| mould germination: data treatment and modelling. | the objectives of this study were i/ to examine germination data sets over a range of environmental conditions (water activity, temperature) for eight food spoilage moulds, ii/ to compare the ability of the gompertz equation and logistic function to fit the experimental plots, iii/ to simulate germination by assessing various distributions of the latent period for germination amongst a population of spores. data sets (percentage germination, p (%), versus time, t) of aspergillus carbonarius, asp ... | 2007 | 17188772 |
| fungi recovered from slovakian poultry feed mixtures and their toxinogenity. | to contribute towards the knowledge of microbiology of feeds, more than 100 samples of poultry feed mixtures from slovakia were mycologically investigated in terms of the overall fungal diversity and toxicological potential of isolated fungi. the study revealed that out of 22 genera recovered, penicillium was the most frequent and diverse genus, followed by aspergillus and mucor being found in 89 % (34 spp.), 69 % (11 spp.) and 50 % (4 spp.), respectively. the most frequently encountered taxa we ... | 2006 | 17195991 |
| contamination of pine nuts by fumonisin produced by strains of fusarium proliferatum isolated from pinus pinea. | to test the ability of fusarium proliferatum strains isolated from pinus pinea to synthesize fumonisin b(1) (fb(1)) in pine nuts. | 2007 | 17209817 |
| biodiversity of fusarium species in mexico associated with ear rot in maize, and their identification using a phylogenetic approach. | fusarium proliferatum, f. subglutinans, and f. verticillioides are known causes of ear and kernel rot in maize worldwide. in mexico, only f. verticillioides and f. subglutinans, have been reported previously as causal agents of this disease. however, fusarium isolates with different morphological characteristics to the species that are known to cause this disease were obtained in the highland-valley region of this country from symptomatic and symptomless ears of native and commercial maize genot ... | 2007 | 17216329 |
| cloning and characterization of fpmtr1, an amino acid transporter gene of fusarium proliferatum (gibberella intermedia). | fpmtr1, an amino acid transporter gene from fusarium proliferatum was strongly expressed during conidial germination and repressed in late stationary phase. to identify the specific function of this gene, deltafpmtr1 knock-out mutants were generated by gene replacement. vegetative growth of the deltafpmtr1 mutants was normal both in liquid and on solid media, but conidial germination was delayed. the deltafpmtr1 mutants and the wild type were equally fertile when used as males in sexual crosses, ... | 2007 | 17304613 |
| occurrence of fumonisins b1 and b2 in portuguese maize and maize-based foods intended for human consumption. | fumonisin b(1) (fb(1)) and fumonisin b(2) (fb(2)) are mycotoxins mainly produced by fusarium verticillioides and fusarium proliferatum, which are field pathogens of maize. a survey was conducted on the incidences of fb(1) and fb(2) in both maize and derived products purchased in portugal. the analytical method involved extraction with methanol-water, clean-up by immunoaffinity column and derivatization with naphthalene-2,3-dicarboxaldehyde. determination was carried out by high-performance liqui ... | 2007 | 17454111 |
| isolation and properties of a levo-lactonase from fusarium proliferatum ecu2002: a robust biocatalyst for production of chiral lactones. | a fungus strain ecu2002, capable of enantioselectively hydrolyzing chiral lactones to optically pure hydroxy acids, was newly isolated from soil samples through two steps of screening and identified as fusarium proliferatum (matsushima) nirenberg. from the crude extract of f. proliferatum ecu2002, a novel levo-lactonase was purified to homogeneity, with a purification factor of 460-folds and an overall yield of 9.7%, by ultrafiltration, acetone precipitation, and chromatographic separation throu ... | 2007 | 17530243 |
| occurrence of fumonisins b1 and b2 in broa, typical portuguese maize bread. | fumonisin b(1) (fb(1)) and fumonisin b(2) (fb(2)) are mycotoxins mainly produced by fusarium verticillioides, and fusarium proliferatum, fungi species most commonly isolated from maize. the natural occurrence of fb(1) and fb(2) in broa, typical portuguese maize bread, was evaluated in 30 samples. twenty five were found positive with levels ranging from 142 to 550 microg kg(-1). the limit established by the european regulations was exceeded by 27% of the samples. the tolerable daily intake for fu ... | 2007 | 17604863 |
| fumonisins production by fusarium proliferatum strains isolated from asparagus crown. | the present work deals with the capability for producing fumonisin by fusarium proliferatum strains isolated from asparagus in china. fifty of f. proliferatum strains were randomly selected and incubated on cultures of maize grain and asparagus spear, respectively. fumonisin levels (fb(1) and fb(2)) were determined by high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (hplc-esi-ms/ms). the results showed that all 50 strains produced fumonisins in m ... | 2007 | 17629787 |
| genetic factors affecting sexual reproduction in toxigenic fusarium species. | mycotoxin producing capability greatly varies within species. in theory, the major source of this variability is meiotic recombination. however, a number of important toxigenic species have no known sexual stage and, therefore, the origin of the intraspecific diversity in these fungi is poorly understood. mating in sexually reproducing ascomycetes is controlled by mat genes, but fungi with no known sexual stage also may have fully functional, constitutively transcribed mating type genes. the mat ... | 2007 | 17720271 |
| wheat kernel black point and fumonisin contamination by fusarium proliferatum. | fusarium proliferatum is a major cause of maize ear rot and fumonisin contamination and also can cause wheat kernel black point disease. the primary objective of this study was to determine whether nine f. proliferatum strains from wheat from nepal can cause black point and fumonisin contamination in wheat kernels. for comparison, the study included three fusarium strains from us maize. in test 1, all the strains but one produced significant symptoms of kernel black point; two strains decreased ... | 2007 | 17886185 |
| antifungal activity of lactobacillus paracasei ssp. tolerans isolated from a sourdough bread culture. | lactic acid bacteria were isolated from four different sourdough bread cultures previously investigated for antifungal activity. a total of 116 isolates were obtained and screened for antifungal activity against a battery of molds. the most inhibitory isolate obtained was identified by api 50 chl and 16s ribosomal rna genotyping and found to be lactobacillus paracasei ssp. tolerans. this isolate completely inhibited the growth of fusarium proliferatum m 5689, m 5991 and fusarium graminearum r 40 ... | 2008 | 18077044 |
| successful salvage treatment of disseminated cutaneous fusariosis with liposomal amphotericin b and terbinafine after allogeneic stem cell transplantation. | fusarium infections are associated with high mortality after allogeneic stem cell transplantation. we report on successful treatment of a disseminated cutaneous fusarium proliferatum infection using liposomal amphotericin b and terbinafine. in vitro susceptibility tests of antifungal drugs suggest that terbinafine is a potent additional antifungal drug for disseminated cutaneous fusariosis. | 2008 | 18194367 |
