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rapid and sensitive detection of campylobacter spp. in chicken products by using the polymerase chain reaction.the polymerase chain reaction (pcr) after a short enrichment culture was used to detect campylobacter spp. in chicken products. after the 16s rrna gene sequence of campylobacter jejuni was determined and compared with known sequences from other enterobacteria, a primer and probe combination was selected from the region before v3 and the variable regions v3 and v5. with this primer set and probe, 426-bp fragments from c. jejuni, campylobacter coli, and campylobacter lari could be amplified. the d ...19921282312
prevalence, biotypes, plasmid profile and antimicrobial resistance of campylobacter isolated from wild and domestic animals from northeast portugal.the incidence of campylobacter jejuni and campylobacter coli in wild and producing animals has been studied to evaluate their importance as potential reservoirs of campylobacter infection. these organisms were isolated from: 59 chicken (60.2%), 65 swine (59.1%), 31 black rats (57.4%), 61 sparrows (45.5%), 21 ducks (40.5%), 32 cows (19.5%) and 27 sheep (15.3%). biotypes, plasmid and resistance profiles were studied in order to characterize the isolates. biotypes i and ii of c. jejuni were predomi ...19921429305
potential sources of campylobacter species in the homes of farmworkers in zimbabwe.chicken faeces, houseflies, household stored drinking water, cooked or prepared foods and handwashings of people of all age groups and hands of children under 5 years old were examined as potential sources and transmission routes of campylobacter species in a farmworker community. campylobacter species were found to be common in chicken faeces collected from the homesteads of the farmworkers but were rarely isolated from houseflies or household stored drinking water. they were not isolated from ...19911758009
raw beef, pork and chicken in japan contaminated with salmonella sp., campylobacter sp., yersinia enterocolitica, and clostridium perfringens--a comparative study.one hundred and twenty samples each of raw ground beef, pork and chicken from ten local grocery stores in shimane prefecture, japan, were examined for the presence of salmonella sp. (sal), campylobacter jejuni (cj), campylobacter coli (cc), yersinia enterocolitica (ye), and clostridium perfringens (cp) from april 1984 to march 1985. a total of 205 isolates of sal (112 strains), cj (64 strains), cc (one strain), ye (7 strains) and cp (21 strains) were recovered from 17 beef (14.2%), 31 pork (25.8 ...19872887078
a note on adhesion of bacteria to chicken muscle connective tissue.laboratory cultures of bacteria were tested for the ability to attach to collagen fibres of intact chicken muscle connective tissue. all salmonellas, fimbriate strains of escherichia coli and a strain of campylobacter coli were able to attach to tissue only when suspended in distilled water. prior immersion of tissue in sterile water for 20 min or extended immersion in these bacterial suspensions was a prerequisite for adhesion. attachment could be prevented by the addition of physiological leve ...19872888744
[survey for the contamination of campylobacter jejuni and campylobacter coli among raw meat and the environment of poultry processing plants]. 19883137280
serovars and biovars of campylobacter strains isolated from humans and slaughterhouse animals in northern germany.a total of 318 campylobacter strains from sporadic cases of human enteritis (109 strains) and healthy slaughterhouse animals in northern germany (209 strains) were bio- and serotyped according to the lior typing schemes. three hundred strains were typable (94.3%) and 38 serovars were identified. among human strains 28 serovars were identified with 30% of them belonging to serovar 4. strains from pigs were associated with 25 serovars, the most frequent being serovar 20 (21.2%). fourteen serovars ...19883372398
[experimental animal studies with campylobacter jejuni and campylobacter coli in chickens]. 19873566434
[the epidemiology of campylobacter jejuni and campylobacter coli in the fattening chicken]. 19873589638
campylobacter colitis in ranch mink in ontario.outbreaks of colitis, where campylobacter jejuni and campylobacter coli were the only pathogens isolated occurred in weanling mink (mustella vision) on two commercial mink ranches in ontario. lesions were restricted to the proximal colon and were characterized by multiple 1 mm focal or 1 mm linear erosions/ulcers in the region 2 cm distal to the ileal-colonic junction. histological changes included thickening of the colonic mucosa, inflammatory cell infiltrate in the lamina propria and submucosa ...19863742356
the chicken embryo as a model for campylobacter invasion: comparative virulence of human isolates of campylobacter jejuni and campylobacter coli.eleven-day-old chicken embryos were used to compare the relative virulence of minimally passaged human isolates of campylobacter jejuni and campylobacter coli. graded doses of bacteria were inoculated onto the chorioallantoic membrane, and 50% lethal doses were calculated at 72 h postinfection. strains varied markedly in their ability to invade the chorioallantoic membrane and kill the embryos. the 50% lethal doses varied by about 6 logs for 25 strains of c. jejuni, and by 2 logs for 5 strains o ...19863759232
[isolation of campylobacter jejuni and campylobacter coli from intestinal contents and feces of chickens]. 19853925041
serotyping of campylobacter jejuni, campylobacter coli, and campylobacter laridis from domestic and wild animals.by using 50 unabsorbed antisera, we were able to serotype 272 (65.7%) of 414 thermotolerant campylobacters from wild and domestic animals, on the basis of heat-stable antigens identified by means of passive hemagglutination. forty-two serotypes were recognized. the pattern of serotypes detected in the various animal species was compared to human clinical isolates by using the czekanowski index (proportional similarity index). the highest degree of similarity to the clinical isolates was observed ...19854015088
biotypes and serotypes of campylobacter jejuni and campylobacter coli strains isolated from patients, pigs and chickens in the region of rotterdam.a comparison was made of the distribution of the bio-and serotypes of campylobacter jejuni and campylobacter coli isolated from human patients (205 isolates), intestinal contents of pigs (163 isolates) and intestinal contents and livers of chickens (147 isolates). all strains were isolated in the region of rotterdam. campylobacter jejuni biotype 1 accounted for 86%, 1% and 82% of the human, pig and chicken isolates respectively; c. coli was present in 7%, 98% and 14% respectively. serotyping was ...19854031520
occurrence of plasmids and antibiotic resistance among campylobacter jejuni and campylobacter coli isolated from healthy and diarrheic animals.serologically defined strains of campylobacter jejuni and campylobacter coli from healthy and diarrheic animals were examined for the occurrence of plasmid dna in association with the antibiotic susceptibility of the bacterial host and the health status of the animal host. of all campylobacter organisms surveyed, 53% (116 of 200) contained plasmid dna. a plasmid occurrence rate of 73.8% was obtained for c. coli from healthy pigs, contrasted by lower plasmid occurrence rates for c. coli from diar ...19854044793
