| identification of simbu, california and bunyamwera serogroup bunyaviruses by nested rt-pcr. | we describe a reverse transcription-polymerase chain reaction (rt-pcr) with primers that anneal to the 5' and 3' ends and amplify the bunyavirus s rna segments. the rt-pcr was done on the fluids of c6/36 cells infected with each of 21 bunyaviruses. the bunyaviruses studied, with the exception of catu virus, produced amplicons having 700 to 1300 base pairs and probably contained the whole s rna segment sequence. a nested pcr performed with these amplicons distinguished california and most bunyamw ... | 2001 | 11280054 |