Publications

TitleAbstractYear
Filter
PMID
Filter
diastereoisomeric glucuronides of oxazepam. isolation and stereoselective enzymic hydrolysis.oxazepam glucuronide isolated from swine urine by previously published methods was separated into its diastereoisomers by ion-exchange chromatography on a preparative scale. quantitative high-performance liquid chromatography was used to monitor the separation. the two isomers were obtained in analytically pure form and then characterized by elemental analysis, oxazepam content, mass spectrometry, ultraviolet spectroscopy, optical rotation and optical rotatory dispersion-circular dichroism. the ...197935325
an attempt to identify the intestinal receptor for the k88 adhesin by means of a haemagglutination inhibition test using glycoproteins and fractions from sow colostrum.the k88 antigen of escherichia coli specifically adheres to the piglet intestinal cell; a solution of this antigen agglutinates guinea-pig red cells at 4 degrees c. the latter reaction was used as a model of the former, using inhibition of haemagglutination as an index of specific combination with the k88 adhesin. inhibition was found with mucous glycoproteins and chemical modification of their heterosaccharide residues by mild acid hydrolysis, periodate oxidation or the smith degradation proced ...197546271
mucosal adherence of human enteropathogenic escherichia coli.an in-vitro assay system has been developed to measure bacterial adhesion to the mucosa of human fetal small intestine. two strains of escherichia coli that are proven human enteropathogens (e.p.e.c.) have been shown to adhere in large numbers, compared with control organisms. the attachment mechanism is species specific and is not caused by common fimbriae. mucosal adhesion may be as important as enterotoxin production or invasiveness in determining the virulence of some strains of human e.p.e. ...197553431
immunological aspects of cell membrane adhesion demonstrated by porcine enteropathogenic escherichia coli. 197875179
escherichia coli strains that cause diarrhoea but do not produce heat-labile or heat-stable enterotoxins and are non-invasive.three enteropathogenic escherichia coli (e.p.e.c.) strains (o127:k63:h6, o128:k67:h2, and o142:k86:h6) isolated from outbreaks of infantile diarrhoea and one strain from the "normal" colonic flora (e. coli hs) of a healthy adult were fed in doses of 10(6), 10(8), and 10(10) organisms in nahco3 to adult volunteers. the strains, which had been stored for 7--9 years, gave negative results in sensitive tests for heat-labile (l.t.) enterotoxin (y-1 adrenal-cell test), heat-stable (s.t.) enterotoxin ( ...197877415
relationships among raffinose plasmids determined by the immunochemical cross-reaction of their alpha-galactosidases.plasmid-encoded alpha-galactosidase served as a marker enzyme for the recognition and comparison of raffinose (raf) plasmids present in strains of escherichia coli. immunochemical relationships were established among raf plasmids of 39 independent isolates from man and domestic animals (from three continents) by using antiserum against alpha-galactosidase. immunodiffusion revealed three serological subclasses of alpha-galactosidase, which are correlated with the biological and geographical origi ...197994343
incidence and relationship of group d streptococci with other indicator organisms in meats.raw and processed meats were analyzed for presumptive group d streptococci using kf streptococcus agar. counts were compared with coliform, presumptive escherichia coli, and enterobacteriaceae counts but no meaningful relationships were observed. results indicated that group d streptococci and e. coli type i were principally contaminants from the packing plant, rather than at retail level. the predominating group d streptococcus in both beef and pork cuts was streptococcus faecalis, while in pro ...1978106944
[demonstration of enterotoxin-forming pseudomonas aeruginosa strains in the intestinal ligature test in piglets]. 1979119390
[preliminary results of an atiepizootic field test using edta-na extract vaccine from escherichia coli strains pathogenic to swine in swine production industrial units].an anti-epizootic field test was applied to industrialised pig farms in the region of wrocław, poland, to test the effectiveness of a pentavalent edta (calcium disodium edetate)--sodium vaccine extracted from escherichia coli strains with pathogenicity to swine. the vaccine had been received from a centre in berlin-buch, gdr. the vaccine failed to provide any protection, when orally applied to nursed piglets. however, both morbidity and mortality were reduced and, thus, an anti-epizootic effect ...1979121226
[observations on antibacterial activity of thiamphenicol glycinate acetylcysteinate (author's transl)].in vitro thiamphenicol antibacterial activity in the presence of n-acetylcysteine as sodium salt proved to be enhanced when compared with that of the antibacterial alone against both gram-positive and gram-negative bacterial strains. the in vitro results were confirmed in vivo in the guinea-pig, where the sera from animals previously treated by i.m. route with thiamphenicol glycinate acetylcysteinate, showed a higher antibacterial activity than sera from animals treated with thiamphenicol glycin ...1979122158
