| a study of swine dysentery by immunofluorescence and histology. | twenty-six specific-pathogen-free pigs were fed pure cultures of treponema hyodysenteriae. five untreated pigs were controls. distribution of this large spirochete in pigs with swine dysentery was shown by the indirect fluorescent antibody technique. findings by this method were compared with those from dark-field examination of colonic mucosal scrapings and from tissue sections. the cultures caused mucohemorrhagic colitis which by 10 days after inoculation was indistinguishable from the colitis ... | 1977 | 335630 |
| pathological observation on experimental swine dysentery. | experimental swine dysentery caused by 4 cultured strains (s73/2, dj183, dj70 and dk762) of treponema hyodysenteriae was studied pathologically. the distribution and quantity of treponemes were examined on tissue sections stained by the warthin-starry method. of the organs the colon contained the largest number of treponemes and the cecum and rectum the second largest number. histopathological lesions were restricted to the large intestine. they ranged from mild catarrhal colitis in the mild cas ... | 1979 | 522890 |
| application and evaluation of enzyme-linked immunosorbent assay and immunoblotting for detection of antibodies to treponema hyodysenteriae in swine. | an enzyme-linked immunoassay (elisa) has been developed to detect serum immunoglobulin antibodies g and m to treponema hyodysenteriae in vaccinated, experimentally infected and naturally infected swine. naturally infected swine gave elisa titres that were similar to experimentally infected swine, but were significantly less than the titres of vaccinated swine. when serum from naturally infected swine was used to probe nitrocellulose blots of sodium dodecyl sulphate-polyacrylamide gel electrophor ... | 1991 | 1936151 |
| autoclaved liquid medium for propagation of treponema hyodysenteriae. | three liquid media that differ slightly in composition but not in the method of preparation were developed for the propagation of treponema hyodysenteriae and treponema innocens. the three media are unique in that all components are sterilized by autoclaving before use. these media supported better growth of t. hyodysenteriae than did previously used liquid media. | 1986 | 3771757 |
| swine dysentery. ii. characterization of lesions in pigs inoculated with treponema hyodysenteriae in pure and mixed culture. | | 1972 | 4480858 |
| electron microscopy studies of human intestinal spirochetes. | the ultrastructure of twenty human intestinal spirochetes was analyzed using the electron microscope. negatively stained cells were generally found to be loosely and irregularly waved. the isolates had cell dimensions ranging from 0.18-0.35 micron in width and from 3.9-14.2 micron in length. twin bundles of flagella were present in the space between the cytoplasmic membrane and the outer membrane. the majority of isolates had five flagella inserted sub-terminally at each cell end. human intestin ... | 1987 | 3609249 |
| evaluation of selective media for primary isolation of treponema hyodysenteriae and treponema innocens. | a total of 2450 samples of feces, intestinal contents and colon mucosal scrapings were bacteriologically examined. a total of 53 strains of treponema sp. were isolated, and 45 strains of bacteroides sp., 30 strains of e. coli, 30 strains of micrococcus sp. and 10 strains of streptococcus d isolates were randomly selected. growth promoting studies showed statistically significant stimulation of treponema sp. growth by yeast extract, chicken egg yolk and rumen fluid. different growth inhibitors we ... | 1986 | 3533408 |
| characterization of the attachment of treponema hyodysenteriae to henle intestinal epithelial cells in vitro. | properties of the attachment of treponema hyodysenteriae to henle intestinal epithelial (hie 407) cells were examined. the frequency of attachment depended on the motility and viability of the spirochetes. rabbit hyperimmune and swine convalescent antisera inhibited attachment. treatment of hie cells with neuraminidase had no effect on attachment; however, treatment of spirochetes with the enzyme decreased adherence significantly (p = 0.01). attachment was inhibited by n-acetylneuraminic acid, d ... | 1989 | 2802317 |
| comparison of the genomes of pathogenic treponemes of human and animal origin. | the aim of this study was to compare the genomes of two strains of intestinal treponemes, which were isolated from patients suffering from intestinal disorders, with that of treponema hyodysenteriae, the known etiological agent of swine dysentery (bloody scours). the guanine-plus-cytosine contents of the three dnas were found to be 28.5 to 30.0%. dna-dna hybridization in liquid phase indicated a high degree of homology (56 to 95%) among the human strains and with t. hyodysenteriae. one of the hu ... | 1989 | 2707863 |
| antibodies to a common outer envelope antigen of treponema hyodysenteriae with antibacterial activity. | outer envelopes of treponema hyodysenteriae strains p18a and vs1 were prepared and characterized by sds-page. in western blot analysis of eleven strains of t. hyodysenteriae and two intestinal non-pathogenic spirochaetes, polyclonal antiserum raised to the outer envelopes of strain p18a contained antibodies primarily to two polypeptides. a 45 kda polypeptide was present in only two strains of t. hyodysenteriae, p18a and mc52/80, whereas another antigen of 16 kda was common to all eleven strains ... | 1989 | 2634083 |
| comparative analysis of the genomes of intestinal spirochetes of human and animal origin. | the aim of the present work was to compare the genomes of 21 strains of intestinal spirochetes, which were isolated from patients suffering intestinal disorders, with those of treponema hyodysenteriae (strain p18), the known etiological agent of swine dysentery (bloody scours), and of a nonpathogenic strain (m1) of treponema innocens. the percent guanine-plus-cytosine value of the 23 dnas was found to be 25.5 to 30.1, as determined by a double-labeling procedure based on nick-translation by dna ... | 1989 | 2535832 |
| effect of treponema hyodysenteriae infection on mucosal mast cells and t cells in the murine cecum. | the pathogenic mechanisms responsible for the development of lesions in swine and mice after infection with treponema hyodysenteriae have not been fully characterized. the release of inflammatory mediators from mast cells has been postulated to play a role in lesion development during swine dysentery. therefore, c3h/hen mice were infected with t. hyodysenteriae, and mucosal mast cell (mmc) numbers were examined in cecal sections. an initial increase in mmc numbers from 13 to 22 per 50 crypt vill ... | 1990 | 2294059 |
| new serogroups of treponema hyodysenteriae (g, h and i). | | 1990 | 2126408 |
