| identification of an immunologically distinct papillomavirus from lesions of epidermodysplasia verruciformis. | virions isolated from warts of 2 siblings with epidermodysplasia verruciformis (ev), a rare disease characterized by the lifelong growth of warty skin tumors containing papova-like virions, were compared to isolates of human papillomavirus (hpv) from 3 pools of plantar and common hand warts. the length of relaxed, circular (form ii) molecules of ev virion dna approximated the length of hpv dna but was 3.3% longer. antisera prepared in rabbits against the 3 hpv pools coated and aggregated hpv in ... | 1977 | 71354 |
| primary tissue culture of human wart-derived epidermal cells (keratinocytes). | the formation of human skin warts may depend on transformation by human papilloma virus or result from a productive virus infection. to explore this problem, methods were developed for the primary tissue culture of epidermal cells (keratinocytes) derived from wart tissues and normal skin. wart-derived keratinocytes continued to proliferate for periods exceeding 3 months, but the patterns of cell growth were identical to normal keratinocytes. electron microscopic and immunohistologic examination ... | 1975 | 164558 |
| human papillomavirus dna: physical map. | human papillomavirus (hpv) dna form i (supercoiled) was prepared from plantar warts. hpv dna was cleaved with restriction enzymes obtained from the following sources: escherichia coli (ecori), hemophilus influenzae strain rd (both unfractionated hind and aeparated hindii and hindiii enzymes) and hemophilus parainfluenzae (hpai). the cleavage products were analyzed by polyacrylamide gradient slab gel electrophoresis and electron microscopy. hpv dna was cleaved into two fragments by ecori (87% and ... | 1975 | 174077 |
| [human papilloma virus (author's transl)]. | | 1976 | 189105 |
| chromatin-like structures obtained after alkaline disruption of bovine and human papillomaviruses. | four low-molecular-weight polypeptides migrating like h2a, h2b, h3, and h4 calf liver histones were detected by sodium dodecyl sulfate-acrylamide gel electrophoresis of highly purified preparations of bovine papillomavirus (bpv) and human papillomavirus (hpv). complexes of these polypeptides and viral dna were isolated by agarose-gel filtration of the alkaline disruption products of both viruses. when observed under the electron microscope, these complexes appeared as circular structures compose ... | 1977 | 191643 |
| chromatin-like structures obtained after alkaline disruption of bovine and human papillomaviruses. | four low-molecular-weight polypeptides migrating like h2a, h2b, h3, and h4 calf liver histones were detected by sodium dodecyl sulfate-acrylamide gel electrophoresis of highly purified preparations of bovine papillomavirus (bpv) and human papillomavirus (hpv). complexes of these polypeptides and viral dna were isolated by agarose-gel filtration of the alkaline disruption products of both viruses. when observed under the electron microscope, these complexes appeared as circular structures compose ... | 1977 | 191643 |
| human papillomavirus dna: physical mapping of the cleavage sites of bacillus amyloliquefaciens (bami) and haemophilus parainfluenzae (hpaii) endonucleases and evidence for partial heterogeneity. | the dna of human papillomavirus (hpv) obtained from a pool of plantar warts is cleaved by bacillus amyloliquefaciens (bami) and haemophilus parainfluenzae (hpaii) restriction endonucleases at one and four specific sites, respectively. these sites were localized on the previously established cleavage map of hpv dna, using the hind, hindiii, hpai, and ecori endonuclease restriction sites as reference. the four hpaii sites were mapped, clockwise, at 1.4, 41.1, 44.3, and 52.8% of the genome length f ... | 1977 | 191644 |
| identification and characterization of a papillomavirus from birds (fringillidae). | from skin papillomas of the chaffinch (fringilla coelebs), a virus has been purified and studied by physicochemical techniques and electron microscopy. the virions measure 52 nm in diameter and are composed of 72 morphological units arranged in a skew t = 7d surface lattice. a sedimentation coefficient of about 300s and a buoyant density of 1.34 g/ml in cscl were determined for the particle. its protein composition resembles that of human papillomavirus, and the circular double-stranded genome m ... | 1977 | 197046 |
| inverted repetitive sequences in human papilloma virus 1 (hpv-1) dna. | | 1977 | 201085 |
| characterization of human papilloma virus (h.p.v.) present in genital warts. | | 1978 | 205177 |
| so-called multicentric pigmented bowen's disease. report of a case and a possible etiologic role of human papilloma virus. | multicentric pigmented bowen's disease (mpbd) is a bowenoid atypia in the genitocrural region with peculiar clinical appearances. a 36-year-old japanese female patient showed a variety of lesions. clinically the lesions on the external genitalia consisted of brown-black papillomatous eruptions, black discrete or confluent papules, and whitish macerated papules. histologically only black papules showed bowenoid atypia, but whitish papules also showed transient bowenoid atypia. electron microscopi ... | 1978 | 211056 |
| seroepidemiologic studies of bovine papillomavirus infections. | bovine and human sera were analyzed for the presence of antibodies against bovine papillomavirus types 1 and 2 (bpv 1 and 2) and human papillomavirus type 1 (hpv 1) in a solid-phase radioimmunoassay. human sera did not react with bpv antigens, and bovine sera showed no evidence of antibodies against hpv 1. in contrast, 19% of all bovine sera tested reacted with bpv 1 and 2 antigens, and 35% of human sera revealed antibodies against hpv 1. no serologic evidence was obtained for heterologous infec ... | 1979 | 220453 |
| seroepidemiologic studies of bovine papillomavirus infections. | bovine and human sera were analyzed for the presence of antibodies against bovine papillomavirus types 1 and 2 (bpv 1 and 2) and human papillomavirus type 1 (hpv 1) in a solid-phase radioimmunoassay. human sera did not react with bpv antigens, and bovine sera showed no evidence of antibodies against hpv 1. in contrast, 19% of all bovine sera tested reacted with bpv 1 and 2 antigens, and 35% of human sera revealed antibodies against hpv 1. no serologic evidence was obtained for heterologous infec ... | 1979 | 220453 |
| localization of viral dna-replication in sections of human warts by nucleic acid hybridization with complementary rna of human papilloma virus type 1. | using complementary rna of human papilloma virus type 1 (hpv1) and in situ hybridization techniques the localization of viral dna replication was studied in sections of 38 human virus acanthomata from 31 different patients. in five cases significant labeling was detected by autoradiography. labeling started always in the first or the second suprabasal cell layer and was strongly limited to the nuclei. a remarkable early beginning of the vacuolated process seemed to be correlated with the visible ... | 1979 | 220921 |