comparison of the effect of various antisera and cobra venom factor on inflammatory reactions in guinea-pig skin. ii. the arthus reaction and the local shwartzman reaction.the ability of antisera to guinea-pig c3 to inhibit the arthus and local shwartzman reactions was studied. they were found to reduce the non-haemorrhagic component of the active and reversed passive arthus reactions and to delay the appearance of the haemorrhage in the active arthus reaction. cobra venom factor, however, had no effect on the non-haemorrhagic components of these reactions and only delayed the appearance of the haemorrhage of the active arthus reaction. there appeared to be a corr ...1975125318
electron paramagnetic resonance spectra of myeloperoxidase in polymorphonuclear leukocytes. 1978202508
mechanisms in the pathogenesis of diarrhea: a review. 1978203563
study of the in vitro pharmacological effects of gram-negative endotoxins in parallel with toxicity testing in normal adult guinea-pigs. 1978206397
the effect of cholera toxin and heat labile and heat stable escherichia coli enterotoxin on cyclic amp concentrations in small intestinal mucosa of pig and rabbit.the effect of cholera toxin, heat labile and heat stable escherichia coli enterotoxin on mucosal cyclic amp concentrations was determined on the proximal jejunum of weanling pigs and young rabbits. ligated loops were injected with solutions containing no enterotoxin for control and either cholera toxin, heat labile or heat stable e. coli enterotoxin. the loops were drained after either two, four or six hours incubation at which time accumulated fluid was recorded and mucosal samples removed for ...1978210913
panel report on the colloquium on selected diarrheal diseases of the young. 1978212391
naturally occurring and experimentally induced rotaviral infections of domestic and laboratory animals. 1978212392
elisa: enzyme-linked immunosorbent assay.similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. the end point is a color change that can be assessed by colorimetry or with the naked eye. various techniques of elisa are described, along with examples of current and potential clinical applications.1978215505
contamination of the deep tissues of carcasses by bacteria present on the slaughter instruments or in the gut. 1979222722
the aetiology of diarrhoea in pigs weaned at two days of age.when pigs are weaned at two days of age large numbers of excherichia coli appear in the anterior gut and the incidence of diarrhoea rises. the two phenomena do not appear to be directly related because the strains of e coli isolated are not serotypes previously found to be associated with neonatal pig scouring. representative strains of the non-enteropathogenic serotypes did not produce enterotoxin and did not adhere to small intestine brush borders. moreover when antibiotics were fed to elimina ...1979228367
occurrence of k99 antigen on escherichia coli isolated from pigs and colonization of pig ileum by k99+ enterotoxigenic e. coli from calves and pigs.several strains of enterotoxigenic escherichia coli (etec) isolated from pigs were found to have an antigen (k99) previously reported only on strains of calf and lamb origin and which facilitates intestinal colonization in the latter two species. several human etec were also tested for k99; however, none were positive. each of four k99-positive etec strains of calf origin and one of pig origin produced k99 in pig ileum in vivo, adhered to villous epithelium in pig ileum, colonized pig ileum, and ...1977321356
[coagulation analytical studies in coli enterotoxemia in swine].store pigs with spontaneous outbreaks and experimental endotoxin shock were kept under observation in the context of coagulation analysis. heparin was applied to some of the animals to disrupt the plasmatic coagulation system. the thrombocyte count in animals with endotoxin infusion declined by some 50 to 65 percent of the original level. no statistically secured difference was found to exist between heparinised animals, on the one hand, and non-heparinised, on the other. the aggregation and adh ...1977322631
colonization of porcine small intestine by escherichia coli: colonization and adhesion factors of pig enteropathogens that lack k88.the colonizing and adhesive attributes of enterotoxigenic acapsular and/or nonpiliated mutants from k88-negative enteropathogenic escherichia coli strains were compared with their capsulated and piliated parents (parents were piliated when grown in vitro and in vivo). acapsular, nonpiliated mutants from three different colonizing strains of enteropathogenic e. coli lost their ability to colonize the ileum of newborn pigs. acapsular, piliated and capsular, nonpiliated mutants were derived from on ...1977323375
interactions of radio-detoxified escherichia coli endotoxin preparations with the complement system.escherichia coli o89 lipopolysaccharide (lps) was treated with different doses of gamma irradiation (5, 10, 15, and 20 mrad). various biological activities such as lethal effect, decrease in arterial blood pressure in dogs, and interaction with the complement system were determined for the parent and irradiated preparations. irradiation of lps significantly and in a dose-dependent manner decreased its lethal and blood pressure-depressing effects along with its ability to activate the complement ...1977326671
