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ecdysone oxidase, an enzyme from the blowfly calliphora erythrocephala (meigen).in the blowfly, the formation of 3-dehydroecdysone from the insect molting hormone ecdysone is catalyzed by an enzyme which carries hydrogen from ecdysone and ecdysterone to oxygen. the enzyme is therefore called "ecdysone oxidase". two methods are described for the detection of ecdysone oxidase activity, one using a radiolabelled substrate which is separated from the product by thin-layer chromatography after the reaction, and the other using dichloroindophenol, which is discoloured by the redo ...1975297
water transport across peritrophic membranes of calliphora erythrocephala--vii. 19767407
the effect of prostaglandin e1 on cyclic amp production in the salivary glands of calliphora erythrocephala. 1977198235
[acid phosphatase activity in the hemolymph of the fly calliphora erythrocephala during metamorphosis].acid phosphatase is present in the haemolymph of the fly calliphora erythrocephala. the activity of this enzyme has been studied during development; it shows a marked increase during hours following pupation. this increase of activity may be related to the histolytic processes which are acting during metamorphosis.1976139998
changes in acid mucopolysaccharides during the development of the blowfly, calliphora erythrocephala. 1976133191
the comparative biochemistry of invertebrate mucopolysaccharides-v. insecta (calliphora erythrocephala). 1976129315
transport of glial cell acid phosphatase by endoplasmic reticulum into damaged axons.earlier observations indicated that cutting the photoreceptor (r) cell perikarya in the retina of the flies musca domestica and calliphora erythrocephala resulted in an extremely rapid degeneration of the r-cell axons. the process manifested itself within minutes and secondary lysosomes appeared early on in the degenerating axons. in this study, biochemical assays of the classical lysosomal marker enzyme acid phosphatase (acpase) showed a rapid increase in activity upon cutting the retina, reach ...1979457814
the atpase system in the compound eye of the blowfly, calliphora erythrocephala (meig). 1975240606
chromomeres and puffing in experimentally induced polytene chromosomes of calliphora erythrocephala.in the most advanced types of meroistic ovaries the synthesis of rna for the growing oocyte in each follicle is taken over by nurse cells, i.e., sister cells of the definite egg cell. in calliphora, the highly polyploid nurse cells (nc) develop a polytene karyotype under conditions of strict brother-sister inbreeding and using a controlled selection technique. a comparison of the polytene nc-chromosomes with those from the pupal bristle forming cells reveals an unexpected discrepancy: while both ...1979510083
synthesis and turnover of hdrna and mrna in the salivary gland of the blowfly calliphora erythrocephala. 1979437351
[ultrastructure of the mid-intestine muscle of the larva of calliphora erythrocephala meig].the fine structure of the mid-gut muscle in larval calliphora erythrocephala meig. electron microscopy reveals and mid-gut muscle fibres to be composed of an array of actin and myosin myofilaments. the perforated z-band divides the fibril to sarcomeres. the average length of the sarcomere in 5,34 micrometer for the non contracted muscle and 2,09 micrometer for the fully contracted muscle. the thick filaments of the fully contracted muscle pass through the spaces of the z-structures.1979441456
the presence of a hypoglucemic and hypotrehalocemic hormone in the neurosecretory system of the blowfly calliphora erythrocephala. 1978569615
the abdominal epidermis of the blowfly calliphora erythrocephala. ii. imaginal histoblasts during larval life.abdominal histoblasts in calliphora (diptera) are situated within the larval epidermis, and secrete larval cuticle throughout the 3 larval instars. an optical and electron-microscopic study of histoblasts and epidermal sensilla suggests that the latter have been previously confused with histoblast anlagen in the first instar. a steady rate of division until the mid-third instar leads to anlagen growth, which is not seen in larval drosophila. after a relatively quiescent period, division begins a ...1977599177
the effect of chromosomal polytenization on the rates of rna synthesis and decay in the salivary glands of the blowfly, calliphora erythrocephala. 1978620873
[morphogenesis of the imaginal abdominal epidermis in calliphora erythrocephala (insecta,diptera)].the morphogenesis of the abdominal epidermis in calliphora erythrocephala begins by a cellular proliferation which proceeds slowly in larvae and rapidly in pupae. this allows histoblasts to glide and invade the whole abdominal surface. as soon as the new epidermal sheet has become continuous, differentiation begins. generalized epidermal cells show an intense activity which leads to the deposition of imaginal cuticle from the 6th day after pupation onwards. after cuticle deposition they darken a ...1978632740
ontogenic fate of cerebral neurosecretory cells in calliphora erythrocephala (meig.) (cyclorhapha: diptera). 1978640538