| connection between capsomeres in human papilloma virus. | | 1979 | 223316 |
| characterization of human papillomavirus 3 in warts of a renal allograft patient. | multiple flat wart-like lesions of a renal allograft recipient were shown to contain hpv 3 or a serologically crossreacting virus by indirect immunofluorescence with monospecific animal antisera against hpv [1--5]. the patient's serum revealed virus specific antibodies (immunofluorescence titer 1/80). papillomaviruses were isolated and after in vitro iodination 3 major proteins (mw 70.000, 56.000 and 43.000) were detected by sds polyacryalmide gel electrophoresis. dna was extracted from the wart ... | 1979 | 227966 |
| immunology of human warts. | rapid advances have occurred in the characterization of human papilloma virus (hpv) types applying the new advanced techniques of restriction endonuclease analysis and molecular hybridization to human wart virus. human papilloma virus can no longer be viewed as a single, homogeneous virus producing all varieties of clinical warts. at least three antigenically heterogeneous hpv types have been associated with common and plantar warts. two additional hpv types have been found in patients with epid ... | 1979 | 229134 |
| detection of human papillomavirus in the prostate by polymerase chain reaction and in situ hybridization. | human papillomavirus is associated with a variety of anogenital lesions, including genital warts, precancers and cancers. in male patients human papillomavirus has been identified in proliferative lesions ranging from penile and urethral warts to penile and prostatic cancers. we examined the association of human papillomavirus deoxyribonucleic acid (dna) in 84 prostate tissue specimens. specimens were selected from radical prostatectomy, transurethral resection or transrectal biopsy procedures. ... | 1992 | 1279224 |
| prognostic factors in invasive cervical carcinomas associated with human papillomavirus (hpv). quantitative data and cytokeratin expression. | as a part of a larger programme to search for the prognostic factors in cervical cancer, quantitative morphometry, demonstration of agnors and expression of different cytokeratin polypeptides (sk2-27, sk1, a 53-b/a2) were used to study a series of 85 cervical squamous cell carcinomas, previously analysed for the presence of human papillomavirus (hpv) dna by in situ hybridization and polymerase chain reaction (pcr). the following nuclear profile parameters were calculated: nuclear area, perimeter ... | 1992 | 1280356 |
| human papillomavirus in oral verrucal-papillary lesions. a comparative histological, clinical and immunohistochemical study. | the purpose of this study was to determine the presence of human papillomavirus (hpv) virions in the verrucal-papillary (ovp) lesions by clinical, histological and immunohistochemical [peroxidase-antiperoxidase (pap) features]. thirty seven positive cases were compared with 47 negative cases. considerable overlapping of clinical and histological features can be seen among all these lesions. the study provides evidence that there are no clinical or histologic features with taken alone are strongl ... | 1992 | 1281537 |
| [interferon, interferon inducers, human papillomavirus infection]. | | 1992 | 1285258 |
| evaluation and follow-up of abnormal pap smears. | an estimated 13,500 cases of invasive cervical cancer and 6,000 related deaths occur each year in the united states. these numbers can be significantly reduced if all women are regularly screened with papanicolaou (pap) smears and undergo colposcopy when abnormalities are detected. screening with pap smears should begin at age 18 or at the age of first sexual intercourse and should be repeated every one to three years, depending on individual risk factors, until age 65. screening may be disconti ... | 1992 | 1309403 |
| clinical effects of photodynamic therapy on recurrent laryngeal papillomas. | thirty-three patients with moderate to severe recurrent laryngeal papillomatosis underwent photodynamic therapy at our institution. all received 2.5 mg/kg of dihematoporphyrin ether intravenously either 48 or 72 hours prior to photoactivation with an argon pump dye laser system. photosensitivity was the only side effect seen. statistical analysis showed a significant decrease, by approximately 50%, in the average rate of laryngeal papilloma growth following treatment. the response was especially ... | 1992 | 1309420 |
| the comparative test performance of dot filter hybridization (viratype) and conventional morphologic analysis to detect human papillomavirus. | to investigate the test performance of a commercially available detection kit for human papillomavirus (hpv), the relationship between the detection of hpv by dot filter hybridization (dfh) and by standard morphologic methods was studied. four hundred two cervical samples taken from 381 patients referred to a colposcopy clinic were examined. human papillomavirus dna sequences were identified and typed using commercially available anti-sense rna probes. simultaneous cytologic smears were obtained ... | 1992 | 1309483 |
| antibody-mediated neutralization of human papillomavirus type 11 (hpv-11) infection in the nude mouse: detection of hpv-11 mrnas. | the nu/nu mouse xenograft is the only experimental system permitting the growth of human papillomaviruses (hpv). previous studies demonstrating inhibition of hpv-11 infection by antibodies against hpv-11 virions have used indirect markers of infection, such as graft size and histopathologic features. the presence of hpv-11 mrnas was used as a direct marker of infection: infectious hpv-11 was incubated with rabbit serum raised against purified hpv-11 virions or with the corresponding preimmune se ... | 1992 | 1309849 |
| human papillomavirus type 1 e4 proteins differing by their n-terminal ends have distinct cellular localizations when transiently expressed in vitro. | two major human papillomavirus type 1 (hpv-1) e4 proteins are found in large amounts in productively infected differentiating wart cells, a 17-kda protein translated from an e1-e4 transcript and a processed 16-kda protein lacking the e1 amino acids at least. the functions of the e4 proteins are still unknown. we have designed an in vitro system allowing the transient expression of three forms of hpv-1 e4 proteins: the 17-kda e1-e4 protein, an e4 protein without the five e1 amino acids (e4-3200), ... | 1992 | 1309915 |
