Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| light affects fumonisin production in strains of fusarium fujikuroi, fusarium proliferatum, and fusarium verticillioides isolated from rice. | three fusarium species associated with bakanae disease of rice (fusarium fujikuroi, fusarium proliferatum, and fusarium verticillioides) were investigated for their ability to produce fumonisins (fb1 and fb2) under different light conditions, and for pathogenicity. compared to darkness, the conditions that highly stimulated fumonisin production were yellow and green light in f. verticillioides strains; white and blue light, and light/dark alternation in f. fujikuroi and f. proliferatum strains. ... | 2013 | 24055868 |
| [screening and condition optimization of a strain for efficiently biotransformation of saponins in dioscorea zingiberensis into diosgenin]. | diosgenin is an important raw material in steroid hormone and widely used in pharmaceutical industry. the traditional method for diosgenin production is acidolysis, which causes serious pollution. in order to obtain a cleaner and more efficient approach of diosgenin production, a strain of gibberella intermedia wx12 (the sexual stage of fusarium proliferatum) was screened from the strains deposited in our laboratory. this strain converted saponins in dioscorea zingiberensis c.h. wright (dzw) int ... | 2013 | 24063243 |
| evaluation of ability of ferulic acid to control growth and fumonisin production of fusarium verticillioides and fusarium proliferatum on maize based media. | the aim of this study was to evaluate the efficacy of ferulic acid (1, 10, 20 and 25 mm) at different water activity (aw) values (0.99, 0.98, 0.96 and 0.93) at 25 °c on growth and fumonisin production by fusarium verticillioides and fusarium proliferatum on maize based media. for both fusarium species, the lag phase significantly decreased (p ≤ 0.001), and the growth rates increased (p ≤ 0.001) at the lowest ferulic acid concentration used (1mm), regardless of the aw. however, high doses of feru ... | 2013 | 24140805 |
| an endophytic sanguinarine-producing fungus from macleaya cordata, fusarium proliferatum blh51. | fermentation processes using sanguinarine-producing fungi other than macleaya cordata may be an alternative way to produce sanguinarine (sa), which is a quaternary benzo[c]phenanthridine alkaloid possessing antibacterial, anthelmintic, and anti-inflammatory properties. in this study, a sa-producing endophytic fungus strain blh51 was isolated from the leaves of m. cordata grown in the dabie mountain, china. strain blh51 produced sa when grown in potato dextrose liquid medium. the amount of sa pro ... | 2014 | 24166154 |
| granulomatous pododermatitis in the digits caused by fusarium proliferatum in a cat. | to the best of our knowledge, we present here the first report of a case involving granulomatous pododermatitis caused by fusarium proliferatum in a 10-year-old female cat. a cutaneous mass developed on the foot-pad of the right hind leg. nodular granulomatous dermatitis with numerous macrophages and multinucleated giant cells containing cytoplasmic fungal structures were revealed on histological examination. periodic acid-schiff reaction and fungi-fluor staining clearly revealed irregular, sept ... | 2014 | 24200892 |
| metabolism and resistance of fusarium spp. to the manzamine alkaloids via a putative retro pictet-spengler reaction and utility of the rational design of antimalarial and antifungal agents. | as a part of our continuing investigation of the manzamine alkaloids we studied the in vitro activity of the β-carboline containing manzamine alkaloids against fusarium solani, fusarium oxysporium, and fusarium proliferatum by employing several bioassay techniques including one-dimensional direct bioautography, dilution, and plate susceptibility, and microtiter broth assays. in addition, we also studied the metabolism of the manzamine alkaloids by fusarium spp. in order to facilitate the redesig ... | 2014 | 24553735 |
| natural occurrence of toxigenic fusarium proliferatum on paddy (oryza sativa l.) in karnataka, india. | contamination of paddy seeds (rice with husk) by fusarium species can cause spoilage and subsequent production of mycotoxins, especially fumonisins that affect human and animal health. a mycological study was conducted to evaluate the natural occurrence of fumonisin b1 produced by fusarium proliferatum on paddy grown in different geographic regions of karnataka (india). a total of 65 isolates of f. proliferatum from paddy samples were analysed by polymerase chain reaction (pcr). one set of prime ... | 2010 | 24575185 |
| mating populations of fusarium section liseola from rice, sugarcane and maize. | mating studies were conducted on 78 isolates of fusarium species section liseola from rice, sugarcane and maize. from the crosses with tester strains of gibberella fujikuroi species complex, 64.1% (50 out of 78 isolates) were cross-fertile with tester strains of mating populations a to e. the results of the mating studies showed that of the 50 isolates, 19 belonged to mating population a (gibberella moniliformis), 18 to mating population b (gibberella sacchari), 4 to mating population e (gibbere ... | 2011 | 24575220 |
| interactions between a sap beetle, sabal palm, scale insect, filamentous fungi and yeast, with discovery of potential antifungal compounds. | the multi-trophic relationship between insects, yeast, and filamentous fungi is reported on sabal palm (sabal palmetto (walter) lodd. ex schult. & schult. f.). gut content analyses and observations of adult and larval feeding of the sap beetle brachypeplus glaber leconte indicate that niche partitioning of fungal food substrata occurs between adults and larvae. this is the first report of specific mycophagous niche partitioning among beetle life stages based on gut content analyses. fungi isolat ... | 2014 | 24586669 |
| [breeding of high 3beta,7alpha,15alpha-trihydroxy-5-androsten-17-one transforming strains and their conversion process optimization]. | in order to improve transformation efficiency of dehydroepiandrosterone (dhea) into 3beta,7alpha,15alpha-trihydroxy-5-androsten-17-one (7alpha,15alpha-dioh-dhea) by gibberella intermedia ca3-1, we investigated the strains breeding and their conversion process optimization. g. intermedia ca3-1 strains were treated with 0.12 mg/ml 1-methyl-3-nitro-1-nitroso-guanidin (ntg) for 30 min and chosen by 350 micromol/l minimum inhibitory concentration ketoconazole resistance marker. the high production st ... | 2013 | 24701835 |
