developmentally regulated sesquiterpene production confers resistance to colletotrichum gloeosporioides in ripe pepper fruits.sesquiterpenoid capsidiol, exhibiting antifungal activity against pathogenic fungus, is accumulated in infected ripe pepper fruits. in this study, we found a negative relation between the capsidiol level and lesion size in fruits infected with colletotrichum gloeosporioides, depending on the stage of ripening. to understand the developmental regulation of capsidiol biosynthesis, fungal-induced gene expressions in the isoprenoid biosynthetic pathways were examined in unripe and ripe pepper fruits ...201425286411
application of volatile antifungal plant essential oils for controlling pepper fruit anthracnose by colletotrichum gloeosporioides.anthracnose caused by colletotrichum gloeosporioides has been destructive during pepper fruit production in outdoor fields in korea. in vitro antifungal activities of 15 different plant essential oils or its components were evaluated during conidial germination and mycelial growth of c. gloeosporioides. in vitro conidial germination was most drastically inhibited by vapour treatments with carvacrol, cinnamon oil, trans-cinnamaldehyde, citral, p-cymene and linalool. inhibition of the mycelial gro ...201526361475
control of colletotrichum gloeosporioides (penz.) sacc. in yellow passion fruit using cymbopogon citratus essential oil.the use of antibiotics in agriculture is limited when compared to their applications in human and veterinary medicine. on the other hand, the use of antimicrobials in agriculture contributes to the drug resistance of human pathogens and has stimulated the search for new antibiotics from natural products. essential oils have been shown to exert several biological activities including antibacterial and antifungal actions. the aim of this study was to determine the activity of 28 essential oils fro ...201024031465
an aboveground pathogen inhibits belowground rhizobia and arbuscular mycorrhizal fungi in phaseolus vulgaris.induced aboveground plant defenses against pathogens can have negative effects on belowground microbial symbionts. while a considerable number of studies have utilized chemical elicitors to experimentally induce such defenses, there is surprisingly little evidence that actual aboveground pathogens affect root-associated microbes. we report here that an aboveground fungal pathogen of common bean (phaseolus vulgaris) induces a defense response that inhibits both the belowground formation of root n ...201425429887
the endochitinase chia btt of bacillus thuringiensis subsp. tenebrionis dsm-2803 and its potential use to control the phytopathogen colletotrichum gloeosporioides.bacillus thuringiensis subsp. tenebrionis dsm-2803 has been studied extensively and spore/crystal mixtures of this strain are used widely in commercial products to control coleopteran pests. the endochitinase chia btt gene of b. thuringiensis subsp. tenebrionis dsm-2803 was cloned and expressed in escherichia coli. the recombinant 6x-histidine tagged protein (rchia btt, ~74 kda), was purified by a hitrap ni affinity column. the km of rchia btt was 0.847 μmol l(-1) and its optimal activity occurr ...201627173732
genetic transformation with the gfp gene of colletotrichum gloeosporioides isolates from coffee with blister spot.blister spot (colletotrichum gloeosporioides) is now widespread in most coffee producing states of brazil, becoming a limiting factor for production. the lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. monitoring of genetically modified organisms has proven to be an effective tool in understanding the host × pathogen interactions. thus, the present s ...201224031947
essential oil prepared from cymbopogon citrates exerted an antimicrobial activity against plant pathogenic and medical microorganisms.essential oils are mixtures of volatile, lipophilic compounds originating from plants. some essential oils have useful biological activities including antimicrobial, spasmolytic, antiplasmodial, and insect-repelling activities. in this study, we tested the antimicrobial activity of essential oil prepared from the aromatic plant, cymbopogon citrates, against three important plant pathogenic and medical microorganisms, pectobacterium carotovorum, colletotrichum gloeosporioides, and aspergillus nig ...200923983507
physcomitrella patens activates defense responses against the pathogen colletotrichum gloeosporioides.the moss physcomitrella patens is a suitable model plant to analyze the activation of defense mechanisms after pathogen assault. in this study, we show that colletotrichum gloeosporioides isolated from symptomatic citrus fruit infects p. patens and cause disease symptoms evidenced by browning and maceration of tissues. after c. gloeosporioides infection, p. patens reinforces the cell wall by the incorporation of phenolic compounds and induces the expression of a dirigent-protein-like encoding ge ...201526389888
antibacterial and antifungal activities of stereum ostrea, an inedible wild mushroom.antibacterial and antifungal activities of liquid culture filtrate, water and ethanol extract (solid culture) of stereum ostrea were evaluated against 5 bacteria and 3 plant pathogenic fungi. to determine the minimal inhibitory concentration (mic), we studied 5~300 mg/ml concentrations against bacteria and fungi separately. the mic was 10 mg/ml for bacillus subtilis and 40 mg/ml for colletotrichum gloeosporioides and colletotrichum miyabeanus. liquid culture filtrate was more effective against g ...200724015099
first total syntheses and antimicrobial evaluation of penicimonoterpene, a marine-derived monoterpenoid, and its various derivatives.the first total synthesis of marine-derived penicimonoterpene (±)-1 has been achieved in four steps from 6-methylhept-5-en-2-one using a reformatsky reaction as the key step to construct the basic carbon skeleton. a total of 24 new derivatives of 1 have also been designed and synthesized. their structures were characterized by analysis of their 1h nmr, 13c nmr and hresims data. some of them showed significant antibacterial activity against aeromonas hydrophila, escherichia coli, micrococcus lute ...201424897384
identification of conidiogenesis-associated genes in colletotrichum gloeosporioides by agrobacterium tumefaciens-mediated transformation.the colletotrichum gloeosporioides is one of the most significant pathogens leading to huge economic losses. to infect plants and cause disease dissemination, the fungus elaborates to produce asexual spores called conidia, which are long-lived and highly resistant to environmental stresses. here, we report a large-scale, systematic genome-wide screening of conidiogenesis-associated genes via conidiation assays, and high-efficiency tail-pcrs. of 10,210 independent transformants tested, 59 mutants ...201627582094
genetic transformation of extremophilic fungi acidea extrema and acidothrix acidophila.intact, growing cells of strongly acidophilic fungi acidea extrema and acidothrix acidophila have been successfully transformed by introduction of heterologous dna fragment (composed of the glyceraldehyde-phosphate-dehydrogenase gene promoter from emericella nidulans, a metallothionein-coding gene asmt1 from amanita strobiliformis and glyceraldehyde-phosphate-dehydrogenase gene terminator from colletotrichum gloeosporioides) with the length of 1690 bp. the transformation procedure was based on t ...201525934267
