| fly borne vibrio parahaemolyticus in calcutta. | v. parahaemolyticus has been detected from flies in all months of the year in calcutta. the mean percentage of isolation is: housefly 14.1, blue bottle fly 18.5, external washing 10.1, viscera 22.6, flies of sweet shops 9.7 and those from fish market 22.7. flies revealed higher (4.4%) isolation of kanagawa postive v. parahaemolyticus strains than those of water and fish specimens (0.8%) studied previously. strains negative to kanagawa test but simulating enteropathogenic serotypes are recorded i ... | 1978 | 749287 |
| isolation and characterization of vibrio parahaemolyticus from cape cod soft-shell clams (mya arenaria). | vibrio parahaemolyticus was isolated from soft-shell clams (mya arenaria) taken from 10 different clamming areas on cape cod, mass., during july and august 1972. direct plating on thiosulfate-citrate-bile salts-sucrose agar was found to be superior to either direct plating on vanderzant modified salt starch agar or enrichment with trypticase soy broth containing 7% salt for isolation from clam samples. morphological and biochemical characteristics of 33 isolates from 30 samples generally conform ... | 1975 | 1096817 |
| potent antagonism of escherichia coli, bacteroides ovatus, fusobacterium varium, and enterococcus faecalis, alone or in combination, for enteropathogens in anaerobic continuous flow cultures. | interactions between representative strains of four predominant resident bacteria of the human colon, escherichia coli, enterococcus faecalis, bacteroides ovatus, and fusobacterium varium, and strains of seven enteropathogens, yersinia enterocolitica, shigella flexneri, salmonella typhimurium, vibrio parahaemolyticus, v. cholerae serogroup non o1, staphylococcus aureus, and clostridium perfringens, were examined in studies with an anaerobic continuous flow culture system and medium resembling th ... | 1986 | 2875188 |
| [the enteropathogenic role of vibrio parahaemolyticus in the coastal zone of the ivory coast]. | two clinical forms: choleriform syndrome and gastro enteritis came be individualised. the geographical origin of these cases (the coastal region abidjan) as well as some samples issued aquatic milieu enable to point out some modes of human contamination. | 1984 | 6327103 |
| vibrio vulnificus (lactose-positive vibrio) and vibrio parahaemolyticus differ in their susceptibilities to human serum. | eleven vibrio vulnificus (lactose-positive vibrio) strains were less susceptible to the bactericidal activity of normal human serum or serum treated with magnesium-ethyleneglycol-bis (beta-aminoethyl ether)-n,n'-tetraacetic acid than were six vibrio parahaemolyticus strains. | 1981 | 6788703 |
| comparative sensitivity of 13 species of pathogenic bacteria to seven chemical germicides. | the relative resistance of diverse human bacterial pathogens to commonly used germicidal agents has not been established. | 1997 | 9276546 |
| phytochemical and antimicrobial studies of begonia malabarica. | phytochemical investigation of the various extracts of the leaves of begonia malabarica lam. (begoniaceae) resulted in the isolation and identification of six known compounds, viz. friedelin, epi-friedelinol, beta-sitosterol, luteolin, quercetin and beta-sitosterol-3-beta-d-glucopyranoside. the aqueous and organic solvent extracts were also tested against ten human pathogenic bacteria and four fungal strains by the agar-well diffusion method. all the extracts were devoid of antifungal activity a ... | 2002 | 11744306 |
| epidemiology, pathogenesis, and prevention of foodborne vibrio parahaemolyticus infections. | since its discovery about 50 years ago, vibrio parahaemolyticus has been implicated as a major cause of foodborne illness around the globe. v. parahaemolyticus is a natural inhabitant of marine waters. human infections are most commonly associated with the consumption of raw, undercooked or contaminated shellfish. a few individual v. parahaemolyticus virulence factors, including the thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh), have been investigated in depth, yet a compre ... | 2004 | 15992266 |
| vibrio parahaemolyticus associated with cholera-like diarrhea among patients in north jakarta, indonesia. | a diarrhea study was conducted in north jakarta, indonesia from december 1996 through december 1997. vibrio parahaemolyticus was isolated from 333 (6.1%) of 5442 rectal swab samples collected from patients with cholera-like diarrhea. vibrio cholerae o1 was isolated from 545 (10.0%) and v. cholerae non-o1 from 183 samples (3.4%), respectively. patients positive for v. parahaemolyticus were mostly adults between 20 and 40 years of age, with males constituting 62%. a majority (65%) of these patient ... | 2001 | 11248518 |
| [characterization of haemolysis of the vibrio parahaemolyticus no.93]. | vibrio parahaemolyticus is a causative bacterium of food poisoning, and the haemolysin produced by this organism has been considered as one of the important virulence factors. in order to understand the pathogenic mechanism of this bacterium, the characteristics of haemolysin from vibrio parahaemolyticus isolated from taiwan were studied. one of the clinical strains, v. parahaemolyticus no.93, presents a weak hemolytic zone on 7% nacl-wagatsuma medium. the dna hybridization results show that v. ... | 1997 | 10592808 |