the differential regulation of the synthesis of ribosomal rna, 5 s rna, and 4 s rna in the polytenic salivary gland cells of the blowfly, calliphora erythrocephala. 1978680366
production of specific-protein secretion granules by fat body cells of the blowfly, calliphora erythrocephala. substitution of an ovarian key factor by beta-ecdysone.during the first four days of the imaginal stage the fat cells of ovariectomized females of calliphora develop a protein synthetic apparatus, and produce dense bodies (lysosomes) as do the fat cells of normal females, but apparently they cannot synthesize the protein secretion granules that characterize the productive phase of the fat cells of normal females and that we believe to represent vitellogenin. injection of ovariectomized females with beta-ecdysone restored the ability of the fat cells ...1978719713
normal and experimentally induced lysosomal activity in the larval fat body of calliphora erythrocephala meigen.acid phosphatase activity was demonstrated by em-cytochemistry in 4 day old third instar larvae of the fly calliphora erythrocephala meigen, but not in younger stages. during larval development, the activity increased, reaching a maximum at the onset of pupariation. the reaction product was localized in golgi vesicles and sacculi, in vacuoles and in protein granules of varying size and composition, confirming the autophagic character of the protein granules. throughout larval development, the re ...1979519734
demonstration of acid phosphatase activity induced by 20-hydroxyecdysone in the fat body of calliphora.in the larval fat body of calliphora erythrocephala, protein accumulation and autophagic activity occur prior to the onset of puparium formation. the involvement of the lysosomal system in the degradation of sequestered protein and cell organelles can be demonstrated by the electron-microscopical cytochemical localization of the lysosomal marker enzyme acid phosphatase in so-called protein granules. these granules contain not only newly synthetized or absorbed protein but also remnants of cell o ...1979487409
fixation-sensitive areas in the eyes of the walking fly, calliphora erythrocephala.in calliphora erythrocephala the visual fixation behaviour in one-eyed flies and partial blinded flies has been investigated. one-eyed flies show approximately the same stripe and edge fixation response as intact flies. elimination of the frontal eye parts including the binocular field of vision does not effect the visual stripe fixation. on the other hand, if only the frontal areas of both eyes including the binocular field of vision are left open, no preferential direction can be observed (fig ...1978728495
[the in vivo synthesis of ribonucleic acid in the imaginal wing discs during calliphora erythrocephala development]. 1975808282
the hydroxylation of tyrosine by an enzyme from third-instar larvae of the blowfly calliphora erythrocephala.1. two pro-(phenol oxidase) were distinguished when the blood of late-third-instar larvae of calliphora erythrocephala was electrophoresed in polyacrylamide gels with tris-glycine buffer, ph 8.3. one pro-(phenol oxidase), after activation by an enzyme readily catalyses the oxidation of both l-tyrosine and l-3,4-dihydroxyphenylalanine (l-dopa). the second enzyme catalyses the oxidation of l-dopa but not of l-tyrosoine. 2. one of the pro-(phenol oxidases) was purified over 2000-fold from homogenat ...1975810140
[degeneration of the brush border in the midgut of calliphora erythrocephala meig].the brush border of the intestinal epithelium is described in an actively feeding larvae of calliphora erythrocephala meig. it will persist intact after the appearance of the first signs of degeneration of the larval intestinal cells. it will then be invaginated inside the cell forming a "microvillar dense body."1978734198
an ultrastructural study of the in vitro phagocytosis of escherichia coli by the hemocytes of calliphora erythrocephala. 1976775121
chloramphenicol and ethidium bromide effects on flight-muscle mitochondria of the blowfly calliphora erythrocephala. 1978744361
pattern and polarity of sclerites in adult abdominal segments of calliphora erythrocephala (diptera): anlage rotation experiments.the anlagen of imaginal histoblasts in the abdominal segments of calliphora (higher diptera) present an interesting problem, which bears on recent concepts employed in the consideration of spatial patterning in insects. they differ from imaginal discs with respect to larval organization and activity, and in the absence of the progressive pattern of genetic determination during the larval period, characteristic of imaginal discs. how is the adult pattern in the abdominal segments determined? the ...1977870595
the isolation of an o-diphenal oxidase from third-instar larvae of the blowfly calliphora erythrocephala.1. the isolation of an o-diphenol oxidase from an acetone-dried powder of late-third-instar larvae of calliphora erythrocephala was investigated. an insoluble and micro-crystalline fraction containing the enzyme activity was obtained after fractionating extracts of the acetone-dried powder with (nh4)2so4 and acetone. 2. this fraction can be solubilized in 0.1% sodium dodecyl sulphate without loss of activity. 3. polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate shows ...1975812490