| novel method for measuring growth of treponema hyodysenteriae and its application for monitoring susceptibility of clinical isolates to antimicrobial agents. | a microdilution assay method was developed to determine mics of antibiotics for treponema hyodysenteriae and to estimate the bacterial burden of mice colonized by this swine pathogen. hemolysis of bovine erythrocytes in broth was correlated with growth of the spirochete, and growth inhibition by drugs could be determined by measuring hemolysis. mics of 10 antibiotics for eight clinical isolates of t. hyodysenteriae measured by this method were similar to values obtained by an agar dilution metho ... | 1991 | 1759821 |
| the isolation of a treponema hyodysenteriae-like organism associated with swine dysentery in norway. | | 1975 | 1180192 |
| passive hemolysis test for antibody to treponema hyodysenteriae. | swine were infected orally with pure cultures of treponema hyodysenteriae, and the humoral antibody response was measured by a passive hemolysis test (passive hemagglutination test with the use of complement). antibody to t. hyodysenteriae was detected as early as 1 week and later on at 4 months after exposure. however, peak serum titers were obtained after challenge at 4 weeks postinfection. the usefulness of the test as a potential diagnostic tool for antibody to t. hyodysenteriae is discussed ... | 1976 | 992870 |
| isolation of treponema hyodysenteriae from feces of pigs affected with swine dysentery by use of a medicated medium. | | 1977 | 854087 |
| factors affecting the survival of treponema hyodysenteriae in dysenteric pig faeces. | treponema hyodysenteriae was found to survive for periods of up to 48 days in dysenteric pig faeces stored at temperatures between 0 degree c and 10 degree c inclusive. survival was reduced to seven days at 25 degree c and did not exceed 24 hours at 37 degree c. dilution 1:10 with tapwater appeared to enhance survival to a maximum of 61 days at 5 degree c but further dilution reduced it. drying and exposure to disinfectants rapidly eliminated t hyodysenteriae from dysenteric faeces. phenolic and ... | 1978 | 685104 |
| swine dysentery: studies of gnotobiotic pigs inoculated with treponema hyodysenteriae, bacteroides vulgatus, and fusobacterium necrophorum. | transmission experiments were carried out in gnotobiotic pigs to determine whether lesions typical of swine dysentery could be produced by oral inoculation of treponema hyodysenteriae in combination with bacteroides vulgatus or fusobacterium necrophorum, or both. each of the organisms had been isolated from swine with early lesions of the disease. lesions were not found in 6 pigs inoculated with t hyodysenteriae alone, in 4 pigs given f necrophorum and t hyodysenteriae, or in 4 pigs given b vulg ... | 1978 | 624669 |
| isolation of treponema hyodysenteriae from sources other than swine. | fecal samples were collected from animals and environments on 3 swine farms and cultured for treponema hyodysenteriae. each farm was a farrow-to-finish operation and, at the time of sampling, swine dysentery was enzootic among 8- to 22-week-old pigs. pathogenic t hyodysenteriae was isolated from pigs on all 3 farms. on farm a, nonpathogenic t hyodysenteriae was isolated from a sample of lagoon water. on farm b, pathogenic t hyodysenteriae was isolated from a waste-holding pit. on farm c, a dog w ... | 1978 | 624667 |
| experimental infection of rabbit ligated ileal loops with treponema hyodysenteriae. | an in vivo animal model was used to assess the enteropathogenicity of the etiological agent (treponema hyodysenteriae) of swine dysentery. multiple ligated ileal loops, prepared in new zealand white rabbits, were challenged with either pathogenic (b78 and b204) or nonpathogenic (pu) isolates of the organism. the pathogenic isolates induced the onset of intestinal fluid accumulation as early as 4 h, with maximal fluid induction at 18 h postchallenge. gross lesions of the intestinal mucosa, observ ... | 1979 | 575109 |
| studies on the pathogenesis of swine dysentery. ii. search for a cytotoxin in spirochetal broth cultures and colon content. | broth cultures of treponema hyodysenteriae and colonic content from pigs with swine dysentery were tested for cytotoxicity in cell cultures, erythrocyte suspensions and in ligated segments of pig colon. live cells of t. hyodysenteriae attached to the surface of cells in all cultures tested but did not penetrate them nor cause morphologic change detectable by light microscopy. only live t. hyodysenteriae caused erythrolysis. broth cultures or colonic content sterilized by filtration or by disrupt ... | 1979 | 473487 |
| prevalence of treponema hyodysenteriae in healthy pigs. | | 1979 | 389007 |
| evaluation of microagglutination test for differentiation between serpulina (treponema) hyodysenteriae and s. innocens and serotyping of s. hyodysenteriae. | swine dysentery is a mucohemorrhagic diarrheal disease caused by the anaerobic spirochete serpulina hyodysenteriae. at present, the serotyping is done by immunodiffusion testing with lipopolysaccharide (lps) extract as antigen and rabbit hyperimmune sera produced against different serotypes of s. hyodysenteriae. since the preparation of lps is time-consuming and requires a large quantity of bacteria, it is desirable to use a serotyping method which does not require the extraction of lps. in the ... | 1994 | 7989552 |
| characterization of predominant bacteria from the colons of normal and dysenteric pigs. | bacterial populations adherent to the mucosa of the proximal colons of weaned, healthy pigs were compared with populations from pigs with dysentery induced by inoculation with a culture of treponema hyodysenteriae. isolates (136) representative of the predominant flora adherent to colonic epithelia of normal pigs and isolates (162) from pigs with dysentery were cultured anaerobically on a rumen fluid-based medium and characterized. most (71%) of the isolates from colonic epithelia of normal pigs ... | 1984 | 6508310 |
| detecting anti-treponema hyodysenteriae antibodies in swine serum using immunofluorometry. | an indirect fluorescent antibody test was adapted for measuring serum anti-treponema hyodysenteriae antibodies with a fluorometer. the immunofluorescence was recorded as fluorescent signal units. cultures of t. hyodysenteriae and treponema innocens were used as antigen. there was a significant (p less than 0.01) correlation between the immunofluorescence recorded with the fluorometer and that evaluated visually with a microscope. the swine exposed orally to swine dysentery infective inoculum and ... | 1983 | 6357409 |
| [pathogenesis of swine dysentery caused by treponema hyodysenteriae]. | | 1983 | 6354680 |
| characterization of two dna probes specific for serpulina hyodysenteriae. | two dna probes, one 1.1- and one 0.75-kb probe, specific for serpulina hyodysenteriae were isolated from a genomic library generated from virulent s. hyodysenteriae 5380. these probes are highly specific and react with all s. hyodysenteriae strains tested. under stringent conditions, the dna probes did not react with the nonpathogenic species serpulina innocens or with other species of enteric bacteria, including escherichia coli. both probes are able to detect s. hyodysenteriae in colony blot h ... | 1993 | 8349750 |
