| coccidiosis in swine: effect of disinfectants on in vitro sporulation of isospora suis oocysts. | | 1981 | 6914860 |
| coccidiosis in swine: effect of disinfectants on in vitro sporulation of isospora suis oocysts. | | 1981 | 6914860 |
| coccidiosis in swine: a search for extraintestinal stages of isospora suis. | clinical coccidiosis in swine is associated with isospora suis infection of piglets. the endogenous life cycle of i suis in piglets occurs within the superficial epithelium of the small intestine. extraintestinal stages of i suis were not demonstrated in tissues of experimentally infected piglets or mice. feeding these tissues to previously uninfected piglets did not result in oocyst shedding of consequence. extraintestinal stages do not seem to occur in piglets with i suis as in some other isos ... | 1982 | 7064330 |
| coccidiosis in swine: a search for extraintestinal stages of isospora suis. | clinical coccidiosis in swine is associated with isospora suis infection of piglets. the endogenous life cycle of i suis in piglets occurs within the superficial epithelium of the small intestine. extraintestinal stages of i suis were not demonstrated in tissues of experimentally infected piglets or mice. feeding these tissues to previously uninfected piglets did not result in oocyst shedding of consequence. extraintestinal stages do not seem to occur in piglets with i suis as in some other isos ... | 1982 | 7064330 |
| ultrastructural evidence of endodyogeny in isospora suis from pigs. | | 1982 | 7072320 |
| ultrastructural evidence of endodyogeny in isospora suis from pigs. | | 1982 | 7072320 |
| sporogony of isospora suis biester, 1934 of swine. | sporogony of isospora suis of swine is described. unsporulated oocysts were collected immediately after defecation, mixed in 2.5% potassium dichromate solution, incubated at various temperatures, examined at different time intervals, and classified as to stage of development. the uninucleate sporont underwent nuclear division and became binucleated, and then divided to form two round, uninucleate sporoblasts. binucleate sporoblasts elongated to form binucleate sporocysts, each of which then gave ... | 1982 | 7131190 |
| sporogony of isospora suis biester, 1934 of swine. | sporogony of isospora suis of swine is described. unsporulated oocysts were collected immediately after defecation, mixed in 2.5% potassium dichromate solution, incubated at various temperatures, examined at different time intervals, and classified as to stage of development. the uninucleate sporont underwent nuclear division and became binucleated, and then divided to form two round, uninucleate sporoblasts. binucleate sporoblasts elongated to form binucleate sporocysts, each of which then gave ... | 1982 | 7131190 |
| coccidia of feral swine from florida. | fecal samples from 251 free-ranging feral swine (sus scrofa) in florida were examined for coccidia. ten species of coccidia were recovered. in descending order with respect to prevalence, they were eimeria porci, e debliecki, e neodebliecki, e scarbra, e suis, e cerdonis, e polita, e perminuta, isospora suis, and e spinosa. of the swine examined, 87% were passing oocysts and 82% of the infected individuals were shedding at least 2 species of coccidia. all age classes included infected individual ... | 1982 | 7174441 |
| coccidia of feral swine from florida. | fecal samples from 251 free-ranging feral swine (sus scrofa) in florida were examined for coccidia. ten species of coccidia were recovered. in descending order with respect to prevalence, they were eimeria porci, e debliecki, e neodebliecki, e scarbra, e suis, e cerdonis, e polita, e perminuta, isospora suis, and e spinosa. of the swine examined, 87% were passing oocysts and 82% of the infected individuals were shedding at least 2 species of coccidia. all age classes included infected individual ... | 1982 | 7174441 |
| demonstration of immunity against isospora suis in swine. | piglets naturally exposed or experimentally infected with isospora suis oocysts were given challenge doses of oocysts to determine the extent of development of immune resistance. piglets in both studies shed low numbers of, or no detectable oocysts, following challenge. administration of methylprednisolone acetate failed to induce oocyst shedding in previously infected piglets. piglets rechallenged with i. suis following steroid injections also failed to shed significant numbers of oocysts sugge ... | 1982 | 7201193 |
| demonstration of immunity against isospora suis in swine. | piglets naturally exposed or experimentally infected with isospora suis oocysts were given challenge doses of oocysts to determine the extent of development of immune resistance. piglets in both studies shed low numbers of, or no detectable oocysts, following challenge. administration of methylprednisolone acetate failed to induce oocyst shedding in previously infected piglets. piglets rechallenged with i. suis following steroid injections also failed to shed significant numbers of oocysts sugge ... | 1982 | 7201193 |
| [effect of a monoinfection with isospora suis and its treatment using halofuginone and lasalocid on breeding achievement, digestion coefficients and composition of whole body cadavers of early weaned piglets]. | | 1981 | 7213333 |
| [effect of a monoinfection with isospora suis and its treatment using halofuginone and lasalocid on breeding achievement, digestion coefficients and composition of whole body cadavers of early weaned piglets]. | | 1981 | 7213333 |
| [the development of weaned piglets experimentally infected with isospora suis as a model for the effect of lasalocid and halofuginone on coccidia (author's transl)]. | piglets which were early-weaned at the age of 21.7 days and experimentally monoinfected with oocysts of isospora suis showed distinct reductions in zootechnical criteria during an experimental period of 4 weeks. the daily liveweight gains in the infected piglets (group b) was 19.7% lower than in the control group a, which was free of coccidia. comparative photographs with the rem showed serious lesions in the small intestine of infected piglets, which are thought to be mainly responsible for the ... | 1981 | 7223136 |
| [the development of weaned piglets experimentally infected with isospora suis as a model for the effect of lasalocid and halofuginone on coccidia (author's transl)]. | piglets which were early-weaned at the age of 21.7 days and experimentally monoinfected with oocysts of isospora suis showed distinct reductions in zootechnical criteria during an experimental period of 4 weeks. the daily liveweight gains in the infected piglets (group b) was 19.7% lower than in the control group a, which was free of coccidia. comparative photographs with the rem showed serious lesions in the small intestine of infected piglets, which are thought to be mainly responsible for the ... | 1981 | 7223136 |
