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[fractionation of the trichinella spiralis antigen and allergy diagnosis trails].experiments were carried out to fractionate an antigen of trichinella spiralis with sephadex g-50 and g-100. the specificty of the fractions has been followed up by means of the intradermal allergic reaction in six pigs, experimentally infected with trichenalla spiralis, one with trichocephalus suis and three, born by trichinellosis-affected sows. the second fraction has been tested also on four pigs, experimentally inoculated with ascaris suum, nine--with ascarsis suum and oesophagostomum and 1 ...197888790
intestine of ascaris: oxygen consumption, fermentation acids, and anaerobic synthesis of protein.the oxygen uptakes by pieces of gut from the porcine roundworm, ascaris suum, under po2 10 to 710 torr were from 0.6 to 3.0 mul/mg dry wt/hr (qo2). increasing concentrations of tissue, 30 to 90 mg (dry wt/3 ml) decreased qo2 in air from 1.9 to 1.1 and only technical grade catalase was found to relieve this inhibition. conversely aerobic fermentation, judged by acid production, was directly proportional to the amount of tissue present: the acids from this fermentation were 2-methyl-butyric (1% of ...1975241780
esophagogastric ulcers in swine infected with ascaris suum. 1979473495
chromosome diminution in ascaris suum. two-fold increase of nucleosomal histone to dna ratios during development.the swine intestinal nematode, ascaris suum, eliminates chromatin material from its primordial somatic cells during early embryogenesis. a technique for isolation of nuclei from pre- and post-diminution stage embryos has been developed and these isolated nuclei were used in investigations of nuclear events during diminution. the amount of dna per nucleus determined by diphenylamine assays and isotope dilutions was 0.66 pg and 0.29 pg in pre- and post-diminution nuclei, respectively. thus, a. suu ...1979518883
ige and igg1 antibody production by a soluble product of ascaris suum in the guinea-pig.third-stage larvae of ascaris suum cultured to the fourth stage in a chemically defined culture medium produced a substance, the 'acf antigen', which was allergenic in the guinea-pig. when three different concentrations (3.1, 31 and 62 micrograms) of the acf antigen were given intraperitoneally, only the highest concentration induced a primary ige specific antibody response (1:100 titre) as determined with the passive cutaneous anaphylaxis reaction. upon secondary exposure all concentrations dem ...1979521052
the influence of environmental temperature on the rate of development of ascaris suum eggs in great britain.the rate of development of ascaris suum eggs was recorded during 18 months in england. every second or third week unembryonated eggs were placed in an unheated animal house and their development was observed at weekly intervals. eggs underwent no development during november to may. embryonation was most rapid during june, july and august. the shortest time for eggs to reach the infective stage was two weeks in 1976 and four weeks in 1977. a relationship was apparent between the rate of developme ...1979523803
ascaris suum: hatching of embryonated eggs in swine. 1977560316
parasites of domestic and wild animals in south africa. viii. helminths in pigs kept under semi-intensive conditions.the seasonal incidence of nematode infestations in pigs raised under semi-intensive conditions was determined by the monthly slaughter of 2--4 tracer pigs exposed to infestation in an earthernfloored pen for periods of 1 or 2 months. although worm burdens were generally small, ascarops strongylina appeared to be more prevalent from november to march than during the other months of the year. once ascaris suum became established in the pen nearly all the pigs became infested. the number of worms n ...1978568231
[parasites in the gdr.1. studies on helminths occurrence in european wild pigs (sus scrofa)].in two different hunting-grounds in the north-eastern part of the gdr from november 1972 to march 1974 the authors tested a series of 102 killed wild boars, aged mostly from 7 month to 3 years by means of helminthological dissection and by coprological methods for helminthic stages. from more than 30 well known species of helminths the following 10 species were found: cysticercus tenuicollis, echinococcus hydatidosus, ascaris suum, ascarops strongylina, physocephalus sexalatus, globocephalus uro ...1978646175
proteolytic enzymes and enzyme inhibitors in ascaris suum. i. proteolytic enzymes and enzyme inhibitors in the gut. 1977754545
proteolytic enzymes and enzyme inhibitors in ascaris suum. iii. in vitro study of proteinase inactivation by the parasite. 1977754548
[clinical examination of the blood of wild boars (sus scrofa l.) naturally infested with parasites following administration of anthelminthics].with 24 head of wild boars (sus scrofa l.) of both sexes of the age of one to two years, kept in a game preserve and naturally infected with parasites of the species mestastrongylus pudendotectus, m. elongatus, ascarops strongylina, physocephalus sexalatus, ascaris suum, globocephalus urosubulatus, oesophagostomum dentatum, trichocephalus suis, eimeria debliecki, and e. perminuta, three experiments were performed with feeds premedicated with anthelmintics: pyrantel tartrate + diethylcarbamazine ...1976820035
infection of weanling pigs with known numbers of ascaris suum fourth stage larvae.nineteen weanling pigs were each infected per os with 200 fourth stage ascaris serum larvae and were fed either a low protein or a high protein diet for 8 weeks. worms expelled during the 8 weeks were recovered and all worms remaining in the gastrointestinal tract at slaughter were collected. fifty-four percent of all worms were recovered at slaughter. ten of the 19 animals maintained an infection for 8 weeks; the other 9 showed no signs of a. suum infection. a total of 377 worms were recovered. ...1977832477
an evaluation of ethyl-6-ethoxybenzothiazole-2-carbamate (sch 18099) for anthelmintic activity in domestic animals.ethyl-6-ethoxybenzothiazole-2-carbamate (sch 18099) was evaluated for efficacy against natural helminth infections in ponies, pigs, lambs and chickens. sixteen critical trials were conducted in ponies at dosages of 15 to 150 mg/kg. at 15 mg/kg, efficacy against adult and larval oxyuris equi was 100% and 91% and against small strongyles it was 98%. efficacy levels were 95% against strongylus vulgaris and s. edentatus at the 20 mg/kg dosage. in two trials at 100 mg/kg efficacy against parascaris e ...1977864223
anatomy of the intestine of ascaris suum: a three dimensional study with silicone rubber casts. 1977884638
[the influence of ascaris suum infection upon growth rates in pigs (author's tranls)].the influence of experimental ascaris suum infection upon growth rates in pigs has been studied in two experiments with disease-free pigs. in pigs where a patent ascaris infection was established growth rates were decreased by appr. 20% compared with parasite-free controls. the experiments have revealed that the negative influence of ascaris suum upon growth is caused both by the larvae during migration through the lungs and by the presence of adult worms in the intestine.1976940739