identification of campylobacter coli isolates from animals and humans by bacterial restriction endonuclease dna analysis.ninety-nine campylobacter coli isolates were examined by bacterial restriction endonuclease dna analysis (brenda) with hindiii. isolates from poultry from the same environment had identical patterns, patterns of isolates carried by suckling piglets were generally the same as those of isolates recovered from their dams, and one human patient yielded the same brenda type when sampled 6 weeks later. the 14 human isolates examined produced 11 distinct brenda types. forty-three c. coli isolates from ...19846093690
campylobacter jejuni and campylobacter coli serotypes isolated from chickens, cattle, and pigs.a total of 191 campylobacter jejuni and 125 campylobacter coli were isolated from the intestinal content of 398 chickens, 421 cattle, and 203 pigs. all 108 chicken isolates and 73 of 80 cattle isolates were c. jejuni, but 115 of the 118 pig isolates were c. coli. a total of 84% of the c. jejuni and 64% of the c. coli isolates were typed on the basis of thermostable antigens with 20 antisera prepared against frequently occurring serotypes in campylobacter enteritis in man (15 c. jejuni, 6 c. coli ...19836630466
pathogenicity of campylobacter jejuni and campylobacter coli strains in the pregnant guinea pig model.pathogenicity of 17 campylobacter isolates for pregnant guinea pigs was investigated. of 14 isolates, 12 (86%) produced rates of abortion ranging from 13% to 87%. two isolates did not produce abortion. reference strains of c fetus subsp venerealis produced abortion in 60% to 87% and c fetus subsp fetus produced abortion in 60% of the guinea pigs. inoculated organisms were recovered from uterus, blood, liver, kidney, spleen, and gallbladder of the guinea pigs at rates as high as 83% for 2 ovine i ...19836650962
house flies (musca domestica) as possible vectors of campylobacter fetus subsp. jejuni.a total of 161 strains of campylobacter fetus subsp. jejuni were isolated from house flies (musca domestica). the carrier rates detected were 50.7% in flies captured on a chicken farm and 43.2% in flies from a piggery. the relative prevalences of campylobacter coli, c. jejuni, and nalidixic acid-resistant thermophilic campylobacters were 90.1, 6.2, and 3.7%, respectively. the results indicate that flies may play a linking role in the epidemiology of campylobacter infection in humans by transmitt ...19836830213
recovery of campylobacter jejuni and campylobacter coli from inoculated foods by selective enrichment.a direct enrichment procedure was developed to selectively recover small numbers of campylobacter jejuni, c. coli, and nalidixic acid-resistant thermophilic campylobacter from foods. the procedure includes an enrichment medium composed of brucella broth, 7% lysed horse blood, 0.3% sodium succinate, 0.01% cysteine hydrochloride, vancomycin (15 micrograms/ml), trimethoprim (5 micrograms/ml), polymyxin b (20 iu/ml), and cycloheximide (50 micrograms/ml) that is inoculated with 10 or 25 g of food and ...19827103488
pathogenicity of campylobacter jejuni isolates from animals and humans.fourteen isolates of campylobacter jejuni of different serotypes and one campylobacter coli isolate, from various human and animal sources, were tested for potential pathogenic mechanisms. enterotoxin production was not detected in the infant mouse test or by calf and piglet ligated intestinal loop studies. isolates were not invasive by the sereny test. all isolates associated with and penetrated hela cells, although both actions occurred generally in a minor way under the conditions of our stud ...19827141696
diffusion of thermophilic campylobacter in the pesaro-urbino area (italy) from 1985 to 1992.the results of research on the spreading of campylobacter in the pesaro-urbino area carried out from 1985 to 1992 are presented. materials of different origin were examined: 822 samples of human faeces, 533 animal rectal swabs, 192 samples of domestic sewage, 48 of river water, 96 of sea water and 632 of various types of food. two hundred and nine strains of campylobacter were isolated (9%), most of which were campylobacter jejuni (80%), with particular frequency in food products (chicken carcas ...19957489778
high-resolution genotyping of campylobacter coli identifies clones of epidemiologic and evolutionary significance.campylobacter coli strains from clinical and other sources were examined in terms of o (heat-stabile; hs) serotype and by several molecular typing techniques. restriction fragment length polymorphism (rflp) around the three 16s rrna genes revealed 10 variants, none found in campylobacter jejuni. rflp analysis of a polymerase chain reaction amplicon generated from the flagellin gene (flaa) yielded 11 polymorphism groups, some of them linked to hs serotypes. enlarged flaa genes, contributing three ...19957561196
effect of the complement system on the sensitivity of campylobacter jejuni and campylobacter coli to human blood serum.we determined the sensitivity of five strains of campylobacter jejuni and c. coli isolated from children with diarrhea and from chicken feces to normal human blood serum (undiluted and at concentrations of 10, 30, 50 and 70%), a hypogammaglobulinemic serum and a complement-deficient serum. both species were highly sensitive to the bactericidal activity of human serum, regardless of their source. the highest bactericidal activity was observed with pooled fresh normal serum, with bacterial surviva ...19957581046
differentiation of campylobacter jejuni and campylobacter coli strains by using restriction endonuclease dna profiles and dna fragment polymorphisms.the chromosomal dna fragment patterns from a total of 169 campylobacter jejuni and campylobacter coli isolates from poultry and humans were analyzed by using dna restriction endonucleases clai and ecorv. the dna restriction patterns produced by clai and ecorv consisted of unique dna fragments of 9 to 9.5 kb and 3.5 kb generated with clai and a single unique fragment of 3.0 kb produced by ecorv. these patterns were obtained with all strains of c. jejuni tested. the dna restriction patterns were f ...19957615717
[infectivity of campylobacter jejuni and campylobacter coli in chickens].the infectivity of four campylobacter (c.) jejuni strains and one c. coli strain was evaluated by the reisolation rate from cloacal swabs after oral infection of chickens at four weeks of age. one strain did not colonize the digestive tract, another infected only a part of the experimental group and a third was excreted at a lower percentage in the first two weeks p.i. compared to the other two strains. in correlation to age, the excretion rate was highest in 7-week-old birds. in groups of 4 and ...19957646423
occurrence of plasmids and tetracycline resistance among campylobacter jejuni and campylobacter coli isolated from whole market chickens and clinical samples.twenty whole market chickens, purchased from 10 different stores in the taipei metropolitan area, were examined for the presence of campylobacter jejuni and campylobacter coli. the microorganisms were recovered from 95% of the chickens. a survey of different sites on--breast, thigh and tail--showed that contamination was equally common on all these sites. one hundred and sixty-seven chicken isolates and the 41 clinical isolates of campylobacter jejuni were examined for the occurrence of plasmid ...19947703010
