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[identification of puumala like viruses in china].to confirm if puumala like viruses exist in china.200312870020
adaptation of puumala hantavirus to cell culture is associated with point mutations in the coding region of the l segment and in the noncoding regions of the s segment.we previously developed a model for studies on hantavirus host adaptation and initiated genetic analysis of puumala virus variants passaged in colonized bank voles and in cultured vero e6 cells. with the data presented in this paper, the sequence comparison of the wild-type and vero e6-adapted variants of puumala virus, strain kazan, has been completed. the only amino acid substitution that distinguished the two virus variants was found in the l protein, ser versus phe at position 2053. another ...200312885898
local variation in helminth burdens of bank voles (clethrionomys glareolus) from ecologically similar sites: temporal stability and relationships with hormone concentrations and social behaviour.populations of bank voles (clethrionomys glareolus) in a fragmented forest habitat in north-east poland showed local differences in helminth infection intensity, morphometric measures and organ weights that were consistent with differences at the same locations two years previously. although overall intensities of infection were lower than previously, and there were some differences in the relative intensities of individual helminth species, site differences remained significant and were consist ...200312895277
hantavirus antibody occurrence in bank voles (clethrionomys glareolus) during a vole population cycle.puumala virus, genus hantavirus, is the etiologic agent of nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome. the bank vole (clethrionomys glareolus) is the natural reservoir species of this hantavirus. we initiated sampling of bank voles at sites of recently identified human nephropathia epidemica cases and paired control sites in the fall of 1995 in coastal areas of northern sweden. sites were trapped annually in spring and fall until 1999. prevalence of antibody to ...200312910756
behavioural development of conspecific odour preferences in bank voles, clethrionomys glareolus.biological odours of conspecifics are known to have strong influences on behavioural interaction in bank voles clethrionomys glareolus. this experiment tested two hypotheses. (1) olfactory cues from familiar and unfamiliar mature opposite-sex conspecifics differ in their attractiveness to males and females, and their behavioural reactions change with age. (2) a genetically based mechanism is involved in female recognition of kin.in a two-choice preference test, prepubertal males and females were ...200312914993
[the seasonal dynamics of fleas (siphonaptera) on bank voles (clethrionomys glareolus) in the north part of novgorod region].the twelve flea species were revealed on bank voles. only four of them (amalaraeus penicillige, ctenophthalmus uncinatus, megabothris turbidus, peromescopsylla bidentata) were abundant in some seasons of the year. four other species (ct. agyrtes, hystrichopsylla talpae, p. silvatica, rhadinopsylla integella) are the parasites of bank vole too but their numbers were always low in the study area. the four last species (amphypsylla rossica, m. walkeri, doratopsylla dasycnema, palaeopsylla soricis) ...201012916396
prenatal stress modifies behavior in offspring of bank voles (clethrionomys glareolus).the aim of the present study was to investigate the effect of prenatal stress on behavior of adult bank vole males and females. pregnant females were submitted to stress by short encounters of two females at the same stage of pregnancy, to crowded condition by transfer to a cage with four to five nonpregnant females, or stressed by immobilization. the stressors were applied on days 13, 14, and 15 of pregnancy. the results indicate that prenatal stress influenced adult offspring behavior tested i ...200312954408
[scent glands in the bank vole]. 195213021140
[detection of a parasite similar to toxoplasma in clethrionomys glareolus schr. brain]. 195513261195
the development of the hypophysial vascular system in the bank-vole clethrionomys glareolus; a comparative study. 195713478391
[parasitic cysts in the brain of the vole clethrionomys glareolus]. 195913808859
experimental infection of clethrionomys glareolus and apodemus flavicollis with tick-borne encephalitis virus. 196314062331
hormonal control of the pubic symphysis of the skomer bank vole (clethrionomys skomerensis). 196314086159
[existence of an adrenal x zone in the red field vole (clethrionomys glareolus)]. 195414365027
[intestinal microflora of sorex araneus araneus l. and clethrionomys glareolus glareolus schreb. in natural conditions. i. quantitative and qualitative characteristics of the intestinal microflora]. 201714460560