differential responses of duroc, hampshire, and crossbred pigs to a superimposed experimental infection with the intestinal threadworm, strongyloides ransomi.in both spring and fall, 12 duroc, 12 hampshire, and 12 duroc times hampshire f1 weanling pigs all reared under the same management were fed in pens of 3 to slaughter weights. three duroc and 4 hampshire boars, essentially unrelated within breed, were used in sampling the breeds. swine herd management allowed pig infection with strongyloides ransomi and ascaris suum, but neither clinical nor subclinical parasitism was evident in the herd. pigs were percutaneously exposed by pens within breed and ...19751138042
[the destruction of parasitic resistant stages in sludge by irradiation with low accelerating voltage electrons (author's transl)].the destroying effect of ionizing radiation on parasitic resistant stages in sludge has been tested. suitable for that process is an electron beam accelerator which will be provided with energy from the electric power supply network which can be switched on and off according to the requirements. such modern utilities have an enormous beam capacity and a high operating safety. the process is working according to the continuous flow principle and at room temperature. in a series of 13 experiments ...19751189798
scanning electron microscopy of the lip denticiles of ascaris suum adults of known ages.purebred hampshire pigs, farrowed and maintained under conditions precluding extraneous helminth infection, were exposed to a single dose of 10,000 ascaris suum infective eggs. the pigs were killed at intervals of 28, 41, 55, 86, 115, 145, 175, and 206 days after infection. at necropsy, no gross lesions were found in the lungs or livers of infected pigs. the worms were recovered from the small intestine, identified, counted, and fixed. the heads were excised, critical point dried, mounted en fac ...19761263037
cuticular reactivity of the early larval stages of ascaris suum: adhesion and degranulation of polymorphonuclear leukocytes of the surface of opsonized larvae of a. suum. 19761279081
isoenzymatic pattern and structure of glutamate dehydrogenase from ascaris suum.the isoenzymatic pattern of glutamate dehydrogenase (gdh) has been described for ascaris suum a parasite of sus scrofa domestica. only one band of activity has been revealed, suggesting a monomorphic condition for this enzyme. also, the structure of gdh has been assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and silver staining. only one subunit was present with a molecular weight of about 55,000. a hexameric structure for gdh of a. suum is suggested.19921293199
extracellular recordings from the motor nervous system of the nematode, ascaris suum.1. the close association of muscle and neurons in ascaris suum makes it difficult to determine whether spikes recorded from nerve cords originate in muscle or neurons. we have developed criteria that distinguish muscle and neuronal activity. there are two categories of extracellular spikes. 2. the first category consists of spikes with a wide range of amplitudes, marked by large spikes. these spikes, which can be recorded over lateral muscle and over the dorsal and ventral nerve cords, are aboli ...19921328624
patent infections of ascaris suum in pigs: effect of previous exposure to multiple, high doses of eggs and various treatment regimes.fifty-four crossbred, 4-week-old pigs divided into nine equal groups were used to test whether multiple inoculations with high numbers of a. suum eggs with or without anthelmintic would result in patent infections. all pigs exposed to multiple prechallenge inoculations of 500, 1000, 2000, 5000, 10,000 and 20,000 and challenged orally 2 weeks later with 10,000 eggs harboured adult worms. when prechallenge infections were removed by pyrantel tartrate treatment the animals were more susceptible to ...19921399243
interaction between ascaris suum and pasteurella multocida in the lungs of mice.in an experiment including 8 groups of 15 mice, the effect of migrating ascaris suum larvae in the lungs on the establishment and pathogenicity of aerosol exposure to pasteurella multocida was investigated. following aerosol exposure to p. multocida, mice with migrating a. suum in their lungs developed more severe pneumonia and septicaemia than did parasite-free mice. the parasite-induced effect on bacterial pathogenicity was more marked for a non-toxin-producing p. multocida as compared with a ...19921438140
relationship of airway responsiveness to agents causing bronchoconstriction and cough in sensitized guinea-pigs.the relationship between airway responsiveness to bronchoconstrictor- and cough-inducing stimuli has been examined in ascaris suum-sensitized conscious guinea-pigs. guinea-pigs were sensitized to ascaris suum [4000 pnu and 100 mg al(oh)3 i.p. on days 1 and 7] and then challenged with aerosolized antigen on days 21, 28 and 35. at day 35, antigen-exposure produced an early bronchoconstrictor response (ebr) and in about 50% of the animals also a late bronchoconstrictor response (lbr) commencing 4-8 ...19921446141
ascaris suum--vaccination of mice with liposome encapsulated antigen.vaccination with liposome encapsulated adult crude antigen with and without coencapsulated immunomodulator (levamisole) in a mice/larval ascaris suum model provided protection against a challenge infection (2000 eggs) in mice immunised by immobilised antigen. the best results (88.9% protection) were obtained with a combination of two doses of liposome entrapped antigen with leamisole. vaccination with liposome vaccine without modulator was slightly less effective (78.7% protection). a single dos ...19921496793
resistance to ascaris suum in parasite naïve and naturally exposed growers, finishers and sows.commercially reared growers, finishers, and sows of danish landrace x yorkshire crossbred were inoculated orally with ascaris suum at 50 eggs kg-1 body weight. white spots on the serosal surface of livers and total larval recoveries from lungs were recorded 7 days later. the response in pigs originating from a specific pathogen free and parasite free herd (parasite naïve) was observed in the three different age groups and compared with age-matched pigs from a herd maintained in a facility contam ...19921532874
response to repeated inoculations with ascaris suum eggs in pigs during the fattening period. i. studies on worm population kinetics.this experimental study on pigs was designed to simulate natural, long-term exposure to ascaris suum under modern management conditions. parasite kinetics were followed in pigs receiving a. suum eggs as repeated trickle inoculations at two dose levels beginning at a body weight of 25 kg until their slaughter at 90 kg (baconers). in pigs inoculated twice weekly with 500 eggs, there was an initial marked rise in the numbers of hepatic milk spots, but as early as around week 6 after the start of in ...19921534170