r-factor-mediated antibiotic resistance in e. coli strains isolated from piglets in sweden.e. coli strains, isolated from piglets over the periods 1964-1968 and 1974-1975, were investigated and compared with respect to antibiotic resistance. the frequency of monoresistant strains decreased from 50% in 1964-68 to 27% in 1974-75, while that of strains showing double or multiple resistance increased from 13% to 22%. the proportions of the different resistance determinants were as follows (figures for 1964-68 within parentheses): tetracycline 23% (49%), sulphaisodimidine 38% (24%), strept ...1977329617
a microbiological study of gastro-enteritis in black infants.a survey was carried out to determine the aetiological agents responsible for acute gastro-enteritis in black infants. bacteriological examination of 191 patients and 178 age-matched controls showed that salmonellae and shigellae together accounted for only 15% of cases and were harboured by almost 7% of controls. seropositive 'enteropathogenic' escherichia coli was an important cause of diarrhoea, especially in the summer months. enterotoxin-producing e. coli was not a significant cause of gast ...1977331509
[experimental studies on the pathogenesis of coli-enterotoxemia in swine. 4. effect of lipopolysaccharide endotoxin on weaned piglets following parenteral administration].ten clinically intact weaned piglets were experimentally intoxicated by intravenous injection of lipoproteide-free lipopolysaccharide endotoxin according to westphal of e. coli o 127:b8. severe endotoxin shock with all clinical manifestations of experimental coli-enterotoxaemia was induced in all animals and included circulatory disorder with tachycardia, intermittent pallor and/or cyanosis, symptoms of severe systemic intoxication, neurological symptoms, such as lack of coordination, hindleg st ...1977332109
[enterotoxin-producing e.-coli bacteria in enteritis and edema disease of swine. quick diagnosis of heat-labile and heat resistant toxin]. 1977332152
breeding record analysis in pig herds and its veterinary applications--2: experiences with a large commercial unit.a computer program was used to analyse data from a large commercial pig unit over two years to assess reproductive efficiency and the efficiency of weaner production. monthly inspections of the farm and quarterly post mortem examinations of all dead pigs provided further information. the effects of controlled changes in management on production were assessed. overall the number of piglets reared per sow per year was increased from 15-1 to 17-2 and the eight-week weights improved from a mean of 1 ...1977335631
uptake of maternal antibody by the neonatal pig following intramuscular and intramammary vaccination of the preparturient sow.administration of heat inactivated escherichia coli antigens by intramuscular and intramammary routes induced elevated antibody levels in sow serum and colostrum, predominantly associated with igg. colostral igg accounted for approximately 80 per cent of the total antibody activity, and there was a similar distribution in the sera of one-day-old piglets. the additional antibody activity was carried almost entirely by igm following intramuscular injections and was evenly distributed between igm a ...1977337428
effects of various nutrient levels and enviromental temperatures on the incidence of colibacillary diarrhea in pigs: intestinal fistulation and titration studies. 1977340437
the sources of "oh" serotypes of escherichia coli.the sources from which different "oh" serotypes of escherichia coli have been isolated are listed in a series of tables. it is considered that these tables should provide a basis for obtaining a greater understanding of the ecology of e. coli.1978340583
alternatives to aspirin, derived from biological sources. 1977343554
detecting enteropathogenic escherichia coli strains of porcine origin. 1. correlations between o and k antigens and the enterotoxin production in strains isolated from the newborn piglet.a study of enterotoxin production by means of biological tests (y1 adrenal cells and suckling mice) using 67 escherichia coli strains responsible for diarrhea in newborn piglets, confirmed the advantages of these methods and demonstrated the correlation between antigenic specificity and enterotoxin production. in particular the presence of capsular antigen k 88 is related to the thermolabile (lt) fraction of the enterotoxin in 73.9 % of the enteropathogenic strains studied.1977343700
hydrogen sulphide-positive strains of escherichia coli from swine.thirty-two h2s-positive strains of escherichia coli, isolated from the caecal contents or mesenteric lymph nodes or both of 60 apparently healthy pigs, were characterized. eighteen different serotypes and 18 different fermentative types were found. during conjugation with e. coli k12, four strains transferred their hys plasmids linked to raf, and another transferred hys associated with the tc determinant. all hys-raf plasmids belong to incompatibility group fi, whilst tc-hys is an fi- plasmid.1978351185