restriction fragment length polymorphisms among the flagellar genes of the lior heat-labile serogroup reference strains and field strains of campylobacter jejuni and c. coli.several typing systems have been described for campylobacter jejuni and c. coli, to assess the complex epidemiology of these important enteric pathogens. in the present study two typing methods, slide agglutination according to the lior scheme, and the demonstration of restriction-fragment length polymorphisms (rflp) of flagellar genes, have been used in parallel on a set of 194 strains. this set comprised 118 sero-reference strains of c. jejuni and c. coli of the lior scheme, as well as 76 clin ...19957781730
prevalence of campylobacter enteritis in children from yaounde (cameroon).a cross sectional study on the prevalence of campylobacter jejuni/coli and its possible aetiologic role in childhood diarrhoea in yaounde was undertaken in 272 children presenting with diarrhoea and 157 age matched controls from april 1989 to october 1990. stool cultures were performed according to standard techniques for campylobacter jejuni/coli shigella and salmonella species. rotavirus was detected using a latex agglutination test. campylobacter jejuni/coli was isolated from 21 (7,7 pc) of 2 ...19957788677
[the fatty acid composition of the total lipids in bacteria of the genus campylobacter].composition of fatty acids of total lipids in home strains of campylobacteria has been studied. lipids of all the strains of c. jejuni and c. coli mainly consist of saturated fatty acids (from 75.7 to 78.7%) with predominance of tetradecanoic and hexadecanoic fatty acids. the level of unsaturated fatty acids is considerably lower (from 21.0 to 22.5%); these acids are mainly presented by hexadecene acid. qualitative composition of fatty acids of total lipids in c. jejuni and c. coli does not perm ...19947952227
efficacy of filter types for detecting campylobacter jejuni and campylobacter coli in environmental water samples by polymerase chain reaction.a previously developed polymerase chain reaction (pcr) amplification of a target region in the flaa campylobacter flagellin gene was evaluated and adapted for use with environmental water samples. the ability to detect campylobacter jejuni or campylobacter coli in seeded water samples was tested with various filters after concentration and freeze-thaw lysis of the bacterial cells. a nonradioactive probe for the amplified flagellin gene fragment detected as little as 1 to 10 fg of genomic dna and ...19938285708
effect of host lineage on the virulence of campylobacter jejuni/coli in the chick embryo model.the chorioallantoic membrane (cam) inoculated chick embryo model was used to study the effect of host lineage on the virulence of campylobacter jejuni and campylobacter coli. ld50 values were used to compare the susceptibilities of chick embryos from eight inbred chicken lines to infection by four strains of c. jejuni and one strain of c. coli. differences in susceptibility were found between inbred chicken lines. these were shown not to be due to maternal antibody status, nor transfer of antibo ...19938454192
the role of management systems in the epidemiology of thermophilic campylobacters among poultry in eastern zone of tanzania.a total of 255 samples of droppings collected from a total of 22 different poultry units were examined for the presence of thermophilic campylobacters and the isolates biotyped using skirrow's protocol. the organisms were isolated from 90 (35.3%) of all samples. among the 22 units investigated, 13 (59%) were found to have unsatisfactory management systems, while 7 (32%) and 2 (9%) were found to have unsatisfactory and good systems respectively. significantly large numbers of isolations, 68 of 14 ...19938472771
ciprofloxacin resistant campylobacter spp. in humans: an epidemiological and laboratory study.from the end of april 1991 until the end of 1991, 2209 isolates of campylobacter spp. have been collected in plymouth phl of which 91 (4.1%) were resistant to ciprofloxacin. none of the 91 patients involved had taken a quinolone, but 30/91 (33%) had travelled abroad (16 to the iberian peninsula) in the three months preceding isolation of the ciprofloxacin-resistant campylobacter spp. in the case-control study 12/15 (80%) of the cases had recently consumed poultry as had 20/24 (83%) of controls w ...19968722540
development of a rapid and specific colony-lift immunoassay for detection and enumeration of campylobacter jejuni, c. coli, and c. lari.contamination of retail poultry by campylobacter spp. is a significant source of human diarrheal disease. we have developed a colony-lift immunoassay (cli) for the detection of campylobacter jejuni, c. coli, and c. lari isolated from such sources and grown on selective agar medium or on filter membranes. this technique has been successfully utilized to quantify campylobacter colonies within 18 to 28 h after sampling. hydrophobic, high-protein-binding membranes were prewet with methanol and used ...19968914757
detection of campylobacter jejuni and camp. coli in chicken faecal samples by pcr.pcr primers were selected from the flagellin gene sequences flaa and flab of campylobacter coli to amplify dna from camp. jejuni and camp. coli. when the pcr products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of camp. jejuni and camp. coli. the assay was used to analyse 31 chicken faecal samples. full correspondence was found between the pcr assay conducted on the enriched cultures and the standard cultu ...19968987720
antimicrobial susceptibility patterns of thermophilic campylobacter spp. from humans, pigs, cattle, and broilers in denmark.the mics of 16 antimicrobial agents were determined for 202 campylobacter jejuni isolates, 123 campylobacter coli isolates, and 6 campylobacter lari isolates from humans and food animals in denmark. the c. jejuni isolates originated from humans (75), broilers (95), cattle (29), and pigs (3); the c. coli isolates originated from humans (7), broilers (17), and pigs (99); and the c. lari isolates originated from broilers (5) and cattle (1). all isolates were susceptible to apramycin, neomycin, and ...19979333055
specific detection and confirmation of campylobacter jejuni by dna hybridization and pcr.conventional detection and confirmation methods for campylobacter jejuni are lengthy and tedious. a rapid hybridization protocol in which a 1,475-bp chromogen-labelled dna probe (pdt1720) and campylobacter strains filtered and grown on 0.22-micron-pore-size hydrophobic grid membrane filters (hgmfs) are used was developed. among the environmental and clinical isolates of c. jejuni, campylobacter coli, campylobacter jejuni subsp. doylei, campylobacter lari, and arcobacter nitrofigilis and a panel ...19979361442
isolation and molecular analysis of colonising and non-colonising strains of campylobacter jejuni and campylobacter coli following experimental infection of young chickens.fourteen-day-old chickens were inoculated with selected campylobacter coli and c. jejuni strains. c. jejuni strains were of two subgroups based on a polymorphism detected using a dna probe and represented the profiles typical for the majority of strains of either chicken or human origin. all c. coli strains previously isolated from humans colonised chickens, whereas from 4/7 c. jejuni strains of human origin, failed to colonise. of 12 campylobacter strains of chicken origin, 10 established a per ...19989646454