| porcine neonatal coccidiosis. | coccidia were identified in intestinal sections from 82 piglets comprising 37 consignments from 34 farms, and represented a yearly increasing incidence in the three years 1978 to 1980. piglets were primarily from medium to large farms with intensive, continuous-farrowing, confinement-rearing programs. piglets, usually five days to 15 days old, had yellow, fluid diarrhea, became unthrifty and sometimes died. in six piglets from two farms, a green, adherent, fibrinonecrotic membrane was seen throu ... | 1981 | 7343074 |
| porcine neonatal coccidiosis. | coccidia were identified in intestinal sections from 82 piglets comprising 37 consignments from 34 farms, and represented a yearly increasing incidence in the three years 1978 to 1980. piglets were primarily from medium to large farms with intensive, continuous-farrowing, confinement-rearing programs. piglets, usually five days to 15 days old, had yellow, fluid diarrhea, became unthrifty and sometimes died. in six piglets from two farms, a green, adherent, fibrinonecrotic membrane was seen throu ... | 1981 | 7343074 |
| isospora suis enteritis in piglets. | a species of porcine coccidia, isospora suis, when inoculated into piglets, produced diarrhea, dehydration, weight loss and death. gross lesions were characterized by a fibrino-necrotic membrane within the jejunum and ileum. villous atrophy and variable erosion, often with an adhered necrotic membrane, were seen microscopically. asexual and sexual stages of i. suis were seen within the intestinal epithelium and oocysts were recovered from the feces. the severity of clinical disease and lesions w ... | 1980 | 7352366 |
| isospora suis enteritis in piglets. | a species of porcine coccidia, isospora suis, when inoculated into piglets, produced diarrhea, dehydration, weight loss and death. gross lesions were characterized by a fibrino-necrotic membrane within the jejunum and ileum. villous atrophy and variable erosion, often with an adhered necrotic membrane, were seen microscopically. asexual and sexual stages of i. suis were seen within the intestinal epithelium and oocysts were recovered from the feces. the severity of clinical disease and lesions w ... | 1980 | 7352366 |
| endogenous development of the swine coccidium, isospora suis biester 1934. | the endogenous development of isospora suis biester 1934 is described in piglets inoculated with 150,000 or 200,000 sporulated oocysts. endogenous stages developed within villous epithelial cells throughout the small intestine. two distinct types of meronts were seen in tissue sections. type i meronts, which were seen at 3 days postinoculation, were binucleate, elongate, and 10.5 by 4.7 micron. they produced two to 14 type i merozoites per parasitophorous vacuole. type i merozoites were 10.0 by ... | 1980 | 7463245 |
| endogenous development of the swine coccidium, isospora suis biester 1934. | the endogenous development of isospora suis biester 1934 is described in piglets inoculated with 150,000 or 200,000 sporulated oocysts. endogenous stages developed within villous epithelial cells throughout the small intestine. two distinct types of meronts were seen in tissue sections. type i meronts, which were seen at 3 days postinoculation, were binucleate, elongate, and 10.5 by 4.7 micron. they produced two to 14 type i merozoites per parasitophorous vacuole. type i merozoites were 10.0 by ... | 1980 | 7463245 |
| the use of toltrazuril for the prevention of coccidiosis in piglets before weaning. | to determine the efficacy of toltrazuril as a prophylactic treatment for coccidiosis in piglets caused by isospora suis (i suis), a single 1.0 ml dose of toltrazuril was administered orally to 1056 piglets between 3 and 6 days of age, in 5 piggeries. prophylactic treatment of piglets reduced the occurrence of coccidiosis in litters from 71% to 22%. the number of antibacterial treatments given and the number of piglets affected per litter were also significantly reduced, resulting in some improve ... | 1995 | 7646378 |
| the use of toltrazuril for the prevention of coccidiosis in piglets before weaning. | to determine the efficacy of toltrazuril as a prophylactic treatment for coccidiosis in piglets caused by isospora suis (i suis), a single 1.0 ml dose of toltrazuril was administered orally to 1056 piglets between 3 and 6 days of age, in 5 piggeries. prophylactic treatment of piglets reduced the occurrence of coccidiosis in litters from 71% to 22%. the number of antibacterial treatments given and the number of piglets affected per litter were also significantly reduced, resulting in some improve ... | 1995 | 7646378 |
| the prevalence of isospora suis and strongyloides ransomi in suckling piglets in the netherlands. | faecal samples from suckling piglets from 113 litters on 25 farms in the netherlands were examined to study the prevalence of gastrointestinal parasites, in particular strongyloides ransomi and isospora suis. s. ransomi was not found but i. suis was demonstrated in 17 of the 25 farms and in 41 out of 77 litters of these farms (53%). no other parasites were found. on the basis of these results, a longitudinal study on the incidence and significance of i. suis was performed on 10 farms (5 litters/ ... | 1994 | 7740744 |
| the prevalence of isospora suis and strongyloides ransomi in suckling piglets in the netherlands. | faecal samples from suckling piglets from 113 litters on 25 farms in the netherlands were examined to study the prevalence of gastrointestinal parasites, in particular strongyloides ransomi and isospora suis. s. ransomi was not found but i. suis was demonstrated in 17 of the 25 farms and in 41 out of 77 litters of these farms (53%). no other parasites were found. on the basis of these results, a longitudinal study on the incidence and significance of i. suis was performed on 10 farms (5 litters/ ... | 1994 | 7740744 |
| shedding of oocysts in piglets experimentally infected with isospora suis. | forty-seven piglets were inoculated with doses of 100 to 50,000 sporulated oocysts of isospora suis. after 5-7 days oocysts were found in faeces. the patent period extended from 8 to 16 days. the shedding of oocysts showed a cyclic pattern with 2-3 peaks separated by intervals of approximately 5 days. subpatent periods were often seen between the peaks. the level of oocyst shedding during the initial days of the patent period reflected, to some extent, the inoculation dose. however, a maximum of ... | 1994 | 7942382 |
