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intestinal trematodes infecting humans in korea.sixteen species of intestinal trematodes have been recovered from humans in korea. they include 10 species of the family heterophyidae (metagonimus yokogawai, m. takahashii, heterophyes nocens, h. heterophyes, h. dispar, heterophyopsis continua, pygidiopsis summa, stellantchasmus falcatus, centrocestus armatus, and stictodora fuscatum); one species of diplostomidae (fibricola seoulensis); three species of echinostomatidae (echinostoma hortense, e. cinetorchis, and echinochasmus japonicus); one s ...19911822879
echinostoma revolutum and echinoparyphium recurvatum recovered from house rats in yangyang-gun, kangwon-do.during an investigation on intestinal flukes of house rats in yangyang-gun, kangwon-do, a total of 6 species of trematodes belonging to 3 families; echinostomatidae (echinostoma hortense, e. cinetorchis, e. revolutum and echinoparyphium recurvatum), diplostomidae (fibricola seoulensis) and plagiorchiidae (plagiorchis muris), were recovered from two adult rats. e. revolutum and e. recurvatum were new trematode faunae of rats in korea. e. revolutum had an elongated body, 5.3-6.0 mm long and 1.0-1. ...19902099177
histochemical findings of the tribocytic organ and tegument of fibricola seoulensis.the tribocytic organ and tegument of fibricola seoulensis were examined histochemically for the detection of carbohydrates, mucosubstances, amyloid, collagen and alkaline phosphatase. the surface, secretes, gland cells of the tribocytic organ, and the tegument of the worms were positive to periodic acid schiff (pas) and pas with diastase stain but negative to other stains. it was inferred that the tribocytic organ and tegument of f. seoulensis comprise neutral mucopolysaccharides, which may take ...19902151308
comparative growth and development of the metacercariae of fibricola seoulensis (trematoda: diplostomidae) in vitro, in vivo and on the chick chorioallantois.the growth and development of the metacercariae of f. seoulensis cultivated in vitro or on the chick chorioallantois were assessed by comparison with the optimum process of maturation in albino rats and new born chickens. the process of maturation was divided for convenience into six stages: stage 1; cell multiplication, stage 2; body shaping, stage 3; separation of genital anlagen, stage 4; organogeny, stage 5; gametogony, and stage 6; oviposition. in hank's and tyrode's solutions, the metacerc ...19892486835
[observation of mucosal pathology after praziquantel treatment in experimental fibricola seoulensis infection in rats].it is well known that the duodenum of mice or rats infected with fibricola seoulensis shows atrophy of villi (shortening, blunting, widening, fusion) and hyperplasia of crypts. this study was performed to observe healing process of these pathological changes after deworming with anthelmintic treatment. albino rats infected each with 1,000 metacercariae of f. seoulensis were treated with single dose of 10 mg/kg praziquantel on day 15 post-infection. on day 1, 3, 5, 7, 15, 21 and 28 after the trea ...19892487261
neodiplostomum seoulensis n. comb. (trematoda: neodiplostomidae).fibricola seoulensis seo, rim, and lee, 1964 was described from rats in seoul, korea and was later found to be a natural parasite in the intestine of humans. at the time this species was described, the classification of this and other strigeiform flukes was based largely on the phylogeny of the host; those adult worms parasitic in birds were placed in neodiplostomum and those parasitic in mammals were placed in fibricola. a recent analysis of this group produced a classification that was based o ...19948064539
[transmission electron microscopic ultrastructures of the male germinal cells of fibricola seoulensis].a transmission electron microscopic study was performed to observe the ultrastructures of the male germinal cells and spermatozoa of fibricola seoulensis. spermatogonia were found in the periphery of the testis and characterized by large nuclei and comparatively little cytoplasms. spermatocytes contained an oval to spherical nucleus. their nuclear volume was little larger in comparative to that of cytoplasm, and the chromatin was comparatively little. the early spermatids were characterized by a ...19938241077
[transmission electron microscopic ultrastructure of the tegument of fibricola seoulensis].an electron microscopic study was performed to observe the ultrastructure of the tegument of f. seoulensis. the outer surface of the tegument was covered with a trilaminated plasma membrane. the electron-dense cytoplasmic layer was 2.5 microns wide in the anterior portion and contained numerous vacuoles, mitochondria and granular materials in its matrix. the basement layer was 330 nm wide or so, and its numerous extensions protruded into the cytoplasmic layer. the sensory organ was composed of a ...19938297887
