divergent results in radial immunodiffusion. iii. isolation and specificity test of class-specific antibodies.a method is described for the isolation of antibodies specific for immunoglobulin class determinants from antisera containing antibodies both for the immunoglobulin class and for its subclasses, using affinity chromatography. the model system used is bovine igg with its subclasses igg1 and igg2, together with antisera raised in goats and sheep. the specificity of the isolated class-specific antibodies was demonstrated by double diffusion (di) and radial immunodiffusion (rid). the levels of total ...197937277
[geopolitical distribution of the occurrence of fasciola hepatica in santa catarina state, brazil].during 12 years feces samples from cows, water buffaloes, sheeps and goats were examined by sequential tamis filtration to show the occurrence of fasciola hepatica eggs. the material came from 129 municipalities of santa catarina state, and 5 g of feces per animal were examined. the occurrence of f. hepatica was confirmed in 64.82% of the municipalities. considering the host, f. hepatica was confirmed in goats from florianópolis, são josé, são joão batista and guaramirim municipalities; in sheep ...19921343793
experimental fascioloides magna infections of mule deer (odocoileus hemionus hemionus).six mule deer (odocoileus hemionus hemionus) and one white-tailed deer (odocoileus virginianus), approximately 5-mo-old, each were inoculated orally with 500 metacercariae of fascioloides magna. all mule deer died from liver fluke infection between 69 and 134 days (mean = 114, se = 9.9) after inoculation. between 38 and 326 immature f. magna (mean = 102, se = 45.5) were recovered from each deer at necropsy. flukes were present in livers, lungs, and free in pleural and peritoneal spaces. infectio ...19921602568
fasciola hepatica infection in china.fasciola hepatica is one of the most common parasites in china that infects ruminants, especially cattle, sheep and goats, and causes considerable morbidity and economic losses in the animal husbandry. the climate in wide areas of china is suitable for the development of f. hepatica and its intermediate snail hosts. however, human infection with the fluke is not common. up to now, 38 hospital patients and six infected individuals from two epidemiological surveys have been reported in the chinese ...19911822926
liver fluke disease (fascioliasis): epidemiology, economic impact and public health significance.liver fluke disease (fascioliasis) is an important parasitic disease found worldwide affecting sheep, goats, cattle and buffalo, as well as other domestic ruminants. the common causative agents are fasciola hepatica and f. gigantica which require various species of lymnaea, fresh water snails, as their intermediate hosts. the epidemiology of the disease and its prevalence in malaysia is mentioned briefly. the disease causes considerable impact on the economy of the livestock industry. the econom ...19911822928
[seasonal variations of fasciola hepatica infection in goats in the area of haouz (morocco)].the prevalence and level of infection with fasciola hepatica were assessed monthly over a 12-month period in goats slaughtered in the haouz area, in correlation with the dynamics of lymnaea truncatula populations. the infection rate was high in winter (23.8%) in january), and summer (17.1% in august), and relatively low in spring and at the beginning of autumn. the infected animals had a fluke burden range of 1-48; a mean of 13 was recorded in july. lymnaeid snails were observed in their habitat ...19911897872
immunohistochemical study on the antigenicity of each organ structure of clonorchis immunohistochemical study was performed to demonstrate comparative antigenicity of each body structure of the liver fluke, clonorchis sinensis, such as the digestive tract, reproductive organs, excretory system, tegument and suckers. indirect immunoperoxidase technique was applied, using formalin-fixed and paraffin-embedded sections of c. sinensis as the antigen. pooled cat sera obtained 10 weeks after an experimental infection with c. sinensis and peroxidase-conjugated goat anti-cat igg were ...19911911624
efficacy of clorsulon for the treatment of experimentally induced infections of fasciola hepatica in goats.a dose titration study was undertaken to determine the efficacy of clorsulon against the adult stage of fasciola hepatica in goats. thirty-nine goats were experimentally infected with metacercariae of f hepatica. at 14 weeks after infection, each goat was assigned randomly to 1 of 5 groups. goats in groups 1 to 4 received a single oral administration of clorsulon at dosages of 3.5, 7, 11, and 15 mg/kg of body weight, respectively. the fifth group of goats (control group) was infected with f hepa ...19912021237
[studies on the karyotypes of fasciola spp].the karyotypes of diploid and triploid forms of the common liver fluke were analysed by air-drying method. the chromosome number of diploid form was 2n = 20, n = 10, and the karyotype consists of the chromosomes with 2m, 4sm, 3st, 1t. there were a number of similarities between the karyotypes of diploid form and triploid form except that the chromosome number of the triploid was 3n = 30 and n = 10. judging from the shape of adult worms and their life cycle, the worm of the diploid form is simila ...19902208626
some pharmacokinetic data of the liver-fluke anthelmintic nitroclofene in ruminant and pre-ruminant kids. 19892614861
isoenzyme patterns of phosphatases and esterases in fasciola hepatica and dicrocoelium dendriticum.a study was made of alkaline and acid phosphatase isoenzymes and non-specific esterases in homogenates of the trematodes fasciola hepatica and dicrocoelium dendriticum obtained from infected tissues of capra hircus and ovis aries using horizontal polyacrylamide gel electrophoresis. the esterase patterns in f. hepatica and d. dendriticum were different. homogenate of f. hepatica from both hosts gave four enzyme bands. for d. dendriticum, however, while homogenates of parasites from c. hircus also ...19892728320
pharmacokinetics and efficacy of triclabendazole in goats with induced fascioliasis.the pharmacokinetics of triclabendazole were evaluated in normal goats and in goats artificially infected with fasciola hepatica. triclabendazole and its metabolites were determined using a novel high performance liquid chromatographic method with fluorimetric detection after solid-phase extraction. in normal goats triclabendazole given orally was metabolized rapidly to its sulphoxide and sulphone derivatives. the maximum plasma concentrations for the sulphoxide and sulphone were similar ranging ...19883184265
