| targeting conserved genes in fusarium species. | fumonisins are important mycotoxins contaminating foods and feeds which are mainly produced by f. verticillioides and f. proliferatum. additionally, both are pathogens of maize and other cereals. we describe two highly sensitive, rapid, and species-specific pcr protocols which enable detection and discrimination of these closely related species in cereal flour or grain samples. the specific primer pairs of these assays were based on the intergenic spacer region of the multicopy rdna unit which h ... | 2017 | 27924535 |
| ungual hyalohyphomycosis caused by fusarium proliferatum in an immunocompetent patient. | the patient was a 73-year-old healthy female farmer who had been treated with terbinafine for 25.5 months by a primary physician. she exhibited a discoloration and thickening of the right big toenail. she had no concomitant paronychia. direct microscopy revealed chlamydoconidia and hyphae, and periodic acid-schiff stained nail specimen showed septate hyphae. on the basis of these morphological features and gene analysis, the final diagnosis was ungual hyalohyphomycosis caused by fusarium prolife ... | 2017 | 27607919 |
| a novel population of fusarium fujikuroi isolated from southeastern u.s. winegrapes reveals the need to re-evaluate the species' fumonisin production. | mycotoxins pose a challenge to a safe food supply worldwide, and their threat is expected to worsen with our changing climate. the need for diligence is exemplified by the discovery of fumonisin b2 in wine, which joins ochratoxin a as a mycotoxin of concern in the grape-wine chain. to elucidate the mycotoxin risk in southeastern american wine, grape samples were collected from vineyards during harvest in 2013 and potentially mycotoxigenic fungi (fusarium and aspergillus) were isolated from the s ... | 2016 | 27589800 |
| histopathological assessment of the infection of maize leaves by fusarium graminearum, f. proliferatum, and f. verticillioides. | young maize plants were inoculated on unfolded mature leaves and on folded immature leaves with fusarium graminearum, fusarium proliferatum, and fusarium verticillioides suspensions. infection and symptom development of disease on these asymptomatic mature leaves and immature leaves were then documented. subcuticular infection was found by the three fusarium species on both symptomatic and symptomless leaves. the three fusarium species penetrated the stomata of immature leaves by the formation o ... | 2016 | 27567716 |
| species-specific optical genosensors for the detection of mycotoxigenic fusarium fungi in food samples. | plant-pathogenic fusarium species, fusarium verticillioides and fusarium proliferatum, are the major producers of fumonisins which are one of the most common mycotoxins found in maize. herein, we report the development of specific and sensitive genosensors for detecting these two closely related fusarium species in food samples. the sensors are based on species-specific capture and detection probes, which bind to the intergenic spacer region of rdna (igs). oligonucleotide functionalized magnetic ... | 2016 | 27543032 |
| cloning and functional characterization of nitrilase from fusarium proliferatum auf-2 for detoxification of nitriles. | a fungal nitrilase gene from fusarium proliferatum auf-2 was cloned through reverse transcription-pcr. the open reading frame consisted of 903 bp and potentially encoded a protein of 301 amino acid residues with a theoretical molecular mass of 33.0 kda. the encoding gene was expressed in escherichia coli strain bl21 and the recombinant protein with his6-tag was purified to electrophoretic homogeneity. the purified enzyme exhibited optimal activity in the range of 35-40 °c and ph 8.0. edta, mg(2+ ... | 2015 | 25595333 |
| inhibition of the phytopathogenic fungus fusarium proliferatum by volatile compounds produced by pseudomonas. | the fusarium head blight of grain cereals is a significant disease worldwide. in argentina, high levels of contamination with fusarium proliferatum have been found in crops. many strains of the pseudomonas genus antagonize the growth of fungi by different mechanisms, such as the production of antibiotics, siderophores, volatiles, and extracellular enzymes. in this work, we have designed a new system for studying the growth inhibition of f. proliferatum-namely by volatile compounds produced by ps ... | 2014 | 25085617 |
| vacuolar serine protease is a major allergen of fusarium proliferatum and an ige-cross reactive pan-fungal allergen. | fusarium species are among prevalent airborne fungi and causative agents of human respiratory atopic disorders. we previously identified a 36.5-kda f. proliferatum component recognized by ige antibodies in 9 (53%) of the 17 f. proliferatum-sensitized atopic serum samples. the purpose of this study is to characterize the 36.5-kda allergen of f. proliferatum. | 2016 | 27334782 |
| antifungal attributes of lactobacillus plantarum mys6 against fumonisin producing fusarium proliferatum associated with poultry feeds. | fumonisins, being common in occurrence in maize-based feeds, pose a great threat to animal and human health. the present study is aimed at determining the antifungal activity of lactobacillus plantarum mys6 against a fumonisin producing fungus, fusarium proliferatum mys9. the isolate was subjected to standard tests for determining its probiotic attributes and antifungal properties. l. plantarum mys6 thrived well at ph 3.0 and 6.0, and exhibited strong resistance up to 3% bile. the isolate showed ... | 2016 | 27285317 |
| fusarium proliferatum strains change fumonisin biosynthesis and accumulation when exposed to host plant extracts. | fumonisin concentrations in mycelia and media were studied in liquid fusarium proliferatum cultures supplemented with host plant extracts. furthermore, the kinetics of fumonisin accumulation in media and mycelia collected before and after extract addition was analysed as well as the changes in the expression of the fum1 gene. fumonisin content in culture media increased in almost all f. proliferatum strains shortly after plant extracts were added. the asparagus extract induced the highest fb lev ... | 2016 | 27268248 |