intracellular responses from cells of the medulla of the fly, calliphora erythrocephala. 1976953083
effects of weismann ring gland on rna synthesis in imaginal wing disks of calliphora erythrocephala. 1976965778
ultrastructure of the myocardial cell and its membrane systems in the adult fly calliphora erythrocephala (insecta: diptera).calliphora erythrocephala has cross-striated cardiac muscle cells with a, i and z-bands. the diameters of the myosin and actin filaments are 200-250 a and 85 a respectively and the length of the myosin filaments (a-band) is approximately 1.5 micron. usually 8-10 actin filaments surround each myosin filament. the myocardial cells show a well-developed membrane system and interior couplings. a perforated sheet of sr envelopes the myofibrils at the a-band, dilates into flattened cisternae at both a ...1977872197
how far does exposure to radiation mimic ageing in insects?--i. life table data from the blowfly, calliphora erythrocephala. 1979520433
excretion of alkaloids by malpighian tubules of insects.nicotine is transported at high rates by malpighian tubules of larvae of manduca sexta, pieris brassicae and rhodnius prolixus and the transport persists in the absence of alkaloid from the diet. in the fluid-secreting portion of rhodnius tubules this transport is not coupled to ion transport, nor is it dependent on the physiological state of the animal. the transport, which can occur against a steep electrochemical gradient, shows saturation kinetics with a maximal rate of 700 pmol. min-1 per t ...1976932618
in vitro synthesis of peritrophic membranes of the blowfly, calliphora erythrocephala. 19751159308
a family of quasi-white random signals and its optimal use in biological system identification. part ii: application to the photoreceptor of calliphora erythrocephala. 1977889958
mode of formulation of cholesta-5,7-dien-3beta-ol from cholest-5-en-3beta-ol by larvae of calliphora erythrocephala.1. the conversion of cholest-5-en-3beta-ol (cholesterol) into cholesta-5,7-dien-3beta-ol by axenic calliphora erythrocephala larvae was demonstrated. 2. the transformation is probably direct (delta(5)-->delta(5,7)) and does not involve a delta(0) intermediate (delta(5)-->delta(0)-->delta(7)--> delta(5,7)). 3. delta(7)-bond formation involves the stereospecific elimination of the 7beta hydrogen atom. 4. the relative amounts of free and esterified sterols were determined in larvae grown on cholest ...19751220687
metabolism of beta-[3-h]ecdysone during the larval-pupal stage of the blowfly calliphora erythrocephala. 19751126545
linear and non-linear performance of transducer and pupil in calliphora retinula cells.1. intracellular recordings have been made from the blowfly (calliphora erythrocephala) retinula cell; apart from the transducer mechanism, these cells also feature a pupil mechanism. 2. at several mean intensity levels, within the apparently linear range of response, frequency characteristics of amplitude and phase and responses to 'delta'-flashes and 'delta'-flash pairs have been obtained. 3. fourier methods have shown these responses to be mutually compatible, confirming linearity in these ci ...19751142250
independent replication of the ribosomal rna genes in the polytrophic-meroistic ovaries of calliphora erythrocephala, drosophila hydei, and sarcophaga barbata.by filter saturation hybridizations the ribosomal (r)dna contents of the ovaries calliphora erythrocephala, drosophila hydei, and sarcophaga barbata have been measured in comparison to the rdna percentages of their diploid brains. the measurements of the ovarian rdna have been carried out on ovaries where the nurse cells in the distal egg chamber of the ovarioles had reached their highest ploidy level. the diploid rdna content of each of the respective species was chosen as a 100% standard and t ...19751201686
[host range of drosophila melanogaster c virus among diptera and lepidoptera (author's transl)].the host range of the c picornavirus of drosophila melanogaster was studied, using numerous strains of drosophila together with four other genera of diptera and two species of lepidoptera. c virus was injected into the different hosts and serially passaged in them. the extracts from each passage were biologically assayed on virus free d. melanogaster. four different situations were found. 1) a high level of multiplication leading, in 45 strains of drosophilidae, to the early death of the hosts. ...1976823856
juvenile hormone and dna synthesis in imaginal disks of calliphora erythrocephala: results of a new incubation technique. 19761249433
a soluble calcium-binding protein (scbp) present in drosophila melanogaster and calliphora erythrocephala muscle cells.1. soluble calcium binding proteins (scbp) were isolated from homogenates of whole flies, from the thorax and from muscles of drosophila melanogaster and calliphora erythrocephala. 2. crude preparations were obtained by extraction at low ionic strength, acid and heat treatment. the drosophila protein was purified by gel filtration, hydrophobic interaction and ion exchange chromatography. in contrast to calmodulin the drosophila scbp did not bind to phenyl-sepharose in a ca(2+)-dependent way. 3. ...19921499286
