capsule formation of b. bronchiseptica. i also investigated whether or not the organisms possessed a capsule by bacterial agglutination with k antiserum. three smmx-resistant strains of b. bronchiseptica phase i organisms showed loss of agglutinability with k antiserum by c ... | 1991 | 1789025 |
| drug-susceptibility and isolation of a plasmid in haemophilus (actinobacillus) pleuropneumoniae. | we isolated 56 haemophilus (actinobacillus) pleuropneumoniae strains from the pneumonic porcine lung tissues and tested them for antimicrobial susceptibility. two drug-resistant strains were obtained. one, named kh-265, was resistant to streptomycin (sm) and sulfonamide (sa), and the other, named kh-195, was resistant to tetracycline (tc). the minimum inhibitory concentrations (mics) of drugs for resistant strains were 100 micrograms/mliters for sm, 3200 micrograms/mliters for sa, and 12.5 micro ... | 1990 | 1968988 |
| serum sensitivity and lipopolysaccharide characteristics in bordetella bronchiseptica, b. pertussis and b. parapertussis. | the viability of four strains of bordetella bronchiseptica, two strains of b. pertussis and one strain of b. parapertussis exposed to hyperimmune and pre-colostrum porcine serum was examined. viable cell numbers (cfu/ml) of the b. pertussis strains and a rough strain of b. bronchiseptica (csu-p-1) decreased by 99% and 99.99%, respectively, after exposure for 1 h to porcine hyperimmune serum. in contrast, smooth b. bronchiseptica strains and the b. parapertussis strain showed no significant decre ... | 1991 | 2010907 |
| pneumonia in a sow due to streptococcus suis type ii and bordetella bronchiseptica. | | 1991 | 2063536 |
| identification of a 68-kilodalton outer membrane protein as the major protective antigen of bordetella bronchiseptica by using specific-pathogen-free piglets. | maternal antibody to an outer membrane 68-kilodalton (kda) protein of bordetella bronchiseptica was shown to be protective in experiments on specific-pathogen-free piglets. after challenge with b. bronchiseptica, 100% (n = 19) control piglets from nonimmunized sows developed pneumonia, coughing, and sneezing, and 74% of the animals developed severe atrophic rhinitis. in 12 piglets from a sow immunized with 68-kda protein, pneumonia occurred only in 34% of offspring, coughing was reduced, the dur ... | 1990 | 2137111 |
| differences in virulence between two strains of streptococcus suis type ii after experimentally induced infection of newborn germ-free pigs. | fifteen newborn germ-free pigs were inoculated with 2 strains, d-282 and t-15, of streptococcus suis type ii. some pigs also were preinoculated with bordetella bronchiseptica, which successfully predisposed them to s suis infection. the 2 streptococcal strains were differentiated by muramidase treatment, which released certain high molecular-weight proteins, termed muramidase-released proteins (mrp), from the cell wall of strain d-282, but not from the cell wall of strain t-15. only strain d-282 ... | 1989 | 2774320 |
| bordetella adenylate cyclase: a genus specific protective antigen and virulence factor. | most of the adenylate cyclase (ac) present in bordetella species is localized in the outer membrane, partly exposed to the cell surface. an isolation procedure to obtain the cell-bound enzyme was applied to bordetella bronchiseptica, bordetella pertussis and bordetella parapertussis. passive transfer of b. bronchiseptica anti-ac antibody, either in the form of monoclonal antibody or antibody transferred to offspring from female mice previously immunized with immunopurified b. bronchiseptica ac, ... | 1985 | 2872113 |
| an evaluation in pigs of nobi-vac ar and an experimental atrophic rhinitis vaccine containing p multocida dnt-toxoid and b bronchiseptica. | the trial involved eight large white sows obtained from a closed experimental specific pathogen free herd. four sows (two each for an experimental vaccine and for nobi-vac ar) were vaccinated twice (eight weeks and two weeks before parturition) with 2 ml of vaccine administered intramuscularly. two unvaccinated sows were used as an infected control group and two unvaccinated sows served as an uninfected control group. forty-six piglets (28 from vaccinated sows and 18 from unvaccinated sows) were ... | 1989 | 2919495 |
| evaluation of bordetella bronchiseptica vaccines in specific-pathogen-free piglets with bacterial cell surface antigens in enzyme-linked immunosorbent assay. | the progenies of specific-pathogen-free sows which had been immunized with bordetella bronchiseptica vaccines of various origin before parturition were challenged intranasally with b. bronchiseptica within 5 days of birth. sera of piglets were taken weekly and investigated by enzyme-linked immunosorbent assay against a mixture of b. bronchiseptica cell surface antigens containing curled fibers and fimbriae, lipopolysaccharide, and a mixture of proteins mostly derived from the outer membrane. the ... | 1985 | 2931379 |
| [the differentiation of bordetella bronchiseptica strains. 5. virulence factors in relation to phase changes in bordetella bronchiseptica strains]. | | 1988 | 3219030 |
| bovine erythrocyte-agglutinin as a possible adhesin of bordetella bronchiseptica responsible for binding to porcine nasal epithelium. | the bovine erythrocyte-agglutinin (bea) of bordetella bronchiseptica, located on the cell surface in non-fimbrial form, has been identified as a possible adhesion responsible for binding to porcine nasal epithelium in studies with bea-negative (bea-) mutants and in competitive studies with bovine erythrocytes. | 1988 | 3392727 |
| [the differentiation of bordetella bronchiseptica strains. 7. phenotypic changes in bordetella bronchiseptica through changes of phase]. | | 1988 | 3395180 |