| shedding of oocysts in piglets experimentally infected with isospora suis. | forty-seven piglets were inoculated with doses of 100 to 50,000 sporulated oocysts of isospora suis. after 5-7 days oocysts were found in faeces. the patent period extended from 8 to 16 days. the shedding of oocysts showed a cyclic pattern with 2-3 peaks separated by intervals of approximately 5 days. subpatent periods were often seen between the peaks. the level of oocyst shedding during the initial days of the patent period reflected, to some extent, the inoculation dose. however, a maximum of ... | 1994 | 7942382 |
| detection of neospora caninum in tissue sections using a murine monoclonal antibody. | a murine monoclonal antibody (mab 6g7), isotype igg2a, produced against tachyzoites of neospora caninum (isolate nc-1) reacted specifically with tachyzoites of n. caninum in an indirect immunofluorescent antibody test. mab 6g7 did not react with tachyzoites of toxoplasma gondii, sporozoites of isospora suis, eimeria bovis, or e. tenella, or merozoites of e. bovis in the indirect immunofluorescent antibody test. mab 6g7 reacted positively with both tachyzoites and bradyzoites of n. caninum in an ... | 1993 | 8286458 |
| detection of neospora caninum in tissue sections using a murine monoclonal antibody. | a murine monoclonal antibody (mab 6g7), isotype igg2a, produced against tachyzoites of neospora caninum (isolate nc-1) reacted specifically with tachyzoites of n. caninum in an indirect immunofluorescent antibody test. mab 6g7 did not react with tachyzoites of toxoplasma gondii, sporozoites of isospora suis, eimeria bovis, or e. tenella, or merozoites of e. bovis in the indirect immunofluorescent antibody test. mab 6g7 reacted positively with both tachyzoites and bradyzoites of n. caninum in an ... | 1993 | 8286458 |
| ribosomal rna sequences for the specific detection of toxoplasma gondii by hybridization assay. | we describe the development of five oligonucleotide probes which target the small sub-unit rrna of toxoplasma gondii. these probes bind to different regions within the rrna and all were effective in producing a detectable signal with as little as 10 ng of total cellular rna. one probe in particular, tgb, was able to detect as little as 1 ng of rna and was the most specific. a sixth probe, previously developed for hybridization with sarcocystis spp., was evaluated for its specificity and was show ... | 1993 | 8321257 |
| ribosomal rna sequences for the specific detection of toxoplasma gondii by hybridization assay. | we describe the development of five oligonucleotide probes which target the small sub-unit rrna of toxoplasma gondii. these probes bind to different regions within the rrna and all were effective in producing a detectable signal with as little as 10 ng of total cellular rna. one probe in particular, tgb, was able to detect as little as 1 ng of rna and was the most specific. a sixth probe, previously developed for hybridization with sarcocystis spp., was evaluated for its specificity and was show ... | 1993 | 8321257 |
| ultrastructure of isospora suis during excystation and attempts to demonstrate extraintestinal stages in mice. | transmission electron microscopy was used to examine the structure of the oocysts, sporocysts and sporozoites of isospora suis during in vitro excystation. oocysts were ground in a teflon-coated tissue grinder to free most sporocysts and to allow for exposure of oocysts and sporocysts to excystation medium. the suspension of oocysts and sporocysts was incubated at 37 degrees c for 0-45 min in excystation medium. after incubation, the intact oocysts and sporocysts, excysted sporocysts, and sporoz ... | 1993 | 8333128 |
| ultrastructure of isospora suis during excystation and attempts to demonstrate extraintestinal stages in mice. | transmission electron microscopy was used to examine the structure of the oocysts, sporocysts and sporozoites of isospora suis during in vitro excystation. oocysts were ground in a teflon-coated tissue grinder to free most sporocysts and to allow for exposure of oocysts and sporocysts to excystation medium. the suspension of oocysts and sporocysts was incubated at 37 degrees c for 0-45 min in excystation medium. after incubation, the intact oocysts and sporocysts, excysted sporocysts, and sporoz ... | 1993 | 8333128 |
| studies on preweaning piglet diarrhoea. | the aim of this study was to determine the cause and risk factors involved in preweaning piglet diarrhoea. faecal samples from 2380 diarrhoeic piglets, 5 to 30 days of age, were examined for enteropathogens. isospora suis oocysts were detected in 53.8% of samples, escherichia coli in 18.2% and rotavirus in 16.9%. i suis had the widest distribution, being present on 70.9% of 151 piggeries. the onset of diarrhoea occurred mainly between 7 and 14 days (77.5%), peaking at 10 days. records of 4086 li ... | 1993 | 8368968 |
| studies on preweaning piglet diarrhoea. | the aim of this study was to determine the cause and risk factors involved in preweaning piglet diarrhoea. faecal samples from 2380 diarrhoeic piglets, 5 to 30 days of age, were examined for enteropathogens. isospora suis oocysts were detected in 53.8% of samples, escherichia coli in 18.2% and rotavirus in 16.9%. i suis had the widest distribution, being present on 70.9% of 151 piggeries. the onset of diarrhoea occurred mainly between 7 and 14 days (77.5%), peaking at 10 days. records of 4086 li ... | 1993 | 8368968 |
| [the epizootiology and pathogenic significance of infections with isospora suis in ten piglet production operations in nordrhein-westfalen]. | from july 1993 to june 1994 2036 fecal samples from 196 litters from 10 pig farms were collected. three or four piglets from each litter and the respective sows were examined 3 times at 2, 3 and 4 weeks after farrowing. i. suis was detected in all 10 farms. the rate of infection of the litters was 62.2%. 71.6% of all litters with diarrheic problems were i. suis positive. the percentage of i. suis in the feces of piglets was 17.4% at the age of 2 weeks, 41.3% at the age of 3 weeks and 39.1% at th ... | 1996 | 8765537 |
| [the epizootiology and pathogenic significance of infections with isospora suis in ten piglet production operations in nordrhein-westfalen]. | from july 1993 to june 1994 2036 fecal samples from 196 litters from 10 pig farms were collected. three or four piglets from each litter and the respective sows were examined 3 times at 2, 3 and 4 weeks after farrowing. i. suis was detected in all 10 farms. the rate of infection of the litters was 62.2%. 71.6% of all litters with diarrheic problems were i. suis positive. the percentage of i. suis in the feces of piglets was 17.4% at the age of 2 weeks, 41.3% at the age of 3 weeks and 39.1% at th ... | 1996 | 8765537 |
