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antigens of aspergillus fumigatus. 1. purification of a cytoplasmic antigen reactive with sera of patients with aspergillus-related disease.an antigen has been purified from the mycelial cell sap of aspergillus fumigatus (strain 507). the same component appears to be present in the extracellular phase (culture filtrate) in a partially degraded form. the cell sap protein has a structure composed of four polypeptides of 45,000 daltons linked through disulphide bonds. the isoelectric point (5.2-5.6) and carbohydrate content (12.5% neutral hexose) indicate that this protein is an acidic glycoprotein. it shows reactions with 75% of sera ...19817039876
uncommon causes of peritonitis in patients undergoing peritoneal dialysis.in five patients, peritonitis caused by uncommon agents was acquired during continuous ambulatory peritoneal dialysis. three patients had yeast (two candida albicans, one c parapsilosis), one aspergillus fumigatus, and one nocardia asteroides infections. a review of the literature indicates that yeasts are the most common cause of peritoneal dialysis-associated fungal peritonitis; only one other possible case of aspergillus sp and no nocardia infections have been proved, to our knowledge. observ ...19817004371
prevalence of precipitating antibodies to different extracts of aspergillus fumigatus in a north american asthmatic population.this study was carried out to find the prevalence of precipitin reactions in the sera of 200 north american asthmatic subjects. precipitins were detected by the double diffusion technique using different extracts of aspergillus fumigatus, including a reference 'home produced' extract and five commercial extracts from three different suppliers. in addition, antigenicity of these extracts was assessed by crossed immunoelectrophoresis (xie). of the sera, 13.5% reacted to the reference extract and f ...19817028313
factors influencing the production of cellulase by aspergillus fumigatus (fresenius).during growth in liquid culture containing a single cellulosic or non-cellulosic carbon source, a newly isolated strain of aspergillus fumigatus released cellulases into the medium; the amounts produced depended on the nitrogen source, the type and concentration of the carbon source, ph and temperature. extracellular cellulolytic activity was still increasing after incubation for 60 d with 1% (w/v) cf11 cellulose, (nh4)2so4 as nitrogen source and a starting ph of 7. the activities of the new iso ...19817038036
[progressive cavernous changes in the lungs after pulmonary aspergillosis]. 19817036103
[felty syndrome and abscessing bronchopneumonia caused by aspergillus fumigatus]. 19817036250
terelisa: the elisa test performed in terasaki plates.the use of terasaki (10 microliter samples) and microtitration (100 microliter samples) plates as the solid phase in enzyme immunoassays was compared. various antigens were used for coating the plates and antibodies present in human sera were evaluated using the same anti-human ig antibody labelled with either beta -galactosidase, alkaline phosphatase, peroxidase or glucose oxidase. the results obtained, either by scoring with the naked eye or by absorbance reading with appropriate densitometers ...19817031134
[negative precipitin test after surgical treatment of pulmonary and pleural aspergillosis]. 19817031619
progressive lung disease in a malt-worker.we described a malt-worker whose initial symptoms suggested extrinsic allergic alveolitis. the ensuing cavitating lung disease, aspergillus fumigatus infection with mycetomata, vigorous immune response, and granulomatous liver disease are unusual features.19817031969
fungal flora in the superficial infections of the skin and nails at lucknow. 19817033130
aspergillus keratitis with intraocular invasion.a healthy 48-year-old man developed aspergillus keratitis following mild corneal trauma. intensive medical therapy, initially empirical, then guided by in vitro sensitivity testing, as well as attempts at surgical excision of the infection, were ultimately unsuccessful. the poor therapeutic response may have been due to fungal penetration of the deep corneal stromal before treatment was initiated. the clinical and histologic features of a keratitis are described and related to fungal keratitis i ...19817033854
[otomycosis in the south of chile]. 19817034110
nasal aspergillosis in three horses.three horses were referred for investigation of a unilateral foul smelling scanty nasal discharge, complicated in one case by intermittent epistaxis. thick purulent material or a mycotic plaque was identified by an endoscopic examination of the middle meatus but in two horses this had to be repeated under general anaesthesia before the abnormalities were detected. aspergillus fumigatus was cultured from all three cases and septate hyphae were identified on smears from lesions. histological exami ...19817034361
[the growth of thermophilic fungi strains aspergillus fumigatus and mucor lusitanicus in n-alkane medium (author's transl)].growth parameters of the thermophilic hydrocarbon utilizing strains aspergillus fumigatus and mucor lusitanicus in n-alkanes (kw)- and glucose (gic)-medium in standing and fermenter cultures were investigated. the temperature limits for growth were dependent on the medium composition. the temperature range for sporulation was smaller than for the hyphal growth. both strains were cultivated on carbon limitation with subsequent determinations of n-alkanes glucose, ammoniumsulfate-nitrogen, phospha ...19817034398
