regions on plasmid pcu1 required for the killing of klebsiella pneumoniae.plasmid pcu1 was kik+ (promotes killing of klebsiella pneumoniae). all tn5 insertions within the tra region of pcu1 were kik-. two other regions, kika and kikb, were needed. they may be separated on different plasmids, but both must be mobilized into klebsiella pneumoniae. establishment of one kik region in k. pneumoniae followed by receipt of the second did not lead to killing. kik was therefore intracellular and required concerted and transient action of both regions.19852993244
structure and mode of action of kika, a genetic region lethal to klebsiella oxytoca and associated with conjugative antibiotic-resistance plasmids of the incn group.transmission of conjugative plasmids of the incn group into klebsiella oxytoca, but not into escherichia coli, results in the marked reduction of viability of the recipients. in the plasmid pcu1 a 500-bp locus called kika has the major role in determining this phenotype. expression of two open reading frames (orf104 and orf70) is required for the kik+ phenotype and they can function in cis or in trans. orf104 encodes a 8.9-kda soluble protein which is translocated into the periplasm. orf70 encod ...19968812785
determination of the binding sites of repa, a replication initiator protein of the basic replicon of the incn group plasmid pcu1.a 2kb dna region of the broad-host-range plasmid pcu1 carries all of the information essential for the stable maintenance of the plasmid and to express the same host-range specificity. it was predicted that the protein required to initiate replication from at least one of the three origins of the plasmid is encoded by the longest open-reading frame (orf239) of the three overlapping in-frame orfs located within the 2 kb region. the product of orf239 has been named repa. the initiator protein was ...19957877179
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