| detection of total and hemolysin-producing vibrio parahaemolyticus in shellfish using multiplex pcr amplification of tl, tdh and trh. | vibrio parahaemolyticus is an important human pathogen which can cause gastroenteritis when consumed in raw or partially-cooked seafood. a multiplex pcr amplification-based detection of total and virulent strains of v. parahaemolyticus was developed by targeting thermolabile hemolysin encoded by tl, thermostable direct hemolysin encoded by tdh, and thermostable direct hemolysin-related trh genes. following optimization using oligonucleotide primers targeting tl, tdh and trh genes, the multiplex ... | 1999 | 10379807 |
| detection of free-living and plankton-bound vibrios in coastal waters of the adriatic sea (italy) and study of their pathogenicity-associated properties. | culturable vibrios were isolated from water and plankton fractions collected during an 18-month sampling study performed along the north-central coast of the adriatic sea (italy). unculturable vibrio vulnificus and v. parahaemolyticus were detected in plankton fractions by polymerase chain reaction amplification of dna sequences for cytotoxin-haemolysin and thermolabile haemolysin respectively. the presence of v. parahaemolyticus, v. vulnificus and v. cholerae virulence genes and the expression ... | 2006 | 16817938 |
| inhibition of vibrio parahaemolyticus by a bacteriocin-like inhibitory substance (blis) produced by vibrio mediterranei 1. | the aim of this research was to identify and partially purify new bacteriocin-like substances from strains of halophilic 'non-cholera' vibrios isolated from food sources. | 2006 | 16834611 |
| phosphorylation of a 25 kda protein is induced by thermostable direct hemolysin of vibrio parahaemolyticus. | thermostable direct hemolysin (tdh) is a possible virulence factor produced by vibrio parahaemolyticus. although tdh has a variety of biological activities, including hemolytic activity, the biochemical mechanism of action remains uncertain. here we analysed biochemical events, especially phosphorylation, caused by tdh in erythrocytes, and found that tdh caused significant phosphorylations of proteins on erythrocyte membrane. phosphorylation of proteins was studies using [gamma-32p] atp and sds- ... | 1996 | 9022294 |
| correlation between cell-associated mannose-sensitive hemagglutination by vibrio parahaemolyticus and adherence to a human colonic cell line caco-2. | cell-associated hemagglutination (cha) activity with human erythrocytes was examined for 468 clinical and 71 environmental strains of vibrio parahaemolyticus. approximately 95% of the strains tested were cha positive irrespective of source or kanagawa phenomenon. 75% of clinical strains showed relatively strong mannose-sensitive hemagglutination (msha), whereas 88% of the environmental strains showed relatively weak mannose-resistant hemagglutination (mrha). adherence of v. parahaemolyticus to c ... | 1994 | 7914500 |
| virulence gene- and pandemic group-specific marker profiling of clinical vibrio parahaemolyticus isolates. | vibrio parahaemolyticus is a halophilic bacterium capable of causing food- and waterborne gastroenteritis, wound infections, and septicemia in humans. the organism has recently received increasing attention, as the emergence of a new clone, v. parahaemolyticus o3:k6, has resulted in the first documented pandemic spread of v. parahaemolyticus. we used microarray analyses to explore the presence of known virulence factors and genetic markers thought to be specific for v. parahaemolyticus o3:k6 and ... | 2007 | 17301274 |
| halophilic vibrios from human tissue infections on the pacific coast of australia. | inshore sea waters harbour a variety of halophilic bacteria which increase in the warmer seasons. ten vibrio alginolyticus, 3 v. parahaemolyticus and 3 lactose-fermenting (l +) strains of vibrio were isolated in 3 yr from wound and ear infections, salpingitis and sputum in 2 coastal towns; 14 isolations were in summer. clinically the cases resembled ordinary pyogenic infections. the api 20e kit and determination of nacl tolerance permitted identification of the strains with reasonable confidence ... | 1980 | 7432818 |
| adherence of vibrio parahaemolyticus to human epithelial cell lines. | light microscopy and a radioassay detected no significant difference in adherence of kanagawa-positive and kanagawa-negative strains of vibrio parahaemolyticus to human epithelial cell lines. | 1980 | 7377770 |
| lethal cold stress of vibrio vulnificus in oysters. | studies were conducted on the survival of vibrio vulnificus, an estuarine human pathogen, in oyster homogenates held at 4 degrees c. results indicated a rapid and dramatic decrease in viability not attributable to either cold shock or the oyster homogenate alone but to a combination of the two. such a decline was not observed with vibrio parahaemolyticus. chilled v. vulnificus cells were unable to repair themselves in brain heart infusion broth at 37 degrees c. v. vulnificus cells incubated on w ... | 1981 | 7224632 |
