Publications

mould germination: data treatment and modelling.	the objectives of this study were i/ to examine germination data sets over a range of environmental conditions (water activity, temperature) for eight food spoilage moulds, ii/ to compare the ability of the gompertz equation and logistic function to fit the experimental plots, iii/ to simulate germination by assessing various distributions of the latent period for germination amongst a population of spores. data sets (percentage germination, p (%), versus time, t) of aspergillus carbonarius, asp ...	2007	17188772
fungi recovered from slovakian poultry feed mixtures and their toxinogenity.	to contribute towards the knowledge of microbiology of feeds, more than 100 samples of poultry feed mixtures from slovakia were mycologically investigated in terms of the overall fungal diversity and toxicological potential of isolated fungi. the study revealed that out of 22 genera recovered, penicillium was the most frequent and diverse genus, followed by aspergillus and mucor being found in 89 % (34 spp.), 69 % (11 spp.) and 50 % (4 spp.), respectively. the most frequently encountered taxa we ...	2006	17195991
contamination of pine nuts by fumonisin produced by strains of fusarium proliferatum isolated from pinus pinea.	to test the ability of fusarium proliferatum strains isolated from pinus pinea to synthesize fumonisin b(1) (fb(1)) in pine nuts.	2007	17209817
biodiversity of fusarium species in mexico associated with ear rot in maize, and their identification using a phylogenetic approach.	fusarium proliferatum, f. subglutinans, and f. verticillioides are known causes of ear and kernel rot in maize worldwide. in mexico, only f. verticillioides and f. subglutinans, have been reported previously as causal agents of this disease. however, fusarium isolates with different morphological characteristics to the species that are known to cause this disease were obtained in the highland-valley region of this country from symptomatic and symptomless ears of native and commercial maize genot ...	2007	17216329
cloning and characterization of fpmtr1, an amino acid transporter gene of fusarium proliferatum (gibberella intermedia).	fpmtr1, an amino acid transporter gene from fusarium proliferatum was strongly expressed during conidial germination and repressed in late stationary phase. to identify the specific function of this gene, deltafpmtr1 knock-out mutants were generated by gene replacement. vegetative growth of the deltafpmtr1 mutants was normal both in liquid and on solid media, but conidial germination was delayed. the deltafpmtr1 mutants and the wild type were equally fertile when used as males in sexual crosses, ...	2007	17304613
occurrence of fumonisins b1 and b2 in portuguese maize and maize-based foods intended for human consumption.	fumonisin b(1) (fb(1)) and fumonisin b(2) (fb(2)) are mycotoxins mainly produced by fusarium verticillioides and fusarium proliferatum, which are field pathogens of maize. a survey was conducted on the incidences of fb(1) and fb(2) in both maize and derived products purchased in portugal. the analytical method involved extraction with methanol-water, clean-up by immunoaffinity column and derivatization with naphthalene-2,3-dicarboxaldehyde. determination was carried out by high-performance liqui ...	2007	17454111
isolation and properties of a levo-lactonase from fusarium proliferatum ecu2002: a robust biocatalyst for production of chiral lactones.	a fungus strain ecu2002, capable of enantioselectively hydrolyzing chiral lactones to optically pure hydroxy acids, was newly isolated from soil samples through two steps of screening and identified as fusarium proliferatum (matsushima) nirenberg. from the crude extract of f. proliferatum ecu2002, a novel levo-lactonase was purified to homogeneity, with a purification factor of 460-folds and an overall yield of 9.7%, by ultrafiltration, acetone precipitation, and chromatographic separation throu ...	2007	17530243
occurrence of fumonisins b1 and b2 in broa, typical portuguese maize bread.	fumonisin b(1) (fb(1)) and fumonisin b(2) (fb(2)) are mycotoxins mainly produced by fusarium verticillioides, and fusarium proliferatum, fungi species most commonly isolated from maize. the natural occurrence of fb(1) and fb(2) in broa, typical portuguese maize bread, was evaluated in 30 samples. twenty five were found positive with levels ranging from 142 to 550 microg kg(-1). the limit established by the european regulations was exceeded by 27% of the samples. the tolerable daily intake for fu ...	2007	17604863
fumonisins production by fusarium proliferatum strains isolated from asparagus crown.	the present work deals with the capability for producing fumonisin by fusarium proliferatum strains isolated from asparagus in china. fifty of f. proliferatum strains were randomly selected and incubated on cultures of maize grain and asparagus spear, respectively. fumonisin levels (fb(1) and fb(2)) were determined by high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (hplc-esi-ms/ms). the results showed that all 50 strains produced fumonisins in m ...	2007	17629787
genetic factors affecting sexual reproduction in toxigenic fusarium species.	mycotoxin producing capability greatly varies within species. in theory, the major source of this variability is meiotic recombination. however, a number of important toxigenic species have no known sexual stage and, therefore, the origin of the intraspecific diversity in these fungi is poorly understood. mating in sexually reproducing ascomycetes is controlled by mat genes, but fungi with no known sexual stage also may have fully functional, constitutively transcribed mating type genes. the mat ...	2007	17720271
wheat kernel black point and fumonisin contamination by fusarium proliferatum.	fusarium proliferatum is a major cause of maize ear rot and fumonisin contamination and also can cause wheat kernel black point disease. the primary objective of this study was to determine whether nine f. proliferatum strains from wheat from nepal can cause black point and fumonisin contamination in wheat kernels. for comparison, the study included three fusarium strains from us maize. in test 1, all the strains but one produced significant symptoms of kernel black point; two strains decreased ...	2007	17886185
antifungal activity of lactobacillus paracasei ssp. tolerans isolated from a sourdough bread culture.	lactic acid bacteria were isolated from four different sourdough bread cultures previously investigated for antifungal activity. a total of 116 isolates were obtained and screened for antifungal activity against a battery of molds. the most inhibitory isolate obtained was identified by api 50 chl and 16s ribosomal rna genotyping and found to be lactobacillus paracasei ssp. tolerans. this isolate completely inhibited the growth of fusarium proliferatum m 5689, m 5991 and fusarium graminearum r 40 ...	2008	18077044
successful salvage treatment of disseminated cutaneous fusariosis with liposomal amphotericin b and terbinafine after allogeneic stem cell transplantation.	fusarium infections are associated with high mortality after allogeneic stem cell transplantation. we report on successful treatment of a disseminated cutaneous fusarium proliferatum infection using liposomal amphotericin b and terbinafine. in vitro susceptibility tests of antifungal drugs suggest that terbinafine is a potent additional antifungal drug for disseminated cutaneous fusariosis.	2008	18194367
