curing of plasmid pe194 with novobiocin and coumermycin a1 in bacillus subtilis and staphylococcus aureus.plasmid pe194 determining macrolide-lincosamid-streptogramin b resistance could be eliminated with novobiocin and coumermycin a1 in bacillus subtilis and staphylococcus aureus. in both organisms the curing effect depended on the temperature. in the case of staphylococcus aureus it increased very much at high temperatures, while in the case of bacillus subtilis only a moderate increase could be observed. the curing activity of coumermycin was substantially weaker than that of novobiocin in both b ...19872823505
the hyaluronate lyase of staphylococcus aureus - a virulence factor?the hyaluronate lyase (hl) gene of staphylococcus aureus 8325-4 (hysa) was inactivated in vitro with the insertion of the erythromycin determinant, ermc, from plasmid pe194. the hysa : : ermc mutation was introduced into s. aureus via a temperature-sensitive shuttle vector, where it underwent homologous recombination with the wild-type (w.t.) allele. the insertion of ermc in the chromosomal hysa locus was confirmed by southern blot hybridization and the loss of hl activity was demonstrated macro ...200415184586
characterization of an erythromycin resistance (erm) plasmid in streptococcus pyogenes.three erythromyxin-resistant swedish isolates of streptococcus pyogenes, representing different t-types, were studied. two of the strains showed constitutive high-level (mic > 200 micrograms/ml) and one showed moderate (mic 6.4 micrograms/ml) resistance; the latter strain was sensitive to lincosamide and clindamycin, and resistance was not induced by erythromycin. in each of the strains, a plasmid with an estimated mw of 17.6 +/- 0.9 x 10(6) was isolated in addition to smaller cryptic plasmids. ...19957695892
illegitimate recombination in bacillus subtilis: nucleotide sequences at recombinant dna junctions.the illegitimate integration of plasmid pgg20 (the hybrid between staphylococcus aureus plasmid pe194 and escherichia coli plasmid pbr322) into the bacillus subtilis chromosome was studied. it was found that nucleotide sequences of both parental plasmids could be involved in this process. the recombinant dna junctions between plasmid pgg20 and the chromosome were cloned and their nucleotide sequences were determined. the site of recombination located on the pbr322 moiety carried a short region ( ...19873123895
replication properties of pim13, a naturally occurring plasmid found in bacillus subtilis, and of its close relative pe5, a plasmid native to staphylococcus aureus.a naturally occurring plasmid from bacillus subtilis, pim13, codes for constitutively expressed macrolide-lincosamide-streptogramin b (mls) resistance, is stably maintained at a high copy number, and exists as a series of covalent multimers. the complete sequence of pim13 has been reported (m. monod, c. denoya, and d. dubnau, j. bacteriol. 167:138-147, 1986) and two long open reading frames have been identified, one of which (ermc') is greater than 90% homologous to the ermc mls resistance deter ...19872822666
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