| characterization of a new mitochondrial plasmid from fusarium proliferatum. | a 10.3kb linear mitochondrial dna plasmid designated pfp1 was isolated from fusarium proliferatum. the dna sequence of the plasmid consists of 10,336bp with perfect terminal inverted repeats of 400bp. two major, non-overlapping orfs were identified on opposite strands, encoding a phage-type rna polymerase and a family b type dna polymerase, respectively. one additional minor orf encoding a putative highly basic protein was also identified. the copy number of pfp1, as determined by rt-pcr, ranged ... | 2008 | 18262647 |
| antifungal susceptibility profile of clinical fusarium spp. isolates identified by molecular methods. | to analyse the susceptibility pattern of a collection of fusarium clinical isolates. | 2008 | 18263569 |
| a fumonisin biosynthetic gene cluster in fusarium oxysporum strain o-1890 and the genetic basis for b versus c fumonisin production. | most species of fusarium that produce fumonisin mycotoxins produce predominantly b fumonisins (fbs). however, fusarium oxysporum strain o-1890 produces predominantly c fumonisins (fcs). in this study, the nucleotide sequence of the fumonisin biosynthetic gene (fum) cluster in strain o-1890 was determined. the order and orientation of fum genes were the same as in the previously described clusters in fusarium verticillioides and fusarium proliferatum. coding regions of f. oxysporum and f. vertici ... | 2008 | 18375156 |
| fphog1, a hog-type map kinase gene, is involved in multistress response in fusarium proliferatum. | delta fphog1 mutants of fusarium proliferatum obtained by targeted gene disruption of fphog1, an orthologue of the saccharomyces cerevisiae hog1 mapk gene showed increased sensitivity towards different abiotic stressors including uv-irradiation, heat, salt, osmotic and hydrogen peroxide treatments. incubation of the delta fphog1 mutants under hyperosmotic conditions was accompanied with prolonged growth arrest, inhibition of conidial germination, morphological abnormalities and time-dependent in ... | 2008 | 18506895 |
| the use of its dna sequence analysis and maldi-tof mass spectrometry in diagnosing an infection with fusarium proliferatum. | although mycoses are among the most common diseases worldwide, infections with fusarium spp. occur only rarely. mostly patients suffering from underlying immune deficiency are infected with this mould, resulting in a considerably decreasing prognosis. in immunocompromised patients, cutaneous manifestations are more often associated with fusarium sp. than with candida sp. or aspergillus sp. we describe one patient with acute lymphoblastic leukaemia, who was first treated with chemotherapy after g ... | 2008 | 18547323 |
| metabiotic effects of fusarium spp. on escherichia coli o157:h7 and listeria monocytogenes on raw portioned tomatoes. | the metabiotic effects of fusarium proliferatum, f. avenaceum, and f. oxysporum on escherichia coli o157:h7 and listeria monocytogenes in fresh tomatoes were investigated. tomatoes were preinoculated with the molds and incubated at 15 degrees c for 7 days; then they were inoculated separately with the pathogens, packaged in air and modified atmosphere (5% o2, 30% co2, and 65% n2), and stored at 4, 8, and 12 degrees c for 9 days. the cell loads of pathogens and lactic acid bacteria and the ph wer ... | 2008 | 18680934 |
| production of gibberellic acids by an orchid-associated fusarium proliferatum strain. | the rice pathogen fusarium fujikuroi is well known for its ability to produce the plant hormones gibberellins (gas). however, the majority of closely related fusarium species is unable to produce gas although the ga gene cluster is present in their genomes. in this study, we analyzed five orchid-associated fusarium isolates for their capacity to produce gas. four of them did not produce any gas and were shown not to contain any ga biosynthetic genes. however, the fifth isolate, which has been id ... | 2008 | 18694840 |
| mango malformation disease and the associated fusarium species. | abstract mango malformation disease (mmd) occurs in asia, africa, and the americas and was first reported in india in 1891. the vegetative form of mmd was first reproduced in 1966 with fusarium moniliforme and the floral form with isolates of f. moniliforme var. subglutinans from both vegetative shoots and floral tissue. the fungi were subsequently recognized as f. subglutinans. in 2002, a new species, f. mangiferae, was established based on nuclear and mitochondrial dna sequences; it included s ... | 2006 | 18943188 |
| isolation and characterization of a cold-tolerant strain of fusarium proliferatum, a biocontrol agent of grape downy mildew. | abstract a cold-tolerant strain of the mycoparasite fusarium proliferatum was isolated following uv mutagenesis of the g6 strain, which is a biocontrol agent of grape downy mildew. the isolated strain (designated 1505) exhibited radial growth two to threefold that of the parent strain when grown at 13 degrees c, which is generally suboptimal for growth of fusarium spp., but desirable for its host, plasmopara viticola. this rapid growth was correlated with improved biological control of p. vitico ... | 2001 | 18943441 |
| antifungal activity of lactobacillus paracasei subsp. tolerans against fusarium proliferatum and fusarium graminearum in a liquid culture setting. | lactobacillus paracasei subsp. tolerans, isolated from a traditional sourdough bread culture and previously shown to have antifungal activity against fusarium species, was tested for inhibition of growth of fusarium proliferatum m 5991 and m 5689 and f. graminearum r 4053 in a liquid medium setting. this isolate completely inhibited the growth of f. proliferatum m 5689 and m 5991 and f. graminearum r 4053, whereas such growth was not inhibited in the control in a supernatant agar plate assay. wh ... | 2008 | 19044263 |
| synergistic degradation of 2-naphthol by fusarium proliferatum and bacillus subtilis in wastewater. | 2-naphthol, which originates widely from various industrial activities, is toxic and thus harmful to human liver and kidney. a new compound biodegradation system was adopted to degrade 2-naphthol-contaminated wastewater. enzymatic response to 2-naphthol biodegradation in the aqueous phase was also studied. as a co-metabolic substrate, salicylic acid could induce the two microorganisms to produce a large amount of degradation enzymes for 2-naphthol. the key enzymes were confirmed as polyphenol ox ... | 2009 | 19070430 |
| n-starvation stress induced fum gene expression and fumonisin production is mediated via the hog-type mapk pathway in fusarium proliferatum. | during cultivation of a wild type strain of fusarium proliferatum on ammonium dihydrogen phosphate containing defined medium, expression levels of fum1 and fum8, members of the fumonisin biosynthesis gene cluster significantly increased when ammonium ion concentration of the culture medium decreased below 10 mm, indicating that n-depletion triggers the fumonisin biosynthesis genes. deletion of fphog1, a hog-type map kinase gene resulted in further increases in fum1 and fum8 expression under nitr ... | 2009 | 19181411 |