| new cyclohexadepsipeptides from an entomogenous fungus fusarium proliferatum and their cytotoxicity and autophagy-inducing activity. | five new cyclohexadepsipeptides termed as enniatins r - v (1 - 5) and seven known cyclohexadepsipeptides (6 - 12) were isolated from the solid culture of fusarium proliferatum, a fungus isolated from the cadaver of an unidentified insect collected in tibet. their structures were elucidated by nmr and ms spectroscopic analysis. the x-ray single-crystal structure of 6 was reported for the first time. enniatins r and s represented the first enniatins incorporating with an unusual 2,3-dihydroxy-isov ... | 2016 | 27233912 |
| inhibitory mechanism of butylated hydroxyanisole against infection of fusarium proliferatum based on comparative proteomic analysis. | fusarium proliferatum as a filamentous fungal pathogen can produce mycotoxins that can contaminate postharvest fruits and thus impact risks on human health. the extracellular proteomes of f. proliferatum grown in the absence and presence of butylated hydroxyanisole (bha) were analyzed comparatively. a total of 66 significantly different expressed secreted proteins were identified by lc-esi-ms/ms analysis. the bha treatment suppressed the accumulation of some pathogenic factors such as aspartic p ... | 2016 | 27169933 |
| development of simple sequence repeat (ssr) markers for discrimination among isolates of fusarium proliferatum. | the plant pathogen fusarium proliferatum has a wide host range and occurs worldwide. many isolates of the fungus produce mycotoxins in plant tissues, which, if ingested, can cause harm to animals and humans. in 2008, an outbreak of salmon blotch of onions, caused by f. proliferatum, was detected in southern israel. the source and distribution of the fungus in israel were unknown. inter-simple sequence repeats (issr) were used to identify repetitive motifs present in seven isolates of f. prolifer ... | 2016 | 27021663 |
| evaluation of ability of ferulic acid to control growth and fumonisin production of fusarium verticillioides and fusarium proliferatum on maize based media. | the aim of this study was to evaluate the efficacy of ferulic acid (1, 10, 20 and 25 mm) at different water activity (aw) values (0.99, 0.98, 0.96 and 0.93) at 25 °c on growth and fumonisin production by fusarium verticillioides and fusarium proliferatum on maize based media. for both fusarium species, the lag phase significantly decreased (p ≤ 0.001), and the growth rates increased (p ≤ 0.001) at the lowest ferulic acid concentration used (1mm), regardless of the aw. however, high doses of feru ... | 2013 | 24140805 |
| development of a sensitive, competitive, indirect elisa for the detection of fumonisin b1 in corn originating from anhui province, china. | fumonisin b1 (fb1) is a secondary metabolite produced by fusarium verticillioides or fusarium proliferatum, which present in food and feed. it causes hazardous effects on human and animal health. a monoclonal antibody (mab) against fb1 was produced and a simple, reliable and sensitive, competitive, indirect enzyme-linked immunosorbent assay (ci-elisa) for detection of fb1 was developed and the experiment conditions were optimized. the coating concentration of fb1-ovalbumin (fb1-ova) was 500 ng m ... | 2016 | 26621078 |
| effects of fusaric acid treatment on the protocorm-like bodies of dendrobium sonia-28. | dendrobium sonia-28 is a popular orchid hybrid due to its flowering recurrence and dense inflorescences. unfortunately, it is being decimated by fungal diseases, especially those caused by fusarium proliferatum. in this study, selection of f. proliferatum-tolerant protocorm-like bodies (plbs) was carried out by assessing the effects of differing concentrations of fusaric acid (fa). plbs were cultured on murashige and skoog (ms) medium supplemented with 0.05 to 0.2 millimolar (mm) concentrations ... | 2016 | 26471909 |
| a new isolate from fusarium proliferatum (auf-2) for efficient nitrilase production. | a fungal isolate from fusarium proliferatum strain auf-2 has been found to have a high nitrilase activity (≥ 1,000 u/l culture). the present work describes optimization of growth conditions and production medium to achieve maximum nitrilase production. the most effective carbon and nitrogen sources for nitrilase production were glucose and sodium nitrate, respectively. ε-caprolactam was found to be the best inducer for maximum nitrilase production with 80 g/l wet cell biomass and 26 u/g nitrilas ... | 2013 | 23934068 |
| urinary tract infection (uti) caused by fusarium proliferatum in an agranulocytosis patient and a review of published reports. | infections caused by fusarium species are increasing in frequency among immunocompromised hosts, but urinary tract infection (uti) due to fusarium proliferatum has not been reported in the literature so far. we describe a case of uti caused by f. proliferatum in a 47-year-old man who was diagnosed with rectal cancer and metastasis. he underwent radical resection of rectal carcinoma and local resection of hepatic metastases. after the first adjuvant chemotherapy, the patient presented the obvious ... | 2016 | 26346378 |
| dermatitis in the fringe-toed lizard, acanthodactylus nilsoni rastegar-pouyani, 1998 (sauria: lacertidae) associated with fusarium proliferatum. | from july to october 2013, nine out of 40 acanthodactylus nilsoni collected from western iran, showed clinical signs of dermatitis in the dorsal and ventral surface of neck and fingers. therefore, the aim of this survey was to identify the fungal flora colonizing the skin of a. nilsoni using morphological and molecular studies. nine isolates of fusarium were obtained from infected lizard samples and identified as fusarium proliferatum through study of morphological characters. in the present stu ... | 2015 | 26292787 |
| bioactive compounds from the endophytic fungus fusarium proliferatum. | the crude extract of an endophytic fungus isolated from syzygium cordatum and identified as fusarium proliferatum showed 100% cytotoxicity against the brine shrimp artemia salina at 100 μg/ml. seven coloured, biologically active metabolites - including ergosta-5,7,22-trien-3β-ol, nectriafurone-8-methyl ether, 9-o-methyl fusarubin, bostrycoidin, bostrycoidin-9-methyl ether and 8-hydroxy-5,6-dimethoxy-2-methyl-3-(2-oxo-propyl)-1,4-naphthoquinone- were isolated from the extract. | 2016 | 26158312 |
| fungal endophytes of vanilla planifolia across réunion island: isolation, distribution and biotransformation. | the objective of the work was to characterize fungal endophytes from aerial parts of vanilla planifolia. also, to establish their biotransformation abilities of flavor-related metabolites. this was done in order to find a potential role of endophytes on vanilla flavors. | 2015 | 26070309 |