| two-dimensional environmental profiles of growth and fumonisin production by fusarium proliferatum on a wheat-based substrate. | the effect of water activity (aw; 0.995, 0.99, 0.98, 0.96, 0.94, 0.92, and 0.90), temperature (15, 25, and 30°c), incubation time (7, 14, 21 and 28days), and their interactions on mycelial growth and fumonisin production on wheat-based medium by three fusarium proliferatum strains isolated from wheat in argentina was evaluated. maximum growth rates were obtained at the highest aw (0.995) and 30°c, with growth decreasing as the aw of the medium was reduced. maximum amounts of total fumonisins (fb ... | 2014 | 24854387 |
| combined effect of chitosan and water activity on growth and fumonisin production by fusarium verticillioides and fusarium proliferatum on maize-based media. | the objectives of the present study were to determine the in vitro efficacy of chitosan (0.5, 1.0, 2.0 and 3.0mg/ml) under different water availabilities (0.995, 0.99, 0.98, 0.96 and 0.93) at 25°c on lag phase, growth rate and fumonisin production by isolates of fusarium verticillioides and fusarium proliferatum. the presence of chitosan affected growth and fumonisin production, and this effect was dependent on the dose and aw treatment used. the presence of chitosan increased the lag phase, and ... | 2014 | 24929683 |
| improving the catalytic potential and substrate tolerance of gibberella intermedia nitrilase by whole-cell immobilization. | comparative studies of immobilized and free cells of gibberella intermedia ca3-1 in bioconversion of 3-cyanopyridine to nicotinic acid were performed. entrapping method was chosen based on the advantages in enzymatic activity recovery, mechanical strength and preparation procedure. four entrapment matrices were investigated and sodium alginate was screened to be the most suitable material. maximal nitrilase activity of alginate immobilized cells was obtained under conditions of 2 % alginate, 0.6 ... | 2015 | 25030338 |
| the transaldolase, a novel allergen of fusarium proliferatum, demonstrates ige cross-reactivity with its human analogue. | fusarium species are among airborne fungi and recognized as causative agents of human atopic disorders. however, fusarium allergens have not been well characterized and the lack of information limits clinical diagnosis and treatment of fungal allergy. the purpose of this study is to identify and characterize important allergens of f. proliferatum. ige-reacting f. proliferatum components were identified by immunoblot using serum samples from patients of respiratory atopic diseases. characterizati ... | 2014 | 25075521 |
| inhibition of the phytopathogenic fungus fusarium proliferatum by volatile compounds produced by pseudomonas. | the fusarium head blight of grain cereals is a significant disease worldwide. in argentina, high levels of contamination with fusarium proliferatum have been found in crops. many strains of the pseudomonas genus antagonize the growth of fungi by different mechanisms, such as the production of antibiotics, siderophores, volatiles, and extracellular enzymes. in this work, we have designed a new system for studying the growth inhibition of f. proliferatum-namely by volatile compounds produced by ps ... | 2014 | 25085617 |
| essential oil composition, phytotoxic and antifungal activities of ruta chalepensis l. leaves from high atlas mountains (morocco). | this study aimed at the determination of chemical composition of essential oil obtained by hydrodistillation, and to evaluate their phytotoxic and antifungal activities. leaves of ruta chalepensis l. were collected from the region of tensift al haouz (high atlas mountains) marrakech, morocco. the essential oil (oil yield is 0.56%) was analysed by gc-fid and gc/ms. twenty-two compounds were identified and accounted for 92.4% of the total oil composition. the major components were undecan-2-one (4 ... | 2014 | 25109925 |
| enhanced biotransformation of dehydroepiandrosterone to 3β,7α,15α-trihydroxy-5-androsten-17-one with gibberella intermedia ca3-1 by natural oils addition. | dihydroxylation of dehydroepiandrosterone (dhea) is an essential step in the synthesis of many important pharmaceutical intermediates. however, the solution to the problem of low biohydroxylation conversion in the biotransformation of dhea has yet to be found. the effects of natural oils on the course of dihydroxylation of dhea to 3β,7α,15α-trihydroxy-5-androsten-17-one (7α,15α-dioh-dhea) were studied. with rapeseed oil (2 %, v/v) addition, the bioconversion efficiency was improved, and the 7α,1 ... | 2014 | 25145444 |
| phytotoxic and antibacterial metabolites from fusarium proliferatum zs07 isolated from the gut of long-horned grasshoppers. | in the proceeding of screening new bioactive natural products, the ethyl acetate extract of the fermentation broth of fusarium proliferatum zs07, a fungus residing in the gut of long-horned grasshoppers (tettigonia chinensis), was found possessing selective phytotoxic activity against the radicle growth of amaranthus retroflexus l. bioactivity-guided fractionation lead to the isolation of six fungal metabolites 1-6, including a new polyketide derivate o-methylated sma93 (2) and five known compou ... | 2014 | 25146301 |
| fungi in housefly (musca domestica l.) as a disease risk indicator-a case study in south africa. | houseflies are the commonest insects which have increasingly overcrowded human dwellings, particularly in rural areas and constitute a health hazard. in the environment they move back and forth by feeding and breeding on food commodities and filth. this may lead to the spread of diseases and also mycotoxin-producing fungi. thus frequent exposure to the activity of such houseflies will have an impact on the welfare of humans. the study investigated the natural occurrence of fungal contamination i ... | 2014 | 25193135 |
| the efficient production of 3β,7α,15α-trihydroxy-5-androsten-17-one from dehydroepiandrosterone by gibberella intermedia. | the biotransformation of dehydroepiandrosterone (dhea) by gibberella intermedia was investigated. the formation of the main products was monitored by high-performance liquid chromatography (hplc). products were purified and identified as 3β,7α-dihydroxy-5-androsten-17-one (7α-oh-dhea) and 3β,7α,15α-trihydroxy-5-androsten-17-one (7α,15α-dioh-dhea) using mass spectroscopy (ms) and nuclear magnetic resonance (nmr) analyses. 7α,15α-dioh-dhea is a key intermediate for the synthesis of pharmacological ... | 2014 | 25245679 |