[intestinal microflora of sorex araneus araneus l. and clethrionomys glareolus glareolus schreb. in natural conditions. ii. general characteristics of separate strains]. 201714460561
[intestinal microflora of sorex araneus araneus l. and clethrionomys glareolus glareolus schreb. in nature conditions. iii. seasonal variations]. 201714460562
hantavirus infections in europe.hantaviruses are enveloped rna viruses each carried by a specific rodent species. three hantaviruses, puumala, dobrava, and saaremaa viruses, are known to cause haemorrhagic fever with renal syndrome. in europe. puumala causes a generally mild disease, nephropathia epidemica, which presents most commonly with fever, headache, gastrointestinal symptoms, impaired renal function, and blurred vision, whereas dobrava infections often also have haemorrhagic complications. there are few available data ...200314522264
the level of aberrant cells in various tissues of bank vole depending on doses and radionuclide balance in organism.a regression analysis shows the direct linear relation between 137cs accumulation in rodents and the level of aberrant cells. for 90sr this trend was negative. the dose relationship was the same with 137cs. the trends were negative 1 month later after feeding of animals with clean food. correspondingly the dose relationship was also negative. the levels of cells with apoptosis features were different in animals from the control and the radiocontaminated sites. the analysis has revealed the corre ...201014569617
tick-host-borrelia population interactions: long-term records in eastern europe.during 10 spring and summer seasons (1992-2001), the dynamics of the epizootic process in natural foci were studied in taiga forests of the pre-ural region, russia. in these foci, borrelia garinii, borrelia afzelii and tick-borne encephalitis (tbe) virus circulate transmitted by their main vector tick, ixodes persulcatus. main parameters of the epizootic process were calculated for each season. in three seasons (1993, 1996, and 1999) all parameters characterizing the abundance of unfed nymphs an ...200214570135
borrelia burgdorferi s.l. in ixodes ricinus (acari: ixodidae) ticks collected from vegetation and small rodents in recreational areas of the city of poznań.during 1998-1999, ixodes ricinus (l.) populations were investigated in three different biotopes (deciduous, mixed, coniferous forest) situated in popular recreational areas in poznań, poland. in total, 1,123 questing ticks (1,002 nymphs, 69 males, 52 females) were collected by flagging vegetation. additionally, in 1998 between may and september small rodents were trapped and inspected for feeding ticks. altogether, 213 rodents of three species: apodemus agrarius pall., a. flavicollis melchior, c ...200314596285
preliminary molecular characterization of cryptosporidium parvum isolates of wildlife rodents from poland.isolates of cryptosporidium were collected from 3 species of woodland and field rodents (clethrionomys glareolus, microtus arvalis, and apodemus flavicollis) and were characterized by polymerase chain reaction amplification and sequencing of fragments of the oocyst wall protein (cowp) gene and of the 18s ribosomal rna gene. sequence analysis of these markers revealed that the animals were infected with c. parvum, and that the genotype involved was almost identical to the mouse genotype previousl ...200314627156
genetic similarity of puumala viruses found in finland and western siberia and of the mitochondrial dna of their rodent hosts suggests a common evolutionary origin.a total of 678 small mammals representing eight species were trapped in western siberia in 1999-2000 and assayed for the presence of hantaviruses. eighteen animals, all clethrionomys species, were antigen positive by enzyme-linked immunosorbent assay (elisa). small and medium genome segments were recovered by rt-pcr from six samples from clethrionomys glareolus and three from clethrionomys rufocanus. sequence comparison and phylogenetic analysis revealed that these hantaviruses were puumala viru ...200314636686
photoperiodic effects on body mass, energy balance and hypothalamic gene expression in the bank vole.we examined the effect of increasing photoperiod, at a constant low temperature, on the body mass and energy budget of the bank vole clethrionomys glareolus. simultaneously, we determined the hypothalamic gene expression of neuropeptides and receptors known to be involved in short-term energy balance. despite an increase in body mass (approximately 10% of initial mass), we found no significant changes in any energetic parameters (food intake, energy assimilation rate, resting metabolic rate and ...200414638843
