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alteration in certain enzymological parameters of an indian major carp, cirrhinus mrigala exposed to short- and long-term exposure of clofibric acid and diclofenac.the extensive use of pharmaceuticals in human and veterinary medicine may enter the aquatic environment and pose a serious threat to non-target aquatic organisms like fish. in this study, indian major carp cirrhinus mrigala was exposed to different concentrations (1, 10 and 100 μg l⁻¹) of most commonly used pharmaceutical drugs clofibric acid (ca) and diclofenac (dcf) to evaluate its impacts on certain enzymological parameters during short- and long-term exposures. during short-term (96 h) expos ...201323579460
characterization and physical mapping of 18s and 5s ribosomal genes in indian major carps (pisces, cyprinidae).characterization of the major (18s) and minor (5s) ribosomal rna genes were carried out in three commercially important indian major carp (imc) species, viz. catla catla, labeo rohita and cirrhinus mrigala along with their physical localizations using dual colour fluorescence in situ hybridization. the diploid chromosome number in the above carps was confirmed to be 50 with inter-species karyo-morphological variations. the 18s rdna signals were observed on 3 pair of chromosomes in c. catla and l ...201323587674
visco-elastic and flow properties of gelatin from the bone of freshwater fish (cirrhinus mrigala).the average yield of gelatin from the bone of freshwater fish (cirrhinus mrigala) was 6.13%. the fluorescence spectra revealed maximum emission at 303 nm indicating the exposure of chromophores to bulk solvent. the amino acid profile of gelatin revealed a higher proportion of glycine and imino acids. the bloom strength of gelled gelatin was 159.8 g. the average molecular weight of fish bone gelatin was 281 kda as determined by gel filtration technique. the dynamic oscillatory test of gelatin sol ...201323701696
review of the bioenvironmental methods for malaria control with special reference to the use of larvivorous fishes and composite fish culture in central gujarat, india.mosquito control with the use of insecticides is faced with the challenges of insecticide resistance in disease vectors, community refusal, their high cost, operational difficulties, and environmental concern. in view of this, integrated vector control strategies with the use of larvivorous fishes such as guppy (poecilia reticulata) and gambusia (g. affinis) as biological control agents were used in controlling mosquito breeding in different types of breeding places such as intradomestic contain ...201323703433
short and long-term effects of clofibric acid and diclofenac on certain biochemical and ionoregulatory responses in an indian major carp, cirrhinus mrigala.extensive use of pharmaceuticals in human and veterinary medicine and aquaculture practices pose a serious threat to aquatic organisms. in the present investigation, cirrhinus mrigala an indian major carp was exposed to different concentrations of clofibric acid (ca) and diclofenac (dcf) and certain biochemical and ionoregulatory responses were assessed under short and long term exposures. during short-term (96h) exposure period, plasma glucose and sodium (na(+)) levels were increased at all con ...201323777676
population divergence and structure of cirrhinus mrigala from peninsular rivers of india, revealed by mitochondrial cytochrome b gene and truss morphometric analysis.genetic diversity and population structure of cirrhinus mrigala from peninsular riverine systems of india was studied using mitochondrial dna gene, cytochrome b and truss morphometric analysis. analysis of 982 bp of the cytochrome b gene from 182 samples collected from six rivers revealed the presence of 28 haplotypes with overall high haplotype diversity value of 0.78981 and nucleotide diversity value of 0.00215. analysis of molecular variance (amova) showed that genetic variation is mainly har ...201423795828
heavy metal accumulation in edible fish species from rawal lake reservoir, pakistan.the aim of the present study was to describe the accumulation of trace metals in the liver, kidney, gills, muscles, and skin of four edible fish species (tor putitora, cirrhinus mrigala, labeo calbasu, and channa punctatus) of rawal lake reservoir, pakistan. the fish samples were collected in the pre-monsoon (may 2008) and post-monsoon (october 2007) seasons and were analyzed for heavy metals by using an atomic absorption spectrometer. kidney and liver showed relatively high concentrations of he ...201423884877
