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an evaluation of serologic tests used to diagnose brucellosis in buffaloes (bubalus bubalis).five serologic procedures were evaluated in buffaloes and results compared with cultures of 64 udder secretion specimens. the rivanol test was superior to all other methods in specificity. the card, rivanol and complement fixation tests identified all infected cows. many sera had complement fixing antibodies while agglutinins were absent. it was concluded that these were false positive results. a new procedure, the particle concentration fluorescence immunoassay, was a sensitive screening test b ...19921306917
post-exposure serological and bacteriological responses of water buffalo (bubalus bubalis) to brucella abortus biovar 1 following vaccination with brucella abortus strain rb51.serological and bacteriological responses to brucella abortus biovar 1 following vaccination with b. abortus strain rb51 (rb51) were evaluated in thirty domestic water buffalo (bubalus bubalis) randomly divided into five treatment groups. groups i to v received, respectively, the recommended dose (rd) of rb51 vaccine once, rd twice 4 weeks apart, double rd once, double rd twice 4 weeks apart, and saline once (control). vaccination did not result in a serological response. experimental animals re ...200718293615
standardisation of an indirect enzyme-linked immunosorbent assay for the detection of brucella antibodies in milk from water buffalo (bubalus bubalis) in italy.an indirect enzyme-linked immunosorbent assay (elisa) was evaluated for the detection of brucella antibodies in milk from water buffalo (bubalus bubalis linnaeus, 1758). the test accuracy was evaluated on milk samples from the campania region in italy. a total of 100 negative samples were collected from 10 officially brucellosis-free herds in salerno province, while 30 positive samples were collected from 3 herds in caserta province, where animals gave positive results to the official tests and ...201121240852
improvement in the diagnosis of brucella abortus infections in naturally infected water buffaloes (bubalus bubalis) using an elisa with a protein-g-based indicator system.brucellosis caused by brucella abortus in domestic water buffaloes (bubalus bubalis) raised under the traditional system of husbandry in northern india was diagnosed using an enzyme-linked immunosorbent assays (elisa) with a protein-g-based indicator system (protein-g elisa). a total of 1,551 animals that are positive (n = 61), negative (n = 243), and suspected (n = 1,247) for brucellosis were examined. rose bengal test (rbt) was used to predict the disease, and accordingly, animals were dichoto ...201121442153
brucellosis in domestic water buffalo (bubalus bubalis) of trinidad and tobago with comparative epidemiology to cattle.the water buffalo is an important domestic animal worldwide, and the local buffalypso variety was developed in trinidad to have improved beef qualities. brucellosis was diagnosed in trinidad and tobago during 1998 in both cattle and domestic water buffalo (bubalus bubalis) populations. brucellosis in the latter species is caused by infection with brucella abortus, similar to bovine brucellosis. control of brucellosis is of paramount importance to preservation of the genetic diversity of these an ...201121479842
serological profile of buffalo (bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose bengal, 2-mercaptoethanol and complement fixation tests.the serological profiles of 21 female buffaloes vaccinated between 3 and 8 months of age using brucella abortus strain 19 (s19) were evaluated by rose bengal (rbt), 2-mercaptoethanol (2me) and complement fixation (cft) tests. the serum strains were collected in day zero, 15, 30, 45, 60th days and subsequently to each 30 months, until 720th day after vaccination. no animal showed reaction in day zero. in 15th day above 95% of animals revealed reaction in all tests. all the animals presented absen ...201222284623
serological and bacteriological responses of water buffalo (bubalus bubalis) vaccinated with two doses of brucella abortus strain rb51 vaccine.thirty-two water buffalo (bubalus bubalis) calves aged 6–10 months were used to evaluate serological responses to brucella abortus strain rb51 (rb51) vaccination in a dose-response study and to compare the use of two selective media for the isolation of rb51. the animals were randomly divided into three treatment groups. groups i-iii received the recommended vaccine dose (rd) twice 4 weeks apart, rd twice 18 weeks apart and saline once, respectively. lymph nodes were excised from the three group ...201222297422