biological characterisation of cryptosporidium parvum isolates of wildlife rodents in poland.the study was undertaken to characterise the c. parvum isolates originating from naturally infected woodland and field rodents: clethrionomys glareolus (cg), apodemus flavicollis (af) and microtus arvalis (ma). we found that the measurements of oocyst dimensions and oocyst morphology did not allow distinction between the parasite isolates from the 3 rodent species. the mean dimensions were: for cg 4.67 x 4.21, for af 4.65 x 4.14 and for ma 4.66 x 4.16. these 3 groups of isolates have produced si ...200314677907
two-year study of examination of blood from wild rodents for the presence of antiborrelian antibodies.the aim of our work was to find the positivity rate of antibodrrelian antibodies (igg) in wild-living rodents in a locality situated in north moravia, czech republic. results of a survey for heart rinses (172) and sera (2) antibodies to borrelia burgdorferi sensu lato (s.l.) from 6 species of 174 wild rodents from the northern part of the czech republic are presented. samples were obtained in 2001-2002 at one locality (studenka, 49 degrees 44', 18 degrees 05'). host samples included yellow-necke ...200314677912
type 1 diabetes in swedish bank voles (clethrionomys glareolus): signs of disease in both colonized and wild cyclic populations at peak density.colonized bank voles (clethrionomys glareolus) originating from sweden developed type 1 diabetes. animals became polydipsic, glucosuric, and hyperglycemic and gradually developed a lethal ketoacidosis. pancreas in animals with end-stage disease showed total destruction of islet cells. interestingly, also a high proportion of wild bank voles in cyclic populations that were trapped at (or close to) the cyclic population density peak frequently showed high blood glucose levels and pathological gluc ...200314679053
human hantavirus infections, sweden.the prevalent human hantavirus disease in sweden is nephropathia epidemica, which is caused by puumala virus and shed by infected bank voles (clethrionomys glareolus). to evaluate temporal and spatial patterns of this disease, we studied 2,468 reported cases from a highly disease-endemic region in northern sweden. we found that, in particular, middle-aged men living in rural dwellings near coastal areas were overrepresented. the case-patients were most often infected in late autumn, when engaged ...200314718081
variation in predation risk and vole feeding behaviour: a field test of the risk allocation hypothesis.many prey animals experience temporal variation in the risk of predation and therefore face the problem of allocating their time between antipredator efforts and other activities like feeding and breeding. we investigated time allocation of prey animals that balanced predation risk and feeding opportunities. the predation risk allocation hypothesis predicts that animals should forage more in low- than in high-risk situations and that this difference should increase with an increasing attack rati ...200414730443
analysis of genetic relationships and antimicrobial susceptibility of escherichia coli isolated from clethrionomys glareolus.eleven strains of escherichia coli were isolated from 54 bank voles living in the lomza landscape park of the narew river valley, indicating that e. coli is not common in the alimentary tract of these mammals. on the basis of pulsed-field gel electrophoresis and computer-assisted analysis, the isolates were grouped into six genotypes at similarities of 39%. chromosome length of e. coli under study differed by as much as 900 kb, ranging 2.7-3.6 mb. all strains were susceptible to amikacin and cip ...200314747972
[structure and seasonal dynamics of an ectoparasite community on the common shrew (sorex araneus) in the il'men'-volkhov lowland].species composition, seasonal dynamics, and a load of ectoparasites per individual and population of the common shrew sorex araneus have been examined in coniferous and mixed forests of the ilmen'-volkhov lowland (a neighbourhood of oskuy village, chudovo district, novgorod province) during the period 1999-2003. trapping of mammals was carried monthly, with exception of few accidental gaps. the gero traps were used for catching micromammalian hosts. the lines of trap were checked 2 times a day, ...200314964844
microarray technology for identification and distinction of hantaviruses.dna microarrays combine high-precision technology with advanced molecular biology to achieve high-throughput screening of dna fragments. in this study, we investigated the potential of the cdna microarray technique to identify and discriminate pcr derived amplicons from genetically highly similar viruses. the wide range of sequence variation among hantaviruses makes them suitable as a model for this purpose. the hantaviruses, carried by rodents, cause several hundred thousand cases of severe hum ...200414981768