| a case of fungus ball-type pansinusitis due to fusarium proliferatum. | incidence of fungal sinusitis due to the genus fusarium has increased during the last two decades. we report a case of fungus ball sinusitis with multiple sinuses involvement in an iranian 21-year-old woman. the patient was diagnosed as having a fungus ball-type sinusitis in computed tomography scan. the sinus biopsy revealed fungal structures on histopathological and direct microscopic examinations and a fusarium species arose in culture. partial sequencing of the translation elongation factor ... | 2015 | 26025662 |
| fumonisin b1 induces autophagic cell death via activation of ern1-mapk8/9/10 pathway in monkey kidney marc-145 cells. | mycotoxins are secondary fungal metabolites that are capable of inducing a variety of toxic effects in animals and humans resulting from the consumption of the contaminated food. understanding the mechanisms of the toxicities behind these mycotoxins is required to develop mechanism-based approach to counteract their toxic potential. fumonisin b1 (fb1) is the most prevalent member of fumonisins that are a group of mycotoxins produced primarily by fusarium verticillioides and fusarium proliferatum ... | 2016 | 25925693 |
| fungal rhino sinusitisin in tehran, iran. | fungal rhino sinusitis (frs) is an important infection of para nasal sinuses, which encompasses two main categories; invasive and noninvasive forms according to histopathological findings. aspergillus spp are the most common species isolated from noninvasive form, while mucorales are more frequently isolates from acute infections. | 2015 | 25905081 |
| [isolation of endophytic fungi from macleaya cordata and screening of sanguinarine-producing strains]. | endophytic fungi were isolated from macleaya cordata growing in dabie mountain by agar-block method, and then the endophytic fungi were grouped into different types based on their morphological characteristics, and thin layer chromatography (tlc) and high performance liquid chromatography (hplc) were employed to determine whether the metabolic substances contained sanguinarine or not, and then preliminarily identified by morphological method. the results showed that the leaves hosted the largest ... | 2014 | 25850254 |
| concatemerization increases the inhibitory activity of short, cell-penetrating, cationic and tryptophan-rich antifungal peptides. | there are short cationic and tryptophan-rich antifungal peptides such as the hexapeptide paf26 (rkkwfw) that have selective toxicity and cell penetration properties against fungal cells. this study demonstrates that concatemeric peptides with tandem repeats of the heptapeptide paf54 (which is an elongated paf26 sequence) show increased fungistatic and bacteriostatic activities while maintaining the absence of hemolytic activity of the monomer. the increase in antimicrobial activity of the double ... | 2015 | 25846331 |
| carbon and nitrogen limitation increase chitosan antifungal activity in neurospora crassa and fungal human pathogens. | chitosan permeabilizes plasma membrane and kills sensitive filamentous fungi and yeast. membrane fluidity and cell energy determine chitosan sensitivity in fungi. a five-fold reduction of both glucose (main carbon (c) source) and nitrogen (n) increased 2-fold neurospora crassa sensitivity to chitosan. we linked this increase with production of intracellular reactive oxygen species (ros) and plasma membrane permeabilization. releasing n. crassa from nutrient limitation reduced chitosan antifungal ... | 2015 | 25749367 |
| genetic variability and fumonisin production by fusarium proliferatum isolated from durum wheat grains in argentina. | fusarium proliferatum is a member of the fusarium fujikuroi species complex (ffsc) involved in the maize ear rot together with fusarium verticillioides, which is a very closely related species. recently, different studies have detected natural fumonisin contamination in wheat kernels and most of them have shown that the main species isolated was f. proliferatum. fusarium strains obtained from freshly harvested durum wheat samples (2008 to 2011 harvest seasons) from argentina were characterized t ... | 2015 | 25732000 |
| influence of butylated hydroxyanisole on the growth, hyphal morphology, and the biosynthesis of fumonisins in fusarium proliferatum. | fusarium proliferatum as a common fungus pathogen in foods can produce toxic fumonisins, which can cause animal diseases and increase risks of human cancers. on contrary, butylated hydroxyanisole (bha) as a synthetic antioxidant offers a clue for preventing growth of fungal species and inhibiting production of mycotoxins. unfortunately, information of the inhibitory mechanism of bha on fusarium species is still limited. in this study, influence of bha treatment on growth and inhibition of fumoni ... | 2016 | 27468276 |
| host extract modulates metabolism and fumonisin biosynthesis by the plant-pathogenic fungus fusarium proliferatum. | fusarium proliferatum is a common pathogen able to infect a broad range of agriculturally important crops. recently, some evidence for genetic variance among the species genotypes in relation to their plant origin has been reported. mycotoxin contamination of plant tissues is the most important threat caused by f. proliferatum and fumonisins b (fbs) are the principal mycotoxins synthesized. the toxigenic potential of the pathogen genotypes is variable and also the reaction of different host plan ... | 2015 | 25462926 |
| the novel strain fusarium proliferatum le1 (rcam02409) produces α-l-fucosidase and arylsulfatase during the growth on fucoidan. | enzymes capable of modifying the sulfated polymeric molecule of fucoidan are mainly produced by different groups of marine organisms: invertebrates, bacteria, and also some fungi. we have discovered and identified a new strain of filamentous fungus fusarium proliferatum le1 (deposition number in russian collection of agricultural microorganisms is rcam02409), which is a potential producer of fucoidan-degrading enzymes. the strain le1 (rcam02409) was identified on the basis of morphological chara ... | 2015 | 25346501 |