in vitro adhesion of escherichia coli to porcine small intestinal epithelial cells: pili as adhesive factors.escherichia coli strains with pili (k99 or 987p) known to facilitate intestinal colonization adhered in vitro to porcine intestinal epithelial cells. these strains adhered equally to both ileal and jejunal epithelial cells. a laboratory e. coli strain that has type 1 pili also adhered to porcine intestinal epithelial cells. when nonpiliated cells derived from 987p+, k99+, or type 1 pilus+ strains were used for in vitro adhesion assays, they failed to adhere. the attachment of piliated bacteria t ...1978357285
biological evaluation of a methanol-soluble, heat-stable escherichia coli enterotoxin in infant mice, pigs, rabbits, and calves.escherichia coli p16 was shown to produce two heat-stable toxins (st) with differing biological activity. the toxins were separated by methanol extraction, and the first, sta, was methanol soluble, partially heat stable, active in neonatal piglets (1 to 3 days old) and infant mice, but inactive in weaned pigs (7 to 9 weeks old); the second, stb, was methanol insoluble, active in weaned pigs and rabbit ligated loops, but inactive in infant mice. it is therefore suggested that use of suckling mice ...1978357288
[various aspects of competitive relationship between shigella and escherichia coli].one of the aspects of antagonistic relations could be the competence of microorganisms for specific sites of attachement to the epithelium common for shigellae and some e. coli serological types; this was demonstrated on a model of shigella-induced keratoconjunctivitis and in experiments with the hep-2 cells infected with h3-glucose-labeled sh. flexneri 5a, with combined administration of the latter with e. coli 08 and 028. the data obtained emphasized the importance for the development of the i ...1978358685
[natural antibodies (isohemagglutinins, heterophilic, antierythrocytic and antibacterial) in children with congenital immunologic deficiency syndromes].examination of 30 patients with immunodeficient diseases showed that the system of natural antibodies was considerably changed depending on the form and the extent of deficiency of the immune system: in agammaglobuinemia the antibodies under study were almost completely absent, and in immunological insufficiency with ataxia-teleangiectasis the production of antibacterial antibodies proved to be sharply decreased. the data obtained can be used both for the diagnosis of immunodeficiencies and for ...1978358690
proteinase, lipase and amylase activities in the small intestine of pigs suffering from colienterotoxaemia. 1978358811
superiority of human complement for assaying bacterial lipopolysaccharides by their anticomplementary activity.in assaying bacterial lipopolysaccharides (lps) for anticomplementary activity, human complement (c) allowed detection of approximately 200 times smaller amounts of lps than guinea-pigs c. pig c was slightly inferior to human.1978363447
the influence of plasmid-determined and other characteristics of enteropathogenic escherichia coli on their ability to proliferate in the alimentary tracts of piglets, calves and lambs. 1978364065
[production of live attenuated vaccine against colibacillosis in pigs].two strains of beta-hemolytic escherichia coli, p-0141 and i-0149 were used to obtain mutant e. coli organisms with unchanged cultural, morphological, biochemical, and serological properties. the mutants proved apathogenic for albino mice at intravenous injection of a 6-hour broth culture at the rate of 0.3 cm3 or an endotoxin in the same dose. neither were they enteropathogenic for pigs when inoculated into intestinal segments. they could be used as vaccinal strains.1978369110
studies on escherichia coli in pigs v. determination of enterotoxicity and frequency of o groups and k 88 antigen in strains from 200 piglets with neonatal diarrhoea. 1978369239
[cerebrospinal angiopathy after experimental protracted shock (author's transl)]. 1978371211
[evaluation of the efficacy of carbadox as a growth stimulant by microbiological criteria]. 1979375628
immunological studies in mice with swine edema disease principle (neurotoxin). 1979382704
survival times of bacteria in liquid manure. 1977382807
the role of thromboxane a2 in endotoxin-induced aggregation of guinea-pig platelets in vitro.large concentrations of endotoxin lipopolysaccharides (lps) e. coli o127:b8 and e. coli o26:b6 were needed for induction of platelet aggregation in citrated platelet rich plasma (prp) from normal guinea-pigs. when guinea-pigs were actively immunized with lps e. coli o127:b8 their prp became more sensitive to the aggregatory effects of this type of lps. the increase in sensitivity was rather selective since the responses to adp and lps e. coli o26:b6 remained unchanged. in endotoxin-stimulated pr ...1979386740
aspartate transaminase from e. coli: amino acid sequences of the nh2-terminal 33 residues and chymotryptic pyridoxyl tetrapeptide. 1979387032
renal damage due to urinary tract infection.chronic pyelonephritis is a disease of childhood which is carried on into adult life. children under the age of 4 in whom uti, vesico-ureteric reflux and pyelotubular back-flow coexist appear to be particularly at risk of developing kidney damage. attempts to prevent kidney damage in adults by detection and treatment of covert uti are doomed to failure because persistent bacteriuria in the adult is a consequence of underlying abnormality of the urinary tract rather than its cause. our main hope ...1979380901