| a comparison of risk factors in juvenile-onset and adult-onset recurrent respiratory papillomatosis. | the clinical triad of a firstborn delivered vaginally to a young (teenage) mother has been previously noted among juvenile onset recurrent respiratory papillomatosis (jo-rrp) patients. this study was based on a questionnaire survey of jo-rrp patients, adult onset recurrent respiratory papillomatosis (ao-rrp) patients, and juvenile and adult controls. the survey results revealed that the complete or partial triad was observed in 72% of jo-rrp patients, 36% of ao-rrp patients, 29% of juvenile cont ... | 1992 | 1309932 |
| amplification of human papillomavirus type 16 transforming genes from cervical cancer biopsies and lymph nodes of hungarian patients. | we have used a polymerase chain reaction (pcr) to examine cervical cancer biopsy specimens and pelvic lymph nodes for the presence of human papillomavirus type 16 (hpv 16) dna. of the 75 cervical specimens tested, 36 (48%) were positive for hpv 16 in the pcr. a total of 65 pelvic lymph nodes removed during radical surgery on 35 women were also analyzed. lymph nodes originating from 19 patients whose cervical biopsy specimens were negative for hpv 16 seemed to lack hpv 16 sequences. for 16 women ... | 1992 | 1310330 |
| human papillomavirus--related diseases in the female patient. | human papillomavirus (hpv) has been strongly associated with malignancy in the female lower genital tract. because squamous-cell carcinoma of the cervix is preceded by a spectrum of easily detectable and treatable premalignant changes, it is very preventable. the management of the patient with an abnormal papanicolaou smear and the treatment of cervical, vaginal, and vulvar disease are outlined. | 1992 | 1310548 |
| koilocytosis in neoplasia of the urinary bladder. | koilocytosis is commonly regarded as indicative of human papilloma virus infection in the uterine cervix. in 1987 morphologically similar changes were reported in bladder tumours. this is confirmed in the present study, the incidence here being 65%. in addition the incidence of koilocytic change was shown here to increase from non-infiltrative who grade i to infiltrative who ii lesions, and to be more common in bladder lesions in women with cervical koilocytosis. the latter is in keeping with ou ... | 1992 | 1310631 |
| human papillomavirus type 16 e5 gene stimulates the transforming activity of the epidermal growth factor receptor. | we have until recently made several unsuccessful attempts to assign any activity to the human papillomavirus type 16 (hpv-16) e5 gene product. however, studies with the bovine papilloma virus 1 (bpv-1) e5 protein indicated an interaction with the epidermal growth factor receptor (egfr). in light of the overall similarity between the hpv and bpv e5 proteins we attempted to determine whether the hpv-16 e5 gene had any common activity. in cells expressing high levels of egfr plus hpv-16 e5 we found ... | 1992 | 1311063 |
| recent progress in defining the epidemiology of human papillomavirus infection and cervical neoplasia. | | 1992 | 1311392 |
| human papillomavirus dna detection in papanicolaou-stained cervical smears with a nonradioactive, in situ hybridization assay. | archived papanicolaou-stained cervical smears from women with different cervical pathologies were processed for human papillomavirus (hpv) dna detection and typing with an in situ hybridization (ish) assay that employed commercial biotinylated hpv dna probes. two hpv dna probes were utilized: one included hpv genotypes 6/11 and the other, 16/18. the method yielded positive results for hpv dna 6/11 in 5 cases with condylomata acuminata (100%) and in 2 of 47 with flat warty lesions (4.2%), whereas ... | 1992 | 1311885 |
| clinical applications of molecular biologic screening for human papillomavirus: diagnostic techniques. | | 1992 | 1311996 |
| prevalence of human papillomavirus types 16 and 18 in squamous-cell carcinoma of the penis: a retrospective analysis of primary and metastatic lesions by differential polymerase chain reaction. | human papillomaviruses (hpv), particularly types 16 and 18, may be carcinogenic effectors in a variety of human lower-genital-tract malignancies. using the highly sensitive technique of differential polymerase chain reaction (d-pcr) with amplimers from the e6 open reading frames of hpv types 16 and 18, a retrospective analysis of a 20-year institutional experience with squamous-cell carcinoma of the penis (sccp) was performed to determine the prevalence of these hpv types in this malignancy. par ... | 1992 | 1312062 |
| seroreactivity against hpv 16 e4 and e7 proteins in renal transplant recipients and pregnant women. | | 1992 | 1312108 |
| antibodies to hpv-16 e6 and e7 proteins as markers for hpv-16-associated invasive cervical cancer. | transforming proteins e6 and e7 of human papillomaviruses (hpvs) are consistently expressed in hpv-associated cervical cancers. in elisa with four hpv-16 e6-e7 peptides, patients with hpv-16-associated invasive cervical cancer (group 1) had a greater seroreactivity than all other groups, which included patients with hpv-16-associated cervical intraepithelial neoplasia, invasive cervical cancer patients without hpvs, and unaffected controls. a larger proportion of group 1 sera, as compared to ser ... | 1992 | 1312268 |
| molecular variants of human papillomavirus type 16 from four continents suggest ancient pandemic spread of the virus and its coevolution with humankind. | we have amplified by the polymerase chain reaction, cloned, and sequenced genomic segments of 118 human papillomavirus type 16 (hpv-16) isolates from 76 cervical biopsy, 14 cervical smear, 3 vulval biopsy, 2 penile biopsy, 2 anal biopsy, and 1 vaginal biopsy sample and two cell lines. the specimens were taken from patients in four countries--singapore, brazil, tanzania, and germany. the sequence of a 364-bp fragment of the long control region of the virus revealed 38 variants, most of which diff ... | 1992 | 1312620 |
| the e6 and e7 genes of human papillomavirus type 6 have weak immortalizing activity in human epithelial cells. | previous studies have shown that the e7 gene of human papillomavirus (hpv) type 16 or 18 alone was sufficient for immortalization of human foreskin epithelial cells (hfe) and that the efficiency was increased in cooperation with the respective e6 gene, whereas the hpv6 e6 or e7 gene was not active in hfe. to detect weak immortalizing activities of the hpv6 genes, cells were infected with recombinant retroviruses containing hpv genes, alone and in homologous and heterologous combinations. the hpv ... | 1992 | 1312623 |
| nucleotide 880 splice donor site required for efficient transformation and rna accumulation by human papillomavirus type 16 e7 gene. | mutations within coding sequences of the various human papillomavirus type 16 (hpv-16) genes have been used to demonstrate that the hpv-16 e7 gene is necessary and sufficient for transformation of rodent cells. we now provide evidence that, in addition to e7 coding sequences, a small cis-acting region immediately flanking the 3' end of e7 coding sequences is also required for transformation. this was shown by translation termination linker insertion, progressive deletion analysis, and site-direc ... | 1992 | 1313897 |