| bactericidal effect of normal swine sera on treponema hyodysenteriae. | treponema hyodysenteriae was incubated in 20% normal swine sera (nss) at 37 degrees c for 4 h, and viability was determined by a plate dilution method. nss was bactericidal for nonpathogenic t. innocens and avirulent t. hyodysenteriae, but not for virulent t. hyodysenteriae isolates. heat inactivation at 56 degrees c for 30 min, treatment with edta or egta [ethylene glycol-bis(beta-aminoethyl ether)-n,n-tetraacetic acid], or removal of immunoglobulin m eliminated the bactericidal activity of nss ... | 1986 | 3940997 |
| survival of treponema hyodysenteriae in samples of dysenteric pig faeces sent by post and stored at room temperature. | | 1985 | 3883637 |
| erythrocytes as a source of essential lipids for treponema hyodysenteriae. | mammalian erythrocytes were tested as a nutrient source for the spirochete treponema hyodysenteriae, the etiologic agent of swine dysentery. brain heart infusion (bhi) broth did not support growth of t. hyodysenteriae b204 or b78. however, bhi broth supplemented with washed bovine erythrocytes, erythrocyte membranes, or cholesterol was an excellent culture medium for these strains (4 x 10(8) to 10(9) cells per ml, final population densities). small amounts of cholesterol (3 to 10 micrograms/ml o ... | 1987 | 3804437 |
| 3-acetyl-4''-isovaleryl tylosin for prevention of swine dysentery. | the 21 field isolates of treponema hyodysenteriae which were tested were sensitive to 3-acetyl-4''-isovaleryl tylosin (aiv); the minimal inhibitory concentration was 0.25 to 16 micrograms/ml. 3-acetyl-4''-isovaleryl tylosin administered prophylactically to pigs at concentrations of 5 to 100 mg/kg of feed and tylosin at 110 mg/kg of feed for 28 or 31 days prevented swine dysentery induced by tylosin-sensitive t hyodysenteriae strain sq2; 15 nonmedicated, inoculated control pigs had bloody diarrhe ... | 1986 | 3789492 |
| cell membrane permeability and mitochondrial dysfunction-inducing activities in cell-free supernatants from serpulina hyodysenteriae serotypes 1 and 2. | membrane permeability (mp) and mitochondrial dysfunction-inducing (mdi) activities were detected in cell-free supernatants (cfs) of serpulina hyodysenteriae, using either hemoglobin release from porcine red blood cells (rbc) or cytoplasmic lactate dehydrogenase release from porcine peripheral blood lymphocytes (pbl), and reduction of the 3-(4,5-dimethylthiazoyl-2-y1)-2,5-diphenyltetrazolium bromide dye by porcine pbl. the mp and mdi activities of cfs correlated with each other for serotype 1 and ... | 1996 | 8800549 |
| swine dysentery: protection against experimental challenge following single dose parenteral immunisation with inactivated treponema hyodysenteriae. | groups of five pigs were vaccinated at three to four weeks old with either formolised treponema hyodysenteriae in oil adjuvant alone, formolised t hyodysenteriae in oil adjuvant plus formolised campylobacter coli in oil adjuvant, or sterile medium in oil adjuvant (as a control). each group was challenged four weeks after vaccination by oral dosing on two consecutive occasions with pure cultures of the homologous strain of t hyodysenteriae plus direct contact with two pigs exhibiting severe swine ... | 1983 | 6635345 |
| sensitivity to dimetridazole of field isolates of treponema hyodysenteriae. | | 1983 | 6836884 |
| dual flaa1 flab1 mutant of serpulina hyodysenteriae expressing periplasmic flagella is severely attenuated in a murine model of swine dysentery. | the motility imparted by the periplasmic flagella (pf) of serpulina hyodysenteriae is thought to play a pivotal role in the enteropathogenicity of this spirochete. the complex pf are composed of multiple class a and class b polypeptides. isogenic strains containing specifically disrupted flaal or flab1 alleles remain capable of expressing pf, although such mutants display aberrant motility in vitro. to further examine the role that these proteins play in the maintenance of periplasmic flagellar ... | 1996 | 8926083 |
| enzyme-linked immunosorbent assay for detection of antibody to treponema hyodysenteriae antigens. | the enzyme-linked immunosorbent assay (elisa) was evaluated and compared with the microtitration agglutination test for the detection of swine antibody to treponema hyodysenteriae lipopolysaccharide antigens. cells of t. hyodysenteriae serotypes 1 and 2 were extracted with hot phenol-water (68 degrees c). the lipopolysaccharide fraction from the aqueous phase was coated on plastic wells at concentrations of 1 micrograms (serotype 1) and 10 micrograms (serotype 2) of carbohydrate per ml. the elis ... | 1982 | 7040447 |
| investigation of a hemolysin produced by enteropathogenic treponema hyodysenteriae. | a hemolysin produced by treponema hyodysenteriae, the etiological agent of swine dysentery, was investigated. a virulent isolate (b204) was inoculated into a standard culture medium consisting of trypticase soy broth without dextrose (bbl microbiology systems) supplemented with 10% fetal calf serum in an atmosphere of 70:30 deoxygenated h2-co2. sterile cell-free filtrates were prepared at 2-h intervals and assayed for hemolytic activity by using washed sheep erythrocytes. the maximum hemolytic t ... | 1981 | 7216445 |
| effects of aflatoxin consumption on the clinical course of swine dysentery. | specific-pathogen-free pigs were examined for susceptibility to swine dysentery after daily treatment with aflatoxin b1 (0.07 to 0.14 mg/kg). in the pigs (group ii) given both aflatoxin and treponema hyodysenteriae, the incubation period before the onset of swine dysentery was shorter than that in pigs (group iii) given t hyodysenteriae alone. also, the number of days in which infected pigs showed signs of diarrhea and dysentery was greater in the group given aflatoxin and t hyodysenteriae than ... | 1981 | 7271036 |
| induction of swine dysentery in swine by the intravenous injection of filtered treponema hyodysenteriae. | swine dysentery was induced in 18 swine exposed by intravenous injection of a filtrate which contained treponema hyodysenteriae and was obtained from macerated colonic scrapings of swine dysentery. however, swine dysentery did not develop in swine injected intravenously with a pure culture of t. hyodysenteriae or when combined with a colonic filtrate from normal swine. diarrheal feces from the swine injected intravenously with the filtered t. hyodysenteriae contained more mucus, and fecal smears ... | 1981 | 7337868 |
| selective medium for the isolation of treponema hyodysenteriae. | | 1981 | 7340069 |
| studies on the properties of treponema hyodysenteriae with special regard to its systematic position. | | 1981 | 7342737 |
| comparison of selective culture and serologic agglutination of treponema hyodysenteriae for diagnosis of swine dysentery. | samples of faeces and serum were collected from pigs of various ages on 21 farms. faecal samples were cultured on trypticase soy agar containing 5% citrated bovine blood and 400 microgram per ml spectinomycin, incubated at 42 degrees c in gaspak jars under an atmosphere of 80% hydrogen: 20% carbon dioxide. antibody titres to treponema hyodysenteriae were determined by a microtitration agglutination method using merthiolate-inactivated whole cell antigen prepared from a beta- haemolytic isolate. ... | 1980 | 7361391 |