| fungaemia caused by fusarium proliferatum in a patient without definite immunodeficiency. | recent literature has shown the growing importance of opportunistic fungal infections due to fusarium spp. however, disseminated fusariosis remains rare in patients without neutropenia. we report a case of fungaemia in a 78-year-old french woman without definite immunodeficiency. fusarium proliferatum grew from both central and peripheral blood cultures. fever was the only clinical sign of the infection. an appropriate antifungal therapy with voriconazole led to the recovery of the patient. an e ... | 2015 | 25253233 |
| quantitative determination of fusarium proliferatum concentration in intact garlic cloves using near-infrared spectroscopy. | fusarium proliferatum is considered to be a pathogen of many economically important plants, including garlic. the objective of this research was to apply near-infrared spectroscopy (nirs) to rapidly determine fungal concentration in intact garlic cloves, avoiding the laborious and time-consuming procedures of traditional assays. preventive detection of infection before seeding is of great interest for farmers, because it could avoid serious losses of yield during harvesting and storage. spectra ... | 2016 | 27428978 |
| experimental infection of fusarium proliferatum in oryza sativa plants; fumonisin b1 production and survival rate in grains. | fusarium proliferatum is a plant pathogenic fungus associated with crops such as asparagus and corn, and it possesses the ability to produce a range of mycotoxins, including fumonisins. in asia, rice (oryza sativa) is a staple cereal and is occasionally colonized by this fungus without obvious physiological changes. f. proliferatum is closely related to gibberella fujikuroi (anamorph f. fujikuroi) responsible for bakanae disease in rice; however there are few reports of f. proliferatum as a rice ... | 2012 | 22534354 |
| two-dimensional environmental profiles of growth and fumonisin production by fusarium proliferatum on a wheat-based substrate. | the effect of water activity (aw; 0.995, 0.99, 0.98, 0.96, 0.94, 0.92, and 0.90), temperature (15, 25, and 30°c), incubation time (7, 14, 21 and 28days), and their interactions on mycelial growth and fumonisin production on wheat-based medium by three fusarium proliferatum strains isolated from wheat in argentina was evaluated. maximum growth rates were obtained at the highest aw (0.995) and 30°c, with growth decreasing as the aw of the medium was reduced. maximum amounts of total fumonisins (fb ... | 2014 | 24854387 |
| interactions between a sap beetle, sabal palm, scale insect, filamentous fungi and yeast, with discovery of potential antifungal compounds. | the multi-trophic relationship between insects, yeast, and filamentous fungi is reported on sabal palm (sabal palmetto (walter) lodd. ex schult. & schult. f.). gut content analyses and observations of adult and larval feeding of the sap beetle brachypeplus glaber leconte indicate that niche partitioning of fungal food substrata occurs between adults and larvae. this is the first report of specific mycophagous niche partitioning among beetle life stages based on gut content analyses. fungi isolat ... | 2014 | 24586669 |
| metabolism and resistance of fusarium spp. to the manzamine alkaloids via a putative retro pictet-spengler reaction and utility of the rational design of antimalarial and antifungal agents. | as a part of our continuing investigation of the manzamine alkaloids we studied the in vitro activity of the β-carboline containing manzamine alkaloids against fusarium solani, fusarium oxysporium, and fusarium proliferatum by employing several bioassay techniques including one-dimensional direct bioautography, dilution, and plate susceptibility, and microtiter broth assays. in addition, we also studied the metabolism of the manzamine alkaloids by fusarium spp. in order to facilitate the redesig ... | 2014 | 24553735 |
| granulomatous pododermatitis in the digits caused by fusarium proliferatum in a cat. | to the best of our knowledge, we present here the first report of a case involving granulomatous pododermatitis caused by fusarium proliferatum in a 10-year-old female cat. a cutaneous mass developed on the foot-pad of the right hind leg. nodular granulomatous dermatitis with numerous macrophages and multinucleated giant cells containing cytoplasmic fungal structures were revealed on histological examination. periodic acid-schiff reaction and fungi-fluor staining clearly revealed irregular, sept ... | 2014 | 24200892 |
| an endophytic sanguinarine-producing fungus from macleaya cordata, fusarium proliferatum blh51. | fermentation processes using sanguinarine-producing fungi other than macleaya cordata may be an alternative way to produce sanguinarine (sa), which is a quaternary benzo[c]phenanthridine alkaloid possessing antibacterial, anthelmintic, and anti-inflammatory properties. in this study, a sa-producing endophytic fungus strain blh51 was isolated from the leaves of m. cordata grown in the dabie mountain, china. strain blh51 produced sa when grown in potato dextrose liquid medium. the amount of sa pro ... | 2014 | 24166154 |
| [screening and condition optimization of a strain for efficiently biotransformation of saponins in dioscorea zingiberensis into diosgenin]. | diosgenin is an important raw material in steroid hormone and widely used in pharmaceutical industry. the traditional method for diosgenin production is acidolysis, which causes serious pollution. in order to obtain a cleaner and more efficient approach of diosgenin production, a strain of gibberella intermedia wx12 (the sexual stage of fusarium proliferatum) was screened from the strains deposited in our laboratory. this strain converted saponins in dioscorea zingiberensis c.h. wright (dzw) int ... | 2013 | 24063243 |
| light affects fumonisin production in strains of fusarium fujikuroi, fusarium proliferatum, and fusarium verticillioides isolated from rice. | three fusarium species associated with bakanae disease of rice (fusarium fujikuroi, fusarium proliferatum, and fusarium verticillioides) were investigated for their ability to produce fumonisins (fb1 and fb2) under different light conditions, and for pathogenicity. compared to darkness, the conditions that highly stimulated fumonisin production were yellow and green light in f. verticillioides strains; white and blue light, and light/dark alternation in f. fujikuroi and f. proliferatum strains. ... | 2013 | 24055868 |