immunoglobulin g antibodies to borrelia burgdorferi in game animals and small mammals in eastern slovakia.in a survey of game animals and small mammals, the sera of 185 animals were examined for the presence of immunoglobulin g antibodies to the spirochete borrelia burgdorferi sensu lato, which causes lyme borreliosis. these animals comprised 59 fallow deer (dama dama), 56 mouflons (ovis musimon) and 70 small mammals of six different species. the sera of the fallow deer and the mouflons were examined by indirect haemagglutination assay. the sera of the small mammals were examined by modified enzyme- ...200315005560
[puumala and dobrava viruses in the northeastern and central regions of bosnia].bosnia and herzegovina has been known as a highly endemic region for hantavirus infections for more than 50 years. previous studies have shown that at least two different hantaviruses, the murine dobrava (dob) and avricoline puumala (puu) viruses, each carried by a different rodent species, have been circulating in the area. however, there is little information on rodent population density fluctuations in bosnia over the past years as well as on the ratio of puumala to dobrava infection in human ...200315011464
[what is new in the epidemiologic characteristics of hemorrhagic fever with renal syndrome in croatia?].the aim was to describe epidemiologic characteristics of hemorrhagic fever with renal syndrome in croatia.200315011468
photoperiod affects hepatic and renal cadmium accumulation, metallothionein induction, and cadmium toxicity in the wild bank vole (clethrionomys glareolus).the objective of this study was to examine the toxic effects of dietary cadmium (cd) on bank voles, being the f1 offspring of a wild-caught population. for 6 weeks, the rodents were provided with diets containing 0.05 (control), 40, 80, and 120 microg cd/g dry wt of diet under moderate (12 h) and long (16 h) photoperiods. histological examinations and analyses of metallothionein (mt), cd, cd bound and not bound to mt, iron and lipid peroxidation in the liver and kidneys were carried out. histopa ...200415087160
fluoride accumulation and toxicity in wild small mammals.populations of two species of small mammal, the field vole (microtus agrestis l.) and the bank vole (clethrionomys glareolus l.), inhabiting grasslands contaminated by industrial sources of fluoride were examined for fluoride concentrations in skeletal tissue and for morphological changes in the teeth. concentrations of fluoride in teeth and bones were higher for c. glareolus than for m. agrestis at the chemical works and smelter sites. severe dental lesions were recorded on the incisor and mola ...199415091672
bank voles as monitors of environmental contamination by heavy metals. a remote wilderness area in poland imperiled.concentrations of heavy metals (cd, pb, cu, zn, fe) were determined in the tissues of bank voles collected in polluted forest sites in southern poland and in białowieza national park. the highest cadmium concentrations were found in livers (0.9-12.8 microg g(-1)) and kidneys (3.2-29.6 microg g(-1)), whereas the lowest were in bones (0.2-0.9 microg g(-1)). the lead concentrations found in kidneys and bones ranged from 9.5 to 40.0 microg g(-1). the ranges of zinc, iron and copper concentrations in ...199015092204
the heavy metal content of the teeth of the bank vole (clethrionomys glareolus) as an exposure marker of environmental pollution in poland.this investigation is based on the premise that heavy metals are sequestered by the mineral phase of teeth, hydroxyapatite, during their formation. once formed these tissues are not subject to significant turnover and it is suggested, therefore, that they provide a permanent and cumulative record of heavy metal exposure during the development of the teeth. the teeth of the bank vole (clethrionomys glareolus) were analysed by inductively coupled plasma mass spectrometry to monitor the animals' ex ...200015092823
arsenic contamination in wood mice (apodemus sylvaticus) and bank voles (clethrionomys glareolus) on abandoned mine sites in southwest britain.arsenic can be highly toxic to mammals but there is relatively little information on its transfer to and uptake by free-living small mammals. the aim of this study was to determine whether intake and accumulation of arsenic by wild rodents living in arsenic-contaminated habitats reflected environmental levels of contamination and varied between species, sexes and age classes. arsenic concentrations were measured in soil, litter, wood mice (apodemus sylvaticus) and bank voles (clethrionomys glare ...200015092868
individual variation and repeatability of basal metabolism in the bank vole, clethrionomys glareolus.basal metabolic rate (bmr) is a fundamental energetic trait and has been measured in hundreds of birds and mammals. nevertheless, little is known about the consistency of the population-average bmr or its repeatability at the level of individual variation. here, we report that average mass-independent bmr did not differ between two generations of bank voles or between two trials separated by one month. individual differences in bmr were highly repeatable across the one month interval: the coeffi ...200415101695