| prevalence of porcine neonatal isosporosis in brazil. | the prevalence of isospora suis and clinical signs of isosporosis were observed in 33 swine farms from 20 sites in the southeastern state of são paulo, brazil. the study was performed by collecting 177 faecal samples from nursing and weaned piglets. a history of clinical neonatal isosporosis, as well as the type of farrowing and nursery houses and the pig management in the farms were correlated to the prevalence of i. suis oocysts. six faecal samples were collected in each of the farms (two from ... | 1996 | 9017865 |
| prevalence of porcine neonatal isosporosis in brazil. | the prevalence of isospora suis and clinical signs of isosporosis were observed in 33 swine farms from 20 sites in the southeastern state of são paulo, brazil. the study was performed by collecting 177 faecal samples from nursing and weaned piglets. a history of clinical neonatal isosporosis, as well as the type of farrowing and nursery houses and the pig management in the farms were correlated to the prevalence of i. suis oocysts. six faecal samples were collected in each of the farms (two from ... | 1996 | 9017865 |
| immunohistochemical diagnosis of toxoplasma gondii: potential for cross-reactivity with neospora caninum. | a monoclonal antibody (mab 3f5) was compared with a commercial polyclonal antibody for specificity for toxoplasma gondii and cross-reactivity with neospora caninum in paraffin-embedded tissue sections. both antibody preparations reliably recognized t. gondii tachyzoites (rh isolate) in animal tissues but did not always label tissue cysts (me-49 isolate). the commercial antibody strongly cross-reacted with n. caninum tachyzoites, but mab 3f5 did not when the immunoperoxidase, immunofluorescence, ... | 1997 | 9194824 |
| immunohistochemical diagnosis of toxoplasma gondii: potential for cross-reactivity with neospora caninum. | a monoclonal antibody (mab 3f5) was compared with a commercial polyclonal antibody for specificity for toxoplasma gondii and cross-reactivity with neospora caninum in paraffin-embedded tissue sections. both antibody preparations reliably recognized t. gondii tachyzoites (rh isolate) in animal tissues but did not always label tissue cysts (me-49 isolate). the commercial antibody strongly cross-reacted with n. caninum tachyzoites, but mab 3f5 did not when the immunoperoxidase, immunofluorescence, ... | 1997 | 9194824 |
| the time course of the specific antibody response by various elisas in pigs experimentally infected with toxoplasma gondii. | with the aim of developing routine serological tests for monitoring the toxoplasma infection status of danish swine herds, four elisas based on tachyzoite antigen were set up: (1) an indirect elisa for igg-antibody; (2) a blocking elisa for antibody to the membrane antigen, p30; (3) an indirect elisa for igm; (4) a reverse, antibody-catching igm-elisa. groups of pigs (number between 6 and 10) were inoculated with tachyzoites of the rh-strain, tissue cysts of two complete strains, or oocysts in t ... | 1997 | 9231984 |
| the time course of the specific antibody response by various elisas in pigs experimentally infected with toxoplasma gondii. | with the aim of developing routine serological tests for monitoring the toxoplasma infection status of danish swine herds, four elisas based on tachyzoite antigen were set up: (1) an indirect elisa for igg-antibody; (2) a blocking elisa for antibody to the membrane antigen, p30; (3) an indirect elisa for igm; (4) a reverse, antibody-catching igm-elisa. groups of pigs (number between 6 and 10) were inoculated with tachyzoites of the rh-strain, tissue cysts of two complete strains, or oocysts in t ... | 1997 | 9231984 |
| examination of extraintestinal tissue cysts of isospora belli. | relapse is common in immunocompetent and immunosuppressed humans infected with isospora belli and is believed to be associated with the presence of extraintestinal stages. in the present study, we examined this important stage in an aids patient using histological, immunohistological, histochemical, and ultrastructural methods to better understand the development and structure of this stage and to develop better means of detecting infections. antisera made in rabbits to isospora suis, toxoplasma ... | 1997 | 9267401 |
| examination of extraintestinal tissue cysts of isospora belli. | relapse is common in immunocompetent and immunosuppressed humans infected with isospora belli and is believed to be associated with the presence of extraintestinal stages. in the present study, we examined this important stage in an aids patient using histological, immunohistological, histochemical, and ultrastructural methods to better understand the development and structure of this stage and to develop better means of detecting infections. antisera made in rabbits to isospora suis, toxoplasma ... | 1997 | 9267401 |
| phylogenetic analysis of coccidia based on 18s rdna sequence comparison indicates that isospora is most closely related to toxoplasma and neospora. | the phylogenetic relationships and taxonomic affinities of coccidia with isosporan-type oocysts have been unclear as overlapping characters, recently discovered life cycle features, and even recently discovered taxa, continue to be incorporated into biological classifications of the group. we determined the full or partial 18s ribosomal rna gene sequences of three mammalian isospora spp., isospora felis, isospora ohioensis and isospora suis, and a sarcocystis sp. of a rattlesnake, and used these ... | 1998 | 9561772 |
| phylogenetic analysis of coccidia based on 18s rdna sequence comparison indicates that isospora is most closely related to toxoplasma and neospora. | the phylogenetic relationships and taxonomic affinities of coccidia with isosporan-type oocysts have been unclear as overlapping characters, recently discovered life cycle features, and even recently discovered taxa, continue to be incorporated into biological classifications of the group. we determined the full or partial 18s ribosomal rna gene sequences of three mammalian isospora spp., isospora felis, isospora ohioensis and isospora suis, and a sarcocystis sp. of a rattlesnake, and used these ... | 1998 | 9561772 |
