application of the indirect fluorescent antibody test for diagnosis of theileria hirci infection of sheep: using cell culture schizont antigen.the indirect fluorescent antibody test was applied for detection of circulating antibodies in sheep as a result of theileria hirci infections. a schizont antigen was prepared from an in vitro culture suspension of lymphoid cells infected with t. hirci macroschizonts. the peak antibody titre of 1/8, 192 was reached 24 days after the initial antibody rise in the sheep experimentally infected by means of hyalomma anatolicum anatolicum ticks.1976820024
the use of tissue culture attenuated live vaccine for theileria hirci.malignant sheep theileriosis which is a tick-borne disease is caused by the protozoan parasite theileria hirci. the pathogenic form of the parasite, schizonts, inhabits the lymphoid cells of the mammalian host, and if an immunity is engendered against them animals can resist the infection. theileria hirci infected lymphoid cells were cultivated in vitro, using eagle's mem supplemented with 10% ovine serum, yeast extract and lactalbumine hydrolysate (yl), respectively 250 and 1250 mg/l. schizonts ...19853938739
vectors of theileria hirci in south and southeast of iran. 19705498976
immunization of sheep against theileria hirci infection with schizonts propagated in tissue culture.two experiments were carried out. in the first, three groups of lambs were inoculated subcutaneously with 3 x 10(6) schizonts of different passages (3, 30 and 63) of theileria hirci propagated in tissue culture. severe reactions were observed in lambs inoculated with organisms derived from the 3rd passage. in the second experiment, four groups were inoculated with 5 x 10(5), 3 x 10(6), 1 x 10(7) and 5 x 10(8) schizonts of the 63rd passage. no clinical reactions or parasites were detected in lamb ...19816806971
some characteristics of ovine lymphoid cells infected in vivo by theileria hirci.a panel of monoclonal antibodies reacting with different subsets of sheep lymphocytes plus a polyclonal rabbit anti-sheep ig antiserum were used to characterize three theileria hirci-infected lymphoid cell lines that had been infected and transformed in vivo. in parallel, peripheral blood mononuclear cells of swedish landrace sheep were exposed to the same antibodies. whereas the values obtained for healthy swedish landrace sheep peripheral mononuclear cells fell within the range previously desc ...19938493242
the indirect fluorescent antibody test based on schizont antigen for study of the sheep parasite theileria indirect fluorescent antibody test (ifat), based on schizont-infected lymphoblastoid cells, was applied to study the course of antibody production in adult sheep inoculated with attenuated, in vitro grown, theileria lestoquardi (theileria hirci) infected cells. bright fluorescence of the intracellular schizonts could first be demonstrated 15 days after inoculation. a 32-64-fold rise in antibody titres was recorded 1 month after infection, and substantial titres were still observed 90 days aft ...19979187025
tick-borne diseases of sheep and goats caused by babesia, theileria or anaplasma spp.a review is given on the babesia, theileria, and anaplasma species infecting sheep and goats. b. ovis is the most important disease agent. it is transmitted by rhipicephalus bursa, r. turanicus, hyalomma anatolicum excavatum, and probably by r. evertsi evertsi b. ovis is widely spread in southern europe, the middle east, and central asia. its geographical distribution in south and east asia and in africa is widely unknown. b. motasi obviously represents several nosodemes in separate regions. it ...19979530692
tick-borne diseases of sheep and goats and their related vectors in iran.haemoparasitic diseases have long been considered as a major problem to efficient sheep and goats production in iran. theileriosis due to theileria hirci and babesiosis due to babesia ovis and b. motasi are the most pathogenic protozoa. b. crassa, anaplasma ovis and eperythrozoon ovis are usually non-pathogenic and do not cause any apparent problem. the major tick genera found on sheep and goats are hyalomma, rhipicephalus, haemaphysalis, ixodes and rarely dermacentor distributed in all part of ...19979530694
conventional vaccines for tick-borne haemoparasitic diseases of sheep and goats.of the many tick-borne haemoparasites of sheep and goats, three may be controlled by vaccination. a live virulent blood vaccine for cowdria ruminantium infection (heartwater) is used widely in southern africa in an infection and treatment procedure. a live vaccine for babesia ovis attenuated by passage in splenectomised sheep has been used extensively in bulgaria with good results. a live vaccine for theileria hirci, consisting of schizonts propagated in a lymphoid cell culture and attenuated by ...19979530695
status of the tick-borne diseases in sheep and goats in turkey.a serological survey using indirect fluorescent antibody test (ifat) for babesia ovis infection of sheep has been carried out in different geographical regions of turkey. the results indicated that 71.6% of 141 sheep in black sea region, 70.9% of 93 sheep in central anatolia, 80.2% of 96 sheep in aegean region and 55.7% of 122 sheep in eastern anatolia were seropositive. this means that b. ovis infection is endemic throughout turkey. in addition, occurrence of b. ovis, b. motasi, theileria hirci ...19979530701
phylogenetic analysis by rrna comparison of the highly pathogenic sheep-infecting parasites theileria lestoquardi and a theileria species identified in the northwestern part of china there have been reports of clinical cases in small ruminants of a haemoparasite with the characteristics of theileria hirci (t. lestoquardi). however, some properties of this parasites argue against its classification as t. lestoquardi. in this paper, we present evidence that t. lestoquardi and the chinese theileria isolate are distinct parasite species. phylogenetic analysis of determined nucleotide sequences of small subunit ribosomal rna (srrna) genes of t. l ...200011193632
effect of peganum harmala (wild rue) extract on experimental ovine malignant theileriosis: pathological and parasitological findings.malignant theileriosis of sheep is a highly fatal, acute or subacute disease is caused by the tick-borne protozoan parasite, theileria hirci. in this investigation ten healthy male lambs aged 5-6 months were randomly divided into two groups, a and b and were kept in isolated tick-proof pens. they were treated for internal and external parasite before commencement of the experiment. the lambs were experimentally infected with t. hirci by placing ticks hyalomma anatolicum anatolicum infected with ...200818575066
hyalomma impeltatum (acari: ixodidae) as a potential vector of malignant theileriosis in sheep in saudi arabia.little is known about tick-borne diseases in saudi arabia, particularly regarding the prevalence of theileriosis in small ruminants. this survey studied the potential vectors of malignant theileriosis in saudi arabian sheep. blood, lymph node and tick samples were collected from animals being treated or necropsied at the veterinary diagnostic laboratory in jeddah, makkah (western region), and bureida, al-qasim (central region). blood and lymph node smears were prepared and examined for theileria ...200111511417
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