| the pathology of spontaneous paratuberculosis in the north american bison (bison bison). | gross and histopathologic examinations were performed on 70 north american bison (bison bison) from a mycobacterium avium paratuberculosis culture-positive herd. the bison examined were part of a breeding herd totaling 2,800 animals. eight of 70 (11%) animals had gross findings of intestinal mucosal thickening, and 16 of 70 (23%) of the animals had enlarged mesenteric lymph nodes. histologic lesions compatible with johne's disease were diagnosed in 30 of 70 (43%) bison on the basis of the demons ... | 2000 | 11055866 |
| the histopathologic diagnosis of subclinical johne's disease in north american bison (bison bison). | the morphologic changes of subclinical johne's disease in north american bison (bison bison) are characterized by microgranulomas composed of epithelioid macrophages and individual multinucleate giant cells of langhans'-type occasionally containing individual cytoplasmic acid-fast bacilli compatible with mycobacterium avium paratuberculosis. the microgranulomas are best visualized in the mesenteric lymph nodes of infected subclinical animals. macrophages that can be confused with infection-assoc ... | 2000 | 11118717 |
| typing of is 1311 polymorphisms confirms that bison (bison bison) with paratuberculosis in montana are infected with a strain of mycobacterium avium subsp. paratuberculosis distinct from that occurring in cattle and other domesticated livestock. | isolates of mycobacterium avium subsp. paratuberculosis from nine bison (bison bison) from montana, united states of america, were compared with those from other species from the united states of america. all 16 isolates had a novel is 1311 genotype in which all copies of the element possessed a thymidine-cytosine nucleotide variation at base position 223. isolates from bison were termed b strain. thirteen isolates from cattle and goats were polymorphic at this locus, a status previously recogni ... | 2001 | 11352594 |
| immune responses after oral inoculation of weanling bison or beef calves with a bison or cattle isolate of mycobacterium avium subsp. paratuberculosis. | paratuberculosis is endemic in domestic and wild ruminants worldwide. we designed the following study to compare host immune responses and pathologic changes in beef calves and bison calves after challenge with either a cattle or bison (bison bison) strain of mycobacterium avium subsp. paratuberculosis. in the first part of the study, six bison and six beef calves were orally inoculated with a cattle isolate of m. avium subsp. paratuberculosis over a 2 wk period. in the second part, an additiona ... | 2003 | 14567215 |
| rapid and sensitive detection of mycobacterium avium subsp. paratuberculosis in bovine milk and feces by a combination of immunomagnetic bead separation-conventional pcr and real-time pcr. | immunomagnetic bead separation coupled with bead beating and real-time pcr was found to be a very effective procedure for the isolation, separation, and detection of mycobacterium avium subsp. paratuberculosis from milk and/or fecal samples from cattle and american bison. samples were spiked with m. avium subsp. paratuberculosis organisms, which bound to immunomagnetic beads and were subsequently lysed by bead beating; then protein and cellular contaminants were removed by phenol-chloroform-isop ... | 2004 | 15004056 |
| comparison of diagnostic detection methods for mycobacterium avium subsp. paratuberculosis in north american bison. | tissues and fecal material were collected from 14 north american bison (bison bison) that were suspected of having johne's disease and analyzed for the presence of mycobacterium avium subsp. paratuberculosis (m. paratuberculosis). sections of ileum, ileal-cecal lymph node, and three sequential sections of jejunum with their associated mesenteric lymph nodes were taken from each animal. fecal culture indicated that 5 of 14 (35.7%) animals were infected, whereas cultures from tissues detected 12 o ... | 2005 | 15657271 |
| molecular typing of mycobacterium avium subspecies paratuberculosis strains from different hosts and regions. | the is1311 polymerase chain reaction-restriction endonuclease analysis was used to detect genetic differences among 38 mycobacterium avium subsp. paratuberculosis (map) isolates from cattle, sheep, goats and bison from distinct regions of spain, india and the united states of america (usa). in spain, all eight bovine isolates, three out of six caprine isolates and one of ten ovine isolates were of the c type, while the other nine ovine isolates and three caprine isolates were of the s type. in i ... | 2005 | 16642774 |
| mycobacterium avium subspecies paratuberculosis in bison (bison bison) from northern canada. | nested polymerase chain reaction (pcr) using the mycobacterium avium subspecies paratuberculosis (map)-specific region, locus 251, was used as a screening tool for the detection of map dna in fecal samples from northern canadian bison herds. further characterization of positive samples (26/835) was performed because map dna was found without signs of disease. strain typing, using pcr-restriction endonucleas assay (rea), was limited to two samples but revealed that the samples corresponded to a c ... | 2007 | 17984279 |
| serological studies to determine the occurrence of johne's disease and mycoplasma infection in the northern-east polish population of european bison (bison bonasus). | a serological study of twenty three european bison (bison bonasus) derived from northern-east poland for the seroprevalence of mycobacterium avium subsp. paratuberculosis, mycoplasma bovis, mycoplasma mycoides subsp. mycoides sc, mycoplasma agalactiae and mycoplasma capricolum subsp. capripneumoniae was conducted. only specific antibodies to m. bovis were detected in two animals (8.7%) which were connected with the clinical signs and macroscopic anatomopathological lesions. | 2014 | 25638988 |
| contrasting results of culture-dependent and molecular analyses of mycobacterium avium subsp. paratuberculosis from wood bison. | reduced to near extinction in the late 1800s, a number of wood bison populations (bison bison athabascae) have been re-established through reintroduction initiatives. although an invaluable tool for conservation, translocation of animals can spread infectious agents to new areas or expose animals to pathogens in their new environment. mycobacterium avium subsp. paratuberculosis, a bacterium that causes chronic enteritis in ruminants, is among the pathogens of potential concern for wood bison man ... | 2013 | 23686265 |
| the modification and evaluation of an elisa test for the surveillance of mycobacterium avium subsp. paratuberculosis infection in wild ruminants. | enzyme-linked immunosorbent assay (elisa) is often used to test wildlife samples for mycobacterium avium subsp. paratuberculosis (map) infection. however, commercially available kits are only validated for use with domestic ruminant species. a literature review was performed to document the current use of map serum elisa in wild and semi-domestic ruminants. we then modified and evaluated a commercial elisa kit (idexx mycobacterium paratuberculosis antibody test kit) for use with species for whic ... | 2013 | 23302439 |