| replication of plasmid-derived human papillomavirus type 11 dna in cultured keratinocytes. | plasmid-derived human papillomavirus type 11 (hpv11) dna has been shown to replicate episomally and semiconservatively following transfection of primary human foreskin keratinocytes. hpv11 dna was excised from the bacterial plasmid, religated to form circular molecules, and cotransfected along with psv2neo. transfectants were selected and shown to contain replicated episomal hpv dna. once selected, hpv11 dna persists in cells through at least two additional passages. | 1992 | 1313926 |
| differential cooperation of a carcinogen with human papillomavirus type 6 and 16 dnas in in vitro oncogenic transformation. | in contrast to the strong association of human papillomavirus (hpv) type 16 with genital malignancies, hpv 6 has been found essentially in benign genital lesions. in these studies we show that hpv type 6 and 16 dnas behave differently also in their ability to transform nih 3t3 cells in cooperation with the carcinogen n-methyl-n'-nitro-n-nitrosoguanidine (mnng). although we could show that both hpv-6- and hpv-16-transfected genomes were integrated and expressed in nih 3t3 cells, only the nih 3t3 ... | 1992 | 1314784 |
| detection of hpv dna in archival specimens of cervical cancer using in situ hybridisation and the polymerase chain reaction. | an archival survey of 98 cervical cancer specimens dating from the 1920s to the 1980s was undertaken to determine whether changes had occurred in the prevalence of human papilloma-virus (hpv) dna. hpv dna was detected in paraffin sections of cancers fixed in 10% formalin by in situ hybridisation (ish) using hpv 6, 11, 16, and 18 32p-labelled dna probes under conditions of high stringency; and by the polymerase chain reaction (pcr) using 20-mer oligonucleotide primers to amplify 109 bases of the ... | 1992 | 1315371 |
| human papillomavirus infection of the uterine cervix. tissue sampling and laboratory methods affect correlations between infection rates and dysplasia. | two common tissue sampling techniques--colposcopic biopsy and cervical scrape--and two common human papillomavirus (hpv) detection techniques--southern blot and dot blot (sb and virapap [vp])--were compared to determine whether differences in these techniques alter correlations between "oncogenic" hpvs and cervical neoplasia. in 87 women with persistently abnormal papanicolaou (pap) smears, concurrent biopsy and scrape specimens contained hpv in 21 (24%) and contained no hpv in 26 (30%); 30 scra ... | 1992 | 1315486 |
| a placebo controlled observer blind immunocytochemical and histologic study of epithelium adjacent to anogenital warts in patients treated with systemic interferon alpha in combination with cryotherapy or cryotherapy alone. | to examine biopsy specimens of tissue immediately adjacent to anogenital (ag) warts which had been treated with either cryotherapy plus subcutaneous interferon (ifn) alpha 2a or cryotherapy alone, for histological features of (a) human papilloma virus (hpv) infection (b) localised cellular immune responses, to further characterise any cellular immune infiltrates with tissue immunocytochemistry, and to relate any histological, immunocytochemical findings to the treatment response of nearby ag war ... | 1992 | 1316307 |
| effect of human papillomavirus infection on estrogen receptor and heat shock protein hsp27 phenotype in human cervix and vagina. | in this study we have explored whether, as a consequence of human papillomavirus (hpv) infection, there is inappropriate expression of estrogen receptor and/or of a small heat shock protein of 27,000 daltons (hsp27). estrogen receptor, hsp27, and hpv structural antigens were detected by immunocytochemistry, while hpv dna (6/11, 16/18, 31/35/51) was determined by in situ hybridization in cervical and vaginal samples from 40 patients. most of the samples with hpv infection without atypia showed a ... | 1992 | 1316321 |
| papilloma virus infection. precancer and epidermoid cancer. | we present a review of the relationship between human papillomavirus (hpv), precancer and epidermoid cancer of the uterine cervix, co-factors that contribute in cervical carcinogenesis and the mechanism through which papillomaviruses transform normal cells into neoplastic cells by inducing an overproduction of the encoded proteins. we also offer final remarks and conclusions on this pathology that increases its incidence every year. | 1992 | 1316842 |
| detection of human serum antibodies that neutralize infectious human papillomavirus type 11 virions. | a selection of human sera were tested for the presence of antibodies that neutralized infectious human papillomavirus (hpv) type 11. neutralizing antibodies were detected by prevention of hpv-11-induced condylomatous transformation of human foreskin chips transplanted subrenally into athymic mice. test sera were obtained from 21 female patients with genital condylomas and eight patients with laryngeal papillomas. control patients consisted of 57 adult random blood donors and five asymptomatic ch ... | 1992 | 1316943 |
| what's new in diagnosis and treatment of hpv-associated cervical lesions. | the human papilloma virus (hpv) has been subject to intense discussion during the last decade, as evidence has accumulated to strongly suggest that viruses play an essential part in the genesis of cervical neoplasms. the contamination rate of the female population is reported to range between 5 and 80%, depending on the modality of detection. many indications almost reliably suggest the oncogenicity of certain hpv-types. the highest progression rate was found in hpv 16 lesions, followed by hpv 1 ... | 1992 | 1317558 |
| papillomavirus found in anorectal squamous carcinoma, not in colon adenocarcinoma. | we used polymerase chain reaction dna amplification methods for the detection and typing of genital human papillomaviruses in paraffin-embedded tissue sections of five patients with anorectal squamous cell carcinoma and 22 patients with colonic adenocarcinoma. the cases were further tested by in situ hybridization with biotin-labeled probes specific for human papillomavirus types 6/11, 16/18, and 31/33/35. by polymerase chain reaction, human papillomavirus dna was demonstrated in all of the case ... | 1992 | 1317698 |
| detection of high risk human papillomavirus in routine cervical smears: strategy for screening. | to develop a methodology for direct detection of high risk human papillomavirus (hpv) infection in routine cervical smears by non-isotopic in situ hybridisation (nish) which can be compared with cytopathological assessment of the same cells. | 1992 | 1317884 |