characterization and expression analysis of common carp cyprinus carpio tlr5m.tlr5 is responsible for the recognition of bacterial flagellin in vertebrates. in this study, we cloned the tlr5m gene of common carp using the rapid amplification of cdna ends (race) method. the tlr5m cdna was 3182 bp in length and contained a 2658-bp open reading frame, which encoded a protein of 885 amino acids (aa). the entire coding region of the tlr5m gene was successfully amplified from genomic dna and contained a single exon. the aa sequence of carp tlr5m showed the highest similarity (8 ...201323930591
nod1 and nod2 receptors in mrigal (cirrhinus mrigala): inductive expression and downstream signalling in ligand stimulation and bacterial infections.nucleotide binding and oligomerization domain (nod)1 and nod2 are important cytoplasmic pattern recognition receptors (prrs) and key members of the nod-like receptor (nlr) family. they sense a wide range of bacteria or their products and play a key role in inducing innate immunity. this report describes the role of nod1 and nod2 receptors signalling in innate immunity in the indian major carp, mrigal (cirrhinus mrigala). tissue-specific expression analysis of nod1 and nod2 genes by quantitative ...201323938386
a comparative study of coi and 16 s rrna genes for dna barcoding of cultivable carps in india.the 5' region of the mitochondrial dna gene cytochrome c oxidase subunit i (coi) is the standard marker for dna barcoding. however, 16 s rrna has also been advocated for dna barcoding in many animal species. herein, we directly compare the usefulness of coi and 16 s rrna in discriminating six cultivable carp species: labeo rohita, catla catla, cirrhinus mrigala, labeo fimbriatus, labeo bata and cirrhinus reba from india. analysis of partial sequences of these two gene fragments from 171 individu ...201524047160
characterization of carboxylesterase in skin mucus of cirrhinus mrigala and its assessment as biomarker of organophosphate exposure.presence of carboxylesterase (cbe) activity in the skin mucus of cirrhinus mrigala was investigated. cbe activity in skin mucus showed higher substrate preference for α-naphthyl acetate over p-nitrophenyl acetate. four cbe isozymes-cbe-1, cbe-2, cbe-3, and cbe-4 were observed in skin mucus during zymography. the isozyme cbe-4 was characterized as typical serine esterase, whereas cbe-1, cbe-2, and cbe-3 were identified as sulphhydryl group-dependent serine esterases. in vitro treatment of skin mu ...201424092332
histological and histochemical investigations of the pharyngeal jaw apparatus of a carp cirrhinus mrigala.the histological organization and histochemical aspects of the pharyngeal jaw apparatus of the indian major carp cirrhinus mrigala are described. the pharyngeal jaw apparatus consists of a lower pharyngeal jaw and a chewing pad. histochemical analysis reveals that the epithelium covering the lower pharyngeal jaw is mucogenic and is involved in the secretion of different classes of glycoproteins (gps) including gps with oxidizable vicinal diols, gps with o-sulphate esters and gps with sialic acid ...201424103525
impact of exotic carps in the polyculture with indigenous carps: competition for food.the fingerlings of indigenous carps such as catla (catla catla), rohu (labeo rohita) and mrigal (cirrhinus mrigala) with exotic carps such as silver carp (hypophthalmichthys molitrix), bighead carp (aristichthys nobilis) and mirror carp (cyprinus carpio) were cultured together in a fish pond at bangladesh agricultural university, mymensingh, in order to determine the food electivity, dietary overlap and food competition among indigenous major carps and exotic carps. phytoplankton (chlorophyceae, ...201224191618
relativein vitro effectiveness of estradiol-17β, androgens, corticosteroids, progesterone and other pregnene derivatives on germinal vesicle breakdown in oocytes of indian major carps,labeo rohita, cirrhinus mrigala andcatla catla.the relative effectiveness of estradiol-17β, androgens, corticosteroids, progesterone and other pregnene derivatives on germinal vesicle breakdown (gvbd) was investigatedin vitro using folliculated oocytes of three carps,labeo rohita, cirrhinus mrigala, andcatla catla. in all three species progesterone and 17α-hydroxyprogesterone could induce gvbd but relatively 17α,20β-dihydroxyprogesterone was consistently found to be the most potent maturation-inducing steroid. both estradiol-17β and testoste ...198924221598
