partial purification and characterization of gigantocotyle explanatum somatic antigens.soluble extracts of gigantocotyle explanatum, isolated from the liver of buffalo bubalus bubalis were fractionated on sephadex g-200 columns. nine major fractions referred to as f1, f2, f3, f4, f5, f6, f7, f8 and f9 were separated. each fraction was tested by elisa for antigenicity using sera from g. explanatum-infected field buffaloes. fractions f1 and f2 were highly antigenic, f3, f4, f6 and f7 were moderately antigenic and f5, f8 and f9 were poorly antigenic. analyses by sds-page revealed tha ...200415153279
quantitative studies on acetylcholinesterase in seven species of digenetic trematodes.quantitative estimation of absolute levels and in vitro release of acetylcholinesterase (ache) in seven species of digenetic trematodes: isoparorchis hypselobagri from the swim bladder of catfish, wallago attu; srivastavaia indica and gastrothylax crumenifer from the rumen, and gigantocotyle explanatum from the liver of the water buffalo, bubalus bubalis; fasciolopsis buski, echinostoma malayanum from the small intestine and gastrodiscoides hominis from the caecum of the pig, sus scrofa reveale ...1977562036
characterization of the glutamate dehydrogenase activity of gigantocotyle explanatum and gastrothylax crumenifer (trematoda: digenea).glutamate dehydrogenase (gldh) (ec is a ubiquitous enzyme, which is present at the protein and carbohydrate metabolism crossroads. the enzyme activity was investigated in biliary and rumen amphistomes, gigantocotyle explanatum and gastrothylax crumenifer, respectively, infecting the indian water buffalo bubalus bubalis. the enzyme activity was consistently higher in g. explanatum as compared to g. crumenifer, where nad(h) was utilized as coenzyme and the ph optima was recorded at 8. the ...200923129890
immunodetection of coproantigens for the diagnosis of amphistomosis in naturally infected indian water buffalo, bubalus bubalis.the infection of gastrointestinal helminths in livestock is routinely diagnosed by microscopical examination of faecal samples for the presence of ova/eggs but this approach becomes ineffective for the seasonally egg producing trematodes. therefore, an alternative approach to detect the coproantigens of liver and rumen amphistomes, gigantocotyle explanatum and gastrothylax crumenifer respectively, infecting indian water buffalo bubalus bubalis, was undertaken using elisa, immunodot and countercu ...201323036808
somatic antigens of tropical liver flukes ameliorate collagen-induced arthritis in wistar rats.parasitic helminths polarize immune response of their vertebrate hosts towards anti-inflammatory th2 type and therefore it is hypothesized that they may suppress the inflammatory conditions in autoimmune disorders. the present study was undertaken to investigate in vivo immunomodulatory and therapeutic potential of somatic antigens (ag) of liver infecting digenetic trematodes [fasciola gigantica (fg) and gigantocotyle explanatum (ge)] in collagen-induced arthritic (cia) wistar rats. the cia rats ...201525992888
2d-page analysis of the soluble proteins of the tropical liver fluke, fasciola gigantica and biliary amphistome, gigantocotyle explanatum, concurrently infecting bubalus bubalis.the digenetic trematodes, fasciola gigantica and gigantocotyle explanatum, belonging to the family fasciolidae and paramphistomidae respectively, have been often found to concurrently infect the liver of indian water buffalo bubalus bubalis, causing serious pathological damage to the vital organ, incurring huge economic losses. in the present study the soluble gene products of both f. gigantica and g. explanatum were analyzed by 2 dimensional polyacrylamide gel electrophoresis. the soluble prote ...201627605808
anthelmintic effect of biocompatible zinc oxide nanoparticles (zno nps) on gigantocotyle explanatum, a neglected parasite of indian water buffalo.helminth parasites of veterinary importance cause huge revenue losses to agrarian economy worldwide. with the emergence of drug resistance against the current formulations, there is a need to focus on the alternative approaches in order to control this menace. in the present study, biocompatible zinc oxide nanoparticles (zno nps) were used to see their in vitro effect on the biliary amphistomes, gigantocotyle explanatum, infecting bubalus bubalis because these nanoparticles are involved in gener ...201526177503
trematode hemoglobins show exceptionally high oxygen affinity.ligand binding studies were made with hemoglobin (hb) isolated from trematode species gastrothylax crumenifer (gc), paramphistomum epiclitum (pe), explanatum explanatum (ee), parasitic worms of water buffalo bubalus bubalis, and isoparorchis hypselobagri (ih) parasitic in the catfish wallago attu. the kinetics of oxygen and carbon monoxide binding show very fast association rates. whereas oxygen can be displaced on a millisecond time scale from human hb at 25 degrees c, the dissociation of oxyge ...19989675199