| less-frequent fusarium species of clinical interest: correlation between morphological and molecular identification and antifungal susceptibility. | forty-eight fusarium isolates morphologically identified as belonging to seven species of clinical interest (i.e., fusarium chlamydosporum, fusarium dimerum, fusarium incarnatum, fusarium napiforme, fusarium nygamai, fusarium proliferatum, and fusarium sacchari) were characterized molecularly by the analysis of the sequences of the tub region of the beta-tubulin gene. f. chlamydosporum and f. dimerum were the most genetically heterogeneous species. a high degree of correlation between the morpho ... | 2009 | 19321723 |
| fumonisins, trichothecenes and zearalenone in cereals. | fumonisins are phytotoxic mycotoxins which are synthesized by various species of the fungal genus fusarium such as fusarium verticillioides (sacc.) nirenberg (ex f.moniliforme sheldon) and fusarium proliferatum. the trichothecene (tc) mycotoxins are secondary metabolites produce by species that belong to several fungal genera, especially fusarium, stachybotrys, trichothecium, trichoderma, memnoniella and myrothecium. fusarium mycotoxins are widely dispersed in cereals and their products. zearale ... | 2008 | 19330061 |
| fumonisin b(1) production by fusarium proliferatum strains isolated from allium fistulosum plants and seeds in japan. | to test the fumonisin b(1)-producing ability of fusarium proliferatum strains isolated from welsh onion (allium fistulosum) plants and seeds of commercial cultivars in japan and to examine the applicability of pcr-based assays to discriminate between fumonisin b(1)-producing and nonproducing isolates. | 2009 | 19416462 |
| use of mass spectrometry to identify clinical fusarium isolates. | fusarium spp. have recently emerged as significant human pathogens. identification of these species is important, both for epidemiological purposes and for patient management, but conventional identification based on morphological traits is hindered by major phenotypic polymorphism. in this study, 62 strains, or isolates, belonging to nine fusarium species were subjected to both molecular identification tef1 gene sequencing and matrix-assisted laser desorption ionization-time-of-flight (maldi-to ... | 2009 | 19456834 |
| antifungal activity, mechanism and qsar studies on chalcones. | in this paper, the synthesis of 48 chalcones and their in vitro antifungal activity against aspergillus flavus ncim 594, fusarium proliferatum ncim1105, aspergillus niger ncim 596 and candida tropicalis ncim 3556 are reported. predominantly, the active compounds (6, 9, 14, 27, and 47) against all four fungi have found to have electron withdrawing substitutions in their para substitution in b-ring. quantitative structure-activity relationship indicated that the antifungal activities are correlate ... | 2009 | 19519746 |
| the its region as a taxonomic discriminator between fusarium verticillioides and fusarium proliferatum. | the maize pathogens fusarium verticillioides (fv) and fusarium proliferatum (fp) are morphologically very similar to one another, so fp isolates have been often mistaken as fusarium moniliforme (the former name of fv). the only presently accepted morphological discriminator between these species is the presence/absence of polyphialides. here, a collection of 100 fusarium strains, isolated from infected maize kernels on plants grown in north-western italy, were assigned as fv or fp on the basis o ... | 2009 | 19631741 |
| electrochemical and afm characterization on gold and carbon electrodes of a high redox potential laccase from fusarium proliferatum. | the redox potential of the t1 copper site of laccase from fusarium proliferatum was determined by titration to be about 510 mv vs. sce (750 mv vs. nhe), which makes it a high redox potential enzyme. anaerobic electron transfer reactions between laccase and carbon and gold electrodes were detected, both in solution and when the enzyme was adsorbed on these surfaces. in solution, a single high-potential signal (660 mv vs. sce) was recorded at the carbon surfaces, attributable to the t1 copper site ... | 2010 | 19854115 |
| biocatalytic properties of a recombinant fusarium proliferatum lactonase with significantly enhanced production by optimal expression in escherichia coli. | the levo-lactonase gene of fusarium proliferatum ecu2002 (ec3.1.1.25) was cloned and expressed in escherichia coli jm109 (de3) for biocatalytic resolution of industrially important chiral lactones, including dl-pantoyl lactone which was a key precursor to calcium d-pantothenate. by increasing the biomass concentration and lowering the inducer (isopropyl-beta-d-thiogalactoside) concentration and induction temperature, the lactonase production was significantly enhanced up to 20 ku/l, which was 20 ... | 2010 | 19876606 |
| synthesis of n-alkyl-beta-d-glucosylamines and their antimicrobial activity against fusarium proliferatum, salmonella typhimurium, and listeria innocua. | in this study, different n-alkyl-beta-d-glucosylamines were evaluated for both antifungal and antibacterial activity against fusarium proliferatum (inra, mucl 1807.7), listeria innocua (istab, université bordeaux 1), and salmonella typhimurium (institut pasteur 5858). the tested glucosylamines were beta-d-glucosylamine (gpa), n-ethyl-beta-d-glucosylamine (etgpa), n-butyl-beta-d-glucosylamine (bugpa), n-hexyl-beta-d-glucosylamine (hegpa), n-octyl-beta-d-glucosylamine (ocgpa), n-dodecyl-beta-d-glu ... | 2009 | 19908897 |
| genetic variability and fumonisin production by fusarium proliferatum. | fusarium proliferatum is together with fusarium verticillioides the main source of fumonisins, a health risk mycotoxin, contaminating agro-products. contrary to f. verticillioides, it colonizes a wide range of host plants besides maize, such as wheat or barley among others, in particular in certain regions (southern europe). the phylogenetic study performed in this work using a wide sample of isolates from diverse hosts and origins revealed a high variability, while no host preferences could be ... | 2010 | 19913692 |
| fusarium proliferatum soft tissue infection at the site of a puncture by a plant: recovery, isolation, and direct molecular identification. | after allogeneic stem cell transplantation, a 49-year-old man developed fever and inflammation at the site of a plant puncture on a finger. a hyalohyphomycete was recovered by incubating the plant spine fragment following surgery. amplification of the internal transcribed spacer region and 5.8s rrna, beta-tubulin, and translation elongation factor coding genes identified fusarium proliferatum, which was confirmed later by culture. | 2010 | 19923491 |
| adenylyl cyclase regulates heavy metal sensitivity, bikaverin production and plant tissue colonization in fusarium proliferatum. | a homologue of the adenylyl cyclase (ac) gene of neurospora crassa, named fpacy1 was cloned from the genomic library of fusarium proliferatum item 2287 by screening the library with a dna fragment amplified by using pcr primers designed from conserved sequences of the catalytic domain of ac genes from other fungi. the deduced fpacy1 protein had 53-77% identity with the ac proteins of other fungi. deltafpacy1 mutants obtained by targeted gene disruption showed retarded vegetative growth, increase ... | 2010 | 20082366 |
| time-lapse analysis of cell death in mammalian and fungal cells. | time-lapse video microscopy was designed to follow the movement of single cells for an unlimited period of time under physiological conditions. the system is based on two inverted microscopes located in a co(2) incubator and equipped with charge-coupled device cameras connected to the computer. frames were recorded every minute and the subsequent video sequence was converted to database form. the system was applied to describe the movements of normal hacat cells and pb-treated cells causing the ... | 2010 | 20218896 |