transmission electron microscopic findings of the tribocytic organ of fibricola seoulensis.we observed ultra-structure of the tribocytic organ of fibricola seoulensis with transmission electron microscope. microvilli are observed on the surface of the tribocytic organ. below the muscle layer, we can find three types of cells. type i cell has electron lucent cytoplasm with a few granules, while type ii cell shows electron dense cytoplasm and the particulate granules. type iii cell's electron dense cytoplasm possesses many granules. of the above three cells, type i and ii cells are beli ...19938297888
[the nervous system of fibricola seoulensis by acetylcholinesterase histochemistry].f. seoulensis were obtained from artificially infected albino rats at 3, 4, 5, 6, 7 days after infection. the worms and metacercariae were washed in physiological saline solution, and fixed with 10% neutral formalin. the acetylcholinesterase (ache) stained by enzyme histochemistry using acetylthiocholine iodide as substrate. eserine, iso-ompa and bw284c51 were used as inhibitors of ache. the nervous system consists of three pairs longitudinal nerve trunks interconnected with excretory plexus in ...19938297889
[localization and isozyme patterns of phosphatase in fibricola seoulensis].the present study was carried out to investigate the localization and isozyme patterns of acid phosphatase and alkaline phosphatase in metacercariae and in adults of f. seoulensis by enzyme-histochemistry method and electrophoresis. acid phosphatase showed a strong activity at ph 5 in the intestinal caecum of adults, but showed no reactions in the non-substrate control and in the inhibitor-treated control. alkaline phosphatase showed a strong activity at ph 8 in the intestinal caecum and the tri ...19938297893
[worm recovery rate and small intestinal lesions of albino rats coinfected with fibricola seoulensis and metagonimus yokogawai].worm recovery rates and pathologic changes in small intestine of albino rats were observed after concurrent and challenge infections with metacercariae (mc) of fibricola seoulensis and metagonimus yokogawai, and compared with those of single infection groups. albino rats in concurrent infection group were killed 20 days after feeding with 1,000 mc of each fluke. rats in challenge infection group were fed with 1,000 mc of f. seoulensis and challenged by 1,000 mc of m. yokogawai 10 days after prim ...19938343452
activities of acid phosphatase and non-specific esterase are present in the tribocytic organ and the caecum of fibricola seoulensis.in order to know the enzyme activities of fibricola seoulensis, an intestinal trematode of human and rodent in korea, the enzyme histochemical method is applied. activities of acid phosphatase (e.c.3.1.3.2) and non-specific esterase (e.c.3.1.1) were present in microvilli and glandular cells of tribocytic organ and the epithelium of the caecum.19938343459
clinical and histopathological findings in mice heavily infected with fibricola seoulensis.to observe the clinical course and intestinal histopathology in heavy infection of fibricola seoulensis, an experimental study was performed in mice. clinical, hematological and histopathological observation was done on 1, 3, 7 and 12 days after experimental infection with l,000 metacercariae. on the 11th day after infection, the mice began to die and all of the infected mice were dead by the 16th day. the infected mice showed gradual weight loss. occult blood was detected after the 10th day. di ...198812811032
the life cycle and larval development of fibricola seoulensis (trematoda: diplostomatidae).the life cycle of fibricola seoulensis was studied in the laboratory and in the field, with special interests in the larval developments within the eggs and in the intermediate hosts. the first emergence of miracidia after incubation of eggs in 26c water began on the ninth day. the miracidia, elongate and cylindrical shape, had epidermal plates in the formula of 6, 9, 4 and 3, with two pairs of flame cells and lateral processes. a kind of fresh water snail, hippeutis (h.) cantori, was found to s ...198812811046
egg laying capacity of fibricola seoulensis in mice and rats.the egg laying capacity of fibricola seoulensis was observed in mice and rats. in mice, the mean number of eggs produced by an adult worm a day was 116 at the first week after infection, 123 at the 2nd week and 42 at the 3rd week. thereafter, the capacity of about 20 eggs/worm/day was maintained up to one year period. in rats, it generally produced more eggs; 49 at 1 week, 250 at 2 weeks, 216 at 4 weeks, 327 at 6 months and 11 at one year after infection. it survived longer than a year in mice a ...198812811057