| effect of antigenic modulation and phase variation on adherence of bordetella bronchiseptica to porcine nasal epithelial cells. | in parallel with bacterial density, bordetella bronchiseptica phase-i, x-mode cells adhered well to porcine nasal epithelial cells, whereas c-mode cells and variant cells in phases ii, iii, and rough adhered feebly. cell surface components lost, on transition from x to c mode or from phase i to degraded phases, may confer much of the organism's adherence ability. | 1987 | 3434914 |
| clonal diversity and host distribution in bordetella bronchiseptica. | a total of 303 isolates of bordetella bronchiseptica recovered from 11 host species were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 21 distinctive multilocus genotypes (electrophoretic types) were distinguished on the basis of allele profiles at the enzyme loci. the population structure of b. bronchiseptica is clonal, and its genetic diversity is limited in comparison with most other pathogenic bacteria, perhaps reflecting a relatively recent origin of the speci ... | 1987 | 3584070 |
| the synergistic activity of tiamulin and chlortetracycline: in-feed treatment of bacterially complicated enzootic pneumonia in fattening pigs. | the antibacterial effects of a combination of tiamulin and chlortetracycline in vitro against a number of field isolates of pasteurella multocida, haemophilus pleuropneumoniae and bordetella bronchiseptica were examined. there was a marked synergism between the two antibiotics against all eight isolates of p multocida, against seven of nine isolates of h pleuropneumoniae and against the single strain of b bronchiseptica tested. two field trials were carried out on a herd with a history of compli ... | 1986 | 3750792 |
| [quantitative study of the sensitivity of bordetella bronchiseptica and pasteurella multocida to sulfonamides]. | information concerning the resistance to sulfonamides in the netherlands was obtained by determining the minimal inhibitory concentrations (mic's) of 119 strains of bordetella bronchiseptica and 151 strains of pasteurella multocida, obtained from pigs at five veterinary bacteriology centres, to sulfadimidine (sdm) and sulfamethoxazole (smx). the mic's of sdm against su-susceptible strains were usually 4 times higher than those of smx. about one third of b. bronchiseptica isolates were resistant ... | 1987 | 3824346 |
| bordetella bronchiseptica infections in germ-free swine: an experimental pneumonia. | | 1973 | 4802816 |
| blood chemical changes in growing pigs exposed to aerosol of bordetella bronchiseptica. | | 1974 | 4819726 |
| atrophic rhinitis produced by intranasal inoculation of bordetella bronchiseptica in hysterectomy produced colostrum-deprived pigs. | | 1971 | 5166142 |
| immunogenicity and safety of an attenuated bordetella bronchiseptica vaccine in pigs. | the immunogenicity and safety of an attenuated bordetella bronchiseptica vaccine for swine atrophic rhinitis (ar) was evaluated in 22 hysterectomy-produced, colostrum-deprived pigs and 18 conventional pigs. none of 8 pigs inoculated at 7 days of age intranasally with greater than or equal to 3 x 10(5) colony-forming units (cfu) of vaccinal strain/pig and 2 of 5 pigs inoculated at 7 days of age intranasally with 3 x 10(4) cfu of the vaccinal strain/pig developed ar after intranasal challenge expo ... | 1984 | 6497138 |
| comparative efficacy of sulfamethazine and sulfathiazole in feed for control of bordetella bronchiseptica infection in swine. | seventy-two conventionally raised pigs were challenge exposed intranasally when approximately 3.5 weeks old with yolk-grown bordetella bronchiseptica. twenty-four pigs acted as noninfected, nonmedicated controls. feed containing sulfamethazine or sulfathiazole (110 mg/kg of feed) was initiated in 2 groups of 24 infected pigs each 3 days after challenge exposure and was fed continuously for 56 days. twenty-four infected pigs were given nonmedicated feed. challenge exposure with the b bronchisepti ... | 1984 | 6731985 |
| virulence of pasteurella multocida in atrophic rhinitis of gnotobiotic pigs infected with bordetella bronchiseptica. | bordetella bronchiseptica and pasteurella multocida have been implicated in the aetiology of atrophic rhinitis of pigs but the precise cause and pathogenesis of field outbreaks have still to be clarified. the virulence of 11 strains of p multocida was investigated by intraperitoneal injection of culture filtrates in balb/c mice, or by infection of gnotobiotic piglets given b bronchiseptica five days previously. three of four type d strains of p multocida were lethal for mice and caused severe tu ... | 1983 | 6878879 |
| [bordetella bronchiseptica infection in a spf pig herd. contribution to the aetiology of atrophic rhinitis (a. r.) (author's transl)]. | | 1982 | 7044749 |
| quantitative observations on bordetella bronchiseptica infection in atrophic rhinitis of pigs. | clinical atrophic rhinitis in seven pig herds could not be associated with the infection rate or higher numbers of b bronchiseptica in nasal swabs when compared with unaffected herds. b bronchiseptica isolates from herds with atrophic rhinitis and receiving sulphonamide medication were resistant to sulphonamides in vitro and there was a beneficial clinical response after changing to oxytetracycline medication. in an unaffected herd three piglets naturally infected with b bronchiseptica but posse ... | 1981 | 7292916 |