cloning of insertion site flanking sequence and construction of transfer dna insert mutant library in stylosanthes colletotrichum.stylosanthes sp. is the most important forage legume in tropical areas worldwide. stylosanthes anthracnose, which is mainly caused by colletotrichum gloeosporioides, is a globally severe disease in stylo production. little progress has been made in anthracnose molecular pathogenesis research. in this study, agrobacterium tumefaciens-mediated transformation was used to transform stylosanthes colletotrichum strain ch008. the major factors of the genetic transformation system of s. colletotrichum w ...201425361073
identification of virulence genes in the crucifer anthracnose fungus colletotrichum higginsianum by insertional investigate the molecular and genetic mechanisms underlying virulence of colletotrichum higginsianum on arabidopsis thaliana, a t-dna insertion mutant library of c. higginsianum, the causal agent of crucifer anthracnose, was established using agrobacterium tumefaciens-mediated transformation. among 875 transformants tested for virulence on arabidopsis, six mutants with altered virulence, including an appressorial melanin-deficient mutant t734, two mutants defective in penetration, t45 and b30 ...201323806215
identifying pathogenicity genes in the rubber tree anthracnose fungus colletotrichum gloeosporioides through random insertional gain more insight into the molecular mechanisms of colletotrichum gloeosporioides pathogenesis, agrobacterium tumefaciens-mediated transformation (atmt) was used to identify mutants of c. gloeosporioides impaired in pathogenicity. an atmt library of 4128 c. gloeosporioides transformants was generated. transformants were screened for defects in pathogenicity with a detached copper brown leaf assay. 32 mutants showing reproducible pathogenicity defects were obtained. southern blot analysis show ...201323602122
characterization of alcaligenes faecalis strain ad15 indicating biocontrol activity against plant pathogens.bacterial strain possessing both bacteriostatic and fungistatic activity (biocontrol activity) against pathogens of cyclamen (cyclamen sp.) was isolated from the soil in gifu prefecture, japan, and characterized with respect to its taxonomic and biocontrol properties. the sequence of its 16s rrna gene, morphology, biochemistry, and fatty acid composition demonstrated that it is a strain most closely related to alcaligenes faecalis subsp. faecalis lmg 1229(t). the isolate was named a. faecalis st ...201323759862
triterpenoids from the herbs of salicornia bigelovii.a new nortriterpene saponin, 3-o-β-d-glucuronopyranosyl-30-norolean-12,20(29)-dien-23- oxo-28-oic acid, namely bigelovii d (11), was isolated from the hydroalcoholic extract of herbs of salicornia bigelovii along with 10 known saponins (1-10). their chemical structures were identified on the basis of spectroscopic analyses including two-dimensional nmr and a comparison with literature data. some of these compounds showed potent antifungal activities in vitro. compounds 3, 4, 5, 6, 7, 10 and 11 d ...201526569214
fungistatic activity of zanthoxylum rhoifolium lam. bark extracts against fungal plant pathogens and investigation on mechanism of action in botrytis cinerea.plant-derived compounds are emerging as an alternative choice to synthetic fungicides. chloroform-methanol extract, obtained from the bark of zanthoxylum rhoifolium, a member of rutaceae, showed a fungistatic effect on botrytis cinerea, sclerotinia sclerotiorum, alternaria alternata, colletotrichum gloeosporioides and clonostachys rosea, when added to the growth medium at different concentrations. a fraction obtained by gel separation and containing the alkaloid o-methylcapaurine showed signific ...201525589008
[diversity and community structure of endophytic fungi from taxus chinensis var. mairei].a total of 628 endophytic fungi were isolated from 480 tissue segments of needles and branches of taxus chinensis var. mairei. according to morphological characteristics and its sequences, they represented 43 taxa in 28 genera, of which 10 hyphomycetes, 20 coelomycetes, 12 ascomycetes and 1 unknown fungus. phomopsis mali was confirmed as the dominant species. in accordance with relative frequency, alternaria alternata, aureobasidium pullulans, colletotrichum boninense, c. gloeosporioides, epicoc ...201425345060
[species and ecological control of disease on cultivated gentiana rigescens in yunnan].to find out variety of the fungal diseases of cultivated gentiana rigescens and provide important basis for prevention.201222734403
metabolites from aspergillus fumigatus, an endophytic fungus associated with melia azedarach, and their antifungal, antifeedant, and toxic activities.thirty-nine fungal metabolites 1-39, including two new alkaloids, 12β-hydroxy-13α-methoxyverruculogen tr-2 (6) and 3-hydroxyfumiquinazoline a (16), were isolated from the fermentation broth of aspergillus fumigatus ln-4, an endophytic fungus isolated from the stem bark of melia azedarach. their structures were elucidated on the basis of detailed spectroscopic analysis (mass spectrometry and one- and two-dimensional nmr experiments) and by comparison of their nmr data with those reported in the l ...201222409377
[phaeohyphomycosis caused by colletotrichum gloeosporioides and alternaría infectoria in renal transplant recipient].several species of black fungi have been reported as agents of subcutaneous phaeohyphomycosis. although most of these fungi are considered opportunistic pathogens, they play an important role in phaeohyphomycosis, a disease considered an emergent mycosis among solid organ recipients. we report a case of phaeohyphomycosis caused by alternaria infectoria of the left hand and the 4th finger of the right hand of a 68-year-old male who underwent a renal transplant 35 months before. the lesion was tre ...201425327202
identification and characterization of an antifungal protein, afafpr9, produced by marine-derived aspergillus fumigatus r9.a fungal strain, r9, was isolated from the south atlantic sediment sample and identified as aspergillus fumigatus. an antifungal protein, afafpr9, was purified from the culture supernatant of aspergillus fumigatus r9. afafpr9 was identified to be restrictocin, which is a member of the ribosome-inactivating proteins (rips), by maldi-tof-tof-ms. afafpr9 displayed antifungal activity against plant pathogenic fusarium oxysporum, alternaria longipes, colletotrichum gloeosporioides, paecilomyces vario ...201525394604
seedborne pathogenic fungi in common bean (phaseolus vulgaris cv. inta rojo) in nicaragua.common bean (phaseolus vulgaris l.) is an important legume with high nutritional value. in nicaragua, certified healthy seeds of local bean varieties are not available, and seedborne fungi have gained little attention. here, were surveyed seedborne pathogenic fungi in an important local bean cultivar, 'inta rojo'. beans grown in the four main production areas in nicaragua (boaco, carazo, estelí, matagalpa) for future use as seed stock were sampled from four seed storehouses and six seed lots. a ...201627997595
characterization of the bioactive metabolites from a plant growth-promoting rhizobacteria and their exploitation as antimicrobial and plant growth-promoting agents.a plant growth-promoting bacterial strain, pm 105, isolated from a tea plantation soil from the north eastern region of india was identified as pseudomonas aeruginosa through classical and 16s ribosomal dna (rdna) gene sequencing. further studies with this strain confirmed broad spectrum antifungal activity against ten human and plant pathogenic fungal pathogens viz. aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus tubingensis, candida albicans, colletotrichum gloeospori ...201525832181