effect of glucan preparation on immunocompetent cells and phagocytic ability of blood leucocytes in experimental ascariosis of pigs.in experimental ascariosis (ascaris suum) of pigs a preparation of glucan combined with pig immunoglobulin and zinc was found to significantly elevate the levels of both t and b lymphocytes in the blood, and to significantly stimulate the phagocytic ability of monocytes and neutrophilic granulocytes in experimental pigs. the protective effect of the preparation, assessed by the reduction in the number of migrating ascarid larvae in the lungs of pigs was 65%.19921561757
efficacy of an in-feed preparation of ivermectin against endoparasites and scabies mites in swine.in 2 trials, the efficacy of an in-feed preparation of ivermectin was evaluated in 40 pigs naturally infected with endoparasites and sarcoptes scabiei var suis. treated pigs (n = 10 in each trial) were fed a ration containing 2 ppm ivermectin for 7 days, followed by consumption of a nonmedicated ration for the remainder of the trial. control pigs (n = 10 in each trial) were fed a complete, nonmedicated ration for the duration of the trial. pigs in trial a were monitored for 14 days after treatme ...19921586019
[ascaris suum infection as a cause of clinical problems in cattle?].a sudden decrease in milk yield, increased respiratory rate and occasional coughing were observed in dairy cows on two farms in spring 1991. pigs were also kept on these farms, and pastures grazed by the cattle had been fertilised with pig slurry. laboratory investigations of some of the cattle showed eosinophilia and high elisa titres of antibodies against ascaris suum. on one farm the clinical symptoms disappeared after the animals had been treated with oxfendazole and on the other farm the sy ...19921604447
ascaris suum: are trypsin inhibitors involved in species specificity of ascarid nematodes?inhibitors of porcine trypsin were prepared from aqueous extracts of the parasitic nematodes ascaris suum (hogs) and ascaris lumbricoides (human). in this study three experiments were performed. (1) polyclonal antibodies were prepared against one isoform of trypsin inhibitor from each parasitic nematode. each antibody reacted with all isoforms from itself as well as all isoforms from the other parasite. (2) association equilibrium constants were measured by titrating host trypsins (porcine or hu ...19921639157
identification of tubulin isoforms in different tissues of ascaris suum using anti-tubulin monoclonal antibodies.three monoclonal antibodies specific to alpha- and beta-tubulin were used to examine the expression of tubulin isoforms in the intestine, reproductive tract and body wall muscle of a. suum. the tubulins were found to be different in their isoelectric points, number of isoforms and peptide maps with western blot analysis of one-dimensional polyacrylamide gel confirming the presence of alpha-, beta 1- and beta 2-tubulin. commercial cross-reactive anti-alpha and anti-beta mabs 356 and 357 recognize ...19911787032
[helminth infections in wild boars kept in enclosures in southern germany: severity of infections and fecal intensity].to determine the intensity of helminth infection and egg output with regard to age and sex of wild boar, stomachs and intestines of 124, lungs of 62, and livers of 39 animals from 5 enclosures in southern germany were examined. the evaluation of the intensity of infection showed 579.4 worms per animal for hyostrongylus rubidus, 510.4 for globocephalus longemucronatus, 476.9 for oesophagostomum dentatum, 476.9 for o. quadrispinulatum, 254.2 for metastrongylus pudendotectus, 176.9 for m. salmi, 14 ...19911796467
transmission of intestinal helminths in danish sow herds.faecal samples from sows and their litters, all untreated with anthelmintics, were examined in eight sow herds, ranging from very intensive to very traditional management. four helminth genera were recorded, namely oesophagostomum sp., ascaris suum, trichuris suis and strongyloides ransomi. the herds with the most intensive management were only infected with a. suum, while the more traditionally managed herds were infected with three or four species. in one herd, the sows showed a small relative ...19911832796
[dissemination and survival of endoparasite embryos in the environment].pig farms with the annual capacity from five to 20 thousand head represent one of the crucial fields of agriculture influencing the environment in the csfr. daily production of large amounts of liquid excrements (150-300 m3), as a result of high concentrations of animals, poses not only technical but also sanitary problems closely related to the animal and man's health. in a long-term (267 days) field study the authors followed the development and survival of eggs of the model helminth ascaris s ...19911841477
[livestock manure as a vector for parasites--a report of experiences].analysing the results of own investigations and informations in literature a review is given about the role of manure specially in stables of high-intensity cattle and pig production as a vector for exogenic parasite stages. in the course of investigations--in most cases simulating practice conditions--so-called indicator germs were used as test material. in cattle stables manure is significant as a vector for eimeria species, cryptosporidium parvum, sarcocystis species, taenia saginata and fasc ...19911914947
ascaris suum: stage-specific differences in lectin binding to the larval cuticle.a glycosylated component with affinity for wheat germ agglutinin (specific binding to n-acetyl-d-glucosamine monomers and oligomers) and weak affinity for poke weed mitogen (specific binding to n-acetyl-d-glucosamine oligomers) was detected temporally on the surface of ascaris suum larvae developing in vitro and on in vivo-derived larvae. the component was identified on the surface of in vitro-derived late second stage larvae, on all late third stage larvae (derived from pig lung), and all fourt ...19911915752
performance of pigs with mixed nematode infections before and after ivermectin treatment.ninety crossbred parasitized pigs were used in two controlled experiments to compare performance before and after ivermectin treatment. four groups of 15 pigs were sequentially infected with 2000 ascaris suum, 10,000 oesophagostomum spp. and 10,000 strongyloides ransomi beginning at average pig weights of either 38 kg (experiment i) or 14 kg (experiment ii). two infected groups of pigs were treated with ivermectin on day 34 (experiment i) or 37 (experiment ii) of the experiments and all pigs wer ...19911957485