| adherence as a method of differentiating virulent and avirulent strains of vibrio parahaemolyticus. | usually only kanagawa-positive strains of vibrio parahaemolyticus are considered virulent; yet, a significant portion of v. parahaemolyticus food poisonings appear to be caused by kanagawa-negative strains. therefore, additional and more accurate measurements of a strain's food-poisoning potential are needed. adherence of v. parahaemolyticus to human fetal intestinal (hfi) cells in vitro seems to offer this information. all strains of v. parahaemolyticus adhered to the hfi cells, but the degree ... | 1980 | 6999994 |
| vibrio parahaemolyticus and related halophilic vibrios. | approximately 30 years have elapsed since dr. fujino's original discovery that vibrio parahaemolyticus (then termed pasteurella parahemolytica) was the cause of "summer diarrhea" in japan. since that finding, v. parahaemolyticus has been established as a cause of gastroenteritis in numbers and places approaching global proportions. it has been isolated in marine and estuarine areas almost worldwide and despite its halophilic nature, v. parahaemolyticus has been isolated from saline-free waters. ... | 1982 | 6756788 |
| wound infection caused by kanagawa-negative vibrio parahaemolyticus. | kanagawa-positive vibrio parahaemolyticus strains are considered to be human pathogens and are most commonly associated with summer diarrhea. kanagawa-negative strains are most frequently isolated from the environment and are generally considered to be nonpathogens. we report a wound infection caused by a kanagawa-negative v. parahaemolyticus strain. the infection occurred in october, an unusual time of the year for a v. parahaemolyticus infection to occur in the mid-atlantic region of the unite ... | 1984 | 6490864 |
| [vibrio parahaemolyticus--a representative of sea microflora pathogenic for man (author's transl)]. | | 1980 | 6448099 |
| plasmid patterns, serotyping and kanagawa phenomenon of vibrio parahaemolyticus isolates from man, water and fish in calcutta, india. | | 1987 | 3507428 |
| [human gastroenteritis associated with vibrio parahaemolyticus in recife, brazil]. | a study was carried out on the occurrence of vibrio parahaemolyticus in 1,100 diarrheal feces, routinely sent to a private clinical laboratory for microbiologic diagnosis, in recife. v. parahaemolyticus was isolated from 14 (1.3%) fecal samples. however, if we considered only the specimens from adult patients, the isolation rate of v. parahaemolyticus rose to 7.1%. in most cases (92.86%), v. parahaemolyticus was the only enteropathogen recognized. among the isolates, seven k antigen serovars wer ... | 1991 | 1843400 |
| demonstration of agglutinating antibodies against vibrio parahaemolyticus in the yellow tail flounder (limanda ferruginea). | sera from the salt water fish (yellow tail flounder) and the fresh water fish (rainbow trout) were tested for the presence of circulating antibodies against nine strains of vibrio parahaemolyticus and one of v. alginolyticus. thirteen out of 26 flounder sera tested gave agglutinating reactions against one specific strain of v. parahaemolyticus; strain no. 3525. seven rainbow trout sera tested failed to agglutinate any of the strains tested. all agglutinating activity was lost upon boiling the an ... | 1976 | 931748 |
| contribution of vibrio parahaemolyticus virulence factors to cytotoxicity, enterotoxicity, and lethality in mice. | vibrio parahaemolyticus, one of the human-pathogenic vibrios, causes three major types of clinical illness: gastroenteritis, wound infections, and septicemia. thermostable direct hemolysin (tdh) secreted by this bacterium has been considered a major virulence factor of gastroenteritis because it has biological activities, including cytotoxic and enterotoxic activities. previous reports revealed that v. parahaemolyticus strain rimd2210633, which contains tdh, has two sets of type iii secretion sy ... | 2010 | 20086084 |
| survival of human enteric and other sewage microorganisms under simulated deep-sea conditions. | the survival of pure cultures of escherichia coli, streptococcus faecalis, clostridium perfringens, and vibrio parahaemolyticus under simulated deep-sea conditions of low temperature (4 c), seawater, and hydrostatic pressures ranging from 1 to 1,000 atm was determined over a period exceeding 300 h. the viability of e. coli and total aerobic bacteria in seawater-diluted raw sewage subjected to these deep-sea conditions was also measured. there was a greater survival of both e. coli and s. faecali ... | 1975 | 169733 |
| interaction of thermostable direct hemolysin of vibrio parahaemolyticus with human erythrocytes. | the interaction between thermostable direct hemolysin produced by vibrio parahaemolyticus wp-1 and human erythrocytes was studied. the lysis of human erythrocytes by the hemolysin was dependent of temperature and no hemolysis occurred at low temperature (0-4 c), but the hemolysin was adsorbed on human erythrocytes even at low temperature. no hemolysis was observed when antihemolysin antiserum was mixed with the hemolysin and human erythrocytes at zero time. on the other hand, lysis of the cells ... | 1975 | 3160 |