| complete development of the porcine coccidium isospora suis biester, 1934 in cell cultures. | development from inoculated sporozoites to unsporulated oocysts of isospora suis biester, 1934 is described in a swine testicular (st) cell line. sporozoites penetrated st cells within 1 hr postinoculation (pi). development was initially by endodyogeny to produce binucleate type i meronts and type i merozoites. division by endodyogeny continued during the 13-day observation period and type i merozoites were the developmental stages most abundant at observation periods >3 days pi. mutinucleate ty ... | 1998 | 9645875 |
| complete development of the porcine coccidium isospora suis biester, 1934 in cell cultures. | development from inoculated sporozoites to unsporulated oocysts of isospora suis biester, 1934 is described in a swine testicular (st) cell line. sporozoites penetrated st cells within 1 hr postinoculation (pi). development was initially by endodyogeny to produce binucleate type i meronts and type i merozoites. division by endodyogeny continued during the 13-day observation period and type i merozoites were the developmental stages most abundant at observation periods >3 days pi. mutinucleate ty ... | 1998 | 9645875 |
| intestinal parasites in swine in the nordic countries: prevalence and geographical distribution. | in denmark (dk), finland (fin), iceland (i), norway (n), and sweden (s), 516 swine herds were randomly selected in 1986-1988. individual faecal analyses (mean: 27.9 per herd) from eight age categories of swine showed that ascaris suum, oesophagostomum spp., isospora suis, and eimeria spp. were common, while trichuris suis and strongyloides ransomi-like eggs occurred sporadically. large fatteners and gilts were most frequently infected with a. suum with maximum prevalences of 25-35% in dk, n and ... | 1998 | 9650867 |
| intestinal parasites in swine in the nordic countries: prevalence and geographical distribution. | in denmark (dk), finland (fin), iceland (i), norway (n), and sweden (s), 516 swine herds were randomly selected in 1986-1988. individual faecal analyses (mean: 27.9 per herd) from eight age categories of swine showed that ascaris suum, oesophagostomum spp., isospora suis, and eimeria spp. were common, while trichuris suis and strongyloides ransomi-like eggs occurred sporadically. large fatteners and gilts were most frequently infected with a. suum with maximum prevalences of 25-35% in dk, n and ... | 1998 | 9650867 |
| diarrhoea in nursing piglets associated with coccidiosis: prevalence, microscopic lesions and coexisting microorganisms. | a retrospective study was made of natural infections with isospora suis in nursing piglets, recorded from april 1994 to may 1997, to determine the prevalence, microscopical lesions and other microorganisms associated with coccidiosis. one hundred and five (17.3 per cent) of the 605 nursing piglets submitted from 304 pig farms were diagnosed positive for coccidiosis. the affected piglets were from seven to 20 days old, with a mean age of 11.1 days. coccidiosis occurred in each year but the incide ... | 1998 | 9807791 |
| diarrhoea in nursing piglets associated with coccidiosis: prevalence, microscopic lesions and coexisting microorganisms. | a retrospective study was made of natural infections with isospora suis in nursing piglets, recorded from april 1994 to may 1997, to determine the prevalence, microscopical lesions and other microorganisms associated with coccidiosis. one hundred and five (17.3 per cent) of the 605 nursing piglets submitted from 304 pig farms were diagnosed positive for coccidiosis. the affected piglets were from seven to 20 days old, with a mean age of 11.1 days. coccidiosis occurred in each year but the incide ... | 1998 | 9807791 |
| experimental infection of swine by isospora suis biester 1934 for species confirmation. | a survey of isospora suis performed in 177 faecal samples from 30 swine farms detected thin wall type i. suis oocysts in seven samples. this type of oocyst measuring 23.9 by 20.7 microns had a retracted thin wall similar to that of the genus sarcocystis. this type of oocysts, isolated from four different faecal samples, was inoculated in four-five-days-old piglets free of contamination in order to verify the life cycle and pathogenicity of the species. the pigs were kept in individual metal cage ... | 1998 | 9921315 |
| experimental infection of swine by isospora suis biester 1934 for species confirmation. | a survey of isospora suis performed in 177 faecal samples from 30 swine farms detected thin wall type i. suis oocysts in seven samples. this type of oocyst measuring 23.9 by 20.7 microns had a retracted thin wall similar to that of the genus sarcocystis. this type of oocysts, isolated from four different faecal samples, was inoculated in four-five-days-old piglets free of contamination in order to verify the life cycle and pathogenicity of the species. the pigs were kept in individual metal cage ... | 1998 | 9921315 |
| role of acquired immunity and natural age resistance on course of isospora suis coccidiosis in nursing piglets. | thirty-two piglets from three litters were experimentally inoculated with 200000 sporulated oocysts of isospora suis at 3 days of age and/or rechallenged at 19 days of age or primary inoculated at 19 days of age, to compare the role of acquired immunity and natural age resistance on the course of coccidiosis. twelve piglets were not inoculated and served as a control. following challenge, the signs of coccidiosis characterised by clinical symptoms, oocysts shedding and weekly weights were simila ... | 1999 | 10321581 |
| role of acquired immunity and natural age resistance on course of isospora suis coccidiosis in nursing piglets. | thirty-two piglets from three litters were experimentally inoculated with 200000 sporulated oocysts of isospora suis at 3 days of age and/or rechallenged at 19 days of age or primary inoculated at 19 days of age, to compare the role of acquired immunity and natural age resistance on the course of coccidiosis. twelve piglets were not inoculated and served as a control. following challenge, the signs of coccidiosis characterised by clinical symptoms, oocysts shedding and weekly weights were simila ... | 1999 | 10321581 |
| occurrence of isospora suis in larger piglet production units and on specialized piglet rearing farms. | mixed fecal samples of 264 litters from five piglet production farms (155-238 sows/farm) were investigated three times during the suckling period for the occurrence of isospora suis over the period of 1 year. on all five farms isopora suis was found to be a common endoparasite with infection rates being highest in litters of 3-4 weeks of age. by the end of the third investigation period the cumulative infection rate was 53.8% of the litters ranging from 20.0% to 81.5% for the single farms. durin ... | 1999 | 10384903 |