| enhancing nitrilase production from fusarium proliferatum using response surface methodology. | the individual and interactive effects of three independent variables i.e. carbon source (glucose), nitrogen source (sodium nitrate) and inducer (ϵ-caprolactam) on nitrilase production from fusarium proliferatum were investigated using design of experiments (doe) methodology. response surface methodology (rsm) was followed to generate the process model and to obtain the optimal conditions for maximum nitrilase production. based on central composite design (ccd) a quadratic model was found to fit ... | 2013 | 23853757 |
| potential effects of environmental conditions on the efficiency of the antifungal tebuconazole controlling fusarium verticillioides and fusarium proliferatum growth rate and fumonisin biosynthesis. | fusarium verticillioides and fusarium proliferatum are important phytopathogens which contaminate cereals in the mediterranean climatic region with fumonisins. in this study we examined the interaction between the fungicide efficacy of tebuconazole and water potential (ψw) (-0.7-7.0mpa)×temperature (20-35°c) on growth and fum1 gene expression by real time rt-pcr (an indicator of fumonisin biosynthesis) in strains of both fusarium species. concentrations of tebuconazole required to reduce growth ... | 2013 | 23800737 |
| structural variation and dynamics of the nuclear ribosomal intergenic spacer region in key members of the gibberella fujikuroi species complex. | the intergenic spacer (igs) region of the ribosomal dna was cloned and sequenced in eight species within the gibberella fujikuroi species complex with anamorphs in the genus fusarium, a group that includes the most relevant toxigenic species. dna sequence analyses revealed two categories of repeated elements: long repeats and short repeats of 125 and 8 bp, respectively. long repeats were present in two copies and were conserved in all the species analyzed, whereas different numbers of short repe ... | 2013 | 23706073 |
| characterization of gibberellin biosynthetic gene cluster from fusarium proliferatum. | gibberellins (gas) are a group of phytohormones that control many developmental processes in higher plants. we report the cloning and expression pattern of gibberellin biosynthesis genes from a new ga-producing fungus, fusarium proliferatum (strain kgl0401). these genes sequences are deposited in the national center for biotechnology information (ncbi) under accession numbers ef119831, ef119832, dq313173, dq313174, dq313175, dq313176, and dq313177. the expression level of these genes was maximal ... | 2013 | 23648850 |
| maize kernel antioxidants and their potential involvement in fusarium ear rot resistance. | the potential involvement of antioxidants (α-tocopherol, lutein, zeaxanthin, β-carotene, and ferulic acid) in the resistance of maize varieties to fusarium ear rot was the focus of this study. these antioxidants were present in all maize kernel stages, indicating that the fumonisin-producing fungi (mainly fusarium verticillioides and fusarium proliferatum ) are likely to face them during ear colonization. the effect of these compounds on fumonisin biosynthesis was studied in f. verticillioides l ... | 2013 | 23484637 |
| diversity of pea-associated f. proliferatum and f. verticillioides populations revealed by fum1 sequence analysis and fumonisin biosynthesis. | fusarium proliferatum and f. verticillioides are considered as minor pathogens of pea (pisum sativum l.). both species can survive in seed material without visible disease symptoms, but still contaminating it with fumonisins. two populations of pea-derived f. proliferatum and f. verticillioides strains were subjected to fum1 sequence divergence analysis, forming a distinct group when compared to the collection strains originating from different host species. furthermore, the mycotoxigenic abilit ... | 2013 | 23470545 |
| wetting properties of fungi mycelium alter soil infiltration and soil water repellency in a γ-sterilized wettable and repellent soil. | soil water repellency (swr) has a drastic impact on soil quality resulting in reduced infiltration, increased runoff, increased leaching, reduced plant growth, and increased soil erosion. one of the causes of swr is hydrophobic fungal structures and exudates that change the soil-water relationship. the objective of this study was to determine whether swr and infiltration could be manipulated through inoculation with fungi. the effect of fungi on swr was investigated through inoculation of three ... | 2012 | 23245615 |
| host extracts induce changes in the proteome of plant pathogen fusarium proliferatum. | fusarium proliferatum is a polyphagous pathogenic fungus able to infect many crop plants worldwide. differences in proteins accumulated were observed when maize- and asparagus-derived f. proliferatum strains were exposed to host extracts prepared from asparagus, maize, garlic, and pineapple tissues. seventy-three unique proteins were up-regulated in extract-supplemented cultures compared to the controls. they were all identified using mass spectrometry and their putative functions were assigned. ... | 2017 | 28705396 |
| biosynthesis of nicotinic acid from 3-cyanopyridine by a newly isolated fusarium proliferatum zjb-09150. | in this study, nitriles were used as sole sources of nitrogen in the enrichments to isolate nitrile-converting microorganisms. a novel fungus named zjb-09150 possessing nitrile-converting enzymes was obtained with 3-cyanopyridine as sole source of nitrogen, which was identified by morphology, biology and 18s rdna gene sequence as fusarium proliferatum. it was found that f. proliferatum had ability to convert nitriles to corresponding acids or amides and showed wide substrate specificity to aliph ... | 2013 | 23085954 |
| a multiplex real-time pcr method using hybridization probes for the detection and the quantification of fusarium proliferatum, f. subglutinans, f. temperatum, and f. verticillioides. | maize contamination with fusarium species is one of the major sources of mycotoxins in food and feed derivates. in the present study, a lightcycler(®) real-time pcr method using hybridization probes was developed for the specific identification, detection, and quantification of fusarium proliferatum, fusarium subglutinans, fusarium temperatum, and fusarium verticillioides, four mycotoxin-producing pathogens of maize. primers and hybridization probes were designed to target the translation elonga ... | 2012 | 23063186 |