[aspergillus endocarditis. report of a case treated surgically with success].a 42 year old man without history of rheumatic heart disease was admitted to the hospital with severe aortic insufficiency and heart failure. there were no clinical data of an infectious disease. the bidimensional echocardiogram showed vegetations involving the aortic valve leaflets. with the diagnosis of acute aortic insufficiency caused by infective endocarditis the patient was submitted to surgery. during surgery the existence of endocarditis secondary to a mycotic agent was demonstrated. the ...19817034662
cultural and nutritional studies of zoopathogenic fungi associated with livestock feeds in nigeria.mycelial growth and nutritional physiology of zoopathogenic aspergillus fumigatus fres., thermoascus aurantiacus miehe, and thermomyces lanuginosus tsik., obtained from livestock feeds and poultry droppings in nigeria were studied. an optimal ph-temperature range of 5.5-6.5 and 37 degrees - 45 degrees c was recorded for the growth of these fungi. various sources of carbon supplied in the growth medium, except sorbose and rhamnose, were utilized for growth. only aspergillus fumigatus caused consi ...19817023082
studies on the peptide bond specificity and the essential groups of an acid proteinase from aspergillus fumigatus.the specificity and mode of action of an acid proteinase from a. fumigatus was studied with b-chain of insulin, angiotensin ii and bradykinin. with reference to the known structure of the b-chain of insulin and angiotensin ii, the major sites of action were determined. acid proteinase of a. fumigatus hydrolyzed primarily three peptide bonds in the b-chain of insulin viz. i. his(5)-leu(6); ii. tyr(16)-leu(17) and iii. phe(24)-phe(25) bonds. additional cleavages of the bonds his(10)-leu(11) and le ...19817024192
analysis of wall antigens of aspergillus fumigatus by two-dimensional immunoelectrophoresis.water-soluble and surface-located antigens from aspergillus fumigatus mycelium were analysed by two-dimensional electrophoresis (2d iep) with selected sera. a complex pattern was seen when a water-soluble fraction reacted with hyperimmune sera. significantly fewer precipitin peaks were seen when wall-located antigens were used in the 2d iep system; this made possible the detection of a small number of recurring antigen-antibody interactions when sera from patients with aspergilloma or allergic b ...19817007645
allergic bronchopulmonary aspergillosis with low serum immunoglobulin e.elevated total serum ige is helpful in making the diagnosis of abpa and in monitoring the onset of exacerbations and response to therapy. abpa occurs frequently in patients with cf, and in these instances a high total serum ige is seen. this is the first case report of abpa without an elevated total serum ige level. at no time prior to this patient's first episode of abpa, at the time of the illness, or at monthly intervals during follow-up did her total serum ige level exceed 29 iu/ml.19817026647
[a case of hypersensitivity pneumonitis due to aspergillus fumigatus (author's transl)]. 19817026850
[foreign bodies in the chest with aspergillus fumigatus infection of surrounding tissues]. 19817027194
[pulmonary aspergillosis and the characteristics of its course in ankylosing spondylitis complicated by pulmonary fibrosis]. 19817027195
[results of 5-fluorocytosine therapy of aspergillosis and candidiasis]. 19817027197
hypothyroidism with epiphysial dysgenesis. 19816784510
fungal pneumonia (part 4): invasive pulmonary aspergillosis. 19816788456
positive immediate skin tests in cystic fibrosis: a possible role for pseudomonas infection.a review was made of the medical records of 49 patients from whose sputum mucoid pseudomonas aeruginosa had been isolated over a 20-month period. this showed that 31 of 42 had positive immediate skin prick tests to common antigens. 21 had positive reactions to aspergillus fumigatus antigens and 23 had precipitins to a. fumigatus antigen. 36 patients had had frequent courses of antibiotics and airway obstruction was present in 47. these results have prompted the hypothesis that the positive skin ...19816789859
serological diagnosis of pulmonary aspergillosis.methods for the preparation of antigens from clinically isolated cultures of aspergillus were standardized. sera from 25 suspected cases of pulmonary aspergillosis were tested against antigens prepared by us, from 4 strains of a. fumigatus and one strain of a. flavus, using the ouchterlony double diffusion and immunoelectrophoretic techniques. of the 25 sera, 18 reacted positively with antigens of a. fumigatus, one with a. flavus and 2 with both these species. antigens of two non-pathogenic aspe ...19816792543
summary and analysis of the mycology proficiency testing survey results of the college of american pathologists. 1976-1979.the results of the cap mycology proficiency testing survey programs for 1976 through 1979 are summarized and analyzed. there has been a slight, but definite, improvement in participant performance over this four year period and considerable improvement over the performance of 1972. the problems in analyzing participant performance are reviewed. the survey program itself is examined in the light of the stated objectives, and areas for improvement noted.19816792904