fasciola hepatica and dicrocoelium dendriticum: isoenzyme patterns of malate dehydrogenase and malic enzyme.the mdh isoenzymes and me isoenzymes (ec. in two helminth parasites, f. hepatica and d. dendriticum, obtained from two host species (capra hircus and ovis aries), were studied by electrophoresis on polyacrylamide gels. f. hepatica mdh showed three isoenzymatic bands; d. dendriticum mdh, only two. no difference was noted between the enzyme profiles, or the densitometric scans in samples from specimens from different host species. f. hepatica me presented three bands, that of d. dendritic ...19883202330
efficacy and safety of albendazole against experimentally induced fasciola hepatica infections in goats.forty 8-week-old goats were allocated to five groups of equal size to determine the optimal dosage of albendazole against experimentally induced 14-week-old fasciola hepatica infections. albendazole suspension given orally at 5.0, 7.5, 10.0 and 15 mg kg-1 of body weight was 73.3, 88.2, 88.3 and 95.9% effective, respectively, when compared to untreated controls. mean number of f. hepatica in the untreated control goats was 75.4. no signs of toxicity were observed. when albendazole was given to ei ...19883347986
epidemiology and control of trematodes in small ruminants. 19863488109
resistance to fascioliasis--a review.attempts to actively stimulate or passively transfer resistance to fasciola hepatica or f. gigantica in various laboratory and farm animals including mice, rats, rabbits, sheep, goats and cattle have been reviewed. these attempts comprised sensitization by primary homologous or heterologous normal or irradiated infections per os, sensitization by subcutaneous, intramuscular or intraperitoneal implantation with the various fluke stages, sensitization by somatic extracts or metabolic products of m ...19863518218
isoenzymes of lactate dehydrogenase (ec in dicrocoelium dendriticum and fasciola hepatica (trematoda).the isoenzymes of ldh (ec were studied in dicrocoelium dendriticum and fasciola hepatica by horizontal electrophoresis on polyacrylamide gel. six isoenzymes in d. dendriticum and four in f. hepatica were detected. densitometric scans demonstrated that the enzyme pattern of ldh in d. dendriticum parasitizing hepatic tissue of capra hircus was clearly different from the one obtained when the trematode parasitized other hosts such as bos taurus or ovis aries.19863943300
the susceptibility of the goat to fasciola hepatica infections.mixed breed goats were infected with metacercariae of fasciola hepatica and the resulting worm burdens were quantitated after primary and secondary exposure of the goats to the parasite. mean length and width of the parasite recovered after all primary exposures were 1.91 +/- 0.2 cm and 0.91 +/- 0.2 cm, respectively. a mean of 71.8 +/- 5.9% of the flukes were recovered from all of the primary infections. in the secondary infections, the mean length and width of the flukes from the physically sma ...19863962156
the detection of liver cell damage by plasma enzyme changes in goats given immature fasciola hepatica directly into the biliary system. 19744820084
fasciola hepatica: influence of extrahepatic adult flukes on infections and immunity in rats. 19744826983
a review of the antigens of fasciola hepatica.the interaction between the antigens of fasciola hepatica and the host immune response are reviewed. this paper evaluates not only more recent work, but the older literature as well. antigens from each stage in the life cycle are considered with the idea of identifying those antigens with a potential for use in an effective vaccine. antigens which cross-react with other parasite species are detailed as well as those that cross-react between different stages in the life cycle of f. hepatica. the ...19846382779
efficacy of triclabendazole against fasciola hepatica in sheep and goats.triclabendazole (fasinex), a new fasciolicide of the benzimidazole group, was tested in sheep and goats. a controlled test with 24 artificially-infected sheep revealed a 100% efficacy of triclabendazole at a dose of 10 mg kg-1 body weight against fasciola hepatica aged 4 and 13 weeks, respectively. in naturally-acquired severe subacute to chronic fascioliasis in 66 sheep and 10 goats, the drug was highly effective in three trials when applied at 10 mg kg-1 in sheep and at 5 mg kg-1 in goats. tri ...19836685399
protection against fasciola hepatica in rats with adult fluke antigen in freund's adjuvant: influence of antigen batch, antigen dose and number of sensitising of rats against fasciola hepatica infection induced by intraperitoneal injection of adult fluke antigen in freund's adjuvant was dependent on the batch of antigen. relatively modest protection of 41 per cent and 55 per cent provided by some batches at one dose could be improved to 65 to 84 per cent and 86 per cent respectively by increasing the amount of antigen used for sensitisation. protection was found to require at least two sensitising injections. subcutaneous sensitisation with ...19836856998
clinical fascioliasis in domestic goats in montana.fascioliasis (fasciola hepatica infection) was diagnosed in a herd of domestic goats in montana. twenty-eight goats died after a month-long clinical course of anorexia, weight loss, depression, lethargy, and decreased milk production. clinical laboratory findings included anemia, low hemoglobin content, hypoproteinemia, hypoalbuminemia, eosinophilia, and high hepatic enzyme activity. the livers of affected goats had extensive parenchymal necrosis, fibrosis, and biliary hyperplasia. albendazole o ...19827096186
experimental infection of domestic goats with fascioloides magna.development of the deer liver fluke, fascioloides magna, was studied in seven domestic goats, each experimentally inoculated with 250 metacercariae. six infected goats died between 89 and 195 days (mean 139) after inoculation. infection did not develop in the seventh goat. mortality was the result of unrestricted fluke migration before maturation or encapsulation of the flukes. a total of 144 f magna (mean intensity of 24.0) were recovered from inoculated goats, representing 8.2% of the metacerc ...19807436074
early immunodiagnosis of caprine fasciolosis using the specific f2 antigen in a passive hemagglutination improved hemagglutination (ha) test using the purified specific f2 antigen of fasciola hepatica has been evaluated with respect to its potential use in the diagnosis of caprine fasciolosis. following experimental infection of 1-year-old goats with a single heavy infection of 300 metacercariae, f2-specific antibodies were detected 2-3 weeks after infection and increased steadily to reach a maximum titer 9 weeks after infection, after which the antibody level declined. in animals receiving mult ...19947522368