| detection of specific systemic and local igg and iga antibodies of pigs after infection with bordetella bronchiseptica by elisa. | an enzyme linked immunosorbent assay (elisa) for detection of igg and iga antibodies against bordetella bronchiseptica in serum and nasal secretions of pigs was developed. the elisa that used formolized phase i organisms of b. bronchiseptica as an antigen detected antibodies to a capsular k antigen(s) of the organism. in pigs which had an agglutinin titer of less than 10 and an elisa value of 0.47, on average, of maternal antibodies to b. bronchiseptica, igg antibody in serum increased 4 weeks o ... | 1994 | 8075212 |
| immunogens of pasteurella. | the family pasteurellaceae pohl contains gram-negative, facultatively anaerobic and fermentative bacteria of the genera pasteurella, haemophilus, and actinobacillus. approximately 20 different species of the genus pasteurella have been identified using phenotypic and genetic analyses. of these species, p. multocida and p. haemolytica are the most prominent pathogens in domestic animals causing severe diseases and major economic losses in the cattle, swine, sheep, and poultry industries. mechanis ... | 1993 | 8116191 |
| biochemical and immunological analyses of the cell surface of bordetella bronchiseptica isolates with special reference to atrophic rhinitis in swine. | the guinea-pig skintest was used to test the pathogenic character of nine bordetella bronchiseptica isolates derived from a number of different animals. one isolate was non-pathogenic, one showed a doubtful reaction whereas the others were pathogenic. analysis of the cell envelope protein patterns by sodium dodecyl sulphate polyacrylamide gel electrophoresis revealed only minor differences. two major protein bands and at least thirty five minor bands were observed. the major protein with an appa ... | 1984 | 6531966 |
| interaction between immunity to bordetella bronchiseptica and infection of pig herds by bordetella bronchiseptica and pasteurella multocida. | the dynamics of toxigenic bordetella bronchiseptica and pasteurella multocida infection and the b. bronchiseptica specific antibody content of the blood and nasal secretion were studied in three hungarian and three dutch pig herds. in both countries, the studies involved young sows that had farrowed once or twice (ys), old sows that had farrowed more than four times (os), and their piglets. the results indicate that dutch sows are characterized by a lower prevalence of b. bronchiseptica and p. m ... | 1993 | 8399743 |
| influence of vaccination of sows with bordetella-pasteurella vaccines on the occurrence of atrophic rhinitis among their offspring after experimental infection with bordetella bronchiseptica and toxigenic pasteurella multocida. | experimental infections with bordetella bronchiseptica and a toxigenic strain of pasteurella multocida were carried out in newborn piglets from 25 sows. severe progressive atrophic rhinitis corresponding to the natural disease was produced. the effect of vaccination of sows during pregnancy with two vaccines containing antigens from b. bronchiseptica and toxigenic p. multocida on the incidence of nasal lesions in the offspring was studied. | 1984 | 6531207 |
| drug resistance and r plasmids in bordetella bronchiseptica isolates from pigs. | a total of 207 strains of bordetella bronchiseptica isolated from pigs in 1978 and 1979 were tested for drug resistance and for the properties of their r plasmids. apart from intrinsic resistance to spectinomycin, single (sulfadimethoxine), double (sulfadimethoxine and streptomycin), andt riple (sulfadimethoxine, streptomycin, and ampicillin) resistance were found in 54.1%, 1.0%, and 15.9% of the strains, respectively. all of the triple-resistance determinants were associated with mercury resist ... | 1981 | 6457240 |
| measurement of serum antibody in swine vaccinated with bordetella bronchiseptica: comparison of agglutination and enzyme-linked immunosorbent assay methods. | the immune responses of 514 pigs from 5 swine breeds (chester white, duroc, hampshire, landrace, yorkshire) to vaccination with bordetella bronchiseptica were measured by agglutination and enzyme-linked immunosorbent assay (elisa) methods. both assays showed higher antibody levels in sera of pigs after vaccination than in sera of the pigs before vaccination. the variability of the responses among breeds was greater for the agglutination method than for the elisa method. the elisa proved to be mo ... | 1984 | 6395741 |
| alkaline phosphatase activity in the turbinates of pigs infected with bordetella bronchiseptica. | | 1982 | 6218188 |
| nasal lesions and alcaligenes bronchisepticus infection in swine atrophic rhinitis. | | 1971 | 5133418 |
| a suggested mechanism for the pathogenesis of infectious atrophic rhinitis. | boivin extracts of bordetella bronchiseptica inhibited or uncoupled the energized processes of bovine heart and pig heart mitochondria. energy-dependent accumulation of calcium phosphate by both types of mitochondria was markedly inhibited by the extracts. the thesis is advanced that boivin extracts of b. bronchiseptica may contain the membrane-damaging component responsible for infectious atrophic rhinitis. the action of the extracts was decreased by heat but seemed rather insensitive to digest ... | 1971 | 4260946 |
| [pathohistological research on the white mouse after infection with swine strains of bordetella bronchiseptica. 1. pathohistological research results using intravenously infected mice]. | | 1985 | 3994494 |
| induced atrophic rhinitis in rats. | infections with bordetella bronchiseptica and pasteurella multocida were inducted in newborn specific-pathogen-free rats. turbinate atrophy was quantified by measuring the length of the osseous core of the ventral turbinates. bordetella bronchiseptica readily colonized the nasal cavity. inoculated rats developed serum agglutinating antibodies to b bronchiseptica. turbinate atrophy, correlating with a severe inflammatory reaction, was observed after dual inoculation with b bronchiseptica or after ... | 1986 | 3789506 |