antifungal activity of lactobacilli and its relationship with 3-phenyllactic acid this study, 13 lactic acid bacteria (lab) strains (including 5 lactobacillus casei, 2 lactobacillus rhamnosus, 2 lactobacillus fermentum, 1 lactobacillus acidophilus, 1 lactobacillus plantarum, 1 lactobacillus sakei, and 1 lactobacillus reuteri species) were assessed for both their antifungal activity against four food spoilage molds (colletotrichum gloeosporioides, botrytis cinerea, penicillium expansum, and aspergillus flavus) and their capability to produce the novel antimicrobial compound ...201424412414
cation-stress-responsive transcription factors slta and crza regulate morphogenetic processes and pathogenicity of colletotrichum gloeosporioides.growth of colletotrichum gloeosporioides in the presence of cation salts nacl and kcl inhibited fungal growth and anthracnose symptom of colonization. previous reports indicate that adaptation of aspergillus nidulans to salt- and osmotic-stress conditions revealed the role of zinc-finger transcription factors slta and crza in cation homeostasis. homologs of a. nidulans slta and crza were identified in c. gloeosporioides. the c. gloeosporioides crza homolog is a 682-amino acid protein, which cont ...201628030573
carbon regulation of environmental ph by secreted small molecules that modulate pathogenicity in phytopathogenic fungi.fruit pathogens can contribute to the acidification or alkalinization of the host environment. this capability has been used to divide fungal pathogens into acidifying and/or alkalinizing classes. here, we show that diverse classes of fungal pathogens-colletotrichum gloeosporioides, penicillium expansum, aspergillus nidulans and fusarium oxysporum-secrete small ph-affecting molecules. these molecules modify the environmental ph, which dictates acidic or alkaline colonizing strategies, and induce ...201626666972
in vitro photodynamic inactivation of plant-pathogenic fungi colletotrichum acutatum and colletotrichum gloeosporioides with novel phenothiazinium photosensitizers.the increasing tolerance to currently used fungicides in both clinical and agricultural areas is of great concern. the nonconventional light-based approach of antimicrobial photodynamic treatment (apdt) is a promising alternative to conventional fungicides. we evaluated the effects of apdt with four phenothiazinium derivatives (methylene blue [mb], new methylene blue n [nmbn], toluidine blue o [tbo], and the novel pentacyclic phenothiazinium photosensitizer [ps] s137) on conidia of three fungal ...201424362436
biological control of toxigenic citrus and papaya-rotting fungi by streptomyces violascens mt7 and its extracellular indian indigenous, loktak lake soil isolate streptomyces violascens mt7 was assessed for its biocontrol potential both in vitro and in vivo against toxigenic fruit-rotting fungi. strain mt7 exhibited broad-spectrum antifungal activity against various pathogenic postharvest fungi of citrus and papaya. in shake-flask fermentation, antagonist s. violascens mt7 highly produced extracellular antifungal metabolites in early stationary growth phase in glucose-yeast extract-malt extract (m93) broth. ...201526214840
a novel glucose dehydrogenase from the white-rot fungus pycnoporus cinnabarinus: production in aspergillus niger and physicochemical characterization of the recombinant on glucose dehydrogenases (gdhs) are scarce and availability of these enzymes for application purposes is limited. this paper describes a new gdh from the fungus pycnoporus cinnabarinus cirm brfm 137 that is the first reported gdh from a white-rot fungus belonging to the basidiomycota. the enzyme was recombinantly produced in aspergillus niger, a well-known fungal host producing an array of homologous or heterologous enzymes for industrial applications. the full-length gene that encodes gdh ...201424965558
antifungal activity and cytotoxicity of isolated compounds from leaves of breonadia salicina.breonadia salicina is used traditionally to treat wounds, ulcers, fevers, headaches, and fungal infections. the aim of this study was to investigate the antifungal activity of the plant extract and compounds isolated there from.201323727185
identification of antifungal niphimycin from streptomyces sp. kp6107 by screening based on adenylate kinase assay.microbial culture extracts are used for natural product screening to find antifungal lead compounds. a microbial culture extract library was constructed using 343 actinomycete isolates to examine the value of the adenylate kinase (ak) assay for screening to identify antifungal metabolites that disrupt cell integrity in plant pathogenic fungi. a culture extract of streptomyces sp. strain kp6107 lysed cells of fusarium oxysporum f.sp. lycopersici which resulted in high ak activity. the active ingr ...201322915202
bacillus species (bt42) isolated from coffea arabica l. rhizosphere antagonizes colletotrichum gloeosporioides and fusarium oxysporum and also exhibits multiple plant growth promoting activity.colletotrichum and fusarium species are among pathogenic fungi widely affecting coffea arabica l., resulting in major yield loss. in the present study, we aimed to isolate bacteria from root rhizosphere of the same plant that is capable of antagonizing colletotrichum gloeosporioides and fusarium oxysporum as well as promotes plant growth.201627863465
are cyclic lipopeptides produced by bacillus amyloliquefaciens s13-3 responsible for the plant defence response in strawberry against colletotrichum gloeosporioides?the antagonistic strain bacillus amyloliquefaciens strain s13-3 decreased the severity of strawberry anthracnose caused by colletotrichum gloeosporioides. the foliar application of s13-3 triggered the expression of pathogenesis-related proteins, chitinase and β-1,3-glucanase, in strawberry leaves. we identified lipopeptide antibiotics, including iturin a, fengycin, mixirin, pumilacidin and surfactin, produced and secreted by s13-3. iturin a and surfactin elicited the gene expression of the patho ...201525511625
activity of flavanones isolated from rhododendron hainanense against plant pathogenic a search for naturally occurring antimicrobial compounds in medicinal plants and herbs, seven flavanones were isolated from the aerial parts of rhododendron hainanense and were tested for their antimicrobial activities against six bacteria and six plant pathogenic fungi. within the series of flavanones tested, farrerol (1) displayed moderate antibacterial activities against bacillus cereus, b. subtilis, pseudomonas aeruginosa, staphylococcus aureus, escherichia coli and erwinia carotovora, wi ...201627319130
impact of bacillus cereus nrkt on grape ripe rot disease through resveratrol synthesis in berry skin.vine growers are faced with the difficult problem of how to control grape ripe rot disease in vineyards because of fear of accumulation of pesticide residues on grape berries near harvest. biological control is an alternative non-hazardous technique to control the diseases.201727038426
antimicrobial compounds from endophytic streptomyces sp. bcc72023 isolated from rice (oryza sativa l.).an endophytic actinomycete strain bcc72023 was isolated from rice (oryza sativa l.) and identified as the genus streptomyces, based on phenotypic, chemotaxonomic and 16s rrna gene sequence analyses. the strain showed 99.80% similarity compared with streptomyces samsunensis m1463(t). chemical investigation led to the isolation of three macrolides, efomycins m (1), g (2) and oxohygrolidin (3), along with two polyethers, abierixin (4) and 29-o-methylabierixin (5). to our knowledge, this is the firs ...201626809052