association of aspergillus fumigatus with sugar-cane bagasse. iii. immunological studies.in view of the epidemiological significance of aspergillus fumigatus in allergic bronchopulmonary aspergillosis (abpa) and its abundance in sugar-cane bagasse the antigenic properties of 11 strains isolated from bagasse have been studied. three strains isolated from other sources were also included in the study. for comparison sake, a. fumigatus var. ellipticus and a. viridi-mutans were also studied. all the strains of a. fumigatus irrespective of the source of isolation were found to be immunol ...19816460397
a new species of aspergillus fumigatus group and comments upon its synonymy.aspergillus fumigatus fries, acolumnaris rai et al. (1) has been raised to a distinct epithet and four different varieties of a. fumigatus (1--2 & 6) viz. a. fumigatus fries. var. albus rai et al., a. fumigatus fries. var. grisei-brunneus rai and singh, a. fumigatus fries. mut. helvola yuill and a. fumigatus fries. var. sclerotiorum rai et al. which were recognised by earlier workers, have also been merged into a. fumigatus.19817012634
survey of human pathogenic actinomycetes and fungi in soil from rome and other italian areas.as part of a study sponsored by the ministry of health of italy, a research program on pathogenic actinomycetes, keratinophilic and pathogenic fungi in soil was carried out. two hundred soil samples, collected from different areas of the city of rome, calabria, emilia romagna, latium, apulia, sardinia, sicily, tuscany and umbria, were examined by several techniques to detect the widest possible variety of pathogenic actinomycetes and fungi. seven isolates of nocardia asteroides, four of actinoma ...19817012635
fatal childhood pulmonary aspergillosis from contact with pigeons. 19817014070
sequential cold-sensitive mutations in aspergillus fumigatus. ii. analysis by the parasexual cycle.from aspergillus fumigatus i-21 (atcc 32722), which grows at temperatures from 12 to 50 degrees c, three multistep, independently derived, cold-sensitive mutants unable to grow at 37 degrees c or below (cs-37) were obtained by sequential exposure to ethylmethane sulfonate (strain at2) or n-methyl-n'-nitro-n-nitrosoguanidine (at1 and at3). these mutants and on5, a five-step cs-37 mutant, were marked by mutations affecting spore color and nutritional requirements and crossed in four combinations b ...19817016281
sequential cord-sensitive mutations in aspergillus fumigatus. iii. mechanism of cold sensitivity.the mechanism of cold sensitivity of aspergillus fumigatus on5, a 37 degrees c-sensitive mutant derived from a. fumigatus i-21 (atcc 32722) by five sequential mutations, was investigated. the rate of in vivo protein synthesis by on5 was not affected for 2 h following a shift from 45 to 34 degrees c, but the rate of in vivo rna synthesis dropped almost immediately. the rna polymerases 2 h following a shift from 45 to 34 degrees c, but the rate of in vivo rna synthesis dropped almost immediately. ...19817016282
skin tests in a primate model of allergic bronchopulmonary aspergillosis.we have previously reported producing a primate model of allergic bronchopulmonary aspergillosis in which monkey precipitating igg and transfused human ige antibodies against aspergillus fumigatus (af) combined with aerosolized af to produce an inflammatory response in the lung. in this study we attempt to demonstrate similar changes in the skin. immunized and unimmunized monkeys with and without igg precipitating antibody to af were injected at multiple sites intradermally with normal human ser ...19817016762
immunologic studies of disseminated infection with aspergillus fumigatus in the nude mouse.immunity to infection with aspergillus fumigatus conidia was studied in the athymic nude (nu/nu) mouse. the intratracheal, intravenous, and intraperitoneal routes of infection were used. nu/nu mice were not significantly more susceptible than their heterozygous (nu/+) littermates. however, only nu/+ animals could be protected by prior immunization and developed positive, a. fumigatus-specific skin tests. protection could be transferred to nu/nu recipients by immune glass-adherent cells. thus, im ...19817017022
aspergillus endocarditis in children: case report and review of the literature.aspergillus fumigatus endocarditis developed in a 2 1/2-year-old girl after repair of tetralogy of fallot. there have been 14 other cases of aspergillus endocarditis in children described in the literature. fever and embolic phenomenon, particularly to the cns, were the most common presenting manifestations. consumptive coagulopathy developed in this patient as it has in other children and should suggest the diagnosis of aspergillus endocarditis inasmuch as blood cultures are uniformly negative. ...19817017583
a study of immunoglobulin g subclasses in patients with allergic bronchopulmonary aspergillosis. 19817018738
defective aspergillus killing by neutrophil leucocytes in a case of systemic aspergillosis.a persistent defect of aspergillus killing was observed in the neutrophils of a 6-year-old patient with a systemic a. fumigatus infection which was highly refractory to anti-mycotic therapy. aspergillus phagocytosis in vitro was normal, but nearly 80% of the ingested organisms (versus 30% in the controls) survived intracellularly during the 2-hr assay period. the patient's neutrophils showed a subnormal frequency of nitroblue tetrazolium reduction and a subnormal hexose monophosphate shunt activ ...19817018757