development and application of a dot-elisa test for the detection of serum antibodies to fasciola hepatica antigens in llamas.a microenzyme-linked immunosorbent assay (dot-elisa) was developed to detect serum antibodies against fasciola hepatica antigens in llamas. sera from five f. hepatica-infected and 11 non-infected llamas were used in initial test development. nitrocellulose filter disks containing f. hepatica excretory-secretory product were placed in 96-well microtiter plates, washed, blocked with tween-20, then incubated with four-fold serial dilutions of llama sera. after incubation with rabbit anti-llama igg ...19957676604
liver fluke infections in livestock in borno state, nigeria.a survey was conducted on the prevalence of fasciola gigantica and dicrocoelium hospes eggs in gall bladders of cattle and small ruminants (sheep and goats) slaughtered between november 1987 and december 1988 in maiduguri, borno state, nigeria. prevalence rates of 42.7% and 5.7% were recorded for f. gigantica and d. hospes eggs, respectively, in 246 cattle and 718 sheep and goats examined. out of the 246 cattle examined, 52 (21.1%), 45 (18.3%) and 8 (3.3%) excreted eggs of f. gigantica, d. hospe ...19938122358
immunodiagnosis of taenia saginata in cattle using hydrophobic antigens from t. hydatigena metacestode cyst fluid.hydrophobic fractions isolated from cyst fluid of taenia hydatigena metacestodes, obtained from naturally infected goats from tanzania, were used in an indirect elisa and in immunoblot (western or dot blot) procedures. the use of the most hydrophobic fractions of the cyst fluid proved to result in a better diagnostic discrimination in elisa than the more hydrophilic fractions. best diagnostic ratio between responses from t. saginata infected and non-infected cattle was achieved when using horse- ...19958541896
detection of antibodies of fasciola hepatica excretory--secretory antigens in experimentally infected goats by enzyme immunosorbent elisa with excretory-secretory (es) antigens has been evaluated as a technique for the early detection of specific antibodies in fasciola hepatica infections in goats. goats were experimentally infected with 100 or 200 metacercariae of bovine origin and serum samples were taken periodically over 365 days. the elisa test was performed with es antigens (10 micrograms ml-1), a single dilution of sera (1:800) and anti-goat igg conjugate (1:1000). es specific antigens were detected in all infected ...19968686171
cross-reactive, stage-specific antigens in the oestridae family.the larval stages of different economically important oestridae species were studied for their antigenicity and cross reactivity, using elisa and immunoblotting. the immune sera of cattle from algeria, belgium, france and switzerland were compared for their capacity to recognize the stage-specific antigens of their specific parasites hypoderma bovis and h lineatum, originating from different populations. this comparison was extended to other hypoderminae species responsible for economic losses i ...19968822621
seasonal transmission of fasciola hepatica in montana, usa, with detection of infected intermediate hosts using a dna-based assay.a cashmere goat ranch in the beaverhead valley, southwest montana, usa reported acute fasciolosis in march 1992. the ranch was used as a study site to gather seasonal transmission data for the liver fluke, fasciola hepatica. testing of snails for infection with a nucleic acid-based assay, and use of tracer sheep at the site has shown the seasonal transmission of the parasite is only in late autumn. the snail responsible for transmission was identified as lymnaea modicella although another known ...19968983156
efficacy of closantel against fasciola hepatica in korean native goats.closantel (flukiver), a salicylanilide antiparasitic compound, was tested in korean native goats infected with fasciola hepatica. the goats were administered closantel once orally at a dose of 10 mg/kg body weight. efficacy was monitored weekly by fecal examination of all infected animals starting the second week post-treatment and continuing for 3 weeks. closantel elicited 80.3, 97.8 and 92.7% efficacy in goats with naturally-acquired fasciolosis at the second, third and fourth week post-treatm ...19968983157
a focus of fasciola hepatica in crete without human greece, fasciola hepatica, the sheep liver fluke, is common in sheep but only three human cases of fascioliasis have been reported. an epidemiological study was conducted in central crete which proved to be a focus of f. hepatica. sheep and snails were found positive for this parasite, but none of the 205 persons tested serologically.19979062787
triclabendazole treatment in experimental goat fasciolosis: anthelmintic efficacy and influence in antibody response and pathophysiology of the disease.a controlled test of the efficacy of triclabendazole against all stages (early immature, late immature and mature) of fasciola hepatica has been performed in experimentally infected goats. the influence of triclabendazole treatment on the pathophysiology of the disease, in terms of haematological parameters and serum enzyme levels, and in the dynamics of production of specific antibodies to excretory/secretory products (esp) of f. hepatica were also examined. goats were orally infected with 200 ...19979066052
humoral and cellular immune responses to experimental fasciola hepatica infections in goats.humoral and cellular immune responses to fasciola hepatica excretory-secretory products (esps) in primary and secondary experimental infections in goats were studied. primary infection induced the development of chronic subclinical fascioliasis that did not affect the establishment of flukes coming from the secondary infection, as the same percentages of recovered flukes were found in both groups. the specific igg response to f. hepatica esps was similar in primary and secondary infections; chal ...19979272558
immunohistochemical study of the local immune response to fasciola hepatica in primarily and secondarily infected goats.the distribution of cd2, cd4, cd8, gamma/delta t-lymphocytes, b-cells and igg lambda-light chain (lambda-igg) containing cells were analysed in the inflammatory infiltrate associated to hepatic lesions and gallbladder (hl), and in hepatic lymph nodes (hln) of goats primarily and secondarily infected with fasciola hepatica. in the hl, cd2 and cd8 t-cells were more numerous (p=0.01) in secondarily rather than in primarily infected goats, whereas cd4 t-lymphocytes were less numerous than cd8 and sh ...19989764726