dual peptidergic innervation of the blowfly hindgut: a light- and electron microscopic study of fmrfamide and proctolin immunoreactive fibers.1. the innervation of the hindgut, rectal valve, rectum and rectal papillae of the adult blowfly, calliphora erythrocephala, was studied by means of light and electron microscopic immunocytochemistry, using antibodies against the neuropeptides proctolin and fmrfamide. 2. branches from the abdominal nerves reaching the posterior portion of the gut were found to contain mostly neurosecretory type axons and to innervate the muscle coat of all hindgut structures studied. 3. some of the axons found i ...19911685428
cluster organization and response characteristics of the giant fiber pathway of the blowfly calliphora erythrocephala.intersegmental descending neurons (dns) link the insect brain to the thoracic ganglia. iontophoresis of cobalt or fluorescent dyes reveals dns as uniquely identifiable elements, the dendrites of which are situated within a characteristic region of the lateral deutocerebrum. here we demonstrate that dns occur as discrete groups of elements termed dn clusters (dncs). a dnc is a characteristic combination of neurons that arises from a multiglomerular complex in which the main components of each glo ...19902324334
dioptrics of the facet lenses of male blowflies calliphora and chrysomyia.1. the dioptrics of the facet lenses of two blowfly species, calliphora erythrocephala and chrysomyia megacephala, was investigated. measurements were performed on facet lenses ranging in diameter from 20 to 80 microns. 2. the radius of curvature of the front surface of the facet lenses, measured by microreflectometry, increases approximately linearly with the facet lens diameter. 3. the optical path difference of the facet lens and water, measured by interference microscopy, depends on the dist ...19902324995
the fine structure of peritrophic membranes of the blowfly, calliphora erythrocephala, grown in vitro under different conditions. 19761249440
aminergic neurons in the brain of blowflies and drosophila: dopamine- and tyrosine hydroxylase-immunoreactive neurons and their relationship with putative histaminergic neurons.the distribution and morphology of neurons reacting with antisera against dopamine (da), tyrosine hydroxylase (th) and histamine (ha) were analyzed in the blowflies calliphora erythrocephala and phormia terraenovae. th-immunoreactive (thir) and ha-immunoreactive (hair) neurons were also mapped in the fruitfly drosophila melanogaster. the antisera against da and th specifically labeled the same neurons in the blowflies. about 300 neurons displayed da immunoreactivity (dair) and thir in the brain ...19921346506
lanthanum mimicks the trp photoreceptor mutant of drosophila in the blowfly calliphora.the effect of lanthanum on the light response of blowfly (calliphora erythrocephala) photoreceptors was studied. the electrophysiological behaviour of the photoreceptors in the presence of la can be summarized as follows: 1. upon long stimulation the photoreceptors responded with a 'transient receptor potential', i.e. the cells depolarized at the onset of the stimulus and then repolarized to (or below) the resting potential. this effect was dependent on stimulus intensity and occurred only at hi ...19892514264
vasopressin- and proctolin-like immunoreactive efferent neurons in blowfly abdominal ganglia: development and ultrastructure.in the neural sheath of the fused thoracicoabdominal ganglia of the blowfly calliphora erythrocephala, extensive neurohaemal areas can be seen in the electron microscope. a separate set of neurohaemal areas located in the sheath of the lateral abdominal nerve roots contain neural terminals of at least three morphological types. to determine which bioactive substances are stored and possibly released from the neurons supplying these neurohaemal areas, we applied a large number of antisera raised ...19892568372
neural circuits mediating visual flight control in flies. i. quantitative comparison of neural and behavioral response characteristics.the motion-sensitive horizontal cells in the lobula plate of the fly are assumed to play a key role in the sensory control of yaw torque generated by the flying animal during course-stabilization maneuvers and the fixation of objects. this inference results from comparisons of electrophysiological data obtained from blowflies (calliphora erythrocephala) and behavioral data obtained mainly from houseflies (musca domestica) and fruitflies (drosophila melanogaster). apart from few exceptions, the c ...19892585057
the specificity of yolk protein uptake in cyclorrhaphan diptera is conserved through evolution.yolk proteins are transported from the hemolymph into the oocytes of insects during vitellogenesis by receptor-mediated endocytosis. since other hemolymph proteins, both native and foreign, are not accumulated in the oocyte, the process of uptake is selective for yolk proteins. peptide domains within the yolk proteins must therefore be involved in receptor recognition. with the long-term aim of identifying these domains and to open the possibility of understanding the molecular basis of receptor ...19921487828