| antibacterial effect of the bioactive compound beauvericin produced by fusarium proliferatum on solid medium of wheat. | to obtain the bioactive compound beauvericin (bea), fusarium proliferatum cect 20569 was grown on a solid medium of wheat, utilizing the technique of the solid state fermentation (ssf), being this mycotoxin purified by high performance liquid chromatography (hplc) with a reverse phase semi-preparative column using as the mobile phase acetonitrile/water in gradient condition. the purity of the bea was verified by analytical hplc and liquid chromatography tandem mass spectrometry (lc/ms-ms). the p ... | 2010 | 20371252 |
| antifungal effects of the bioactive compounds enniatins a, a(1), b, b(1). | to produce enniatin (ens), fusarium tricinctum cect 20150 was grown in a liquid medium of potato (pdb), being mycotoxin purified by high performance liquid chromatography (hplc) with a reverse phase semipreparative column using a mobile phase of acetonitrile/water using gradient condition. the purity of the ens fractions was verified by analytical hplc and lc/ms-ms. the pure fractions of ens were utilized to study the biological activity on several mycotoxigenic moulds as fusarium verticilloides ... | 2010 | 20417654 |
| formation of fumonisins and other secondary metabolites by fusarium oxysporum and f. proliferatum: a comparative study. | the principal aim of this study was to estimate the formation of fumonisins (fb(1) and fb(2)), moniliformin (mon), and ergosterol (erg) by fusarium oxysporum and fusarium proliferatum, while the formation of beauvericin (bea) was estimated by the latter fusarium species only. moreover, the effect of temperature on the biosynthesis of mycotoxins was also evaluated. fumonisins were formed by f. proliferatum, with the highest yield at 18 degrees c (720.0-1976.6 microg g(-1) for fb(1), 74.2-670.8 mi ... | 2010 | 20455157 |
| characterization of glycolipid biosurfactant from pseudomonas aeruginosa cpcl isolated from petroleum-contaminated soil. | to isolate and characterize the biosurfactant-producing micro-organism from petroleum-contaminated soil as well as to determine the biochemical properties of the biosurfactant. | 2010 | 20477962 |
| differential effect of environmental conditions on the growth and regulation of the fumonisin biosynthetic gene fum1 in the maize pathogens and fumonisin producers fusarium verticillioides and fusarium proliferatum. | the effects of ecophysiological factors, temperature and solute potential, on both the growth and the regulation of the fumonisin biosynthetic fum1 gene were studied and compared in one isolate each of the two closely related fumonisin-producing and maize pathogens fusarium verticillioides and fusarium proliferatum. the effect of solute potential and temperature was examined on in vitro mycelia growth and on the expression of the fum1 gene, quantified by species-specific real-time reverse transc ... | 2010 | 20491926 |
| potential of pediococcus pentosaceus (l006) isolated from maize leaf to suppress fumonisin-producing fungal growth. | the present study was aimed at characterizing the ability of lactic acid bacteria isolated from maize to repress the growth of fumonisin-producing fungi. a total of 67 isolates were screened for their antifungal activity against fusarium proliferatum and fusarium verticillioides by using the overlay method. the most efficient antifungal isolate was identified as pediococcus pentosaceus (l006), on the basis of physiological and biochemical characterization and 16s rrna gene sequencing. production ... | 2010 | 20537273 |
| hydroxylation at carbon-2 of ent-16-oxo-17-norkauran-19-oic acid by fusarium proliferatum. | a new product of biotransformation of ent-16-oxo-17-norkauran-19-oic acid (1) by fusarium proliferatum was isolated and identified as a 2beta-hydroxy derivative (2). the structure of 2 was elucidated on the basis of spectroscopic data interpretation and single-crystal x-ray diffraction analysis. the allelopathic activity of compound 2 was evaluated on the growth of radicals and shoots of lactuca sativa (lettuce). this is the first time that fungal hydroxylation at position c-2 has been reported ... | 2010 | 20617817 |
| fumonisin b(1) and implications in nursery swine productivity: a quantitative exposure assessment. | this study estimated the long-term exposure of fumonisin b(1) (fb(1)) in nursery swine diets and associated toxicological adverse effects on negative productivity potential using quantitative exposure assessment. fumonisin b(1) is a mycotoxin produced by fusarium verticillioides and fusarium proliferatum and is a common biological contaminant of corn (zea mays l.) and other grains. acute effects from fb(1) exposures are well recognized and managed in the swine industry, but practices to limit pr ... | 2010 | 20622179 |
| production and migration of mycotoxins in sweet pepper analyzed by multimycotoxin lc-ms/ms. | in this work the presence and migration behavior of mycotoxins formed in sweet pepper, inoculated by fusarium species involved in internal fruit rot, were investigated. two different commercial sweet pepper cultivars were inoculated with two different fusarium proliferatum isolates that were sampled from diseased peppers. after 10 days of incubation at 20 °c in a closed container, the lesion caused by the fungal infection was dissected. around the lesion, up to three concentric rings of pepper f ... | 2010 | 20825158 |
| a molecular based strategy for rapid diagnosis of toxigenic fusarium species associated to cereal grains from argentina. | fusarium species are worldwide causal agents of ear rot in cereals. their toxigenic potential is a health risk for both humans and animals. in argentina, most identification of these fungi has been based on morphological and cross-fertility criteria which are time consuming and require considerable expertise in fusarium taxonomy and physiology. dna based approaches have been reported as rapid, sensitive and specific alternatives to identify the main fumonisin and trichothecene-producing fusarium ... | 2010 | 20965064 |
| dermatitis in captive wyoming toads (bufo baxteri) associated with fusarium spp. | from may 2007 to june 2008, 30 of 49 wyoming toads (bufo baxteri) kept at omaha's henry doorly zoo (nebraska, usa) died showing clinical signs of ventral erythema, inappetance, lethargy, and delayed righting reflex. treatment with antifungals and antibiotics was unsuccessful in all cases. histopathologic analyses revealed dermatitis as the primary problem in 20 of 21 toads in which skin was examined. fungal dermatitis was present in 17 toads, with hyphae approximately 1-3 μm in diameter, and par ... | 2010 | 20966269 |
| sphaerodes mycoparasitica biotrophic mycoparasite of 3-acetyldeoxynivalenol- and 15-acetyldeoxynivalenol-producing toxigenic fusarium graminearum chemotypes. | fusarium spp. are economically important crop pathogens and causal agents of fusarium head blight (fhb) of cereals worldwide. of the fhb pathogens, fusarium graminearum 3-acetyldeoxynivalenol (3-adon) and 15-acetyldeoxynivalenol (15-adon) are the most aggressive mycotoxigenic chemotypes, threatening food and feed quality as well as animal and human health. the objective of the study was to evaluate host specificity and fungal-fungal interactions of sphaerodes mycoparasitica- a recently described ... | 2011 | 21204935 |