identification of the enteropathogens campylobacter jejuni and campylobacter coli based on the cadf virulence gene and its product.campylobacter jejuni and campylobacter coli are common causes of gastroenteritis in humans. infection with c. jejuni or c. coli is commonly acquired by eating undercooked chicken. the goal of this study was to develop specific detection assays for c. jejuni and c. coli isolates based on the cadf virulence gene and its product. the cadf gene from c. jejuni and c. coli encodes a 37-kda outer membrane protein that promotes the binding of these pathogens to intestinal epithelial cells. a fragment of ...19999986804
serotypes and typability of campylobacter jejuni and campylobacter coli isolated from poultry products.campylobacter infection is one of the most common bacterial enteric pathogens. campylobacter jejuni and campylobacter coli infections are mostly food- and waterborne and especially poultry is often assumed to be an important source. the heat-stable serotyping system (the 'penner' scheme) was used to study the serotype distribution of c. jejuni and c. coli isolated from different food products of poultry origin sampled from retail outlets in denmark. a total of 156 isolates were serotyped, 85% of ...199910100900
detection of cytolethal distending toxin activity and cdt genes in campylobacter spp. isolated from chicken carcasses.this study was designed to determine whether isolates from chicken carcasses, the primary source of campylobacter jejuni and campylobacter coli in human infections, commonly carry the cdt genes and also whether active cytolethal distending toxin (cdt) is produced by these isolates. campylobacter spp. were isolated from all 91 fresh chicken carcasses purchased from local supermarkets. campylobacter spp. were identified on the basis of both biochemical and pcr tests. of the 105 isolates, 70 (67%) ...199910103243
differentiation of campylobacter coli and c. jejuni by length and dna sequence of the 16s-23s rrna internal spacer region.the internal spacer region (isr) between the 16s and 23s rrna genes of campylobacter was investigated by pcr fragment length typing and dna sequencing of clinical and chicken wild-type isolates. pcr fragment length typing showed one fragment of 859 nt in length for the 12 strains of campylobacter coli investigated. thirty-six of the campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. three strains showed two fragments between 501 and 92 ...199910206715
study of the presence of campylobacter jejuni and c. coli in sand samples from four swiss chicken farms.chicken farms are frequently infected with campylobacter jejuni and campylobacter coli. the objective of the present study was to investigate environmental samples from chicken farms for the presence of c. jejuni and c. coli. every week between july and november 1997, three sand samples from the runs of four chicken farms were analyzed by culture and directly by polymerase chain reaction (pcr). these two detection methods were compared to each other. a total of 231 samples were tested. eleven sa ...199910229951
biotin-streptavidin enzyme-linked immunosorbent assay for the detection of antibodies to campylobacter jejuni and c. coli in chickens.an enzyme-linked immunosorbent assay (elisa) was developed in a homologous system with bacterial ultrasonic-treated proteins as the antigen and antisera from chickens infected orally and subcutaneously with the strain campylobacter jejuni serovar 6 (cj 6). the cut-off level was determined using antisera from non-infected specific-pathogen-free chickens up to the age of 10 weeks. the suitability of the elisa system was verified using antisera taken from chickens orally infected at the age of 4 we ...199910337238
generation of a superoxide dismutase (sod)-deficient mutant of campylobacter coli: evidence for the significance of sod in campylobacter survival and colonization.the microaerophilic nature of campylobacter species implies an inherent sensitivity towards oxygen and its reduction products, particularly the superoxide anion. the deleterious effects of exposure to superoxide radicals are counteracted by the activity of superoxide dismutase (sod). we have shown previously that campylobacter coli possesses an iron cofactored sod. the sodb gene of c. coli ua585 was insertionally inactivated by the site-specific insertion of a teto cassette. organisms harboring ...199910347040
incidence of salmonella, campylobacter jejuni, campylobacter coli, and listeria monocytogenes in poultry carcasses and different types of poultry products for sale on the belgian retail market.from january 1997 to may 1998, 772 samples of poultry carcasses and poultry products for sale on the retail market in belgium were analyzed for the presence of salmonella spp., salmonella enteritidis, campylobacter jejuni, c. coli, and listeria monocytogenes per 100 cm2 or 25 g. poultry samples were contaminated with salmonella (36.5%), c. jejuni and c. coli (28.5%), and l. monocytogenes (38.2%). in about 12.3% of the poultry samples, the l. monocytogenes contamination level exceeded 1 cfu per g ...199910419264
the absence of cecal colonization of chicks by a mutant of campylobacter jejuni not expressing bacterial fibronectin-binding protein.campylobacter jejuni is a common cause of human gastrointestinal illness throughout the world. infections with c. jejuni and campylobacter coli are frequently acquired by eating undercooked chicken. the ability of c. jejuni to become established in the gastrointestinal tract of chickens is believed to involve binding of the bacterium to the gastrointestinal surface. a 37-kd outer membrane protein, termed cadf, has been described that facilitates the binding of campylobacter to fibronectin. this ...199910494431
flagellin gene polymorphism analysis of campylobacter compared with antigen serotyping.flagellin gene sequence polymorphisms were used to discriminate amongst 53 strains of campylobacter jejuni and c. coli. the campylobacter strains were made up of forty-three strains of campylobacter jejuni and 10 strains of campylobacter coli. the results were analysed in relation to penner serotyping. twenty dna pcr-rflp patterns (genotypes) were identified by analysis of dde i fragment length polymorphisms in flagellin gene (fla a and fla b) polymerase chain reaction (pcr) products. flagellin ...199910627993
detection and differentiation of campylobacter jejuni and campylobacter coli in broiler chicken samples using a pcr/dna probe membrane based colorimetric detection assay.campylobacter enteritis in humans has been linked to consumption of poultry meat. surveys show that 30-100% of poultry harbour campylobacter as normal flora of the digestive tract which indicates a need to identify prevalent organism types in flocks and trace their epidemiology. in this study we describe a campylobacter genus specific polymerase chain reaction (pcr) assay, amplifying the 16 s-23 s rrna intergenic spacer region with an internal campylobacter genus specific dna probe and species s ...200010725058