characterization of a new mitochondrial plasmid from fusarium proliferatum.	a 10.3kb linear mitochondrial dna plasmid designated pfp1 was isolated from fusarium proliferatum. the dna sequence of the plasmid consists of 10,336bp with perfect terminal inverted repeats of 400bp. two major, non-overlapping orfs were identified on opposite strands, encoding a phage-type rna polymerase and a family b type dna polymerase, respectively. one additional minor orf encoding a putative highly basic protein was also identified. the copy number of pfp1, as determined by rt-pcr, ranged ...	2008	18262647
antifungal susceptibility profile of clinical fusarium spp. isolates identified by molecular methods.	to analyse the susceptibility pattern of a collection of fusarium clinical isolates.	2008	18263569
a fumonisin biosynthetic gene cluster in fusarium oxysporum strain o-1890 and the genetic basis for b versus c fumonisin production.	most species of fusarium that produce fumonisin mycotoxins produce predominantly b fumonisins (fbs). however, fusarium oxysporum strain o-1890 produces predominantly c fumonisins (fcs). in this study, the nucleotide sequence of the fumonisin biosynthetic gene (fum) cluster in strain o-1890 was determined. the order and orientation of fum genes were the same as in the previously described clusters in fusarium verticillioides and fusarium proliferatum. coding regions of f. oxysporum and f. vertici ...	2008	18375156
fphog1, a hog-type map kinase gene, is involved in multistress response in fusarium proliferatum.	delta fphog1 mutants of fusarium proliferatum obtained by targeted gene disruption of fphog1, an orthologue of the saccharomyces cerevisiae hog1 mapk gene showed increased sensitivity towards different abiotic stressors including uv-irradiation, heat, salt, osmotic and hydrogen peroxide treatments. incubation of the delta fphog1 mutants under hyperosmotic conditions was accompanied with prolonged growth arrest, inhibition of conidial germination, morphological abnormalities and time-dependent in ...	2008	18506895
the use of its dna sequence analysis and maldi-tof mass spectrometry in diagnosing an infection with fusarium proliferatum.	although mycoses are among the most common diseases worldwide, infections with fusarium spp. occur only rarely. mostly patients suffering from underlying immune deficiency are infected with this mould, resulting in a considerably decreasing prognosis. in immunocompromised patients, cutaneous manifestations are more often associated with fusarium sp. than with candida sp. or aspergillus sp. we describe one patient with acute lymphoblastic leukaemia, who was first treated with chemotherapy after g ...	2008	18547323
metabiotic effects of fusarium spp. on escherichia coli o157:h7 and listeria monocytogenes on raw portioned tomatoes.	the metabiotic effects of fusarium proliferatum, f. avenaceum, and f. oxysporum on escherichia coli o157:h7 and listeria monocytogenes in fresh tomatoes were investigated. tomatoes were preinoculated with the molds and incubated at 15 degrees c for 7 days; then they were inoculated separately with the pathogens, packaged in air and modified atmosphere (5% o2, 30% co2, and 65% n2), and stored at 4, 8, and 12 degrees c for 9 days. the cell loads of pathogens and lactic acid bacteria and the ph wer ...	2008	18680934
production of gibberellic acids by an orchid-associated fusarium proliferatum strain.	the rice pathogen fusarium fujikuroi is well known for its ability to produce the plant hormones gibberellins (gas). however, the majority of closely related fusarium species is unable to produce gas although the ga gene cluster is present in their genomes. in this study, we analyzed five orchid-associated fusarium isolates for their capacity to produce gas. four of them did not produce any gas and were shown not to contain any ga biosynthetic genes. however, the fifth isolate, which has been id ...	2008	18694840
mango malformation disease and the associated fusarium species.	abstract mango malformation disease (mmd) occurs in asia, africa, and the americas and was first reported in india in 1891. the vegetative form of mmd was first reproduced in 1966 with fusarium moniliforme and the floral form with isolates of f. moniliforme var. subglutinans from both vegetative shoots and floral tissue. the fungi were subsequently recognized as f. subglutinans. in 2002, a new species, f. mangiferae, was established based on nuclear and mitochondrial dna sequences; it included s ...	2006	18943188
isolation and characterization of a cold-tolerant strain of fusarium proliferatum, a biocontrol agent of grape downy mildew.	abstract a cold-tolerant strain of the mycoparasite fusarium proliferatum was isolated following uv mutagenesis of the g6 strain, which is a biocontrol agent of grape downy mildew. the isolated strain (designated 1505) exhibited radial growth two to threefold that of the parent strain when grown at 13 degrees c, which is generally suboptimal for growth of fusarium spp., but desirable for its host, plasmopara viticola. this rapid growth was correlated with improved biological control of p. vitico ...	2001	18943441
antifungal activity of lactobacillus paracasei subsp. tolerans against fusarium proliferatum and fusarium graminearum in a liquid culture setting.	lactobacillus paracasei subsp. tolerans, isolated from a traditional sourdough bread culture and previously shown to have antifungal activity against fusarium species, was tested for inhibition of growth of fusarium proliferatum m 5991 and m 5689 and f. graminearum r 4053 in a liquid medium setting. this isolate completely inhibited the growth of f. proliferatum m 5689 and m 5991 and f. graminearum r 4053, whereas such growth was not inhibited in the control in a supernatant agar plate assay. wh ...	2008	19044263
synergistic degradation of 2-naphthol by fusarium proliferatum and bacillus subtilis in wastewater.	2-naphthol, which originates widely from various industrial activities, is toxic and thus harmful to human liver and kidney. a new compound biodegradation system was adopted to degrade 2-naphthol-contaminated wastewater. enzymatic response to 2-naphthol biodegradation in the aqueous phase was also studied. as a co-metabolic substrate, salicylic acid could induce the two microorganisms to produce a large amount of degradation enzymes for 2-naphthol. the key enzymes were confirmed as polyphenol ox ...	2009	19070430
n-starvation stress induced fum gene expression and fumonisin production is mediated via the hog-type mapk pathway in fusarium proliferatum.	during cultivation of a wild type strain of fusarium proliferatum on ammonium dihydrogen phosphate containing defined medium, expression levels of fum1 and fum8, members of the fumonisin biosynthesis gene cluster significantly increased when ammonium ion concentration of the culture medium decreased below 10 mm, indicating that n-depletion triggers the fumonisin biosynthesis genes. deletion of fphog1, a hog-type map kinase gene resulted in further increases in fum1 and fum8 expression under nitr ...	2009	19181411