| fungaemia caused by fusarium proliferatum in a patient without definite immunodeficiency. | recent literature has shown the growing importance of opportunistic fungal infections due to fusarium spp. however, disseminated fusariosis remains rare in patients without neutropenia. we report a case of fungaemia in a 78-year-old french woman without definite immunodeficiency. fusarium proliferatum grew from both central and peripheral blood cultures. fever was the only clinical sign of the infection. an appropriate antifungal therapy with voriconazole led to the recovery of the patient. an e ... | 2015 | 25253233 |
| the novel strain fusarium proliferatum le1 (rcam02409) produces α-l-fucosidase and arylsulfatase during the growth on fucoidan. | enzymes capable of modifying the sulfated polymeric molecule of fucoidan are mainly produced by different groups of marine organisms: invertebrates, bacteria, and also some fungi. we have discovered and identified a new strain of filamentous fungus fusarium proliferatum le1 (deposition number in russian collection of agricultural microorganisms is rcam02409), which is a potential producer of fucoidan-degrading enzymes. the strain le1 (rcam02409) was identified on the basis of morphological chara ... | 2015 | 25346501 |
| host extract modulates metabolism and fumonisin biosynthesis by the plant-pathogenic fungus fusarium proliferatum. | fusarium proliferatum is a common pathogen able to infect a broad range of agriculturally important crops. recently, some evidence for genetic variance among the species genotypes in relation to their plant origin has been reported. mycotoxin contamination of plant tissues is the most important threat caused by f. proliferatum and fumonisins b (fbs) are the principal mycotoxins synthesized. the toxigenic potential of the pathogen genotypes is variable and also the reaction of different host plan ... | 2015 | 25462926 |
| morphological and molecular characterization of fusarium spp pathogenic to pecan tree in brazil. | the occurrence of fusarium spp associated with pecan tree (carya illinoinensis) diseases in brazil has been observed in recent laboratory analyses in rio grande do sul state. thus, in this study, we i) obtained fusarium isolates from plants with disease symptoms; ii) tested the pathogenicity of these fusarium isolates to pecan; iii) characterized and grouped fusarium isolates that were pathogenic to the pecan tree based on morphological characteristics; iv) identified fusarium spp to the species ... | 2014 | 25501150 |
| cloning and functional characterization of nitrilase from fusarium proliferatum auf-2 for detoxification of nitriles. | a fungal nitrilase gene from fusarium proliferatum auf-2 was cloned through reverse transcription-pcr. the open reading frame consisted of 903 bp and potentially encoded a protein of 301 amino acid residues with a theoretical molecular mass of 33.0 kda. the encoding gene was expressed in escherichia coli strain bl21 and the recombinant protein with his6-tag was purified to electrophoretic homogeneity. the purified enzyme exhibited optimal activity in the range of 35-40 °c and ph 8.0. edta, mg(2+ ... | 2015 | 25595333 |
| genetic variability and fumonisin production by fusarium proliferatum isolated from durum wheat grains in argentina. | fusarium proliferatum is a member of the fusarium fujikuroi species complex (ffsc) involved in the maize ear rot together with fusarium verticillioides, which is a very closely related species. recently, different studies have detected natural fumonisin contamination in wheat kernels and most of them have shown that the main species isolated was f. proliferatum. fusarium strains obtained from freshly harvested durum wheat samples (2008 to 2011 harvest seasons) from argentina were characterized t ... | 2015 | 25732000 |
| carbon and nitrogen limitation increase chitosan antifungal activity in neurospora crassa and fungal human pathogens. | chitosan permeabilizes plasma membrane and kills sensitive filamentous fungi and yeast. membrane fluidity and cell energy determine chitosan sensitivity in fungi. a five-fold reduction of both glucose (main carbon (c) source) and nitrogen (n) increased 2-fold neurospora crassa sensitivity to chitosan. we linked this increase with production of intracellular reactive oxygen species (ros) and plasma membrane permeabilization. releasing n. crassa from nutrient limitation reduced chitosan antifungal ... | 2015 | 25749367 |
| isolation, identification and evaluation of antimicrobial activity of streptomyces flavogriseus, strain actk2 from soil sample of kodagu, karnataka state (india). | the search for novel antibiotics continues to be of immense importance in research programs around the world for pharmaceutical, industrial and agricultural applications. filamentous soil bacteria, belonging to the streptomyces genus, are widely used as an important biological tool for their ability to produce a wide range of novel secondary metabolites, such as "antibiotics". | 2015 | 25825640 |
| concatemerization increases the inhibitory activity of short, cell-penetrating, cationic and tryptophan-rich antifungal peptides. | there are short cationic and tryptophan-rich antifungal peptides such as the hexapeptide paf26 (rkkwfw) that have selective toxicity and cell penetration properties against fungal cells. this study demonstrates that concatemeric peptides with tandem repeats of the heptapeptide paf54 (which is an elongated paf26 sequence) show increased fungistatic and bacteriostatic activities while maintaining the absence of hemolytic activity of the monomer. the increase in antimicrobial activity of the double ... | 2015 | 25846331 |
| [isolation of endophytic fungi from macleaya cordata and screening of sanguinarine-producing strains]. | endophytic fungi were isolated from macleaya cordata growing in dabie mountain by agar-block method, and then the endophytic fungi were grouped into different types based on their morphological characteristics, and thin layer chromatography (tlc) and high performance liquid chromatography (hplc) were employed to determine whether the metabolic substances contained sanguinarine or not, and then preliminarily identified by morphological method. the results showed that the leaves hosted the largest ... | 2014 | 25850254 |