a survey of gastrointestinal parasites in pigs of the plateau and rivers states, nigeria.faecal samples were collected from a total of 1,000 pigs from the port harcourt and jos areas of the rivers and plateau states, respectively, between january 1987 and march 1988. in the jos area the parasite incidence was: ascaris suum 53.1%, trichuris suis 8.5%, hyostrongylus rubidus 13.1%, metastrongylus salmi 3.7%, strongyloides ransomi 87.7%, oesophagostomum dentatum 35.1% and eimeria spp. 2.4% while in port harcourt the incidence rate was ascaris suum 10.4%, trichuris suis 47.2%, oesophagos ...19902092354
embryonation and infectivity of ascaris suum eggs. a comparison of eggs collected from worm uteri with eggs isolated from pig faeces.ascaris suum eggs were collected from pig faeces or dissected from worms obtained from the same pigs. eggs from the two sources were allowed to embryonate in 0.1 n h2so4, in 1% buffered formalin or in tap water. the embryonation of the sulphuric acid and water cultures occurred at the same speed, while the formalin cultures developed slightly more slowly. by experimental inoculation of helminth-free pigs and subsequent counting of white spots in the livers and larvae in the lungs day 7 p.i., the ...19902099616
relationship of the prevalence of swine helminths to management practices and anthelmintic treatment in danish sow herds.during 1982-1984, the occurrence of helminths in 66 sow herds in denmark was examined by means of faecal samples. the correlations between the prevalences of ascaris suum and oesophagostomum spp. and management practices were analysed for fatteners and sows, respectively, resulting in four multivariate models. the helminth prevalences were in general low in large herds, specific pathogen-free (spf) herds and herds with low weaning age. the multivariate analyses showed that only the two latter co ...19902144677
serum levels of gastrin, insulin and glucagon as possible factors of anorexia in pigs infected once with ascaris suum.in order to determine possible mediators for development of anorexia in pigs infected with ascaris suum, serum levels of gastrin, insulin and glucagon were measured. after a single high oral dose of 100,000-200,000 embryonated eggs the serum levels of gastrin and insulin in the infected pigs did not significantly differ from those in controls. serum glucagon levels in the infected groups, however, were lower than those in controls and the difference was more evident 24 days postinoculation and l ...19902205042
effects of multiple dose infections with ascaris suum on blood gastrointestinal hormone levels in pigs.ten consecutive daily doses of infective ascaris suum eggs were administered to pigs in two experiments and the levels of gastrointestinal hormones in their blood were measured. the piglets in each experiment were divided into low-dose (ldi) and high-dose (hdi) infections and control groups. infected pigs had lower feed consumption, lower weight gains, and lower feed efficiency than control pigs. serum gastrin levels in infected pigs were significantly lower than the controls from days 7 to 17 p ...19902238428
[helminth infections in wild boars in enclosures in southern germany: species spectrum and infection frequency].to determine the helminth fauna of wild boars, stomachs and intestines of 124, lungs of 62, and livers of 39 animals from 5 enclosures in southern germany were examined. the evaluation of the species composition revealed at least 13 species: metastrongylus apri, m. salmi, m. pudendotectus, physocephalus sexalatus, hyostrongylus rubidus, globocephalus longemucronatus, ascaris suum, trichuris suis, oesophagostomum dentatum, o. quadrispinulatum, fasciola hepatica, c. tenuicollis and capillaria. the ...19902264058
molecular cloning and expression of an immunodominant 53-kda excretory-secretory antigen from trichinella spiralis muscle larvae.a trichinella spiralis cdna expression library was constructed in lambda gt11 from muscle larvae mrna and immunologically screened to identify genes encoding previously described immunodiagnostic excretory-secretory (es) antigens. screening the library with t. spiralis infection serum from swine or rabbit antiserum to t. spiralis es antigen identified one clone, designated tsa-12, that contains a cdna transcript 539 bp in length and codes for an apparent 123-kda beta-galactosidase fusion protein ...19902270099
capsaicin and histamine antagonist-sensitive mechanisms in the immediate allergic reaction of pig airways.the airway vascular and bronchial responses were studied in pigs sensitized with ascaris suum. ascaris, histamine (h) and capsaicin aerosol all induced a clear-cut increase in blood flow in the nasal, laryngeal and bronchial circulation with a decrease in vascular resistance of 20-40%. when delivered to the lung both ascaris and histamine, but not capsaicin, caused pulmonary airflow obstruction with increase in resistance and a fall in dynamic compliance of 40-70%. after pretreatment of pigs wit ...19902309568
evaluation of pyrantel-tartrate abbreviated ascaris suum infections for the development of resistance in young pigs against migrating larvae.crossbred young pigs were used to test whether abbreviated infections with eggs of ascaris suum can stimulate the acquisition of resistance to challenge. weanling pigs from an ascaris-free colony were kept free of a. suum until they were divided into groups at the age of 7-8 weeks. the experimental animals received pyrantel tartrate during the period when they were being exposed to increasing numbers of infective a. suum eggs and challenged 10 days after the last infective dose. liver milk-spot ...19902312230
n-terminal amino acid sequence identity between a major allergen of ascaris lumbricoides and ascaris suum, and mhc-restricted ige responses to it.a protein allergen of the parasitic nematode ascaris has been purified to homogeneity by immunoaffinity chromatography. it is the most abundant protein species in the parasite's body fluid and has been named aba-1. the allergen's molecular weight (mw) has been previously estimated at 14,000, but this sizing is currently under re-evaluation. the immunological activity of the protein was intact after purification, as attested by immunoprecipitation and passive cutaneous anaphylaxis. the ige respon ...19902335378
acquired resistance to migrating larvae of ascaris suum in young pigs by repeated drug-abbreviated infections.cross-bred 3- and 8-wk-old pigs were used to test whether drug-abbreviated infections with ascaris suum can stimulate acquired resistance to challenge. during the immunization period, both age groups of animals were infected with increasing numbers of a. suum eggs (500, 1,000, 2,000, 5,000, 10,000, and 20,000) at 7-day intervals while the pigs were receiving pyrantel tartrate in the feed. two days after the last infective dose, animals were placed on unmedicated feed for 8 days and then challeng ...19902352069
tumor necrosis factor-like cytotoxicity and anorexia in ascaris suum infected pigs.tumor necrosis factor-like cytotoxicity (tnf-lc) was examined in sera from 12 pigs experimentally infected with ascaris suum. the difference of tnf-lc levels between eight infected and four uninfected controls was not significant. when an endotoxin challenge was intravenously administered 1 month after the first dose of a. suum, the levels of tnf-lc in the sera of infected pigs were one-third that of the controls 125 min post-challenge (pc). in a more detailed study on four infected and two unin ...19902382388