chemical composition and functional properties of mrigal (cirrhinus mrigala) egg protein concentrates and their application in pasta.protein concentrates were prepared from underutilized mrigal (cirrhinus mrigala) roe to produce value added by-products for food applications. chemical composition and physicochemical properties of protein concentrates prepared from mrigal (cirrhinus mrigala) roes were determined. the effects of ph and salt concentration on protein solubility were investigated. the protein content of the concentrates was found to be 75, and 91%, respectively for dehydrated and defatted powders. the maximum prote ...201324425946
post-harvest loss of farm raised indian and chinese major carps in the distribution channel from mymensingh to rangpur of bangladesh.post-harvest loss of catla (catla catla), rohu (labeo rohita), mrigal (cirrhinus mrigala), silver carp (hypophthalmichthys molitrix), grass carp (ctenopharyngodon idella) and sarpunti (puntius sarana) in a single distribution chain from harvest in mymensingh to retail sale rangpur town were determined, in order to obtain information on quality deterioration and existing handling and icing conditions so that suggestions for improving such practices can be made. quality defect points of the fish a ...201324494525
comparative organoleptic quality of indian major carps collected from pond and reservoir.an attempt was made to distinguish the possible organoleptic differences between the pond reared and wild fish species of reservoirs and correlate these with the physicochemical characteristics of water bodies. the texture was found to firmer in wild varieties of fishes than pond reared fish, with best in catla catla followed by labeo rohita and cirrhinus mrigala. all the cultured fishes had lighter colour than wild fish species which were darker in appearance. comparatively, higher dissolved ox ...201324640254
effect of dietary supplementation of l-tryptophan on thermal tolerance and oxygen consumption rate in cirrhinus mrigala fingerlings under varied stocking density.a 60 day feeding trial was conducted to study the effect of dietary l-tryptophan on thermal tolerance and oxygen consumption rate of freshwater fish, mrigala, cirrhinus mrigala reared under ambient temperature at low and high stocking density. four hundred eighty fingerlings were distributed into eight experimental groups. four groups each of low density group (10 fishes/75l water) and higher density group (30 fishes/75l water) were fed a diet containing 0, 0.68, 1.36 or 2.72% l-tryptophan in th ...201424679973
effect of ice storage on the functional properties of proteins from a few species of fresh water fish (indian major carps) with special emphasis on gel forming ability.in the present study the effect of ice storage on physico-chemical and functional properties of proteins from indian major carps with special emphasis on gel forming ability have been assessed for a period of 22 days. the solubility profile of proteins in high ionic strength buffer and calcium adenosine triphosphatase (atpase) enzyme activity reduced significantly (p < 0.05), while that of total volatile base nitrogen (tvb-n) increased significantly (p < 0.05) at the end of 22 days of ice storag ...201424741158
cross-priming of microsatellite loci in subfamily cyprininae (family cyprinidae): their utility in finding markers for population genetic analysis in three indian major carps.this study is aimed to identify polymorphic microsatellite markers and establish their potential for population genetics studies in three carp (family cyprinidae; subfamily cyprininae) species, labeo rohita, catla catla and cirrhinus mrigala through use of cyprinid primers. these species have high commercial value and knowledge of genetic variation is important for management of farmed and wild populations. we tested 108 microsatellite primers from 11 species belonging to three different cyprini ...201424792330
a new growth curve model for biological growth: some inferential studies on the growth of cirrhinus mrigala.growth of living organisms is a fundamental biological process. it depicts the physiological development of the species related to the environment. mathematical development of growth curve models has a long history since its birth. we propose a mathematical model to describe the evolution of relative growth rate as a function of time based on a real life experiment on a major indian carp cirrhinus mrigala. we establish that the proposed model is able to describe the fish growth dynamics more acc ...201424933474
