cryptosporidium ryanae n. sp. (apicomplexa: cryptosporidiidae) in cattle (bos taurus).a new species, cryptosporidium ryanae, is described from cattle. oocysts of c. ryanae, previously identified as the cryptosporidium deer-like genotype and recorded as such in genbank (ay587166, eu203216, dq182597, ay741309, and dq871345), are similar to those of cryptosporidium parvum and cryptosporidium bovis but smaller. this genotype has been reported to be prevalent in cattle worldwide. oocysts obtained from a calf for the present study are the smallest cryptosporidium oocysts reported in ma ...200818583057
prevalence and distribution of cryptosporidium spp. in dairy cattle in heilongjiang province, china.few data are available on the molecular characterization of cryptosporidium spp. in cattle in china. in the present study, a total of 507 fecal specimens from six dairy farms in heilongjiang province were examined for cryptosporidium spp. by light microscopy of concentrates from the formalin-ethyl acetate sedimentation method (for less than 2-month-old calves) or sheather's floatation method (more than 3-month-old dairy cattle). twenty-seven post-weaned calves on five farms were positive for cry ...200919424720
a multiplex polymerase chain reaction assay to simultaneously distinguish cryptosporidium species of veterinary and public health concern in cattle.four species of cryptosporidium are routinely found in cattle: cryptosporidium parvum, cryptosporidium bovis, cryptosporidium ryanae, and cryptosporidium andersoni. it is important to determine the species of cryptosporidium in infected cattle because c. parvum is the only serious pathogen for humans as well as cattle. identification of cryptosporidium species and genotypes currently relies on molecular methods such as polymerase chain reaction (pcr) followed by restriction fragment length polym ...200919713046
molecular characterisation of cryptosporidium isolates from swedish dairy cattle in relation to age, diarrhoea and region.cryptosporidium positive samples from 176 preweaned calves, young stock and cows of 48 herds were subjected to molecular characterisation of the 18s rrna gene to determine which species are present in swedish dairy cattle. in addition, samples characterised as cryptosporidium parvum were further analysed at the gp60 gene to investigate distribution and zoonotic potential of subtypes. the 18s rrna gene was successfully sequenced in 110 samples, with cryptosporidium bovis in 83, c. parvum in 15, c ...201020138705
species of cryptosporidium detected in weaned cattle on cow-calf operations in the united determine the species and distribution of cryptosporidium in weaned beef calves in the united states, fecal specimens were collected from 819 cattle between 6 and 18 months of age from 49 operations in 20 states (alabama, california, colorado, georgia, idaho, iowa, kansas, louisiana, mississippi, missouri, nebraska, new mexico, north dakota, oklahoma, oregon, south dakota, tennessee, texas, virginia, and wyoming). fresh feces, collected either from the ground or directly from the rectum of ea ...201020338690
molecular characterization and assessment of zoonotic transmission of cryptosporidium from dairy cattle in west bengal, india.few studies in the past have examined the genetic diversity and zoonotic potential of cryptosporidium in dairy cattle in india. to assess the importance of these animals as a source of human cryptosporidium infections, fecal samples from 180 calves, heifers and adults and 51 farm workers on two dairy farms in west bengal, india were genotyped by pcr-rflp analysis of the 18s rrna gene of cryptosporidium followed by dna sequencing of the pcr products. phylogenetic analysis was carried out on the d ...201020356678
molecular characterization of cryptosporidium spp. in native calves in nigeria.most studies on the distribution of cryptosporidium species in cattle were done with dairy breeds in industrialized nations. in this study, 65 fecal samples from randomly selected 12-24-week-old diarrheic calves in four white fulani herds in southwestern nigeria were screened for cryptosporidium spp. using polymerase chain reaction (pcr)-restriction fragment length polymorphism (rflp) analysis of the small subunit rrna gene. thirty-four (52.3%) of the samples were positive for cryptosporidium. r ...201020644959
cryptosporidium infection in herds with and without calf diarrhoeal problems.a case-control study was designed to investigate the role of different cryptosporidium spp. in swedish dairy herds with and without calf diarrhoeal problems. faecal samples were collected from preweaned calves, young stock and cows. cryptosporidium oocysts were detected by sodium chloride flotation and epifluorescence microscopy. molecular diagnostics were used to identify cryptosporidium species. samples containing c. parvum were further analysed to determine subtypes. calf faecal samples were ...201020714750