antimicrobial and allelopathic metabolites produced by penicillium brasilianum.six known compounds, isoroquefortine c (1), griseofulvin (2), ergosterol peroxide (3), 3β-hydroxy-(22e,24r)-ergosta-5,8,22-trien-7-one (4), cerevisterol (5) and (22e,24r)-6β-methoxyergosta-7,22-diene-3β,5α-diol (6), were produced by the fungus penicillium brasilianum, and their structures were elucidated by spectroscopic methods. this is the first report on isoroquefortine c as naturally occurring compound. their bioactivities against five phytopathogenic fungi (gibeberalla saubinetti, fusarium ...201525103127
antifungal and antibacterial metabolites from an endophytic aspergillus sp. associated with melia known metabolites, dianhydro-aurasperone c (1), isoaurasperone a (2), fonsecinone a (3), asperpyrone a (4), asperazine (5), rubrofusarin b (6) and (r)-3-hydroxybutanonitrile (7), were isolated from the culture of aspergillus sp. kj-9, a fungal endophyte isolated from melia azedarach and identified by spectroscopic methods. all isolates were evaluated in vitro against several phytopathogenic fungi (gibberella saubinetti, magnaporthe grisea, botrytis cinerea, colletotrichum gloeosporioides a ...201424708541
trans-cinnamic acid and xenorhabdus szentirmaii metabolites synergize the potency of some commercial fungicides.development of novel approaches for the control of fungal phytopathogens is desirable. in this study we hypothesized that the combination of commercial fungicides with certain enhancing agents could result in synergistic levels of control. prior research has indicated that trans-cinnamic-acid (tca), a metabolite of the bacteria photorhabdus luminescens and metabolites of xenorhabdus szentirmaii are particularly toxic to various phytpathogenic fungi when compared to metabolites of other xenorhabd ...201728322849
identification of lipopeptides produced by bacillus subtilis czk1 isolated from the aerial roots of rubber trees.we obtained a strain of bacillus subtilis, which we named czk1, from the aerial roots of rubber trees. this bacterial isolate exhibits strong antagonistic activity against ganoderma pseudoferreum, phellinus noxius, helicobasidium compactum, rigidoporus lignosus, sphaerostilbe repens, and colletotrichum gloeosporioides. our earlier research has shown that the antagonistic activity of a fermentation supernatant czk1 isolate produces a complex mixture of lipopeptides. in this study, we used methano ...201728252162
effects of rhizobacteria paenibacillus polymyxa apec136 and >bacillus subtilis apec170 on biocontrol of postharvest pathogens of apple this study, plant growth-promoting rhizobacteria (pgpr) were evaluated as potential biocontrol agents against postharvest pathogens of apple fruits. in vitro bioassays revealed that, out of 30 isolates screened, isolates apec136 and apec170 had the most significant inhibitory effects against the mycelial growth of several fungal pathogens. analysis of 16s ribosomal rna (rrna) sequences identified the two effective isolates as paenibacillus polymyxa and bacillus subtilis, respectively. the two ...201727921398
a new macrolactin antibiotic from deep sea-derived bacteria bacillus subtilis b5.a new macrolactin derivate, 7-o-2'e-butenoyl macrolactin a (1), together with three known macrolactin compounds, macrolactin a (2), 7-o-malonyl macrolactin a (3) and 7-o-succinyl macrolactin a (4), was isolated from the bacterial strain bacillus subtilis b5, which was isolated from the 3000 m deep sea sediment of the southwest pacific ocean. the structures of the new compounds were assigned by spectroscopic methods including 1-d/2-d nmr and ms analysis techniques. compounds 1 and 2 demonstrated ...201627071303
partial purification, characterization, and kinetic studies of a low-molecular-weight, alkali-tolerant chitinase enzyme from bacillus subtilis jn032305, a potential biocontrol strain.a new alkalophilic low-molecular-mass chitinase of 14 kd from the potent biocontrol agent bacillus subtilis jn032305 was partially purified and enzymology of the chitinase was studied. the enzyme showed optimal ph of 9.0 and temperature of 50°c. the enzyme was found stable during the 60-min incubation at 50 °c. the chitinase was inhibited by group specific agents like iaa, dan, tlck, and sds and metal ions mg(2+), ca(2+), fe(2+), mn(2+), ba(2+), and hg(2+), whereas zn(2+) did not show significan ...201424499366
potentiality of bacillus subtilis as biocontrol agent for management of anthracnose disease of chilli caused by colletotrichum gloeosporioides ogc1.a soil bacterium, bacillus subtilis, isolated from the rhizosphere of chilli, showed high antagonistic activity against colletotrichum gloeosporioides ogc1. a clear inhibition zone of 0.5-1 cm was observed in dual plate assay. microscopic observations showed a clear hyphal lysis and degradation of fungal cell wall. in dual liquid cultures, the b. subtilis strain inhibited the c. gloeosporioides up to 100 % in terms of dry weight. this strain also produced a clear halo region on chitin agar mediu ...201428324440
a novel antifungal protein of bacillus subtilis b25.bacillus subtilis b25 was isolated from banana rhizosphere soil. it has been confirmed for b25 to have stronger antagonism against fusarium oxysporum f.sp.cubense, additionally b25 has good inhibitory to plant pathogens, including corynespora cassiicola, alternaria solani, botrytis cinerea and colletotrichum gloeosporioides on potato dextrose agar (pda) plates. the antagonistic substance can be extracted from cell-free culture broth supernatants by 70% (w/v) (nh4)2 so4 saturation. clear blank ba ...201324255843
identification of citrinin as the defence metabolite of penicillium corylophilum stressed with the antagonist fungus beauveria bassiana.penicillium corylophilum isolated from mosquitoes was cultivated in liquid media leading to the first reported identification of citrinin (1a) as one metabolic component of this fungus. the produced amount of 1a indicated this compound as the most abundant secondary metabolite of this fungus. stressing the culture of p. corylophilum with the presence of the antagonistic fungus beauveria bassiana increased in 65% the production of 1a. similar results were obtained with the presence of other fungi ...201222414191
a pr-4 gene identified from malus domestica is involved in the defense responses against botryosphaeria dothidea.pathogenesis-related protein-4 (pr-4) family is a group of proteins with a barwin domain in c-terminus and generally thought to be involved in plant defense responses. however, their detailed roles are poorly understood in defense of apple plant against pathogenic infection. in the present study, a new pr-4 gene (designated as mdpr-4) was identified from malus domestica, and its roles in defense responses of apple were investigated. the open reading frame of mdpr-4 gene is of 447 bp encoding a p ...201323178481
synthesis and biological evaluation of arylpyrazoles as fungicides against phytopathogenic fungi.3-phenol-1h-pyrazoles (2), 4-halogeno-3-phenol-1h-pyrazoles (3) and 2-(1-phenol-1h-pyrazol-5-yl)phenols (4) were prepared by the condensation of (e)-3-(dimethylamino)-1-phenylprop-2-en-1-ones and hydrazine hydrate or phenylhydrazine in good yields. they were evaluated against five phytopathogens fungi, namely cytospora sp., colletotrichum gloeosporioides, botrytis cinerea, alternaria solani and fusarium solani in vitro. most of the above-mentioned compounds exhibited activities. for example, 4-c ...201728247170
