Publications

transcriptomic analysis of clam extrapallial fluids reveals immunity and cytoskeleton alterations in the first week of brown ring disease development.	the brown ring disease is an infection caused by the bacterium vibrio tapetis on the manila clam ruditapes philippinarum. the process of infection, in the extrapallial fluids (epfs) of clams, involves alteration of immune functions, in particular on hemocytes which are the cells responsible of phagocytosis. disorganization of the actin-cytoskeleton in infected clams is a part of what leads to this alteration. this study is the first transcriptomic approach based on collection of extrapallial flu ...	2019	31419531
selection of vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells.	pacific oyster mortality syndrome affects juveniles of crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. vibrio crassostreae (v. crassostreae) has been repeatedly isolated from diseased animals, and the majority of the strains have been demonstrated to be virulent for oysters. in this study, we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by v. crassostreae, while oysters, at an adult stage, were re ...	2019	31390475
dna mini-barcoding of leporids using noninvasive fecal dna samples and its significance for monitoring an invasive species.	introduced in south america at the end of the 19th century, the european hare population has expanded dramatically and now represents a risk to native brazilian forest rabbits. monitoring the invasive lepus europaeus and its coexistence with native sylvilagus brasiliensis is a challenge that can be efficiently addressed by the use of molecular tools. this work describes a set of primers useful for amplifying three mini-barcodes for the molecular identification of both invasive and native leporid ...	2020	32607145
temporal dynamics of infection of cockles cerastoderma edule with the protistan parasite minchinia tapetis (rhizaria: haplosporida) in galicia (nw spain).	uninucleate and binucleate cells and multinucleate plasmodia of a haplosporidan-like protist associated with heavy haemocytic infiltration were observed in histological sections of cockles, cerastoderma edule, from the ría de noia (galicia, nw spain) in the course of a cockle health surveillance programme. molecular assays provided identification of this protist as minchinia tapetis, which we thus record from a new host. prevalence of m. tapetis as high as 93% was recorded but infection intensit ...	2020	32119954
molecular characterization of urosporidium tapetis sp. nov., a haplosporidian hyperparasite infecting metacercariae of parvatrema duboisi (dollfus 1923), a trematode parasite of manila clam ruditapes philippinarum on the west coast of korea.	recently, a putative new hyperparasitic haplosporidian in the genus urosporidium was identified from metacercariae of the trematode parvatrema duboisi infecting manila clam ruditapes philippinarum on the west coast of korea. in this study, we applied small subunit ribosomal dna (ssu rdna) sequences as a marker to substantiate the phylogenetic relationship of the unidentified urosporidium within the order haplosporida. in our phylogenetic analysis, the 1890 bp of ssu rdna sequences obtained were ...	2020	32822733
a proteomic study of resistance to brown ring disease in the manila clam, ruditapes philippinarum.	marine mollusk aquaculture has more than doubled over the past twenty years, accounting for over 15% of total aquaculture production in 2016. infectious disease is one of the main limiting factors to the development of mollusk aquaculture, and the difficulties inherent to combating pathogens through antibiotic therapies or disinfection have led to extensive research on host defense mechanisms and host-pathogen relationships. it has become increasingly clear that characterizing the functional pro ...	2020	32044464
biotic and abiotic factors influencing haplosporidian species distribution in the cockle cerastoderma edule in ireland.	the phylum haplosporidia consists of four genera (minchinia, haplosporidium, urosporidium and bonamia) that are endoparasitic protists of a wide range of marine invertebrates including commercial bivalve species. characterization of haplosporidian species remains a challenge due to their patchy spatial and temporal distributions, host-restricted occurrence, and poorly known life cycles. however, they are commonly associated with significant mortality events in bivalves. due to the recent sporadi ...	2020	32553639
external ph modulation during the growth of vibrio tapetis, the aetiological agent of brown ring disease.	brown ring disease (brd) is an infection of the manila clam ruditapes philippinarum due to the pathogen vibrio tapetis. during brd, clams are facing immunodepression and shell biomineralization alteration. in this paper, we studied the role of ph on the growth of the pathogen and formulated hypothesis on the establishment of brd by v. tapetis.	2020	32395854