| fungal rhino sinusitisin in tehran, iran. | fungal rhino sinusitis (frs) is an important infection of para nasal sinuses, which encompasses two main categories; invasive and noninvasive forms according to histopathological findings. aspergillus spp are the most common species isolated from noninvasive form, while mucorales are more frequently isolates from acute infections. | 2015 | 25905081 |
| fumonisin b1 induces autophagic cell death via activation of ern1-mapk8/9/10 pathway in monkey kidney marc-145 cells. | mycotoxins are secondary fungal metabolites that are capable of inducing a variety of toxic effects in animals and humans resulting from the consumption of the contaminated food. understanding the mechanisms of the toxicities behind these mycotoxins is required to develop mechanism-based approach to counteract their toxic potential. fumonisin b1 (fb1) is the most prevalent member of fumonisins that are a group of mycotoxins produced primarily by fusarium verticillioides and fusarium proliferatum ... | 2016 | 25925693 |
| defense responses to mycotoxin-producing fungi fusarium proliferatum, f. subglutinans, and aspergillus flavus in kernels of susceptible and resistant maize genotypes. | developing kernels of resistant and susceptible maize genotypes were inoculated with fusarium proliferatum, f. subglutinans, and aspergillus flavus. selected defense systems were investigated using real-time reverse transcription-polymerase chain reaction to monitor the expression of pathogenesis-related (pr) genes (pr1, pr5, prm3, prm6) and genes protective from oxidative stress (peroxidase, catalase, superoxide dismutase and ascorbate peroxidase) at 72 h postinoculation. the study was also ext ... | 2015 | 26024441 |
| a case of fungus ball-type pansinusitis due to fusarium proliferatum. | incidence of fungal sinusitis due to the genus fusarium has increased during the last two decades. we report a case of fungus ball sinusitis with multiple sinuses involvement in an iranian 21-year-old woman. the patient was diagnosed as having a fungus ball-type sinusitis in computed tomography scan. the sinus biopsy revealed fungal structures on histopathological and direct microscopic examinations and a fusarium species arose in culture. partial sequencing of the translation elongation factor ... | 2015 | 26025662 |
| fungal endophytes of vanilla planifolia across réunion island: isolation, distribution and biotransformation. | the objective of the work was to characterize fungal endophytes from aerial parts of vanilla planifolia. also, to establish their biotransformation abilities of flavor-related metabolites. this was done in order to find a potential role of endophytes on vanilla flavors. | 2015 | 26070309 |
| bioactive compounds from the endophytic fungus fusarium proliferatum. | the crude extract of an endophytic fungus isolated from syzygium cordatum and identified as fusarium proliferatum showed 100% cytotoxicity against the brine shrimp artemia salina at 100 μg/ml. seven coloured, biologically active metabolites - including ergosta-5,7,22-trien-3β-ol, nectriafurone-8-methyl ether, 9-o-methyl fusarubin, bostrycoidin, bostrycoidin-9-methyl ether and 8-hydroxy-5,6-dimethoxy-2-methyl-3-(2-oxo-propyl)-1,4-naphthoquinone- were isolated from the extract. | 2016 | 26158312 |
| dermatitis in the fringe-toed lizard, acanthodactylus nilsoni rastegar-pouyani, 1998 (sauria: lacertidae) associated with fusarium proliferatum. | from july to october 2013, nine out of 40 acanthodactylus nilsoni collected from western iran, showed clinical signs of dermatitis in the dorsal and ventral surface of neck and fingers. therefore, the aim of this survey was to identify the fungal flora colonizing the skin of a. nilsoni using morphological and molecular studies. nine isolates of fusarium were obtained from infected lizard samples and identified as fusarium proliferatum through study of morphological characters. in the present stu ... | 2015 | 26292787 |
| urinary tract infection (uti) caused by fusarium proliferatum in an agranulocytosis patient and a review of published reports. | infections caused by fusarium species are increasing in frequency among immunocompromised hosts, but urinary tract infection (uti) due to fusarium proliferatum has not been reported in the literature so far. we describe a case of uti caused by f. proliferatum in a 47-year-old man who was diagnosed with rectal cancer and metastasis. he underwent radical resection of rectal carcinoma and local resection of hepatic metastases. after the first adjuvant chemotherapy, the patient presented the obvious ... | 2016 | 26346378 |
| effects of fusaric acid treatment on the protocorm-like bodies of dendrobium sonia-28. | dendrobium sonia-28 is a popular orchid hybrid due to its flowering recurrence and dense inflorescences. unfortunately, it is being decimated by fungal diseases, especially those caused by fusarium proliferatum. in this study, selection of f. proliferatum-tolerant protocorm-like bodies (plbs) was carried out by assessing the effects of differing concentrations of fusaric acid (fa). plbs were cultured on murashige and skoog (ms) medium supplemented with 0.05 to 0.2 millimolar (mm) concentrations ... | 2016 | 26471909 |
| development of a sensitive, competitive, indirect elisa for the detection of fumonisin b1 in corn originating from anhui province, china. | fumonisin b1 (fb1) is a secondary metabolite produced by fusarium verticillioides or fusarium proliferatum, which present in food and feed. it causes hazardous effects on human and animal health. a monoclonal antibody (mab) against fb1 was produced and a simple, reliable and sensitive, competitive, indirect enzyme-linked immunosorbent assay (ci-elisa) for detection of fb1 was developed and the experiment conditions were optimized. the coating concentration of fb1-ovalbumin (fb1-ova) was 500 ng m ... | 2016 | 26621078 |
| maize peroxidase px5 has a highly conserved sequence in inbreds resistant to mycotoxin producing fungi which enhances fungal and insect resistance. | mycotoxin presence in maize causes health and economic issues for humans and animals. although many studies have investigated expression differences of genes putatively governing resistance to producing fungi, few have confirmed a resistance role, or examined putative resistance gene structure in more than a couple of inbreds. the pericarp expression of maize px5 has previously been associated with resistance to aspergillus flavus growth and insects in a set of inbreds. genes from 14 different i ... | 2016 | 26659597 |