synthesis of pyrazolo[1,5-a]pyrimidine derivatives and their antifungal activities against phytopathogenic fungi in vitro.5,6-diarylpyrazolo[1,5-a]pyrimidines (3) and 6,7-diarylpyrazolo[1,5-a]pyrimidines (4) were chemoselectively synthesized by the condensation of isoflavone (1) and 3-aminopyrazole (2). 5,6-diarylpyrazolo[1,5-a]pyrimidines (3) were obtained via microwave irradiation, and 6,7-diarylpyrazolo[1,5-a]pyrimidines (4) were obtained via conventional heating. in addition, the pyrimidine derivatives 3 and 4 were evaluated against five phytopathogenic fungi (cytospora sp., colletotrichum gloeosporioides, botr ...201627599494
assessment of injuries caused by anastrepha fraterculus (wied.) (diptera: tephritidae) on the incidence of bunch rot diseases in table grape.anastrepha fraterculus (wied.) is the main insect pest of table grapes (vitis vinifera) in the southern region of brazil. in this study, we aimed to investigate the effect of fruit puncturing by adult females and larval infestation by a. fraterculus on the occurrence of bunch rot disease in the grape (cultivar "itália") by evaluating grapes (a) punctured for oviposition by females of a. fraterculus, sterilized in laboratory with novaluron (40 mg l(-1)) and further spray-inoculated separately wit ...201626911161
experimental protocol for the recovery and evaluation of bioactive compounds of tarbush against postharvest fruit fungi.the aim of this study was to recover and evaluate in vitro the antifungal activity of bioactive compounds of tarbush flourensia cernua against fruit postharvest fungi and their antioxidant capacity. a yield of 15% of bioactive compounds of tarbush was obtained by infusion method and heating using water as solvent. a concentration of 4000 mg/l showed a higher antioxidant activity against the abts radical (3.21 μmol/g) in comparison with the dpph radical (7.62 μmol/g); however the dpph radical sho ...201626769505
antifungal substances from streptomyces sp. a3265 antagonistic to plant pathogenic a previous study, we identified a streptomyces sp., a3265, as exhibiting potent antifungal activity against various plant pathogenic fungi, including botrytis cinerea, colletotrichum gloeosporioides, and rhizoctonia solani. this strain also exhibited a biocontrolling effect against ginseng root rot and damping-off disease, common diseases of ginseng and other crops. in this study, we isolated two antifungal substances responsible for this biocontrolling effect via diaion hp-20 and sephadex lh ...201526539051
synthesis of heterocycle-attached methylidenebenzenesulfonohydrazones as antifungal agents.a series of heterocycle-attached methylidenebenzenesulfonohydrazone derivatives were synthesized and evaluated for their antifungal activities against seven phytopathogenic fungi such as fusarium graminearum, alternaria solani, valsa mali, phytophthora capsici, fusarium solani, botrytis cinerea, and glomerella cingulata. compounds 7b, 8d, 9a, 9b and 9d exhibited a good and broad-spectrum of antifungal activities against at least five phytopathogenic fungi at the concentration of 100 μg/ml. it de ...201526471091
nor-mevaldic acid surrogates as selective antifungal agent leads against botrytis cinerea. enantioselective preparation of 4-hydroxy-6-(1-phenylethoxy)tetrahydro-2h-pyran-2-one.solvent-free desymmetrisation of meso-dialdehyde 1 with chiral 1-phenylethan-1-ol, led to preparation of 4-silyloxy-6-alkyloxytetrahydro-2h-pyran-2-one (+)-3a with a 96:4 dr deprotected lactone (+)-19a and the related racemic lactones 16a-18a present a lactone moiety resembling the natural substrate of hmg-coa reductase and their antifungal properties have been evaluated against the phytopathogenic fungi botrytis cinerea and colletotrichum gloeosporioides. these compounds were selectively active ...201525971873
bioactive metabolites from the mycelia of the basidiomycete hericium known compounds, three diketopiperazine alkaloids, 12β-hydroxyverruculogen tr-2 (1), fumitremorgin c (2) and methylthiogliotoxin (5), two hetero-spirocyclic γ-lactam alkaloids, pseurotin a (3) and fd-838 (4), and cerevisterol (6) and herierin iv (7), were isolated from the mycelia of the basidiomycete hericium erinaceum and identified by spectroscopic analyses. the antioxidant and antifungal activities of compounds 1-6 were evaluated. the results indicated that compounds 1, 3 and 6 exhibit ...201424635196
methyl anthranilate and γ-decalactone inhibit strawberry pathogen growth and achene germination.plant volatile compounds have been shown to affect microbial growth and seed germination. here two fruity volatiles found in strawberry ( fragaria × ananassa ), γ-decalactone ("peachlike" aroma) and methyl anthranilate ("grapelike" aroma), were tested for effects on relevant pathogens and seedling emergence. significant growth reduction was observed for botrytis cinerea , colletotrichum gloeosporioides , colletotrichum acutatum , phomopsis obscurans , and gnomonia fragariae at 1 mm γ-decalactone ...201324328200
antifungal activity of diketopiperazines and stilbenes against plant pathogenic fungi in vitro.the present study aimed to investigate antifungal activity of a stilbene and diketopiperazine compounds against plant pathogenic fungi, including phytophthora capsici, p. colocasiae, botrytis cinerea and colletotrichum gloeosporioides. minimal inhibition concentrations (mic) and minimal fungicidal concentrations (mfc) of stilbenes and diketopiperazines for each fungus were determined using microplate method. best activity was recorded by stilbenes against p. capsici and p. colocasiae. all four t ...201424122628
synthesis and antifungal activity of 2-chloromethyl-1h-benzimidazole derivatives against phytopathogenic fungi in vitro.a series of 35 benzimidazole derivatives were synthesized from 2-chloromethyl-1h-benzimidazole in good yields. their structures were characterized by (1)h and (13)c nmr and hresims. antifungal activities of all of the synthesized compounds were evaluated against five phytopathogens fungi (cytospora sp., colletotrichum gloeosporioides, botrytis cinerea, alternaria solani, and fusarium solani) using the mycelium growth rate method. compound 4m displayed strong growth inhibition of c. gloeosporioid ...201323419161
burkholderia cepacia xxvi siderophore with biocontrol capacity against colletotrichum gloeosporioides.colletotrichum gloeosporioides is the causal agent of anthracnose in mango. burkholderia cepacia xxvi, isolated from mango rhizosphere and identified by 16s rdna sequencing as a member of b. cepacia complex, was more effective than 6 other mango rhizosphere bacteria in inhibiting the model mango pathogen, c. gloeosporioides atcc mya 456. biocontrol of this pathogen was demonstrated on petri-dishes containing pda by > 90 % reduction of surface colonization. the nature of the biocontrol metabolite ...201222806187
thailandins a and b, new polyene macrolactone compounds isolated from actinokineospora bangkokensis strain 44ehw(t), possessing antifungal activity against anthracnose fungi and pathogenic yeasts.two new polyene macrolactone antibiotics, thailandins a, 1, and b, 2, were isolated from the fermentation broth of rhizosphere soil-associated actinokineospora bangkokensis strain 44ehw(t). the new compounds from this strain were purified using semipreparative hplc and sephadex lh-20 gel filtration while following an antifungal activity guided fractionation. their structures were elucidated through spectroscopic techniques including uv, hr-esi-ms, and nmr. these compounds demonstrated broad spec ...201627267862