alkali denaturation of oxyhaemoglobins of some digenetic trematodes and their hosts.oxyhaemoglobins of six digenetic trematodes,--srivastavaia indica, gastrothylax crumenifer, gigantocotyle explanatum, fasciolopsis buski, gastrodiscoides hominis, isoparorchis hypselobagri, and of their 3 vertebrate hosts, bubalus bubalis, sus scrofa, wallago attu, were subjected to alkali denaturation at a ph of 12.4. all oxyhaemoglobins from trematodes and their hosts differ from each other in the rate and extent of alkali denaturation which may be explained due to variations in the amino acid ...1977599278
histochemical and electrophoretic studies on phosphatases of some indian trematodes.the isoenzymes of acid and alkaline phosphatases and their histochemical localization were studied by polyacrylamide disc gel electrophoresis in four species of trematodes: gigantocotyle explanatum from the liver and gastrothylax crumenifer from the rumen of water buffalo (bubalus bubalis) and echinostoma malayanum and fasciolopsis buski from the small intestine of the pig (sus scrofa). both acid and alkaline phosphatases were present in the tegument, gastrodermis, suckers, testes, ovary, eggs, ...19827096966
functional differentiation in trematode hemoglobin isoforms.the hbs and the major electrophoretic hb components (isohbs) were isolated from three species of the trematodes, explanatum explanatum (ee), gastrothylax crumenifer (gc) and paramphistomum epiclitum (pe), that parasitise the common indian water buffalo bubalus bubalis. the hbs are monomeric and resemble the so-called nonfunctional mutant hemoglobins that have tyr at b10 or e7 positions (replacing leu and the his residues, respectively). however, they are capable of binding with o2 and co. o2 equ ...199910103000
lactate dehydrogenase in two digenetic trematodes and their host.polyacrylamide gel electrophoresis of the two digenetic trematodes, gigantocotyle explanatum from the liver and gastrothylax crumenifer from the rumen of the water buffalo, bubalus bubalis revealed the presence of at least six and seven isoenzymes of lactate dehydrogenase (ldh), respectively in a partially purified enzyme preparation. the respective host tissues showed five isoenzymes of ldh, which are characteristic to the vertebrates. both parachloromercuribenzoate and iodoacetate affected the ...19902074142
spectrophotometric analysis of haemoglobins of some digenetic trematodes and their hosts.the haemoglobins of six different species of trematodes: gastrothylax crumenifer, srivastavaia indica, gigantocotyle explanatum, fasciolopsis buski, gastrodiscoides hominis, isoparorchis hypselobagri and their three different hosts: bubalus bubalis, sus scrofa, and wallago attu were spectrophotometrically investigated, and were found to contain porphyrin ix as the common prosthetic group. oxyhaemoglobin, carbonmonoxy-haemoglobin and reduced haemoglobin of all 6 species of trematodes and their 3 ...19761010924
effects of some sugars, amino acids, glycerol and alpha-glycerophosphate on oxygen uptake of some digenetic trematodes.effect of various substrates on the oxygen uptake of some digenetic trematodes -- srivastavaia indica, gastrothylax crumenifer, gigantocotyle explanatum from the buffalo, bubalus bubalis and isoparorchis hypselobagri from the fish wallago attu was studied. in all the four species, the effect of various substrates except maltose was found to be stimulatory. the inhibitory or stimulatory effect of these substrates on oxygen uptake reflects the importance of these compounds in the energy metabolism ...19807450612
studies on the ultrastructure and histochemistry of the lymph system in three species of amphistome (trematoda: digenea) gigantocotyle explanatum, gastrothylax crumenifer and srivastavaia indica from the indian water buffalo bubalus bubalis.the lymph system of three amphistome parasites from buffaloes, gigantocotyle explanatum, gastrothylax crumenifer and srivastavaia indica was studied using light microscope histochemistry and electron microscopy. in each case the system comprised a single pair of main longitudinal vessels which gave rise to numerous sub-dividing lateral branches. although the finer lymph channels associated with most internal systems, they did not penetrate the basement membrane of any organ. the lymph vessels we ...19853989258
non-specific alkaline phosphomonoesterases of eight species of digenetic trematodes.alkaline phosphatases from different trematodes occupying the same habitat have identical ph otima but different levels of enzyme activities. isoparorchis hypselobagri, from the fish wallago attu, shows four to six times more enzyme activity than fasciolopsis buski, gastrodiscoides hominis and echinostoma malayanum, from the pig sus scrofa, and fasciola gigantica, gigantocotyle explanatum, cotylophoron cotylophorum and gastrothylax crumenifer, from the buffalo bubalus bubalis. at least two peaks ...19751442
natural trematode infection in liver of water buffalo (bubalus bubalis): histopathological investigation.this study reports the infection of liver and bile ducts, carried out from november 2008 to april 2010, on 730 randomly selected water buffaloes-bubalusbubalis, infected with the amphistome trematode parasite explanatum explanatum (creplin, 1847) fukui, 1929. macroscopic examination revealed massive infection of adult fluke in bile ducts and intrahepatic ductules in 131 (18%) cases. the predominant features were multifocal granulomatous nodules throughout the luminal surface of the bile ducts. h ...201122654314
Displaying items 1 - 17 of 17