fasciola hepatica: partial characterization of circulating antigens.antigens present in sera from fasciola hepatica-infected goats were partially characterized by means of polyacrylamide gel electrophoresis of immunoprecipitates obtained by counterimmunoelectrophoresis. agar containing the 2 immunoprecipitin lines, agar containing no precipitin lines, a crude extract of f. hepatica, and normal rabbit igg were assayed. the electrophoretic pattern of 1 precipitin line showed, apart from the light and heavy chains of igg and other faint bands, 2 prominent polypepti ...19989794656
pathological and immunohistochemical study of the liver and hepatic lymph nodes in goats infected with one or more doses of fasciola hepatica.lesions produced by fasciola hepatica in the liver, gall-bladder and hepatic lymph nodes (hlns) of four groups of five goats are described; in addition, the distribution of cd3+ t lymphocytes and igg-lambda light chain-bearing cells was analysed in the hepatic lesions and hlns. one group of goats received a single oral dose of metacercariae, but the other four groups received four or five doses at different intervals over a period of 11 weeks. animals that survived were killed 53-55 weeks after ...199910087493
liver pathology and immune response in experimental fasciola hepatica infections of goats.the relationship between the immune response and the pathogenesis of the disease was studied in different primary and secondary experimental fasciola hepatica infections of goats. the establishment of the infection, measured as percentage of recovered flukes at the necropsy, was similar in primarily and secondarily infected animals (between 19.7% and 24.3%), but the hepatic damage was much more severe in secondarily infected goats, as revealed by the levels of serum hepatic enzymes ggt and ldh. ...199910223347
camel: a new egyptian host for fasciola gigantica.f. gigantica is a known liver fluke in egypt. infection has been reported in sheep, goats, buffaloes, cows and donkeys. human cases have been reported from several governorates. this paper records camels as an additional host for f. gigantica in egypt. in such materials worms attained a length of 6.0-6.3 cm.200010946507
oxidative responses during bacterial phagocytosis of polymorphonuclear leucocytes in primarily and secondarily fasciola hepatica infected goats.the polymorphonuclear leukocyte (pmn) function, in terms of oxidative response during bacterial phagocytosis, was studied using a luminol-dependant chemiluminiscence (ldcl) assay in primarily and secondarily fasciola hepatica infected goats. polymorphonuclear leukocytes of f. hepatica infected goats displayed lower ldcl responses than naive goats. the lowest responses were observed in secondarily infected animals that had higher parasitic burdens and more prominent hepatic lesions. the reduced r ...200010980292
the contribution of molecular biology to the development of vaccines against nematode and trematode parasites of domestic ruminants.rapid developments in molecular biology have had an enormous impact on the prospects for the development of vaccines to control the major nematode and trematode infestations of livestock. vaccine candidates are purified using conventional protein chemistry techniques but the limitations imposed by the scarcity of parasite material provide an insurmountable barrier for commercial vaccine production by this means. the ability to purify mrna from different parasite life-cycle stages and to prepare ...200111707304
a review of dicrocoeliosis of ruminants including recent advances in the diagnosis and treatment.despite its widespread presence among grazing ruminants, dicrocoeliosis, also known as "small liver fluke" disease, is poorly known and often underestimated by researchers and practitioners in many countries. this is primarily due to the multiple parasitic infections which affect ruminant livestock and mask the pathology of dicrocoeliosis, to the difficulties in diagnosing it with coprological techniques and, finally, to the few effective drugs found. furthermore, the biological cycle of dicroco ...200212163243
abattoir condemnation due to parasitic infections and its economic implications in the region of trikala, greece.the prevalence of parasitic infections responsible for the condemnation of carcasses and viscera during meat inspection, and their economic implication, was estimated in a year long abattoir survey of 10 277 slaughtered farm animals in the region of trikala, greece. the organs examined for the presence of parasitic lesions during meat inspection were: liver and lungs of all animals, rumen of cattle, small intestine of lambs and kids, and muscles of cattle and swine. the parasitic lesions observe ...200212241028
helminth fauna of cervids in belorussian polesie.we report on the examination of 18 elk ( alces alces), 16 red deer (cervus elaphus)and 16 roe deer ( capreolus capreolus) from the belorussian polesie in the period 1981-1998 for helminths. a total of 18 helminth species were found including dictyocaulus eckerti, fasciola hepatica, oesophagostomum venulosum, taenia hydatigena larvae and trichuris ovis, all of which occurred in all host species. sixteen of the species found are known to infect humans, domestic animals and/or farm animals.200312474047
a preliminary study on dicrocoeliasis in egypt, with a general review.dicrocoelium dendriticum (d. dendriticum) is a lancet-shape liver fluke that affects sheep and mammals including man. human and animal infections have been world widely reported particularly in some of the eastern mediterranean sheep raising countries. infection is acquired by eating the second intermediate host (17 species of ants) with raw fruits, vegetables, herbs or even with the drinking water. as for the first intermediate host, there is about 54 different species of land snails. in spite ...200312739803
human infection with dicrocoelium dendriticum in riyadh district (saudi arabia).dicrocoelium dendriticum (the lancet fluke) is less common liver parasite. adults inhibit bile duct, while characteristic eggs pass in stools. during one academic year, a total of 1196 patients referred to parasitology laboratory from king abdel aziz university hospitals, 121 patients were positive for d. dendriticum. the majority was saudis, and only 32 of the 121 patients (26.4%) had true dicrocoeliosis, as detected by re-examination after three days of liver free diet. the other patients have ...200312739807
humoral response (igg) of goats experimentally infected with fasciola hepatica against cysteine proteinases of adult fluke.the use of cysteine proteinases from fasciola hepatica adult flukes for the serodiagnosis of caprine fasciolosis by means of an indirect elisa test was studied. two proteolytic fractions from adult fluke homogenates, with apparent molecular weights of 28 and 34 kda (p28 and p34 respectively), were characterised as cysteine proteinases using azocasein assays and gelatin gel analysis. both p28 and p34 fractions were electroluted and used as antigens in two different indirect elisa tests. serum igg ...200312911860