less-frequent fusarium species of clinical interest: correlation between morphological and molecular identification and antifungal susceptibility.	forty-eight fusarium isolates morphologically identified as belonging to seven species of clinical interest (i.e., fusarium chlamydosporum, fusarium dimerum, fusarium incarnatum, fusarium napiforme, fusarium nygamai, fusarium proliferatum, and fusarium sacchari) were characterized molecularly by the analysis of the sequences of the tub region of the beta-tubulin gene. f. chlamydosporum and f. dimerum were the most genetically heterogeneous species. a high degree of correlation between the morpho ...	2009	19321723
fumonisins, trichothecenes and zearalenone in cereals.	fumonisins are phytotoxic mycotoxins which are synthesized by various species of the fungal genus fusarium such as fusarium verticillioides (sacc.) nirenberg (ex f.moniliforme sheldon) and fusarium proliferatum. the trichothecene (tc) mycotoxins are secondary metabolites produce by species that belong to several fungal genera, especially fusarium, stachybotrys, trichothecium, trichoderma, memnoniella and myrothecium. fusarium mycotoxins are widely dispersed in cereals and their products. zearale ...	2008	19330061
fumonisin b(1) production by fusarium proliferatum strains isolated from allium fistulosum plants and seeds in japan.	to test the fumonisin b(1)-producing ability of fusarium proliferatum strains isolated from welsh onion (allium fistulosum) plants and seeds of commercial cultivars in japan and to examine the applicability of pcr-based assays to discriminate between fumonisin b(1)-producing and nonproducing isolates.	2009	19416462
use of mass spectrometry to identify clinical fusarium isolates.	fusarium spp. have recently emerged as significant human pathogens. identification of these species is important, both for epidemiological purposes and for patient management, but conventional identification based on morphological traits is hindered by major phenotypic polymorphism. in this study, 62 strains, or isolates, belonging to nine fusarium species were subjected to both molecular identification tef1 gene sequencing and matrix-assisted laser desorption ionization-time-of-flight (maldi-to ...	2009	19456834
antifungal activity, mechanism and qsar studies on chalcones.	in this paper, the synthesis of 48 chalcones and their in vitro antifungal activity against aspergillus flavus ncim 594, fusarium proliferatum ncim1105, aspergillus niger ncim 596 and candida tropicalis ncim 3556 are reported. predominantly, the active compounds (6, 9, 14, 27, and 47) against all four fungi have found to have electron withdrawing substitutions in their para substitution in b-ring. quantitative structure-activity relationship indicated that the antifungal activities are correlate ...	2009	19519746
the its region as a taxonomic discriminator between fusarium verticillioides and fusarium proliferatum.	the maize pathogens fusarium verticillioides (fv) and fusarium proliferatum (fp) are morphologically very similar to one another, so fp isolates have been often mistaken as fusarium moniliforme (the former name of fv). the only presently accepted morphological discriminator between these species is the presence/absence of polyphialides. here, a collection of 100 fusarium strains, isolated from infected maize kernels on plants grown in north-western italy, were assigned as fv or fp on the basis o ...	2009	19631741
electrochemical and afm characterization on gold and carbon electrodes of a high redox potential laccase from fusarium proliferatum.	the redox potential of the t1 copper site of laccase from fusarium proliferatum was determined by titration to be about 510 mv vs. sce (750 mv vs. nhe), which makes it a high redox potential enzyme. anaerobic electron transfer reactions between laccase and carbon and gold electrodes were detected, both in solution and when the enzyme was adsorbed on these surfaces. in solution, a single high-potential signal (660 mv vs. sce) was recorded at the carbon surfaces, attributable to the t1 copper site ...	2010	19854115
biocatalytic properties of a recombinant fusarium proliferatum lactonase with significantly enhanced production by optimal expression in escherichia coli.	the levo-lactonase gene of fusarium proliferatum ecu2002 (ec3.1.1.25) was cloned and expressed in escherichia coli jm109 (de3) for biocatalytic resolution of industrially important chiral lactones, including dl-pantoyl lactone which was a key precursor to calcium d-pantothenate. by increasing the biomass concentration and lowering the inducer (isopropyl-beta-d-thiogalactoside) concentration and induction temperature, the lactonase production was significantly enhanced up to 20 ku/l, which was 20 ...	2010	19876606
synthesis of n-alkyl-beta-d-glucosylamines and their antimicrobial activity against fusarium proliferatum, salmonella typhimurium, and listeria innocua.	in this study, different n-alkyl-beta-d-glucosylamines were evaluated for both antifungal and antibacterial activity against fusarium proliferatum (inra, mucl 1807.7), listeria innocua (istab, université bordeaux 1), and salmonella typhimurium (institut pasteur 5858). the tested glucosylamines were beta-d-glucosylamine (gpa), n-ethyl-beta-d-glucosylamine (etgpa), n-butyl-beta-d-glucosylamine (bugpa), n-hexyl-beta-d-glucosylamine (hegpa), n-octyl-beta-d-glucosylamine (ocgpa), n-dodecyl-beta-d-glu ...	2009	19908897
genetic variability and fumonisin production by fusarium proliferatum.	fusarium proliferatum is together with fusarium verticillioides the main source of fumonisins, a health risk mycotoxin, contaminating agro-products. contrary to f. verticillioides, it colonizes a wide range of host plants besides maize, such as wheat or barley among others, in particular in certain regions (southern europe). the phylogenetic study performed in this work using a wide sample of isolates from diverse hosts and origins revealed a high variability, while no host preferences could be ...	2010	19913692
fusarium proliferatum soft tissue infection at the site of a puncture by a plant: recovery, isolation, and direct molecular identification.	after allogeneic stem cell transplantation, a 49-year-old man developed fever and inflammation at the site of a plant puncture on a finger. a hyalohyphomycete was recovered by incubating the plant spine fragment following surgery. amplification of the internal transcribed spacer region and 5.8s rrna, beta-tubulin, and translation elongation factor coding genes identified fusarium proliferatum, which was confirmed later by culture.	2010	19923491
adenylyl cyclase regulates heavy metal sensitivity, bikaverin production and plant tissue colonization in fusarium proliferatum.	a homologue of the adenylyl cyclase (ac) gene of neurospora crassa, named fpacy1 was cloned from the genomic library of fusarium proliferatum item 2287 by screening the library with a dna fragment amplified by using pcr primers designed from conserved sequences of the catalytic domain of ac genes from other fungi. the deduced fpacy1 protein had 53-77% identity with the ac proteins of other fungi. deltafpacy1 mutants obtained by targeted gene disruption showed retarded vegetative growth, increase ...	2010	20082366
time-lapse analysis of cell death in mammalian and fungal cells.	time-lapse video microscopy was designed to follow the movement of single cells for an unlimited period of time under physiological conditions. the system is based on two inverted microscopes located in a co(2) incubator and equipped with charge-coupled device cameras connected to the computer. frames were recorded every minute and the subsequent video sequence was converted to database form. the system was applied to describe the movements of normal hacat cells and pb-treated cells causing the ...	2010	20218896