effect of circulating, maternally derived antibody on the development of a local immune response in the intestine of the neonatal pig. 1979378047
influence of o and k antigens on the surface properties of escherichia coli in relation to phagocytosis.strains of escherichia coli with different o and k antigens were investigated with respect to physicochemical surface characteristics and liability to phagocytosis. using two-phase partitioning analysis for the surface characterization, three main groups of strains emerged: group i (o1:k1, o2:k1, o3:k2ab) showing both smooth hydrophilic o antigens and negatively-charged k antigen which rendered the strains maximally resistant to phagocytosis. group ii (o55:k59, o111:k58) showed no acidic k antig ...1979375674
the ability of cholestyramine resin and other adsorbents to bind escherichia coli enterotoxins.several adsorbent materials were evaluated for their ability to bind escherichia coli enterotoxins. cholestyramine, a strong anion-exchange resin, bound the heat-labile and the heat-stable types of enterotoxin and reduced significantly their effects in some animal models. however, its efficacy in the treatment of diarrhoeic piglets appeared to be adversely affected by the presence of milk in the alimentary tract.1979390155
the binding of lps to the lymphocyte surface.bacterial lipopolysaccharide (lps) from escherichia coli labelled with tritium has been used to follow the binding of lps to lymphocytes. binding to cells rose to a maximum 2-7 min after addition of [3h]-lps, followed by loss of [3h]-lps from cells, reducing to about 10% of the peak level at 20-30 min. peripheral blood lymphocytes, mesenteric lymph node and thymus cells of the pig and cba, c3h/he and c3h/hej mouse spleen cells all bound [3h]-lps transiently at similar levels. it is concluded tha ...1979391698
changes in the intestinal flora of young pigs with postweaning diarrhea or edema disease. 1979393690
detection of iga anti-escherichia coli plasma cells in the intestine and salivary glands of pigs orally and locally infected with e. coli. 1978369316
duration of anti-adhesive and bactericidal activities of milk from vaccinated sows on escherichia coli o149 in the digestive tract of piglets during the nursing period.piglets were exposed orogastrically to escherichia coli to enable study of the duration of anti-adhesive and bactericidal activities of milk of sows vaccinated with a k88 enriched e coli vaccine. there was a marked increase in the number of the challenge strain in the digestive tract of weaned piglets of all ages (between 888 and 2144 per cent). in contrast, there was a decrease in their number (75 per cent) in the day-old colostrum-fed piglets. when the piglets were two weeks old milk was still ...1979396618
[immunobiological activity of various blood serum fractions from cattle and swine. the dynamics of ig in specific serum against colibacillosis in pigs].the level of the igm and igg agglutinating antibody specific hyperimmune serum against coli-bacteriosis and its fractions were studied. the serum was obtained at various stages of the preparation, hyperimmunization and exploitation of serum producing swine by using the 2-mercapto-ethanol test. it was established that serum of the total globulin, alpha-beta-globulin and gamma-globulin fractions contains igm and igg agglutinating antibodies against e. coli. these immunoglobulin classes are synthet ...1979396718
some biological characteristics of escherichia coli strains isolated from acute diarrhoeal diseases of infants.the aim of this study was to evaluate the methods of biological characterization of escherichia coli strains, in order to use them as screening tests in clinical microbiological laboratories. in two thirds of the 45 e. coli, isolated from acute intestinal infection of infants, different factors of virulence were found. the strains showed prevalently an enterotoxigenic character (66,6%), which was in correlation with the increased permeability (62,2%). adhesive properties were present in 20% of e ...1979396738
course of antibiotic sensitivities in escherichia coli and staphylococcus aureus from animals.in order to establish the evolution of resistance against the common antibiotics from the beginning of their use up to the present time, the sensitivity of escherichia coli isolated from the cecum of wild boars was determined. the mic's of e. coli from these animals in a zoological garden was examined for comparison. the samples from the wild boars were collected in five forests in the belgian ardennes. the mic's obtained (table 1) are comparable to the mean values given in the early literature ...1979397700