| the production of β-glucosidases by fusarium proliferatum nbrc109045 isolated from vietnamese forest. | fusarium proliferatum nbrc109045 is a filamentous fungus isolated from vietnamese forest due to high production of β-glucosidases. production of the enzyme was studied on varied carbon source based mediums. the highest activity was obtained in medium containing 1% corn stover + 1% wheat bran (3.31 ± 0.14 u/ml). it is interesting to note that glucose (0.69 ± 0.02 u/ml) gave higher activity and just followed by cellobiose among the di- and mono-saccharides, which is generally regarded as a univers ... | 2012 | 22974424 |
| near-infrared (nir) hyperspectral imaging and multivariate image analysis to study growth characteristics and differences between species and strains of members of the genus fusarium. | near-infrared (nir) hyperspectral imaging was used to study three strains of each of three fusarium spp. (fusarium subglutinans, fusarium proliferatum and fusarium verticillioides) inoculated on potato dextrose agar in petri dishes after either 72 or 96 h of incubation. multivariate image analysis was used for cleaning the images and for making principal component analysis (pca) score plots and score images and local partial least squares discriminant analysis (pls-da) models. the score images, ... | 2012 | 22903431 |
| corrigendum to "characterization of a new α-l-fucosidase isolated from fusarium proliferatum le1 that is regioselective to α-(1 → 4)-l-fucosidic linkage in the hydrolysis of α-l-fucobiosides" [biochimie 132c (2017) 54-65]. | | 2017 | 28434920 |
| comparison of antifungal activities of vietnamese citrus essential oils. | citrus essential oils (eos) are volatile compounds from citrus peels and widely used in perfumes, cosmetics, soaps and aromatherapy. in this study, inhibition of citrus eos extracted from vietnamese orange (citrus sinensis), mandarin (citrus reticulata blanco), pomelo (citrus grandis osbeck) and lime (citrus aurantifolia swingle) on the growth of plant pathogenic fungi, mucor hiemalis, penicillium expansum and fusarium proliferatum was investigated. the eos of the citrus peels were obtained by c ... | 2013 | 22799453 |
| antioxidant capacity, antimicrobial activity and triterpenes isolated from jatropha isabellei müll arg. | jatropha isabellei müll arg. belongs to the family euphorbiaceae. the antioxidant capacity of this plant was evaluated by the dpph method and inhibition of thiobarbituric acid reactive substances (tbars). ethyl acetate fraction demonstrated the best antioxidant capacity against the dpph free radical and its profile was similar to ascorbic acid. this fraction also presented the best results of phenolics, flavonoids and condensed tannins, showing a positive relation between the phenolic content an ... | 2013 | 22788721 |
| benefit of polyhexamethylene biguanide in fusarium keratitis. | to report a series of 3 patients with soft contact lens-related fusarium keratitis. two of them were treated with the antiamoebic polyhexamethylene biguanide 0.02% (phmb) in combination with antifungal drugs, and 1 patient was treated with phmb as sole antifungal regimen. | 2012 | 22710976 |
| segregation of secondary metabolite biosynthesis in hybrids of fusarium fujikuroi and fusarium proliferatum. | fusarium fujikuroi and fusarium proliferatum are two phylogenetically closely related species of the gibberella fujikuroi species complex (gfc). in some cases, strains of these species can cross and produce a few ascospores. in this study, we analyzed 26 single ascospore isolates of an interspecific cross between f. fujikuroi c1995 and f. proliferatum d4854 for their ability to produce four secondary metabolites: gibberellins (gas), the mycotoxins fusarin c and fumonisin b(1), and a family of re ... | 2012 | 22626844 |
| in vitro antifungal susceptibility of filamentous fungi causing rare infections: synergy testing of amphotericin b, posaconazole and anidulafungin in pairs. | mucormycetes (formerly known as zygomycetes of the order mucorales) and hyaline moulds such as those of the genus fusarium or paecilomyces are emerging as significant human pathogens. the aim of the study was to determine the in vitro antifungal susceptibility of these fungi to older and newer antifungals and to investigate the antifungal activity of amphotericin b, posaconazole and anidulafungin in dual combinations. | 2012 | 22535624 |
| combined effect of chitosan and water activity on growth and fumonisin production by fusarium verticillioides and fusarium proliferatum on maize-based media. | the objectives of the present study were to determine the in vitro efficacy of chitosan (0.5, 1.0, 2.0 and 3.0mg/ml) under different water availabilities (0.995, 0.99, 0.98, 0.96 and 0.93) at 25°c on lag phase, growth rate and fumonisin production by isolates of fusarium verticillioides and fusarium proliferatum. the presence of chitosan affected growth and fumonisin production, and this effect was dependent on the dose and aw treatment used. the presence of chitosan increased the lag phase, and ... | 2014 | 24929683 |
| the fus3/kss1-type map kinase gene fpk1 is involved in hyphal growth, conidiation and plant infection of fusarium proliferatum. | fusarium proliferatum is an important pathogen of maize that is responsible for ear rots, stalk rots and seeding blight worldwide. during the past decade, f. proliferatum has caused several severe epidemics of maize seedling blight in many areas of china, which led to significant losses in maize. to understand the molecular mechanisms in the fungal developmental regulation and pathogenicity, we isolated and characterized the fpk1 gene (genbank accession no. hq844224) encoding a map kinase homolo ... | 2011 | 22286038 |
| the role of mycelium production and a mapk-mediated immune response in the c. elegans-fusarium model system. | fusariosis is an emerging infectious complication of immune deficiency, but models to study this infection are lacking. the use of the soil nematode caenorhabditis elegans as a model host to study the pathogenesis of fusarium spp. was investigated. we observed that fusarium conidia consumed by c. elegans can cause a lethal infection and result in more than 90% killing of the host within 120 hours, and the nematode had a significantly longer survival when challenged with fusarium proliferatum com ... | 2012 | 22225407 |