| human papillomavirus (hpv) infection, hiv infection and cervical cancer in tanzania, east africa. | the presence of hpv-dna was determined in tumor biopsies of cervical-cancer patients and in cervical swabs of non-cancer patients from tanzania, east africa, by southern blot hybridization and/or pcr. hpv types 16 and 18 were detected in 38% and 32%, respectively, of 50 cervical-carcinoma biopsies. a consensus primer pcr capable of detecting a broad spectrum of hpv types revealed the presence of hpv-dna in 59% of 359 cervical swabs of non-cancer patients. type-specific pcr showed that types 16 a ... | 1992 | 1318265 |
| human papillomavirus infection and anal carcinoma. retrospective analysis by in situ hybridization and the polymerase chain reaction. | to examine the association of human papillomavirus (hpv) infection with anal squamous cell carcinoma, the authors applied the highly sensitive polymerase chain reaction (pcr) and in situ hybridization (ish) techniques to detect hpv dna in formalin-fixed, paraffin-embedded tissues from 18 patients. the presence of hpv types 16/18 in 3 (16.7%) of 18 patients with anal carcinoma was found, using a colorimetric ish technique for hpv types 6, 11, 16, 18, 31, 35, and 51. results from one of these thre ... | 1992 | 1318640 |
| hpv 16 dna in autopsy material of a metastatic cervical carcinoma. | human papillomavirus (hpv) dna has been regularly detected in primary cervical carcinomas and in some metastatic lesions. using southern blot hybridization on autopsy material we found hpv 16 dna in a primary cervical carcinoma and in multiple metastases therefrom. | 1992 | 1318700 |
| human papillomavirus-associated anogenital neoplasia in persons with hiv infection. | | 1992 | 1318737 |
| early promoters of genital and cutaneous human papillomaviruses are differentially regulated by the bovine papillomavirus type 1 e2 gene product. | the physical state of the human papillomavirus (hpv) genome is usually different in malignant lesions of the skin, in which it is generally found in episomal form, and genital mucosa, in which it is frequently integrated with disruption of the e2 gene. using chimeric or natural hpv promoters in the presence of the bovine papillomavirus type 1 e2 gene product, we observed transcription activation or repression, depending on the distance of e2-binding motifs from the start site. we found a clear d ... | 1992 | 1318941 |
| trichomonas vaginalis (tv) and human papillomavirus (hpv) infection and the incidence of cervical intraepithelial neoplasia (cin) grade iii. | the temporal relationship between cervical infection with trichomonas vaginalis (tv) or human papillomavirus (hpv) and the incidence rate of cervical intraepithelial neoplasia grade three (cin iii) was examined in a cohort of 43,016 norwegian women. from 1980 to 1989, a cervico-vaginal infection from tv and hpv was diagnosed cytologically in 988 and 678 women, respectively. during the 181,240 person-years of observation, 440 cases of cin iii/cervical cancer developed. the age-adjusted incidence ... | 1992 | 1319218 |
| human papillomavirus and herpes virus dna are not detected in benign and malignant prostatic tissue using the polymerase chain reaction. | fresh prostatic tissue removed at the time of surgery was assayed for the presence of human papillomavirus (hpv) types 6, 11, 16, 18 and 33 and herpes and varicella-zoster viruses (hv) using dna amplification followed by specific hybridization. thirty samples representing both benign and malignant prostatic disease were assayed. although appropriate amplimers were present for beta globulin gene indicating successful extraction of dna, no hpv or hv amplimers could be obtained with appropriate pri ... | 1992 | 1319507 |
| [virus and cancer]. | there are at least four viruses tightly associated with human cancer: htly-i and htly-ii with certain leukemias, ebv with lymphomas, bhv with hepatocarcinomas and hpv with genital cancer. in this work we discuss some evidences indicating these associations; in particular we emphasize the characteristics of human papillomavirus (hpv), due to its growing importance in the development of uterine-cervix carcinoma and the mortality of mexican women. the low percentage of infected individuals that dev ... | 1992 | 1319613 |
| detection of human papillomavirus type 16 dna sequences in archival cervical tissues by the polymerase chain reaction. | we have evaluated the polymerase chain reaction for the detection of viral dna sequences in paraffin-embedded archival tissues. in 63 frozen cervical biopsy specimens that were taken from premalignant and invasive lesions, southern blotting detected human papillomavirus (hpv) type 16 dna in 28 (44%) of the samples. in the polymerase chain reaction analysis of the formalin-fixed, paraffin-embedded mirror biopsy specimens, 46 (73%) of the tissues were found to be positive for hpv type 16. in three ... | 1992 | 1319702 |
| detection of human papillomavirus dna sequences in cervical lesions by in situ hybridization using biotinylated dna probes. | ninety-six biopsy specimens were collected for studying the association of specific sequences of hpv dna with genital warts, precancerous lesions and invasive cervical cancer. using dna probes labeled with biotin, 17 of the 22 (77.27%) specimens showing condyloma and 1 of the 14 (7.14%) specimens displaying subclinical papillomavirus infection (spi) of cervix were found to be positive for hpv 6/11; 4 of 23 (17.39%) specimens of squamous epithelial cervical cancer and 2 of the 23 (8.70%) specimen ... | 1992 | 1319885 |
| histological human papillomavirus induced lesions: typization by molecular hybridization techniques. | the viral typization in human papillomavirus (hpv) infections of the lower female genital tract is relevant both from the epidemiological and the clinical point of view. we have tested dna from specimens obtained by guided biopsies on neoplastic and benign lesions, using single probes of the different virus types (6, 11, 16 and 18) by dot-blot and southern blot analysis. according to previous studies hpv 16 and 18 have been detected in neoplastic lesions, while 6 and 11 were more frequently foun ... | 1992 | 1319902 |
| prevalence of human papillomavirus infections among heterosexual men and women with multiple sexual partners. | a prospective study of 65 men and 111 women with multiple heterosexual partners was designed to assess the prevalence and potential risk factors of genital human papillomavirus (hpv) infections. in addition, the hpv reservoir in genital, rectal, and oral mucosa was examined. the specimens for the detection of hpv dna were taken from different sites such as the urethra and coronal sulcus (men), cervix and labia minora (women), anus, rectum, tongue, and buccal mucosa (both men and women). women un ... | 1992 | 1320094 |