| occurrence of isospora suis in larger piglet production units and on specialized piglet rearing farms. | mixed fecal samples of 264 litters from five piglet production farms (155-238 sows/farm) were investigated three times during the suckling period for the occurrence of isospora suis over the period of 1 year. on all five farms isopora suis was found to be a common endoparasite with infection rates being highest in litters of 3-4 weeks of age. by the end of the third investigation period the cumulative infection rate was 53.8% of the litters ranging from 20.0% to 81.5% for the single farms. durin ... | 1999 | 10384903 |
| parasites in cross-bred pigs in the upper east region of ghana. | a cross-sectional study was carried out in the upper east region (uer) of ghana in order to estimate the prevalence of parasitic infections in local cross-bred pigs. out of 60 villages with a human population of 200-1000 inhabitants, 10 villages were randomly selected for the study. the number of pigs varied from 50 to 200 pigs per village. in total 259 faecal samples from growers were collected and examined. ninety-one percent of the animals excreted parasite eggs. among these the prevalence of ... | 1999 | 10628701 |
| parasites in cross-bred pigs in the upper east region of ghana. | a cross-sectional study was carried out in the upper east region (uer) of ghana in order to estimate the prevalence of parasitic infections in local cross-bred pigs. out of 60 villages with a human population of 200-1000 inhabitants, 10 villages were randomly selected for the study. the number of pigs varied from 50 to 200 pigs per village. in total 259 faecal samples from growers were collected and examined. ninety-one percent of the animals excreted parasite eggs. among these the prevalence of ... | 1999 | 10628701 |
| [endoparasites in swine in different age groups and management systems]. | in different age groups endoparasites are of varying importance. sows are predominantly infected with gastrointestinal strongyles (mainly oesophagostomum spp.), ascaris suum and eimeriae, to a lesset extent with trichuris suis, hyostrongylus rubidus, strongyloides ransomi or isospora suis, while in suckling piglets i. suis and occasionally s. ransomi frequently occur. weaners and fatteners are infected with coccidia and gastrointestinal strongyles, later with a. suum. whipworms are also found oc ... | 2000 | 10816911 |
| [endoparasites in swine in different age groups and management systems]. | in different age groups endoparasites are of varying importance. sows are predominantly infected with gastrointestinal strongyles (mainly oesophagostomum spp.), ascaris suum and eimeriae, to a lesset extent with trichuris suis, hyostrongylus rubidus, strongyloides ransomi or isospora suis, while in suckling piglets i. suis and occasionally s. ransomi frequently occur. weaners and fatteners are infected with coccidia and gastrointestinal strongyles, later with a. suum. whipworms are also found oc ... | 2000 | 10816911 |
| immunity against isospora suis in nursing piglets. | | 2000 | 11068822 |
| immunity against isospora suis in nursing piglets. | | 2000 | 11068822 |
| pcr-based differentiation of three porcine eimeria species and isospora suis. | isospora suis and eimeria are frequent coccidian parasites of pigs. the unsporulated oocysts of eimeria species and of i. suis are difficult to differentiate. therefore, a species-specific pcr was developed. pcr products were amplified from eimeria polita, eimeria porci, and eimeria scabra using primers from the conserved 18s rrna regions and were subsequently sequenced. based on variable sequence regions, primers were constructed for the differentiation of the three eimeria species and i. suis. ... | 2001 | 11163694 |
| pcr-based differentiation of three porcine eimeria species and isospora suis. | isospora suis and eimeria are frequent coccidian parasites of pigs. the unsporulated oocysts of eimeria species and of i. suis are difficult to differentiate. therefore, a species-specific pcr was developed. pcr products were amplified from eimeria polita, eimeria porci, and eimeria scabra using primers from the conserved 18s rrna regions and were subsequently sequenced. based on variable sequence regions, primers were constructed for the differentiation of the three eimeria species and i. suis. ... | 2001 | 11163694 |
| prevalence of enteropathogens in suckling and weaned piglets with diarrhoea in southern germany. | faecal samples from suckling (n = 205) and weaned piglets (n = 82) with diarrhoea from 24 farms in southern germany were examined for shedding of important metazoic parasitic, viral and bacterial pathogens using culture, microscopic and electronmicroscopic methods. escherichia coli isolates were tested further for the enterotoxin genes est-ia and elt-i by colony blot hybridization. isospora suis was diagnosed in 26.9% and cryptosporidium parvum in 1.4% of the piglets investigated. the proportion ... | 2001 | 11315526 |
| prevalence of enteropathogens in suckling and weaned piglets with diarrhoea in southern germany. | faecal samples from suckling (n = 205) and weaned piglets (n = 82) with diarrhoea from 24 farms in southern germany were examined for shedding of important metazoic parasitic, viral and bacterial pathogens using culture, microscopic and electronmicroscopic methods. escherichia coli isolates were tested further for the enterotoxin genes est-ia and elt-i by colony blot hybridization. isospora suis was diagnosed in 26.9% and cryptosporidium parvum in 1.4% of the piglets investigated. the proportion ... | 2001 | 11315526 |
| autofluorescence microscopy for the detection of nematode eggs and protozoa, in particular isospora suis, in swine faeces. | parasites from swine faeces were examined for autofluorescence. oocysts of eimeria polita, e. scabra and isospora suis, cysts of balantidium coli and eggs of oesophagostomum dentatum, strongyloides ransomi and trichuris suis (but not those of ascaris suum) emitted light after excitation with uv light. i. suis oocyst counts in mcmaster chambers utilising autofluorescence were compared to those from conventional bright field microscopy. similarly, faecal smears containing i. suis were examined usi ... | 2001 | 11403385 |
| autofluorescence microscopy for the detection of nematode eggs and protozoa, in particular isospora suis, in swine faeces. | parasites from swine faeces were examined for autofluorescence. oocysts of eimeria polita, e. scabra and isospora suis, cysts of balantidium coli and eggs of oesophagostomum dentatum, strongyloides ransomi and trichuris suis (but not those of ascaris suum) emitted light after excitation with uv light. i. suis oocyst counts in mcmaster chambers utilising autofluorescence were compared to those from conventional bright field microscopy. similarly, faecal smears containing i. suis were examined usi ... | 2001 | 11403385 |