physico-chemical properties of the acid proteinase from a. fumigatus.the physico-chemical properties of the purified acid proteinase were investigated. this enzyme was found to hydrolyze hemoglobin maximally among the different substrates used. the maximum hydrolysis of hemoglobin was found at ph 2.8 and 45 degrees c in 30 min. km value of 5.5 x 10(-4) m was obtained from the lineweaver burk plot for the hydrolysis of hemoglobin. the enzyme was found to be most stable at ph 4.9. the maximum stability of the enzyme was observed with 2 per cent casein as a stabilis ...19817019156
further evaluation of indirect immunofluorescence methods for detection of antibodies against aspergillus fumigatus.the if titres of igg antibodies to hyphal antigen preparations of 6 isolates of aspergillus fumigatus were compared with the titres to germling antigen preparations of the same isolates. titres varied according the antigen used and the method of preparation. no single isolate or method of antigen preparation enabled a group of 15 patients with aspergillosis to be separated from a group of asthmatic subjects or a group of patients with other fungal infections, but significantly higher means if ti ...19817013114
enzootic aspergillosis in wild red vented cockatoos in the philippines.philippine red vented cockatoos were being trapped in the wild and dispersed directly to private homes, aviaries, pet stores and for export to the united states of america. except for non-aggressive behaviour and tiring easily the subjects showed clinically nothing peculiar. numerous cases of aspergillosis with no clinical signs prior to death were being reported at the united states air force base, clark field, angeles city, philippines. in all cases stress was a predisposing factor to death; s ...19817012630
immunodiagnosis of invasive pulmonary aspergillosis in rabbits. fungal antigen detected by radioimmunoassay in bronchoalveolar lavage fluid.to improve antemortem diagnosis of invasive pulmonary aspergillosis, we used a radioimmunoassay to detect an aspergillus fumigatus antigen in bronchoalveolar lavage fluid (balf) and evaluated this technique in a model of disseminated aspergillosis. antigen was detected in 91% of balf samples obtained from 11 rabbits with major pulmonary aspergillosis. in an additional 10 rabbits with only minor pulmonary involvement, antigen was detected in 40% of concentrated lavage samples. in contrast, antige ...19817020504
aspergillus pneumonia in an ostrich (struthio camelus). 19817020676
mycotic emboli of the peripheral vessels: analysis of forty-four cases.two cases of mycotic emboli of the peripheral vessels are presented, and 42 additional cases from the literature are analyzed. male patients predominate 3:1. candida and aspergillus are the usual pathogens. initial presentation as large vessel peripheral emboli is characteristic (77%), with emboli originating from either the aortic or mitral valves. cerebral emboli may proceed of follow the peripheral embolization. predisposing factors include open-heart surgery, antibiotic therapy, concomitant ...19817022728
[bronchocentric granulomatosis (author's transl)].a case report and a review of this recently described entity are presented. a feature of interest in our case is the presence of aspergillus fumigatus at the pulmonary needle biopsy as well as in the surgical specimen. this finding is described for the first time in a non asthmatic patient.19816276988
serological cross-reactivity between sporothrix schenckii and various unrelated fungi.the serological cross-reactivity of sporothrix schenckii with various unrelated fungi was investigated by use of immunodiffusion tests. a rabbit anti s. schenckii serum was obtained, which reacted with cladosporium werneckii, c. carrionii, c. bantianum, coccidioides immitis, phialophora jeanselmei, p. gougerotii, p. dermatitidis, fonsecaea pedrosoi, aspergillus fumigatus, histoplasma capsulatum and trichophyton mentagrophytes, but not with saccharomyces cerevisiae antigens. the serological deter ...19816163987
plasma alpha-fetoprotein concentrations in pregnant cows exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus.bovine alpha-fetoprotein (afp) was determined in maternal plasma, using radioimmunoassay in an attempt to detect and monitor fetal distress in pregnant cows. plasma from pregnant cows in the 4th to 5th month of the gestation which had been exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus was used. plasma afp concentrations were determined at intervals from before the cows were exposed until they had aborted or calved. the plasma afp concentration of the exposed pregnan ...19816176152
new, special stain for histopathological diagnosis of cryptococcosis.the masson-fontana silver stain for melanin was employed for the differentiation of pathogenic fungal species in human or mouse tissues. the fungi studied were candida albicans, candida tropicalis, candida glabrata (torulopsis glabrata), cryptococcus neoformans, cryptococcus bacillisporus, coccidioides immitis, blastomyces dermatitidis, histoplasma capsulatum, paracoccidioides brasiliensis, sporothrix schenckii, rhizopus rhizopodiformis, and aspergillus fumigatus. the tissue sections stained wit ...19816162857