the complete mitochondrial genome sequence of cirrhinus mrigala (♀) × labeo rohita (♂).we cloned and sequenced the complete mitochondrial dna (mtdna) of cirrhinus mrigala (♀) × labeo rohita (♂) by pcr-based method. the total of the mtdna was 16,595 bp. it consisted of 13 protein-coding genes, 22 trnas, 2 rrna genes, and one putative control region (d-loop region). the overall base composition on strand was as follows a: 32.09%, g: 15.51%, c: 27.95%, t: 24.45%, and the a + t content 56.54%. all the protein-coding genes initiated by typical atg codon, and ten genes ended with the co ...201625350736
toxicological effects of clofibric acid and diclofenac on plasma thyroid hormones of an indian major carp, cirrhinus mrigala during short and long-term exposures.in the present investigation, the toxicity of most commonly detected pharmaceuticals in the aquatic environment namely clofibric acid (ca) and diclofenac (dcf) was investigated in an indian major carp cirrhinus mrigala. fingerlings of c. mrigala were exposed to different concentrations (1, 10 and 100μgl(-1)) of ca and dcf for a period of 96h (short term) and 35 days (long term). the toxic effects of ca and dcf on thyroid hormones (ths) such as thyroid stimulating hormone (tsh), thyroxine (t4) an ...201425461555
trypsin from the digestive system of carp cirrhinus mrigala: purification, characterization and its potential application.trypsin was purified 35.64-fold with 4.97% recovery from the viscera of carp cirrhinus mrigala (mrigal) by ammonium sulfate precipitation, ion exchange and affinity chromatography. the purified enzyme was active at a wide range of ph (7.0-9.2) and temperature (10-50°c). the purified enzyme exhibited high thermal stability up to 50°c for 1h. the enzyme activity was stabilized by ca(+2) (2mm) up to 7h at 40°c. the km and kcat values of purified enzyme were 0.0672 mm and 92.09/s/mm, respectively. s ...201525577096
susceptibility, metacercarial burden, and mortality of juvenile silver barb, common carp, mrigal, and tilapia following exposure to haplorchis taichui.fish-borne zoonotic trematodes (fzt) including heterophyids and opisthorchiids are prevalent in native and aquaculture fish in southeast asia. fzt are reported highly prevalent in juvenile aquaculture fish that belonged to the family heterophyidae, particularly haplorchis taichui. aquaculture fish are reported to have varying levels of natural infection with h. taichui, but data for their susceptibility as well as resistance to infection are not available. the aim of this study was to determine ...201525636245
isolation and expression of grass carp toll-like receptor 5a (citlr5a) and 5b (citlr5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection.toll-like receptor 5 (tlr5), a member of toll-like receptors (tlrs) family and is responsible for the bacterial flagellin recognition in vertebrates, play an important role in innate immunity. in the study, two tlr5 genes of grass carp (ctenopharyngodon idellus), named citlr5a and citlr5b, were cloned and analyzed. both citlr5a and citlr5b are typical tlr proteins, including lrr motif, transmembrane region and tir domain. the full-length cdna of citlr5a is 3054 bp long, with a 2646 bp open readin ...201525665802
effect of temperature on the prevalence of different parasites in cirrhinus mrigala hamilton of west bengal.a study has been done to find out the prevalence of different kinds of parasites in cirrhinus mrigala (hamilton 1822) during 2010-2011. it has been found that the temperature variation affects some parasitic infestation over the fish species. it was found that ciliophoran and crustacean parasites are more prevalent from november to february whereas the myxozoan and monogenean parasites are more prevalent from january to april. considering temperature variation throughout the year it has been inf ...201525698872
[studying the effect of heavy metals on tissue protein of an edible fish cirrhinus mrigala under the influence of ph and water hardness].the goal of the study was to investigate the influence of ph and water hardness on protein contents of muscle tissues of cirrhinus mrigala fingerlings by fourier transform infrared spectroscopy. ft-ir spectra revealed significant differences in absorbance intensities between control and toxic metal-treated muscle tissues, reflecting a change in protein contents due to heavy metals, nickel and chromium, influence at different ph and water hardness. metal toxicity is also more pronounced at ph 9.0 ...201525702493
genetic differentiation in indian major carp, cirrhinus mrigala (hamilton, 1822) from indian rivers, as revealed by direct sequencing analysis of mitochondrial cytochrome b region.a 307 bp segment of cytochrome b gene of mtdna was sequenced and analyzed for 90 individuals of cirrhinus mrigala collected across the three rivers, namely ganges, narmada and brahmaputra. analyses revealed the presence of 14 haplotypes with haplotype diversity (h) ranging from 0.304 to 0.692, and nucleotide diversity (π) 0.002-0.043. the majority of variation was found within the population (96.21%), and the fst value (0.035) as well as the value of exact test of population differentiation (0.8 ...201525909754