| bordetella bronchiseptica and toxigenic type d pasteurella multocida as agents of severe atrophic rhinitis of swine. | bordetella bronchiseptica and toxigenic type-d pasteurella multocida were cultured from pigs in each of five herds diagnosed as having severe atrophic rhinitis (ar). b. bronchiseptica alone, p. multocida alone, or both organisms isolated from four herds were inoculated intranasally into 1-week-old gnotobiotic pigs which were necropsied 4 weeks post-inoculation (pi). nasal turbinate atrophy in b. bronchiseptica-inoculated pigs was moderate to severe, while p. multocida-inoculated pigs had slight ... | 1987 | 3564360 |
| restriction endonuclease analysis discriminates bordetella bronchiseptica isolates. | one hundred ninety-five bordetella bronchiseptica isolates from 12 different host species worldwide were characterized by restriction enzyme analysis (rea). these isolates had previously been categorized into 19 pvuii ribotypes. twenty restriction endonucleases were evaluated for use in rea. digestion of chromosomal dna with hinfi, followed by submarine electrophoresis in agarose gels and staining with ethidium bromide, produced dna fragments in the 4.0- to 10-kb range, which readily discriminat ... | 2000 | 11101569 |
| development of turbinate lesions and nasal colonization by bordetella bronchiseptica and pasteurella multocida during long-term exposure of healthy pigs to pigs affected by atrophic rhinitis. | natural transmission of atrophic rhinitis from pigs from a herd with an endemic atrophic rhinitis problem to pigs from a herd free of atrophic rhinitis was demonstrated. six replicates each with five pigs from the endemic atrophic rhinitis herd (group a) and five pigs from the atrophic rhinitis-free herd (group b) were housed together from 5 wk of age, with each replicate kept in isolation rooms maintained at optimal and controlled environmental conditions. three replicates each with six pigs/ro ... | 1988 | 3349398 |
| evaluation of vaccines for atrophic rhinitis--a comparison of three challenge models. | we compared three challenge models for the assessment of atrophic rhinitis (ar) vaccines: combined infection with bordetella bronchiseptica (bb) and pasteurella multocida (pm); application of acetic acid (aa) to the nasal mucosa followed by pm infection; and bb infection alone. two vaccines were tested using standardized criteria, notably nasal lesion scores. the vaccines provided different levels of protection in the bb and the aa/pm challenges, but were similar in the combined (bb/pm) challeng ... | 2002 | 11906767 |
| mucosal immunoadjuvant activity of the low toxic recombinant escherichia coli heat-labile enterotoxin produced by bacillus brevis for the bacterial subunit or component vaccine in pigs and cattle. | a gene encoding the mature escherichia coli heat-labile enterotoxin (lt) lacking the nick site in the a subunit by deleting tripeptides was introduced in a vector pnh301 and expressed extracellularly as mutant molecule of holotoxin at high levels in bacilus brevis hpd31-s5 of the host bacterium. the mucosal adjuvant activities of the produced mutant lt (mlt) preparation were studied in pigs and cattle. intranasal immunization of pigs with the recombinant subunit vaccine of erysipelothrix rhusiop ... | 2002 | 12072249 |
| distribution of fim3 and flaa ttge sequence types amongst isolates of bordetella bronchiseptica from different host animals. | isolates of bordetella bronchiseptica associated with different animal hosts were analysed by fim3 and flaa genotyping by temporal temperature gradient gel electrophoresis (ttge). all the isolates from cats (n = 138), dogs (n = 42) and pigs (n = 13) could be assigned to one of two fim3 and one of three flaa ttge sequence types, respectively. two avian isolates and a marmoset isolate exhibited novel fim3 sequence types. dominant but different ttge sequence types were apparent in isolates from dog ... | 2002 | 12132771 |
| plasmids related to rsf1010 from bordetella bronchiseptica. | six out of 14 bordetella bronchiseptica isolates from u.k. pigs each contained one plasmid, of 8.7-44 kb. all plasmid-containing isolates were sulfonamide resistant, and this property was shown to be plasmid-encoded. five of the plasmids were related; two were indistinguishable from the broad-host-range plasmid, rsf1010. the other three, two of which appeared to be identical, were shown to have regions of homology with rsf1010. one of these regions encompassed the sulfonamide resistance determin ... | 1986 | 3012614 |
| efficacy of a commercial bacterin in protecting strain 13 guineapigs against bordetella bronchiseptica pneumonia. | bordetella bronchiseptica is known to be endemic in many guineapig (cavia porcellus) colonies, and periodically is the aetiological agent of fatal epizootics of bronchopneumonia. a commercial, non-adjuvant b. bronchiseptica bacterin, which is approved for use in canines, was evaluated for induction of a protective immune response in strain 13/n guineapigs against an airborne challenge of virulent b. bronchispeptica in small-particle aerosol. seronegative animals were vaccinated on days 0 and 21 ... | 1989 | 2761230 |
| [atrophic rhinitis of swine--a multifactorial disease?]. | atrophic rhinitis is caused by toxigenic pasteurella multocida strains. the infection is supported by bordetella bronchiseptica and other widespread agents and by non-infectious factors damaging the nasal epithelium. the term "infectious multifactorial disease" means in german, that ubiquitous low-virulent agents cause disease when intensified by non-infectious factors. atrophic rhinitis does not belong to this group but is an infectious disease, caused by a specific agent and influenced by seve ... | 1989 | 2686620 |