| experimental transmission of treponema hyodysenteriae from mice to pigs. | transmission experiments with treponema hyodysenteriae were conducted with mice and pigs, using laboratory mice as the carrier host. mice were intragastrically inoculated with t hyodysenteriae and placed in a contact with nonexposed mice or healthy pigs. contact mice mingled with infected mice at 5 or 180 days after inoculation were shedding t hyodysenteriae in their feces 9 to 14 days after exposure. pigs exposed directly to infected mouse feces were shedding t hyodysenteriae in their feces aft ... | 1980 | 7447116 |
| production and characterization of monoclonal antibodies against serpulina hyodysenteriae and s. innocens and their use in serotyping. | murine monoclonal antibodies (mabs) directed against serotypes 1, 2, 8, and 9 of serpulina hyodysenteriae and strain b256 of serpulina innocens were produced and characterized. a serological classification of 96 field strains of s. hyodysenteriae and 28 field strains of s. innocens isolated from pigs showing clinical signs of swine dysentery was performed by rapid dot enzyme-linked immunosorbent assay (elisa) with the mabs. the results indicated that the majority of the field strains of s. hyody ... | 1995 | 7494066 |
| production and characterization of monoclonal antibodies specific for lipooligosaccharide of serpulina hyodysenteriae. | serpulina (treponema) hyodysenteriae is the causative agent of swine dysentery, a contagious mucohemorrhagic disease of the colon. diagnosis of swine dysentery is extremely difficult because of the presence of cross-reactive antibodies to the proteins of s. hyodysenteriae and serpulina innocens, a nonpathogenic inhabitant of the porcine large intestine. therefore, monoclonal antibodies (mabs) against the serotype-specific lipooligosaccharide (los) antigens of s. hyodysenteriae were produced to r ... | 1995 | 7559965 |
| growth of serpulina (treponema) hyodysenteriae under iron-restricted conditions. | reference strains of serpulina hyodysenteriae expressed at least three iron-regulated proteins with apparent molecular masses of > 200, 134, and 109 kda when grown under iron-restricted conditions. cells of s. hyodysenteriae grown under these conditions also showed increased outer membrane bleb formation when examined by electron microscopy after negative staining. s. hyodysenteriae did not use the 2 most common types of siderophore, namely catechol and hydroxamate. western blotting with serum f ... | 1995 | 7648528 |
| susceptibility of porcine ileal enterocytes to the cytotoxin of serpulina hyodysenteriae and the resolution of the epithelial lesions: an electron microscopic study. | the cytotoxin from serpulina hyodysenteriae was injected into ileal loops of eight germ-free pigs, and the effects on the villi were observed after 1, 3, and 18 hours of exposure. the mature vacuolated villus enterocytes of the proximal part of the absorptive villi were most susceptible to the lethal effects of the cytotoxin and were extensively exfoliated. the enterocytes at the base of the villi, the goblet cells, and the follicle-associated epithelium of the dome villi, particularly the m cel ... | 1995 | 7725595 |
| prophylactic effect of dietary zinc in a laboratory mouse model of swine dysentery. | reduced prevalence of diarrhea and mortality has been reported after dietary supplementation with zinc compounds in swine with naturally acquired colibacillosis and those challenge-exposed with serpulina hyodysenteriae; however, the usefulness of this approach for control of enteric diseases of swine remains to be determined. to examine the effect of dietary zinc-containing compounds on the colonization and development of cecal lesions associated with s hyodysenteriae infection, a defined diet a ... | 1995 | 7771701 |
| use of commercial enzyme kits and fatty acid production for the identification of serpulina hyodysenteriae: a potential misdiagnosis. | the accuracy of identification of serpulina hyodysenteriae strains grown in a complex medium was 90% when 2 commercial test kits were used. unlike the other s. hyodysenteriae strains, s. hyodysenteriae strain p35/2 was unusual in being indole negative. the nonpathogenic intestinal spirochete pws/a, which is from a different species, was indole positive and alpha-galactosidase negative. identification of these spirochetes on the basis of these kits alone would have been incorrect. the analysis of ... | 1995 | 7779972 |
| reduced virulence of serpulina hyodysenteriae hemolysin-negative mutants in pigs and their potential to protect pigs against challenge with a virulent strain. | the role of the serpulina hyodysenteriae hemolysin encoded by the tlya gene in the pathogenesis of swine dysentery (sd) was studied. tlya mutants of two s. hyodysenteriae strains (b204 and c5) were tested for virulence in pigs. none of the animals developed sd. however, after infection with wild-type strain b204 or c5, the incidence of sd was 100 or 60%, respectively. thus, the tlya-encoded hemolysin of s. hyodysenteriae is an important virulence factor in sd. the potential of these mutants to p ... | 1994 | 8188345 |
| pathogenicity of porcine intestinal spirochetes in gnotobiotic pigs. | twelve intestinal spirochete strains of porcine origin were characterized on the basis of their phenotypic properties, by multilocus enzyme electrophoresis, and by pathogenicity testing in gnotobiotic pigs. the spirochetes used included two strains of serpulina hyodysenteriae (b204 and p18a), two strains of serpulina innocens (b256 and 4/71), one strain from the proposed new genus and species "anguillina coli" (p43/6/78), and seven non-s. hyodysenteriae strains recently isolated from united king ... | 1994 | 8188364 |
| a novel method for isolation of brachyspira (serpulina) hyodysenteriae from pigs with swine dysentery in italy. | brachyspira (serpulina) hyodysenteriae was isolated from 10 of 11 pigs with clinically suspected swine dysentery in six herds in northern italy. all strains were successfully isolated in the selective blood agar modified medium with spectinomycin and rifampin (bam-sr) currently used in our laboratory to isolate b. (s.) pilosicoli of human origin, after pre-treatment of intestinal material with spectinomycin and rifampin in foetal calf serum. isolates had phenotypic characteristics typical of b. ... | 2001 | 11278122 |
| identification of a novel group of serpulina hyodysenteriae isolates by using a lipopolysaccharide-specific monoclonal antibody. | a monoclonal antibody to serpulina hyodysenteriae 8930 was produced and was used to probe pronase-treated cell lysates of s. hyodysenteriae isolates in immunblots. the results showed that the monoclonal antibody was specific for only five closely related s. hyodysenteriae isolates: 8930, 5380, 70a, rmit 88, and rmit 97. | 1993 | 8501237 |