| microarray analysis identifies apoptosis regulatory gene expression in hct116 cells infected with thermostable direct hemolysin-deletion mutant of vibrio parahaemolyticus. | the thermostable direct hemolysin (tdh) is considered as a major virulence factor of vibrio parahaemolyticus. we observed this potential in several human cancer cell lines by using the tdh-producing wild-type (rimd2210633) as well as tdh-deletion mutant of v. parahaemolyticus and found that the deletion of tdh did not affect cytotoxicity to any of the cell lines tested. dna fragmentation and annexin v staining showed that both wild-type and tdh-mutant trigger apoptosis in these cells. to underst ... | 2005 | 16061205 |
| flow cytometric evaluation of vibrio parahaemolyticus adhesion inhibition to human epithelial cells. | the present report demonstrates the usefulness of flow cytometry for a quantitative assessment of adhesion inhibition of a vibrio parahaemolyticus strain to human epithelial cells to acquire more information about the nature of its adhesins. | 2005 | 15924304 |
| occurrence, characterisation and detection of potential virulence determinants of emerging aquatic bacterial pathogens from the philippines and thailand. | strains of aeromonas spp., 'non-cholera vibrios' (ncvs) and plesiomonas shigelloides isolated from aquatic environments, fish and human diarrhoeal cases in the philippines and thailand were characterised for potential virulence markers. thus, the production of cytotoxin, cell-associated and cell-free haemolysin and their capacity to adhere to human intestinal (henle 407) cells in vitro was investigated. in addition, the occurrence of tlh and tdh haemolysin genes and urease activity among v. para ... | 2004 | 15646053 |
| generation of active fragments from human zymogens in the bradykinin-generating cascade by extracellular proteases from vibrio vulnificus and v. parahaemolyticus. | vibrio vulnificus is an opportunistic human pathogen causing septicemia, and the infection is characterized by formation of the edematous skin lesions on limbs. this pathogenic species secretes a thermolysin-like metalloprotease as a virulence determinant. the metalloprotease was confirmed to activate human factor xii-plasma kallikrein-kinin cascade that results in liberation of bradykinin, a chemical mediator enhancing the vascular permeability, from high-molecular weight kininogen. namely, the ... | 2004 | 15530971 |
| cholera and other types of vibriosis: a story of human pandemics and oysters on the half shell. | vibrios are ubiquitous in the aquatic environment and are commonly present in or on shellfish and other seafood. a small subset of strains/species are able to cause human disease, including the cholera toxin-producing strains of vibrio cholerae that are responsible for epidemic/pandemic cholera; thermostable direct hemolysin-producing strains of vibrio parahaemolyticus; and vibrio vulnificus, which can cause fulminant sepsis. cholera outbreaks can be initiated by transmission of "epidemic" v. ch ... | 2003 | 12856219 |
| characterization by pcr of vibrio parahaemolyticus isolates collected during the 1997-1998 chilean outbreak. | between november 1997 and april 1998, several human gastroenteritis cases were reported in antofagasta, a city in the north of chile. this outbreak was associated with the consumption of shellfish, and the etiologic agent responsible was identified as vibrio parahaemolyticus. this was the first report of this bacterium causing an epidemic in chile. v. parahaemolyticus was the only pathogenic bacterium isolated from patient stools and from shellfish samples. these isolates were analyzed by polyme ... | 2002 | 12462995 |
| molecular analysis of vibrio parahaemolyticus isolated from human patients and shellfish during us pacific north-west outbreaks. | aims: the objective of this study was to investigate the occurrence and distribution of haemolysin genes, plasmid profile, serogroup analysis and cellular urease activity for vibrio parahaemolyticus isolates from infected human patients and oysters from the pacific north-western united states between 1988 and 1997. methods and results: all of the clinical and environmental isolates tested in this study exhibited the presence of the thermolabile haemolysin gene, tl, confirming that all of the iso ... | 2002 | 11874534 |
| monitoring of multiple drug-resistant pathogens in a selected stretch of bay of bengal, india. | the present work aims at identification of multiple drug-resistant pathogenic bacteria in a selected stretch, namely, puri on the bay of bengal, india. six stations at the coast of puri were selected and samples of water and sediment were collected during the winter of 2008 and 2009 for this study. thirty-eight pathogenic bacteria were isolated and identified from both the water and the sediment of 6 fixed stations (pu-1a, pu-1b, pu-2, pu-3, pu-4, and pu-5). the identified pathogens were escheri ... | 2011 | 21424669 |