| natural occurrence of toxigenic fusarium proliferatum on paddy (oryza sativa l.) in karnataka, india. | contamination of paddy seeds (rice with husk) by fusarium species can cause spoilage and subsequent production of mycotoxins, especially fumonisins that affect human and animal health. a mycological study was conducted to evaluate the natural occurrence of fumonisin b1 produced by fusarium proliferatum on paddy grown in different geographic regions of karnataka (india). a total of 65 isolates of f. proliferatum from paddy samples were analysed by polymerase chain reaction (pcr). one set of prime ... | 2010 | 24575185 |
| occurrence of fumonisins in asparagus (asparagus officinalis l.) and garlic (allium sativum l.) from germany. | fusarium proliferatum is able to produce fumonisins and is considered a pathogen of many economically important plants (e.g. corn, rice, asparagus) [1]. the occurrence of fumonisin fb1 inf. proliferatum infected asparagus spears from germany was investigated using a liquid chromatography/electrospray ionization-mass spectrometry (lc-esi-ms) method with isotopically labeled fumonisin fb1-d6 as internal standard. asparagus samples were harvested in july 2000 and screened forfusarium species. altog ... | 2004 | 23604994 |
| mating populations of fusarium section liseola from rice, sugarcane and maize. | mating studies were conducted on 78 isolates of fusarium species section liseola from rice, sugarcane and maize. from the crosses with tester strains of gibberella fujikuroi species complex, 64.1% (50 out of 78 isolates) were cross-fertile with tester strains of mating populations a to e. the results of the mating studies showed that of the 50 isolates, 19 belonged to mating population a (gibberella moniliformis), 18 to mating population b (gibberella sacchari), 4 to mating population e (gibbere ... | 2011 | 24575220 |
| the efficient production of 3β,7α,15α-trihydroxy-5-androsten-17-one from dehydroepiandrosterone by gibberella intermedia. | the biotransformation of dehydroepiandrosterone (dhea) by gibberella intermedia was investigated. the formation of the main products was monitored by high-performance liquid chromatography (hplc). products were purified and identified as 3β,7α-dihydroxy-5-androsten-17-one (7α-oh-dhea) and 3β,7α,15α-trihydroxy-5-androsten-17-one (7α,15α-dioh-dhea) using mass spectroscopy (ms) and nuclear magnetic resonance (nmr) analyses. 7α,15α-dioh-dhea is a key intermediate for the synthesis of pharmacological ... | 2014 | 25245679 |
| the endophytic fungi of salvia miltiorrhiza bge.f. alba are a potential source of natural antioxidants. | salvia miltiorrhiza bge. f. alba is a traditional chinese herbal drug with special pharmacological effect on thromboangiitis obliterans. however, the nature source of s.miltiorrhiza bge.f.alba is now in short supply because of the over-collection of the wild plant. to better utilize this resource, the diversity and antioxidant activity of endophytic fungi isolated from s. miltiorrhiza bge. f. alba were investigated. | 2015 | 28510814 |
| co-culturing of fungal strains against botrytis cinerea as a model for the induction of chemical diversity and therapeutic agents. | new fungal sms (sms) have been successfully described to be produced by means of in vitro-simulated microbial community interactions. co-culturing of fungi has proved to be an efficient way to induce cell-cell interactions that can promote the activation of cryptic pathways, frequently silent when the strains are grown in laboratory conditions. filamentous fungi represent one of the most diverse microbial groups known to produce bioactive natural products. triggering the production of novel anti ... | 2017 | 28469610 |
| pathological interrelations of soil-borne diseases in cucurbits caused by fusarium species and meloidogyne incognita. | pathological interrelations of two soil-borne diseases in cucurbits (watermelon, oriental melon, shintosa and cucumber) caused by fusarium isolates (fi) and the root-knot nematode (rkn), meloidogyne incognita were characterized by the fusarium disease severity index (di), rkn gall index (gi) and eggmass index (ei) in inoculation tests using fi and rkn. virulence of fi as determined by di at 4 weeks after inoculation was mostly in the higher order of fusarium proliferatum f6, f5 and fusarium oxys ... | 2017 | 28811758 |
| amycolatopsis acidiphila sp. nov., a moderately acidophilic species isolated from coal mine soil. | little is known on members of the genus amycolatopsis inhabiting acidic habitats. in this study, a moderately acidophilic amycolatopsis strain, designated 2-5t, was isolated from coal mine soil, and subjected to a polyphasic taxonomic characterization. analysis based on 16s rrna gene sequences indicated that the strain was most closely related to the type strain of amycolatopsis bartoniae, sharing 99.30 % similarity, while similarity to all other amycolatopsis species was less than 97 %. the dna ... | 2017 | 28857027 |
| carbon sources influence fumonisin production in fusarium proliferatum. | fusarium proliferatum is a world-wide fungal pathogen that produces fumonisins which are harmful to animal and human health. however, environmental factors affecting fumonisin biosynthesis in f. proliferatum are not well understood. based on our preliminary results, in this study, we investigated the effect of sucrose or mannose as the sole carbon source on fumonisin b (fb) production by f. proliferatum and studied their underlying mechanisms via proteome and gene expression analysis. our result ... | 2017 | 28834107 |
| skin lesion in a patient after hematopoietic stem cell transplant. | we present a case of a 61-year-old caucasian woman who was hospitalized with fevers on day 176 after a matched unrelated stem cell transplant for acute myelogenous leukemia. she developed hemorrhagic bullae on the skin of her right thigh, and blood cultures and skin biopsy both confirmed fusarium proliferatum. despite antifungal therapy, her condition worsened and she died while on comfort-only measures. this article is protected by copyright. all rights reserved. | 2017 | 28741746 |
| detection of fumonisins in fresh and dehydrated commercial garlic. | an epidemic fungal disease caused by fusarium proliferatum, responsible for fumonisin production (fb1, fb2, and fb3), has been reported in the main garlic-producing countries in recent years. fumonisins are a group of structurally related toxic metabolites produced by this pathogen. the aim of this work was to establish an enzyme-linked immunosorbent assay (elisa) procedure, mostly applied to cereals, that is suitable for fumonisin detection in garlic and compare these results to those obtained ... | 2017 | 28719747 |