diversity of endophytic fungi in glycine max.endophytic fungi are microorganisms that live within plant tissues without causing disease during part of their life cycle. with the isolation and identification of these fungi, new species are being discovered, and ecological relationships with their hosts have also been studied. in glycine max, limited studies have investigated the isolation and distribution of endophytic fungi throughout leaves and roots. the distribution of these fungi in various plant organs differs in diversity and abundan ...201526111593
antifungal compounds produced by colletotrichum gloeosporioides, an endophytic fungus from michelia this study, eight endophytic fungi were isolated from the leaves, stems and roots of michelia champaca. the isolates were screened and evaluated for their antifungal, anticancer and acetylcholinesterase (ache) inhibitory activities. all of the extracts exhibited potent activity against two evaluated phytopathogenic fungi. chemical investigation of etoac extracts of the endophytic fungus colletotrichum gloeosporioides resulted in the isolation of one new compound, 2-phenylethyl 1h-indol-3-yl-a ...201425421415
antifungal activity against plant pathogens of metabolites from the endophytic fungus cladosporium cladosporioides.bioassay-guided fractionation of cladosporium cladosporioides (fresen.) de vries extracts led to the isolation of four compounds, including cladosporin, 1; isocladosporin, 2; 5'-hydroxyasperentin, 3; and cladosporin-8-methyl ether, 4. an additional compound, 5',6-diacetylcladosporin, 5, was synthesized by acetylation of compound 3. compounds 1-5 were evaluated for antifungal activity against plant pathogens. phomopsis viticola was the most sensitive fungus to the tested compounds. at 30 μm, comp ...201323651409
characterization of an endophytic whorl-forming streptomyces from catharanthus roseus stems producing polyene macrolide endophytic whorl-forming streptomyces sp. designated as ts3ro having antifungal activity against a large number of fungal pathogens, including sclerotinia sclerotiorum, rhizoctonia solani, colletotrichum gloeosporioides, cryphonectria parasitica, fusarium oxysporum, pyrenophora tritici-repentis, epidermophyton floccosum, and trichophyton rubrum, was isolated from surface-sterilized catharanthus roseus stems. preliminary identification showed that streptomyces cinnamoneus subsp. sparsus was it ...201222524528
unveiling members of colletotrichum acutatum species complex causing colletotrichum leaf disease of hevea brasiliensis in sri lanka.colletotrichum is an important fungal genus with great diversity, which causes anthracnose of a variety of crop plants including rubber trees. colletotrichum acutatum and colletotrichum gloeosporioides have been identified as the major causative agents of colletotrichum leaf disease of rubber trees in sri lanka based on morphology, pathogenicity, and the analysis of internally transcribed spacer sequences of the nuclear ribosomal dna. this study has been conducted to investigate the members of t ...201728378161
construction of a system for the strawberry nursery production towards elimination of latent infection of anthracnose fungi by a combination of pcr and microtube of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by colletotrichum acutatum or glomerella cingulata in latent infection stage. we here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, dna extraction, synthesis of pcr-amplified probes and microtube hybridization (mth) using a macroarray. the initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract pcr-inhibitor-f ...201728167891
characterization of a colletotrichum population causing anthracnose disease on olive in northern phenotypically, physiologically and molecularly characterize the causal agent of olive anthracnose in the northern tunisia and to study its genetic variability and pathogenicity.201626876102
efficacy of sdhi fungicides, including benzovindiflupyr, against colletotrichum species.colletotrichum species cause anthracnose diseases on many plants and crops. a new generation of succinate dehydrogenase inhibitors (sdhis) was developed recently. the inhibitory activity of the five sdhi fungicides against colletotrichum species was determined in this study.201626732510
antifungal compounds from turmeric and nutmeg with activity against plant pathogens.the antifungal activity of twenty-two common spices was evaluated against plant pathogens using direct-bioautography coupled colletotrichum bioassays. turmeric, nutmeg, ginger, clove, oregano, cinnamon, anise, fennel, basil, black cumin, and black pepper showed antifungal activity against the plant pathogens colletotrichum acutatum, colletotrichum fragariae, and colletotrichum gloeosporioides. among the active extracts, turmeric and nutmeg were the most active and were chosen for further investi ...201425173461
molecular and phytochemical investigation of angelica dahurica and angelica pubescentis essential oils and their biological activity against aedes aegypti, stephanitis pyrioides, and colletotrichum this study, angelica dahurica and angelica pubescentis root essential oils were investigated as pest management perspectives, and root samples were also analyzed genetically using the nuclear ribosomal internal transcribed spacer (its) region as a dna barcode marker. a. pubescentis root essential oil demonstrated weak antifungal activity against colletotrichum acutatum, colletotrichum fragariae, and colletotrichum gloeosporioides, whereas a. dahurica root essential oil did not show antifungal ...201425133520
chemical composition and biological effects of artemisia maritima and artemisia nilagirica essential oils from wild plants of western himalaya.artemisia species possess pharmacological properties that are used for medical purposes worldwide. in this paper, the essential oils from the aerial parts of artemisia nilagirica and artemisia maritima from the western indian himalaya region are described. the main compounds analyzed by simultaneous gc/ms and gc/fid were camphor and 1,8-cineole from a. maritima, and camphor and artemisia ketone from a. nilagirica. additionally, the oils were evaluated for their antibacterial, antifungal, mosquit ...201425127023
bioassay-directed isolation and identification of phytotoxic and fungitoxic acetylenes from conyza canadensis.conyza canadensis (l.) cronquist syn. (horseweed) is a problematic and invasive weed with reported allelopathic properties. to identify the phytotoxic constituents of the aerial parts, a systematic bioactivity-guided fractionation of the dichloromethane extract was performed. three active enyne derivatives, (2z,8z)-matricaria acid methyl ester, (4z,8z)-matricaria lactone, and (4z)-lachnophyllum lactone, were identified. the lactones inhibited growth of the monocot agrostis stolonifera (bentgrass ...201222612410
occurrence of anthracnose on highbush blueberry caused by colletotrichum species in korea.a total of 82 isolates of colletotrichum species were obtained from anthracnose symptoms of highbush blueberry trees grown in the gochang area of korea during a disease survey in 2008. out of the isolates, 75 were identified as colletotrichum gloeosporioides and the others as c. acutatum based on their morphological and cultural characteristics. twenty six of c. gloeosporioides isolates produced their teleomorph glomerella cingulata in pda culture. three isolates of each c. gloeosporioides and c ...200923983555