immunoperoxidase staining in the recognition of aspergillus infections.this study concerns the use of the indirect immunoperoxidase technique for labelling aspergillus species in infected human and animal tissues in smears taken from cultures. specific labelling without serious cross-reactivity with candida species or zygomycete fungi was demonstrated using antiserum to aspergillus. however, it was not possible to distinguish between two aspergillus species. a fumigatus and a. flavus with this technique. pretreatment of sections with sodium azide and normal swine s ...19816168556
mechanisms of infection in the respiratory tract.related to its potential vulnerability the respiratory tract has a very complex and effective defence apparatus. the interaction between these defence mechanisms and certain characteristics of aetiological agents results in a pattern in which initial infections by these agents tend to occur at specific sites in the tract. infections in which the primary portal of entry is in the upper respiratory tract include bordetella bronchiseptica and haemophilus spp in pigs; pasteurella spp in cattle, shee ...198116030806
damage to aspergillus fumigatus and rhizopus oryzae hyphae by oxidative and nonoxidative microbicidal products of human neutrophils in vitro.our previous studies established that human neutrophils could damage and probably kill hyphae of aspergillus fumigatus and rhizopus oryzae in vitro, primarily by oxygen-dependent mechanisms active at the cell surface. these studies were extended, again quantitating hyphal damage by reduction in uptake of (14)c-labeled uracil or glutamine. neither a. fumigatus nor r. oryzae hyphae were damaged by neutrophils from patients with chronic granulomatous disease, confirming the importance of oxidative ...19826292103
[fungal rhinitis in the dog. diagnosis and therapy]. 19826294907
interactions among amphotericin b, 5-fluorocytosine, ketoconazole, and miconazole against pathogenic fungi in vitro.interactions among amphotericin b, 5-fluorocytosine, ketoconazole, and micoconazole were tested for all possible paired and triple drug combinations and all four drugs combined against three isolates of candida albicans, three candida spp., two isolates of cryptococcus neoformans, and three isolates aspergillus fumigatus. an assay for inhibitory activity was developed in which growth in the presence of an antifungal agent was expressed as a percentage of the growth in drug-free cultures. for nea ...19826295266
combination therapy of experimental candidiasis, cryptococcosis and aspergillosis in mice.combination pairs of the major systematic antimycotic drugs, amphotericin b (amphb), 5-fluorocytosine (5-fc) and ketoconazole (ktz) were administered to mice with experimental candidiasis, cryptococcosis and aspergillosis at a variety of combination ratios. the 3 mycoses were produced with 3 strains each of candida albicans, cryptococcus neoformans, and aspergillus fumigatus, respectively, which were preselected to represent 3 different degrees of 5-fc sensitivity ('normally sensitive', 'moderat ...19826297858
antigenic characterization of some potentially pathogenic mucoraceous fungi.the antigenic profiles of 10 mucoraceous fungi--absidia corymbifera, mortierella wolfii, mucor miehei, m. pusillus, m. racemosus, rhizopus arrhizus, r. microsporus, r. oryzae, r. rhizopodiformis, r. stolonifer,--candida albicans and aspergillus fumigatus were compared by crossed immunoelectrophoresis (xie). antigen-rich material was obtained from homogenized hyphae (or yeasts in the case of c. albicans), and antisera by multiple subcutaneous innoculation of rabbits with macerated but viable hyph ...19826182620
aspergillus osteochondritis after median sternotomy. combined operative treatment and drug therapy with amphotericin b.a case of aspergillus fumigatus osteochondritis after median sternotomy for open heart surgery is presented. to the best of our knowledge, it is the second well documented case that has been reported in the literature. successful healing was obtained with combined operative treatment (consisting of a large resection of the 8th, 9th and 10th right costal cartilages and surrounding soft tissues), and systemic drug therapy with amphotericin b. repeated serologic studies and bone scans were used for ...19826183785
[ketoconazole in the treatment of pulmonary aspergillosis and aspergilloma: mycological observations (author's transl)]. 19826280948
successful treatment of pulmonary and cerebral aspergillosis in an immunosuppressed child.a favourable outcome was observed in a 12 year-old boy who developed invasive pulmonary and cerebral aspergillosis during antineoplastic treatment for central nervous system relapse of acute lymphoblastic leukemia. combination therapy with amphotericin b and 5-fluorocytosine led to complete regression of pulmonary infiltrates. despite enlargement of the cerebral lesion monitored by computerized tomography, no viable fungi were found in the completely resected abscess after a 4 weeks' course of a ...19826749508
[pulmonary aspergillomas in tuberculous caverns]. 19826750707
studies on aspergillus fumigatus from the human lungs.on the basis of cultural, morphological and developmental studies of the two aspergillus isolates from the lungs of an aspergillosis patient, the taxonomic status of aspergillus phialiseptus is discussed. the elimination of the name a. phialiseptus is proposed as the isolates were found by the authors to belong to a. fumigatus.19826750981