on the effective optical density of the pupil mechanism in fly photoreceptors.a simple electrophysiological method is described for determining the effective optical density of the intracellular pupil mechanism of insect photoreceptor cells. the method depends on the fact that the photoreceptors can not only be illuminated in the normal, orthodromic way, but also antidromically, i.e. from the back. two assumptions are made: (i) the receptor potential generating process is invariant for the direction of the incident light; (ii) the pupil mechanism is locally driven and onl ...19902402890
[comparative study of hemocyte capsule formation in locusta migratoria, melolontha melolontha and calliphora erythrocephala]. 1977900777
the larval integument of calliphora erythrocephala meigen (diptera : calliphoridae) during metamorphosis. changes in macromolecular content and in incorporation rate of labelled precursors.the amount of proteins, rna and dna in the larval integument of calliphora erythrocephala, decreased markedly when metamorphosis began. the total incorporation per epidermis of labelled precursors also decreased at this time, but far less in rna than in dna and proteins. thus, incorporation, when expressed in terms of specific activity, decreased in proteins, remained stable in dna and increased in rna. the significance of these results is discussed.19862427358
sequence of the calliphora erythrocephala 3b55 satellite: a sequence which is proportionately replicated and transcribed in germ-line polyploid nuclei. 19892587276
experimental reconstruction of calliphora erythrocephala and lucilia sericata life-cycles in the growth cabinet. 19882979436
a stochastic mechanism controls the relative replication of equally competent ribosomal rna gene sets in individual dipteran polyploid nuclei.the endoreplication of the two nucleolar organizers (nos) of the diploid genome has been examined in individual polyploid nuclei of the dipteran calliphora erythrocephala. crosses between two strains with diagnostic nontranscribed spacer polymorphisms in their rrna genes were used to provide progeny with distinguishable nos, and single nuclei of two highly polyploid cell types--salivary gland and nurse cells--were examined from individual f1 animals. initially the representation of the two nos i ...19852991906
both nucleolar organizers are replicated in dipteran polyploid tissues: a study at the level of individual nuclei.working with the dipteran calliphora erythrocephala, we have tested the hypothesis that only one nucleolar organizer region (no) is replicated during polyploidization. no replication was examined in two very different highly polyploid nuclear types: salivary gland nuclei and nurse cell nuclei. two strains of the organism containing no regions with highly diagnostic nontranscribed spacer (nts) polymorphisms were prepared and reciprocal single pair-matings between members of the strains were perfo ...19852996977
enigma of early receptor potential in fly eyes.the early receptor potential (erp) of fly photoreceptors r1-6 has been recorded in the blowfly calliphora erythrocephala, and quantitatively analyzed. photoconversion of the native visual pigment (p) into the meta-state (m) does not induce a measurable erp. photoconversion of m into p induces a biphasic erp, presumably due to the slow thermal decay of intermediate n, recently discovered by roebroek, gagné and stavenga (1989). the experimentally measured erp's are interpreted with an rc-model whi ...19892631387
isolation and characterization of abl gene sequences in calliphora erythrocephala.screening of genomic dna libraries with hybridization probes derived from a drosophila melanogaster c-abl proto-oncogene homologue resulted in the isolation of a set of related sequences from the dipteran calliphora erythrocephala. although the region encompassing the c-abl protein kinase domain encodes a polypeptide extremely similar to the drosophila gene, considerable inter- and intraspecific divergence is found adjacent to this region. restriction-site heterogeneity and cross-hybridization s ...19873125084
gata tandem repeats detect minisatellite regions in blowfly dna (diptera: calliphoridae).a dna probe containing gata tandem repeats detected numerous dispersed minisatellite regions in the genomes of the blowflies chrysomya rufifacies and calliphora erythrocephala. these regions seemed to be actively transcribed into poly(a)+ rna in a tissue-specific manner. when genomic dna of blastoderm embryos was compared with adult genomic dna some loci hybridizing to gata displayed a marked stage-specific variation in length. in calliphora, a small sex-linked dimorphism of gata mini-satellite- ...19882894926
histamine-like immunoreactivity in photoreceptors of the compound eyes and ocelli of the flies calliphora erythrocephala and musca domestica.antibodies to histamine were used for immunocytochemical studies of the visual system in the flies calliphora erythrocephala and musca domestica. specific immunolabeling of photoreceptors was found both in the compound eyes and ocelli of both species. in the compound eyes histamine-like immunoreactivity (ha-ir) was found in all the short visual fibers (photoreceptors r1-6) and one type of long visual fiber (photoreceptor r8). in addition, the ocellar photoreceptors also show ha-ir. in view of ea ...19882902924
