| antimicrobial susceptibility of 1042 strains of streptococcus mutans and streptococcus sobrinus: comparison from 1985 to 1989. | a total of 1042 strains of streptococcus mutans and streptococcus sobrinus isolated between 1985 and 1989 were tested to study the evolution of their sensitivity to penicillin, amoxycillin, amoxycillin/clavulanic acid, cefuroxime, tetracycline, erythromycin, spiramycin, acetyl spiramycin, lincomycin and clindamycin. the strains were taken from stock cultures and isolated from human saliva and dental plaque. the minimal inhibitory concentration (mic) was determined by an agar dilution method. exc ... | 1991 | 1945497 |
| construction of noti restriction map of the streptococcus mutans genome. | streptococcus mutans and streptococcus sobrinus are the major causative organisms of human dental caries. pulsed-field gel electrophoresis (pfg) showed that the restriction enzyme noti produced ten and six dna fragments from the genomes of s. mutans strain mt8148 and s. sobrinus strain 6715, respectively. the sizes of the chromosomes of s. mutans and s. sobrinus were each estimated to be about 2200 kb. the noti restriction map of s. mutans mt8148 genome was constructed by southern blot analysis ... | 1990 | 2079621 |
| binding of streptococci of the "mutans" group to type 1 collagen associated with apatitic surfaces. | the present study surveyed the ability of 21 strains of cariogenic streptococci of the "mutans" group to bind to human type 1 collagen adsorbed on hydroxyapatite (ha) surfaces. all strains of streptococcus cricetus (serotype a) and streptococcus rattus (serotype b) tested attached strongly to collagen-treated ha (c-ha). streptococcus mutans strains of serotype c and streptococcus sobrinus strains of serotype d or g attached poorly; or not at all, to c-ha. s. mutans strains omz-175 (serotype f) a ... | 1990 | 2080067 |
| [demonstration of streptococcus sobrinus in human oral cavity]. | from 133 saliva samples of humans we isolated 32 strains (24%) of streptococcus sobrinus on an bycsb selective medium. the very hard colonies of s. sobrinus on this medium were characterized by a distinct chalky white halo of water insoluble glucan ("glucan-halo") produced in large amounts from the sucrose by extracellular glucosyltransferase which has diffused out into the isolation medium. all of the isolated s. sobrinus strains fermented ordinarily mannitol and sorbitol, but not raffinose and ... | 1990 | 2206607 |
| prevalence of streptococcus sobrinus in relation to dental caries in children from iceland and the netherlands. | the aim of this study was to investigate the presence of s. sobrinus and s. mutans in specimens of dental plaque and saliva of children five years of age in reykjavik, iceland (study 1) and in samples of dental plaque from children nine years of age in amsterdam, the netherlands (study 2). the immuneblotting technique (ibt) was a suitable method to evaluate the presence and numbers of s. mutans and s. sobrinus in human dental plaque and saliva. in study 1, eighty-four children were evaluated bac ... | 1990 | 2212191 |
| competitive displacement of mutans streptococci and inhibition of tooth decay by streptococcus salivarius tove-r. | the ability of streptococcus salivarius tove-r to displace virulent representatives of the most prevalent human mutans streptococci from the teeth of rats, and thereby to inhibit caries, was studied. streptococcus mutans 10449s- or streptococcus sobrinus 6715-13wt-infected specific-pathogen-free rats consuming a high-sucrose diet were inoculated by tove-r. the infectants were differentially recovered from swabs of the teeth over the time course of infection and from sonically treated material of ... | 1985 | 3980093 |
| in vitro modulation of oral bacterial adhesion to saliva-coated hydroxyapatite beads by milk casein derivatives. | bovine caseinate, derivatives of its glycosylated moiety [caseinoglycomacropeptide (cgp)], and caseinophosphopeptides were evaluated as inhibitors of adhesion of oral bacteria to saliva-coated hydroxyapatite beads (s-ha). all milk casein-derived components behaved as potent inhibitors of streptococcus sanguis omz 9 and streptococcus sobrinus omz 176 adhesion to s-ha, whereas neither bovine serum albumin nor polyethyleneglycol were able to interfere with the adhesion of these strains. by contrast ... | 1994 | 7478758 |
| characterization of glucosyltransferaseb, gtfc, and gtfd in solution and on the surface of hydroxyapatite. | glucosyltransferaseb, gtfc, and gtfd were purified by hydroxyapatite column chromatography, followed by ultrafiltration from the culture supernatant fluids of three streptococcus milleri constructs (gift from dr. h.k. kuramitsu) which harbored individual gtf genes of streptococcus mutans gs5. gtfb, gtfc, and gtfd were enzymatically active both in solution and in an experimental pellicle (ha-cws-gtf) formed by adsorbing gtf onto the surface of clarified human whole saliva (cws)-coated hydroxyapat ... | 1995 | 7499593 |