[results of parasitological examinations of faecal samples from horses, ruminants, pigs, dogs, cats, hedgehogs and rabbits between 1998 and 2002].the results of coproscopical examinations in horses, ruminants, pigs, dogs, cats, hedgehogs and rabbits between 1998 and 2002 are presented. in 4399 samples from horses 37.4% stages of strongylids, 1.4% anoplocephalids, 1.3% strongyloides westeri, 0.9% parascaris equorum, 0.04% oxyuris equi, 0.04% eimeria sp. and 0.04% fasciola hepatica were found. in 998 samples of cattle 22.1% stages of strongylids, 11.2% of eimeria spp., 3.5% of cryptosporidium, 2.9% of moniezia spp., 1.3% of trichuris spp., ...200415287577
human and animal fascioliasis in mazandaran province, northern iran.the fascioliasis situation in humans and livestock of iranian mazandaran is analysed for the first time. coprological studies showed 7.3% and 25.4% global prevalences in sheep and cattle, respectively. studies in slaughterhouses indicate that sheep and cattle may be the main reservoir species, buffaloes may play local roles in the transmission, and goats and horses probably only participate sporadically. morphometric studies by computer image analysis showed that forms intermediate between fasci ...200415338292
characterization of specific and cross-reacting antigens of fasciola gigantica by immunoblotting.somatic antigens of f. gigantica, g. explanatum, s. spindale and hydatid cyst ingredients were analysed to identify the cross-reactive antigens among them using western blot technique. when probed with f. gigantica infected cattle sera, the immunodominant 156 kda and 28 kda proteins of f. gigantica was found common amongst the antigens prepared from hydatid cysts ingredients like germinal layer, fertile and sterile, hydatid fluid, fertile and sterile, while another protein of 34 kda was shared b ...200515952043
[four cases of hepatic fascioliasis mimicking cholangiocarcinoma].human fascioliasis is a zoonosis caused by fasciola hepatica, and this is a trematode that infests cattle and sheep. humans are accidental hosts in the life cycle of this parasite. people are infected by ingestion of the water or the raw aquatic vegetables that are contaminated with the metacercaria. this fluke's worldwide distribution occurs in areas where sheep, cattle and goats are raised, and there is a life-cycle niche for this fluke in lymnaeid snails. however, it is a rare disease in kore ...200515980676
nematodes and liver fluke in new zealand.a general review of the epidemiology, significance and control of nematode parasitism of sheep, goats, cattle and deer in new zealand, the emergence of anthelmintic resistance and its effects, and the search for parasite control strategies that reduce reliance on anthelmintic use, is provided. the research that has formed the basis for present levels of understanding of this complex and important topic is summarised and sources of further information are indicated. aspects of nematode infections ...200216032235
[a focus study from a case of human fascioliasis in neuquén].an epidemiological focal study was performed in loncopué, neuquén, argentina, in november 2002 to detect the origin of the infection in a human case of fascioliasis confirmed by an indirect-elisa test, six months before the study. thirty five individual fecal samples were taken from domestic livestock, and watercress plants and snails were collected from the irrigation ditches connected to a main canal in the surroundings of the patient's house. a new blood sample was taken from the already reco ...200516042130
an unusual case of biliary obstruction caused by dicrocoelium dentriticum.dicrocoelium dentriticum (d. dentriticum) is a liver fluke induced biliary obstruction. infection usually occurs in herbivores such as sheep, goats and deer; human infection is very rarely encountered in clinical practice. we report on a 65-y-old female presenting with biliary obstruction caused by d. dentriticum. following treatment with triclobendazole, her symptoms disappeared, and laboratory values returned to normal range within 6 months. parasitosis is an important cause of biliary obstruc ...200516051581
a retrospective survey of liver fluke disease in livestock based on abattoir data in shiraz, south of iran.meat-inspection records in an abattoir located in the fars province (southern part of iran) from 20 march 1999 to 19 march 2004 were used to determine the prevalence and long-term trend of liver fluke disease in sheep, cattle and goats in the region. a total of 844,039 animals (cattle 131,716; sheep 577,090; goats 135,233) slaughtered in the 5-year period and overall 34,856 (4.1%) livers were condemned. fasciolosis and dicrocoeliosis were responsible for 54 and 21% of total liver condemnations, ...200616198433
prevalence and significance of gastrointestinal helminths and protozoa in south american camelids in switzerland.a cross sectional study was conducted to determine the prevalence and significance of endoparasitic infections in south american camelids (sac) in switzerland. qualitative and quantitative coproscopic examinations were performed in 38 farms during the grazing period. management practices with possible interference with parasitic infections were analyzed. on the farm level prevalences of endoparasitic infections were: trichostrongyles 87%; trichuris sp. 74%; capillaria sp. 68%; nematodirus battus ...200617009711
the prevalence and economic significance of fasciola gigantica and stilesia hepatica in slaughtered animals in the semi-arid coastal kenya.fasciola gigantica and stilesia hepatica constrain ruminant productivity. the prevalence and economic losses caused by f. gigantica and s. hepatica in the ruminant production systems of taveta division of kenya were estimated in a retrospective appraisal of the slaughter records on the total number of animals slaughtered and livers condemned over the period 1989 to 2004. only liver condemnations based on infestation by f. gigantica and s. hepatica were considered for purposes of this study. live ...200617243475
humoral immune responses during experimental infection with fascioloides magna and fasciola hepatica in goats and comparison of their excretory/secretory products.this study investigated the humoral immune responses of goats experimentally infected with fascioloides magna and fasciola hepatica to f. magna excretory/secretory products (fmesp) or f. hepatica excretory/secretory products (fhesp), respectively. an enzyme-linked immunosorbent assay (elisa) was used to determine serum antibody responses and for possible discrimination of f. magna and f. hepatica infections in goats. comparison of esps of both flukes and evaluation of esp antigenicity was also s ...200717265088
