| the traa relaxase autoregulates the putative type iv secretion-like system encoded by the broad-host-range streptococcus agalactiae plasmid pip501. | the conjugative multiple antibiotic resistance plasmid pip501 can be transferred and stably maintained in a variety of gram-positive genera, including multicellular streptomyces lividans, as well as in gram-negative escherichia coli. the 15 putative pip501 transfer (tra) genes are organized in an operon-like structure terminating in a strong transcriptional terminator. this paper reports co-transcription of the pip501 tra genes in exponentially growing enterococcus faecalis jh2-2 cells, as shown ... | 2006 | 16514144 |
| sequence of the 50-kb conjugative multiresistance plasmid pre25 from enterococcus faecalis re25. | the complete 50,237-bp dna sequence of the conjugative and mobilizing multiresistance plasmid pre25 from enterococcus faecalis re25 was determined. the plasmid had 58 putative open reading frames, 5 of which encode resistance to 12 antimicrobials. chloramphenicol acetyltransferase and the 23s rna methylase are identical to gene products of the broad-host-range plasmid pip501 from streptococcus agalactiae. in addition, a 30.5-kb segment is almost identical to pip501. genes encoding an aminoglycos ... | 2001 | 11735367 |
| plasmid pip501 encoded transcriptional repressor copr binds asymmetrically at two consecutive major grooves of the dna. | replication of the streptococcal plasmid pip501 is regulated by the copr protein and an antisense-rna (rnaiii). copr acts as transcriptional repressor at the essential repr promoter pii by binding to inverted repeat ir1 upstream of pii. to further characterize the interaction of copr with its target, footprinting studies were performed. methylation interference identified three guanine bases (g240, g242 and g251) in the top strand and two (g252 and g254) in the bottom strand contacted by copr in ... | 1997 | 9223633 |
| chromosomal gentamicin resistance transposon tn3706 in streptococcus agalactiae b128. | streptococcus agalactiae b128 is the only highly gentamicin-resistant group b streptococcal (gbs) strain described so far. this strain carries a chromosomal gentamicin resistance transposon, designated tn3706, which is similar, if not identical, to the tn4001 and tn5281 transpons detected in staphylococcus aureus and enterococcus faecalis, respectively. transposition of tn3706 occurred onto the gbs plasmid pip501 in two different loci of its 7.5-kb avaii fragment carrying the genes for chloramph ... | 1996 | 8723445 |
| location of antibiotic resistance markers in clinical isolates of enterococcus faecalis with similar antibiotypes. | eight wild-type strains of enterococcus faecalis, resistant to chloramphenicol (cmr), erythromycin (emr), tetracycline (tcr), and minocycline (mnr), were examined for the genetic basis of their antibiotic resistance, five of the strains transferred all of their antibiotic resistance markers by conjugation, while the other three strains transferred only tcr and mnr. cmr and emr determinants were localized by dna-dna hybridization experiments, in which the cmr gene of plasmid pip501, of group b st ... | 1987 | 3118797 |
| region of the streptococcal plasmid pmv158 required for conjugative mobilization. | the nonconjugative streptococcal plasmid pmv158 can be mobilized by the conjugative streptococcal plasmid pip501. we determined the sequence of the 1.1-kilobase ecori fragment of pmv158 to complete the dna sequence of the plasmid. we showed that an open reading frame, mob (able to encode a polypeptide of 58,020 daltons), is required for mobilization of pmv158. an intergenic region present in the ecori fragment contains four lengthy palindromes that are found also in one or more of the staphyloco ... | 1989 | 2768188 |