extracellular proteolytic activity of aspergillus fumigatus strains with septum-like structures in their phialides in serum-albumin agar--preliminary report. 19826750988
nosocomial aspergillosis: a retrospective review of airborne disease secondary to road construction and contaminated air conditioners.a retrospective review of autopsy records was performed to evaluate a clustering of patients shown to have invasive pulmonary aspergillosis during the period july 1, 1978 through october 31, 1980 at milwaukee county medical center, milwaukee, wisconsin. during this period, ten patients, all of whom were immunosuppressed by either hematologic malignancy (two), advanced age (one), or iatrogenically to prevent rejection of renal allografts (seven) were found to have invasive pulmonary aspergillosis ...19826751070
allergic bronchopulmonary aspergillosis in children. 19826752013
metabolism of progesterone by aspergillus fumigatus. 19826752584
asbestos exposure and aspergillus infection.four cases of asbestos-related changes of the lung complicated by aspergillus infection are described. in such cases, the resulting clinical and radiological picture can give rise to some confusion. it is suggested that exposure to asbestos leads to changes in the bronchi and the lung parenchyma which can facilitate growth of the fungus. it is also possible that asbestos can affect the immune system in such a way that the body's anti-fungal defences are weakened. the aspergillus infection can mi ...19826754414
[comparative study of fungicidal activity in vitro of various quaternary ammonium compounds used in ophthalmology].a study was conducted to evaluate the antifungal activity in vitro of 5 quaternary ammonium compounds. n cetylpyridinium was found to be particularly active against candida albicans and aspergillus fumigatus. the association of n cetylpyridinium and of neosynephrine, found in various eye drop compositions, resulted in potentation of the antifungal activity of this quaternary ammonium.19826754796
aspergillus spinal epidural abscess. 19826754984
[aspergilli and chronic lung diseases].in this study, mycological examinations of morning sputum of patients with chronic bronchitis and bronchial asthma has been performed. specimens were also examined from patients with lobar pneumonia as a control. a fumigatus and a. niger has been isolated from patients chronic bronchitis in frequency of 4.5% and 0.8% respectively. these 2 fungi has also been isolated from patients with bronchial asthma in frequency of 24.5% (a. fumigatus) and 2.4% (a. niger). the frequency of isolation of a. fum ...19826755197
[experimental finding with a new type of fungicide]. 19826755244
enzymic formation of di-d-fructose 1,2'; 2,1' dianhydride from inulobiose by aspergillus fumigatus.the enzyme of aspergillus fumigatus which produces di-d-fructose 1,2'; 2,1' dianhydride (difructose anhydride i) (tanaka et al., 1979) was investigated for its mode of action. fractionation and purification of the enzyme showed that it produced difructose anhydride i not from inulin but from inulobiose by the reverse reaction of the partial hydrolysis of difructose anhydride i.19826757245
laser microbeam studies on fungal tissues. 19826757676
cerebral mass in a leukemic patient. 19826759454
a comparison of surface and cytoplasmic antigens of aspergillus fumigatus in an enzyme-linked immunosorbent assay (elisa). 19826759936
studies on air-borne fungi at qena. iii. thermophilic fungi. 19826759940
[marfan's syndrome complicated by fibrous-cavernous pulmonary tuberculosis and aspergillosis]. 19826760187
saprophytic invasion of infarcted pulmonary tissue by aspergillus species.saprophytic invasion of infarcted pulmonary tissue has received only passing reference in published reports. five patients with colonisation of pulmonary infarcts by aspergillus are described, with discussion of the clinical and histopathological features. the characteristic features were widespread invasion of dead tissue by fungal hyphae, in three cases with liquefaction resulting in the production of a cavitated lesion. there was no evidence that the vascular thrombosis and the consequent inf ...19826760449
[precipitins in sarcoidosis]. 19826763093
[solid-phase radioimmunoassay for measurement of igg, iga and igm antibodies against aspergillus fumigatus]. 19826763647
[mycoses caused by fungal spores in indoor air].fungal spores in indoor air may cause mycoses and allergies in man. the following different types of exposure are interesting from the epidemiological view-point: (a) the occupational exposure (work done with fungi-contaminated material), (b) the accidental exposure (formation and growing of fungi by constructive defects), and (c) the inconspicuous constant exposure (as part of lifestyle, e.g. by plants or small pets). it is stressed that the only way to characterize the indoor air quality with ...19826764028
serodiagnostic value of culture filtrate antigens from aspergilli with septate phialides.culture filtrate antigens from 6 typical isolates of aspergillus fumigatus, 6 isolates with septate phialides, and isolates of a. fumigatus var. ellipticus, a. fishceri, a. flavus and a. niger were studied in tests with 2,100 samples of serum originating from 1,452 patients suffering from miscellaneous diseases, including 60 cases of aspergillosis, 850 samples of serum from apparently healthy blood donors and 522 sera referred to us as positive for c-reactive protein. the culture filtrate antige ...19826801788