effect of heat stable and heat labile escherichia coli enterotoxins and cholera toxin in combination with theophylline on unidirectional sodium and chloride flux in the small intestine of weanling swine.the effect of heat stable and heat labile escherichia coli enterotoxins or cholera toxin in combination with theophylline on net water, sodium and chloride and unidirectional sodium and chloride fluxes was examined in acute isolated loops of jejunum of weanling swine. the effect of heat stable enterotoxin in combination with theophylline was determined in loops located in the proximal jejunum, while combinations of theophylline and either heat labile enterotoxin or cholera toxin were studied in ...1978356942
characterization of plasmids that encode for the k88 colonization antigen.k88 antigen, and important virulence factor in porcine enteropathogenic escherichia coli (eec), can be transferred along with the ability to ferment the trisaccharide raffinose (raf). the plasmids from a number of eec strains that encode these two properties were isolated and characterized. in most strains the k88 and raf genes were found on a single nonconjugative plasmid approximately 50 x 10(6) daltons in size. this plasmid core was conserved with only slight variation among the strains teste ...1978352948
septic shock in obstetrics and gynecology. pathophysiology. 1977407404
acrolein. 1978415230
[residues of antimicrobial materials in the effluents of livestock farms (author's transl)].forty water samples were taken from streams of three different regions of belgium, with intensive husbandry and examined for residual microbiological activity. in some samples, activity was detected against staphylococcus aureus; none of them were active against e. coli and c. albicans. the substances in the few positive samples were not identified. with this low activity and the small proportion of positive samples (12 out of 41) any influence on the water biosphere due to the methods of farmin ...1976766304
[study of the role of colicins in the formation of microbial association in experiments in chick embryos].experiments were conducted on chick embryos; conditions of formation of bacterial cenosis of shigella sonnei and e. coli were reproduced. the role of colicinogenia in the formation of bacterial cenosis was demonstrated. an accumulation of colicines in high titres (1: 5120- 1:2560) in the chick embryonic cavity was noted in combined or separate cultivation of colicinogenic shigellae and e. coli. an intergenera transmission of the col-factor was traced in experiments on chick embryos. the data obt ...1975766520
[changes in the virulence of colibacteria under the influence of various biological factors]. 1975766580
[animal tissue polynucleotide phosphorylase].localization, physico-chemical and catalytic properties and possible biological functions of polynucleotide phosphorylase (pnpase) from animal tissues are discussed. in animal tissue cells pnpase has multiple localization; the major amount of the enzyme is localized in the endoplasmic reticulum ribosomes. in the nuclei pnpase, similar to other endo- and exo-rnases participates in the processing of precursor molecules of mature forms of rna, whereas in the cytoplasm it is involved in the destruct ...1978350293
microbial iron-chelators and their action on klebsiella infections in the skin of guinea-pigs.preparations of catechols from ethyl acetate extracts of cultures of klebsiellae in a low-iron medium contained iron-chelators whose potency was measured by the reversal of the bacteristasis of escherichia coli and klebsiellae in unheated horse serum, and of the growth-inhibition of these two organisms by ethylene diamine di-orthohydroxyphenyl acetic acid (edda). as revealed by in situ tests of paper chromatograms, there was a multiplicity of biologically active chelators in the preparations. ca ...1978350255
purification and properties of a pig heart thiolase with broad chain length specificity and comparison of thiolases from pig heart and escherichia coli.a thiolase (acetyl coa acyltransferase, ec 2.3-1.16) which acts on substrates of various chain lengths (thiolase i) has been purified from pig heart muscle 366-fold to near homogeneity as judged by gel electrophoresis. its molecular weight was estimated to be 200,000 in the absence and 46,000 in the presence of sodium dodecyl sulfate. kinetic measurements with acetoacetyl coenzyme a, 3-ketohexanoyl-coa, 3-ketooctanoyl-coa, and 3-ketodecanoyl-coa yielded apparent km values of 16, 8.3, 2.4, and 1. ...1978344310
human milk and gut infection in the newborn. 1977336123
effect of heat stable and heat labile escherichia coli enterotoxins, cholera toxin and theophylline on unidirectional sodium and chloride fluxes in the proximal and distal jejunum of weanling swine.acute, isolated loops of proximal and distal jejunum of weanling swine were exposed to either heat stable porcine escherichia coli enterotoxin, heat labile porcine escherichia coli enterotoxin, cholera toxin or theophylline. unidirectional sodium fluxes in response to heat stable in the proximal jejunum were dependent on the length of time that the intestinal mucosae was exposed to the enterotoxin. net water, sodium and chloride and unidirectional sodium and chloride flux measurements in the pro ...1977332292