pheno- and genotyping of brucella abortus biovar 5 isolated from a water buffalo (bubalus bubalis) fetus: first case reported in the americas.an isolate of brucella spp. from an aborted water buffalo (bubalus bubalis) fetus was characterized based on its pheno- and genotype. the phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with brucella a and m monospecific antisera. the genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence is711 in the brucell ...201425113673
genome sequences of 11 brucella abortus isolates from persistently infected italian regions.bovine brucellosis, typically caused by brucella abortus, has been eradicated from much of the developed world. however, the disease remains prevalent in southern italy, persisting as a public and livestock health concern. we report here the whole-genome sequences of 11 isolates from cattle (bos taurus) and water buffalo (bubalus bubalis) that are representative of the current genetic diversity of b. abortus lineages circulating in italy.201526679575
virulence of brucella abortus isolated from cattle and water buffalo.brucellosis has been documented in domestic water buffalo (bubalus bubalis) but published literature is limited despite the importance of this species in tropical agricultural systems. the objective of this study was to compare the virulence of brucella abortus isolates recovered from cattle and water buffalo. nineteen strains of b. abortus from cattle and domestic water buffalo in trinidad were intraperitoneally inoculated into balb/c mice. spleens were cultured for b. abortus and histopatholog ...201020972706
comparative study on responses of cattle and water buffalo (bubalus bubalis) to experimental inoculation of brucella abortus biovar 1 by the intraconjunctival route--a preliminary report.the preliminary study was conducted to assess the virulence of a strain of brucella abortus (1969d) and to compare the susceptibility of water buffalo and cattle calves to infection by the intraconjunctival route. seven of each cattle and water buffalo (bubalus bubalis) calves aged 3-6 months were inoculated intraconjunctivally with counts ranging from 1.5 × 10(7) to 1.7 × 10(10) colony forming units of b. abortus. animals were monitored over an 8-week period for clinical manifestations and sero ...201020614238
the nramp1aa genotype confers susceptibility to brucella abortus in water buffalo.the 3' untranslated region of the water buffalo nramp1 (natural resistance-associated macrophage protein 1) gene contains two alleles (nramp1a and nramp1b), as detected by the denaturing gradient gel electrophoresis (dgge) technique. the nramp1bb genotype is associated with resistance of water buffalo to the intracellular pathogen brucella abortus. this article provides evidence that the nramp1aa genotype is associated with susceptibility to the same pathogen. susceptibility or resistance of wat ...200717334658
genetic resistance to brucella abortus in the water buffalo (bubalus bubalis).brucellosis is a costly disease of water buffaloes (bubalus bubalis). latent infections and prolonged incubation of the pathogen limit the efficacy of programs based on the eradication of infected animals. we exploited genetic selection for disease resistance as an approach to the control of water buffalo brucellosis. we tested 231 water buffalo cows for the presence of anti-brucella abortus antibodies (by the agglutination and complement fixation tests) and the nramp1 genotype (by pcr-denaturin ...200616552040
simultaneous identification of antibodies to brucella abortus and staphylococcus aureus in milk samples by flow cytometry.two flow cytometric assays are described herein. the single cytometric test (sct) detects antibodies to either brucella abortus or staphylococcus aureus in the serum or milk of a cow or water buffalo. the double cytometric test (dct) detects both anti-b. abortus and anti-s. aureus antibodies concurrently. in the sct, the sample to be tested is incubated in succession with the antigen (either b. abortus or s. aureus) and the proper secondary antiserum (fluorescein isothiocyanate-labelled rabbit a ...19989508316
shedding of brucella abortus rough mutant strain rb51 in milk of water buffalo (bubalus bubalis).the objective of this study was to determine if brucella abortus rough mutant strain rb51 (srb51) is eliminated in buffalo milk. thirty brucella-free female buffaloes were used in this study: ten 4-5 years old were inoculated with the triple of the recommended calfhood dose of srb51 by subcutaneous route, ten 2-3 years old at the first lactation were previously vaccinated twice as calves with triple the recommended calf dose of rb51, while five 4-5 years old and five 2-3 years old not vaccinated ...200919362381