| in vitro inhibitory effect of tetrahydrocurcuminoids on fusarium proliferatum growth and fumonisin b₁ biosynthesis. | many plant pathogens produce toxic metabolites when growing on food and feed. some antioxidative components seem to prevent fungal growth and mycotoxin formation. recently, we synthesized a new class of powerful antioxidative compounds, i.e. tetrahydrocurcuminoids, and its structure/antioxidant activity relationships have been established. the south west of france produces large amounts of corn, which can be infected by fusarium species, particularly f. proliferatum. in this context, the efficie ... | 2011 | 21240822 |
| fum cluster divergence in fumonisins-producing fusarium species. | fumonisins are polyketide-derived mycotoxins, produced by several fusarium species, and its biosynthetic pathway is controlled by the fum cluster--a group of genes exhibiting a common expression pattern during fumonisin biosynthesis. the most common are the b analogues with fumonisin b(1) (fb(1)) being the most prevalent. at least a part of the inter- and intraspecific variation in fbs synthesis level can be explained by the sequence differences inside fum cluster. the aim of our study was to ev ... | 2010 | 21315309 |
| production of dragon's blood in dracaena cochinchinensis plants by inoculation of fusarium proliferatum. | dragon's blood is a traditional medicine widely used in the world from ancient times. however, little is known about its formation mechanism. this work aimed to gain some insights into its formation mechanism and to control its production. the results demonstrate that wounding plus causal fungal infection and keeping the wound moist are essential for efficient dragon's blood formation in dracaena cochinchinensis. two fungal isolates ym-266 and ym-71213 of fusarium proliferatum increased the yiel ... | 2011 | 21421373 |
| determination of the loq in real-time pcr by receiver operating characteristic curve analysis: application to qpcr assays for fusarium verticillioides and f. proliferatum. | real-time pcr (qpcr) is the principal technique for the quantification of pathogen biomass in host tissue, yet no generic methods exist for the determination of the limit of quantification (loq) and the limit of detection (lod) in qpcr. we suggest using the youden index in the context of the receiver operating characteristic (roc) curve analysis for this purpose. the loq was defined as the amount of target dna that maximizes the sum of sensitivity and specificity. the lod was defined as the lowe ... | 2011 | 21603916 |
| persistent fingernail onychomycosis caused by fusarium proliferatum in a healthy woman. | | 2011 | 21668519 |
| in vitro antimicrobial and antioxidant activity of acetone and methanol extracts from thymus leucotrichius (lamiaceae). | thymus species has been used as tonic and herbal tea, antiseptic, antitussive, carminative, antioxidant, anti-inflammatory and antimicrobial activities. the acetone and methanol extracts of thymus (t.) leucotrichius (labiatae/lamiaceae) was examined for antimicrobial and antioxidant properties. | 2011 | 21796869 |
| genetic and phenotypic variation of fusarium proliferatum isolates from different host species. | fusarium proliferatum (matsushima) nirenberg is a common pathogen infecting numerous crop plants and occurring in various climatic zones. it produces large amounts of fumonisins, a group of polyketide-derived mycotoxins. fumonisin biosynthesis is determined by the presence and activity of the fum cluster, several co-regulated genes with a common expression pattern. in the present work, we analyzed 38 f. proliferatum isolates from different host plant species, demonstrating host-specific polymorp ... | 2011 | 21796391 |
| disruption of sphingolipid metabolism and induction of equine leukoencephalomalacia by fusarium proliferatum culture material containing fumonisin b(2) or b(3). | fumonisin b(1), b(2), and b(3) are inhibitors of ceramide synthase, a key enzyme in the pathway for de novo sphingolipid biosynthesis. corn, naturally contaminated with either predominantly fumonisin b(1) or pure fumonisin b(1), has been shown to cause equine leukoencephalomalacia (elem). it has been hypothesized that fumonisin-induced disruption of sphingolipid metabolism is an early event in the development of elem. recently, it was shown that fusarium proliferatum corn culture diets containin ... | 1997 | 21781781 |
| free radicals, salicylic acid and mycotoxins in asparagus after inoculation with fusarium proliferatum and f. oxysporum. | electron paramagnetic resonance was used to monitor free radicals and paramagnetic species like fe, mn, cu generation, stability and status in asparagus officinalis infected by common pathogens fusarium proliferatum and f. oxysporum. occurrence of f. proliferatum and f. oxysporum, level of free radicals and other paramagnetic species, as well as salicylic acid and mycotoxins content in roots and stems of seedlings were estimated on the second and fourth week after inoculation. in the first term ... | 2011 | 21957331 |
| Fusarium proliferatum, an endophytic fungus from Dysoxylum binectariferum Hook.f, produces rohitukine, a chromane alkaloid possessing anti-cancer activity. | Rohitukine is a chromane alkaloid possessing anti-inflammatory, anti-cancer and immuno-modulatory properties. The compound was first reported from Amoora rohituka (Meliaceae) and later from Dysoxylum binectariferum (Meliaceae) and Schumanniophyton problematicum (Rubiaceae). Flavopiridol, a semi-synthetic derivative of rohitukine is a potent CDK inhibitor and is currently in Phase III clinical trials. In this study, the isolation of an endophytic fungus, Fusarium proliferatum (MTCC 9690) from the ... | 2011 | 21898150 |
| influence of light on growth, fumonisin biosynthesis and fum1 gene expression by fusarium proliferatum. | fumonisins are a group of mycotoxins, mainly found in maize and maize-based food and feed, associated with several diseases in animals. the impact of these toxins on the economy and health worldwide has driven several efforts to clarify the role of environmental factors that can influence fumonisin biosynthesis by the toxigenic species. we analyzed the influence of light of varying wavelength on growth and fumonisin biosynthesis by the fungus fusarium proliferatum item 1719. light in general had ... | 2011 | 22137249 |
| identification and functional characterization of indole-3-acetamide-mediated iaa biosynthesis in plant-associated fusarium species. | the plant hormone indole-3-acetic acid (iaa) can be synthesized from tryptophan via the intermediate indole-3-acetamide (iam). the two genes, iaam (encoding tryptophan monooxygenase) and iaah (encoding indole-3-acetamide hydrolase) that constitute the iam pathway have been described in plant-associated bacteria. we have identified putative homologs of the bacterial iaam and iaah genes in four fusarium species -fusarium proliferatum, fusarium verticillioides, fusarium fujikuroi, and fusarium oxys ... | 2011 | 22079545 |
| a luminescent bacterium assay of fusaric acid produced by fusarium proliferatum from banana. | fusarium proliferatum was isolated as a major pathogen causing the fusarium disease in harvested banana fruit. one of its major compounds, fusaric acid, was identified by high-performance liquid chromatography-electrospray ionization mass spectrometry (hplc-esi-ms). because the light intensity of the luminescent bacterium vibrio qinghaiensis sp. nov. q67 can be inhibited by fusaric acid, the fusaric acid content of f. proliferatum was assessed and compared by both the hplc and luminescent bacter ... | 2012 | 22105299 |