| immunoglobulin a antibodies to mutans streptococci in human saliva and serum comparing fresh and subcultivated strains and activity in repeated saliva samples. | the aims of this study were i) to characterize and compare the sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein patterns of reference streptococcus mutans and streptococcus sobrinus strains, subcultivated for years, with fresh isolates of the same serotype; ii) to study possible differences between the human salivary immunoglobulin a (iga) activity to reference strains and to fresh bacterial isolates of the saliva donors; iii) to examine for potential differences in the salivary ... | 1994 | 7808769 |
| acid production by human strains of streptococcus mutans and streptococcus sobrinus. | acid production by washed suspensions of human strains of streptococcus mutans (n = 18) and streptococcus sobrinus (n = 12) was measured. the strains were isolated from infants and adolescents with varying caries experience. some of these strains and two laboratory strains (1 s. mutans and 1 s. sobrinus) had been tested in an earlier study for their cariogenicity in hamsters. further, 3 streptococcus sanguis strains and 1 s. sobrinus laboratory strain were included. acid production was determine ... | 1995 | 8521443 |
| effects of bovine immune and non-immune whey preparations on the composition and ph response of human dental plaque. | colostral products from non-immunized cows (cp) and cows immunized with mutans streptococci (ip) were used as mouth rinses in a short-term human study. the acidogenic potential of the products was tested and found to be negligible in vivo before application to subsequent rinsing tests. at first, all the participants received a professional tooth cleaning, after which they rinsed with one of the solutions (ip; cp; water) three times per day for 3 d. after each rinsing period, the resting ph and d ... | 1999 | 10467939 |
| colostral proteins from cows immunised with streptococcus mutans/s. sobrinus support the phagocytosis and killing of mutans streptococci by human leucocytes. | passive immunisation, based on bovine colostral preparations, is an area of active research. specific bovine antibodies inhibit the virulence factors of target pathogens but the interactions between whey preparations and human immune defence cells are not well known. bovine colostrum inhibits the phagocytic activity of bovine leucocytes and this may reflect the biological activity of immunoglobulins in it. therefore, this study aimed to examine the effects of bovine whey protein preparations fro ... | 1999 | 10510968 |
| current understanding of the cause of dental caries. | dental caries-associated oral streptococci are called the mutans streptococci, with streptococcus mutans and streptococcus sobrinus being the most prevalent caries-associated organisms in humans. strains of the mutans streptococci are highly conserved within not only mothers and their children but also racial groups, suggesting vertical transmission of this organism within human populations. it has been found that the mother-child infection route of the mutans streptococci can be prevented by si ... | 2000 | 10777849 |
| the anticariogenic effect of amine fluorides on streptococcus sobrinus and glucosyltransferase in biofilms. | dental caries is a chronic infectious disease caused by the accumulation of bacterial plaque (biofilm) on tooth surfaces. antibacterial agents, in addition to other preventive measures, can control dental plaque accumulation. amine fluorides (amf) are known anticaries agents for over 30 years. the purpose of our study was to assess the adsorption and desorption of amf to experimental dental biofilm and to evaluate the effect of amf on streptococcus sobrinus 6715 and glucosyltransferase (gtf) act ... | 2000 | 10867426 |
| antimicrobial activity of silver nitrate against periodontal pathogens. | metal ions were evaluated as potential antimicrobial agents suitable for local delivery in the oral cavity for the treatment of periodontitis. silver nitrate, copper chloride, and zinc chloride were tested for antimicrobial activity in in vitro killing assays conducted in phosphate buffered saline with a series of oral bacteria including gram-negative periodontal pathogens and gram-positive streptococci. copper and zinc salts failed to exhibit strong and consistent activity against periodontal p ... | 2001 | 11327077 |
| induction and 3d reconstruction of caries-like lesions in an experimental dental plaque biofilm model. | the aim of this study was to examine an in vitro dental plaque model for volumetric assessment and 3d reconstruction of experimentally bacterial induced caries-like lesions. twenty-three extracted, impacted human molars were coated with a varnish leaving a 3 x 3 mm window on the smooth surface. the teeth were coated with human saliva and streptococcus sobrinus 6715 adhered onto the enamel via a sucrose dependent mechanism. the coated teeth were incubated for 1, 7, 14 and 28 days. at the end of e ... | 2001 | 11556956 |