xenon-133 evidence of bronchopleural fistula healing during treatment of mixed aspergillus and tuberculous empyema. 19826809909
comparison of aspergillus fumigatus and aspergillus umbrosus antigens in serological tests of farmer's lung.a comparison was made between aspergillus fumigatus and a. umbrosus antigens regarding their usefulness in serological tests of farmer's lung. mycelial and culture fluid antigens of both microbes were used to test sera of 30 finnish farmer's lung patients for antibodies in the elisa and in the gel precipitation (gp) test. antibodies to a. umbrosus were more common than to a. fumigatus; this was especially evident in elisa, elisa gave more positive results with all four antigens than gp. mycelial ...19826812448
rapid enzyme-linked immunosorbent assay (elisa) for aspergillus fumigatus antibodies.a rapid enzyme-linked immunosorbent assay (elisa) where component incubation periods were shortened to one hour, was compared with agar gel double diffusion (agdd) and a standard elisa procedure for detecting antibodies to aspergillus fumigatus in 28 asthmatic patients with suspected allergic aspergillosis. using two a fumigatus antigens the rapid elisa compared well with agdd and the standard elisa method. eleven sera that reacted with both antigens in agdd were all positive against antigen 1 i ...19826813358
serology of aspergillus granuloma.a total of 21 cases of granuloma caused by aspergillus species were encountered during the period 1972-79. the organs involved were nasal and paranasal sinuses, brain, orbit, subcutaneous tissue of check, lungs and endocardial valve in the decreasing order of their frequency. aspergillus flavus was the main etiological agent. immunodiffusion tests with various aspergillus species as antigen showed a positively of 17 out of 18 (94.4 per cent) cases. the role of precipitins in diagnosis and progno ...19826813743
serum antibodies to aspergillus fumigatus in patients with pulmonary aspergillosis detected by immunofluorescence.aspergillus antibodies of the igg, iga and igm class were determined by the indirect immunofluorescence (iif) technique in sera from 11 patients with proven pulmonary aspergillosis (group i), 11 patients with suspected pulmonary aspergillosis (group ii), 34 patients with chronic obstructive lung disease (group iii), 29 patients with lung infiltrates of non-fungal aetiology (group iv), and 94 blood donors (group v). the antigen was cryostate sections of formaldehyde fixed mycelium. igg antibody t ...19826814182
rapid serological diagnosis of aspergillus fumigatus infection of the frontal sinuses and nasal chambers of the dog.aspergillus fumigatus precipitins in dog sera were detected by counterimmunoelectrophoresis (cie). the procedure gave results within 90 minutes compared with 72 to 96 hours required in agar-gel double diffusion. culture-filtrate antigens from two-week-old cultures of a fumigatus and two-day-old mycelial antigens produced the strongest reactions in cie tests and the former antigen also revealed high titres in tests with serum from seven of 14 dogs with a fumigatus precipitins. serum from these 14 ...19826815743
extracellularly proteolysing aspergillus fumigatus and its interaction with specific immune serum in the serum-albumin agar. a preliminary report.serum-albumin agar with the initial ph values of 5.0 or 7.0 allows diffusion and precipitation of a. fumigatus antigens (culture filtrate) and antibodies. in the zone of extracellular proteolysis by a living culture of an atypical a. fumigatus strain, precipitin bands only against a specific immune serum were found in this medium with an initial ph of 7.0. how far under such complex substrate conditions including active extracellular proteolysis, an immune reaction can take place is open to disc ...19826819740
aspergillosis of the nose and paranasal sinuses.aspergillosis is becoming an increasingly recognized pathogen in the sinonasal tract. the courses of two patients, one seemingly healthy and one with a terminal malignancy, are reviewed. these patients illustrate the clinical course, difficulties in diagnosis, and management of patients with aspergillosis of the sinonasal tract. aspergillus is a common endogenous contaminant of the upper respiratory tract; however, bacterial sinusitis may trigger its growth and proliferation. aspergillus fumigat ...19826953293
aspergillosis mycetoma of the maxillary antrum.aspergillus is a fungus, usually found growing as one of the many moulds of fruit, foods and nuts. this paper deals with a ball of fungus growing in the maxillary antrum of an elderly female patient presenting as a radio-opaque expansile mass. in man, aspergillus fumigatus and aspergillus flavis have both been incriminated as pathogenic species in the para nasal sinuses. spores of aspergilli become mixed with dust and can enter the ear, nose and lungs. if conditions are favourable, growth will e ...19826961938
echocardiography in aspergillus endocarditis. 19827036080
oxiconazole, a new imidazole derivative. evaluation of antifungal activity in vitro and in vivo.the new imidazole derivative z-[2,4-dichloro-2-imidazol-1-yl)acetophenone]-o-(2,4-dichlorobenzyl)-oxime nitrate (oxiconazole, ro 13-8996) is characterized by a broad fungistatic spectrum against the agents of human mycoses in vitro. in addition, fungicidal activity of various degree was found in selected species (aspergillus fumigatus, cryptococcus neoformans, candida albicans and trichophyton mentagrophytes). synthesis of dna was inhibited by subinhibitory concentrations of oxiconazole in paral ...19827037014