prevalence of fasciola hepatica (elisa and fecal analysis) in ruminants from a semi-desert area in the northwest of mexico.the aim of the present study was to estimate the prevalence of fasciola hepatica in cattle, goats and sheep from the municipalities of guaymas and cajeme, sonora state, mexico using the indirect elisa and benedeck's sedimentation tests. a total of 2,936 serum and fecal samples from 1,346 bovines, 1,199 goats and 381 sheep were analyzed. other parameters such as breed and age were also taken into consideration. the results indicated a moderate percentage of prevalence for fasciolosis in these mun ...200717265091
sequence analysis of the first internal transcribed spacer of rdna supports the existence of the intermediate fasciola between f. hepatica and f. gigantica in mainland the present study, a polymerase chain reaction-linked single-strand conformation polymorphism (pcr-sscp) approach combined with dna sequencing was used to characterise samples of fasciola spp. from different host species and geographical locations in mainland china. the first internal transcribed spacer (its-1) of ribosomal dna (rdna) was amplified by pcr from individual fasciola and analysed by sscp. sscp analyses displayed three different banding profiles that allowed the identification of ...200717356892
ectopic fascioliasis mimicking a colon tumor.fasciola hepatica, a leaf shaped trematode that is common in cattle, sheep and goats, is acquired by eating raw water plants like watercress or drinking water infected with the encysted form of the parasite. the varied clinical presentations of fascioliasis still make a high index of suspicion mandatory. besides having a wide spectrum of hepatobiliary symptoms like obstructive jaundice, cholangitis and liver cirrhosis, the parasitic infection also has extrabiliary manifestations. until recently, ...200717552017
epidemiological studies on fasciola hepatica in gafsa oases (south west of tunisia).epidemiological investigations on fasciola hepatica fasciolasis were carried out from july 2004 to june 2005 in the gafsa oases (tunisia) after the detection of a human case. three habitats were studied: one in el gsar and two in ain soltan. the prevalence of human infection was 6.6%. the presence of the parasite was detected through serology in 14.3% of cattle, 35% of sheep and 68.4% of goats. the plants apium nodiflorum, oxalis cernua and sonchus maritimus were suspected to be at the origin of ...200717933307
prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern ethiopia.a 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (gi) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern ethiopia. during may 2003 to april 2005, viscera including liver, lungs and gi tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of haramaya, harar, dire dawa and jijiga in eastern ethiopia. all animals were raised in the farming areas ...200717969715
epidemiology of fasciolosis affecting iberian ibex (capra pyrenaica) in southern spain.between 1995 and 2006, we surveyed the presence of fasciola hepatica in iberian ibex (capra pyrenaica) from andalucía (southern spain) by both necropsy (n = 2,096) and coprological approaches (n = 380). most of the samples came from the sierra nevada mountain range (n = 1,884 and 267, respectively), and all positive cases involved animals from this location. the prevalence reached 0.53% by necropsy and 1.87% by faecal examination. taking into account both diagnostic methodologies and the total n ...200818092179
[hepatobiliary fascioliasis and echinococcosis/hydatidosis in domestic animals in haiti].in haiti, hepatobiliary fascioliasis and hepatic hydatid cysts cause major economic losses among livestock. surveys show high prevalence rates for bovine distomatosis caused by fasciola hepatica (10.7% to 22.78%). among small ruminants, the prevalence of distomatosis is low (sheep: 3.2%, goats: 0.9%) although dicrocoelium dendriticum is found in 1.1% of sheep. hepatic hydatidosis is more common among pigs (5.2%) and sheep (2.1%) than among goats (0.9%) and cattle (0.3%). in the case of dogs, 21% ...200718293622
elucidation of genetic variability among different isolates of fasciola gigantica (giant liver fluke) using random-amplified polymorphic dna polymerase chain reaction.the present communication focuses on molecular characterization of fasciola gigantica isolates derived from cattle, buffalo, and goat using random-amplified polymorphic dna (rapd)-polymerase chain reaction (pcr) analysis to elucidate genetic variability between the three isolates. seventeen random oligonucleotide primers of 10-11 bases with gc content varying from 50-81.8% were used in the study. depending upon the f. gigantica isolate-primer combination, one to five fragments in the range of 32 ...200818626662
a study of the liver of goats immunized with a synthetic peptide of the sm14 antigen and challenged with fasciola hepatica.a synthetic peptide of the sm14 antigen of schistosoma mansoni was used to immunize goats against experimental challenge with fasciola hepatica. the goats used consisted of: group 1 (unimmunized and uninfected [controls]); group 2 (unimmunized and infected); group 3 (immunized and infected). compared with group 2, the animals of group 3 showed at necropsy a reduction in hepatic worm burden; however, because of variability in the two groups this reduction was not statistically significant. a gros ...200818768187
[prevalence of liver flukes in hair goats slaughtered in hakkari (yüksekova) province].the aim of this investigation was to determine the prevalence of distomatosis in 267 hair goats which were slaughtered in the slaughterhouses belonging to butchers located in yüksekova district of hakkari province. in the study, distomatosis were detected in 165 (61.80%) of the 267 hair goats. distomatosis was found in 20/98 (20.41%) hair goats which were younger than one year old and in 145/169 (85.80%) which were older than one year. it was found that the species most often responsible were fa ...200819156606
study of the local immune response to fasciola hepatica in the liver and hepatic lymph nodes of goats immunised with a peptide of the sm14 antigen.the nature of the local immune response was assessed studying the distribution of cd2(+), cd4(+), cd8(+), gammadelta(+) t lymphocytes, igm(+) b cells, il-4(+) and ifn-gamma(+) cells in the liver and hepatic lymph nodes (hln) of goats immunised with a synthetic peptide of the sm14 antigen from schistosoma mansoni and challenged with fasciola hepatica. a morphometric study of hln was also carried out in order to evaluate the hyperplasia of lymphoid follicles. despite the decrease in fluke burdens ...200919345386