antibacterial effect of the bioactive compound beauvericin produced by fusarium proliferatum on solid medium of wheat.	to obtain the bioactive compound beauvericin (bea), fusarium proliferatum cect 20569 was grown on a solid medium of wheat, utilizing the technique of the solid state fermentation (ssf), being this mycotoxin purified by high performance liquid chromatography (hplc) with a reverse phase semi-preparative column using as the mobile phase acetonitrile/water in gradient condition. the purity of the bea was verified by analytical hplc and liquid chromatography tandem mass spectrometry (lc/ms-ms). the p ...	2010	20371252
antifungal effects of the bioactive compounds enniatins a, a(1), b, b(1).	to produce enniatin (ens), fusarium tricinctum cect 20150 was grown in a liquid medium of potato (pdb), being mycotoxin purified by high performance liquid chromatography (hplc) with a reverse phase semipreparative column using a mobile phase of acetonitrile/water using gradient condition. the purity of the ens fractions was verified by analytical hplc and lc/ms-ms. the pure fractions of ens were utilized to study the biological activity on several mycotoxigenic moulds as fusarium verticilloides ...	2010	20417654
formation of fumonisins and other secondary metabolites by fusarium oxysporum and f. proliferatum: a comparative study.	the principal aim of this study was to estimate the formation of fumonisins (fb(1) and fb(2)), moniliformin (mon), and ergosterol (erg) by fusarium oxysporum and fusarium proliferatum, while the formation of beauvericin (bea) was estimated by the latter fusarium species only. moreover, the effect of temperature on the biosynthesis of mycotoxins was also evaluated. fumonisins were formed by f. proliferatum, with the highest yield at 18 degrees c (720.0-1976.6 microg g(-1) for fb(1), 74.2-670.8 mi ...	2010	20455157
characterization of glycolipid biosurfactant from pseudomonas aeruginosa cpcl isolated from petroleum-contaminated soil.	to isolate and characterize the biosurfactant-producing micro-organism from petroleum-contaminated soil as well as to determine the biochemical properties of the biosurfactant.	2010	20477962
differential effect of environmental conditions on the growth and regulation of the fumonisin biosynthetic gene fum1 in the maize pathogens and fumonisin producers fusarium verticillioides and fusarium proliferatum.	the effects of ecophysiological factors, temperature and solute potential, on both the growth and the regulation of the fumonisin biosynthetic fum1 gene were studied and compared in one isolate each of the two closely related fumonisin-producing and maize pathogens fusarium verticillioides and fusarium proliferatum. the effect of solute potential and temperature was examined on in vitro mycelia growth and on the expression of the fum1 gene, quantified by species-specific real-time reverse transc ...	2010	20491926
potential of pediococcus pentosaceus (l006) isolated from maize leaf to suppress fumonisin-producing fungal growth.	the present study was aimed at characterizing the ability of lactic acid bacteria isolated from maize to repress the growth of fumonisin-producing fungi. a total of 67 isolates were screened for their antifungal activity against fusarium proliferatum and fusarium verticillioides by using the overlay method. the most efficient antifungal isolate was identified as pediococcus pentosaceus (l006), on the basis of physiological and biochemical characterization and 16s rrna gene sequencing. production ...	2010	20537273
hydroxylation at carbon-2 of ent-16-oxo-17-norkauran-19-oic acid by fusarium proliferatum.	a new product of biotransformation of ent-16-oxo-17-norkauran-19-oic acid (1) by fusarium proliferatum was isolated and identified as a 2beta-hydroxy derivative (2). the structure of 2 was elucidated on the basis of spectroscopic data interpretation and single-crystal x-ray diffraction analysis. the allelopathic activity of compound 2 was evaluated on the growth of radicals and shoots of lactuca sativa (lettuce). this is the first time that fungal hydroxylation at position c-2 has been reported ...	2010	20617817
fumonisin b(1) and implications in nursery swine productivity: a quantitative exposure assessment.	this study estimated the long-term exposure of fumonisin b(1) (fb(1)) in nursery swine diets and associated toxicological adverse effects on negative productivity potential using quantitative exposure assessment. fumonisin b(1) is a mycotoxin produced by fusarium verticillioides and fusarium proliferatum and is a common biological contaminant of corn (zea mays l.) and other grains. acute effects from fb(1) exposures are well recognized and managed in the swine industry, but practices to limit pr ...	2010	20622179
production and migration of mycotoxins in sweet pepper analyzed by multimycotoxin lc-ms/ms.	in this work the presence and migration behavior of mycotoxins formed in sweet pepper, inoculated by fusarium species involved in internal fruit rot, were investigated. two different commercial sweet pepper cultivars were inoculated with two different fusarium proliferatum isolates that were sampled from diseased peppers. after 10 days of incubation at 20 °c in a closed container, the lesion caused by the fungal infection was dissected. around the lesion, up to three concentric rings of pepper f ...	2010	20825158
a molecular based strategy for rapid diagnosis of toxigenic fusarium species associated to cereal grains from argentina.	fusarium species are worldwide causal agents of ear rot in cereals. their toxigenic potential is a health risk for both humans and animals. in argentina, most identification of these fungi has been based on morphological and cross-fertility criteria which are time consuming and require considerable expertise in fusarium taxonomy and physiology. dna based approaches have been reported as rapid, sensitive and specific alternatives to identify the main fumonisin and trichothecene-producing fusarium ...	2010	20965064
dermatitis in captive wyoming toads (bufo baxteri) associated with fusarium spp.	from may 2007 to june 2008, 30 of 49 wyoming toads (bufo baxteri) kept at omaha's henry doorly zoo (nebraska, usa) died showing clinical signs of ventral erythema, inappetance, lethargy, and delayed righting reflex. treatment with antifungals and antibiotics was unsuccessful in all cases. histopathologic analyses revealed dermatitis as the primary problem in 20 of 21 toads in which skin was examined. fungal dermatitis was present in 17 toads, with hyphae approximately 1-3 μm in diameter, and par ...	2010	20966269
sphaerodes mycoparasitica biotrophic mycoparasite of 3-acetyldeoxynivalenol- and 15-acetyldeoxynivalenol-producing toxigenic fusarium graminearum chemotypes.	fusarium spp. are economically important crop pathogens and causal agents of fusarium head blight (fhb) of cereals worldwide. of the fhb pathogens, fusarium graminearum 3-acetyldeoxynivalenol (3-adon) and 15-acetyldeoxynivalenol (15-adon) are the most aggressive mycotoxigenic chemotypes, threatening food and feed quality as well as animal and human health. the objective of the study was to evaluate host specificity and fungal-fungal interactions of sphaerodes mycoparasitica- a recently described ...	2011	21204935