| monitoring experimental swine dysentery: rectal swab blood test and serpulina (treponema) hyodysenteriae detection. | in two experiments, ten postweaning piglets were inoculated intragastrically with cultures of serpulina (treponema) hyodysenteriae strain b204 and were individually monitored for swine dysentery. eight control animals received sterile culture medium. between the day of inoculation and 35 days later, rectal swab samples were assayed for fecal blood by means of a commercial test kit and for s. hyodysenteriae cells by direct (phase contrast) microscopy and selective culture methods. optimal detecti ... | 1993 | 8506612 |
| phenotypical characterisation of intestinal spirochaetes isolated from pigs. | a combined evaluation of the phenotypical properties of five serpulina type or reference strains and 163 swedish isolates of spirochaetes from pigs and two from birds was made. the porcine isolates were collected from herds with a history of dysentery or severe diarrhoea and from herds chosen at random. on the basis of beta-haemolysis, indole production, hippurate hydrolysis, and alpha-galactosidase, alpha-glucosidase and beta-glucosidase activity, the isolates could be divided into four main gr ... | 1995 | 8525077 |
| pigs experimentally infected with serpulina hyodysenteriae can be protected from developing swine dysentery by feeding them a highly digestible diet. | weaner pigs (n = 72) were fed 1 of 4 diets. these were based on either cooked rice and animal protein, cooked rice and lupin, wheat and lupin, or wheat and animal protein. twenty-six of the pigs were slaughtered after 1 month. those fed the highly digestible cooked rice and animal protein diet had drier colonic contents and faeces, lighter large intestines, and the contents of their large intestines had increased ph values and decreased total vfa concentrations. the other 46 were orally challeng ... | 1996 | 8620913 |
| differentiation of intestinal spirochaetes by multilocus enzyme electrophoresis analysis and 16s rrna sequence comparisons. | multilocus enzyme electrophoresis (mee) analysis and comparisons of nearly complete 16s rrna gene sequences (1416 nucleotide positions) were used to evaluate phylogenetic relationships among serpulina hyodysenteriae strain b78t, s. innocens strain b256t, brachyspira aalborgi strain 513at, and eight uncharacterised strains of swine, avian, and human intestinal spirochaetes. from mee analysis, nine strains could be assigned to five groups containing other intestinal spirochaetes (genetic distances ... | 1996 | 8869502 |
| the incidence of swine dysentery in pigs can be reduced by feeding diets that limit the amount of fermentable substrate entering the large intestine. | two experiments were conducted to test the hypothesis that feeding diets which limit the amount of fermentable substrate entering the large intestine would protect pigs against experimental infection with serpulina hyodysenteriae, the causative agent of swine dysentery. experiment 1 examined the effect of grain processing (hammer milling vs. steam flaking) and grain type (barley, groats, corn, sorghum and wheat) on indices of fermentation in the large intestine and the incidence of swine dysente ... | 1996 | 8914966 |
| differences in lymphocyte subpopulations and cell counts before and after experimentally induced swine dysentery. | the aim of this study was to examine the levels of circulating leukocytes and lymphocyte subpopulations before and immediately after experimentally induced swine dysentery. twenty-one healthy crossbred pigs (approximately 22 kg) were orally inoculated with brachyspira hyodysenteriae. blood was sampled before inoculation and when clinical signs of swine dysentery occurred. pigs that remained healthy were sampled when killed. total and differential white blood cell counts were performed, and lymph ... | 2004 | 15017281 |
| induction of interleukin (il)-1beta and il-8 mrna expression in porcine macrophages by lipopolysaccharide from serpulina hyodysenteriae. | lipopolysaccharide (lps) is a classic inducer of inflammatory cytokines and is a key virulence factor for most gram-negative pathogens. the effect of phenol-water (lps) and butanol-water (endotoxin) extracts from serpulina hyodysenteriae on inflammatory cytokine mrna expression from porcine alveolar macrophages was investigated. the lps and endotoxin extracts from s. hyodysenteriae induced a dose-dependent expression of interleukin 1beta (il-1beta) and il-8 which was weak compared with the respo ... | 1996 | 8926114 |
| enhanced isolation of serpulina hyodysenteriae by using sliced agar media. | a method has been developed for separating serpulina hyodysenteriae, a large spirochete and the causative agent of swine dysentery (sd), from other fecal anaerobic bacteria in rectal and colonic swabs. this was done by cutting the blood agar in parallel cuts and streaking perpendicular to the cuts in the center of the petri dish. migration of s. hyodysenteriae from the central streak was apparent by the presence of strong beta-hemolysis along the edges of the cuts. if only s. hyodysenteriae migr ... | 1996 | 8940426 |
| immunomagnetic separation of the intestinal spirochaetes brachyspira pilosicoli and brachyspira hyodysenteriae from porcine faeces. | porcine intestinal spirochaetes are fastidious anaerobic organisms and, as a consequence, it has been necessary to develop various protocols to enhance their isolation from or detection in faeces. immunomagnetic separation (ims) is a method developed recently to improve separation of target cells from mixed cell suspensions. the purpose of the present study was to compare the relative sensitivity of ims for isolation of brachyspira pilosicoli and brachyspira hyodysenteriae with current routine d ... | 2004 | 15017286 |
| rapid identification of porcine serpulina species by colony blot assay using a genus-specific monoclonal antibody. | an immunoglobulin g monoclonal antibody (mab c9e8) recognising a genus-specific epitope on the 26 kda protein of porcine serpulina species organisms was used in a simple colony blot assay to detect serpulina in cultures grown directly on blood agar plates from pig faeces and tissues. the mab detected even a few colonies of the organism in the presence of an abundant growth of non-serpulina organisms. the whole procedure was completed in less than three hours. a total of 123 strains of s hyodysen ... | 1996 | 8961523 |
| rapid isolation of brachyspira hyodysenteriae and brachyspira pilosicoli from pigs. | the aim of this study was to compare and evaluate the time required to isolate brachyspira hyodysenteriae and brachyspira pilosicoli from porcine faeces. this was done using previously described selective media (spectinomycin) s400, (colistin, vancomycin and spectinomycin) cvs and (spectinomycin, vancomycin, colistin, spiramycin and rifampin with swine faecal extract) bj, compared with the method based on blood agar modified medium, with spectinomycin and rifampin (bam-sr), including a pre-treat ... | 2004 | 15708820 |