pathological and parasitological protection in goats immunised with recombinant cathepsin l1 and challenged with fasciola hepatica.fluke burdens, faecal egg output and hepatic damage were assessed in goats immunised with recombinant cathepsin l1 (rcl1) plus quil a (n=7) or quil a alone (control; n=7) and challenged with fasciola hepatica. there were no significant differences in fluke burdens (56+/-26 vs. 92+/-53), average fluke length (20.9+/-3.0 vs. 21.0+/-3.4mm) or faecal egg output (525+/-533 vs. 758+/-677.8 eggs per gram at 17 weeks post-infection) between vaccinated and infected control groups, respectively, but high ...201019665401
fasciola hepatica infections in livestock flock, guanacos and coypus in two wildlife reserves in argentina.between autumn and spring 2006, a coprological survey was performed in two wildlife reserves located in the north of argentine patagonia to determine the prevalence of fasciola hepatica and the number of parasite eggs per gram (epg) of feces in wild guanacos (lama guanicoe), coypus (myocastor coypus), and locally born and raised goats and sheep. snails of the family lymnaeidae were collected in freshwater habitats, identified taxonomically and analyzed parasitologically. prevalence of patent inf ...200919700244
identification and differentiation of fasciola hepatica and fasciola gigantica using a simple pcr-restriction enzyme method.accurate morphological differentiation between the liver fluke species fasciola hepatica and fasciola gigantica is difficult. we evaluated pcr-restriction enzyme profiles of internal transcribed spacer 1 (its1) that could aid in their identification. fifty f. hepatica and 30 f. gigantica specimens were collected from different hosts in three provinces of iran. for dna extraction, we crushed fragments of the worms between two glass slides as a new method to break down the cells. dna from the crus ...201019769969
[cloning, expression and immunogenicity analysis of cathepsin l-like protease of fasciola hepatica].to clone and express the cathepsin l-like protease gene of fasciola hepatica (fhcl) and investigate the immunogenicity of the recombinant fhcl protein.200919852356
immune response of goats immunised with glutathione s-transferase and experimentally challenged with fasciola hepatica.glutathione s-transferase (fhgst) purified from fasciola hepatica adult worms was used to immunise goats against f. hepatica in an experimental infection; the level of protection, in terms of fluke burden, faecal egg counts and hepatic damage was determined, as well as the humoral and cellular immune response elicited. animals were allocated into three groups of six animals each: group 1 (immunised with fhgst and infected), group 2 (unimmunised and infected), and group 3 (unimmunised and uninfec ...201020035898
triclabendazole resistance involving fasciola hepatica in sheep and goats during an outbreak in almirante tamandare, paraná, brazil.fasciolosis is a disease of extreme importance, occurring throughout brazil, with great economic losses to the animal industry. this study aims to determine the effectiveness of treatment against fasciola hepatica on a sheep and goat farm during an outbreak of fasciolosis, in which a high proportion of deaths occurred. the farm harbored 33 sheep and 60 goats of mixed breeds with varying weights and ages. parasite control was based on suppressive, monthly treatments with moxidectin. over the cour ...200820059835
evaluation of hepatic changes and local and systemic immune responses in goats immunized with recombinant peroxiredoxin (prx) and challenged with fasciola against fasciola hepatica in goats immunized with peroxiredoxin (prx) was assessed. the experimental trial consisted of three groups of seven animals; group 1 were unimmunized and uninfected, group 2 were immunized with adjuvant only and group 3 were immunized with recombinant prx in adjuvant (immunized and infected). immunization with prx in quil a adjuvant, group 3, induced a reduction in fluke burden of 33.04% when compared to adjuvant control, group 2, although this difference was ...201020153792
evaluation of hepatic damage and local immune response in goats immunized with native glutathione s-transferase of fasciola hepatica.worm burden, hepatic damage and local cellular and humoral immune responses were assessed in goats immunized with glutathione-s-transferase and challenged with fasciola hepatica. infected but unimmunized and uninfected control groups were also studied. hepatic damage was evaluated grossly and microscopically. local immune response was evaluated by (1) microscopical examination of hepatic lymph nodes (hlns); (2) analysis of the distribution of cd2(+), cd4(+), cd8(+), t-cell receptor gammadelta(+) ...201020185148
[bovine fasciolosis in switzerland: relevance and control].in switzerland, bovine fasciolosis is an economically important but often overlooked disease of dairy cows. the intermediate host of fasciola hepatica in switzerland is galba truncatula, an amphibious snail living in humid habitats which are infected by miracidia from recently hatched fasciola eggs. the definitive hosts include cattle, sheep, goats, horses, and free-living ruminants. infection of these hosts occur from metacercariae, usually encysted on vegetation. infection risk depends on the ...201020464681
gastrointestinal helminthiasis: prevalence and associated determinants in domestic ruminants of district toba tek singh, punjab, pakistan.the objective of the present study was to determine the prevalence and associated determinants (e.g., sex, age, on-farm management and husbandry) of gastrointestinal (gi) helminths in the domestic animals of district toba tek singh, punjab, pakistan. for this purpose, 1,140 cattle, 1,140 buffaloes, 660 goats, 840 sheep, and 156 camels were randomly selected and their fecal samples were screened every other week for a year using a modified floatation technique. the samples positive for strongyle- ...201020532913
improving animal and human health through understanding liver fluke immunology.sheep, goats and cattle represent the most numerous and economically important agricultural species worldwide used as sources for milk, fibre and red meat. in addition, in the developing world, these species often represent the sole asset base for small-holder livestock farmers and cattle/buffaloes often provide the majority of draught power for crop production. production losses caused by helminth diseases of these animals are a major factor in extending the cycle of poverty in developing count ...201020626812