growth and development of fibricola seoulensis metacercariae in tadpoles.in order to observe the growth and development of fibricola seoulensis metacercariae, the tadpoles of rana nigromagulata were experimentally infected with the cercariae. the metacercariae of various developmental stages were recovered from the tadpoles after 2 to 65 days of infection. they were prepared for morphological observation, and were given orally to mice to observe their infectivity. the following results were obtained. all of the tadpoles exposed to the cercariae were observed to harbo ...198612886087
the effect of temperature and salinity on maturation and hatching of fibricola seoulensis eggs.this study was carried out to reveal the effect of temperature, salinity and aeration on maturation and hatching of fibricola seoulensis eggs. the eggs were incubated and were observed daily for the appearance of eyespots and hatching. the results were summerized as follows: from 4-5 days after incubation in distilled water at 28c or at 11-26c, the eyespots began to appear and the rate of eggs with eyespots were over 90% in 28c on the 7th or 8th day. however, eyespots did not appear in 5-15c or ...198612886088
[a survey on intestinal parasites of soldiers in korea]total of 2,643 korean soldiers were examined of their stool for parasitic infections by both cellophane thick smear and formalin-ether concentration techniques from august 1983 to december 1985. out of them, 73.6% were free from any parasite, 22.6% were ova positive and 4.0% cyst positive. the ova positive rates by species were ascaris lumbricoides 2.0%, trichuris trichiura 13.0%, hookworm and trichostrongylus orientalis 0.08% respectively, clonorchis sinensis 7.6%, metagonimus yokogawai 1.1%, p ...198612886100
ten human cases of fibricola seoulensis infection and mixed one with stellantchasmus and metagonimus.ten soldiers of the korean army were found to have f. seoulensis ova in their faeces. these men were treated with praziquantel and the worms passed after purging were successfully collected in one case: 59 f. seoulensis, 68 metagonimus and 2 stellantchasmus falcatus were collected. 2 men were also infected with clonorchis sinensis. all the cases of fibricoliasis had eaten raw or undercooked snake meat or frogs during survival training. all were egg-negative 2-6 months after treatment.198612886114
studies on intestinal trematodes in korea: xviii. effects of praziquantel in the treatment of fibricola seoulensis infection in albino rats.the therapeutic effect of praziquantel on fibricola seoulensis infection were studied by experimental infection of albino rats with the metacercariae obtained from the snakes. total 51 albino rats were infected each with 500 or 1,000 metacercariae in number through intragastric tubes. one or four weeks later the rats were treated with 1, 5, 10 or 20 mg/kg sigle dose of praziquantel and sacrificed after 3 days or 10-120 minutes to search for the worms in their small intestine. the worms recovery ...198512888685
studies on intestinal trematodes in korea xix. light and scanning electron microscopy of fibricola seoulensis collected from albino rats treated with praziquantel.an experimental study was performed to observe the in vivo effects of praziquantel on the light and scanning electron microscopic morphology of fibricola seoulensis. the metacercariae were obtained from the snakes and 1,000 in each number was orally given to total 15 albino rats; 5 controls and 10 treatment group. seven days later the 10 rats were treated with 10 mg/kg praziquantel and sacrificed 1~24 hours later to search for the worms from their small intestines. the major light microscopic ch ...198512888686
a histopathological study on the intestine of mice and rats experimentally infected by fibricola seoulensis.a histopathological study was carried out on the duodenum of mice and rats experimentally infected by f. seoulensis. each mouse was infected wit 500 metacercariae and killed after 1, 2, 3 days, 1 and 2 weeks from infection. each rat was given 1,000 metacercariae and was examined after 1, 2, and 4 weeks from infection. the duodenal tissue sections of mice and rats were stained with hematoxylin and eosin, and pas stained for the rats of 1 week group. the pathological findings are summarized as bel ...198512888687
[a study on the infection status of tadpoles and frogs by the metacercariae of fibricola seoulensis in korea]a total of 220 tadpoles was captured in 6 areas and total 117 frogs, rana nigromaculata, were collected in 11 areas in korea. they were examined for their infecion status by the metacercariae of fibricola seoulensis by peptic digestion technique and by histological observation with hematoxylin-eosin staining. this study was carried out from august 1983 to september 1984. followings are the results. the tadpoles of r. nigromaculata were positive for the metacercariae from 3.3 percent to 100 perce ...198512888688