| protection against progressive atrophic rhinitis by vaccination with pasteurella multocida toxin purified by monoclonal antibodies. | pasteurella multocida toxin was purified by affinity chromatography and inactivated by treatment with formaldehyde before use as a single component vaccine against progressive atrophic rhinitis in pigs. twenty pregnant gilts which were vaccinated twice before farrowing with either low or high doses of the purified toxoid, developed dose-dependent positive serum and colostrum titres to the toxin and, unlike the progeny of 10 untreated control gilts, the offspring of the vaccinated gilts also had ... | 1989 | 2528856 |
| pneumonia of pigs: a review. | pneumonia of pigs is one of the more important disease factors limiting pig production. of the varieties of pneumonia affecting this species enzootic pneumonia caused by mycoplasma spp. is the most common and most important. the major effects of this disease are lowered food conversion ratio and poor weight gain. deaths are usually the result of secondary infection by necrotising, pus-forming bacteria. eradication of the disease is expensive and requires depopulation and restocking. control and ... | 1981 | 16030803 |
| mechanisms of infection in the respiratory tract. | related to its potential vulnerability the respiratory tract has a very complex and effective defence apparatus. the interaction between these defence mechanisms and certain characteristics of aetiological agents results in a pattern in which initial infections by these agents tend to occur at specific sites in the tract. infections in which the primary portal of entry is in the upper respiratory tract include bordetella bronchiseptica and haemophilus spp in pigs; pasteurella spp in cattle, shee ... | 1981 | 16030806 |
| adherence of bordetella bronchiseptica 276 to porcine trachea maintained in organ culture. | two organ culture models have been adapted for porcine tracheae in order to study colonization by bordetella bronchiseptica. rings or segments excised from tracheae of newborn piglets were incubated overnight at 37 degrees c in a nutrient medium under 5% co2-95% air conditions. tracheal segments were infected with b bronchiseptica 276, and after different incubation times, bacterial counts were done. b. bronchiseptica adhered well to tracheae maintained in culture, and no statistically significa ... | 1990 | 2383001 |
| pk and pk/pd of doxycycline in drinking water after therapeutic use in pigs. | a commercial doxycycline formulation was administered in drinking water to 12 pigs at the recommended dose of 10 mg/kg daily for 5 days. the mean plasma concentration at steady-state was 1.37 +/- 1.21 microg/ml, which was reached at 68 +/- 27.2 h postadministration. absorption and elimination half-life values were 7.20 +/- 2.42 and 7.01 +/- 2.10 h, respectively. most plasma concentrations during dosing were higher than the minimum inhibitory concentrations (mics) described for the main porcine b ... | 2005 | 16343284 |
| prevalence of bordetella bronchiseptica in certain central iowa. | bordetella bronchiseptica was isolated from 6 of 13 short-tailed shrews (blarina brevicauda) and 1 of 47 house sparrows (passer domesticus) trapped in the vicinity of a swine bordetella rhinitis experimental area. the organism was found in four of 50 foxes (vulpes fulva), 2 of 36 opossums (didelphis marsupialis) and 1 of 37 raccoons (procyon lotor) trapped in the ames, iowa area. this bacterium was not culturally isolated from 14 deer mice (peromyscus maniculatus), 64 house mice (mus musculus), ... | 1976 | 16502690 |
| the host defense peptide beta-defensin 1 confers protection against bordetella pertussis in newborn piglets. | innate immunity plays an important role in protection against respiratory infections in humans and animals. host defense peptides such as beta-defensins represent major components of innate immunity. we recently developed a novel porcine model of pertussis, an important respiratory disease of young children and infants worldwide. here, we investigated the role of porcine beta-defensin 1 (pbd-1), a porcine defensin homologue of human beta-defensin 2, in conferring protection against respiratory i ... | 2006 | 16552064 |
| tet(a)-mediated tetracycline resistance in porcine bordetella bronchiseptica isolates is based on plasmid-borne tn1721 relics. | | 2006 | 16636083 |
| domestic animals as carriers of bordetella species in senegal. | despite intense efforts to maintain a high level of vaccine coverage against human whooping cough, rural senegalese areas are still endemic for bordetella pertussis. one explanation being the potential existence of animal reservoirs, the objective of this work was to precise the carriage by domestic animals of bacteria belonging to the genus bordetella in senegal. bacteriological samples (swabs and aspirates) were obtained from various domestic animals living in different parts of the country. n ... | 2006 | 16796515 |
| hydrophobic surface properties of bordetella bronchiseptica x-mode cells and their possible role in adherence to porcine nasal mucosa. | bordetella bronchiseptica strains were examined to determine whether the surface hydrophobicity of the bacteria correlates with their adherence to porcine nasal epithelial cells. the relative hydrophobicity of the bacteria, measured by their retention to a column of hydrophobic phenyl-sepharose gel and their aggregation in ammonium sulfate solution, correlated well with their adhesive properties. phase i cells in x-mode, which adhered well to the epithelial cells, were conspicuously hydrophobic. ... | 1990 | 2357669 |