| disseminated fusariosis by fusarium proliferatum in a patient with aplastic anaemia receiving primary posaconazole prophylaxis - case report and review of the literature. | disseminated fusariosis is a life-threatening, invasive, opportunistic infection in immunocompromised patients, especially those with haematological malignancies. the prognosis is poor because these fungi are resistant to many of the available antifungal agents. we present a case of disseminated fusariosis caused by fusarium proliferatum in a patient with severe aplastic anaemia complicated by a secondary infection of aspergillus flavus, with a fatal outcome. we also review the documented fusari ... | 2016 | 26661324 |
| wildly growing asparagus (asparagus officinalis l.) hosts pathogenic fusarium species and accumulates their mycotoxins. | asparagus officinalis l. is an important crop in many european countries, likely infected by a number of fusarium species. most of them produce mycotoxins in plant tissues, thus affecting the physiology of the host plant. however, there is lack of information on fusarium communities in wild asparagus, where they would definitely have considerable environmental significance. therefore, the main scientific aim of this study was to identify the fusarium species and quantify their typical mycotoxins ... | 2016 | 26687343 |
| in vitro combinations of natamycin with voriconazole, itraconazole and micafungin against clinical fusarium strains causing keratitis. | fusarium species cause a broad spectrum of infections, from superficial to disseminated disease. because fusarium species are intrinsically resistant to most antifungal drugs, new approaches are needed. the aim of the present study was to evaluate the in vitro combination of natamycin with currently used antifungal drugs. | 2016 | 26702918 |
| development of simple sequence repeat (ssr) markers for discrimination among isolates of fusarium proliferatum. | the plant pathogen fusarium proliferatum has a wide host range and occurs worldwide. many isolates of the fungus produce mycotoxins in plant tissues, which, if ingested, can cause harm to animals and humans. in 2008, an outbreak of salmon blotch of onions, caused by f. proliferatum, was detected in southern israel. the source and distribution of the fungus in israel were unknown. inter-simple sequence repeats (issr) were used to identify repetitive motifs present in seven isolates of f. prolifer ... | 2016 | 27021663 |
| identification of different fusarium spp. in allium spp. in germany. | in 2013 allium cepa bulbs from different fields in northern and southern germany, seeds and sets from onion breeders were analysed for infestation with fusarium species. the same investigation was done in 2014 with different edible allium spp. from local markets. different fusarium spp. were isolated and identified by morphological characterisation. 24 different fusarium spp. were identified. the diversity of fusarium spp. and the intensity of infestation was higher on edible bulbs compared to t ... | 2015 | 27141742 |
| inhibitory mechanism of butylated hydroxyanisole against infection of fusarium proliferatum based on comparative proteomic analysis. | fusarium proliferatum as a filamentous fungal pathogen can produce mycotoxins that can contaminate postharvest fruits and thus impact risks on human health. the extracellular proteomes of f. proliferatum grown in the absence and presence of butylated hydroxyanisole (bha) were analyzed comparatively. a total of 66 significantly different expressed secreted proteins were identified by lc-esi-ms/ms analysis. the bha treatment suppressed the accumulation of some pathogenic factors such as aspartic p ... | 2016 | 27169933 |
| new cyclohexadepsipeptides from an entomogenous fungus fusarium proliferatum and their cytotoxicity and autophagy-inducing activity. | five new cyclohexadepsipeptides termed as enniatins r - v (1 - 5) and seven known cyclohexadepsipeptides (6 - 12) were isolated from the solid culture of fusarium proliferatum, a fungus isolated from the cadaver of an unidentified insect collected in tibet. their structures were elucidated by nmr and ms spectroscopic analysis. the x-ray single-crystal structure of 6 was reported for the first time. enniatins r and s represented the first enniatins incorporating with an unusual 2,3-dihydroxy-isov ... | 2016 | 27233912 |
| fusarium proliferatum strains change fumonisin biosynthesis and accumulation when exposed to host plant extracts. | fumonisin concentrations in mycelia and media were studied in liquid fusarium proliferatum cultures supplemented with host plant extracts. furthermore, the kinetics of fumonisin accumulation in media and mycelia collected before and after extract addition was analysed as well as the changes in the expression of the fum1 gene. fumonisin content in culture media increased in almost all f. proliferatum strains shortly after plant extracts were added. the asparagus extract induced the highest fb lev ... | 2016 | 27268248 |
| antifungal attributes of lactobacillus plantarum mys6 against fumonisin producing fusarium proliferatum associated with poultry feeds. | fumonisins, being common in occurrence in maize-based feeds, pose a great threat to animal and human health. the present study is aimed at determining the antifungal activity of lactobacillus plantarum mys6 against a fumonisin producing fungus, fusarium proliferatum mys9. the isolate was subjected to standard tests for determining its probiotic attributes and antifungal properties. l. plantarum mys6 thrived well at ph 3.0 and 6.0, and exhibited strong resistance up to 3% bile. the isolate showed ... | 2016 | 27285317 |
| agrobacterium-mediated transformation of fusarium proliferatum. | fusarium proliferatum is an important pathogen that is associated with plant diseases and primarily affects aerial plant parts by producing different mycotoxins, which are toxic to humans and animals. within the last decade, this fungus has also been described as one of the causes of red root rot or sudden death syndrome in soybean, which causes extensive damage to this crop. this study describes the agrobacterium tumefaciens-mediated transformation of f. proliferatum as a tool for the disruptio ... | 2016 | 27323127 |