| the role of isospora suis as a pathogen in conventional piglet production in germany. | in order to evaluate the prevalence of isospora suis in conventional piglet production in germany, pooled faecal samples from 327 pig litters from 18 pig production units (20-320 sows each) were examined. at least 10 litters from each farm were investigated. i. suis was present on 83% of the farms and 42.5% of the litters, the infection rate being highest in the third week of age (48.2%). i. suis was found more frequently in samples of diarrhoea than in firm faeces (49.2% compared to 22.2%). twe ... | 2002 | 12069269 |
| the role of isospora suis as a pathogen in conventional piglet production in germany. | in order to evaluate the prevalence of isospora suis in conventional piglet production in germany, pooled faecal samples from 327 pig litters from 18 pig production units (20-320 sows each) were examined. at least 10 litters from each farm were investigated. i. suis was present on 83% of the farms and 42.5% of the litters, the infection rate being highest in the third week of age (48.2%). i. suis was found more frequently in samples of diarrhoea than in firm faeces (49.2% compared to 22.2%). twe ... | 2002 | 12069269 |
| lectin histochemistry for glycoconjugates in the small intestines of piglets naturally infected with isospora suis. | composition of glycoconjugates was examined in small intestines naturally infected with isospora suis in preweaned pigs by use of 21 biotinylated-labeled lectins with avidin-biotin-peroxidase complex method. as compared with control pig, staining of 18 lectins altered in jejunal villus brush border and goblet cells of pigs naturally infected with i. suis. these results indicate that i. suis infection alters carbohydrate residues on the jejunal intestines. | 2003 | 12679572 |
| lectin histochemistry for glycoconjugates in the small intestines of piglets naturally infected with isospora suis. | composition of glycoconjugates was examined in small intestines naturally infected with isospora suis in preweaned pigs by use of 21 biotinylated-labeled lectins with avidin-biotin-peroxidase complex method. as compared with control pig, staining of 18 lectins altered in jejunal villus brush border and goblet cells of pigs naturally infected with i. suis. these results indicate that i. suis infection alters carbohydrate residues on the jejunal intestines. | 2003 | 12679572 |
| parasitological and morphological findings in porcine isosporosis after treatment with symmetrical triazintriones. | neonatal porcine isosporosis is known to cause serious economic losses in piglet farms by causing severe enteritis with dehydration, weight loss and reduced development in the affected animals, predominantly during the first weeks of life. in the present study, piglets experimentally infected with isospora suis were treated with bay vi 9143, a symmetrical triazintrione, at different days post-infection. as shown by clinical and pathological examinations, bay vi 9143 is effective against the asex ... | 2003 | 12856173 |
| parasitological and morphological findings in porcine isosporosis after treatment with symmetrical triazintriones. | neonatal porcine isosporosis is known to cause serious economic losses in piglet farms by causing severe enteritis with dehydration, weight loss and reduced development in the affected animals, predominantly during the first weeks of life. in the present study, piglets experimentally infected with isospora suis were treated with bay vi 9143, a symmetrical triazintrione, at different days post-infection. as shown by clinical and pathological examinations, bay vi 9143 is effective against the asex ... | 2003 | 12856173 |
| population biology studies on isospora suis in piglets. | | 2003 | 12928890 |
| population biology studies on isospora suis in piglets. | | 2003 | 12928890 |
| studies on the efficacy of toltrazuril, diclazuril and sulphadimidine against artificial infections with isospora suis in piglets. | | 2003 | 12928891 |
| studies on the efficacy of toltrazuril, diclazuril and sulphadimidine against artificial infections with isospora suis in piglets. | | 2003 | 12928891 |
| the role of isospora suis in the ethiology of diarrhoea in suckling piglets. | | 2003 | 12928892 |
| the role of isospora suis in the ethiology of diarrhoea in suckling piglets. | | 2003 | 12928892 |
| detection of isospora suis (biester and murray 1934) in piglet faeces--comparison of microscopy and pcr. | | 2004 | 15107041 |
| detection of isospora suis (biester and murray 1934) in piglet faeces--comparison of microscopy and pcr. | | 2004 | 15107041 |
| survey of intestinal parasites in pigs from intensive farms in guangdong province, people's republic of china. | the prevalence of intestinal parasites was investigated in intensive pig farms in guangdong province, china between july 2000 and july 2002. faecal samples from 3636 pigs (both sexes and five age groups) from 38 representative intensive pig farms employing different parasite control strategies were examined for the presence of helminth ova and protozoan oocysts, cysts and/or trophozoites using standard techniques. of the 3636 pigs sampled, 209 (5.7%) were infected with trichuris suis, 189 (5.2%) ... | 2004 | 15710534 |
| survey of intestinal parasites in pigs from intensive farms in guangdong province, people's republic of china. | the prevalence of intestinal parasites was investigated in intensive pig farms in guangdong province, china between july 2000 and july 2002. faecal samples from 3636 pigs (both sexes and five age groups) from 38 representative intensive pig farms employing different parasite control strategies were examined for the presence of helminth ova and protozoan oocysts, cysts and/or trophozoites using standard techniques. of the 3636 pigs sampled, 209 (5.7%) were infected with trichuris suis, 189 (5.2%) ... | 2004 | 15710534 |
| occurrence of isospora suis in germany, switzerland and austria. | nationwide surveys for the occurrence of isospora suis were carried out in germany, austria and switzerland including a questionnaire regarding herd size, health status and management practices and a coccidiosis sampling kit for pooled faecal samples from litters of suckling piglets. a total of 184 veterinary practices participated in the survey and returned 1745 samples (331 kits) from 324 farms in the north (n = 98), south (n = 84), centre/east (n = 42) and west (n = 10) of germany, austria (n ... | 2005 | 15752269 |