rapid and specific enzyme immunoassay on hydrophobic grid membrane filter for detection and enumeration of thermophilic campylobacter spp. from milk and chicken rinses.six commercially available anti-campylobacter antibodies were examined for their applicability in an enzyme immunoassay on hydrophobic grid membrane filters, both for the detection and enumeration of campylobacter spp. when a panel of nine campylobacter (seven campylobacter jejuni and two campylobacter coli) and eight non-campylobacter strains were used in a dot-blot format enzyme immunoassay to test the specificity of these antibodies, only one polyclonal antibody (biodesign) detected all campy ...200010772214
genotyping of clinical and chicken isolates of campylobacter jejuni and campylobacter coli.genomic dna from 58 strains of campylobacter made up of 48 campylobacter jejuni and ten campylobacter coli were digested with sma i and analysed by pulsed-field gel electrophoresis (pfge). the cleavage of dna by sma i gave 22 distinct hybridization patterns. the two campylobacter species were subtyped by pfge. the average genomic size for c. jejuni by sma i digestion was 1.73 mb, while that of c. coli gave 1.7 mb. results from this study indicate that pfge analysis by sma i digested genomic dna ...200011077971
campylobacter contamination of raw meat and poultry at retail sale: identification of multiple types and comparison with isolates from human infection.campylobacter species are the major cause of acute bacterial enteritis reported in the united kingdom, nonetheless many aspects of campylobacteriosis epidemiology remain poorly understood. the aim of this study was to determine the prevalence of campylobacter jejuni and campylobacter coli in fresh bovine, ovine, and porcine liver and chicken portions from retail outlets and compare strain subtype distributions with those associated with cases of human campylobacteriosis occurring within the same ...200011131886
detection of thermophilic campylobacter from sparrows by multiplex pcr: the role of sparrows as a source of contamination of broilers with campylobacter.the best combination of primers and the annealing temperature of multiplex pcr for campylobacter jejuni, campylobacter coli, and campylobacter lari were examined. the multiplex pcr was able to detect type strains of the three species. all results of identification of wild strains (30 strains of c. jejuni, 20 strains of c. coli, and 4 strains of c. lari) by the multiplex pcr coincided with those of the conventional biochemical identification tests, suggesting that the multiplex pcr can simultaneo ...200011193345
pcr-elisas for the detection of campylobacter jejuni and campylobacter coli in poultry samples.campylobacter species, primarily campylobacter jejuni and campylobacter coli, are regarded as a major cause of human gastrointestinal disease, commonly acquired by eating undercooked chicken. we describe a pcr-elisa for the detection of campylobacter species and the discrimination of c. jejuni and c. coli in poultry samples. the pcr assay targets the 16s/23s ribosomal rna intergenic spacer region of campylobacter species with dna oligonucleotide probes designed for the specific detection of c. j ...200111252796
serotype and genotype diversity and hatchery transmission of campylobacter jejuni in commercial poultry flocks.we investigated the genotype and serotype diversity of campylobacter coli and c. jejuni in two parent flocks of adult hens and their offspring over two rotations in order to evaluate the role of hatchery mediated transmission and/or vertical transmission of campylobacters in broiler flocks. in total, 314 c. jejuni and 32 c. coli isolates from parent and broiler flocks and from the surroundings of broiler houses were typed by flagellin gene pcr/rflp (fla-typing), and selected isolates were also t ...200111423205
antimicrobial susceptibilities of campylobacter strains isolated from food animals in belgium.campylobacter spp. are a frequent cause of diarrhoea in man, originating mostly from poultry. it has been suggested that the veterinary use of antibiotics is largely responsible for resistance in human isolates, particularly to quinolones. during a 6 month period from june to december 1998, 677 campylobacter isolates were obtained from healthy poultry and pigs. samples were taken at belgian slaughterhouses. species identification was performed by biochemical tests, multiplex pcr and sds-page of ...200111481294
differentiation of campylobacter species by aflp fingerprinting.the fluorescent amplified fragment length polymorphism (aflp) fingerprinting method was tested for its ability to identify and subtype the most important campylobacter species found in veterinary infections. sixty-nine reference strains and 19 clinical isolates of campylobacter jejuni subsp. jejuni, campylobacter jejuni subsp. doylei, campylobacter upsaliensis, campylobacter coli, campylobacter lari, campylobacter fetus subsp. fetus, c. fetus subsp. venerealis, campylobacter hyointestinalis subs ...200111577152
prevalence of campylobacter spp., escherichia coli, and salmonella serovars in retail chicken, turkey, pork, and beef from the greater washington, d.c., area.a total of 825 samples of retail raw meats (chicken, turkey, pork, and beef) were examined for the presence of escherichia coli and salmonella serovars, and 719 of these samples were also tested for campylobacter spp. the samples were randomly obtained from 59 stores of four supermarket chains during 107 sampling visits in the greater washington, d.c., area from june 1999 to july 2000. the majority (70.7%) of chicken samples (n = 184) were contaminated with campylobacter, and a large percentage ...200111722889
isolation and polymerase chain reaction-based detection of campylobacter jejuni and campylobacter coli from poultry in the philippines.the polymerase chain reaction (pcr) and the conventional culture method of detecting thermophilic campylobacter species in duck and chicken samples from two locations in the province of laguna, philippines, were compared. three campylobacter jejuni and five c. coli strains were isolated from a total of 135 duck and chicken samples from both methods. the pcr technique, however, was found to be more sensitive, accurate and rapid than the conventional culture method. the specificity of two sets of ...200111759758
cecal colonization of chicks by porcine strains of campylobacter coli.ten genotypically distinct strains of campylobacter coli were isolated from a swine production facility. these porcine isolates were then orally inoculated into day-of-hatch leghorn chicks and were excellent colonizers of the chick cecum. campylobacter coli recovered from inoculated chickens were genotypically identical to the challenge strain. the absence of host specificity suggests a possible movement of strains among swine, field animals and birds, and poultry houses.200212061661
prevalence of thermophilic campylobacter spp. in ready-to-eat foods and raw poultry in northern ireland.although there have been numerous studies investigating the prevalence of campylobacters in animals and raw meats, there are limited data on the persistence of these organisms in ready-to-eat (rte) foodstuffs. although poultry is now well established as a major reservoir of thermophilic campylobacters, it is widely assumed that hazard analysis critical control point (haccp) controls in commercial and industrial settings are effective in eliminating this hazard through thorough cooking of rte pro ...200212182488