| detection of antibiotic-resistant bacteria endowed with antimicrobial activity from a freshwater lake and their phylogenetic affiliation. | antimicrobial resistance poses a serious challenge to global public health. in this study, fifty bacterial strains were isolated from the sediments of a freshwater lake and were screened for antibiotic resistance. out of fifty isolates, thirty-three isolates showed resistance against at least two of the selected antibiotics. analysis of 16s rdna sequencing revealed that the isolates belonged to ten different genera, namely staphylococcus(n = 8), bacillus(n = 7), lysinibacillus(n = 4), achromobac ... | 2016 | 27330861 |
| vacuolar serine protease is a major allergen of fusarium proliferatum and an ige-cross reactive pan-fungal allergen. | fusarium species are among prevalent airborne fungi and causative agents of human respiratory atopic disorders. we previously identified a 36.5-kda f. proliferatum component recognized by ige antibodies in 9 (53%) of the 17 f. proliferatum-sensitized atopic serum samples. the purpose of this study is to characterize the 36.5-kda allergen of f. proliferatum. | 2016 | 27334782 |
| quantitative determination of fusarium proliferatum concentration in intact garlic cloves using near-infrared spectroscopy. | fusarium proliferatum is considered to be a pathogen of many economically important plants, including garlic. the objective of this research was to apply near-infrared spectroscopy (nirs) to rapidly determine fungal concentration in intact garlic cloves, avoiding the laborious and time-consuming procedures of traditional assays. preventive detection of infection before seeding is of great interest for farmers, because it could avoid serious losses of yield during harvesting and storage. spectra ... | 2016 | 27428978 |
| influence of butylated hydroxyanisole on the growth, hyphal morphology, and the biosynthesis of fumonisins in fusarium proliferatum. | fusarium proliferatum as a common fungus pathogen in foods can produce toxic fumonisins, which can cause animal diseases and increase risks of human cancers. on contrary, butylated hydroxyanisole (bha) as a synthetic antioxidant offers a clue for preventing growth of fungal species and inhibiting production of mycotoxins. unfortunately, information of the inhibitory mechanism of bha on fusarium species is still limited. in this study, influence of bha treatment on growth and inhibition of fumoni ... | 2016 | 27468276 |
| studies on organic and in-organic biostimulants in bioremediation of diesel-contaminated arable soil. | in this study, use of inorganic fertilizer (n.p.k) was compared with organic manure (compost) in the bioremediation of diesel-polluted agricultural soil over a two-month period. renewal by enhanced natural attenuation was used as control. the results revealed that total petroleum hydrocarbon removal from polluted soil was 71.40 ± 5.60% and 93.31 ± 3.60% for n.p.k and compost amended options, respectively. the control (natural attenuation) had 57.90 ± 3.98% of total petroleum hydrocarbon removed. ... | 2016 | 27494315 |
| maize leaf trichomes represent an entry point of infection for fusarium species. | fifteen day old maize seedlings were inoculated with fusarium graminearum, fusarium proliferatum, and fusarium verticillioides. more than 90 % f. proliferatum and f. verticillioides conidia and 50 % of f. graminearum formed one germ tube whereas the other 50 % of f. graminearum conidia formed two to three germ tubes. the germ tubes of f. graminearum conidia were longer than those of f. proliferatum and f. verticillioides. the three species of fusarium infected bi-cellular trichomes by adhering a ... | 2016 | 27521623 |
| species-specific optical genosensors for the detection of mycotoxigenic fusarium fungi in food samples. | plant-pathogenic fusarium species, fusarium verticillioides and fusarium proliferatum, are the major producers of fumonisins which are one of the most common mycotoxins found in maize. herein, we report the development of specific and sensitive genosensors for detecting these two closely related fusarium species in food samples. the sensors are based on species-specific capture and detection probes, which bind to the intergenic spacer region of rdna (igs). oligonucleotide functionalized magnetic ... | 2016 | 27543032 |
| metabolic changes in euphorbia palusrtis latex after fungal infection. | the variations of metabolic profile of the latex of wild-growing euphorbia palustris was carried out using multivariate analysis of (1)h nmr spectral data. one population was infected with fungi fusarium sporotrichioides, fusarium proliferatum and alternaria alternata, while the other consisted of healthy plant species. the non-polar metabolites of latex extracts such as benzoyl ingenol-laurate, amyrin decadienoate esters, cis-1,4-polyisoprene, and 24-methylenecycloartanol were identified using ... | 2016 | 27553715 |
| histopathological assessment of the infection of maize leaves by fusarium graminearum, f. proliferatum, and f. verticillioides. | young maize plants were inoculated on unfolded mature leaves and on folded immature leaves with fusarium graminearum, fusarium proliferatum, and fusarium verticillioides suspensions. infection and symptom development of disease on these asymptomatic mature leaves and immature leaves were then documented. subcuticular infection was found by the three fusarium species on both symptomatic and symptomless leaves. the three fusarium species penetrated the stomata of immature leaves by the formation o ... | 2016 | 27567716 |
| a novel population of fusarium fujikuroi isolated from southeastern u.s. winegrapes reveals the need to re-evaluate the species' fumonisin production. | mycotoxins pose a challenge to a safe food supply worldwide, and their threat is expected to worsen with our changing climate. the need for diligence is exemplified by the discovery of fumonisin b2 in wine, which joins ochratoxin a as a mycotoxin of concern in the grape-wine chain. to elucidate the mycotoxin risk in southeastern american wine, grape samples were collected from vineyards during harvest in 2013 and potentially mycotoxigenic fungi (fusarium and aspergillus) were isolated from the s ... | 2016 | 27589800 |
| [fusarium species associated with basal rot of garlic in north central mexico and its pathogenicity]. | garlic in mexico is one of the most profitable vegetable crops, grown in almost 5,451ha; out of which more than 83% are located in zacatecas, guanajuato, sonora, puebla, baja california and aguascalientes. blossom-end rot caused by fusarium spp is widely distributed worldwide and has been a limiting factor in onion and garlic production regions, not only in mexico but also in other countries. the presence of fusarium oxysporum has been reported in guanajuato and aguascalientes. fusarium culmorum ... | 2017 | 27590608 |