barcoding of fresh water fishes from pakistan.dna bar-coding is a taxonomic method that uses small genetic markers in organisms' mitochondrial dna (mt dna) for identification of particular species. it uses sequence diversity in a 658-base pair fragment near the 5' end of the mitochondrial cytochrome c oxidase subunit 1 (co1) gene as a tool for species identification. dna barcoding is more accurate and reliable method as compared with the morphological identification. it is equally useful in juveniles as well as adult stages of fishes. the p ...201625980661
isolation and characterization of collagen from fish waste material- skin, scales and fins of catla catla and cirrhinus mrigala.the collagen of skin, scales and fins of catla catla and cirrhinus mrigala were isolated and characterised. nine fishes of each fish species of three weight groups were collected from a commercial fish farm. collagen characterisation using sds-page revealed the molecular weights (kda) of the c. catla skin, scales, and fins which ranged from 120 to 210, 70 to 201, and 68 to 137 kda, respectively. the size of the collagen of c. mrigala skin, scales and fins ranged from 114 to 201, 77 to 210, and 7 ...201526139894
morphological and molecular characterization of henneguya bicaudi n. sp. (myxosporea: myxobolidae) infecting gills of cirrhinus mrigala (ham.) in harike wetland, punjab (india).in the present study, henneguya bicaudi n. sp. infecting gills of cirrhinus mrigala (ham.) an indian major carp from harike wetland in punjab, india, has been described on the basis of myxospore morphology and partial amplification of the ssu rrna gene. the pseudocysts of h. bicaudi n. sp. were located within the gill filament, whitish with round to irregular outer margin and 0.5-0.7 mm in diameter, 3-4 pseudocysts each containing about 50-100 myxospores were detected per gill. mature myxospores ...201526239799
occurrence, bioaccumulation and risk assessment of dioxin-like pcbs along the chenab river, pakistan.this study aimed to assess the occurrence, distribution and dietary risks of seven dl-pcbs (dioxin-like pcbs) in eleven collected fish species from chenab river, pakistan. ∑7dl-pcbs (ng g(-1), wet weight) burden was species-specific and the maximum average concentrations were found in mastacembelus armatus (5.43), and rita rita (5.1). correlation of each dl-pcbs with δ(15)n%, indicated a food chain accumulation process of these chemicals into chenab river, pakistan. species-specific toxicity of ...201526342456
description of a new species of myxobolus butschli, 1882 from the cirrhinus mrigala hamilton, 1822 an edible fish of india.myxobolus bütschli 1882 is an important group of parasitic protozoa infecting a large number of freshwater fish species worldwide. the severity of myxobolus infection leads to heavy loss in production of fishes. during the survey of the fish parasites, a new species of the genus myxobolus have been isolated from the gills of a freshwater edible fish cirrhinus mrigala hamilton, 1822 from canning, south 24 parganas, west bengal, india which has been described here.201526345052
expression of mx gene in cirrhinus mrigala (hamilton, 1822) to ompc protein of aeromonas hydrophila and bacterial infection.the aims of this study were to identify alternative myxovirus (mx) stimulatory compounds in cirrhinus mrigala and to characterize the kinetics and intensity of their stimulated responses by semi-quantitative rt-pcr. mx transcripts were measured in c. mrigala injected with aeromonas ompc (outer membrane protein) at a dose 0.4 mg/fish. at day 1, day 2, day 3, day 5, day 10, day 20 and day 30, samples were collected from kidney, spleen, liver, heart brain, gill, intestine and muscle for the study o ...201626487188
prevalence, site and tissue preference of myxozoan parasites infecting gills of cultured fish in punjab (india).native carp species cultured in indian farms in punjab (catla catla catla, rohu labeo rohita, mrigal cirrhinus mrigala, exotic carps such as silver carp hypophthalmichthys molitrix, grass carp ctenopharyngodon idella, common carp cyprinus carpio and a catfish sperata seenghala) were examined for the presence of myxozoan parasites infecting gills. firstly, the gills were examined under a zoom-stereomicroscope for the presence of plasmodia. the number of plasmodia per gill was counted to determine ...201626912043