| multiplex polymerase chain reaction for simultaneous detection of lawsonia intracellularis, serpulina hyodysenteriae, and salmonellae in porcine intestinal specimens. | proliferative enteritis, swine dysentery, and porcine salmonellosis are the most common enteric bacterial diseases affecting pigs in the growing and finishing stages of production. currently, diagnoses of these diseases by standard cultural techniques of intestinal specimens can be laborious, time consuming, and expensive (swine dysentery, porcine salmonellosis) or impossible (proliferative enteritis). amplification by polymerase chain reaction (pcr) of dna sequences specific for each bacterial ... | 1997 | 9249167 |
| morphologic and temporal characterisation of lesions in an enhanced murine model of serpulina hyodysenteriae infection. | this laboratory has previously reported a murine model of serpulina hyodysenteriae infection in which mice fed a defined diet, teklad 85420 (td), developed caecal lesions more consistently than mice fed a conventional rodent chow (crc). the objectives of the current studies were to characterise and compare the time of onset of lesions, the morphological nature and severity of lesions and the extent of colonisation by s. hyodysenteriae in mice fed the two diets. in the first of two experiments, 5 ... | 1998 | 9511833 |
| investigations into field cases of porcine colitis with particular reference to infection with serpulina pilosicoli. | investigations into the possible causes of colitis and typhlocolitis were carried out on 85 pig units in the united kingdom between 1992 and 1996. serpulina pilosicoli was identified most commonly, occurring as the suggested primary agent on 21 (25 per cent) of the units but forming part of mixed infections on another 23 (27 per cent) of the units, the main co-infections being yersinia pseudotuberculosis (eight units), proliferative enteropathy (six units), salmonella species (four units) or ser ... | 1998 | 9549864 |
| detection of lawsonia intracellularis, serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, salmonella enterica, and haemolytic escherichia coli from swine herds with and without diarrhoea among growing pigs. | a polymerase chain reaction (pcr) was optimized to detect lawsonia intracellularis in faeces from naturally infected pigs. by combining a boiling procedure to extract dna and a nested pcr procedure, a detection limit at 2 x 10(2) bacterial cells per gram of faeces was achieved. the optimized pcr was used together with conventional culture techniques to detect serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes (wbhis), salmonella enterica, and haemolytic escherichia coli, in ... | 1998 | 9659692 |
| detection of extrachromosomal dna in italian isolates of weakly beta-haemolytic human intestinal spirochaetes. | weakly beta-haemolytic human intestinal spirochaetes (w beta his) isolated in italy showed an extrachromosomal band migrating at 4.3 kb after agarose gel electrophoresis of total dna. the band was observed within 4 strains (hrm2, hrm4, hrm7 and hrm14) among the 7 w beta his analysed and was resistant to proteinase k and rnase treatment, whereas after purification it was completely digested by incubation with dnasei. the origin, the structure and the significance of this extrachromosomal dna are ... | 1998 | 9699203 |
| immunological alterations during the clinical and recovery phases of experimental swine dysentery. | the aim of this study was to examine changes in the systemic immune response during the incubation period and following the onset of clinical swine dysentery, including the recovery period. ten healthy conventional pigs were inoculated with brachyspira hyodysenteriae. blood was sampled at pre-inoculation, at days 4 and 14 post-inoculation, during the first 4 days with clinical signs of dysentery and at days 1, 3, 7, 11 and 15 of the recovery period. eight pigs developed haemorrhagic diarrhoea. f ... | 2006 | 16772410 |
| [serpulina hyodysenteriae infections in swine]. | | 1998 | 9764463 |
| bacteriophages induced from weakly beta-haemolytic human intestinal spirochaetes by mitomycin c. | a comparative electron microscopic analysis of weakly beta-haemolytic spirochaetes related to human and animal intestinal spirochaetosis was done in order to search for the presence of inducible bacteriophages associated with these spirochaetes. bacteriophages were detected at the electron microscope after experimental induction with mitomycin c in 4 strains of weakly beta-haemolytic spirochaetes related to human intestinal spirochaetosis, in serpulina pilosicoli strain p43/6/78, the causative a ... | 1998 | 9871330 |
| treatment of swine dysentery--problems of antibiotic resistance and concurrent salmonellosis. | | 1998 | 9871960 |
| a comparison of the morphologic effects of serpulina hyodysenteriae or its beta-hemolysin on the murine cecal mucosa. | studies were carried out to compare the early morphologic changes in the cecal mucosa of mice either infected with serpulina hyodysenteriae or exposed to the beta-hemolysin of s. hyodysenteriae. sixty-five 12-24-week-old c3h/heouj mice were infected with s. hyodysenteriae by gastric intubation. two mice were necropsied every hour for 30 hours following infection. s. hyodysenteriae was isolated from the cecal contents of each mouse at all time points. macroscopic lesions were first apparent at 14 ... | 1999 | 10490209 |
| sequence characterization of two new members of a multi-gene family in serpulina hyodysenteriae (b204) with homology to a 39 kda surface exposed protein: vspc and d. | previous cloning and sequencing of clones from a genomic library constructed from serpulina hyodysenteriae b204 had identified a tandem pair of open reading frames, identified as vspa and vspb (variable surface protein) expected to encode proteins with homology to ( but not identical with) a 39 kda surface exposed membrane protein from this animal pathogen. additional screening of the genomic library was performed to retrieve the remainder of the vspb gene using new oligonucleotide probes based ... | 1999 | 10510046 |
| characterization of three putative serpulina hyodysenteriae hemolysins. | serpulina hyodysenteriae hemolysin is though to be an important virulence factor in swine dysentery. one gene, tlya, previously called tly, encoding a hemolysin in s. hyodysenteriae strain b204 has been characterized (muir et al. infect immun 1992; 60: 529-35). two other genes of s. hyodysenteria strain b204, designated tlyb and tlyc, encoding hemolytic proteins in escherichia coli strain dh5 alpha were cloned and sequenced. the tlyb and tlyc genes, when expressed in e. coli, encode heat-labile, ... | 1994 | 7968456 |
| chemotactic response to mucin by serpulina hyodysenteriae and other porcine spirochetes: potential role in intestinal colonization. | chemotaxis of porcine spirochetes towards a variety of mucins was measured quantitatively by a capillary method. a chemotaxis buffer consisting of 0.01 m potassium phosphate buffer (ph 7.0) and 0.2 mm l-cysteine hydrochloride was necessary for chemotaxis of spirochetes. the optimum incubation time and incubation temperature were 1 h and 40 degrees c, respectively. the mucin concentration also affected the chemotaxis observed, and a concentration of 1% (wt/vol) was near the optimum. virulent serp ... | 1994 | 8063433 |