| ungual hyalohyphomycosis caused by fusarium proliferatum in an immunocompetent patient. | the patient was a 73-year-old healthy female farmer who had been treated with terbinafine for 25.5 months by a primary physician. she exhibited a discoloration and thickening of the right big toenail. she had no concomitant paronychia. direct microscopy revealed chlamydoconidia and hyphae, and periodic acid-schiff stained nail specimen showed septate hyphae. on the basis of these morphological features and gene analysis, the final diagnosis was ungual hyalohyphomycosis caused by fusarium prolife ... | 2017 | 27607919 |
| antimicrobial coumarins from the oyster culinary-medicinal mushroom, pleurotus ostreatus (agaricomycetes), from kenya. | pleurotus ostreatus has been widely used as food because of its nutritional and medicinal properties. these have been attributed to the presence of macronutrients, minerals, vitamins, and amino acids, among other secondary metabolites. there are, however, few reports on the antimicrobial activities of different classes of purified compounds from p. ostreatus. this led to the current study, the objective of which was to chemically characterize the antibiotic activities of p. ostreatus against sel ... | 2016 | 27910758 |
| targeting conserved genes in fusarium species. | fumonisins are important mycotoxins contaminating foods and feeds which are mainly produced by f. verticillioides and f. proliferatum. additionally, both are pathogens of maize and other cereals. we describe two highly sensitive, rapid, and species-specific pcr protocols which enable detection and discrimination of these closely related species in cereal flour or grain samples. the specific primer pairs of these assays were based on the intergenic spacer region of the multicopy rdna unit which h ... | 2017 | 27924535 |
| targeting fumonisin biosynthetic genes. | the fungus fusarium is an agricultural problem because it can cause disease on most crop plants and can contaminate crops with mycotoxins. there is considerable variation in the presence/absence and genomic location of gene clusters responsible for synthesis of mycotoxins and other secondary metabolites among species of fusarium. here, we describe a quantitative real-time pcr (qpcr) method for distinguishing between and estimating the biomass of two closely related species, f. proliferatum and f ... | 2017 | 27924540 |
| characterization of a new α-l-fucosidase isolated from fusarium proliferatum le1 that is regioselective to α-(1 → 4)-l-fucosidic linkage in the hydrolysis of α-l-fucobiosides. | here, we report the biochemical characterization of a novel α-l-fucosidase with broad substrate specificity (fpfuca) isolated from the mycelial fungus fusarium proliferatum le1. highly purified α-l-fucosidase was obtained from several chromatographic steps after growth in the presence of l-fucose. the purified α-l-fucosidase appeared to be a monomeric protein of 67 ± 1 kda that was able to hydrolyze the synthetic substrate p-nitrophenyl α-l-fucopyranoside (pnpfuc), with km = 1.1 ± 0.1 mm and kca ... | 2017 | 27984201 |
| antifungal efficacy of plant essential oils against stored grain fungi of fusarium spp. | the control potential of seven plant essential oils was evaluated against fusarium proliferatum (matsushima) nirenberg and fusarium verticillioides sheldon. the fungicidal activity was assessed through microtiter plate assay to determine the minimum inhibitory and fungicidal concentration of essential oils. the essential oil of mentha arvensis was adjudged as best for inhibiting the fungal growth, while oil of thymus vulgaris and anethum graveolens showed high efficacy in terms of fungicidal act ... | 2016 | 28017987 |
| synthesis of a new allelopathic agent from the biotransformation of ent-15α-hydroxy-16-kauren-19-oic acid with fusarium proliferatum. | the use of kaurane diterpenes as substrates in fungal biotransformation to achieve bioactive compounds has been widely reported. in this work, the natural product kaurenoic acid, a diterpene widely distributed in the plant kingdom, was chemically converted into ent-15α-hydroxy-kaur-16-en-19-oic acid (1). substrate 1 was subjected to biotransformation by the fungus fusarium proliferatum, furnishing a new derivative, ent-2α,15α-dihydroxy-kaur-16-en-19-oic acid (2). the structure of metabolite 2 wa ... | 2017 | 28278633 |
| dectin-1-mediated pathway contributes to fusarium proliferatum-induced cxcl-8 release from human respiratory epithelial cells. | fusarium species are causative agents of human respiratory disorders and are distributed widely in our environment. little is known of their interaction with human respiratory epithelial cells, which may contribute to allergic airway responses. in this study, we report on the release of c-x-c motif chemokine ligand 8 (cxcl-8) from human bronchial epithelial beas-2b cells upon stimulation with fusarium proliferatum extracts. f. proliferatum-induced cytokine release from beas-2b cells was determin ... | 2017 | 28335387 |
| corrigendum to "characterization of a new α-l-fucosidase isolated from fusarium proliferatum le1 that is regioselective to α-(1 → 4)-l-fucosidic linkage in the hydrolysis of α-l-fucobiosides" [biochimie 132c (2017) 54-65]. | 2017 | 28434920 | |
| abiotic conditions leading to fum gene expression and fumonisin accumulation by fusarium proliferatum strains grown on a wheat-based substrate. | fusarium proliferatum produces fumonisins b not only on maize but also on diverse crops including wheat. using a wheat-based medium, the effects of abiotic factors, temperature and water activity (aw), on growth, fumonisin biosynthesis, and expression of fum genes were compared for three f. proliferatum strains isolated from durum wheat in argentina. although all isolates showed similar profiles of growth, the fumonisin production profiles were slightly different. regarding fum gene transcriptio ... | 2017 | 28463723 |