angiotensin i-converting enzyme (ace) inhibitory activity and structural properties of oven- and freeze-dried protein hydrolysate from fresh water fish (cirrhinus mrigala).the angiotensin i-converting enzyme (ace) inhibitory activity and structural properties of oven-dried (od-fph) and freeze-dried (fd-fph) protein hydrolysates derived from fresh water fish (cirrhinus mrigala) muscle, using papain, were investigated. amino acid profiles indicated a higher proportion of hydrophobic residues in od-fph and hydrophilic residues in fd-fph samples. fourier transform infrared (ft-ir) spectra revealed random coil structure in od-fph and β-sheet in fd-fph samples. the appr ...201627041318
variation in genotoxic susceptibility and biomarker responses in cirrhinus mrigala and catla catla from different ecological niches of the chenab river.a large number of methods have been applied to evaluate genotoxic damage in different aquatic species. comet assay, as a method for detecting dna alterations, and micronucleus test, as an index of chromosomal damage are the most widely used and authentic methods in laboratory and field studies. the primary objective of the study was to evaluate the genotoxic effects of heavy metals generated by toxic industrial effluents and various kinds of pollutants from urban and agricultural areas and domes ...201627068917
variation in susceptibility pattern of fish to argulus siamensis: do immune responses of host play a role?branchiuran ectoparasites of the genus argulus can have extensive damaging effects on cultured fish. there exist no systematic studies that evaluate susceptibility or resistance of various carp species to argulus sp. and the underlying mechanisms. the present study aimed at identifying the most susceptible and resistant cultured species, studying settlement and survival of parasite on these species, and finally unravelling the variations of immune response in both resistant and susceptible speci ...201627084477
first report on fish cysteine as a biomarker of contamination in the river chenab, pakistan.the eastern and southern parts of the faisalabad city produce considerable quantities of industrial and municipal pollutants, much of which is drained into the river chenab, reducing the productivity of fauna and flora in the river. this study was aimed to determine whether cysteine is useful as a biomarker of exposure to polluted fresh water. the amino acid profile of fish muscle was analyzed by paper chromatography in cirrhinus mrigala and labeo rohita from the river chenab to determine habita ...201627117257
effect of oven drying and freeze drying on the antioxidant and functional properties of protein hydrolysates derived from freshwater fish (cirrhinus mrigala) using papain enzyme.fish protein hydrolysate (fph) was prepared from fresh water fish cirrhinus mrigala using papain and dried in oven (od-fph) and freeze dryer (fd-fph). the electron micrographs of fd-fph samples showed porous structure. the browning intensity of od-fph samples was higher than the fd-fph samples. the dpph (2, 2 diphenyl-1-picrylhydrazyl) free radical scavenging activity and linoleic acid peroxidation inhibition activity of fph were not affected by oven drying process. the sequential digestion of f ...201627162411
probiotic potential of autochthonous bacteria isolated from the gastrointestinal tract of four freshwater teleosts.in this study, a total of 121 bacterial strains were isolated from the gastrointestinal tract of four teleostean species, namely striped snakehead (channa striatus), striped dwarf catfish (mystus vittatus), orangefin labeo (labeo calbasu) and mrigal carp (cirrhinus mrigala), among which 8 isolates showed promising antibacterial activity against four potential fish pathogens, aeromonas hydrophila, aeromonas salmonicida, aeromonas sobria and pseudomonas fluorescens and were non-hemolytic. the isol ...201727557836
potential probiotics from indian major carp, cirrhinus mrigala. characterization, pathogen inhibitory activity, partial characterization of bacteriocin and production of exoenzymes.the study explored antagonistic activity of the cellular components of potential probiotic bacteria from mrigal (cirrhinus mrigala) against fish pathogens with a basic insight of the chemical nature of the antagonistic compound. totally 208 autochthonous gut bacteria were isolated, of which 22 strains revealed antagonism towards ≥2 of the six common fish pathogens. zones of inhibition (halo diameter) were presented as score and the four most promising strains were selected as putative probiotics ...201627663374