| heterologous expression in pichia pastoris and biochemical characterization of the unmodified sulfatase from fusarium proliferatum le1. | sulfatases are a family of enzymes (sulfuric ester hydrolases, ec 3.1.6.-) that catalyze the hydrolysis of a wide array of sulfate esters. to date, despite the discovery of many sulfatase genes and the accumulation of data on numerous sulfated molecules, the number of characterized enzymes that are key players in sulfur metabolism remains extremely limited. while mammalian sulfatases are well studied due to their involvement in a wide range of normal and pathological biological processes, lower ... | 2017 | 28651356 |
| fusarium proliferatum - causal agent of garlic bulb rot in spain: genetic variability and mycotoxin production. | fusarium proliferatum is a world-wide occurring fungal pathogen affecting several crops included garlic bulbs. in spain, this is the most frequent pathogenic fungus associated with garlic rot during storage. moreover, f. proliferatum is an important mycotoxigenic species, producing a broad range of toxins, which may pose a risk for food safety. the aim of this study is to assess the intraspecific variability of the garlic pathogen in spain implied by analyses of translation elongation factor (te ... | 2017 | 28648292 |
| proteomics analysis of fusarium proliferatum under various initial ph during fumonisin production. | fusarium proliferatum as a fungal pathogen can produce fumonisin which causes a great threat to animal and human health. proteomic approach was a useful tool for investigation into mycotoxin biosynthesis in fungal pathogens. in this study, we analyzed the fumonisin content and mycelium proteins of fusarium proliferatum cultivated under the initial ph5 and 10. fumonisin production after 10days was significantly induced in culture condition at ph10 than ph5. ninety nine significantly differently a ... | 2017 | 28522339 |
| abiotic conditions leading to fum gene expression and fumonisin accumulation by fusarium proliferatum strains grown on a wheat-based substrate. | fusarium proliferatum produces fumonisins b not only on maize but also on diverse crops including wheat. using a wheat-based medium, the effects of abiotic factors, temperature and water activity (aw), on growth, fumonisin biosynthesis, and expression of fum genes were compared for three f. proliferatum strains isolated from durum wheat in argentina. although all isolates showed similar profiles of growth, the fumonisin production profiles were slightly different. regarding fum gene transcriptio ... | 2017 | 28463723 |
| enhanced biotransformation of dehydroepiandrosterone to 3β,7α,15α-trihydroxy-5-androsten-17-one with gibberella intermedia ca3-1 by natural oils addition. | dihydroxylation of dehydroepiandrosterone (dhea) is an essential step in the synthesis of many important pharmaceutical intermediates. however, the solution to the problem of low biohydroxylation conversion in the biotransformation of dhea has yet to be found. the effects of natural oils on the course of dihydroxylation of dhea to 3β,7α,15α-trihydroxy-5-androsten-17-one (7α,15α-dioh-dhea) were studied. with rapeseed oil (2 %, v/v) addition, the bioconversion efficiency was improved, and the 7α,1 ... | 2014 | 25145444 |
| improving the catalytic potential and substrate tolerance of gibberella intermedia nitrilase by whole-cell immobilization. | comparative studies of immobilized and free cells of gibberella intermedia ca3-1 in bioconversion of 3-cyanopyridine to nicotinic acid were performed. entrapping method was chosen based on the advantages in enzymatic activity recovery, mechanical strength and preparation procedure. four entrapment matrices were investigated and sodium alginate was screened to be the most suitable material. maximal nitrilase activity of alginate immobilized cells was obtained under conditions of 2 % alginate, 0.6 ... | 2015 | 25030338 |
| [breeding of high 3beta,7alpha,15alpha-trihydroxy-5-androsten-17-one transforming strains and their conversion process optimization]. | in order to improve transformation efficiency of dehydroepiandrosterone (dhea) into 3beta,7alpha,15alpha-trihydroxy-5-androsten-17-one (7alpha,15alpha-dioh-dhea) by gibberella intermedia ca3-1, we investigated the strains breeding and their conversion process optimization. g. intermedia ca3-1 strains were treated with 0.12 mg/ml 1-methyl-3-nitro-1-nitroso-guanidin (ntg) for 30 min and chosen by 350 micromol/l minimum inhibitory concentration ketoconazole resistance marker. the high production st ... | 2013 | 24701835 |
| [biocatalytic desymmetric hydrolysis of 3-(4-chlorophenyl)-glutaronitrile to the key precursor of optically pure baclofen]. | we produced (s)-4-cyano-3-(4-chlorophenyl)-butyrate by highly stereoselective biocatalyst in this study. a nitrilase-producing strain, named gibberella intermedia wx12, was isolated by 3-(4-chlorophenyl)-glutaronitrile as substrate in the screening with phenol-sodium hypochlorite method. the fermentation conditions and catalytic properties of this strain were investigated. the preferred carbon and nitrogen sources for nitrilase production were lactose (30 g/l) and peptone (20 g/l). after being c ... | 2013 | 23631116 |
| isolation and characterization of gibberella intermedia ca3-1, a novel and versatile nitrilase-producing fungus. | nitrilase-mediated biocatalysis has attracted substantial attention for its application in carboxylic acid production in recent years. in the present study, the fungus ca3-1 was isolated and identified as gibberella intermedia based on its morphology, its 18s ribosomal dna (rdna), and internal transcribed spacer (its) sequences. the enzymatic properties of g. intermedia resting cells were determined, and the optimum activity was achieved at 40 °c with ph 7.6. the half-lives of the nitrilase at 3 ... | 2013 | 23457057 |
| analysis of fumonisin contamination and the presence of fusarium in wheat with kernel black point disease in the united states. | the ability of the fungus fusarium proliferatum to cause kernel black point disease in wheat was previously established, but natural contamination of black point wheat with both f. proliferatum and fumonisin mycotoxins has not been studied in the united states. low levels of fumonisins were detected in nine of 43 wheat samples with kernel black point disease that were obtained from across the united states. a subset of samples was contaminated with f. proliferatum as well as with f. fujikuroi, f ... | 2012 | 22494515 |