| [vibrio parahaemolyticus infections and algal intoxications as emergent public health problems in chile]. | there is interest in the paradigm that relates environmental sea changes to the emergence of diseases that affect both aquatic organisms in the sea and human beings. the emergence of vibrio parahaemolyticus as an important cause of epidemic summer diarrhea in 2004 and 2005, confined mainly to the tenth region in chile, could be a manifestation of this trend. this and other areas of the country have also experienced several outbreaks of paralytic shellfish poisoning (psp), diarrheal shellfish poi ... | 2005 | 16311702 |
| comparative hemolytic activity of vibrio parahaemolyticus and related vibrios. | the hemolytic activities of 91 strains of vibrio parahaemolyticus isolated from human diarrheal stools, sea fish, and sea water; 21 suspected v. parahaemolyticus cultures isolated from wound infections; 14 nonpathogenic marine vibrios; and 21 v. parahaemolyticus isolated from moribund blue crabs callinectes sapidus were compared. potentially pathogenic v. parahaemolyticus strains could be differentiated from the related nonpathogenic marine vibrios, because the former hemolyzed hamster, sheep, a ... | 1970 | 16557747 |
| salmonella spp., vibrio spp., clostridium perfringens, and plesiomonas shigelloides in marine and freshwater invertebrates from coastal california ecosystems. | the coastal ecosystems of california are highly utilized by humans and animals, but the ecology of fecal bacteria at the land-sea interface is not well understood. this study evaluated the distribution of potentially pathogenic bacteria in invertebrates from linked marine, estuarine, and freshwater ecosystems in central california. a variety of filter-feeding clams, mussels, worms, and crab tissues were selectively cultured for salmonella spp., campylobacter spp., escherichia coli-o157, clostrid ... | 2006 | 16897302 |
| identification and characterization of vopt, a novel adp-ribosyltransferase effector protein secreted via the vibrio parahaemolyticus type iii secretion system 2. | vibrio parahaemolyticus strain rimd2210633 has two sets of genes encoding two separate type iii secretion systems (t3sss), called t3ss1 and t3ss2. t3ss2 has a role in enterotoxicity and is present only in kanagawa phenomenon-positive strains, which are pathogenic to humans. accordingly, t3ss2 is considered to be closely related to v. parahaemolyticus human pathogenicity. despite this, the biological actions of t3ss2 and the identity of the effector protein(s) secreted by this system have not bee ... | 2007 | 17645751 |
| complement-mediated killing of vibrio species by the humoral fluids of amphioxus branchiostoma belcheri: implications for a dual role of o-antigens in the resistance to bactericidal activity. | the functional properties of complement in invertebrate deuterostomes are rather ill-defined. here we showed that the humoral fluids from amphioxus branchiostoma belcheri were capable of causing lysis of some vibrio species including vibrio alginolyticus hw284, vibrio parahaemolyticus hw458 and vibrio harvey sf-1, the first such data in the invertebrate deuterostomes. the fluid bacteriolytic activity was abolished by pre-incubation with heat-inactivated rabbit anti-human c3 serum, heating at 45 ... | 2008 | 18178103 |
| a model of the effect of temperature on the growth of pathogenic and nonpathogenic vibrio parahaemolyticus isolated from oysters in korea. | vibrio parahaemolyticus is recognized as the leading cause of human gastroenteritis associated with the consumption of seafood. the objective of this study was to model the growth kinetics of pathogenic and nonpathogenic v. parahaemolyticus in broth and oyster slurry. primary growth models of v. parahaemolyticus in broth and oyster slurry fit well to a modified gomperz equation (broth r(2)=0.99; oyster slurry r(2)=0.96). the lag time (lt), specific growth rate (sgr), and maximum population densi ... | 2008 | 18541160 |
| contamination of community water sources by potentially pathogenic vibrios following sea water inundation. | potentially pathogenic members of the vibrionaceae family including vibrio cholerae and vibrio parahemolyticus were isolated from domestic sources of drinking water in coastal villages following sea water inundation during the tsunami in southern india. phenotypic and genotypic studies were done to confirm the identity and detection of toxins. vibrio-gyr (gyrase b gene) was detected in all sixteen vibrio isolates. toxin regulating genes i.e.: ctx gene, tdh gene, and trh gene, however were not de ... | 2007 | 18697592 |
| identification and characterization of a novel type iii secretion system in trh-positive vibrio parahaemolyticus strain th3996 reveal genetic lineage and diversity of pathogenic machinery beyond the species level. | vibrio parahaemolyticus is a bacterial pathogen causative of food-borne gastroenteritis. whole-genome sequencing of v. parahaemolyticus strain rimd2210633, which exhibits kanagawa phenomenon (kp), revealed the presence of two sets of the genes for the type iii secretion system (t3ss) on chromosomes 1 and 2, t3ss1 and t3ss2, respectively. although t3ss2 of the rimd2210633 strain is thought to be involved in human pathogenicity, i.e., enterotoxicity, the genes for t3ss2 have not been found in trh- ... | 2009 | 19075025 |