biotin as a probe of the surface of ascaris suum developmental stages.sulfo-nhs-biotin (aqueous soluble) and nhs-biotin (organic soluble) labeled similar sds-2me (sodium dodecyl sulfate/beta-mercaptoethanol) soluble cuticular proteins of second stage larvae (l2) and third stage ascaris suum larvae (l3). comparable analysis of biotin-labeled fourth stage larvae (l4), young adults, and mature adult ascaris suum revealed strong labeling of several sds-2me soluble cuticular proteins with nhs-biotin, while sulfo-nhs-biotin appeared to strongly label a single sds-2me so ...19902385267
characterization of isolated porcine intestinal mucosal mast cells following infection with ascaris suum.porcine intestinal mucosal mast cells (immc) were isolated from intestinal tissues of swine by enzymatic digestion and density gradient separation. helminth-free swine and swine exposed to the nematode parasite, ascaris suum, were used as a source of intestinal tissue. up to 40% of the isolated intestinal cells stained metachromatically with toluidine blue ph 3.0, indicating the presence of immc. the histamine content of this cell population ranged from 2.9-8.9 pg per toluidine blue-positive imm ...19882462304
effects of the chitin synthesis inhibitor diflubenzuron on development of ascaris suum and haemonchus contortus.the potential of the chitin synthesis inhibitor diflubenzuron (dfb) to alter the development of the parasitic nematodes (ascaris suum and haemonchus contortus was investigated. dfb given orally (10 mg kg-1 per day for 30 days) to sheep inoculated with h. contortus infective larvae did not prevent the establishment of adults or affect fecal egg output. however, there was a significant (greater than 90%) decrease in the number of infective larvae recovered from fecal cultures derived from lambs ha ...19892505433
an abattoir survey of the prevalence of gastro-intestinal helminths and stephanurus dentatus in pigs in belize.in an abattoir survey in belize 137 pigs were examined for gastro-intestinal helminths and stephanurus dentatus. hyostrongylus rubidus, physocephalus sexalatus, globocephalus species and trichostrongylus colubriformis were recorded for the first time. ascarops strongylina, ascaris suum, macracanthorynchus hirudinaceus, strongyloides ransomi, trichuris suis, oesophagostomum species and s. dentatus were also found. oesophagostomum species (45%) and s. dentatus (42%) were the most prevalent. the pr ...19892529683
production and distribution of immunoglobulin-bearing cells in the intestine of young pigs infected with ascaris suum.pigs of 10 days-1 month old were orally infected with eggs of ascaris suum at different rates and inoculation schedules. histological sections from various parts of the small intestines were prepared to observe the production and localization of immunoglobulin-bearing cells. fluorescent antibody and immunoperoxidase staining methods were used to determine the number of igm-, iga- and igg-producing plasma cells in the intestinal lamina propria. significant increases in immunoglobulin-bearing cell ...19892531490
synergistic effect of migrating ascaris larvae and escherichia coli in piglets.the effect of intestinal flora on the establishment, development and pathogenicity of ascaris suum larvae in piglets (large white breed) was investigated. the infected piglets with ascaris and escherichia coli showed signs of pneumonia, cough with respiratory difficulties initially even though these moderated with time. they lost appetite and showed signs of unthriftiness with loss of weight. the packed cell volume was normal but the differential leucocyte counts of the pigs infected with ascari ...19892656846
effect of fenbendazole and pyrantel tartrate on the induction of protective immunity in pigs naturally or experimentally infected with ascaris suum.an experiment was conducted with 96 weanling pigs (avg initial wt 18.5 kg) divided into six treatment with two replicates of eight pigs each. pigs in treatments 1, 2 and 3 were penned in outside pens with dirt lots that previously were contaminated with a. suum ova to induce a natural ascaris infection. pigs in treatments 4, 5 and 6 were penned in an open-front building with solid concrete floors and were experimentally infected with 2,000 embryonated a. suum. ova on d 1, 15 and 29 of the experi ...19892722696
electron-transfer flavoprotein from anaerobic ascaris suum mitochondria and its role in nadh-dependent 2-methyl branched-chain enoyl-coa reduction.electron-transfer flavoprotein was purified to apparent homogeneity from mitochondria of the parasitic nematode, ascaris suum. the native molecular weight of the enzyme was 70,000, as estimated by gel filtration, and it migrated as two bands with apparent subunit molecular weights of 37,000 and 31,500 during sodium dodecylsulfate polyacrylamide gel electrophoresis. the enzyme exhibited an absorption coefficient for the bound fad of 13.5 mm-1.cm-1 at 436 nm and a protein/flavin (270 nm/436 nm) ra ...19892736251
some aspects of the pharmacology and physiology of the ascaris suum muscle.out of 11 agonists, including such agents as bradykinin, prostaglandin e2, eledoisin, only acetylcholine (ach) caused the isolated exposed ascaris muscle to contract during treatment for 30 sec and at 10(-7) g/ml (or lower concentrations). the possibility of using this preparation for the identification and quantification of ach in biological tissues and fluids was highlighted. neostigmine, but not physostigmine, augmented ach activity. this suggests that cholinesterases present in the ascaris m ...19892757466
influence of helminth parasite exposure and strategic application of anthelmintics on the development of immunity and growth of swine.infection of pigs with the intestinal roundworm parasite ascaris suum and strategic application of anthelmintic drugs during the growing phase of development were observed for specific effects on 1) development of immunity in feeder pigs and 2) growth rate during the finishing phase. management treatments included maintenance in a parasite-free concrete environment, maintenance in a concrete environment and inoculation with 1,000 infective a. suum eggs every other day over a 52-d period, and mai ...19892768122
efficacy of an in-feed ivermectin formulation against gastrointestinal helminths, lungworms, and sarcoptic mites in swine.the efficacy of ivermectin as an in-feed formulation was evaluated against naturally acquired gastrointestinal helminths, lungworms, and sarcoptic mites (experiment 1; n = 24) and against induced infection with intestinal nematodes (experiment 2; n = 24) in pigs. treatments consisted of ivermectin administered in feed at concentrations calculated to provide 100 or 200 micrograms/kg of body weight/d for 7 days or of nonmedicated feed (controls) for 7 days. at concentration of 100 micrograms of iv ...19892782722