| in vitro antifungal activity of terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol against mycotoxigenic plant pathogens. | the aim of this study was to examine the effect of five naturally occurring compounds from essential oils on 10 different species of mycotoxigenic fungi involved in several plant diseases. the antifungal activities of terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol were observed in vitro on fusarium subglutinans, fusarium cerealis, fusarium verticillioides, fusarium proliferatum, fusarium oxysporum, fusarium sporotrichioides, aspergillus tubingensis, aspergillus carbonarius, ... | 2011 | 22257275 |
| production of enniatins a, a1, b, b1, b4, j1 by fusarium tricinctum in solid corn culture: structural analysis and effects on mitochondrial respiration. | enniatins (ens) are secondary fungal metabolites with hexadepsipeptidic chemical structure and they possess a number of potent biological activities that can contaminate several kind of food and foodstuffs increasing the exposure risk for consumers. ens are produced by several fusariun strains including fusarium subglutinans, fusarium proliferatum and fusarium tricinctum. production of a mixture of ens was performed by culturing f. tricinctum item 9496 on white corn as substrate. the solid cultu ... | 2012 | 23692767 |
| fungal his-tagged nitrilase from gibberella intermedia: gene cloning, heterologous expression and biochemical properties. | nitrilase is an important member of the nitrilase superfamiliy. it has attracted substantial interest from academia and industry for its function of converting nitriles directly into the corresponding carboxylic acids in recent years. thus nitrilase has played a crucial role in production of commercial carboxylic acids in chemical industry and detoxification of nitrile-contaminated wastes. however, conventional studies mainly focused on the bacterial nitrilase and the potential of fungal nitrila ... | 2012 | 23226336 |
| detection of antibiotic-resistant bacteria endowed with antimicrobial activity from a freshwater lake and their phylogenetic affiliation. | antimicrobial resistance poses a serious challenge to global public health. in this study, fifty bacterial strains were isolated from the sediments of a freshwater lake and were screened for antibiotic resistance. out of fifty isolates, thirty-three isolates showed resistance against at least two of the selected antibiotics. analysis of 16s rdna sequencing revealed that the isolates belonged to ten different genera, namely staphylococcus(n = 8), bacillus(n = 7), lysinibacillus(n = 4), achromobac ... | 2016 | 27330861 |
| agrobacterium-mediated transformation of fusarium proliferatum. | fusarium proliferatum is an important pathogen that is associated with plant diseases and primarily affects aerial plant parts by producing different mycotoxins, which are toxic to humans and animals. within the last decade, this fungus has also been described as one of the causes of red root rot or sudden death syndrome in soybean, which causes extensive damage to this crop. this study describes the agrobacterium tumefaciens-mediated transformation of f. proliferatum as a tool for the disruptio ... | 2016 | 27323127 |
| metabolic changes in euphorbia palusrtis latex after fungal infection. | the variations of metabolic profile of the latex of wild-growing euphorbia palustris was carried out using multivariate analysis of (1)h nmr spectral data. one population was infected with fungi fusarium sporotrichioides, fusarium proliferatum and alternaria alternata, while the other consisted of healthy plant species. the non-polar metabolites of latex extracts such as benzoyl ingenol-laurate, amyrin decadienoate esters, cis-1,4-polyisoprene, and 24-methylenecycloartanol were identified using ... | 2016 | 27553715 |
| studies on organic and in-organic biostimulants in bioremediation of diesel-contaminated arable soil. | in this study, use of inorganic fertilizer (n.p.k) was compared with organic manure (compost) in the bioremediation of diesel-polluted agricultural soil over a two-month period. renewal by enhanced natural attenuation was used as control. the results revealed that total petroleum hydrocarbon removal from polluted soil was 71.40 ± 5.60% and 93.31 ± 3.60% for n.p.k and compost amended options, respectively. the control (natural attenuation) had 57.90 ± 3.98% of total petroleum hydrocarbon removed. ... | 2016 | 27494315 |
| disseminated fusariosis by fusarium proliferatum in a patient with aplastic anaemia receiving primary posaconazole prophylaxis - case report and review of the literature. | disseminated fusariosis is a life-threatening, invasive, opportunistic infection in immunocompromised patients, especially those with haematological malignancies. the prognosis is poor because these fungi are resistant to many of the available antifungal agents. we present a case of disseminated fusariosis caused by fusarium proliferatum in a patient with severe aplastic anaemia complicated by a secondary infection of aspergillus flavus, with a fatal outcome. we also review the documented fusari ... | 2016 | 26661324 |
| maize peroxidase px5 has a highly conserved sequence in inbreds resistant to mycotoxin producing fungi which enhances fungal and insect resistance. | mycotoxin presence in maize causes health and economic issues for humans and animals. although many studies have investigated expression differences of genes putatively governing resistance to producing fungi, few have confirmed a resistance role, or examined putative resistance gene structure in more than a couple of inbreds. the pericarp expression of maize px5 has previously been associated with resistance to aspergillus flavus growth and insects in a set of inbreds. genes from 14 different i ... | 2016 | 26659597 |
| defense responses to mycotoxin-producing fungi fusarium proliferatum, f. subglutinans, and aspergillus flavus in kernels of susceptible and resistant maize genotypes. | developing kernels of resistant and susceptible maize genotypes were inoculated with fusarium proliferatum, f. subglutinans, and aspergillus flavus. selected defense systems were investigated using real-time reverse transcription-polymerase chain reaction to monitor the expression of pathogenesis-related (pr) genes (pr1, pr5, prm3, prm6) and genes protective from oxidative stress (peroxidase, catalase, superoxide dismutase and ascorbate peroxidase) at 72 h postinoculation. the study was also ext ... | 2015 | 26024441 |
| fungi in housefly (musca domestica l.) as a disease risk indicator-a case study in south africa. | houseflies are the commonest insects which have increasingly overcrowded human dwellings, particularly in rural areas and constitute a health hazard. in the environment they move back and forth by feeding and breeding on food commodities and filth. this may lead to the spread of diseases and also mycotoxin-producing fungi. thus frequent exposure to the activity of such houseflies will have an impact on the welfare of humans. the study investigated the natural occurrence of fungal contamination i ... | 2014 | 25193135 |