| identification of the gene encoding bmpb, a 30 kda outer envelope lipoprotein of brachyspira (serpulina) hyodysenteriae, and immunogenicity of recombinant bmpb in mice and pigs. | a gene encoding a 30kda outer envelope protein of the intestinal spirochaete brachyspira (serpulina) hyodysenteriae, was cloned and expressed in escherichia coli strain xlolr. five phagemids containing dna inserts encoding the protein were established and one clone (psha) was sequenced. an 816bp hypothetical open reading frame (orf) was identified, with a potential ribosome binding site (aggag), and putative -10 (tataat) and -35 (ttgaaa) promoter regions upstream from the atg start of the orf. a ... | 2000 | 10973699 |
| risk factors for intestinal pathogens in danish finishing pig herds. | the objective of this investigation was to identify risk factors for infection with the intestinal bacteria: lawsonia intracellularis, brachyspira hyodysenteriae, serpulina intermedia, brachyspira innocens, brachyspira pilosicoli and swine-pathogenic escherichia coli (serogroups o138, o139, o141 and o149) in danish finishing pig herds.a total of 79 herds was randomly selected and visited during 1998. from each herd, 20 faecal samples were collected from individual pigs weighing 30-50 kg. in tota ... | 2001 | 11448502 |
| dietary conjugated linoleic acid modulates phenotype and effector functions of porcine cd8(+) lymphocytes. | in vivo vaccination and challenge studies have demonstrated that cd8(+) lymphocytes are essential for the development of cell-mediated protection against intracellular pathogens and neoplastic cells. depletion of peripheral blood cd8(+) cells interferes with clearance of viruses and intracellular fungi, induction of delayed type hypersensitivity responses and antitumoral activity. in contrast to humans or mice, porcine peripheral cd8(+) lymphocytes are characterized by a heterogeneous expression ... | 2001 | 11533281 |
| antimicrobial susceptibility testing of australian isolates of brachyspira hyodysenteriae using a new broth dilution method. | the antimicrobial susceptibilities of 76 field isolates of brachyspira hyodysenteriae from different states of australia were tested in a newly developed broth dilution procedure. the antimicrobial agents used were tiamulin, valnemulin, tylosin, erythromycin, lincomycin and clindamycin. the results from the broth dilution susceptibility testing of 39 of the isolates were compared with results obtained for the same isolates using the agar dilution method. amongst the isolates tested by broth dilu ... | 2002 | 11731165 |
| effect of highly fermentable dietary fiber on the development of swine dysentery and on pig performance in a "pure--culture challenge model". | this study tried to evaluate the effect of highly fermentable fiber on the incidence and severity of swine dysentery (sd) after experimental oral infection with pure cultures of brachyspira (b.) hyodysenteriae. forty eight growing pigs were allocated to two groups and treated until slaughter as follows: group 1 (n = 24): infected with b. hyodysenteriae and fed with a food containing 9.6% highly fermentable neutral detergent fiber. group 2 (n = 24): infected with b. hyodysenteriae and fed with a ... | 2002 | 11852682 |
| nutritional regulation of porcine bacterial-induced colitis by conjugated linoleic acid. | excessive intake of saturated fatty acids and/or linoleic acid favors the induction of an array of lipid mediators and cytokines enhancing inflammatory responses. conversely, dietary supplementation with (n-3) fatty acids or vitamin d ameliorates inflammation and autoimmune diseases. although it was well accepted that conjugated linoleic acid (cla) prevented diseases with a common inflammatory pathogenesis (i.e., cancer and atherosclerosis), no studies were available on the roles of cla in mucos ... | 2002 | 12097686 |
| development of a duplex pcr assay for detection of brachyspira hyodysenteriae and brachyspira pilosicoli in pig feces. | a duplex pcr (d-pcr) amplifying portions of the brachyspira hyodysenteriae nadh oxidase gene and the b. pilosicoli 16s rrna gene was developed and then tested on dna extracted from 178 porcine fecal samples. the feces also underwent anaerobic culture and species-specific pcrs. fecal extraction-d-pcr detected seven additional samples containing b. hyodysenteriae and five more containing b. pilosicoli. | 2003 | 12843096 |
| further characterization of porcine brachyspira hyodysenteriae isolates with decreased susceptibility to tiamulin. | brachyspira hyodysenteriae is the causative agent of swine dysentery, a severe diarrhoeal disease in pigs. few drugs are available to treat the disease, owing to both antimicrobial resistance and withdrawal of drugs authorized for use in pigs. tiamulin is the drug of choice in many countries, but isolates with decreased susceptibility have recently been reported. the mechanism of tiamulin resistance in b. hyodysenteriae is not known and this facet is essential to understand the dissemination of ... | 2004 | 15017283 |
| decreased susceptibility to tiamulin and valnemulin among czech isolates of brachyspira hyodysenteriae. | the agar dilution method was used to investigate the sensitivity to pleuromutilins of 100 isolates of brachyspira hyodysenteriae isolated from 63 pig farms between 1997 and 2001. in the period under investigation, mics to both tiamulin and valnemulin increased, with differences between the periods 1997-98 and 1999-2001 being statistically significant (p < 0.001 for tiamulin and p < 0.0001 for valnemulin). between 1997 and 2001, the mic50 and mic90 of tiamulin increased from 0.062 and 0.25 microg ... | 2004 | 15017284 |
| evaluating the risk of pathogen transmission from wild animals to domestic pigs in australia. | wild animals contribute to endemic infection in livestock as well as the introduction, reintroduction and maintenance of pathogens. the source of introduction of endemic diseases to a piggery is often unknown and the extent of wildlife contribution to such local spread is largely unexplored. the aim of the current study was to quantitatively assess the probability of domestic pigs being exposed to different pathogens from wild animals commonly found around commercial piggeries in australia. spec ... | 2016 | 26711303 |
| comparison of methods for antimicrobial susceptibility testing and mic values for pleuromutilin drugs for brachyspira hyodysenteriae isolated in germany. | in germany treatment of swine dysentery is hampered by brachyspira hyodysenteriae strains showing elevated mic values to the few antibiotics licensed. therefore, susceptibility testing of clinical isolates is an important service to the swine practitioner. this study compares the established agar dilution procedure for antimicrobial susceptibility testing of this fastidious anaerobe to the broth microdilution test newly developed [anim. health res. 2 (2001) 59; vet. microbiol. 84 (2002) 123; j. ... | 2004 | 15288924 |