comparative ocular pathogenicity of cryptococcus neoformans, candida glabrata, and aspergillus fumigatus in the rabbit.in a previous study, 88% of rabbits with disseminated infection caused by candida albicans developed ophthalmoscopically visible, hematogenous endophthalmitis (chorioretinitis) over a 2 week period. to determine the incidence of this ocular complication in disseminated infection caused by cryptococcus neoformans, candida glabrata, and aspergillus fumigatus compared with that caused by c. albicans, the first three species of fungi were injected intravenously (between 10(5) and 10(9) organisms per ...19827037676
selective protection against conidia by mononuclear and against mycelia by polymorphonuclear phagocytes in resistance to aspergillus. observations on these two lines of defense in vivo and in vitro with human and mouse phagocytes.by comparing natural immunity to aspergillus fumigatus (af) in vivo with the action of human or mouse phagocytes against af in vitro, we delineated two sequential lines of defense against af. the first line of defense was formed by macrophages and directed against spores. macrophages prevented germination and killed spores in vitro and rapidly eradicated conidia in vivo, even in neutropenic and athymic mice. the second was the neutrophilic granulocyte (pmn), which protected against the hyphal fo ...19827037853
application of enzyme-linked immunosorbent assay (elisa) in diagnosis of allergic bronchopulmonary aspergillosis.ige and igg antibodies against af were measured by elisa in sera from 15 patients with abpa and compared with antibody levels in six patients with extrinsic asthma and immediate-type skin reactivity to af (prick skin test 3+ or 4+) but no other evidence for abpa. the assay used polyvinyl microhemagglutination plates as a solid phase to adsorb antigens of af and a double-antibody system of class-specific anti-human globulin and alkaline phosphatase-conjugated goat anti-rabbit globulin. ige-af and ...19827038011
an indirect immunofluorescence study of antibodies to aspergillus funigatus in sera from children and adults without aspergillosis.an indirect immunofluorescence (if) method for detection of antibodies of aspergillus fumigatus was developed: it used cryosections of formaldehyde-treated mycelium as antigen. mycelial age was found to be of importance for titer determination, and growth in czapek-dox broth for 48 h at 37 degrees c was considered optimal. fluorescein-conjugated rabbit antisera to human immunoglobulins reacted by direct if with a. fumigatus but this staining could be abolished by absorption of conjugates with ho ...19827038927
hydrolysis of vegetable oils and triglycerides by thermotolerant and zoopathogenic species of aspergillus from nigerian palm produce.the ability of aspergillus fumigatus fres. and aspergillus nidulans (eidam) wint obtained from nigerian palm produce to degrade vegetable oils and triglycerides and the production and activity of their extracellular lipases were studied. both species readily hydrolysed palm oil and palm kernel oil among others liberating free fatty acids in the process. good growth with mycelia production of both fungi were also recorded on the triglycerides used as sources of carbon at 37 degrees c with the bes ...19827040975
isolation of 80s ribosomes from spores of aspergillus fumigatus and their antigenicity in rabbits.ribosomes were obtained from spores of aspergillus fumigatus by mechanical disruption and differential centrifugation. the initial preparation (crude ribosomes) contained spore components which appeared to be broken fragments of plasmalemma with or without organelles. purified ribosomes free of membranous material were prepared by gel filtration chromatography on sepharose cl-4b. monomeric 80s ribosomes consisting of 40% protein and 60% rna, and morphologically characteristic of fungal ribosomes ...19827040976
multiple mycotic aneurysms and transverse myelopathy complicating repair of aortic coarctation.the case of an 18-year-old man with coarctation of the aorta discovered on routine physical examination and subsequently surgically repaired is reported. four months postoperatively, aneurysms developed at the repair site and thrombosis of both femoral arteries was noted. following an attempt to repair the aneurysm and remove the thrombi, the patient became paraplegic; aspergillus fumigatus was found infecting the aorta and femoral vessels. after additional operations and a course of amphoterici ...19827039535
reproducibility of prick skin tests to five allergens.thirty-five patients had prick skin tests to the common allergens candida albicans. aspergillus fumigatus, grass pollen, horse dander and dermatophagoides pteronyssinus performed on a regular basis from autumn 1973 to autumn 1975. specific ige to the same allergens (except c. albicans) was determined at the time of skin testing for the first five seasons. it was found that the position on the volar aspect of the forearm on which the test was performed did not affect the reaction. there was a sig ...19827039862
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