| isolation, identification and evaluation of antimicrobial activity of streptomyces flavogriseus, strain actk2 from soil sample of kodagu, karnataka state (india). | the search for novel antibiotics continues to be of immense importance in research programs around the world for pharmaceutical, industrial and agricultural applications. filamentous soil bacteria, belonging to the streptomyces genus, are widely used as an important biological tool for their ability to produce a wide range of novel secondary metabolites, such as "antibiotics". | 2015 | 25825640 |
| phytotoxic and antibacterial metabolites from fusarium proliferatum zs07 isolated from the gut of long-horned grasshoppers. | in the proceeding of screening new bioactive natural products, the ethyl acetate extract of the fermentation broth of fusarium proliferatum zs07, a fungus residing in the gut of long-horned grasshoppers (tettigonia chinensis), was found possessing selective phytotoxic activity against the radicle growth of amaranthus retroflexus l. bioactivity-guided fractionation lead to the isolation of six fungal metabolites 1-6, including a new polyketide derivate o-methylated sma93 (2) and five known compou ... | 2014 | 25146301 |
| cloning and characterization of the gene cluster required for beauvericin biosynthesis in fusarium proliferatum. | beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activities. we isolated and characterized the fpbeas gene cluster, devoted to beauvericin biosynthesis, from the filamentous fungus fusarium proliferatum lf061. targeted inactivation of the f. proliferatum genomic copy of fpbeas abolished the production of beauvericin. comparative sequence analysis of the fpbeas showed 74% similarity with the bbbeas that synthesizes the cyclic ... | 2013 | 23832252 |
| cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer fusarium proliferatum lf061. | the ketoisovalerate reductase (ec 1.2.7.7 ) is required for the formation of beauvericin via the nonribosomal peptide synthetase biosynthetic pathway. it catalyzes the nadph-specific reduction of ketoisovaleric acid to hydroxyisovalerate. however, little is known about the bioinformatics' data about the 2-kiv reductase in fusarium. to date, heterologous production of the gene kivrfp from fusarium has not been achieved. | 2012 | 22916830 |
| the transaldolase, a novel allergen of fusarium proliferatum, demonstrates ige cross-reactivity with its human analogue. | fusarium species are among airborne fungi and recognized as causative agents of human atopic disorders. however, fusarium allergens have not been well characterized and the lack of information limits clinical diagnosis and treatment of fungal allergy. the purpose of this study is to identify and characterize important allergens of f. proliferatum. ige-reacting f. proliferatum components were identified by immunoblot using serum samples from patients of respiratory atopic diseases. characterizati ... | 2014 | 25075521 |
| diversity of endophytic fungi and screening of fungal paclitaxel producer from anglojap yew, taxus x media. | endophytic fungi represent underexplored resource of novel lead compounds and have a capacity to produce diverse class of plant secondary metabolites. here we investigated endophytic fungi diversity and screening of paclitaxel-producing fungi from taxus x media. | 2013 | 23537181 |
| endophytic fungi isolated from oil-seed crop jatropha curcas produces oil and exhibit antifungal activity. | jatropha curcas l., a perennial plant grown in tropics and subtropics is popularly known for its potential as biofuel. the plant is reported to survive under varying environmental conditions having tolerance to stress and an ability to manage pest and diseases. the plant was explored for its endophytic fungi for use in crop protection. endophytic fungi were isolated from leaf of jatropha curcas, collected from new delhi, india. four isolates were identified as colletotrichum truncatum, and other ... | 2013 | 23409154 |
| antimicrobial coumarins from the oyster culinary-medicinal mushroom, pleurotus ostreatus (agaricomycetes), from kenya. | pleurotus ostreatus has been widely used as food because of its nutritional and medicinal properties. these have been attributed to the presence of macronutrients, minerals, vitamins, and amino acids, among other secondary metabolites. there are, however, few reports on the antimicrobial activities of different classes of purified compounds from p. ostreatus. this led to the current study, the objective of which was to chemically characterize the antibiotic activities of p. ostreatus against sel ... | 2016 | 27910758 |
| cloning and functional characterization of nitrilase from fusarium proliferatum auf-2 for detoxification of nitriles. | a fungal nitrilase gene from fusarium proliferatum auf-2 was cloned through reverse transcription-pcr. the open reading frame consisted of 903 bp and potentially encoded a protein of 301 amino acid residues with a theoretical molecular mass of 33.0 kda. the encoding gene was expressed in escherichia coli strain bl21 and the recombinant protein with his6-tag was purified to electrophoretic homogeneity. the purified enzyme exhibited optimal activity in the range of 35-40 °c and ph 8.0. edta, mg(2+ ... | 2015 | 25595333 |
| cloning, bioinformatics and the enzyme activity analyses of a phenylalanine ammonia-lyase gene involved in dragon's blood biosynthesis in dracaena cambodiana. | phenylalanine ammonia-lyase (pal) is the key enzyme of the phenylpropanoid pathway, playing an important role in plant development and defence. we cloned a partial cdna of pal gene, dcpal1, from dracaena cambodiana seedlings using rt-pcr with degenerate primers that were designed based on a multiple sequence alignment of known pal genes from other plant species. dcpal1 shows highly homologous to other known pal genes registered in genbank, being closest to that of musa acuminata. dcpal1 has a re ... | 2013 | 23108992 |
| wildly growing asparagus (asparagus officinalis l.) hosts pathogenic fusarium species and accumulates their mycotoxins. | asparagus officinalis l. is an important crop in many european countries, likely infected by a number of fusarium species. most of them produce mycotoxins in plant tissues, thus affecting the physiology of the host plant. however, there is lack of information on fusarium communities in wild asparagus, where they would definitely have considerable environmental significance. therefore, the main scientific aim of this study was to identify the fusarium species and quantify their typical mycotoxins ... | 2016 | 26687343 |
| morphological and molecular characterization of fusarium spp pathogenic to pecan tree in brazil. | the occurrence of fusarium spp associated with pecan tree (carya illinoinensis) diseases in brazil has been observed in recent laboratory analyses in rio grande do sul state. thus, in this study, we i) obtained fusarium isolates from plants with disease symptoms; ii) tested the pathogenicity of these fusarium isolates to pecan; iii) characterized and grouped fusarium isolates that were pathogenic to the pecan tree based on morphological characteristics; iv) identified fusarium spp to the species ... | 2014 | 25501150 |
| synergysm of voriconazole or itraconazole with other antifungal agents against species of fusarium. | infections caused by fusarium are difficult to treat because these fungi show in vitro and in vivo resistance to practically all the antifungal agents available, which explains the high mortality rates. an attempt to overcome fungal resistance is the combination of antifungal agents, especially those with different mechanisms of action. | 2016 | 23402831 |