| pluronic f127 enhances the effect as an adjuvant of chitosan microspheres in the intranasal delivery of bordetella bronchiseptica antigens containing dermonecrotoxin. | we have studied a vaccine delivery system in vitro and in vivo based on chitosan microspheres (cms) prepared in the presence of selected immunomodulators, pluronic block copolymer f127 (f127). the bordetella bronchiseptica multiple antigens containing dermonecrotoxin (bbd), a virulent factor leading to atrophic rhinitis (ar) in swine was loaded in cms/f127 or cms alone. the microspheres, prepared using an ionic gelation process with tripolyphosphate, demonstrated release profiles that showed a g ... | 2007 | 17485148 |
| adherence of bordetella bronchiseptica and pasteurella multocida to swine nasal ciliated epithelial cells in vitro. | a bacterial adherence assay using swine nasal turbinate fragments was established. turbinate fragments were incubated with bordetella bronchiseptica or pasteurella multocida type d at different concentrations or for different incubation times at 37 degrees c on a shaker at 120 rev/min. b. bronchiseptica phase i strains exhibited strong adherence to swine nasal ciliated epithelial cells. the number of adherent bacteria per cell increased when the bacterial concentration or incubation time increas ... | 1990 | 2357345 |
| virulence and lienotoxicity of bordetella bronchiseptica in mice. | whole-cell suspensions (wcss) and cell-free sonicated extracts (ses) of seven bordetella bronchiseptica strains were studied for lethality and lienotoxicity in mice. lethality was assessed after intravenous and intracerebral inoculation, and lienotoxicity by splenic atrophy after intravenous inoculation. the strains represented phase i isolates with or without cytotoxin production, their phase iii subcultures and a phase iv variant. the lethality and lienotoxicity of the ses were in close positi ... | 1990 | 2281605 |
| swine infectious agents in tayassu pecari and pecari tajacu tissue samples from brazil. | peccaries and pigs, tayassuidae and suidae respectively, diverged approximately one million years ago from a common ancestor. because these families share some pathogens, peccaries can act as reservoirs of infectious pathogens for domestic and wild swine. we evaluated the presence of swine infectious agents in the spleen and lung tissues of white-lipped peccaries (wlp; tayassu pecari) and collared peccaries (cp; pecari tajacu) in brazil. samples from 10 adult cp and three wlp, which had been hun ... | 2014 | 24484498 |
| distribution of atrophy in the nasal ventral conchae of piglets infected experimentally with bordetella bronchiseptica. | the distribution and extent of atrophy caused by bordetella bronchiseptica in the nasal ventral conchae were investigated by intranasally infecting piglets at day 4 of life. experimental and control piglets were killed at one, two, three, four, five and six weeks of age. serial transverse 80 microns thick nondecalcified sections were cut throughout the entire length of the nasal ventral conchae, and microradiographed. it was observed that the distribution and extent of atrophy was not uniform al ... | 1991 | 1889033 |
| [characterization of strains of bordetella bronchiseptica isolated from animal housing air and of the airborne infection pressure proceeding from them]. | strains of b. bronchiseptica were isolated from the air in farrowing and weaned piglet houses within the framework of follow-up investigations in an intensive pig breeding and fattening farm with 2600 productive sows. the strains were tested for their biological properties. the results show that virulent b. bronchiseptica-strains have been present as an aerosol in the air of both husbandry systems which refers to the infectious pressure of the animal house air. the number of isolated strains of ... | 1991 | 1790763 |
| recombinant porcine somatotropin: an immunotoxicology study. | the present study was designed to determine the effect of recombinant porcine somatotropin (rpst; 5, 15, or 25 mg.pig-1.d-1 for 57 d) on a variety of immune function variables. we observed no significant effect of rpst treatment on the gross pathology of the pigs, histopathology of the immune system organs, total and differential white blood cell counts, lymphocyte blastogenic response to mitogens, or the neutrophil functions of chemotaxis, ingestion, reduction of cytochrome c, iodination, and a ... | 1991 | 1752827 |
| an enzyme-linked immunosorbent assay (elisa) for monitoring guineapigs and rabbits for bordetella bronchiseptica antibodies. | an enzyme-linked immunosorbent assay (elisa) for monitoring antibodies specific to bordetella bronchiseptica in guineapigs and rabbits was developed. in conventional and spf colonies of guineapigs and rabbits, the elisa was equally successful in detecting infected animals when compared to selective cultivation from the respiratory tract. the elisa showed a sensitivity of 100% and a specificity of 90% in guineapigs. in rabbits the sensitivity and specificity of the elisa were 97% and 91% respecti ... | 1993 | 8277707 |
| purification and characterisation of haemagglutinin from bordetella bronchiseptica. | a surface protein of bordetella bronchiseptica was purified in one step by affinity chromatography with bovine submaxillary mucin coupled to agarose. the purified protein, with a mol. wt of 200 kda and an iso-electric point of pi 6.5, showed haemagglutinating activity for bovine erythrocytes. this haemagglutinin (ha) inhibited the adherence of b. bronchiseptica to a rat lung cell line (l2) and was able to bind to n-acetylneuraminic acid. these findings suggest that the ha of b. bronchiseptica is ... | 1993 | 8246255 |