| abnormal papanicolaou smears. comparison of cytology, colposcopy and cervical swab dna hybridization. | cervical swab sampling for dna hybridization was performed on 89 women attending a colposcopy clinic. in situ hybridization studies for human papillomavirus (hpv) types 6/11, 16/18 and 31/33/35 were performed on the specimens. biopsy specimens were obtained from 65 patients. the cytologic, colposcopic and histologic results were classified into five groups and assigned a score of 0-5, depending upon the diagnosis. an increasing score correlated positively with the presence of hpv, especially typ ... | 1992 | 1320120 |
| genital human papillomavirus infection. | | 1992 | 1320240 |
| etiology of breast carcinoma: no apparent role for papillomavirus types 6/11/16/18. | a recent study has shown that human papillomavirus (hpv) types 16 and 18 can immortalize normal breast epithelium, and raised the possibility that hpv may be etiologically related to some cases of breast cancer. in order to investigate this possibility, we performed polymerase chain reaction (pcr) assays for hpv types 6, 11, 16 and 18 in 15 papillomas, 15 papillary carcinomas, and 13 infiltrating ductal carcinomas of the breast. no hpv-related dna sequences were identified by southern blotting o ... | 1992 | 1320761 |
| detection of multiple types of human papillomavirus in a giant condyloma from a grafted patient. analysis by immunohistochemistry, in situ hybridisation, southern blot and polymerase chain reaction. | immunosuppressed patients such as transplant recipients are known to develop multiple lesions suggestive of human papillomavirus (hpv) infection. a giant anal condyloma was obtained from a transplant patient; several fragments taken from different areas were examined for the presence of hpv dna using in situ hybridisation, polymerase chain reaction (pcr) and southern blot. typical koilocytes were seen in routinely stained tissue sections, suggesting an hpv infection; furthermore, group specific ... | 1992 | 1320793 |
| general primer polymerase chain reaction in combination with sequence analysis for identification of potentially novel human papillomavirus genotypes in cervical lesions. | we recently described the detection of potentially novel human papillomaviruses (hpv) genotypes (hpv types x [hpv x]) in cervical smears (a. j. c. van den brule, c. j. l. m. meijer, v. bakels, p. kenemans, and j. m. m. walboomers, j. clin. microbiol. 28:2739-2743, 1990) by using the general primer-mediated polymerase chain reaction method (gp-pcr). in this study, the hpv specificities of gp-pcr products were determined by sequence analyses. m13 bacteriophage clones of pcr products derived from c ... | 1992 | 1321168 |
| geographical dependence of sequence variation in the e7 gene of human papillomavirus type 16. | we have determined nucleotide sequences of the e7 open reading frame (orf) of human papillomavirus type 16 (hpv-16) isolates obtained from 32 genital tumours and two hpv-16-transformed human keratinocyte cell lines. in comparison to the prototype hpv-16 isolated from a german cervical cancer biopsy, no sequence variations were noticed in either the two cell lines or the 10 biopsies that were obtained from german patients. in contrast only three of 22 (13.6%) of tanzanian isolates showed the prot ... | 1992 | 1321216 |
| functional interaction of p53 with hpv18 e6, c-myc and h-ras in 3t3 cells. | wild-type (wt) p53 has been suggested to be the product of a tumor-suppressor gene. recently, it has been shown that the e6 oncoproteins of human papillomavirus (hpv) types 16 and 18, like the sv40 large t antigen, are physically associated with wt p53. we have investigated the functional interaction of wt p53 with the viral oncogene products of hpv16 and 18 and with cellular oncogenes by transfection of nih3t3 cells with p53 wt alone or with several oncogene(s). we found that over-expression of ... | 1992 | 1321402 |
| the prevalence of hpv16dna in normal and pathological cervical scrapes using the polymerase chain reaction. | human papillomavirus (hpv) has been implicated in the development of cervical cancer. newly developed molecular techniques have facilitated hpv identification and typing of cervical lesions. several studies have suggested a strong correlation between hpv16 and intraepithelial cervical neoplasia. however, there is no agreement on this point since hpv16 is detected also in normal cervical tissue. we have amplified the l1 region of hpv16 dna using the polymerase chain reaction (pcr) technique and a ... | 1992 | 1321780 |
| retinoblastoma protein binding properties are dependent on 4 cysteine residues in the protein binding pocket. | the retinoblastoma gene product (prb) participates in regulating mammalian cell replication. the mechanism responsible for prb's growth regulatory activity is uncertain. however, prb is known to bind viral transforming proteins including the papilloma virus e7 protein, cellular proteins, and dna. prb contains a critical domain termed the "binding pocket" which is required for binding activities. this binding pocket contains 8 cysteine residues. a naturally occurring mutation affecting one of the ... | 1992 | 1321820 |
| the status of the p53 gene in human papilloma virus positive or negative cervical carcinoma cell lines. | we have analyzed p53 gene alterations in five cervical cancer derived cell lines. two of the five cervical cancer cell lines, htb31 (c-33a) and 32 (ht-3), harbored missense mutations in codons 273 and 245 respectively, whereas the other three tumor cell lines, htb33 (me180), 34 (ms751) and 35 (siha), did not reveal any mutation in the p53 coding sequence spanning codons 126-307. although all the tumor cell lines express comparable levels of p53 rna, only htb31 and htb32 contain high or detectabl ... | 1992 | 1322252 |
| immortalization of fetal mouse brain glial cells by human papillomavirus type 16 e7 genes. | fetal mouse brain glial cells in primary cultures were immortalized by recombinant retroviruses containing human papillomavirus type 16 e7 genes, and named vr-2g cells. the presence and expression of e7 genes in vr-2g cells were demonstrated by the southern and the northern blot analyses. vr-2g cells did not form colonies in soft agar culture, indicating that vr-2g cells have no transforming phenotypes. by the karyotype analysis, vr-2g cells consisted of two cell populations, the pseudo-diploid ... | 1992 | 1322802 |
| human papillomavirus (hpv) type 16 and 33 e6/e7 region transcripts in tonsillar carcinomas can originate from integrated and episomal hpv dna. | this study was undertaken to determine whether human papillomavirus (hpv) e6/e7 gene transcription in tonsillar carcinomas is correlated with viral dna integration. therefore, tonsillar carcinomas containing hpv-16 (n = 2) and hpv-33 (n = 2) dna were analysed for the viral physical state and transcription of the e6/e7 region. southern blot analysis, dna polymerase chain reaction (pcr) and, eventually, two-dimensional gel electrophoresis revealed indications for the presence of only episomal dna ... | 1992 | 1322962 |