in vitro inhibitory effect of tetrahydrocurcuminoids on fusarium proliferatum growth and fumonisin b₁ biosynthesis.	many plant pathogens produce toxic metabolites when growing on food and feed. some antioxidative components seem to prevent fungal growth and mycotoxin formation. recently, we synthesized a new class of powerful antioxidative compounds, i.e. tetrahydrocurcuminoids, and its structure/antioxidant activity relationships have been established. the south west of france produces large amounts of corn, which can be infected by fusarium species, particularly f. proliferatum. in this context, the efficie ...	2011	21240822
fum cluster divergence in fumonisins-producing fusarium species.	fumonisins are polyketide-derived mycotoxins, produced by several fusarium species, and its biosynthetic pathway is controlled by the fum cluster--a group of genes exhibiting a common expression pattern during fumonisin biosynthesis. the most common are the b analogues with fumonisin b(1) (fb(1)) being the most prevalent. at least a part of the inter- and intraspecific variation in fbs synthesis level can be explained by the sequence differences inside fum cluster. the aim of our study was to ev ...	2010	21315309
production of dragon's blood in dracaena cochinchinensis plants by inoculation of fusarium proliferatum.	dragon's blood is a traditional medicine widely used in the world from ancient times. however, little is known about its formation mechanism. this work aimed to gain some insights into its formation mechanism and to control its production. the results demonstrate that wounding plus causal fungal infection and keeping the wound moist are essential for efficient dragon's blood formation in dracaena cochinchinensis. two fungal isolates ym-266 and ym-71213 of fusarium proliferatum increased the yiel ...	2011	21421373
determination of the loq in real-time pcr by receiver operating characteristic curve analysis: application to qpcr assays for fusarium verticillioides and f. proliferatum.	real-time pcr (qpcr) is the principal technique for the quantification of pathogen biomass in host tissue, yet no generic methods exist for the determination of the limit of quantification (loq) and the limit of detection (lod) in qpcr. we suggest using the youden index in the context of the receiver operating characteristic (roc) curve analysis for this purpose. the loq was defined as the amount of target dna that maximizes the sum of sensitivity and specificity. the lod was defined as the lowe ...	2011	21603916
persistent fingernail onychomycosis caused by fusarium proliferatum in a healthy woman.		2011	21668519
disruption of sphingolipid metabolism and induction of equine leukoencephalomalacia by fusarium proliferatum culture material containing fumonisin b(2) or b(3).	fumonisin b(1), b(2), and b(3) are inhibitors of ceramide synthase, a key enzyme in the pathway for de novo sphingolipid biosynthesis. corn, naturally contaminated with either predominantly fumonisin b(1) or pure fumonisin b(1), has been shown to cause equine leukoencephalomalacia (elem). it has been hypothesized that fumonisin-induced disruption of sphingolipid metabolism is an early event in the development of elem. recently, it was shown that fusarium proliferatum corn culture diets containin ...	1997	21781781
genetic and phenotypic variation of fusarium proliferatum isolates from different host species.	fusarium proliferatum (matsushima) nirenberg is a common pathogen infecting numerous crop plants and occurring in various climatic zones. it produces large amounts of fumonisins, a group of polyketide-derived mycotoxins. fumonisin biosynthesis is determined by the presence and activity of the fum cluster, several co-regulated genes with a common expression pattern. in the present work, we analyzed 38 f. proliferatum isolates from different host plant species, demonstrating host-specific polymorp ...	2011	21796391
in vitro antimicrobial and antioxidant activity of acetone and methanol extracts from thymus leucotrichius (lamiaceae).	thymus species has been used as tonic and herbal tea, antiseptic, antitussive, carminative, antioxidant, anti-inflammatory and antimicrobial activities. the acetone and methanol extracts of thymus (t.) leucotrichius (labiatae/lamiaceae) was examined for antimicrobial and antioxidant properties.	2011	21796869
Fusarium proliferatum, an endophytic fungus from Dysoxylum binectariferum Hook.f, produces rohitukine, a chromane alkaloid possessing anti-cancer activity.	Rohitukine is a chromane alkaloid possessing anti-inflammatory, anti-cancer and immuno-modulatory properties. The compound was first reported from Amoora rohituka (Meliaceae) and later from Dysoxylum binectariferum (Meliaceae) and Schumanniophyton problematicum (Rubiaceae). Flavopiridol, a semi-synthetic derivative of rohitukine is a potent CDK inhibitor and is currently in Phase III clinical trials. In this study, the isolation of an endophytic fungus, Fusarium proliferatum (MTCC 9690) from the ...	2011	21898150
free radicals, salicylic acid and mycotoxins in asparagus after inoculation with fusarium proliferatum and f. oxysporum.	electron paramagnetic resonance was used to monitor free radicals and paramagnetic species like fe, mn, cu generation, stability and status in asparagus officinalis infected by common pathogens fusarium proliferatum and f. oxysporum. occurrence of f. proliferatum and f. oxysporum, level of free radicals and other paramagnetic species, as well as salicylic acid and mycotoxins content in roots and stems of seedlings were estimated on the second and fourth week after inoculation. in the first term ...	2011	21957331
identification and functional characterization of indole-3-acetamide-mediated iaa biosynthesis in plant-associated fusarium species.	the plant hormone indole-3-acetic acid (iaa) can be synthesized from tryptophan via the intermediate indole-3-acetamide (iam). the two genes, iaam (encoding tryptophan monooxygenase) and iaah (encoding indole-3-acetamide hydrolase) that constitute the iam pathway have been described in plant-associated bacteria. we have identified putative homologs of the bacterial iaam and iaah genes in four fusarium species -fusarium proliferatum, fusarium verticillioides, fusarium fujikuroi, and fusarium oxys ...	2011	22079545
a luminescent bacterium assay of fusaric acid produced by fusarium proliferatum from banana.	fusarium proliferatum was isolated as a major pathogen causing the fusarium disease in harvested banana fruit. one of its major compounds, fusaric acid, was identified by high-performance liquid chromatography-electrospray ionization mass spectrometry (hplc-esi-ms). because the light intensity of the luminescent bacterium vibrio qinghaiensis sp. nov. q67 can be inhibited by fusaric acid, the fusaric acid content of f. proliferatum was assessed and compared by both the hplc and luminescent bacter ...	2012	22105299
influence of light on growth, fumonisin biosynthesis and fum1 gene expression by fusarium proliferatum.	fumonisins are a group of mycotoxins, mainly found in maize and maize-based food and feed, associated with several diseases in animals. the impact of these toxins on the economy and health worldwide has driven several efforts to clarify the role of environmental factors that can influence fumonisin biosynthesis by the toxigenic species. we analyzed the influence of light of varying wavelength on growth and fumonisin biosynthesis by the fungus fusarium proliferatum item 1719. light in general had ...	2011	22137249