light- and electron-microscopic immunocytochemistry of peptidergic neurons innervating thoracico-abdominal neurohaemal areas in the blowfly.ventral thoracic neurosecretory cells (vtncs) of the blowflies, calliphora erythrocephala and c. vomitoria, innervating thoracic neuropil and the dorsal neural sheath of the thoracico-abdominal ganglion have been shown to be immunoreactive to a variety of mammalian peptide antisera. in the neural sheath the vtnc terminals form an extensive neurohaemal network that is especially dense over the abdominal ganglia. the same areas are invaded by separate, but overlapping serotonin-immunoreactive (5-h ...19883180185
3b55: a repetitious sequence family which is transcribed and proportionately replicated in germ-line polyploid nuclei of calliphora erythrocephala.the chromosomes of dipteran polyploid nurse cell nuclei are functionally analogous to the oocyte lampbrush chromosomes of the amphibia. in investigating the transcriptional and replicative activity of these nuclei in calliphora erythrocephala we have identified a cloned highly repetitious dna fragment which shows enhanced transcriptional activity in these cells and different replicative behavior in these germ-line polyploid nuclei as opposed to somatic polytene nuclei. the clone, 3b55, contains ...19863011566
metamorphosis of identified neurons innervating thoracic neurohemal organs in the blowfly: transformation of cholecystokininlike immunoreactive neurons.with antisera to gastrin/cholecystokinin, we studied the postembryonic development of neurons in the thoracic ganglia of the blowfly calliphora erythrocephala. there are some changes in the population of thoracico-abdominal neurons displaying gastrin/cck-like immunoreactivity (cckli): some cckli neurons cannot be found after pupariation; other neurons become immunoreactive during metamorphosis. six large thoracic cckli neurons could, however, be followed through metamorphosis. these cckli neuron ...19883343405
quantitative analysis of resting membrane electrogenesis in insect (diptera) skeletal muscle. i. intracellular k+, na+ and cl- activities, measured using liquid ion-exchanger and neutral ion-carrier microelectrodes.electrophysiological properties of skeletal body-wall muscles of prepupal calliphora erythrocephala were investigated using double-barrelled intracellular ion-sensitive microelectrodes. the most realistic estimate of the intracellular k+ activity, obtained using k+-sensitive microelectrodes based on a neutral carrier, was 115 mmoll-1. the k+ equilibrium potential was consistently more negative than the prevailing resting potential, the average difference being -15 mv. the intracellular na+ activ ...19883404076
quantitative analysis of resting membrane electrogenesis in insect (diptera) skeletal muscle. ii. testing of a model involving contributions from potassium and sodium ions, and the anomalous effect of reducing extracellular sodium.a model of resting membrane electrogenesis in skeletal muscles of prepupal calliphora erythrocephala was formulated. from experiments in which reversible effects of changing extracellular k+ and na+ activities on the membrane potential (em) were measured, three different values of alpha (the ratio of the partial permeabilities of the membrane to na+ and k+) were derived, each from a different range of extracellular na+ and k+ activities. two independent tests were carried out to determine the mo ...19883404077
light and electron microscopic immunocytochemistry of neurons in the blowfly optic lobe reacting with antisera to rfamide and fmrfamide.different antisera to the molluscan cardioexcitatory peptide fmrfamide, and its fragment, rfamide (arg-phe-nh2), label a distinct population of neurons in the optic lobe of the blowfly, calliphora erythrocephala. seven morphological types of rfamide/fmrfamide-like immunoreactive neurons could be distinguished in the optic lobes based on the locations of their cell bodies, their axonal projections and the distribution of their processes. of these, two types could be resolved in their entire exten ...19883200445
genes with specific functions in the ovarian follicles of calliphora erythrocephala (diptera).working with the large dipteran calliphora erythrocephala, we have performed differential screening to identify genes actively expressed in the previtellogenic and early vitellogenic stages of oogenesis but silent during the early stages of embryogenesis. clones containing sequences homologous to four such genes have been characterized. two clones are homologous to the yolk protein 1 gene of drosophila melanogaster. these two clones are expressed not only in the columnar follicle cells surroundi ...19883417047