quorum sensing and production of autoinducer-2 in campylobacter spp., escherichia coli o157:h7, and salmonella enterica serovar typhimurium in foods.autoinducer molecules are utilized by gram-negative and gram-positive bacteria to regulate density-dependent gene expression by a mechanism known as quorum sensing. pcr and dna sequencing results showed that campylobacter jejuni and campylobacter coli possessed luxs, which is responsible for autoinducer-2 (ai-2) production. using a vibrio harveyi luminescence assay, the production of ai-2 was observed in milk, chicken broth, and brucella broth by c. coli, c. jejuni, salmonella enterica serovar t ...200212200329
rapid pcr using nested primers of the 16s rrna and the hippuricase (hip o) genes to detect campylobacter jejuni and campylobacter coli in environmental samples.identification of sources campylobacter infection in the poultry houses is in general problematic due to the lack of reliable methods to detect campylobacteria in environmental samples. detection of campylobacteria in environmental samples by conventional culture methods is difficult and of limited sensitivity due to the use of selective media, the low number of bacteria in the samples and possibly also due to the presence of non-culturable or sub-lethally injured stages of the bacteria. the pre ...200212477440
occurrence and resistance to antibiotics of campylobacter jejuni and campylobacter coli in animals and meat in northeastern italy.a study was carried out in northeastern italy during 2000 and 2001 to investigate the occurrence of campylobacter jejuni and campylobacter coli in animals, cattle, pigs, and broilers, and raw meat, beef, pork, and chicken. campylobacter spp. were detected in 53.9% of the cattle, 63.5% of the pigs, and 82.9% of the broilers examined. chicken meat was frequently contaminated (81.3%), while lower rates were found in pork meat (10.3%) and beef (1.3%). the resistance to antibiotics of the strains was ...200312593931
molecular characterization of fluoroquinolone-resistant campylobacter spp. isolated from poultry.campylobacteriosis, an infectious disease caused by campylobacter jejuni and campylobacter coli, is treated by fluoroquinolone antibiotics in clinical practices. however, use of these drugs in animal husbandry may select for fluoroquinolone-resistant campylobacters and, thereby, compromise the clinical treatment of infection. in this study, 21 fluoroquinolone-resistant campylobacters were isolated from poultry samples. morphological and biochemical characteristics indicated that 19 isolates were ...200312619802
antimicrobial resistance of campylobacter jejuni and campylobacter coli isolates from broiler chickens isolated at an irish poultry processing plant.the antibiotic susceptibility of campylobacter jejuni and campylobacter coli isolates from broiler chickens were determined in order to evaluate the level of antibiotic resistance of campylobacter species in the irish poultry industry.200312680938
antimicrobial-resistant campylobacter species from retail raw meats.the antimicrobial susceptibilities of 378 campylobacter isolates were determined. resistance to tetracycline was the most common (82%), followed by resistance to doxycycline (77%), erythromycin (54%), nalidixic acid (41%), and ciprofloxacin (35%). campylobacter coli displayed significantly higher rates of resistance to ciprofloxacin and erythromycin than campylobacter jejuni, and campylobacter isolates from turkey meat showed a greater resistance than those from chicken meat.200312732579
phenotypic and genotypic differentiation of campylobacter spp. isolated from austrian broiler farms: a comparison.an identification scheme based on restriction fragment length polymorphism of polymerase chain reaction products (pcr-rflp) was developed to differentiate isolates of the genera campylobacter, arcobacter and helicobacter. based on the 16s rrna gene of these genera, pcr amplified a 1216-bp fragment. the amplicons were digested with the restriction enzymes rsai and ecorv. additional differentiation was obtained using a pcr-assay based on the hippuricase gene. genotyping was performed on several re ...200312745378
pcr detection of seven virulence and toxin genes of campylobacter jejuni and campylobacter coli isolates from danish pigs and cattle and cytolethal distending toxin production of the isolates.to study the prevalence of seven virulence and toxin genes, and cytolethal distending toxin (cdt) production of campylobacter jejuni and c. coli isolates from danish pigs and cattle.200312752808
rapid detection of campylobacter coli, c. jejuni, and salmonella enterica on poultry carcasses by using pcr-enzyme-linked immunosorbent assay.contamination of retail poultry by campylobacter spp. and salmonella enterica is a significant source of human diarrheal disease. isolation and identification of these microorganisms require a series of biochemical and serological tests. in this study, campylobacter ceue and salmonella inva genes were used to design probes in pcr-enzyme-linked immunosorbent assay (elisa), as an alternative to conventional bacteriological methodology, for the rapid detection of campylobacter jejuni, campylobacter ...200312788755
rapid detection of campylobacter jejuni in chicken rinse water by melting-peak analysis of amplicons in real-time polymerase chain reaction.five dna extraction protocols for the detection of campylobacter spp. by polymerase chain reaction (pcr) were compared. a method involving triton x-100 produced template dna of sufficient quality to allow the detection of campylobacter jejuni at levels of 100 cfu/ml in pure culture. primers were designed on the basis of the cadf gene sequence. with a sybr green i real-time pcr assay, these primers amplified only sequences present in c. jejuni to produce a product with a melting temperature of 81 ...200312929818
development of a sensitive dna microarray suitable for rapid detection of campylobacter spp.campylobacter is the most common cause of human acute bacterial gastroenteritis worldwide, widely distributed and isolated from human clinical samples as well as from many other different sources. to comply with the demands of consumers for food safety, there is a need for development of a rapid, sensitive and specific detection method for campylobacter. in this study, we present the development of a novel sensitive dna-microarray based detection method, evaluated on campylobacter and non-campyl ...200312944122
disinfectant susceptibility testing of avian and swine campylobacter isolates by a filtration method.the susceptibility testing of disinfectants against campylobacter jejuni and campylobacter coli strains from broilers and pigs was investigated. the filtration method european standard en 1040 was adapted to campylobacter cultures and validated with reference strains. two disinfectants were tested: 1% benzalkonium chloride active matter, as quaternary ammonium compound, and 0.63% sodium hypochlorite as chlorine-releasing agent. both disinfectants were effective against the 34 campylobacter strai ...200314516706