nematode parasite infections of domestic pigs in a sub-tropical and high-rainfall area of india.fourteen hundred and ninety-six domestic pigs, sus scrofa domestica l., from a sub-tropical and high-rainfall area of india were examined over a period of 18 months to ascertain their nematode parasite spectrum. the eleven species recovered, arranged in descending order of prevalence, are as follows: ascaris suum, oesophagostomum dentatum, bourgelatia diducta, stephanurus dentatus, globocephalus connorfilii, physocephalus sexalutus, ascarops dentata, a. strongylina, pseudocruzia orientalis, seta ...19892787072
prevalence of helminth infections in swine in denmark.during 1982-1984 the occurrence of helminths in 66 sow herds was determined by examining faecal samples from swine of different age groups. ascaris suum was found in 88% of the herds, while oesophagostomum spp. and trichuris suis were found in 58 and 23% of the herds, respectively. strongyloides ransomi and hyostrongylus rubidus was not identified in any of the herds. the occurrence of both a. suum and oesophagostomum spp. was strongly related to the age of the animals. a. suum was most frequent ...19892815533
a unique cytoskeleton associated with crawling in the amoeboid sperm of the nematode, ascaris suum.nematode sperm extend pseudopods and pull themselves over substrates. they lack an axoneme or the actin and myosins of other types of motile cells, but their pseudopods contain abundant major sperm protein (msp), a family of 14-kd polypeptides found exclusively in male gametes. using high voltage electron microscopy, a unique cytoskeleton was discovered in the pseudopod of in vitro-activated, crawling sperm of the pig intestinal nematode ascaris suum. it consists of 5-10-nm fuzzy fibers organize ...19892910878
survey of internal parasites in western australian pig herds. 1. prevalence.between september 1982 and march 1984, 101 western australian piggeries with 15 or more sows were surveyed to determine the prevalence of internal parasites and examine the relationship between parasitism and management practices. faecal samples were collected from 20 pigs in 4 age groups in randomly selected piggeries, and examined for the presence of eggs of helminth parasites and protozoan cysts. evidence of nematode parasites was found in 79% of piggeries. sows were more commonly affected th ...19892930392
protective immunity to ascaris suum: analysis of swine peripheral blood cell subsets using monoclonal antibodies and flow cytometry.outbred domestic swine or sla inbred miniature swine were exposed to ascaris suum either naturally on contaminated lots or by inoculation with uv-irradiated attenuated eggs. both inbred and outbred swine developed virtually complete protection to a challenge of 10 000 eggs after natural exposure, but inbred swine were less resistant than outbred swine after uv-egg exposure. flow cytometric analysis of peripheral blood mononuclear cells from these animals, performed to determine changes in cell s ...19862939618
losses to internal parasites in swine production.experimental infections of pigs with strongyloides ransomi, stephanurus dentatus, ascaris suum, oesophagostomum spp. or trichuris suis at increasing levels generally decrease daily gain and increase feed to gain ratio linearly. at lower, subclinical levels of infection, the feed to gain ratio typically is increased 3% to 6% by an infection. based on low-level experimental infections with a. suum, the economic loss to producers in the u.s. from increased feed to gain ratio is estimated at $155 mi ...19882969380
efficacy of piperazine dihydrochloride against ascaris suum and oesophagostomum species in naturally infected pigs.a controlled trial was performed to evaluate the efficacy of piperazine dihydrochloride in a new granular formulation (ascarex d) against naturally occurring infections with ascaris suum, oesophagostomum dentatum and o quadrispinulatum. treatment effects were estimated on the basis of parasites recoverable from the intestinal contents. given orally at 200 mg per kg body weight the compound showed an efficacy of 99 to 100 per cent against a suum and the nodular worms. egg excretion of the respect ...19883046112
biochemical and immunological systematics of some ascaridoid nematodes: genetic divergence between congeners.vertical starch gel electrophoresis and trefoil immunodiffusion were used to study the systematics of some ascaridoid nematodes. within the ascarididae, the time scale of divergence was too great for intergeneric electrophoretic comparisons. congeneric electrophoretic comparisons of baylisascaris procyonis (host--raccoon) versus baylisascaris transfuga (host--black bear), and toxocara canis (host--domestic dog) versus toxocara cati (host--domestic cat) yielded nei genetic distance coefficients o ...19873114467
efficacy of flubendazole against gastrointestinal and lung nematodes in pigs.three trials were carried out on landrace pigs of various ages to assess the anthelmintic efficacy of flubendazole. the pigs were either artificially infected with metastrongylus apri or naturally or artificially infected with the gastrointestinal nematodes ascaris suum, oesophagostomum dentatum or hyostrongylus rubidus. for mass medication of young pigs and fatteners a dose regimen of 30 ppm flubendazole in the feed for 10 consecutive days was 100 per cent effective against the four nematode sp ...19883195017
effects of infection with enterobacteriaceae enteropathogens on subsequent infection with ascaris suum in the laboratory mouse.prior to infection with ascaris suum, icr strain mice were inoculated with salmonella typhisuis intraperitoneally or via gastric gavage. similarly, salmonella cholerae-suis var. kunzendorf, salmonella typhimurium and enterotoxigenic escherichia coli were administered to mice via gastric gavage 2 weeks prior to a. suum inoculation. previous inoculation with s. typhisuis, via the intraperitoneal or gastric gavage routes and s. cholerae-suis var. kunzendorf decreased recovery of ascarid larvae from ...19873296422
pulmonary bacterial deposition and clearance during ascarid larval migration in weanling pigs.pulmonary deposition and clearance of bacteria were measured in weanling pigs, half of which had been inoculated at age 31 days with larvated ascaris suum ova. seven days later, when breathing signs of larval migration were pronounced, all pigs were exposed to aerosolized escherichia coli (strain b). then, either immediately after aerosol exposure (for deposition assessment) or immediately after a 120 minute period in filtered air (for clearance), bacteria in the pigs' lungs were counted. ascari ...19873330966