fish scales as a non-lethal tool of the toxicity of wastewater from the river chenab.water pollution is gradually increasing in natural waters through anthropogenic activities. this study aimed to use fish scales as a bio-indicator of pollution, along with water quality parameters, and the assessment and detection of selected heavy metals in water samples collected from the river chenab, including the chakbandi drain that gathers domestic sewage waste and industrial effluents from faisalabad and deposits it into this freshwater body. all water quality parameters (ph, total disso ...201727817145
molecular characterization of myxobolus cuttacki (myxozoa, myxosporea, bivalvulida) infecting gill lamellae of minor carp labeo bata (ham.).as new pathogenic strains are emerging and threatening aquaculture development, myxosporeans (myxozoa) are receiving much attention in recent years. myxosporean taxonomy is traditionally based on morphology of the myxospore stage. molecular data on indian myxosporeans are rare. in this report, the 18s rrna gene sequence of myxobolus cuttacki infecting gill lamellae of minor carp labeo bata (ham.) and its phylogenetic relationship with other myxobolids are described for the first time. the plasmo ...201427843987
molecular phylogeny of myxobolus orissae (myxosporea: myxobolidae) infecting the gill lamellae of mrigal carp cirrhinus mrigala (actinopterygii: cyprinidae).myxosporeans are best known for the diseases they cause in commercially important fish species. identification of myxosporeans at the species-level is mainly based on conventional methods. the 18s rrna gene sequence of morphologically identified myxobolus orissae infecting the gill lamellae of mrigal carp cirrhinus mrigala was characterized in the present study. the plasmodia of m.orissae were small, elongated and white to pale in colour. phylogenetically, the 18s rdna nucleotide sequence of m.o ...201527843992
the phylogenetic position of myxoboluscarnaticus (myxozoa, myxosporea, bivalvulida) infecting gill lamellae of cirrhinus mrigala (hamilton, 1822) based on 18s rrna sequence analysis.myxozoans are an economically important group of microscopic parasites best known for the diseases they cause in commercially important fish hosts. the classification of myxosporeans is generally based on the morphology of their myxospores. without molecular data, it is very difficult to identify new or existing species. dna sequence information is therefore, a prerequisite to taxonomic and phylogenic studies of myxosporeans. in the present study, a myxozoan parasite, myxobolus carnaticus, infec ...201527844004
microelectrophoretic study of environmentally induced dna damage in fish and its use for early toxicity screening of freshwater bodies.this study investigates the potential of the comet and micronucleus assays of fish dna as a means of screening the toxicity of aquatic environments. catla catla and cirrhinus mrigala collected from the river chenab in pakistan were used as a case study for the application of comet and micronucleus techniques. comet and micronucleus assays were used to compare dna damage in c. catla and c. mrigala collected from polluted areas of the river chenab and farmed fish. atomic absorption spectrophotomet ...201728213766
lernaea cyprinacea infection in a new host puntius pulchellus in intensive culture system and its control by doramectin.the present study explored the susceptibility of puntius pulchellus subadults to lernaea cyprinacea infection subsequent upon their first introduction to an intensive culture system that had a previous history of lernaea infection and evaluated the efficacy of doramectin against the parasite. all the p. pulchellus introduced to the culture pond got infected demonstrating 100 % susceptibility of this species to lernaea infection. pathological changes caused by the parasite as evidenced by the ext ...201728316399
genetic diversity and population structure of cirrhinus mrigala revealed by mitochondrial atpase 6 gene.the genetic diversity and population structure of cirrhinus mrigala were studied using partial mitochondrial atpase 6 gene. 155 individuals were collected from six distant rivers of peninsular india. a total of 11 haplotypes were found with haplotype diversity value of 0.46711 and nucleotide diversity 0.00154 indicating a low degree of genetic divergence among populations. the result of amova analysis also revealed that 97.53% of the genetic variation contained within populations and 2.47% occur ...201728399684
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