| identification and characterization of a type iii secretion-associated chaperone in the type iii secretion system 1 of vibrio parahaemolyticus. | vibrio parahaemolyticus causes human gastroenteritis. genomic sequencing of this organism has revealed that it has two sets of type iii secretion systems, t3ss1 and t3ss2, both of which are important for its pathogenicity. however, the mechanism of protein secretion via t3sss is unknown. a characteristic of many effectors is that they require specific chaperones for efficient delivery via t3sss; however, no chaperone has been experimentally identified in the t3sss of v. parahaemolyticus. in this ... | 2009 | 19459967 |
| benthic ecology of vibrio spp. and pathogenic vibrio species in a coastal mediterranean environment (la spezia gulf, italy). | we carried out a 16-month in situ study to investigate the ecology of vibrio spp. and pathogenic vibrio species in coastal sediments of the mediterranean sea, employing multiple-regression analysis to reveal the major environmental factors controlling their occurrence in the benthic environment. in addition, association between vibrios and sediment-inhabiting meiofauna, which is a major component of benthic ecosystems, was investigated. culturable and total vibrio spp. estimates by most-probable ... | 2009 | 19543938 |
| a novel fas ligand in mollusk abalone: molecular characterization, immune responses and biological activity of the recombinant protein. | fas ligand is a member of the tnf superfamily that plays an important role by inducing apoptosis and homeostasis of immune responses. the gene encoding fas ligand was isolated from a disk abalone (haliotis discus discus) cdna library, denoted as the abfas ligand. it contains an 1832bp transcript with a 945bp open reading frame, encoding 315 amino acids. the abfas ligand showed characteristic transmembrane and tnf family signature domains. the deduced amino acid comparison showed that the abfas l ... | 2009 | 19576285 |
| vibrio vopq induces pi3-kinase-independent autophagy and antagonizes phagocytosis. | vibrio parahaemolyticus is a gram-negative bacterium responsible for gastroenteritis acquired from the consumption of contaminated shellfish. this bacterium harbours two type iii secretion systems, one on each chromosome. the type iii secretion system on chromosome i induces cell death by a temporally controlled sequence of events that is caspase-independent and first involves induction of autophagy, followed by cellular rounding, and finally cellular lysis. vopq is a type iii secreted effector ... | 2009 | 19627496 |
| disk abalone (haliotis discus discus) expresses a novel antistasin-like serine protease inhibitor: molecular cloning and immune response against bacterial infection. | a novel antistasin-like cdna homologue named as ab-antistasin was isolated from the disk abalone haliotis discus discus normalized cdna library. the ab-antistasin (1398-bp) consisted of an 1185-bp open reading frame encoding 395 amino acid (aa) residues. the predicted molecular mass and isoelectric point of ab-antistasin was 44 kda and 8.5, respectively, and showed highest identity (23.1%) to hydra magnipapillata antistasin. the most striking feature of ab-antistasin is the 12-fold internal repe ... | 2010 | 20060477 |
| immunoproteomic identification of polyvalent vaccine candidates from vibrio parahaemolyticus outer membrane proteins. | bacterium is still a major cause of many infectious diseases and a global threat to human health, aquaculture, and animal feeding. prevention by vaccination is the most efficient and economical way of fighting bacterial diseases, but one of the persistent challenges to prevent bacterial infections and disease transmissions is the existence of multiple bacterial species, families, and genera and the lack of efficient polyvalent vaccines against them. the information on candidate immunogens for po ... | 2010 | 20148598 |
| brichos domain-containing leukocyte cell-derived chemotaxin 1-like cdna from disk abalone haliotis discus discus. | a brichos domain-containing leukocyte cell-derived chemotaxin 1-like cdna was cloned from the disk abalone (haliotis discus discus) and designated as ablect-1. a full-length (705 bp) of ablect-1 cdna was composed of a 576 bp open reading frame that translates into a putative peptide of 192 amino acids. deduced amino acid sequence of ablect-1 had 15.5- and 27.8% identity and similarity to human lect-1, respectively. quantitative real-time pcr analysis results showed that the mrna of ablect-1 was ... | 2010 | 20688173 |
| first molluscan transcription factor activator protein-1 (ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury. | the regulation of transcriptional activation is an essential and critical point in gene expression. in this study, we describe a novel transcription factor activator protein-1 (ap-1) gene from disk abalone haliotis discus discus (abap-1) for the first time in mollusk. it was identified by homology screening of an abalone normalized cdna library. the cloned abap-1 consists of a 945 bp coding region that encodes a putative protein containing 315 amino acids. the abap-1 gene is composed of a charac ... | 2010 | 20732431 |