non-linearities in response properties of insect visual cells: an analysis in time and frequency domain.intracellularly recorded voltage responses of the visual cells of the blowfly (calliphora erythrocephala) were analysed in the time and frequency domains. the photoreceptors were stimulated with pulse (impulse), sine, sine-sweep and pseudorandomly (white noise) modulated green light. the blowfly photoreceptor responses, as analysed from the linear transfer functions, seem to arise from a system similar to that of cascaded low-pass filters, with a corner frequency at about 63 hz (sd +/- 12 hz). t ...19883223300
proctolin-like immunoreactive neurons in the blowfly central nervous system.the pentapeptide proctolin (h-arg-tyr-leu-pro-thr-oh) is a well-studied bioactive substance in insects. with an antiserum against proctolin we have mapped proctolinlike-immunoreactive (pli) neurons in the nervous system of the blowfly calliphora erythrocephala. in the brain, including the suboesophageal ganglia, 80-90 neurons were found to be pli. a further 200-250 pli neurons innervate the lobula of the optic lobe. the thoracic ganglia contain 100-130, and the abdominal ca. 60 pli neurons. in t ...19873693615
dna sequence analysis of an abl-related gene in the blow fly, calliphora erythrocephala.the ceabl locus is a member of a small family of abl-related sequences in c. erythrocephala. the catalytic, sh2 and sh3 domains of the ceabl-encoded protein show greater than 75% sequence identity to vertebrate abl protein tyrosine kinases and greater than 95% identity to the d. melanogaster abl polypeptide. ceabl diverges from the vertebrate proteins, however, at the extreme amino terminus, near the beginning of the vertebrate abl exon involved in differential splicing. the carboxyl region show ...19901367423
late asthmatic reactions due to larvae of calliphora erythrocephala used as fishing bait.clinical characteristics of allergic asthma due to inhalation of insect antigens have been investigated very little. we describe two patients presenting with dyspnoea and wheezing several hours after exposure to larvae of calliphora erythrocephala used as fishing bait. both patients had a positive intradermal skin test and a high level of circulating ige to the specific allergen. bronchial challenge test with the whole larvae resulted in an isolated late response in one case, and in a dual respo ...19873604840
serotonin and gastrin/cholecystokinin-like immunoreactive neurons in the larval retrocerebral complex of the blowfly calliphora erythrocephala.the presence and distribution of neurons immunoreactive against antibodies to serotonin (5-ht) and gastrin/cholecystokinin (gastrin/cck) has been studied in the larval retrocerebral complex of the blowfly calliphora erythrocephala, a composite structure which consists of the corpus cardiacum, the corpus allatum, the thoracic gland and a portion of the cephalic aorta. immunoreactive material was found in all these elements except in the corpus allatum. six to eight cell bodies in the corpus cardi ...19883409294
photoreceptor redox state monitored in vivo by transmission and fluorescence microspectrophotometry in blowfly compound eyes.the transmission and fluorescence of the compound eye of living, intact blowflies calliphora erythrocephala, mutant chalky, were studied microspectrophotometrically. transmission spectra were recorded under four conditions. the fly was either in the normal air environment or in a nitrogen atmosphere, and in both cases the investigated eye was adapted to red and blue light, respectively. the absorbance difference spectra obtained from the two chromatic adapted conditions showed the clear characte ...19863716217
electron microscopic localization of chitin using colloidal gold labelled with wheat germ agglutinin.the lectin wheat germ agglutinin (wga) has a binding site which is able to bind a sequence of three n-acetyl-glucosamine residues. therefore, it has a very strong affinity for the polymers of this sugar, especially chitin. colloidal gold can be labelled with wga and used as a specific electron-dense marker for the electron-microscopic localization of chitin. the specificity of the wga-gold binding can be checked by competitive inhibition with 5-10 mm triacetyl chitotriose. the reliability of thi ...19863754855
dopamine-beta-hydroxylase-like immunoreactive neurons in two insect species, calliphora erythrocephala and periplaneta americana.the localization of dopamine-beta-hydroxylase in the cephalic central nervous system of the blowfly (calliphora erythrocephala) and the cockroach (periplaneta americana) was investigated. immunoreactive neurons were demonstrated in both species. the results were compared with the known distribution of catecholamines in the brain of both species. in certain cell groups and neuropilar regions of both species d beta h-immunoreactivity coincides with the presence of catecholamines. additionally d be ...19853930439
the stiles-crawford effect in the eye of the blowfly, calliphora erythrocephala.stiles-crawford-like effects (that is, directional sensitivity of the retina) were investigated in the fly's eye. intracellular recordings from the visual sense cells were made, and the radiation patterns emerging from the photoreceptors with antidromic light were photographed, and evaluated with a microdensitometer. the measurements from both methods agree well, and can be satisfactorily described by a theoretical model based on waveguide theory. clear radiation patterns from the first and seco ...19854072011