reduction of reactivity of escherichia coli enterotoxins by intestinal mucosal components.incubation studies involving rabbit and piglet small intestinal mucosal components and escherichia coli and vibrio cholerae enterotoxins were conducted at 37 and 4 degrees c. mucosal homogenate cytosol from rabbits did not significantly alter the reactivities of either cholera enterotoxin (ct) or e. coli labile enterotoxin (lt). however, mucosal homogenate cytosol from piglets was capable of neutralizing lt, though it had no effect on e. coli stable enterotoxin. lt became bound to piglet and rab ...1977326677
etiologic diagnosis of diarrheal disease of calves: frequency and methods for detecting enterotoxin and k99 antigen production by escherichia cola.escherichia coli isolated from calves in minnesota and montana were tested for enterotoxigenicity via bio-assay of cell-free broth culture fluid and for k99 antigen via a serum agglutination test. infant mice were used to assay for heat-stable enterotoxin (st), and adrenal cells in culture were used to assay for heat-labile enterotoxin (lt). forty-six of the 345 e coli isolates produced st enterotoxin, but none produced lt enterotoxin. thirty-five of the 46 enterotoxigenic isolates had k99 antig ...1976786085
[change in the virulence of flexner shigellae following transmission of several parts of chromosomes related to the synthesis of o-antigen lipopolysaccharide from e coli k-12 and r-mutants of shigellae]. 1976790821
[comparative study of the virulence of enteropathogenic intestinal bacilli 0124 and crimea]. 1977325332
escherichia coli strains isolated from diarrheic piglets in the province of quebec.during 1972 to 1974, 112 escherichia coli strains isolated from diarrheic piglets were recieved from different parts of the province of quebec, canada. fifty-six strains elicited a positive gut loop response in three week old piglets and were then considered as moon's class 1 enteropathogens, while four of the 56 remaining strains reacted only in ten day old piglets and were classified as class 2 enteropathogens. forty-eight strains produced both a heat-labile and a heat-stable enterotoxin, whil ...1976793691
characterization of enterotoxigenic bovine escherichia coli.among 300 isolates of bovine escherichia coli, 56 which had been found enterotoxigenic in calf gut loops were characterized on the basis of o and k antigens, colonial morphology and resistance to seven antimicrobial drugs. the 56 isolates enterotoxigenic in the calf were compared with the nonenterotoxigenic ones. of the 56 enterotoxigenic e. coli the majority possessed the a type of k antigen and had ok groups, o9:k(ps274) or o101:k(rvc118). fourteen of these isolates had the k99 antigen. none o ...1976793694
the mutagenicity of cyclamates and their metabolites. 1976796692
[drug resistance and virulence of citrobacter isolated from the focus of piglet intoxication]. 1976797596
[experimental studies on the pathogenesis of coli enterotoxemia in swine. 3. effect of coli enterotoxin in weaned piglets following intragastric administration].the following results were obtained from experiments with 23 clinically intact weaned piglets, aged between five and six weeks (18 animals) or eight weeks (five animals), which had received three intragastric applications of crude enterotoxin (culture filtrate supernatant of escherichia coli o149:k91(b)k88(l):. 1. twelve animals (52 per cent) responded to enterotoxin application by temporary outbreaks of diarrhoea (1-8.5 hours) following differentiated latencies (2.5-10 hours). no diarrhoea was ...1976798558
[effect of acetylsalicylic acid on experimentally induced endotoxin reactions in swine].the time and severity of clinical responses to endotoxin depend on dosage, administration of acetylsalicyclic acid and indometacin prior to endotoxin application may delay or moderate the clinical phenomena that are likely to result from certain endotoxin amounts.1976798559
[actinomycin do inhibition of rna biosynthesis and its interaction with dna preparations].actinomycin d0 differing from actinomycin d in replacement of i residue of sarcosine by glycine in one of the peptide chains was found to have a lower inhibitory effect on rna synthesis in various biological systems. on interaction of e. coli with dna actinomycin d0 formed a complex with dna having the binding constant k=1.6 with 10-5 which was 100 times lower than the binding constant of actinomycin d. therefore, actinomycin d0 formed a complex with dna which was 100 times less stable than acti ...1975806255
in vitro study of the influence of virginiamycin and spiramycin on the composition and biochemical activities of the gastrointestinal flora of piglets. ii. influence on the biochemical activities of the microflora.chemical analysis was carried out on 54 in vitro fermentations to study the influence of nutritional doses of virginiamycin and spiramycin on the metabolic activity of the gastro-intestinal flora of 18 in vivo treated piglets. changes in the decomposition of glucose and the production of nh3, volatile acids and lactic acid are recorded in function of time. these results are in good accordance with changes noted in the composition of the bacterial flora, such as a marked decrease in the productio ...1975808048