ascaris suum antigens incorporated into liposomes used to stimulate protection to migrating larvae.four- to 8-week-old spf pigs were immunized, using antigens of ascaris suum incorporated into liposomes, via intestinal cannula or orally. avridine was also incorporated in the liposomes in one experiment and interleukin-2 (il-2) injected into pigs in another experiment. a priming dose of embryonate eggs (80-470 eggs/pig) were given in four of six experiments. compared to control animals, the greatest protection of pigs to migrating ascarid larvae from a challenge dose of 10,000 embryonated eggs ...19883347994
ascaris suum: development of intestinal immunity to infective second-stage larvae in swine.the development of protective immunity to ascaris suum was examined in pigs naturally exposed to eggs on a contaminated dirt lot. pigs became almost totally immune to second-stage larvae migrating from the intestines because few larvae from a challenge inoculum could be found in the lungs, and liver white-spot lesions (an immunopathologic response to migrating larvae) were absent. blood from these pigs contained lymphocytes that responded blastogenically to larval antigens in vitro, while the se ...19883366215
[three recent cases of ascariasis in northern kyushu].ascariasis is considered to be one of the rare infectious diseases in japan, but recently it has been slightly increasing. this paper reports three ascariasis cases who seemed to be infected recently in the kitakyushu area, japan. case 1: a 59-year-old woman in kitakyushu city passed a round worm after continuous abdominal pain. the patient was discharged from the hospital because of no further abnormal intestinal symptoms and findings. case 2: an 85-year-old woman in nakama city, who suffered f ...19883368668
developmental changes in cuticular proteins of ascaris suum.1. cuticles were isolated from developmental stages of the swine nematode ascaris suum by a combination of mechanical disruption and detergent treatment of larvae or by surgical removal of cuticle from adults. proteins from the isolated cuticles were solubilized with 2-mercaptoethanol (2me) and analyzed by sds-page. 2. 2me soluble, cuticular proteins from adults consisted of 5 to 6 bands with 80% of proteins in 2 bands with mol. wts of 106,000 and 93,000. cuticular proteins from the third and fo ...19883409662
histopathological changes in organs of the miniature pigs gtt infected with invasive larvae of ascaris suum goeze, 1782. 19873436470
demonstration of carboxyl and thiol protease activities in adult schistosoma mansoni, dirofilaria immitis, angiostrongylus cantonensis and ascaris suum.evidence has been presented showing two kinds of acidic protease activities in adult schistosoma mansoni, dirofilaria immitis, angiostrongylus cantonensis and ascaris suum. a haemoglobinolytic activity without adding any sh-containing compounds was maximal at ph 3.5, 2.5, 3.0 and 3.5 in s. mansoni, d. immitis, angiostrongylus cantonensis and ascaris suum respectively. the inhibitor studies demonstrated that this activity is ascribable to carboxyl protease(s). in the presence of dithiothreitol, a ...19863517135
efficacy, safety, and residue evaluation of levamisole gel formulation in sows.anthelmintic efficacy, safety, and residue studies were conducted in sows and gilts with a levamisole gel containing 11.5% levamisole hcl. in 12 sows and 12 gilts, 8 mg of levamisole hcl equivalent/kg of body weight orally was 100% (resinate) and 91.1% (gel) effective against 55-day-old ascaris suum and 100% (gel) and 96.1% (resinate) effective against oesophagostomum dentatum. in 20 sows given levamisole gel (8 mg of levamisole hcl/kg) orally just before breeding, 4 to 6 weeks after breeding, 4 ...19873592389
detection of complement fixation antibody against ascaris suum antigen in pigs with white spots in the liver. 19873613356
homology and heterology between the secreted antigens of the parasitic larval stages of ascaris lumbricoides and ascaris suum.the materials released in vitro by the tissue-parasitic larval stages of the large roundworm of man, ascaris lumbricoides, were analysed by radio-iodination, immunoprecipitation, and sds-page. the antigens were found to be heterogeneous, ranging in molecular weight from 14 to 410 kd, and were found to alter radically during the parasites' migration to the lungs. the antigens secreted by the infective and lung-stage larvae of the pig homologue, ascaris suum, were compared with those of the human ...19873621674
efficacy of dichlorvos, fenbendazole, and ivermectin in swine with induced intestinal nematode infections.anthelmintic efficacies of dichlorvos, fenbendazole, and ivermectin were compared in specific-pathogen-free crossbred weanling pigs inoculated with ascaris suum, trichuris suis, and oesophagostomum dentatum. on postinoculation day (pid) 50, 24 pigs in each treatment group were treated orally with 43 mg of dichlorvos/kg of body weight, 3 x 3 mg of fenbendazole/kg, or 300 micrograms of ivermectin/kg, sc. twenty-four pigs were not treated. on posttreatment day 7 (pid 57), 12 pigs from each treatmen ...19873631712
identity and tissue localization of free and conjugated ecdysteroids in adults of dirofilaria immitis and ascaris suum.adult males and females of the dog heartworm, dirofilaria immitis, and of the swine parasite, ascaris suum, were extracted, the free and polar conjugated ecdysteroid fractions separated and the latter hydrolysed enzymically. the ecdysteroids released by hydrolysis of the conjugates and the free hormones were analysed by radioimmunoassay, high-performance liquid chromatography on reversed phase and adsorption columns monitoring fractions by radioimmunoassay, and by gas-liquid chromatography/mass ...19873670345
parasite transmission in confined hogs.examinations of fecal specimens from swine of all ages maintained in a totally enclosed confinement facility in south georgia were conducted in a 5-year survey (1977-1981) to determine the prevalence, intensity, and transmission patterns of intestinal nematode and protozoan parasites. weaned pigs in the nursery had no detectable parasitic infections except sporadic isospora suis in newly weaned pigs. growing-finishing hogs had a low prevalence of ascaris suum, oesophagostomum spp. and balantidiu ...19863705423
[effect of selected pesticides on the development and invasiveness of the ova of ascaris suum goeze, 1782]. 19863705585
[influence of the migration of ascaris suum larvae on blood parameters in the pig].biochemical and hematological blood parameters were investigated in pigs experimentally infected with ascaris suum. the most marked changes were recorded on day 5-8 post inoculation, during fever. in this period there was a marked decrease of concentrations of albumin, iron, thyroxin, a and e vitamins in blood serum, while compared with the control group the contents of a vitamin in liver did not change. further, the concentrations of nonesterified fatty acid increased, as well as those of inorg ...19863717687
