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epizootic hemorrhagic disease virus serotype 7 in european cattle and sheep: diagnostic considerations and effect of previous btv exposure.epizootic hemorrhagic disease virus (ehdv), an arthropod-borne orbivirus (family reoviridae), is an emerging pathogen of wild and domestic ruminants that is closely related to bluetongue virus (btv). the present study examines the outcome of an experimental ehdv-7 infection of holstein cattle and east frisian sheep. apart from naïve animals that had not been exposed to btv, it included animals that had been experimentally infected with either btv-6 or btv-8 two months earlier. in addition, ehdv- ...201222560764
bluetongue and epizootic hemorrhagic disease virus isolation from vertebrate and invertebrate hosts at a common geographic site.one serotype of bluetongue virus (btv) and two serotypes of epizootic hemorrhagic disease virus (ehdv) were isolated from vertebrate and invertebrate hosts on a farm in colorado. the isolations were from blood samples collected a week apart from a dairy heifer with stomatitis and laminitis; ehdv serotypes 1 and 2 were isolated from the first blood sample, and btv serotype 13 and ehdv serotype 1 were isolated from the second. antibodies to ehdv and btv were detected in the serum from this heifer. ...19806243617
immunodetection of bluetongue virus and epizoötic hemorrhagic disease virus in culicoides variipennis (diptera: ceratopogonidae).the avidin-biotin complex immunoperoxidase system has been used with nitrocellulose membranes to detect bluetongue virus serotypes 10 (btv-10) and 11 (btv-11) and epizootic hemorrhagic disease virus (ehdv) serotype 1 (ehdv-1) in individual specimens of culicoides variipennis (coquillett). the cross reactivity between btv-10 and btv-11 indicates that polyclonal antisera cannot be used to distinguish between flies infected with btv-10 and btv-11; however, the procedure can be used to discriminate ...19892549249
detection of bluetongue virus in culicoides variipennis (diptera: ceratopogonidae) by an antigen capture enzyme-linked immunosorbent assay.an antigen capture enzyme-linked immunosorbent assay (elisa) was developed to detect bluetongue virus (btv) in the vector culicoides variipennis (coquillett). this elisa used polyclonal and monoclonal antibodies in a biotin-avidin immunoperoxidase system to detect btv in individual adult female flies that had taken an infectious blood meal. detection of btv with the elisa correlated with detection of btv from the same flies by plaque assay in cell culture. the elisa was group-specific for the fi ...19902167373
transmission of two strains of epizootic hemorrhagic disease virus in deer by culicoides variipennis.two strains of epizootic hemorrhagic disease virus (ehdv), new jersey (nj) and kentucky (ky), of deer were biologically transmitted between white-tailed deer, odocoileus virginianus, by culicoides variipennis. the ky strain, isolated from c. variipennis collected during an epizootic in deer, was identified as ehdv by serological tests. deer exposed to the ky or the nj strains of ehdv developed an acute hemorrhagic disease; most deer died 6 to 13 days after infection. sheep inoculated with ehdv d ...1977190424
detection of epizootic hemorrhagic disease virus in culicoides variipennis (diptera: ceratopogonidae).nucleic acid hybridization was used to detect epizootic hemorrhagic disease (ehd) virus serotype 1 and serotype 2 in culicoides variipennis (coquillett). adult females were inoculated intrathoracically with virus, then were assayed daily for the presence of viral rna for 2 wk, at which time maximum virus replication is likely to occur. viral rna of ehd serotypes 1 and 2 was first detected by hybridization on days 9 and 7 after infection, respectively, and then for up to 14 days after infection. ...19911941947
culicoides (diptera:ceratopogonidae) collected during epizootics of hemorrhagic disease among captive white-tailed deer.to help determine specific vectors of epizootic hemorrhagic disease (ehd) and bluetongue (bt) viruses for white-tailed deer, odocoileus virginianus zimmermann, in the southeastern united states, culicoides sp. midges were collected during epizootics of hemorrhagic disease among captive white-tailed deer in georgia, mississippi, and north carolina. culicoides variipennis (coquillett), a confirmed vector of ehd and bt viruses, was present in low numbers in light-trap collections made at all sites. ...19968667402
monitoring of culicoides spp. at a site enzootic for hemorrhagic disease in white-tailed deer in georgia, usa.biting midges of the genus culicoides (diptera: ceratopogonidae) were monitored at a georgia (usa) site where epizootic hemorrhagic disease (ehd) and bluetongue (bt) viruses are enzootic among white-tailed deer (odocoileus virginianus). collections were made using a captive white-tailed deer and light traps from june 1993 through november 1994. we collected 210,482 females from the captive deer during morning and evening periods. predominant species were c. lahillei (73%), c. stellifer (16%), c. ...19969359063
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