| [value of the in situ hybridization technique in the diagnosis of human papilloma virus infections of the uterine cervix. correlations between the human papilloma virus type and the morphological features]. | clinico-pathologic, epidemiologic and molecular analytic studies have shown that: i) the squamous cancer of the uterine cervix derives from intraepithelial lesions with different degrees of atypia; ii) human papillomavirus (hpv) can play a fundamental role in the progression of these lesions. we have examined 219 uterine cervix biopsies by means of the in situ hybridization technique (ish) using biotinylated probes for 6/11, 16/18 and 31/35/51 hpv strains. the histologic lesions were as follows: ... | 1992 | 1323097 |
| correspondence re: r. a. wolber and p. b. clement, in situ dna hybridization of cervical small cell carcinoma and adenocarcinoma using biotin-labeled human papillomavirus probes. mod pathol 4:96, 1991. | | 1992 | 1323114 |
| oral condyloma lesions in patients with extensive genital human papillomavirus infection. | the incidence, location, and morphologic appearances of human papillomavirus oral lesions in patients with genital condylomatosis were investigated with clinical, colposcopic, and histologic examination as diagnostic procedures. the human papillomavirus types were also evaluated with filter in situ hybridization. | 1992 | 1323211 |
| conserved cysteine residue in the dna-binding domain of the bovine papillomavirus type 1 e2 protein confers redox regulation of the dna-binding activity in vitro. | the bovine papillomavirus type 1 e2 open reading frame encodes three proteins involved in viral dna replication and transcriptional regulation. these polypeptides share a carboxyl-terminal domain with a specific dna-binding activity; through this domain the e2 polypeptides form dimers. in this study, we demonstrate the inhibition of e2 dna binding in vitro by reagents that oxidize or otherwise chemically modify the free sulfhydryl groups of reactive cysteine residues. however, these reagents had ... | 1992 | 1323841 |
| human papillomavirus infections in the respiratory tract. | | 1992 | 1323936 |
| expression of transforming growth factor alpha mrna in benign and malignant tissues derived from gynecologic patients with various proliferative conditions. | growth factors are polypeptides involved in the regulation of normal and malignant cell growth. transforming growth factor alpha (tgf alpha) is one of such protein growth factors that plays an important role in the regulation of mammalian cell growth. in this study, the expression of tgf alpha mrna was studied in tissue specimens obtained at the time of surgery from patients with benign and malignant gynecologic proliferative conditions. to analyze tgf alpha mrna expression we utilized the highl ... | 1992 | 1323947 |
| effect on cancer cells of plasmids that express antisense rna of human papillomavirus type 18. | some human squamous cell carcinomas contain dna of human papillomaviruses (hpv) and express rna from the e6 and e7 genes. we have examined the effect of plasmids that express antisense rna of these genes on the growth of the human cancer cell lines hela, c4-1, and 1483, which contain hpv type 18 dna. as controls, the human cancer cell line 183 and the vero line of monkey kidney cells were used, which do not contain hpv. plasmids were introduced into the cells by electroporation; cells that conta ... | 1992 | 1324791 |
| occurrence of p53 gene deletions and human papilloma virus infection in human head and neck cancer. | little is known regarding the molecular genetic events in head and neck carcinoma. epidemiological evidence suggests that both alcohol and tobacco use are related to the development of these neoplasms, and viral infections have also been postulated to play a role in some tumors. loss of p53 tumor suppressor gene function has been found in many malignancies and can occur through either gene mutation or by interaction with the e6 protein of oncogenic human papilloma viruses (hpv). because the muco ... | 1992 | 1324797 |
| the detection of human papillomaviruses in cervical biopsies by immunohistochemistry and in situ hybridization. | the presence of human papillomavirus (hpv) types 6, 16 and 18 in cervical biopsies can be detected by an immunoperoxidase technique using type-restricted monoclonal antibodies raised against fusion proteins representing the l1 major capsid proteins of these three hpv types. in a retrospective study (n = 54) we have used these antibodies and biotinylated dna probes of hpv 6, 16 and 18 to detect and type hpv in formalin-fixed material from the cervix. the biopsies were classified histologically in ... | 1992 | 1325072 |
| long term follow up of women with borderline cervical smear test results: effects of age and viral infection on progression to high grade dyskaryosis. | to follow up and assess the significance of borderline change in cervical smears. | 1992 | 1325232 |
| human papillomavirus identified by nucleic acid hybridization in concomitant nasal and genital papillomas. | presence of human papillomavirus (hpv) as the etiologic agent in nearly all upper respiratory tract recurrent papillomas is well-established. the technique of nucleic acid hybridization now allows specific typing of hpv with a high degree of accuracy. this article reports a series of nine consecutive patients treated for nasal papillomas over the past 9 years. eight of these patients had a personal history of genital papillomas (seven patients) or exposure (one patient). with the use of in situ ... | 1992 | 1325584 |
| specific genetic analysis of microscopic tissue after selective ultraviolet radiation fractionation and the polymerase chain reaction. | a method using selective ultraviolet radiation fractionation followed by polymerase chain reaction (pcr) can analyze specific cell subsets present on a microscope section. direct ultraviolet radiation of fixed and stained tissue sections prevents subsequent amplification by pcr. an "umbrella" or dot placed physically over small numbers of pure cell populations selected by microscopic examination protects these cells from the ultraviolet inactivation. the dna in these protected cells can be speci ... | 1992 | 1325739 |
| clinical course of cervical human papillomavirus lesions in relation to coexistent cervical infections. | a prospective follow-up of 530 women with cervical human papillomavirus (hpv) infection was conducted from 1981 to the present (mean 62.9 months). the patients were examined by pap smears and colposcopy with or without biopsies every sixth month. endocervical swabs were taken for culture of cytomegalovirus (cmv), herpes simplex virus (hsv), and chlamydia trachomatis at each visit. during the follow-up period, 179 of the 530 patients (33.8%) had cervical infection and 351 (66.2%) had no coexisten ... | 1992 | 1326128 |