effects of biosurfactants, mannosylerythritol lipids, on the hydrophobicity of solid surfaces and infection behaviours of plant pathogenic investigate the effects of mannosylerythritol lipids (mels) on the hydrophobicity of solid surfaces, their suppressive activity against the early infection behaviours of several phytopathogenic fungal conidia, and their suppressive activity against disease occurrences on fungal host plant leaves.201525898775
the function and transcriptome analysis of a bzip transcription factor cgap1 in colletotrichum gloeosporioides.colletotrichum gloeosporioides is an important pathogen of anthracnose, which is able to infect numerous crops in tropical and subtropical regions, causing great economic losses. to investigate the fungal response to host-generated reactive oxygen species (ros), we cloned and characterized the cgap1 gene of c. gloeosporioides. cgap1 encoded a bzip transcription factor which had a bzip dna-binding domain and two cysteine-rich domains structurally and functionally related to saccharomyces cerevisi ...201728219524
comparative analysis of the mitochondrial genomes of colletotrichum gloeosporioides sensu lato: insights into the evolution of a fungal species complex interacting with diverse plants.the fungal species complex colletotrichum gloeosporioides sensu lato contains over 20 plant-interacting species. these species exhibit different life styles (e.g., endophytes, foliar and fruit pathogens) and show considerable variation in host and tissue adaptation strategies. accurate species delimitation in c. gloeosporioides s.l. is very challenging due to nascent lineage boundaries and phenotypic plasticity, which strongly impedes studies of the complex's host-interaction biology. in this st ...201728201983
application and bioactive properties of cati, a trypsin inhibitor from capsicum annuum seeds: membrane permeabilization, oxidative stress and intracellular target in phytopathogenic fungi cells.during the last few years, a growing number of antimicrobial peptides have been isolated from plants and particularly from seeds. recent results from our laboratory have shown the purification of a new trypsin inhibitor, named cati, from chilli pepper (capsicum annuum l.) seeds. this study aims to evaluate the antifungal activity and mechanism of action of cati on phytopathogenic fungi and detect the presence of protease inhibitors in other species of this genus.201728139827
legitimacy and implications of reducing colletotrichum kahawae to subspecies in plant pathology. 201628119726
synthesis, characterization and antifungal efficacy of c-coordinated o-carboxymethyl chitosan cu(ii) complexes.a novel type of o-carboxymethyl chitosan schiff bases (o-cspx) was synthesized via a condensation reaction. after the coordination reaction of cupric ions, cu(ii) complexes (o-cspx-cu) were achieved. the theoretical structure of o-cspx-cu calculated by gaussian 09 reveals that the copper ions underwent dsp(2) hybridization, coordinated by the carbon atom in the p-π conjugate group and the oxygen atoms in the acetate ion. then, the structures were confirmed by ft-ir, (1)h nmr, cp-mas (13)c nmr, e ...201728115106
varietal dynamics and yam agro-diversity demonstrate complex trajectories intersecting farmers' strategies, networks, and disease experience.loss of varietal diversity is a worldwide challenge to crop species at risk for genetic erosion, while the loss of biological resources may hinder future breeding objectives. loss of varieties has been mostly investigated in traditional agricultural systems where variety numbers are dramatically high, or for most economically important crop species for which comparison between pre-intensive and modern agriculture was possible. varietal dynamics, i.e., turnover, or gains and losses of varieties b ...201628066500
mutation of area affects growth, sporulation, nitrogen regulation, and pathogenicity in colletotrichum gloeosporioides.the gata transcription factor area is a global nitrogen regulator that restricts the utilization of complex and poor nitrogen sources in the presence of good nitrogen sources in microorganisms. in this study, we report the biological function of an area homolog (the cgarea gene) in the fruit postharvest pathogen colletotrichum gloeosporioides. targeted gene deletion mutants of area exhibited significant reductions in vegetative growth, increases in conidia production, and slight decreases in con ...201728027951
purification, biochemical characterization, and antimicrobial activity of a new lipid transfer protein from coffea canephora, an agronomical crop of great economic importance, is also among the most commonly traded commodities in worldwide markets. antimicrobial peptides, which play a role in plant defense, have been identified and isolated particularly from seeds. we isolated and immunolocalized cc-ltp2, a new lipid transfer protein (ltp) from coffea canephora seeds. we report its antimicrobial activity against various phytopathogenic fungi of economic importance, and against the bacterium xanthomonas euvesica ...201627813588
production of gentisyl alcohol from phoma herbarum endophytic in curcuma longa l. and its antagonistic activity towards leaf spot pathogen colletotrichum gloeosporioides.endophytes from medicinal plants represent a potential source of bioactive compounds. during the present investigation, fungal endophytes were isolated from turmeric (curcuma longa), an important medicinal plant. a total of 207 endophytic fungal isolates were obtained from the rhizome of c. longa l. they were grouped into seven genera based on morphological and molecular data. the fungal endophytes of c. longa were evaluated for antifungal activity against colletotrichum gloeosporioides, the cau ...201627288000
production of 7,8-dihydroxy unsaturated fatty acids from plant oils by whole recombinant cells expressing 7,8-linoleate diol synthase from glomerella cingulata.the reaction conditions for the production of 7s,8s-dihydroxy-9,12(z,z)-octadecadienoic acid from linoleic acid by recombinant escherichia coli expressing 7,8-linoleate diol synthase from glomerella cingulata were optimized using response surface methodology. the optimal reaction conditions were ph 7.0, 18.6 °c, 10.8% (v/v) dimethyl sulfoxide, 44.9 g/l cells, and 14.3 g/l linoleic acid, with agitation at 256 rpm. under these conditions, recombinant cells produced 7,8-dihydroxy unsaturated fatty ...201627779383
the characterization of 1-(4-bromophenyl)-5-phenyl-1h-1,2,3-triazole on acute toxicity, antimicrobial activities, photophysical property, and binding to two globular proteins.1-(4-bromophenyl)-5-phenyl-1h-1,2,3-triazole (bpt) was a newly synthesized compound. the acute toxicities of bpt to mice by intragastric administration have been determined and the result indicates that the intragastric administration of bpt did not produce any significant toxic effect on kunming strain mice. it is also evaluated for the antimicrobial activity of bpt against three kinds of plant mycoplasma, fusarium wilt (race 4), colletotrichum gloeosporioides penz. and xanthomonas oryzae by di ...201627693762
the colletotrichum gloeosporioides rhob regulates camp and stress response pathways and is required for pathogenesis.rho gtpases regulate morphology and multiple cellular functions such as asexual development, polarity establishment, and differentiation in fungi. to determine the roles of cgrhob, a rho gtpase protein, here we characterized cgrhob in the poplar anthracnose fungus colletotrichum gloeosporioides. first of all, we determined that conidial germination was inhibited and intracellular cyclic amp (camp) level was increased in the cgrhob deletion mutants. loss of cgrhob resulted in shorter germ tubes a ...201627670809