ribotyping characterisation of campylobacter isolates randomly collected from different sources in italy.in this study the potential for using the automated psti ribotyping as a primary library typing method to survey campylobacter and for identification of two thermophilic campylobacter jejuni and campylobacter coli species was evaluated. a total of 158 isolates randomly collected in italy from different sources were analyzed. a large percentage of chicken (28%), turkey (27%) and turkey meat (25%) isolates shared their ribotyping profiles (ribotypes) with those of humans, whereas the swine isolate ...200314522511
effect of temperature on viability of campylobacter jejuni and campylobacter coli on raw chicken or pork skin.to determine growth and survival of campylobacter jejuni and campylobacter coli on chicken and pork, campylobacter spp. (10(4) cfu/cm2) were inoculated on pieces of raw, irradiated chicken or pork skin and exposed to temperatures ranging from -20 to 42 degrees c under either microaerobic or aerobic conditions. viable counts over 48 h declined 2 to 3 log cfu/cm2 at -20 degrees c and 1 to 2 log cfu/cm2 at 25 degrees c regardless of skin type, species of campylobacter, or level of oxygen. at 4 degr ...200314627278
antimicrobial resistance in campylobacter jejuni and campylobacter coli strains isolated in 1991 and 2001-2002 from poultry and humans in berlin, germany.the susceptibilities of 430 campylobacter jejuni strains and 79 c. coli strains to six antimicrobial agents were tested and analyzed. the two sets of strains originated from retail market chicken and turkey samples and from humans, respectively, in berlin, germany. two groups of isolates, one dating from 1991 and the other dating from 2001-2002, were tested. of the campylobacter sp. isolates recovered from humans in 2001-2002, 45.1% were resistant to ciprofloxacin, 37.8% were resistant to tetrac ...200314638490
fluoroquinolone susceptibility of campylobacter strains, senegal.to assess fluoroquinolone susceptibility of campylobacter strains in senegal, skin samples were collected from 250 chicken carcasses from january 2001 to october 2002. among 205 isolated campylobacter strains, 59% and 41% were identified as campylobacter jejuni and c. coli, respectively; the overall ciprofloxacin-resistance rate was 34%.200314718098
distribution and characterization of campylobacter spp. from russian poultry.the distribution of campylobacter spp. on 13 poultry farms (broiler chicken, quail, pheasant, peacock, and turkey) from eight regions (vladimir, vologda, voronezh, kaluga, liptsk, moscow, orenburg, and orel) in russia was surveyed. intestinal materials were plated onto campylobacter-selective medium and plates were incubated microaerobically at 42 degrees c for 24 or 48 h. identification was based on colonial morphology, microscopic examination, and biochemical tests; latex agglutination assays ...200414968953
fluoroquinolone-resistant campylobacter in animal reservoirs: dynamics of development, resistance mechanisms and ecological fitness.thermophilic campylobacter species, including campylobacter jejuni and campylobacter coli, are responsible for foodborne campylobacteriosis in humans and are increasingly resistant to fluoroquinolone (fq) antimicrobials. the therapeutic use of fq antimicrobial agents in food animal production, particularly in poultry, has become a concern for public health, because the practice may promote the emergence of fq-resistant campylobacter that can be transmitted to humans through the food chain. recen ...200315134291
in vitro inhibitory activity of chinese leek extract against campylobacter species.three aqueous extracts of dietary materials of chinese leek (leek flower stem, soft leek and green leek) and two other reference extracts of allium plants were used to investigate the antibacterial effects against campylobacter jejuni and campylobacter coli. all the tested strains of campylobacter species were isolated from chickens. the minimum concentration of aqueous leek extracts (ales) required to inhibit the bacterial growth was 2.0 mg/ml. among the plant extracts tested, ales shared the l ...200415193803
quinolone-resistant campylobacter infections: risk factors and clinical consequences.we integrated data on quinolone and macrolide susceptibility patterns with epidemiologic and typing data from campylobacter jejuni and c. coli infections in two danish counties. the mean duration of illness was longer for 86 patients with quinolone-resistant c. jejuni infections (median 13.2 days) than for 381 patients with quinolone-sensitive c. jejuni infections (median 10.3 days, p = 0.001). foreign travel, eating fresh poultry other than chicken and turkey, and swimming were associated with ...200415207057
occurrence of campylobacter in retail foods in ireland.a surveillance study was carried out to determine the prevalence of campylobacter in a range of retail foods purchased in three irish cities over a 20-month period between march 2001 and october 2002. in total 2391 food samples were analysed during this period. campylobacter was isolated from 444 raw chicken (49.9%), 33 turkey (37.5%) and 11 duck samples (45.8%). lower isolation rates of 7/221 (3.2%), 10/197 (5.1%) and 31/262 (11.8%) were observed for raw beef, pork and lamb, respectively. one s ...200415282123
antigenicity of the campylobacter coli cjaa protein produced by escherichia coli.immunogenic c. coli cjaa protein is a candidate for a chicken anti-campylobacter subunit vaccine. in order to enhance its immunogenicity an antigenic determinant of the cjaa protein was identified. thereafter, two copies of the antigenic epitope were cloned in tandem directly or with a flexible hinge between them. all experiments documented that the cjaa protein contained not only linear antigenic epitope/s but also conformational ones.200415330269
species identification by genotyping and determination of antibiotic resistance in campylobacter jejuni and campylobacter coli from humans and chickens in sweden.campylobacter is today the most common cause of human bacterial enteritis in sweden, as well as in most other industrialized countries. common sources of infection are undercooked chicken meat, unpasteurized milk and contaminated drinking water. one aim with our present study was to identify the species campylobacter jejuni and campylobacter coli strains from humans and chickens using a polymerase chain reaction/restriction enzyme analysis (pcr/rea) method, as well as traditional hippurate hydro ...200415364471
use of culture, pcr analysis, and dna microarrays for detection of campylobacter jejuni and campylobacter coli from chicken feces.a dna microarray for detection of campylobacter spp. was recently developed and applied to detect campylobacter spp. directly from chicken feces. sixty-five pooled chicken cloacal swab samples from 650 individual broiler chickens were included in the study. the results of campylobacter sp. detection obtained with dna microarrays were compared to those obtained by conventional culture and gel electrophoresis. by conventional culture, 60% of the samples were positive for either campylobacter jejun ...200415364980
a european survey of antimicrobial susceptibility among zoonotic and commensal bacteria isolated from food-producing animals.to study antimicrobial resistance in zoonotic bacteria isolated from food animals in different countries using uniform methodology.200415375107
antimicrobial resistance in campylobacter strains isolated from french broilers before and after antimicrobial growth promoter bans.the antimicrobial susceptibility of campylobacter strains isolated from standard and free-range broilers in 1992-1996 and 2001-2002 was studied.200415537699