evaluation of local immune response to fasciola hepatica experimental infection in the liver and hepatic lymph nodes of goats immunized with sm14 vaccine against fasciola hepatica in goats immunized with a synthetic recombinant antigen from schistosoma mansoni fatty acid-binding protein 14 (rsm14) was investigated by assessing worm burdens, serum levels of hepatic enzymes, faecal egg count and hepatic damage, which was evaluated using gross and microscopic morphometric observation. the nature of the local immune response was assessed by examining the distribution of cd2+, cd4+, cd8+ and γ´+ t lymphocytes along with igg+, il-4+ and ifn- ...201020835620
transmission dynamics of fasciola hepatica in the plateau region of mexico. effect of weather and treatment of mammals under current farm management.the aim of the present work was to study the dynamics of fasciola hepatica natural infection in ovines, caprines, bovines and two mollusc species, lymnaea (fossaria) humilis and lymnaea (f.) bulimoides, from 2004 to mid 2007 under normal farm management conditions, and the relation to climate changes. the study was performed in a research centre in the plateau of mexico. temperature and rainfall were registered every month, as well as the number and intensity of infection in livestock and mollus ...201021055879
the abattoir condemnation of meat because of parasitic infection, and its economic importance: results of a retrospective study in north-eastern nine districts in the north of khorasan province, in north-eastern iran, a 5-year retrospective study was carried out to determine the prevalences, in livestock slaughtered in abattoirs, of the parasitic infections responsible for the condemnation of the animals' carcasses and viscera (and the economic importance of such infections in terms of lost meat and offal). between 20 march 2005 and 19 march 2010, 436,620 animals (45,360 cattle, 275,439 sheep, 115,674 goats and 147 camels) were slaugh ...201021144183
evaluation of fast-elisa versus standard-elisa to diagnose human fasciolosis.the present study was conducted to evaluate and compare a fast-elisa (f-elisa) method versus standard-elisa (s-elisa) to diagnose human fasciolosis.201121194256
towards delineating functions within the fasciola secreted cathepsin l protease family by integrating in vivo based sub-proteomics and phylogenetics.fasciola hepatica, along with fasciola gigantica, is the causative agent of fasciolosis, a foodborne zoonotic disease affecting grazing animals and humans worldwide. pathology is directly related to the release of parasite proteins that facilitate establishment within the host. the dominant components of these excretory-secretory (es) products are also the most promising vaccine candidates, the cathepsin l (cat l) protease family.201121245911
[human and animal fascioliasis in peru: impact in the economy of endemic zones].fasciola hepatica is the causative agent of fasciolosis in peru; the disease is an important public health problem by the high prevalence of the human infection affecting mainly children and a major veterinary problem by the high rates of infected livestock. the human disease is endemic in the sierra and the coast but sporadic in the amazonia, and reported in 18 departments, while the animal infection in 21 of 24 departments of peru. transmission occurs in andean rural populations engaged in agr ...201021308203
molecular characterization of fasciola spp. from the endemic area of northern iran based on nuclear ribosomal dna sequences.fasciolosis caused by fasciola spp. (platyhelminthes: trematoda: digenea) is considered as the most important helminth infection of ruminants in tropical countries, causing considerable socioeconomic problems. in the endemic regions of the north of iran, fasciola hepatica and fasciola gigantica have been previously characterized on the basis of morphometric differences, but the use of molecular markers is necessary to distinguish exactly between species and intermediate forms. samples from buffa ...201121440546
hybridization experiments indicate incomplete reproductive isolating mechanism between fasciola hepatica and fasciola gigantica.summaryexperiments on hybridization between fasciola hepatica and fasciola gigantica were carried out to clarify whether a reproductive isolating mechanism appears between the two fasciola species. molecular evidence for hybridization was based on the dna sequence of the internal transcribed spacer 1 (its1) region in nuclear ribosomal dna, which differs between the species. the results suggested that there were not pre-mating but post-mating isolating mechanisms between the two species. however, ...201121767436
nematodes and liver fluke in new zealand.abstract a general review of the epidemiology, significance and control of nematode parasitism of sheep, goats, cattle and deer in new zealand, the emergence of anthelmintic resistance and its effects, and the search for parasite control strategies that reduce reliance on anthelmintic use, is provided. the research that has formed the basis for present levels of understanding of this complex and important topic is summarised and sources of further information are indicated. aspects of nematode i ...200221838630
humoral immune response in goats immunised with cathepsin l1, peroxiredoxin and sm14 antigen and experimentally challenged with fasciola hepatica.the humoral immune response was analysed in goats immunised with fhcl1, fhprx, sm14, and experimentally challenged with fasciola hepatica. all immunised animals developed significant levels of anti-fluke specific antibodies and those immunised with fhcl1 showed the highest antibody titre. after experimental infection, an increase in the antibody level was detected only in goats immunised with fhcl1. in the adjuvant-control animals, the experimental challenge induced significant production of spe ...201122001704
gastrointestinal parasitic infection in diverse species of domestic ruminants inhabiting tribal rural areas of southern rajasthan, india.a total of 415 adult domesticated ruminants, 130 cattle (bos taurus), 108 buffaloes (bubalus bubalis), 94 goats (capra hircus) and 83 sheep (ovis aries) inhabiting tribal rural areas of southern rajasthan, india were investigated for evidence of gastrointestinal protozoan and helminthic infections. in southern rajasthan humid ecosystem is predominant and has number of perennial freshwater bodies. fresh faecal samples of these animals were examined microscopically by direct wet smear with saline ...201224431582
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