| adhesion in vitro of oral streptococci to porcelain, composite resin cement and human enamel. | we investigated the initial adhesion in vitro of oral streptococci to porcelain inlays. four strains of streptococci, streptococcus mitis nctc12261, streptococcus oralis atcc9811, streptococcus sanguis atcc10556 and streptococcus sobrinus omz176, were used in this study. the disc specimens were made of porcelain, composite resin cement and human enamel. these specimens, with or without a saliva-coat, were immersed in a suspension of each streptococci strain at 37 degrees c for 1 hr, and the numb ... | 2000 | 11816366 |
| [anti-caries vaccines]. | even though the reduction of caries-incidence in developed countries, its increasing has been observed nowadays. the use of a vaccine was object of many researches, going under modifications and evaluations during years. wallace and mccollum showed the chance to induce experimental cavities, while clarke and mcintosh were the first underlining the roll of s. mutans and lactobacilli as efforts of the pathology. williams was the first working with humans and zinner and fitzgerald continued. so sin ... | 2002 | 12147978 |
| [rapid detection of streptococcus mutans and streptococcus sobrinus in human saliva by nested polymerase chain reaction]. | to establish a simple and rapid method to detect streptococcus mutans and streptococcus sobrinus simultaneously in human saliva. | 2003 | 12887805 |
| activity of human beta-defensin 3 alone or combined with other antimicrobial agents against oral bacteria. | the in vitro activities of human beta-defensin 3 (hbd-3) alone or combined with lysozyme, metronidazole, amoxicillin, and chlorhexidine were investigated with the oral bacteria streptococcus mutans, streptococcus sanguinis, streptococcus sobrinus, lactobacillus acidophilus, actinobacillus actinomycetemcomitans, and porphyromonas gingivalis. hbd-3 showed bactericidal activity against all of the bacterial species tested. the bactericidal effect was enhanced when the peptide was used in combination ... | 2003 | 14506056 |
| molecular interactions of surface protein peptides of streptococcus gordonii with human salivary components. | oral streptococci play a large role in dental biofilm formation, and several types interact as early colonizers with the enamel salivary pellicle to form the primary biofilm, as well as to incorporate other bacteria on tooth surfaces. interactions of surface molecules of individual streptococci with the salivary pellicle on the tooth surface have an influence on the etiological properties of an oral biofilm. to elucidate the molecular interactions of streptococci with salivary components, bindin ... | 2004 | 15271944 |
| time of initial acquisition of mutans streptococci by human infants. | the aim of this study was to detect and monitor the acquisition of mutans streptococci (ms) in healthy brazilian children. samples of 4 different sites (saliva, tongue dorsum, dental ridges, and dental plaque, if teeth were present) were collected from 33 edentulous nursery school infants (5.9+/-1.5 month-old), using sterilized swabs, bi-monthly for 24 months. saliva samples from the mothers were collected only once. after inoculation, and incubation typical morphotype colonies, were isolated an ... | 2004 | 15366617 |
| inflammatory immune responses by water-insoluble alpha-glucans. | water-insoluble alpha-glucans are synthesized from sucrose by glucosyltransferase-i of mutans streptococci and play an important role in the development of dental plaque. several types of beta-glucans in fungal cell wall components and water-soluble alpha-glucans from streptococcus mutans are known to modulate innate immunity. in the present study, we investigated whether water-insoluble alpha-glucans also induced inflammatory innate immune responses. our results showed that water-insoluble alph ... | 2007 | 17314256 |
| targeted profiling of oral bacteria in human saliva and in vitro biofilms with quantitative real-time pcr. | an in vitro plaque model based on the use of human salivary bacteria and tooth-like surfaces was previously developed for studying the formation of oral biofilm and its use for pre-clinical testing of candidate antimicrobial or antiplaque agents. in this study, a quantitative taqman pcr assay (qpcr) was developed to compare the bacterial compositions of in vitro biofilms to parent saliva samples, and to determine the relative contributions of different species in the formation of the oral biofil ... | 2007 | 17653931 |
| differentiation of banding patterns between streptococcus mutans and streptococcus sobrinus isolates in rep-pcr using eric primer. | streptococcus mutans and streptococcus sobrinus are considered to be important bacterial species in the initiation of human dental caries. therefore, the establishment of a reliable genotyping method to distinguish s. mutans from s. sobrinus is of central importance. | 2011 | 22145074 |