| occurrence of isospora suis in germany, switzerland and austria. | nationwide surveys for the occurrence of isospora suis were carried out in germany, austria and switzerland including a questionnaire regarding herd size, health status and management practices and a coccidiosis sampling kit for pooled faecal samples from litters of suckling piglets. a total of 184 veterinary practices participated in the survey and returned 1745 samples (331 kits) from 324 farms in the north (n = 98), south (n = 84), centre/east (n = 42) and west (n = 10) of germany, austria (n ... | 2005 | 15752269 |
| effects of toltrazuril on the growth of piglets in herds without clinical isosporosis. | isospora suis is a widely prevalent and economically important parasite. the antiprotozoal compound, toltrazuril, was given as a single treatment to piglets without clinical signs in 10 herds. the daily weight gain (dwg) and mortality between the treatment date and weaning was compared on each herd in 10 treated and 10 control litters. the faeces of control litters were examined for oocyst excretion. in six herds, no oocysts were detected and treatment had no effect on dwg or mortality. in four ... | 2007 | 16122955 |
| effects of toltrazuril on the growth of piglets in herds without clinical isosporosis. | isospora suis is a widely prevalent and economically important parasite. the antiprotozoal compound, toltrazuril, was given as a single treatment to piglets without clinical signs in 10 herds. the daily weight gain (dwg) and mortality between the treatment date and weaning was compared on each herd in 10 treated and 10 control litters. the faeces of control litters were examined for oocyst excretion. in six herds, no oocysts were detected and treatment had no effect on dwg or mortality. in four ... | 2007 | 16122955 |
| isospora suis: an experimental model for mammalian intestinal coccidiosis. | piglets experimentally infected with 10,000 oocysts of isospora suis in three identical trials (n = 50) were examined clinically and coproscopically from 5 to 11 days post-infection (d.p.i.), weighed in weekly intervals until the fourth week of life and compared to age-matched asymptomatic controls (n = 17). furthermore, 17 infected piglets were histologically examined on days 5-14 p.i. infected animals had a significantly lower weight gain than the controls and showed diarrhoea throughout, with ... | 2006 | 16323027 |
| isospora suis: an experimental model for mammalian intestinal coccidiosis. | piglets experimentally infected with 10,000 oocysts of isospora suis in three identical trials (n = 50) were examined clinically and coproscopically from 5 to 11 days post-infection (d.p.i.), weighed in weekly intervals until the fourth week of life and compared to age-matched asymptomatic controls (n = 17). furthermore, 17 infected piglets were histologically examined on days 5-14 p.i. infected animals had a significantly lower weight gain than the controls and showed diarrhoea throughout, with ... | 2006 | 16323027 |
| [comparison of different methods for examining the feces of suckling piglets for isospora suis]. | a highly sensitive diagnostic method is of great importance for detection of i. suis (isospora suis). in the present study ksfv (combined sedimentation-flotation method) using different flotation solutions (nacl, znso4, nacl-sucrose) was investigated regarding sensitivity, costs and expenditure of human labour in comparison to fv (flotation method) using nacl-sucrose solution and to am (autofluorescence microscopy). ninety faecal samples were examined. using am, oocysts were detected in 83.3% of ... | 2006 | 17009709 |
| [comparison of different methods for examining the feces of suckling piglets for isospora suis]. | a highly sensitive diagnostic method is of great importance for detection of i. suis (isospora suis). in the present study ksfv (combined sedimentation-flotation method) using different flotation solutions (nacl, znso4, nacl-sucrose) was investigated regarding sensitivity, costs and expenditure of human labour in comparison to fv (flotation method) using nacl-sucrose solution and to am (autofluorescence microscopy). ninety faecal samples were examined. using am, oocysts were detected in 83.3% of ... | 2006 | 17009709 |
| efficacy of various anticoccidials against experimental porcine neonatal isosporosis. | the efficacies of 20 mg/kg body weight (bw) of toltrazuril (toltra) 2 days post-infection (dpi), 2 mg/kg bw of diclazuril 2 and 3 dpi and 200 mg/kg bw of sulphadimidine 2, 3 and 4 dpi were compared in a model for piglet isosporosis. weight gain (first 4 weeks of life) and diarrhoea and oocyst excretion from 4 to 11 dpi were evaluated (10-12 piglets/group). additionally, animals were killed and examined for pathohistological changes of the small intestines 5, 7, 11 and 14 dpi (n = 3 per group and ... | 2007 | 17048000 |
| efficacy of various anticoccidials against experimental porcine neonatal isosporosis. | the efficacies of 20 mg/kg body weight (bw) of toltrazuril (toltra) 2 days post-infection (dpi), 2 mg/kg bw of diclazuril 2 and 3 dpi and 200 mg/kg bw of sulphadimidine 2, 3 and 4 dpi were compared in a model for piglet isosporosis. weight gain (first 4 weeks of life) and diarrhoea and oocyst excretion from 4 to 11 dpi were evaluated (10-12 piglets/group). additionally, animals were killed and examined for pathohistological changes of the small intestines 5, 7, 11 and 14 dpi (n = 3 per group and ... | 2007 | 17048000 |
| control of piglet coccidiosis by chemical disinfection with a cresol-based product (neopredisan 135-1). | isospora suis is a common pathogen in piggeries and one of the main causative agents of scours in suckling piglets. besides specific treatment, optimised hygiene including chemical disinfection is considered essential in the control of isosporosis. the suitability of the cresol-based product neopredisan 135-1(r) (np) to inactivate oocysts in vitro and to reduce infection pressure in commercial piggeries was evaluated. under in vitro conditions, np at a final concentration of 2 or 4% induced lysi ... | 2007 | 17364163 |
| control of piglet coccidiosis by chemical disinfection with a cresol-based product (neopredisan 135-1). | isospora suis is a common pathogen in piggeries and one of the main causative agents of scours in suckling piglets. besides specific treatment, optimised hygiene including chemical disinfection is considered essential in the control of isosporosis. the suitability of the cresol-based product neopredisan 135-1(r) (np) to inactivate oocysts in vitro and to reduce infection pressure in commercial piggeries was evaluated. under in vitro conditions, np at a final concentration of 2 or 4% induced lysi ... | 2007 | 17364163 |