| non-conjugative r plasmid with five drug resistance from bordetella bronchiseptica of pig origin. | | 1981 | 7343701 |
| the quantitation of turbinate atrophy in pigs to measure the severity of induced atrophic rhinitis. | the two-fold purpose of this study was to establish a useful image analysis technique for quantitation of turbinate atrophy and to determine an optimum bacterial dose for inducing atrophic rhinitis (ar). two morphometric analysis methods were compared to determine a turbinate area ratio (tar) and a turbinate perimeter ratio (tpr); the ratios of turbinate area to total nostril area and of turbinate perimeter to total nostril perimeter, respectively. our first image analysis method differed from c ... | 1996 | 8785717 |
| evaluation of a pcr for detection of actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils. | a pcr for the detection of actinobacillus pleuropneumoniae was evaluated. all of 102 field, isolates of a, pleuropneumoniae reacted in the pcr by amplification of a 985 bp product. no pcr amplification product was observed when examining strains of a. ureae, a. capsulatus, a. hominis, a. equuli, a, rossii, a. suis, escherichia coli, bordetella bronchiseptica. streptococcus suis, pasteurella haemolytica, pasteurella multocida, haemophilus parasuis, haemophilus taxon minor group, haemophilus taxon ... | 1996 | 8828126 |
| synergistic role of gaseous ammonia in etiology of pasteurella multocida-induced atrophic rhinitis in swine. | one-week-old large white piglets were weaned and allocated to 14 experimental groups, each composed of five animals. each group was housed in a separate rochester exposure chamber and exposed continuously to gaseous ammonia at either 0, 5, 10, 15, 25, 35, or 50 ppm (two groups per exposure level). one week after ammonia exposure commenced, the pigs from one group at each exposure level were inoculated intranasally with 9 x 10(7) cfu of pasteurella multocida type d. after a further 4 weeks of exp ... | 1996 | 8862582 |
| interpretive criteria for antimicrobial susceptibility testing of ceftiofur against bacteria associated with swine respiratory disease. | ceftiofur, an extended-spectrum cephalosporin, is active against a variety of animal pathogens, including organisms associated with swine respiratory disease. however, minimum inhibitory concentration (mic) breakpoint and disk diffusion interpretive criteria have not been established for swine pathogens. susceptibility tests were performed by broth microdilution mic and disk diffusion methods on 246 bacterial species that cause swine respiratory disease. ceftiofur was active against salmonella s ... | 1996 | 8953533 |
| [bronchoalveolar lavage on pig breeding farms]. | in 182 pigs lung lavage was performed using a endotracheal tube and a catheter. the collected bronchoalveolar lavage fluid (balf) was examined microbiologically. with decreasing numbers alpha-hämolytic streptococci, bordetella bronchiseptica, haemophilus parasuis, pasteurella multocida were cultured. actinobacillus pleuropneumoniae was isolated from 3 balfs. in one farm piglets were lavaged routinely for monitoring of the lung health status. | 1997 | 9410726 |
| [occurrence of bacterial infectious agents in pathologically/anatomically altered lungs of pigs and compilation of resistance spectra]. | the goal of this work was to investigate the type and etiology of bacterial porcine pneumonia by analyses of autopsy findings made in the tierärztlichen ambulanz schwarzenbek, aussenstelle der freien universität berlin, and in the institut für tiergesundheit, milchhygiene und lebensmittelqualität der landwirtschaftskammer westfalen-lippe in münster in the years from 1991 to 1993. the evaluation of the results of the total 6560 autopsies (n = 6560) of pigs shows: 1. pneumonia as the main diagnosi ... | 1998 | 9639952 |
| histochemical and lectinhistochemical studies on nasal mucosa of pigs with or without respiratory diseases. | histochemical and lectinhistochemical examinations were carried out on nasal mucosa of pigs with or without respiratory diseases. as the results, both acid and neutral mucins coexisted in nasal mucosa of normal pigs while acid sialomucins were mainly observed in nasal mucosa of pigs infected with bordetella bronchiseptica and/or pasteurella multocida. lectinhistochemistry revealed that the nasal epithelial cells of normal pigs were rich in n-acetylgalactosamine, fucose and n-acetyl-glucosamine r ... | 1998 | 9795904 |
| biological activities of lipopolysaccharides extracted from porcine vaccine strains. | lipopolysaccharides (lpss) were purified from actinobacillus pleuropneumoniae serotype 2, bordetella bronchiseptica and haemophilus parasuis serotype 5, which were used for vaccine production in japan, by the phenol-water procedure. in sds-page analysis, a. pleuropneumoniae lps, as well as escherichia coli lps, demonstrated a typical ladder profile of a smooth-type lps. on the other hand, b. bronchiseptica and h. parasuis lpss lacked the ladder profiles. it was found that the biological activity ... | 1999 | 10651044 |
| effect of temperature modulation and bvg mutation of bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages. | bordetella bronchiseptica causes respiratory disease in swine, yet there are no studies examining the interaction of b. bronchiseptica with swine alveolar macrophages. a swine isolate of b. bronchiseptica was able to adhere to, and survive intracellularly in, swine alveolar macrophages, but the relative ability of the bacteria to accomplish these functions was dependent on its phenotypic phase and culture conditions. more bacteria were observed extracellularly as well as intracellularly by immun ... | 2000 | 10731613 |