a diagnostic protocol to identify water buffaloes (bubalus bubalis) vaccinated with brucella abortus strain rb51 vaccine.the use of live vaccine strain rb51 for vaccination of domestic water buffaloes (bubalus bubalis) at risk of infection with brucella abortus is permitted notwithstanding the plans for the eradication and only under strict veterinary control. the antibodies induced by rb51 vaccination are not detectable using conventional diagnostic techniques; therefore, it is necessary to have a specific diagnostic tool able to discriminate vaccinated from unvaccinated animals. the combination of a complement f ...201526129660
association of microsatellite (gt)n polymorphism at 3'utr of nramp1 with the macrophage function following challenge with brucella lps in buffalo (bubalus bubalis).brucella abortus is a facultative intracellular pathogen that survives and replicates in host macrophages. hence, macrophage function plays an important role in influencing natural resistance/susceptibility to intracellular pathogen. the natural resistance associated macrophage protein 1 (nramp1; erstwhile referred as ity/lsh/bcg), a transmembrane protein, regulates activity of macrophages against intracellular pathogens. in bovine, natural resistance to brucellosis is significantly associated w ...200818078724
protective effect of the nramp1 bb genotype against brucella abortus in the water buffalo (bubalus bubalis).we tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the nramp1 genotype (by capillary electrophoresis). four alleles (nramp1a, -b, -c, and -d) were detected in the 3' untranslated region of the nramp1 gene. the bb genotype was represented among only controls, providing evidence that this genotype confers resistance to brucella abortus. the monocytes from ...200717145946
efficacy of brucella abortus vaccine strain rb51 compared to the reference vaccine brucella abortus strain 19 in water buffalo.approximately 250,000 water buffalo (bubalus bubalis) live in the campania region of southern italy where the breeding of this species is very popular. of these animals, almost 150,000 are concentrated in the caserta province where the prevalence of brucella abortus in this species represents approximately 20% at herd level. the italian brucellosis eradication programme provides a slaughter and vaccination strategy for this province. b. abortus strain rb51 (rb51) has become the official vaccine ...201020391363
evaluation of a fluorescence polarization assay for the detection of serum antibodies to brucella abortus in water buffalo (bubalus bubalis).the fluorescence polarization assay (fpa) was evaluated for the serological diagnosis of brucellosis in water buffalo (bubalus bubalis) in southern italy. this assay uses o-polysaccharide prepared from brucella abortus lipopolysaccharide conjugated with fluorescein isothiocyanate as a tracer. it has many methodological advantages over older, more established tests and can be performed in a fraction of the time. sera from 890 buffalos from the campania region - 526 positive sera and 364 negative ...200818599128
mannose-binding lectin haplotypes influence brucella abortus infection in the water buffalo (bubalus bubalis).a case-control study established that the haplotype pair hya/hya at the mbl (mannose binding lectin) locus of water buffalo is associated with resistance to brucella abortus infection (p < 10(-7)) and the haplotype pairs lyd/lyd with susceptibility to the same pathogen (p < 10(-7)). the subjects included in the present study were tested twice-at a 1-month interval-for the presence of anti-b. abortus antibodies in the serum by agglutination, complement fixation and flow cytometry. cases (335 subj ...200818330558
serologic responses, biosafety and clearance of four dosages of brucella abortus strain rb51 in 6-10 months old water buffalo (bubalus bubalis).thirty water buffalo were obtained from a brucellosis-free farm in order to evaluate antibody responses, bacterial clearance and safety to brucella abortus strain rb51 vaccine in a dose response study. the animals were randomly divided into five treatment groups. groups i-v received the recommended dose of rb51 vaccine (rd) once, rd twice 4 weeks apart, double rd once, double rd twice 4 weeks apart and saline once, respectively. antibody responses to rb51 were monitored at 2, 4, 6, 8, 10, 12, 16 ...200616112746
evaluation of cell-mediated immune responses and bacterial clearance in 6-10 months old water buffalo (bubalus bubalis) experimentally vaccinated with four dosages of commercial brucella abortus strain rb51 vaccine.thirty water buffalo, obtained from a brucellosis-free farm, were used to evaluate cell-mediated immune responses and bacterial clearance in response to vaccination with brucella abortus strain rb51 (rb51) in a dose-response study. the animals were randomly divided into five treatment groups. groups i--v received the recommended dose (rd) of rb51 vaccine once, rd twice 4 weeks apart, double rd once, double rd twice 4 weeks apart and saline once, respectively. cell-mediated immune response to rb5 ...200515963819
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