[studies on intestinal trematodes in korea x. scanning electron microscopic observation on the tegument of fibricola seoulensis]a scanning electron microscopic study was performed to observe the tegumental surface of adult fibricola seoulensis. the adult worms were collected from the small intestine of mice 5 days to 3 weeks after experimental infection with the metacercariae. the metacercariae were obtained from the viscera of the snakes, natrix tigrina lateralis, by artificial digestion technique. the results were as follows: the tegument of anterior body was covered with cobblestone-like cytoplasmic processes and that ...198412891027
fifteen human cases of fibricola seoulensis infection in korea.a mass fecal examination was performed in seoul, korea from december 1983 to march 1984 and 15 egg positive cases of fibricola seoulensis were discovered. they were treated with praziquantel(distocide(r)) 20 mg/kg single dose and magnesium salt for purgation. the adult worms of f. seoulensis were collected from 6 of them. the number of the flukes ranged 1-78 by the case. the cases had no clinical mainfestation at all. they were all korean males in twenties, and had the histories of eating the ro ...198412891032
studies on intestinal trematodes in korea: ix. recovery rate and development of fibricola seoulensis in experimental animals.an experimental study was carried out to observe the susceptibility of several kinds of laboratory animals to fibricola seoulensis infection, a diplostomatid fluke of mammals. the metacercariae were obtained from the viscera of the snakes, natrix tigrina lateralis and 50-2,000 in number each was artificially fed to a total of 127 animals; albino rats, mice, dogs, cats, rabbits and chickens. after 3 days to 8 weeks the animals were sacrificed and the recovery rate of worms as well as their maturi ...198312902653
studies on intestinal trematodes in korea: v. a human case infected by fibricola seoulensis (trematoda: diplostomatidae).a 25-year old korean male was admitted to seoul national university hospital on july 5, 1982 because of fever, epigastric discomfort and diarrhea. laboratory examination revealed moderate eosinophilia, and large operculated helminth eggs in stool repeatedly. other laboratory test resulted in normal range and bacteriological cultures were nefative. under the impression of intestinal fluke infection, bithionol treatment with magnesium purgation was tried. from the diarrheal stool after treatment, ...198212902681
studies on intestinal trematodes in korea: vi. on the metacercaria and the second intermediate host of fibricola seoulensis.this study was carried out to confirm the infection source of the human case of fibricola seoulensis, and to reveal out a part of its life cycle in korea. also the morphological characteristics of the metacercaria were described. the results were summarized as follows: 1. rana nigromaculata and natrix tigrina lateralis were found to be infected naturally by the metacercariae(diplostomula) of f. seoulensis. the metacercarial capsule was round to elliptical in tissue of the intermediate hosts with ...198212902682
studies on intestinal trematodes in korea: vii. growth, development and recovery of fibricola seoulensis from experimentally infected rats and mice.this study was carried out to observe the growth and development of fibricola seoulensis in experimentally infected rats and mice. the metacercariae were obtained from naturally infected natrix tigrina lateralis which were collected in hoengseong gun, gangweon do, in august 1982. the results were summarized as follows. 1. growth and development of worm were not different between the rat and mouse. body growth was attained within a week in both hosts. the testes differentiated first on the 2nd da ...198212902683
studies on parasitic helminths of korea 5.survey on intestinal trematodes of house rats.a study was carried out to evaluate the house rats in southern korea as reservoir host of intestinal trematodes, in 13 different areas: 7 in inland and 6 in brackish-water zones, during the period from august 1980 to august 1981. a total of 170 house rats was captured; 101 rats from inland and 69 from brackish-water zones. they consisted of 129 rattus norvegicus, 22 r. rattus rufescens, and 19 unidentified. the infection status of the rats were as follows: 1. total 29 rats (17.1%) were infected ...198112902707
studies on the parasitic helmiths of korea: i. trematodes of rodents.a total of 624 rodent of six species was examined for trematodes. five species of trematodes were considered, of which fibricola seoulensis sp. nov. was reported as a new species. plagiorchis muris, echinostoma cinetorchis and euparyphium murinum was recorded for the first time from korea. descriptions are included for each species considered.196412913605
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