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cloning and dna sequence of plasmid determinant iss, coding for increased serum survival and surface exclusion, which has homology with lambda dna.escherichia coli k12 cells carrying a cloned 1.4 kb hindiii fragment from plasmid colv2-k94, showed increased survival in guinea pig serum. the recombinant plasmid also conferred group ii surface exclusion, i.e. the cells were reduced in recipient ability towards the incoming plasmid r538drd in conjugation experiments. southern blotting suggested homology with bacteriophage lambda dna and to the insertion element is2. determination of the dna sequence of the fragment demonstrated the presence of ...19892546040
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