| comparison of the pathogenic potentials of environmental and clinical vibrio parahaemolyticus strains indicates a role for temperature regulation in virulence. | although the presence of pathogenic vibrio spp. in estuarine environments of northern new england has been known for some time (c. h. bartley and l. w. slanetz, appl. microbiol. 21: 965-966, 1971, and k. r. o'neil, s. h. jones, and d. j. grimes, fems microbiol. lett. 60:163-167, 1990), their virulence and the relative threat they may pose to human health has yet to be evaluated. in this study, the virulence potential of 33 vibrio parahaemolyticus isolates collected from the great bay estuary of ... | 2010 | 20889774 |
| lactic acid as a potential decontaminant of selected foodborne pathogenic bacteria in shrimp (penaeus merguiensis de man). | fresh raw shrimps were dipped for 10, 20, and 30 min at room temperature (25°c ± 1°c) in lactic acid (la; 1.5%, 3.0%, v/v) to evaluate their antipathogenic effects against vibrio cholerae, vibrio parahaemolyticus, salmonella entreitidis, and escherichia coli o157:h7 inoculated at a level of 10(5) cfu/g. significant reductions in the population of all these pathogenic bacteria were recorded after dipping treatments, which were correlated to the corresponding la concentrations and treatment time. ... | 2010 | 21034165 |
| conventional and molecular methods to detect bacterial pathogens in mussels. | to detect aeromonas spp., salmonella spp., vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus in mussels and water samples from a farming area, conventional and molecular methods were applied to enrichment cultures. | 2010 | 21070267 |
| multiple antibacterial histone h2b proteins are expressed in tissues of american oyster. | we have previously identified a histone h2b isomer (cvh2b-1) from tissue extracts of the bivalve mollusk, the american oyster (crassostrea virginica). in this paper, we isolate an additional three antibacterial proteins from acidified gill extract by preparative acid-urea-polyacrylamide gel electrophoresis and reversed-phase high performance liquid chromatography. extraction of these proteins from tissue was best accomplished by briefly boiling the tissues in a weak acetic acid solution. additio ... | 2010 | 21145411 |
| enumeration of vibrio parahaemolyticus in the viable but nonculturable state using direct plate counts and recognition of individual gene fluorescence in situ hybridization. | vibrio parahaemolyticus is a gram-negative, halophilic bacterium indigenous to marine and estuarine environments and it is capable of causing food and water-borne illness in humans. it can also cause disease in marine animals, including cultured species. currently, culture-based techniques are used for quantification of v. parahaemolyticus in environmental samples; however, these can be misleading as they fail to detect v. parahaemolyticus in a viable but nonculturable (vbnc) state which leads t ... | 2011 | 21329738 |
| evaluation of a new chromogenic medium, chromid™ vibrio, for the isolation and presumptive identification of vibrio cholerae and vibrio parahaemolyticus from human clinical specimens. | the aim of this study was to evaluate the performance of the chromid™ vibrio medium for the detection of vibrio cholerae and v. parahaemolyticus in stool and swab specimens in comparison with thiosulfate citrate bile salts sucrose (tcbs) medium. a total of 96 samples including 30 fresh stool, 32 stool, and 34 swab specimens originating from routine laboratories were tested. all samples were seeded on both media, the tcbs medium and the chromid vibrio, directly and after an enrichment step on alk ... | 2011 | 21484255 |
| purification, crystallization and preliminary x-ray crystallographic analysis of the putative vibrio parahaemolyticus resuscitation-promoting factor yeaz. | vibrio parahaemolyticus is a human pathogen associated with gastroenteritis caused by the ingestion of contaminated raw seafood. v. parahaemolyticus is able to survive exposure to low temperatures typical of those used for the refrigeration of foods by entering a viable but nonculturable (vbnc) state. the vbnc cells can regain culturability and renewed ability to cause infection upon temperature upshift. the resuscitation-promoting factor protein (rpf, yeaz) plays a key role in reactivation of g ... | 2011 | 21543872 |
| vibrio parahemolyticus septicaemia in a liver transplant patient: a case report. | abstract: introduction: vibrio parahemolyticus is the leading cause of vibrio-associated gastroenteritis in the united states of america, usually related to poor food handling; only rarely has it been reported to cause serious infections including sepsis and soft tissue infections. in contrast, vibrio vulnificus is a well-known cause of septicaemia, especially in patients with cirrhosis. we present a patient with v. parahemolyticus sepsis who had an orthotic liver transplant in 2007 and was on i ... | 2011 | 21548914 |