| co-culturing of fungal strains against botrytis cinerea as a model for the induction of chemical diversity and therapeutic agents. | new fungal sms (sms) have been successfully described to be produced by means of in vitro-simulated microbial community interactions. co-culturing of fungi has proved to be an efficient way to induce cell-cell interactions that can promote the activation of cryptic pathways, frequently silent when the strains are grown in laboratory conditions. filamentous fungi represent one of the most diverse microbial groups known to produce bioactive natural products. triggering the production of novel anti ... | 2017 | 28469610 |
| the endophytic fungi of salvia miltiorrhiza bge.f. alba are a potential source of natural antioxidants. | salvia miltiorrhiza bge. f. alba is a traditional chinese herbal drug with special pharmacological effect on thromboangiitis obliterans. however, the nature source of s.miltiorrhiza bge.f.alba is now in short supply because of the over-collection of the wild plant. to better utilize this resource, the diversity and antioxidant activity of endophytic fungi isolated from s. miltiorrhiza bge. f. alba were investigated. | 2015 | 28510814 |
| proteomics analysis of fusarium proliferatum under various initial ph during fumonisin production. | fusarium proliferatum as a fungal pathogen can produce fumonisin which causes a great threat to animal and human health. proteomic approach was a useful tool for investigation into mycotoxin biosynthesis in fungal pathogens. in this study, we analyzed the fumonisin content and mycelium proteins of fusarium proliferatum cultivated under the initial ph5 and 10. fumonisin production after 10days was significantly induced in culture condition at ph10 than ph5. ninety nine significantly differently a ... | 2017 | 28522339 |
| fusarium proliferatum - causal agent of garlic bulb rot in spain: genetic variability and mycotoxin production. | fusarium proliferatum is a world-wide occurring fungal pathogen affecting several crops included garlic bulbs. in spain, this is the most frequent pathogenic fungus associated with garlic rot during storage. moreover, f. proliferatum is an important mycotoxigenic species, producing a broad range of toxins, which may pose a risk for food safety. the aim of this study is to assess the intraspecific variability of the garlic pathogen in spain implied by analyses of translation elongation factor (te ... | 2017 | 28648292 |
| heterologous expression in pichia pastoris and biochemical characterization of the unmodified sulfatase from fusarium proliferatum le1. | sulfatases are a family of enzymes (sulfuric ester hydrolases, ec 3.1.6.-) that catalyze the hydrolysis of a wide array of sulfate esters. to date, despite the discovery of many sulfatase genes and the accumulation of data on numerous sulfated molecules, the number of characterized enzymes that are key players in sulfur metabolism remains extremely limited. while mammalian sulfatases are well studied due to their involvement in a wide range of normal and pathological biological processes, lower ... | 2017 | 28651356 |
| host extracts induce changes in the proteome of plant pathogen fusarium proliferatum. | fusarium proliferatum is a polyphagous pathogenic fungus able to infect many crop plants worldwide. differences in proteins accumulated were observed when maize- and asparagus-derived f. proliferatum strains were exposed to host extracts prepared from asparagus, maize, garlic, and pineapple tissues. seventy-three unique proteins were up-regulated in extract-supplemented cultures compared to the controls. they were all identified using mass spectrometry and their putative functions were assigned. ... | 2017 | 28705396 |
| detection of fumonisins in fresh and dehydrated commercial garlic. | an epidemic fungal disease caused by fusarium proliferatum, responsible for fumonisin production (fb1, fb2, and fb3), has been reported in the main garlic-producing countries in recent years. fumonisins are a group of structurally related toxic metabolites produced by this pathogen. the aim of this work was to establish an enzyme-linked immunosorbent assay (elisa) procedure, mostly applied to cereals, that is suitable for fumonisin detection in garlic and compare these results to those obtained ... | 2017 | 28719747 |
| skin lesion in a patient after hematopoietic stem cell transplant. | we present a case of a 61-year-old caucasian woman who was hospitalized with fevers on day 176 after a matched unrelated stem cell transplant for acute myelogenous leukemia. she developed hemorrhagic bullae on the skin of her right thigh, and blood cultures and skin biopsy both confirmed fusarium proliferatum. despite antifungal therapy, her condition worsened and she died while on comfort-only measures. this article is protected by copyright. all rights reserved. | 2017 | 28741746 |
| pathological interrelations of soil-borne diseases in cucurbits caused by fusarium species and meloidogyne incognita. | pathological interrelations of two soil-borne diseases in cucurbits (watermelon, oriental melon, shintosa and cucumber) caused by fusarium isolates (fi) and the root-knot nematode (rkn), meloidogyne incognita were characterized by the fusarium disease severity index (di), rkn gall index (gi) and eggmass index (ei) in inoculation tests using fi and rkn. virulence of fi as determined by di at 4 weeks after inoculation was mostly in the higher order of fusarium proliferatum f6, f5 and fusarium oxys ... | 2017 | 28811758 |
| carbon sources influence fumonisin production in fusarium proliferatum. | fusarium proliferatum is a world-wide fungal pathogen that produces fumonisins which are harmful to animal and human health. however, environmental factors affecting fumonisin biosynthesis in f. proliferatum are not well understood. based on our preliminary results, in this study, we investigated the effect of sucrose or mannose as the sole carbon source on fumonisin b (fb) production by f. proliferatum and studied their underlying mechanisms via proteome and gene expression analysis. our result ... | 2017 | 28834107 |
| amycolatopsis acidiphila sp. nov., a moderately acidophilic species isolated from coal mine soil. | little is known on members of the genus amycolatopsis inhabiting acidic habitats. in this study, a moderately acidophilic amycolatopsis strain, designated 2-5t, was isolated from coal mine soil, and subjected to a polyphasic taxonomic characterization. analysis based on 16s rrna gene sequences indicated that the strain was most closely related to the type strain of amycolatopsis bartoniae, sharing 99.30 % similarity, while similarity to all other amycolatopsis species was less than 97 %. the dna ... | 2017 | 28857027 |