the potential for zoonotic transmission of giardia duodenalis and cryptosporidium spp. from beef and dairy cattle in ontario, canada.the objective of this study was to compare the occurrence and the genotypes and species of giardia duodenalis and cryptosporidium spp. in beef and dairy cattle from farms in the regional municipality of waterloo, ontario, in an effort to determine the potential for zoonotic transmission from these animals. pooled manure samples were collected from 45 dairy cattle farms and 30 beef cattle farms. the presence of giardia cysts and cryptosporidium oocysts was determined by immunofluorescence microsc ...201020971563
molecular characterization of cryptosporidium spp. in pre-weaned dairy calves in the czech republic: absence of c. ryanae and management-associated distribution of c. andersoni, c. bovis and c. parvum subtypes.a total of 750 faecal samples of dairy calves at up to 2 months of age kept in various housing systems were screened for cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. dna was extracted from cryptosporidium positive samples and from 150 randomly selected microscopically negative samples. nested pcr was performed to amplify the partial ssu rrna gene of cryptosporidium that was subsequently digested by sspi, vspi and mboii restriction enzymes to determine th ...201021168973
molecular characterization of cryptosporidium spp. in native breeds of cattle in kaduna state, nigeria.despite numerous molecular epidemiologic studies of cryptosporidiosis in dairy cattle in industrialized countries, there are very few studies on the diversity and public health significance of cryptosporidium species in native cattle in developing countries. in this study, a polymerase chain reaction (pcr)-restriction fragment length polymorphism (rflp) analysis of the small-subunit (ssu) rrna gene was used to detect and identify cryptosporidium spp. in 194 fecal specimens from 2 to 365 days old ...201121277091
cryptosporidium infection in non-human hosts in malawi.of 1346 faecal samples from the chikwawa and thyolo districts of malawi, analysed for the presence of cryptosporidium oocysts between october 2001 and may 2003, 61.3% were from cattle (29.8% of these were from calves <6 months old). cryptosporidium oocysts were detected during all three seasons studied in chikwawa and thyolo. in chikwawa, 13.6% of adult cattle and 11.7% of calves were infected, compared to 28.9% of adult cattle and 36.7% of calves in thyolo. dependent on season, between 7.8% and ...200921344786
a whole water catchment approach to investigating the origin and distribution of cryptosporidium species.aims:  investigating the distribution and origin of cryptosporidium species in a water catchment affected by destocking and restocking of livestock due to a foot and mouth disease epidemic. methods and results:  surface water, livestock and wildlife samples were screened for cryptosporidium and oocysts characterised by sequencing ssu rrna and cowp loci, and fragment analysis of ml1, ml2 and gp60 microsatellite loci. oocyst concentrations in water samples (0 to 20.29 per 10l) were related to rain ...201121649804
real-time pcr assay targeting the actin gene for the detection of cryptosporidium parvum in calf fecal samples.cryptosporidium parvum infection is very important with respect to public health, owing to foodborne and waterborne outbreaks and gastrointestinal illness in immunocompetent and immunocompromised persons. in cattle, infection with this species manifests either as a subclinical disease or with diarrheal illness, which occurs more often in the presence of other infectious agents than when alone. the aim of this study was to develop a real-time polymerase chain reaction (pcr) assay for the detectio ...201122042503
common occurrence of a unique cryptosporidium ryanae variant in zebu cattle and water buffaloes in the buffer zone of the chitwan national park, nepal.there are very few studies on the diversity and public health significance of cryptosporidium species in zebu cattle and water buffaloes in developing countries. in this study, pcr-restriction fragment length polymorphism and dna sequence analyses of the small-subunit (ssu) rrna gene were used to genotype cryptosporidium specimens from 12 zebu cattle calves, 16 water buffalo calves, and four swamp deer (cervus duvaucelii) collected from the buffer zone of the chitwan national park, nepal. all cr ...201121996006
occurrence of cryptosporidium and giardia on beef farms and water sources within the vicinity of the farms on prince edward island, canada.the objectives of this study were to determine the prevalence and assemblages of giardia and species of cryptosporidium on beef farms in prince edward island (pei), canada, including the water sources associated with the farms, and to determine risk factors for infection of cattle with these parasites. twenty beef farms were selected based on the presence of surface water<500m from the barn. prevalence was determined by direct immunofluorescence microscopy, while genotyping and species determina ...201122112978
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