anti-respiratory syncytial virus compounds from two endophytic fungi isolated from nigerian medicinal plants.background: respiratory syncytial virus (rsv) is known to cause severe respiratory infections particularly in infants younger than 2 years of age. the only approved drug, ribavirin, is expensive and is not likely to improve therapeutic outcome, thereby necessitating the search for safer and more potent alternatives from natural sources such as endophytic fungi. the present study aimed to investigate the anti-rsv activity of compounds from endophytic fungi. methods: two endophytic fungi colletotr ...201627463031
antifungal activity of 1-methylcyclopropene (1-mcp) against anthracnose (colletotrichum gloeosporioides) in postharvest mango fruit and its possible mechanisms of action.anthracnose caused by colletotrichum gloeosporioides is one of the most important postharvest diseases in mango fruit, often causing huge economic losses. in this study, the effect of 1-methylcyclopropene (1-mcp) against anthracnose in postharvest mango fruit and the mechanisms involved were investigated. 1-mcp induced reactive oxygen species (ros) generation, damaged the mitochondria and destroyed the integrity of plasma membrane of spores of c. gloeosporioides, significantly suppressing spore ...201727728853
postharvest disease control of colletotrichum gloeosporioides and penicillium expansum on stored apples by gamma irradiation combined with study the control of postharvest decay caused by colletotrichum gloeosporioides and penicillium expansum, gamma irradiation alone or in combination with fumigation was evaluated to extend the shelf life of apples in south korea. an irradiation dose of 2.0 kgy resulted in the maximum inhibition of c. gloeosporioides and p. expansum spore germination. the gamma irradiation dose required to reduce the spore germination by 90% was 0.22 and 0.35 kgy for c. gloeosporioides and p. expansum, respecti ...201627721696
molecular characterization of a trisegmented mycovirus from the plant pathogenic fungus colletotrichum gloeosporioides.a novel double-stranded rna (dsrna) mycovirus, consisting of three dsrna genome segments and possibly belonging to the family chrysoviridae, was isolated from the filamentous phytopathogenic fungus colletotrichum gloeosporioides and designated as colletotrichum gloeosprioides chrysovirus 1 (cgcv1). the three dsrnas of the cgcv1 genome with lengths of 3397, 2869, and 2630 bp (dsrnas1-3) were found to contain a single open reading frame (orf) putatively encoding the rna-dependent rna polymerase (r ...201627690081
molecular and phenotypic characterization of colletotrichum species associated with anthracnose disease in peppers from sichuan province, china.the anthracnose caused by colletotrichum species is an important disease that primarily causes fruit rot in pepper. eighty-eight strains representing seven species of colletotrichum were obtained from rotten pepper fruits in sichuan province, china, and characterized according to morphology and the glyceraldehyde-3-phosphate dehydrogenase (gapdh) sequence. fifty-two strains were chosen for identification by phylogenetic analyses of multi-locus sequences, including the nuclear ribosomal internal ...201627609555
efficacy of ginger oil and extract combined with gum arabic on anthracnose and quality of papaya fruit during cold storage.effect of 2.0 % ginger oil (go) and 1.5 % ginger extract (ge) in combination with 10.0 % gum arabic (ga) was evaluated for the postharvest control of anthracnose and maintaining quality of eksotika ii papaya fruit during storage at 12 ± 1 °c and 80-85 % rh. antifungal compounds present in go and ge were analyzed using gas chromatography and go was found to contain α-pinene, 1, 8-cineole and borneol, while only borneol was present in ge due to different extraction methods applied. the highest ant ...201627570268
bzip transcription factor cgap1 is essential for oxidative stress tolerance and full virulence of the poplar anthracnose fungus colletotrichum gloeosporioides.yeast ap1 transcription factor is a regulator of oxidative stress response. here, we report the identification and characterization of cgap1, an ortholog of yap1 in poplar anthracnose fungus colletotrichum gloeosporioides. the expression of cgap1 was highly induced by reactive oxygen species. cgap1 deletion mutants displayed enhanced sensitivity to oxidative stress compared with the wild-type strain, and their poplar leaf virulence was obviously reduced. however, the mutants exhibited no obvious ...201627544415
chemical composition, herbicidal and antifungal activity of satureja cuneifolia essential oils from spain.the chemical composition of essential oils from satureja cuneifolia growing in east spain was analyzed by gc, gc/ms. forty-five compounds accounting for 99.1% of the total oil were identified. camphor (47.6%), followed by camphene (13.6%) were the main compounds. their herbicidal and antifungal activity was tested in vitro against three weeds (amaranthus hybridus, portulaca oleracea and conyza canadensis) and eleven common pathogenic or saprophytic fungi (phytophthora citrophthora, p. palmivora, ...201627534131
colletotrilactam a-d, novel lactams from colletotrichum gloeosporioides gt-7, a fungal endophyte of uncaria rhynchophylla.four novel lactams, colletotrilactam a-d (1-4), along with six known compounds (5-10) were isolated from the culture broth of colletotrichum gloeosporioides gt-7, a fungal endophyte of uncaria rhynchophylla. the structures of these compounds were elucidated by comprehensive nmr spectroscopy. isolates were tested for monoamine oxidase (mao) inhibitory activity and compound 9 showed potent mao inhibitory activity with ic50 value of 8.93±0.34μg/ml, when the ic50 value of iproniazid as a standard wa ...201627520493
decreased biosynthesis of jasmonic acid via lipoxygenase pathway compromised caffeine-induced resistance to colletotrichum gloeosporioides under elevated co2 in tea seedlings.caffeine, the major purine alkaloid in tea has long been known for its role in plant defense. however, its effect on colletotrichum gloeosporioides that causes brown blight disease in tea is largely unknown especially under elevated co2. here we show that elevated co2 reduced endogenous caffeine content in tea leaves, but sharply increased susceptibility of tea to c. gloeosporioides. the expression of c. gloeosporioides actin gene was gradually increased during the postinoculation period. in con ...201627392179
endophytic cultivable bacterial community obtained from the paullinia cupana seed in amazonas and bahia regions and its antagonistic effects against colletotrichum gloeosporioides.guarana (paullinia cupana var. sorbilis) is a plant from the amazonas region with socio-economic importance. however, guarana production has been increasingly affected by unfavorable conditions resulting from anthracnose, caused by the colletotrichum fungal genus, which primarily affects mainly the amazonas region. the aim of the present study was to isolate bacterial endophytes from the seeds of guarana plants obtained from amazonas region and the northeast state of bahia, a region where this d ...201627343372
scar marker specific to detect magnaporthe grisea infecting finger millets (eleusine coracana).to determine the molecular variability and develop specific sequence characterized amplified region (scar) marker for the detection of magnaporthe grisea causing blast disease in finger millet.201627328753
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