sequence typing and comparison of population biology of campylobacter coli and campylobacter jejuni.a multilocus sequence typing (mlst) scheme that uses the same loci as a previously described system for campylobacter jejuni was developed for campylobacter coli. the c. coli-specific primers were validated with 53 isolates from humans, chickens, and pigs, together with 15 penner serotype reference isolates. the nucleotide sequence of the flaa short variable region (svr) was determined for each isolate. these sequence data were compared to equivalent information for 17 c. jejuni isolates represe ...200515634992
rapid detection and differentiation of pathogenic campylobacter jejuni and campylobacter coli by real-time pcr.a two-tube real-time assay, developed in a lightcycler, was used to detect, identify and differentiate campylobacter jejuni and campylobacter coli from all other pathogenic members of the family campylobacteriaceae. in the first assay, continuous monitoring of the fluorescence resonance energy transfer (fret) signal acquired from the hybridisation of two adjacent fluoroprobes, a specific fitc probe 5'-gtgctagcttgctagaacttagaga-fitc-3') and a universal downstream probe cy5 (5'-cy5-aggtgitgcatggit ...200515636755
tetracycline resistance of australian campylobacter jejuni and campylobacter coli isolates.tetracycline resistance in campylobacter is encoded by the tet(o) gene and is usually associated with conjugative plasmids. little was known about tetracycline resistance in australian campylobacter species, therefore we investigated this resistance in 41 campylobacter jejuni and five campylobacter coli strains from humans and healthy chickens.200515743900
enumeration and diversity of campylobacters and bacteriophages isolated during the rearing cycles of free-range and organic chickens.campylobacters and campylobacter-specific bacteriophages were isolated and enumerated during the rearing cycle of free-range (56 days) and organic chickens (73 days) at 3-day intervals from hatching until slaughter. in both flocks campylobacter jejuni was the initial colonizer but campylobacter coli was detected more frequently from 5 weeks of age. the diversity of the campylobacter isolates was examined by pulsed-field gel electrophoresis of smai-digested genomic dna and antimicrobial resistanc ...200515746327
prevalence of campylobacter, salmonella, and escherichia coli on the external packaging of raw meat.during september and october 2002, 3,662 prepackaged raw meat samples were collected to evaluate the extent and nature of microbiological contamination on external surfaces of the packaging, which could potentially cross-contaminate ready-to-eat foods during and after purchase. salmonella was detected on two (<1%) samples of external packaging (both from raw chicken), and campylobacter was detected on 41 (1.1%) samples of external packaging. the external packaging of game fowl exhibited the high ...200515771168
pcr identification of campylobacter coli and campylobacter jejuni by partial sequencing of virulence genes.the objective of this study was to utilize a multiplex pcr assay for concurrent detection of campylobacter spp. and c. coli or c. jejuni, using probes derived from genes cadf and ceue and an undefined virulence gene. a total of 97 campylobacter strains, isolated from turkey litter (n=74), chicken livers (n=15) and clinical (n=8) samples, were speciated using the pcr-based assay. pcr amplification of the isolates identified a 400-bp cadf gene, conserved in campylobacter species, an 894-bp ceue ge ...200515797819
identification and molecular epidemiology of campylobacter coli isolates from human gastroenteritis, food, and animal sources by amplified fragment length polymorphism analysis and penner serotyping.campylobacter coli is an infrequently studied but important food-borne pathogen with a wide natural distribution. we investigated its molecular epidemiology by use of amplified fragment length polymorphism (aflp)-based genotyping and penner serotyping. serotype reference strains and 177 danish isolates of diverse origin identified by routine phenotyping as c. coli were examined. molecular tools identified some 12% of field isolates as campylobacter jejuni, emphasizing the need for improved ident ...200515812025
mama-pcr assay for the detection of point mutations associated with high-level erythromycin resistance in campylobacter jejuni and campylobacter coli strains.twenty campylobacter jejuni and 16 campylobacter coli strains isolated from humans and food/animals, including 17 isolates resistant to erythromycin, were analyzed. a combined mismatch amplification mutation assay-pcr technique was developed to detect the mutations a 2074 c and a 2075 g in the 23s rrna gene associated with erythromycin resistance. all high-level erythromycin-resistant strains examined by dna sequencing carried the transition mutation a 2075 g, whereas no isolate carried the a 20 ...200515927294
prevalence of potential virulence markers in polish campylobacter jejuni and campylobacter coli isolates obtained from hospitalized children and from chicken carcasses.the pathogenicity of thermotolerant campylobacter species, common food-borne pathogens, depends on certain factors unevenly distributed among strains of different origin. the prevalence of such markers has never been examined in a population of polish campylobacter strains of human and poultry origin. therefore, we analysed the presence of the cadf, cdta, cdtb and cdtc genes and the iam sequence in campylobacter jejuni (n = 115) and campylobacter coli (n = 57) isolates from children with diarrho ...200515947425
inhibition of clostridium perfringens by a novel strain of bacillus subtilis isolated from the gastrointestinal tracts of healthy chickens.the objectives of this study were to isolate beneficial strains of microorganisms from the gastrointestinal tracts of healthy chickens and to screen them against clostridium perfringens, a causative agent of necrotic enteritis in poultry. one of the bacteria isolated, a strain of bacillus subtilis, was found to possess an anticlostridial factor that could inhibit the c. perfringens atcc 13124 used in this study. the anticlostridial factor produced by b. subtilis pb6 was found to be fully or part ...200516085801
factors affecting the pressure resistance of some campylobacter species.to compare pressure resistance between strains of campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter fetus, and to investigate the effect of suspending medium on pressure resistance of sensitive and more resistant strains.200516162138
genomic diversity of campylobacter coli and campylobacter jejuni isolates recovered from free-range broiler farms and comparison with isolates of various origins.in many industrialized countries, the incidence of campylobacteriosis exceeds that of salmonellosis. campylobacter bacteria are transmitted to humans mainly in food, especially poultry meat products. total prevention of campylobacter colonization in broiler flocks is the best way to reduce (or eliminate) the contamination of poultry products. the aim of this study was to establish the sources and routes of contamination of broilers at the farm level. molecular typing methods (dna macrorestrictio ...200516204541
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