ascaris suum infection in pigs sensitizes lymphocytes but suppresses their responsiveness to phytomitogens.the effect of ascaris suum infection and treatment with fenbendazole on the blastogenic response of peripheral blood lymphocytes to a. suum antigens and to three phytomitogens was assayed by the lymphocyte transformation technique. repeated infections with a. suum led to the development of sensitized lymphocytes primarily responding to egg hatching fluid antigen. treatment with fenbendazole decreased the number of specific sensitized lymphocytes, but favorably increased the resistance of pigs to ...19863727343
susceptibility of fourth-stage ascaris suum larvae to fenbendazole and to host response in the pig.fenbendazole given at the rate of 2.5 g/kg of feed for 3 days had 100% efficacy against 4th-stage ascaris suum larvae in 8 pigs. eight control pigs had a total of 108 a suum. in 6 pigs infected 3 times with 3rd-stage a suum larvae and treated with fenbendazole after the larvae molted to the 4th stage, the challenge exposure-derived population was decreased by 64%. similar sequential infections in 6 pigs similarly infected, but not treated with fenbendazole, decreased the challenge exposure-deriv ...19863752674
the effect of pre-farrowing treatment of the sow with ivermectin on the early establishment of ascaris suum and oesophagostomum spp in the progeny.residual infective ascaris suum eggs in farrowing pens provide the initial source of infection for piglets under swedish management conditions. in such circumstances there is little evidence to support pre-farrowing treatment of the sow specifically against a. suum. the present study shows, however, that ivermectin-treatment of the sow prevents the early establishment of a. suum and oesophagostomum spp in her litter.19863755816
[quantitative comparison of amino acids in adult proteins of ascaris lumbricoides, ascaris suum and toxocara canis]. 19863770717
immunity of swine to ascaris suum.swine were hyperimmunized to ascaris suum by giving multiple oral inoculations of embryonated eggs. sera and lymphocyte lysate from these pigs were administered parenterally to 4-week-old pigs. the latter animals were no more resistant to larval migration than control pigs receiving sera or lymphocyte lysate from non-immunized pigs. other pigs were infected with transmissible gastroenteritis (tge) virus, allowed to recover and challenged with embryonated ascarid eggs. they likewise were no more ...19863788028
preliminary communication on cryptosporidiosis of pigs in viet-nam.feces were collected from 17 pigs (aged 4-8 weeks) in several farms situated within the region of hanoi. by coproscopy of the samples, eggs of ascaris suum and oesophagostomum dentatum were detected as well as oocysts of eimeria scabra, e. debliecki, e. perminuta and e. suis, and in addition to it, cryptosporidia to an extend indicating a considerable incidence of this parasitic protozoan in the country.19863804081
ascaris suum: biosynthesis and isoinhibitor profile of chymotrypsin/elastase isoinhibitors.chymotrypsin/elastase isoinhibitors were radiolabeled when live, adult ascaris suum were incubated in tissue culture medium (nctc-135) supplemented with l-[35s]cysteine. this is the first demonstration that the synthesis of these proteins occurs in a. suum; the isoinhibitors are not host products utilized by the parasite against its host. of five chymotrypsin/elastase isoinhibitors demonstrable in a. suum, only isoinhibitors 1, 4, and 5 were found in each worm. the amino acid sequences of these ...19853846534
[digestibility of nutrients in a feed mixture in certain swine helminthiases].the average digestibility coefficients of feed mixture nutrients in pigs infected medium severely to severely by the helminths ascaris suum and oesophagostomum dentatum were lower in comparison with the post-dehelminthization condition (dry matter by 3.1%, crude protein by 6.7%, fat by 8.9%, nitrogen-free extracts and fibre by 2.2%, organic matter by 3.1%). the content of total digestible nutrients made 679 g in infected pigs and 708 g in dehelminthized pigs. in the period following the experime ...19853931335
comparative purification and characterization of invertebrate muscle glycogen synthase from the porcine parasite ascaris suum.glycogen synthase has been purified from the obliquely striated muscle of the swine parasite ascaris suum. the muscle contains a concentration of glycogen synthase and glycogen which is 20-fold and 15-fold, respectively, greater than rabbit skeletal muscle. the enzyme could not be solubilized with salivary amylase, but partial solubilization was achieved by activation of endogenous phosphorylase. the enzyme was purified to 85-90% homogeneity (specific activity = 4.3 units/mg) by deae-cellulose, ...19853934170
influence of an experimental infection of ascaris suum on performance of pigs.thirty-two pigs (average 26.6 kg live weight) were individually housed and fed to study the effect of an infection of ascaris suum (either 0, 600, 6,000 or 60,000 a. suum eggs/pig) on performance of growing-finishing pigs. increasing the level of a. suum infection produced linear (p less than .07) and quadratic (p less than .09) effects on final weight, weight gain and average daily gain. feed to gain ratio and number of a. suum worms recovered from the intestines of pigs at slaughter increased ...19853972742
steroidal enhancement of growth in parasitic larvae of ascaris suum: validation of a bioassay.third-stage larvae of the swine parasitic roundworm, ascaris suum, were grown in stationary multiwell cultures in the presence of synthetic steroid hormones or the hplc fractions of a steroid extract of adult female reproductive tracts to determine the subsequent effect of these compounds on length of 4th-stage larvae. log doses of ecdysone, 20-hydroxyecdysone, progesterone, and makisterone standards, from 50 pg/ml to 500 ng/ml, were incubated with larvae for 1 day in rpmi-1640 supplemented with ...19853973553
the effect of continuous in-feed medication with thiophanate on ascaris suum and oesophagostomum spp. egg output in young pigs.the full potential of anthelmintics now available for single dose treatment is not achieved because the devising system for worm control in piglets/weaners is not efficiently applicable in practice. therefore an in-feed medication programme for growing young pigs, allowing only one feed lot to be handled by the farmer, was tested in two studies. study a feed containing 0.0225% thiophanate was continuously fed almost ad lib. to piglets from birth right up to about 25 kg body weight when ready for ...19854000918
taenia hydatigena: a cause of persistent liver condemnations in lambs.a problem of high levels of liver condemnations which had affected bought-in lambs fattened on a north yorkshire farm for several years was investigated. because the farmer spread pig slurry on the land, migrating ascaris suum larvae were initially suspected as a cause but the investigation eliminated this possibility. migrating taenia hydatigena larvae were found in tissue sections and isolated from livers. a combination of parasitological and serological evidence using oncospheral antigen in a ...19854002589
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