| application of nox-restriction fragment length polymorphism for the differentiation of brachyspira intestinal spirochetes isolated from pigs and poultry in australia. | sixty-nine intestinal spirochetes isolated from pigs and poultry in eastern australia were selected to evaluate the effectiveness of a species-specific pcr-based restriction fragment length polymorphism (rflp) analysis of the brachyspira nox gene. for comparative purposes, all isolates were subjected to species-specific pcrs for the pathogenic species brachyspira hyodysenteriae and brachyspira pilosicoli, and selected isolates were examined further by sequence analysis of the nox and 16s ribosom ... | 2005 | 15825489 |
| the distribution of bmpb, a gene encoding a 29.7 kda lipoprotein with homology to metq, in brachyspira hyodysenteriae and related species. | the distribution of the bmpb gene encoding bmpb, a 29.7 kda outer membrane lipoprotein of the intestinal spirochaete brachyspira hyodysenteriae, was investigated. using pcr, the gene was detected in all the 48 strains of b. hyodysenteriae examined and in brachyspira innocens strain b256t, but not in 11 other strains of b. innocens nor in 42 strains of other brachyspira spp. the gene was sequenced from b. innocens strain b256t and from 11 strains of b. hyodysenteriae. the b. hyodysenteriae genes ... | 2005 | 15863284 |
| the prevalences of brachyspira spp. and lawsonia intracellularis in swedish piglet producing herds and wild boar population. | the aim of the present study was to survey the prevalences of the enteric pathogens brachyspira hyodysenteriae, brachyspira pilosicoli and lawsonia intracellularis in swedish growing pigs and in the swedish wild boar population and to relate these findings to clinical signs. the study included 105 randomly selected herds, constituting approximately one third of swedish herds with a herd size of >100 sows. the herds were located all over the country. in these herds, growth promoters were not used ... | 2005 | 16283917 |
| characterization of brachyspira hyodysenteriae isolates from korea. | this study was done to characterize diversity in 10 brachyspira hyodysenteriae isolates in korea. the isolates were compared with 14 well-characterized non-korean strains of various brachyspira species. all korean isolates showed strong beta haemolysis and had blunt cell ends with 7-14 periplasmic flagella. they produced indole, and did not ferment fructose. they were alpha-glucosidase positive and alpha-galatosidase negative using the apizym kit. using polyclonal antisera raised in rabbits agai ... | 2005 | 16293998 |
| differential expression of the bhmp39 major outer membrane proteins of brachyspira hyodysenteriae. | the enteric, anaerobic spirochete brachyspira hyodysenteriae is the causative agent of swine dysentery, a severe mucohemorrhagic diarrheal disease of pigs that has economic significance in every major pork-producing country. recent investigation into potential vaccine candidates has focused on the outer membrane proteins of b. hyodysenteriae. bhmp39 (formerly vsp39) is the most abundant surface-exposed outer membrane protein of b. hyodysenteriae; its predicted gene sequence has previously been s ... | 2006 | 16714554 |
| effects of griseoviridin and viridogrisein against swine dysentery in experimental infection by using mice and pigs. | griseoviridin, a known antibiotic produced by streptomyces cacaoi subsp. cacaoi, was found to be active against brachyspira hyodysenteriae--the bacterium causing swine dysentery. an in vitro synergism is observed when it is used in combination with viridogrisein--a simultaneously produced antibiotic. in mouse experiments, the effect of griseoviridin alone was less than that of lincomycin--a commercially available swine dysentery medication. however, a 1:1 mixture of griseoviridin and viridogrise ... | 2006 | 16820711 |
| the effect of fermentable carbohydrates on experimental swine dysentery and whip worm infections in pigs. | an experiment was conducted to study the effect of diets with contrasting fermentability in the large intestine on experimental infections with brachyspira hyodysenteriae, the causative agent of swine dysentery, and the whip worm, trichuris suis, in pigs. two diets with organically grown ingredients were composed. both diets were based on triticale and barley and supplemented with either rape seed cake (diet 1) or dried chicory root and sweet lupins (diet 2). the study had a three-factorial desi ... | 2007 | 17049759 |
| a novel enteropathogenic, strongly haemolytic spirochaete isolated from pig and mallard, provisionally designated 'brachyspira suanatina' sp. nov. | atypical, strongly haemolytic porcine isolates of intestinal spirochaetes differing genetically from brachyspira hyodysenteriae were identified and characterized. the isolates were subjected to culture and biochemical tests, antimicrobial susceptibility testing and molecular analyses. none of four species-specific polymerase chain reaction systems targeting genes of b. hyodysenteriae gave a positive reaction. all the atypical porcine isolates were identical in their partial 16s rrna and nox gene ... | 2007 | 17359270 |
| simultaneous detection of brachyspira hyodysenteriae, brachyspira pilosicoli and lawsonia intracellularis in porcine faeces and tissue samples by multiplex-pcr. | diarrhoea in growing and finishing pigs is usually caused by infectious agents and laboratory diagnosis is a prerequisite for efficient therapy. cultivation of brachyspira hyodysenteriae or brachyspira pilosicoli and detection of lawsonia intracellularis by means of immunofluorescence tests (ift) are time-consuming and in some cases lack sensitivity. a multiplex-pcr was designed to detect simultaneously these three pathogens in faeces and tissue samples, allowing the differential diagnosis of dy ... | 2007 | 17931231 |
| evaluation of recombinant bhlp29.7 as an elisa antigen for detecting pig herds with swine dysentery. | swine dysentery (sd) results from infection of the porcine large intestine with the anaerobic intestinal spirochaete brachyspira hyodysenteriae. diagnosis of sd traditionally has relied on detecting the spirochaete in the faeces of acutely affected pigs. to date simple and reliable serological assays that can be applied as a diagnostic tool at the herd level have not been available. in the current study a recombinant histidine tagged 29.7 kda lipoprotein of b. hyodysenteriae (his6-bhlp29.7) was ... | 2009 | 18619744 |
| isolation, oxygen sensitivity, and virulence of nadh oxidase mutants of the anaerobic spirochete brachyspira (serpulina) hyodysenteriae, etiologic agent of swine dysentery. | brachyspira (serpulina) hyodysenteriae, the etiologic agent of swine dysentery, uses the enzyme nadh oxidase to consume oxygen. to investigate possible roles for nadh oxidase in the growth and virulence of this anaerobic spirochete, mutant strains deficient in oxidase activity were isolated and characterized. the cloned nadh oxidase gene (nox; genbank accession no. u19610) on plasmid per218 was inactivated by replacing 321 bp of coding sequence with either a gene for chloramphenicol resistance ( ... | 1999 | 10543819 |