purification and properties of a phenol oxidase from the blowfly calliphora erythrocephala. 19734123357
investigations on the transport function and structure of peritrophic membranes. 3. periodic incorporation of glucose, methionine and cysteine into the peritrophic membranes of the blowfly calliphora erythrocephala mg. in vivo and in vitro. 19734123259
biosynthesis of unsaturated and saturated fatty acids in aseptically reared larvae of calliphora erythrocephala (meig.). 19654158417
biosynthesis of the fatty acids in aseptically reared larvae of the blowfly calliphora erythrocephala (meig.). 19664165980
receptor turnover and the action of 5-hydroxytryptamine on the salivary glands of the blowfly calliphora erythrocephala, the housefly musca domestica and frog skin epithelium.1. continual stimulation of frog skin epithelium and the salivary glands of the insects calliphora and musca with 5-hydroxytryptamine (5-ht) leads to desensitisation, i.e. the tissue fails to respond to the application of further 5-ht. 2. incubation of desensitised frog skin and musca salivary glands with either n-acetyl neuraminic acid or inositol partially restored the 5-ht responses whilst incubation with a combination of n-acetyl neuraminic acid and inositol gave additive effects on the reco ...19872893678
the neurosecretory system of the adult calliphora erythrocephala. iv. a histological study of the corpus cardiacum and its connections with the nervous system. 19694190435
[ultrastructural aspects of the integumentary histoblast areas of the abdomen of calliphora erythrocephala during the first larval stage]. 19744219150
role of the "thrombocytoids" in capsule formation in the dipteran calliphora erythrocephala.of the three hemocyte types present in the blood of calliphora, only one participates in capsule formation around implanted cellophane. this hemocyte, the thrombocytoid, shows in the blood a tendency to dissociate into numerous small cytoplasmic fragments, comparable to the mammalian megakaryocyte. this tendency is dramatically increased during the process of encapsulation. most of the intact thrombocytoids and the numerous fragments participating in capsule formation do not show any particular ...19751182767
[a study of the lysosomial acid hydrolases of pupae of diptera calliphora erythrocephala]. 19664168226
changes in the functional efficiency of flight muscle sarcosomes during heat death of adult calliphora erythrocephala. 19714255709
[histochemical investigations of the eye of the blowfly calliphora erythrocephala meig. ii. nucleosidephosphatases, thiaminepyrophosphatase, nonspecific esterase, and cholinesterase]. 19724262566
dopamine-immunoreactive neurons in the blowfly visual system: light and electron microscopic immunocytochemistry.dopamine-immunoreactive (da-ir) neurons were mapped in detail in the visual system of the blowfly, calliphora erythrocephala. three types of da-ir neurons could be identified in the optic lobes. one type constitutes a population of several thousand columnar small field amacrine neurons in the second neuropil region, the medulla. the other two types are large field projection neurons innervating the next, more central, synaptic region comprising the lobula and the lobula plate, as well as centres ...19883270359
the localization of myofibrillar atpase activity in the flight muscles of the blowfly, calliphora erythrocephala.the distribution of atpase activity in the asynchronous flight muscles of calliphora erythrocephala (diptera) was studied at a fine structural level, using preparations of teased fibers, both unfixed and after brief fixation in hydroxyadipaldehyde, incubated in a medium for the histochemical demonstration of myosin or actomyosin atpase. in relaxed fibrils, activity was found confined to the a bands and was absent from the h zones as well as from the z and i band regions. at high magnification, d ...19654221034
ultrastructure of the midgut epithelial cells in the fly calliphora erythrocephala. 19714329464
changes taking place in the respiratory efficiency of isolated flight muscle sarcosomes, associated with the age of the blowfly, calliphora erythrocephala. 19674229880
biochemical and structural variations in the flight muscle mitochondria of ageing blowflies, calliphora erythrocephala. 19724336113
the ultrastructure and function of pericardial cells and other nephrocytes in an insect: calliphora erythrocephala. 19724348054
the haemocytes of calliphora erythrocephala (meig.) (diptera). 19734355160
[histochemical studies on the eye of the blowfly calliphora erythrocephala meig. i. atp-ase, acid phosphatase, alkaline phosphatase and glucose-6-phosphatase]. 19714332957
[ecdysone titer during insect development. vi. studies on the distribution of ecdysone in various tissues of calliphora erythrocephala and on its biologic half-life]. 19694390021
ultrastructural changes in developing midgut epithelium of calliphora erythrocephala meigen. 19724339566
the abdominal epidermis of calliphora erythrocephala (diptera). i. polyteny and growth in the larval cells. 19744417501
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