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comparative analysis of the complete genome of an acinetobacter calcoaceticus strain adapted to a phenol-polluted environment.the complete genome sequence of acinetobacter calcoaceticus phea-2, a non-pathogenic phenol-degrading bacterium previously isolated from industrial wastewater of an oil refinery in china, has been established. this is the first sequence of an a. calcoaceticus strain. we report here a comparative genomic analysis of phea-2 with two other acinetobacter species having different lifestyles, acinetobacter baumannii aye, a pathogenic human-adapted strain, and acinetobacter baylyi adp1, a soil-living s ...201122027104
pathophysiology of surgical site infection in total hip arthroplasty.this article is a case report of a 69-year-old man who underwent a right total hip replacement procedure and developed a surgical site infection. areas of concern in prevention and treatment of hip arthroplasty infection are presented, focusing on the pathophysiologic process involved. a review of the patient risk factors and the pathophysiologic action potentiating risk for infection include host immunity, nutritional status, diabetes, age, use of steroids or immunosuppressive drugs, rheumatoid ...199910586159
distribution of acinetobacter species on skin of healthy humans.the distribution of the 19 currently known genospecies of acinetobacter on human skin, i.e. forehead, forearm and toe webs, was determined. three selective media were compared for their specificity for all genospecies of acinetobacter. a minimal-salts agar supplemented with 1% acetate proved to be more efficient than the leeds medium for the isolation of most genospecies in mixed culture with other bacterial species. acinetobacter isolates were provisionally identified using biochemical tests an ...199910357050
immune response to acinetobacter calcoaceticus infection in man.after growth in an iron-depleted chemically-defined medium acinetobacter calcoaceticus expressed four high mol. wt outer-membrane proteins (omps) which were repressed under iron supplementation or in a complex laboratory medium. immunoblotting with serum from a septicaemic patient infected with a. calcoaceticus revealed antibody binding to these iron-repressible omps, indicating that they were expressed in vivo, and also to the 42- and 18-kda omps. although the antibody response to the omps did ...19911990144
leprosy in hiv-positive and syphilitic young paraguayan man.in a borderline lepromatous patient a positive serology for syphilis and hiv 1 has been detected. the patient also had an urinary tract infection with acinetobacter calcoaceticus. in paraguay up till now no leprosy cases infected with hiv have been reported.19921293911
antibacterial activity in spices and local medicinal plants against clinical isolates of karachi, pakistan.context: development of resistance in human pathogens against conventional antibiotic necessitates searching indigenous medicinal plants having antibacterial property. twenty-seven medicinal plants used actively in folklore, ayurvedic and traditional system of medicine were selected for the evaluation of their antimicrobial activity for this study. eleven plants chosen from these 27 are used as spices in local cuisine. objective: evaluation of the effectiveness of some medicinal plant extracts a ...201121501041
aerobic bacterial oral flora of garter snakes: development of normal flora and pathogenic potential for snakes and humans.garter snakes that are used for scientific laboratory studies or kept as exotic pets often become ill and die early in captivity. they may also act as reservoirs of potential human pathogens or transmit infection to man. a total of 126 strains of aerobic and facultative bacteria, most potential human and snake pathogens, were isolated from 82 garter snake oropharyngeal cultures. coagulase-negative staphylococcus species were the most common species isolated. acinetobacter calcoaceticus var. anit ...19817240404
cloning and expression of dipeptidase from acinetobacter calcoaceticus atcc 23055.the gene encoding dipeptidase was cloned from acinetobacter calcoaceticus atc 23055. determination of the nucleotide sequence revealed that the gene had an open reading flame of 1,050 bp coding a protein of 350 amino acids. the deduced amino acid sequence showed 48.8% similarity to human renal dipeptidases and conserved two amino acid residues identified in human and pig renal dipeptidases as essential ones for the catalytic activity. purified recombinant enzyme expressed in escherichia coli did ...19958690717
nk-lysin, a novel effector peptide of cytotoxic t and nk cells. structure and cdna cloning of the porcine form, induction by interleukin 2, antibacterial and antitumour activity.a 78 residue antimicrobial, basic peptide, nk-lysin, with three intrachain disulfide bonds was purified from pig small intestine and characterized. a corresponding clone was isolated from a porcine bone marrow cdna library. the 780 bp dna sequence had a reading frame of 129 amino acids which corresponded to nk-lysin. the clone was used to show that stimulation with human interleukin-2 induced synthesis of nk-lysin-specific mrna in a lymphocyte fraction enriched for t and nk cells. lower levels o ...19957737114
adjuvant activity of emulsan, a secreted lipopolysaccharide from acinetobacter calcoaceticus.several promising adjuvant candidates have been studied over the past 75 years; however, only alum is currently approved for human use. the complex acylated polysaccharide emulsan, secreted from acinetobacter calcoaceticus, represents a new candidate. unique features of this family of polymers are their amenability to structural tailoring and their emulsification behavior. we demonstrate that emulsan activates macrophages in a dose-dependent manner. this activation is dependent on the presence o ...200212414756
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