the role of mycelium production and a mapk-mediated immune response in the c. elegans-fusarium model system.	fusariosis is an emerging infectious complication of immune deficiency, but models to study this infection are lacking. the use of the soil nematode caenorhabditis elegans as a model host to study the pathogenesis of fusarium spp. was investigated. we observed that fusarium conidia consumed by c. elegans can cause a lethal infection and result in more than 90% killing of the host within 120 hours, and the nematode had a significantly longer survival when challenged with fusarium proliferatum com ...	2012	22225407
in vitro antifungal activity of terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol against mycotoxigenic plant pathogens.	the aim of this study was to examine the effect of five naturally occurring compounds from essential oils on 10 different species of mycotoxigenic fungi involved in several plant diseases. the antifungal activities of terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol were observed in vitro on fusarium subglutinans, fusarium cerealis, fusarium verticillioides, fusarium proliferatum, fusarium oxysporum, fusarium sporotrichioides, aspergillus tubingensis, aspergillus carbonarius, ...	2011	22257275
the fus3/kss1-type map kinase gene fpk1 is involved in hyphal growth, conidiation and plant infection of fusarium proliferatum.	fusarium proliferatum is an important pathogen of maize that is responsible for ear rots, stalk rots and seeding blight worldwide. during the past decade, f. proliferatum has caused several severe epidemics of maize seedling blight in many areas of china, which led to significant losses in maize. to understand the molecular mechanisms in the fungal developmental regulation and pathogenicity, we isolated and characterized the fpk1 gene (genbank accession no. hq844224) encoding a map kinase homolo ...	2011	22286038
endophytic fungi from pigeon pea [cajanus cajan (l.) millsp.] produce antioxidant cajaninstilbene acid.	in this study, novel endophytic fungi producing cajaninstilbene acid (csa) from pigeon pea [ cajanus cajan (l.) millsp.] were investigated and screened. csa has prominent pharmacological activities. a total of 110 endophytic fungi isolates were grouped into 8 genera on the basis of morphological characteristics, and csa-producing fungi were screened by liquid chromatography-tandem mass spectrometry (lc-ms/ms). according to its-rdna sequences analysis, the csa-producing fungi were identified as f ...	2012	22494407
analysis of fumonisin contamination and the presence of fusarium in wheat with kernel black point disease in the united states.	the ability of the fungus fusarium proliferatum to cause kernel black point disease in wheat was previously established, but natural contamination of black point wheat with both f. proliferatum and fumonisin mycotoxins has not been studied in the united states. low levels of fumonisins were detected in nine of 43 wheat samples with kernel black point disease that were obtained from across the united states. a subset of samples was contaminated with f. proliferatum as well as with f. fujikuroi, f ...	2012	22494515
experimental infection of fusarium proliferatum in oryza sativa plants; fumonisin b1 production and survival rate in grains.	fusarium proliferatum is a plant pathogenic fungus associated with crops such as asparagus and corn, and it possesses the ability to produce a range of mycotoxins, including fumonisins. in asia, rice (oryza sativa) is a staple cereal and is occasionally colonized by this fungus without obvious physiological changes. f. proliferatum is closely related to gibberella fujikuroi (anamorph f. fujikuroi) responsible for bakanae disease in rice; however there are few reports of f. proliferatum as a rice ...	2012	22534354
in vitro antifungal susceptibility of filamentous fungi causing rare infections: synergy testing of amphotericin b, posaconazole and anidulafungin in pairs.	mucormycetes (formerly known as zygomycetes of the order mucorales) and hyaline moulds such as those of the genus fusarium or paecilomyces are emerging as significant human pathogens. the aim of the study was to determine the in vitro antifungal susceptibility of these fungi to older and newer antifungals and to investigate the antifungal activity of amphotericin b, posaconazole and anidulafungin in dual combinations.	2012	22535624
bromelain, a cysteine protease from pineapple (ananas comosus) stem, is an inhibitor of fungal plant pathogens.	this study aimed to evaluate the effect of bromelain, a cysteine protease isolated from pineapple (ananas comosus), on growth of several agronomically important fungal pathogens.	2012	22537505
segregation of secondary metabolite biosynthesis in hybrids of fusarium fujikuroi and fusarium proliferatum.	fusarium fujikuroi and fusarium proliferatum are two phylogenetically closely related species of the gibberella fujikuroi species complex (gfc). in some cases, strains of these species can cross and produce a few ascospores. in this study, we analyzed 26 single ascospore isolates of an interspecific cross between f. fujikuroi c1995 and f. proliferatum d4854 for their ability to produce four secondary metabolites: gibberellins (gas), the mycotoxins fusarin c and fumonisin b(1), and a family of re ...	2012	22626844
benefit of polyhexamethylene biguanide in fusarium keratitis.	to report a series of 3 patients with soft contact lens-related fusarium keratitis. two of them were treated with the antiamoebic polyhexamethylene biguanide 0.02% (phmb) in combination with antifungal drugs, and 1 patient was treated with phmb as sole antifungal regimen.	2012	22710976
antioxidant capacity, antimicrobial activity and triterpenes isolated from jatropha isabellei müll arg.	jatropha isabellei müll arg. belongs to the family euphorbiaceae. the antioxidant capacity of this plant was evaluated by the dpph method and inhibition of thiobarbituric acid reactive substances (tbars). ethyl acetate fraction demonstrated the best antioxidant capacity against the dpph free radical and its profile was similar to ascorbic acid. this fraction also presented the best results of phenolics, flavonoids and condensed tannins, showing a positive relation between the phenolic content an ...	2013	22788721
comparison of antifungal activities of vietnamese citrus essential oils.	citrus essential oils (eos) are volatile compounds from citrus peels and widely used in perfumes, cosmetics, soaps and aromatherapy. in this study, inhibition of citrus eos extracted from vietnamese orange (citrus sinensis), mandarin (citrus reticulata blanco), pomelo (citrus grandis osbeck) and lime (citrus aurantifolia swingle) on the growth of plant pathogenic fungi, mucor hiemalis, penicillium expansum and fusarium proliferatum was investigated. the eos of the citrus peels were obtained by c ...	2013	22799453
near-infrared (nir) hyperspectral imaging and multivariate image analysis to study growth characteristics and differences between species and strains of members of the genus fusarium.	near-infrared (nir) hyperspectral imaging was used to study three strains of each of three fusarium spp. (fusarium subglutinans, fusarium proliferatum and fusarium verticillioides) inoculated on potato dextrose agar in petri dishes after either 72 or 96 h of incubation. multivariate image analysis was used for cleaning the images and for making principal component analysis (pca) score plots and score images and local partial least squares discriminant analysis (pls-da) models. the score images, ...	2012	22903431
cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer fusarium proliferatum lf061.	the ketoisovalerate reductase (ec 1.2.7.7 ) is required for the formation of beauvericin via the nonribosomal peptide synthetase biosynthetic pathway. it catalyzes the nadph-specific reduction of ketoisovaleric acid to hydroxyisovalerate. however, little is known about the bioinformatics' data about the 2-kiv reductase in fusarium. to date, heterologous production of the gene kivrfp from fusarium has not been achieved.	2012	22916830
the production of β-glucosidases by fusarium proliferatum nbrc109045 isolated from vietnamese forest.	fusarium proliferatum nbrc109045 is a filamentous fungus isolated from vietnamese forest due to high production of β-glucosidases. production of the enzyme was studied on varied carbon source based mediums. the highest activity was obtained in medium containing 1% corn stover + 1% wheat bran (3.31 ± 0.14 u/ml). it is interesting to note that glucose (0.69 ± 0.02 u/ml) gave higher activity and just followed by cellobiose among the di- and mono-saccharides, which is generally regarded as a univers ...	2012	22974424
a multiplex real-time pcr method using hybridization probes for the detection and the quantification of fusarium proliferatum, f. subglutinans, f. temperatum, and f. verticillioides.	maize contamination with fusarium species is one of the major sources of mycotoxins in food and feed derivates. in the present study, a lightcycler(®) real-time pcr method using hybridization probes was developed for the specific identification, detection, and quantification of fusarium proliferatum, fusarium subglutinans, fusarium temperatum, and fusarium verticillioides, four mycotoxin-producing pathogens of maize. primers and hybridization probes were designed to target the translation elonga ...	2012	23063186
biosynthesis of nicotinic acid from 3-cyanopyridine by a newly isolated fusarium proliferatum zjb-09150.	in this study, nitriles were used as sole sources of nitrogen in the enrichments to isolate nitrile-converting microorganisms. a novel fungus named zjb-09150 possessing nitrile-converting enzymes was obtained with 3-cyanopyridine as sole source of nitrogen, which was identified by morphology, biology and 18s rdna gene sequence as fusarium proliferatum. it was found that f. proliferatum had ability to convert nitriles to corresponding acids or amides and showed wide substrate specificity to aliph ...	2013	23085954
cloning, bioinformatics and the enzyme activity analyses of a phenylalanine ammonia-lyase gene involved in dragon's blood biosynthesis in dracaena cambodiana.	phenylalanine ammonia-lyase (pal) is the key enzyme of the phenylpropanoid pathway, playing an important role in plant development and defence. we cloned a partial cdna of pal gene, dcpal1, from dracaena cambodiana seedlings using rt-pcr with degenerate primers that were designed based on a multiple sequence alignment of known pal genes from other plant species. dcpal1 shows highly homologous to other known pal genes registered in genbank, being closest to that of musa acuminata. dcpal1 has a re ...	2013	23108992
fungal his-tagged nitrilase from gibberella intermedia: gene cloning, heterologous expression and biochemical properties.	nitrilase is an important member of the nitrilase superfamiliy. it has attracted substantial interest from academia and industry for its function of converting nitriles directly into the corresponding carboxylic acids in recent years. thus nitrilase has played a crucial role in production of commercial carboxylic acids in chemical industry and detoxification of nitrile-contaminated wastes. however, conventional studies mainly focused on the bacterial nitrilase and the potential of fungal nitrila ...	2012	23226336
wetting properties of fungi mycelium alter soil infiltration and soil water repellency in a γ-sterilized wettable and repellent soil.	soil water repellency (swr) has a drastic impact on soil quality resulting in reduced infiltration, increased runoff, increased leaching, reduced plant growth, and increased soil erosion. one of the causes of swr is hydrophobic fungal structures and exudates that change the soil-water relationship. the objective of this study was to determine whether swr and infiltration could be manipulated through inoculation with fungi. the effect of fungi on swr was investigated through inoculation of three ...	2012	23245615
synergysm of voriconazole or itraconazole with other antifungal agents against species of fusarium.	infections caused by fusarium are difficult to treat because these fungi show in vitro and in vivo resistance to practically all the antifungal agents available, which explains the high mortality rates. an attempt to overcome fungal resistance is the combination of antifungal agents, especially those with different mechanisms of action.	2016	23402831
endophytic fungi isolated from oil-seed crop jatropha curcas produces oil and exhibit antifungal activity.	jatropha curcas l., a perennial plant grown in tropics and subtropics is popularly known for its potential as biofuel. the plant is reported to survive under varying environmental conditions having tolerance to stress and an ability to manage pest and diseases. the plant was explored for its endophytic fungi for use in crop protection. endophytic fungi were isolated from leaf of jatropha curcas, collected from new delhi, india. four isolates were identified as colletotrichum truncatum, and other ...	2013	23409154
isolation and characterization of gibberella intermedia ca3-1, a novel and versatile nitrilase-producing fungus.	nitrilase-mediated biocatalysis has attracted substantial attention for its application in carboxylic acid production in recent years. in the present study, the fungus ca3-1 was isolated and identified as gibberella intermedia based on its morphology, its 18s ribosomal dna (rdna), and internal transcribed spacer (its) sequences. the enzymatic properties of g. intermedia resting cells were determined, and the optimum activity was achieved at 40 °c with ph 7.6. the half-lives of the nitrilase at 3 ...	2013	23457057
diversity of pea-associated f. proliferatum and f. verticillioides populations revealed by fum1 sequence analysis and fumonisin biosynthesis.	fusarium proliferatum and f. verticillioides are considered as minor pathogens of pea (pisum sativum l.). both species can survive in seed material without visible disease symptoms, but still contaminating it with fumonisins. two populations of pea-derived f. proliferatum and f. verticillioides strains were subjected to fum1 sequence divergence analysis, forming a distinct group when compared to the collection strains originating from different host species. furthermore, the mycotoxigenic abilit ...	2013	23470545
maize kernel antioxidants and their potential involvement in fusarium ear rot resistance.	the potential involvement of antioxidants (α-tocopherol, lutein, zeaxanthin, β-carotene, and ferulic acid) in the resistance of maize varieties to fusarium ear rot was the focus of this study. these antioxidants were present in all maize kernel stages, indicating that the fumonisin-producing fungi (mainly fusarium verticillioides and fusarium proliferatum ) are likely to face them during ear colonization. the effect of these compounds on fumonisin biosynthesis was studied in f. verticillioides l ...	2013	23484637