| [fusarium species associated with basal rot of garlic in north central mexico and its pathogenicity]. | garlic in mexico is one of the most profitable vegetable crops, grown in almost 5,451ha; out of which more than 83% are located in zacatecas, guanajuato, sonora, puebla, baja california and aguascalientes. blossom-end rot caused by fusarium spp is widely distributed worldwide and has been a limiting factor in onion and garlic production regions, not only in mexico but also in other countries. the presence of fusarium oxysporum has been reported in guanajuato and aguascalientes. fusarium culmorum ... | 2017 | 27590608 |
| essential oil composition, phytotoxic and antifungal activities of ruta chalepensis l. leaves from high atlas mountains (morocco). | this study aimed at the determination of chemical composition of essential oil obtained by hydrodistillation, and to evaluate their phytotoxic and antifungal activities. leaves of ruta chalepensis l. were collected from the region of tensift al haouz (high atlas mountains) marrakech, morocco. the essential oil (oil yield is 0.56%) was analysed by gc-fid and gc/ms. twenty-two compounds were identified and accounted for 92.4% of the total oil composition. the major components were undecan-2-one (4 ... | 2014 | 25109925 |
| identification of different fusarium spp. in allium spp. in germany. | in 2013 allium cepa bulbs from different fields in northern and southern germany, seeds and sets from onion breeders were analysed for infestation with fusarium species. the same investigation was done in 2014 with different edible allium spp. from local markets. different fusarium spp. were isolated and identified by morphological characterisation. 24 different fusarium spp. were identified. the diversity of fusarium spp. and the intensity of infestation was higher on edible bulbs compared to t ... | 2015 | 27141742 |
| molecular characterization of pathogenic fusarium species in cucurbit plants from kermanshah province, iran. | fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in kermanshah province, the largest area of cucurbits plantation in iran. therefore, the objectives in this study were to isolate and identify disease-causing fusarium spp. from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships. a total of 100 fusarium isolates were obtained from diseased cucurbit plants collected from fields ... | 2011 | 23961146 |
| in vitro combinations of natamycin with voriconazole, itraconazole and micafungin against clinical fusarium strains causing keratitis. | fusarium species cause a broad spectrum of infections, from superficial to disseminated disease. because fusarium species are intrinsically resistant to most antifungal drugs, new approaches are needed. the aim of the present study was to evaluate the in vitro combination of natamycin with currently used antifungal drugs. | 2016 | 26702918 |
| fusariotoxins in asparagus - their biosynthesis and migration. | asparagus is often infected by fungi of the fusarium genus, a causal agent of crown and root rot, which decreases the quantity and quality of spears. fusarium oxysporum and fusarium proliferatum are the most severe asparagus pathogens, well known as mycotoxin producers, mainly fumonisins and moniliformin. the present study was undertaken to estimate fumonisin b1, moniliformin and ergosterol concentrations in asparagus tissue. moreover, the possibility of toxin transport to the edible asparagus p ... | 2013 | 23756238 |
| bromelain, a cysteine protease from pineapple (ananas comosus) stem, is an inhibitor of fungal plant pathogens. | this study aimed to evaluate the effect of bromelain, a cysteine protease isolated from pineapple (ananas comosus), on growth of several agronomically important fungal pathogens. | 2012 | 22537505 |
| endophytic fungi from pigeon pea [cajanus cajan (l.) millsp.] produce antioxidant cajaninstilbene acid. | in this study, novel endophytic fungi producing cajaninstilbene acid (csa) from pigeon pea [ cajanus cajan (l.) millsp.] were investigated and screened. csa has prominent pharmacological activities. a total of 110 endophytic fungi isolates were grouped into 8 genera on the basis of morphological characteristics, and csa-producing fungi were screened by liquid chromatography-tandem mass spectrometry (lc-ms/ms). according to its-rdna sequences analysis, the csa-producing fungi were identified as f ... | 2012 | 22494407 |
| dectin-1-mediated pathway contributes to fusarium proliferatum-induced cxcl-8 release from human respiratory epithelial cells. | fusarium species are causative agents of human respiratory disorders and are distributed widely in our environment. little is known of their interaction with human respiratory epithelial cells, which may contribute to allergic airway responses. in this study, we report on the release of c-x-c motif chemokine ligand 8 (cxcl-8) from human bronchial epithelial beas-2b cells upon stimulation with fusarium proliferatum extracts. f. proliferatum-induced cytokine release from beas-2b cells was determin ... | 2017 | 28335387 |
| synthesis of a new allelopathic agent from the biotransformation of ent-15α-hydroxy-16-kauren-19-oic acid with fusarium proliferatum. | the use of kaurane diterpenes as substrates in fungal biotransformation to achieve bioactive compounds has been widely reported. in this work, the natural product kaurenoic acid, a diterpene widely distributed in the plant kingdom, was chemically converted into ent-15α-hydroxy-kaur-16-en-19-oic acid (1). substrate 1 was subjected to biotransformation by the fungus fusarium proliferatum, furnishing a new derivative, ent-2α,15α-dihydroxy-kaur-16-en-19-oic acid (2). the structure of metabolite 2 wa ... | 2017 | 28278633 |
| antifungal efficacy of plant essential oils against stored grain fungi of fusarium spp. | the control potential of seven plant essential oils was evaluated against fusarium proliferatum (matsushima) nirenberg and fusarium verticillioides sheldon. the fungicidal activity was assessed through microtiter plate assay to determine the minimum inhibitory and fungicidal concentration of essential oils. the essential oil of mentha arvensis was adjudged as best for inhibiting the fungal growth, while oil of thymus vulgaris and anethum graveolens showed high efficacy in terms of fungicidal act ... | 2016 | 28017987 |
| characterization of a new α-l-fucosidase isolated from fusarium proliferatum le1 that is regioselective to α-(1 → 4)-l-fucosidic linkage in the hydrolysis of α-l-fucobiosides. | here, we report the biochemical characterization of a novel α-l-fucosidase with broad substrate specificity (fpfuca) isolated from the mycelial fungus fusarium proliferatum le1. highly purified α-l-fucosidase was obtained from several chromatographic steps after growth in the presence of l-fucose. the purified α-l-fucosidase appeared to be a monomeric protein of 67 ± 1 kda that was able to hydrolyze the synthetic substrate p-nitrophenyl α-l-fucopyranoside (pnpfuc), with km = 1.1 ± 0.1 mm and kca ... | 2017 | 27984201 |
| targeting fumonisin biosynthetic genes. | the fungus fusarium is an agricultural problem because it can cause disease on most crop plants and can contaminate crops with mycotoxins. there is considerable variation in the presence/absence and genomic location of gene clusters responsible for synthesis of mycotoxins and other secondary metabolites among species of fusarium. here, we describe a quantitative real-time pcr (qpcr) method for distinguishing between and estimating the biomass of two closely related species, f. proliferatum and f ... | 2017 | 27924540 |