| vibrio parahaemolyticus cell biology and pathogenicity determinants. | vibrio parahaemolyticus is a significant cause of gastroenteritis worldwide. characterization of this pathogen has revealed a unique repertoire of virulence factors that allow for colonization of the human host and disease. the following describes the known pathogenicity determinants while establishing the need for continued research. | 2011 | 21782964 |
| gut cavp is an innate immune protein against bacterial challenge in amphioxus branchiostoma belcheri. | the importance of calcium-binding proteins in immune response of vertebrates is determined, but whether they have the role in invertebrates is largely unknown. in the present study, phylogenetic analysis indicated that calcium vector protein (cavp), a protein unique to amphioxus, shared 68% similarity in amino acid sequence with human and mouse calmodulin (cam). cavp cdna was cloned into a bacterial vector pet-32a, and its his-tagged fusion protein was produced in eschherichia coli cells (bl21). ... | 2011 | 21624472 |
| The structural pathway for water permeation through sodium-glucose cotransporters. | Although water permeation across cell membranes occurs through several types of membrane proteins, the only permeation mechanism resolved at atomic scale is that through aquaporins. Crystallization of the Vibrio parahaemolyticus sodium-galactose transporter (vSGLT) allows investigation of putative water permeation pathways through both vSGLT and the homologous human Na-glucose cotransporter (hSGLT1) using computational methods. Grand canonical Monte Carlo and molecular dynamics simulations were ... | 2011 | 22004742 |
| Lactobacillus plantarum AS1 binds to cultured human intestinal cell line HT-29 and inhibits cell attachment by enterovirulent bacterium Vibrio parahaemolyticus. | Lactobacillus plantarum AS1 was incubated with HT-29 adenocarcinoma cell line to assess its adhesion potency and examined for its inhibitory effect on the cell attachment by an enterovirulent bacterium Vibrio parahaemolyticus. | 2011 | 21848906 |
| molecular characterization of ran gene up-regulated in large yellow croaker (pseudosciaena crocea) immunity. | rangtpase, one family of small g protein superfamily, has been widely demonstrated to be involved in transport system between cytoplasm and nucleus. however the knowledge about the function of rangtpase in immunity remains limited. in this report, ran gene (named lycran) cdna was cloned from the large yellow croaker, pseudosciaena crocea, a marine fish. the full-length cdna of lycran was of 1033 bp, including a 5'-terminal untranslated region (utr) of 43 bp, 3'-terminal utr of 338 bp and an open ... | 2010 | 20447461 |
| high amount of dinophysistoxin-3 in mytilus chilensis collected in seno de reloncaví, chile, during massive human intoxication associated with outbreak of vibrio parahaemolyticus. | this study describes the detection of high amount of 7-o-acyl-derivative dinophysistoxin-1 (dinophysistoxin-3) in filter bivalves collected on february 2005 in the seno de reloncaví, puerto montt city, southern chile, in the same period of time where an intoxication episode was associated with the presence of vibrio parahaemolyticus in shellfish. the diarrhetic shellfish poisoning (dsp) mouse bioassay of mussel extract samples, performed as described for regulatory testing, were negative to dsp ... | 2006 | 17077585 |
| hydrophobicity, cell adherence, cytotoxicity, and enterotoxigenicity of starved vibrio parahaemolyticus. | vibrio parahaemolyticus is a ubiquitous gram-negative enteropathogenic bacterium that may encounter starvation or other environmental stresses during food processing or human infection. pathogenic v. parahaemolyticus st550 cultures starved in modified morita mineral salt solution with 3 or 0.5% nacl exhibited similar resistance against challenges of environmental stresses. changes in virulence of the starved v. parahaemolyticus was determined using hep-2 cell culture and suckling mouse assay. th ... | 2005 | 15690818 |
| genome sequence of a clinical o4:k12 serotype vibrio parahaemolyticus strain 10329. | vibrio parahaemolyticus is the leading cause of foodborne illnesses worldwide. here we report a draft genome of v. parahaemolyticus strain vp10329 of o4:k12 serotype. it belongs to the main u. s. west coast clonal complex of v. parahaemolyticus (st36) causing oyster-associated human illness. it contains the virulence determinants tdh and trh but appears to infect at much lower doses than v. parahaemolyticus strains with these same determinants from other areas such as the u.s. gulf and atlantic ... | 2011 | 21551294 |
| quantitative risk assessment of vibrio parahaemolyticus in finfish: a model of raw horse mackerel consumption in japan. | the aim of this study was to evaluate the effects of implemented control measures to reduce illness induced by vibrio parahaemolyticus (v. parahaemolyticus) in horse mackerel (trachurus japonicus), seafood that is commonly consumed raw in japan. on the basis of currently available experimental and survey data, we constructed a quantitative risk model of v. parahaemolyticus in horse mackerel from harvest to consumption. in particular, the following factors were evaluated: bacterial growth at all ... | 2010 | 20626688 |