[false-positive results obtained on examining slaughtered animals for the presence of antibiotic residues (author's transl)].as part of the examination of emergency-slaughtered animals for the presence of antibiotic residues, studies were done to see whether false-positive results would be obtained when the sarcina lutea kidney test and bacillus subtilis bga test were performed. when the s. lutea kidney test was positive in cattle, calves and swine, penicillin was invariably found to be present in those animals, the histories of which showed that they had not been given antibiotics. a syringe and an injected fluid con ...1975809859
the in vivo opsonizing activity of fab mu and f(c)5 mu fragments of pig immunoglobulin m to escherichia coli 0 55 in newborn, precolostral piglets. 1975812799
interference by neisseria gonorrhoeae growth by other bacterial species.growth of neisseria gonorrhoeae from clinical specimens has been enhanced by the use of selective media that inhibit the simultaneous growth of other microorganisms. one explanation for this enhancement could be that certain other bacteria inhibit gonococcal growth. this hypothesis was examined by testing 167 bacterial isolates for in vitro gonococcal inhibition; 34.1% of the isolates failed to inhibit the gonococcus, but 12.0% produced weak inhibition and 53.9% strongly inhibited n. gonorrhoeae ...1976823175
[influence of cleansing and disinfection of drinking troughs on germ content and coli titer of drinking water]. 1977319787
a cyclic adenosine 3',5'-monophosphate-dependent histone kinase from pig brain. purification and some properties of the enzyme.a cyclic adenosine 3',5'-monophosphate-dependent histone kinase (atp: protein phosphotransferase, ec 2.7.1.37) was isolated from pig brain. the enzyme has been purified 1140-fold; it is homogeneous on polyacrylamide gel electrophoresis and gel filtration. the estimated molecular weight of the enzyme is 120 000. histone kinase dissociates into a catalytic subunit and a regulatory one (molecular weights 40 000 and 90 000, respectively). the catalytic subunit has been obtained in homogeneous state ...1975235302
[pathogenesis of toxin caused gastroenteritis of vibrios and coli bacteria]. 19751089519
evidence for bactericidal activity of polymorphonuclear leukocytes without phagocytosis.the relationship between phagocytosis and bactericidal action of polymorphonuclear leukocytes was examined by comparing the functions of cytochalasin d-treated leukocytes with those of the control. measurement of phagocytotic and bacterial dna-degrading activities using escherichia coli prelabeled with [3h]thymidine revealed that phagocytosis and bacterial dna degradation were inhibited by treatment with cytochalasin d to about 50 and 10% of the control, respectively. nevertheless, the bacterici ...1979225308
[bacteriocinogeny in yersinia enterocolitica].bacteriocinogeny was found in 19.8 per cent of y. enterocolitica strains tested. it was designated by the authors as enterocoliticacinogeny and the substance produced by the pathogen was named enterocoliticacine. bacteriocinogeny was found among the collection strains isolated in the south primorye and not among the strains isolated abroad. some cultures of y. enterocolitica isolated abroad and in the south primorye were used as indicator strains for revealing enterocoliticacinogeny. enterocolit ...19751091204
enzymatic arginylation of beta-melanocyte-stimulating hormone and of angiotensin ii.porcine beta-melanocyte-stimulating hormone and angiotensin ii were examined as acceptors in the reaction catalyzed by arginyl-trna-protein transferase. both inhibited enzymatic transfer of [14c]arginine from trna to bovine albumin. inhibition was competitive with albumin and the k-i values were, respectively, 15 and 0.8 mum. the expected arginylated compounds were isolated and characterized. beta-melanocyte-stimulating hormone and its arginylated product had identical activities in the frog epi ...19751091639
preweaning mortality in the pig. pathological findings in piglets dying between birth and weaning.during a survey of preweaning mortality 538 piglets which died between birth and weaning were autopsied. the results of laboratory examinations permitted a division of the findings into a number of syndromes which were considered to be associated with the immediate cause of death. the non-infectious conditions such as trauma, starvation and suffocation were the most common. seventy-eight piglets were included in the trauma group because of depressed cranial bone fractures or visceral rupture lea ...1977200212
some immunological aspects of liver function. 19751093339
gnotobiotic pigs-derivation and rearing.the procurement, rearing, nutrition and microbiological monitoring of gnotobiotic pigs and a method for conditioning of primary, colostrum-deprived, specific pathogen free pigs is described. as compared to the established hysterectomy and closed hysterotomy methods for the derivation of gnotobiotic piglets an alternative approach, open caesarian section with the sow maintained under general halothane-nitrous oxide anaesthesia and the introduction of each fetus into the sterile isolator via a liq ...1978154359
mechanisms of immunity in bacterial infections. 197995156
relationships of antibiotics in animal feeds and salmonellosis in animals and man. 19751095537
the effects of feeding tetracycline, nitrovin and quindoxin on the drug-resistance of coli-aerogenes bacteria from calves and pigs. 19751095540
Displaying items 1 - 100 of 1980