diversity of endophytic fungi and screening of fungal paclitaxel producer from anglojap yew, taxus x media.	endophytic fungi represent underexplored resource of novel lead compounds and have a capacity to produce diverse class of plant secondary metabolites. here we investigated endophytic fungi diversity and screening of paclitaxel-producing fungi from taxus x media.	2013	23537181
occurrence of fumonisins in asparagus (asparagus officinalis l.) and garlic (allium sativum l.) from germany.	fusarium proliferatum is able to produce fumonisins and is considered a pathogen of many economically important plants (e.g. corn, rice, asparagus) [1]. the occurrence of fumonisin fb1 inf. proliferatum infected asparagus spears from germany was investigated using a liquid chromatography/electrospray ionization-mass spectrometry (lc-esi-ms) method with isotopically labeled fumonisin fb1-d6 as internal standard. asparagus samples were harvested in july 2000 and screened forfusarium species. altog ...	2004	23604994
[biocatalytic desymmetric hydrolysis of 3-(4-chlorophenyl)-glutaronitrile to the key precursor of optically pure baclofen].	we produced (s)-4-cyano-3-(4-chlorophenyl)-butyrate by highly stereoselective biocatalyst in this study. a nitrilase-producing strain, named gibberella intermedia wx12, was isolated by 3-(4-chlorophenyl)-glutaronitrile as substrate in the screening with phenol-sodium hypochlorite method. the fermentation conditions and catalytic properties of this strain were investigated. the preferred carbon and nitrogen sources for nitrilase production were lactose (30 g/l) and peptone (20 g/l). after being c ...	2013	23631116
characterization of gibberellin biosynthetic gene cluster from fusarium proliferatum.	gibberellins (gas) are a group of phytohormones that control many developmental processes in higher plants. we report the cloning and expression pattern of gibberellin biosynthesis genes from a new ga-producing fungus, fusarium proliferatum (strain kgl0401). these genes sequences are deposited in the national center for biotechnology information (ncbi) under accession numbers ef119831, ef119832, dq313173, dq313174, dq313175, dq313176, and dq313177. the expression level of these genes was maximal ...	2013	23648850
production of enniatins a, a1, b, b1, b4, j1 by fusarium tricinctum in solid corn culture: structural analysis and effects on mitochondrial respiration.	enniatins (ens) are secondary fungal metabolites with hexadepsipeptidic chemical structure and they possess a number of potent biological activities that can contaminate several kind of food and foodstuffs increasing the exposure risk for consumers. ens are produced by several fusariun strains including fusarium subglutinans, fusarium proliferatum and fusarium tricinctum. production of a mixture of ens was performed by culturing f. tricinctum item 9496 on white corn as substrate. the solid cultu ...	2012	23692767
structural variation and dynamics of the nuclear ribosomal intergenic spacer region in key members of the gibberella fujikuroi species complex.	the intergenic spacer (igs) region of the ribosomal dna was cloned and sequenced in eight species within the gibberella fujikuroi species complex with anamorphs in the genus fusarium, a group that includes the most relevant toxigenic species. dna sequence analyses revealed two categories of repeated elements: long repeats and short repeats of 125 and 8 bp, respectively. long repeats were present in two copies and were conserved in all the species analyzed, whereas different numbers of short repe ...	2013	23706073
fusariotoxins in asparagus - their biosynthesis and migration.	asparagus is often infected by fungi of the fusarium genus, a causal agent of crown and root rot, which decreases the quantity and quality of spears. fusarium oxysporum and fusarium proliferatum are the most severe asparagus pathogens, well known as mycotoxin producers, mainly fumonisins and moniliformin. the present study was undertaken to estimate fumonisin b1, moniliformin and ergosterol concentrations in asparagus tissue. moreover, the possibility of toxin transport to the edible asparagus p ...	2013	23756238
potential effects of environmental conditions on the efficiency of the antifungal tebuconazole controlling fusarium verticillioides and fusarium proliferatum growth rate and fumonisin biosynthesis.	fusarium verticillioides and fusarium proliferatum are important phytopathogens which contaminate cereals in the mediterranean climatic region with fumonisins. in this study we examined the interaction between the fungicide efficacy of tebuconazole and water potential (ψw) (-0.7-7.0mpa)×temperature (20-35°c) on growth and fum1 gene expression by real time rt-pcr (an indicator of fumonisin biosynthesis) in strains of both fusarium species. concentrations of tebuconazole required to reduce growth ...	2013	23800737
cloning and characterization of the gene cluster required for beauvericin biosynthesis in fusarium proliferatum.	beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activities. we isolated and characterized the fpbeas gene cluster, devoted to beauvericin biosynthesis, from the filamentous fungus fusarium proliferatum lf061. targeted inactivation of the f. proliferatum genomic copy of fpbeas abolished the production of beauvericin. comparative sequence analysis of the fpbeas showed 74% similarity with the bbbeas that synthesizes the cyclic ...	2013	23832252
enhancing nitrilase production from fusarium proliferatum using response surface methodology.	the individual and interactive effects of three independent variables i.e. carbon source (glucose), nitrogen source (sodium nitrate) and inducer (ϵ-caprolactam) on nitrilase production from fusarium proliferatum were investigated using design of experiments (doe) methodology. response surface methodology (rsm) was followed to generate the process model and to obtain the optimal conditions for maximum nitrilase production. based on central composite design (ccd) a quadratic model was found to fit ...	2013	23853757
a new isolate from fusarium proliferatum (auf-2) for efficient nitrilase production.	a fungal isolate from fusarium proliferatum strain auf-2 has been found to have a high nitrilase activity (≥ 1,000 u/l culture). the present work describes optimization of growth conditions and production medium to achieve maximum nitrilase production. the most effective carbon and nitrogen sources for nitrilase production were glucose and sodium nitrate, respectively. ε-caprolactam was found to be the best inducer for maximum nitrilase production with 80 g/l wet cell biomass and 26 u/g nitrilas ...	2013	23934068
molecular characterization of pathogenic fusarium species in cucurbit plants from kermanshah province, iran.	fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in kermanshah province, the largest area of cucurbits plantation in iran. therefore, the objectives in this study were to isolate and identify disease-causing fusarium spp. from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships. a total of 100 fusarium isolates were obtained from diseased cucurbit plants collected from fields ...	2011	23961146