| analysis of fine-needle aspirates for hpv by pcr may be useful in diagnosis of metastatic gynecologic malignancies. | human papillomavirus (hpv) dna has been shown by molecular hybridization studies to persist in both recurrent and metastatic disease in tumors of the female genital tract. we report here the use of the polymerase chain reaction to identify hpv dna in material from fine-needle aspirates (fna) of recurrent or metastatic lesions to document the primary malignancy arising in the lower genital tract. fine-needle aspirates of suspected recurrent or metastatic tumors were obtained from nine patients wi ... | 1992 | 1326475 |
| detection of antibodies to the e4 or e7 proteins of human papillomaviruses (hpv) in human sera by western blot analysis: type-specific reaction of anti-hpv 16 antibodies. | to determine the cross-reactivity between early (e) proteins of different human papillomavirus (hpv) types, 346 serum samples were tested with e4 and e7 of hpv 16. two hundred and sixteen of them were also tested with hpv 1 e4, 21 with hpv 11 e4 and e7, and 109 with hpv 18 e4 and e7. viral fusion proteins were expressed in escherichia coli and used as antigens in western blot experiments. the sera were obtained from patients with hpv-associated genital lesions or cervical cancer, from renal tran ... | 1992 | 1326715 |
| dot blot hybridization in detection of human papillomavirus (hpv) infections in the oral cavity of women with genital hpv infections. | the presence of human papillomavirus (hpv) types 2, 6, 7, 11, 13 and 16 dna in cytologic scrapings of oral mucosa was studied in 309 women with genital hpv infections. the objective was to test the usefulness of oral mucosal scrapings (3 sequential swabs) in hpv dna detection by dot blot hybridization. based on hybridization with the 32p-labelled alu-repeat probe, most samples contained more than 10(5) cells, which is an adequate number of cells for dot blot hybridization. hybridization with 32p ... | 1992 | 1326738 |
| clinical relevance and evaluation of genitoanal papilloma virus infection in the male. | in the light of current epidemiological data, demonstrating a high prevalence of genitoanal papilloma virus infection (gpvi) existing merely in a subclinical or latent form, ambiguity has emanated on the level of ambition that should be considered optimal for the management of gpvi in the male. this review addresses a pragmatic approach to the problem, with an emphasis on diagnosing and treating overt condylomas causing psychosexual disturbance because of the growth of disfiguring but medically ... | 1992 | 1327056 |
| [uterine cervical carcinoma and human papillomaviruses]. | for many years it has been thought that a significant proportion of cervical cancer could be attributed to sexually transmitted agents, such as sperm, smegma, treponema pallidum, gonococcus and herpes simplexvirus type 2. recent advances of molecular biology, however, have revealed that human papillomavirus (hpv) might be the most causative virus of the disease. since hpv type 16 dna was found in a patient with cervical cancer in 1983, many hpv types have been cloned from cervical cancers, also ... | 1992 | 1327090 |
| human papillomavirus. | in the past year, new data have been published on the molecular biology of human papillomavirus infections and their relationship to cervical neoplasia. as molecular techniques have become more sophisticated and as the molecular knowledge of human papilloma-virus infections has been pursued in greater depth, it is increasingly apparent that this human tumor dna virus is similar to a number of other oncogenic dna viruses that have been described and well studied. these viruses appear to act throu ... | 1992 | 1327250 |
| properties of p53 mutations detected in primary and secondary cervical cancers suggest mechanisms of metastasis and involvement of environmental carcinogens. | primary human papillomavirus (hpv) positive anogenital cancers normally develop without somatic mutation within the p53 gene. in this study, however, we have identified p53 point mutations in metastases arising from hpv positive cervical carcinomas, suggesting that acquisition of p53 mutation may play a role in the progression of some hpv associated primary cancers. p53 mutants identified in anogenital cancers exhibit a dominant transforming phenotype and increased resistance to hpv16 e6 directe ... | 1992 | 1327751 |
| the predictive value of human papilloma virus (hpv) typing in the prognosis of bronchial squamous cell papillomas. | five solitary squamous papillomas of bronchus with variable degrees of dysplasia, one combined with a laryngeal papilloma and with a neuroendocrine carcinoma in the contralateral lung, and five papillomas combined with invasive squamous cell carcinomas were investigated for their expression of human papilloma virus dna by in situ hybridization. benign squamous cell papillomas showed an association with papilloma virus type 11 and rarely type 6, whereas types 16 or 18, sometimes in combination wi ... | 1992 | 1328017 |
| epidermal growth factor receptor expression and the presence of human papillomavirus in cervical squamous intraepithelial lesions. | to determine the relationship between the expression of epidermal growth factor receptor (egfr) and the presence or type of human papillomavirus (hpv) in cervical squamous intraepithelial lesions (sil), paired colposcopically directed cervical biopsies were obtained from 88 patients referred for abnormal papanicolaou smears. one biopsy was formalin-fixed and processed for conventional light microscopy, and the other was immediately frozen. a portion of the frozen tissue was used for southern blo ... | 1992 | 1328080 |
| human papillomavirus and its prognostic significance in invasive carcinoma of the cervix in young patients. | of 1,200 chinese patients treated for carcinoma of the cervix between 1975 and 1984, those aged 40 years (n = 70) or less had a poorer prognosis. of the latter group, 71% had disease classified as less than international federation of gynecology and obstetrics (figo) stage ib, and in 91% the disease was less than stage iia; however, the 5-year survival of the 45 patients with verified invasive carcinoma was 75% compared with 82% for the overall group. the polymerase chain reaction (pcr) was used ... | 1992 | 1328083 |
| polymerase chain reaction detection of human papillomavirus: quantitation may improve clinical utility. | a case-control study compared detection by polymerase chain reaction (pcr) specific for human papillomavirus (hpv) type 16 with restriction enzyme analysis and southern blot hybridization detection of hpv type 16. cervicovaginal lavage samples from 64 women with histopathologic evidence of a cervical squamous intraepithelial lesion and 55 samples from cytologically healthy women were studied. several methods of pcr product analysis, including radioactive and nonradioactive probing, were compared ... | 1992 | 1328278 |
| [detection of human papillomavirus dna using in situ hybridization]. | | 1992 | 1328713 |