| in vitro susceptibility of porcine respiratory pathogens to tilmicosin. | bacterial isolates obtained from swine with various clinical diseases were tested for susceptibility to tilmicosin by minimum inhibitory concentration (mic) and kirby-bauer disk diffusion tests using national committee on clinical laboratory standards methodology. the tilmicosin mic90 was < or =0.125 microg/ml for erysiopelothrix rhusiopathiae, < or = 1 microg/ml for haemophilus parasuis isolates, 8 microg/ml for actinobacillus suis and pasteurella multocida type a, 16 microg/ml for toxigenic an ... | 2000 | 11108454 |
| mac-1-mediated uptake and killing of bordetella bronchiseptica by porcine alveolar macrophages. | the role of mac-1 as a receptor for bordetella bronchiseptica infection of alveolar macrophages (amphi) was examined using 6 strains (2 atcc and 4 pathogenic field isolates) to assess b. bronchiseptica binding, uptake and replication in primary porcine amphi. all b. bronchiseptica strains were rapidly killed by porcine serum in a dose- and time-dependent manner. however, heat-inactivated porcine serum (his) did not demonstrate any bacterial-killing activity, suggesting that complement may have a ... | 2003 | 12819364 |
| expression of a truncated pasteurella multocida toxin antigen in bordetella bronchiseptica. | mild or subclinical respiratory infections caused by bordetella bronchiseptica are widespread in pigs despite multiple control efforts. infection with virulent b. bronchiseptica strains is a common risk factor in the establishment of toxin-producing strains of pasteurella multocida in the nasal cavity of pigs leading to the disease, atrophic rhinitis (ar). this study was designed to explore the possibility of expressing a protective epitope of p. multocida toxin (pmt) in b. bronchiseptica to cre ... | 2003 | 12829385 |
| controlled release of bordetella bronchiseptica dermonecrotoxin (bbd) vaccine from bbd-loaded chitosan microspheres in vitro. | chitosan microspheres were prepared by ionic gelation process with sodium sulfate for nasal vaccine delivery. bordetella bronchiseptica dermonecrotoxin (bbd) as a major virulence factor of a causative agent of atrophic rhinitis (ar) was loaded to the chitosan microspheres for vaccination. morphology of bbd-loaded chitosan microspheres was observed as spherical shapes. the average particle sizes of the bbd-loaded chitosan microspheres were about 2.69 microm. more bbd was released with an increase ... | 2004 | 15089042 |
| in vitro study of the immune stimulating activity of an atrophic [correction of athrophic] rhinitis vaccine associated to chitosan microspheres. | chitosan microspheres (cms) were prepared by an ionic gelation process with tripolyphosphate and characterized. bordetella bronchiseptica dermonecrotoxin (bbd), a major virulence factor of a causative agent of atrophic rhinitis (ar), was loaded on to the cms for nasal vaccination. bbd-loaded cms were observed as aggregated shapes although unloaded cms were observed as relatively spherical ones. the average particle size of the bbd-loaded cms was 4.39 microm. the lower the molecular weight of chi ... | 2004 | 15451520 |
| prevalence and in-vitro antimicrobial sensitivity of bordetella bronchiseptica in the nasal cavity of pigs. | the prevalence of bordetella bronchiseptica in the nasal cavity of pigs and the in-vitro sensitivity of isolates to a variety of antimicrobial agents was investigated. b. bronchiseptica was recovered from 372 nasal swabs collected from 1000 (37.2%) pigs slaughtered at 20-30 weeks old at an abattoir. the swabs were collected from groups of 5-206 pigs derived from 25 herds. all isolates tested against bacitracin, clindamycin, furazolidone, penicillin, spectinomycin, streptomycin and tylosin were f ... | 1984 | 16031068 |
| diversity of swine bordetella bronchiseptica isolates evaluated by rapd analysis and pfge. | the degree of genetic diversity in 45 bordetella (b.) bronchiseptica strains comprised of a vaccine strain (n = 1), reference strains (n = 3) and field isolates (n = 41) was evaluated using random amplified polymorphic dna (rapd) fingerprinting and pulsed-field gel electrophoresis (pfge). three candidate primers were selected for rapd analysis after screening 20 random decamer oligonucleotides for their discriminatory abilities. the opa-07, opa-08 and opa-18 primers yielded 10, 10, and 6 distinc ... | 2007 | 17322776 |
| contribution of bordetella bronchiseptica filamentous hemagglutinin and pertactin to respiratory disease in swine. | bordetella bronchiseptica is pervasive in swine populations and plays multiple roles in respiratory disease. most studies addressing virulence factors of b. bronchiseptica are based on isolates derived from hosts other than pigs. two well-studied virulence factors implicated in the adhesion process are filamentous hemagglutinin (fha) and pertactin (prn). we hypothesized that both fha and prn would serve critical roles in the adhesion process and be necessary for colonization of the swine respira ... | 2009 | 19237531 |
| immune-enhancing effect of fermented maesil (prunus mume siebold & zucc.) with probiotics against bordetella bronchiseptica in mice. | maesil (prunus mume) has long been used as a traditional drug and healthy food in east asian countries. it possesses a number of beneficial biological activities including potential antimicrobial effects against pathogens. probiotics also have antibacterial effects. moreover, some probiotics have an important role in regulating the immune system. the present study evaluated the immune enhancing effects of fermented maesil with probiotics (saccharomyces cerevisiae, bacillus subtilis and lactobaci ... | 2010 | 20453453 |