zinc protection from fluoride-induced testicular injury in the bank vole (clethrionomys glareolus).previous work has shown that a high fluoride intake in rodents leads to histopathological changes in the germinal epithelium of testes that is associated with zinc deficiency. the purpose of this study was to determine whether supplemental dietary zn would protect against testicular toxicity induced by fluoride in a small rodent, the bank vole. the 4-month exposure period to fluoride (200 microg/ml of drinking water) induced histopathological changes (hemorrhage in interstitium, necrosis and apo ...200415104114
genetic basis of the trade-off between offspring number and quality in the bank vole.one of the main tenets of modern life-history theory is the negative relationship (trade-off) between the number and quality of offspring produced. theory predicts a negative genetic correlation between these traits since both are closely related to fitness of individuals. however, the genetic basis of the trade-off has only been tested to a limited extent in natural populations. we examined whether size and quality of offspring are negatively related to litter size in the bank vole clethrionomy ...200415119447
saaremaa hantavirus in denmark.most human hantavirus infections in denmark have been registered on the fyn island with puumala hantavirus hosted by clethrionomys glareolus as the causative agent. to search for other known european hantaviruses in denmark, we analyzed 101 apodemus spp. mice trapped on the lolland island. genome sequences of saaremaa hantavirus (saav) were recovered from one of the a. agrarius, thus suggesting saav presence in denmark. we also analyzed serum samples from a patient from lolland with a history of ...200415135745
the zoonotic reservoir of borrelia burgdorferisensu lato in the mazury lakes district of north-eastern poland.the roles of two species of rodents: the bank vole (clethrionomys glareolus) and the yellow-necked mouse (apodemus flavicollis) in maintaining the vector ixodes ricinus and borrelia burgdorferi s.l. were studied. more particularly, seasonal patterns of rodent abundance and infestation rate with i. ricinus ticks as well as the prevalence of b. burgdorferi s.l. and the two species b. garinii and b. afzelii were determined. the studies were carried out in woodlands at urwitałt near mikołajki on the ...200415147002
[long-term dynamics of the epizootic process in natural foci of ixodid tick borreliosis in mountain taiga forests of the middle ural].during eleven spring-summer seasons (1992-2002), the dynamics of the epizootic process in natural foci of ixodid tick-borne borrelioses (itbb) were studied in taiga forests of the middle ural (perm province, russia). in these foci, borrelia garinii and b. afzelii circulate, and their main vector is the ixodes persulcatus tick. main parameters of the epizootic process were calculated for each season. in four seasons (1993, 1996, 1999 and 2002), the parameters characterizing the abundance of unfed ...200415174388
rapid field test for detection of hantavirus antibodies in rodents.puumala virus (puuv) is the causative agent of nephropathia epidemica, a mild form of haemorrhagic fever with renal syndrome. puuv is transmitted to humans via aerosolized excreta of the infected bank vole (clethrionomys glareolus). current methods for screening of the puuv prevalence among bank vole populations are laborious, combining sampling in the field and subsequent analyses in the laboratory. in order to facilitate animal testing, a new serological immunochromatographic rapid test was de ...200415188724
flea-borne bartonella grahamii and bartonella taylorii in bank voles.bartonella species are increasingly associated with a range of human and animal diseases. despite this, we have a poor understanding of the ecology and epidemiology of many species, especially those circulating in wild populations. previous studies have demonstrated that a diverse range of bartonella species are abundant in wild rodent populations; little is known regarding their modes of transmission, although both direct and indirect routes have been suggested. in this study, with bank voles ( ...200415200860
further investigation of the heavy metal content of the teeth of the bank vole as an exposure indicator of environmental pollution in poland.the content of heavy metals in mineralised dental tissues is an indicator of the exposure of their mineral phase to heavy metals during the time of tooth development and dental tissue formation. therefore, teeth have been used as bio-indicators which accurately reflect the environmental or dietary exposure of animals and humans to heavy metals. this study follows from the earlier work in which it was demonstrated that the teeth from bank voles inhabiting various environmentally polluted and non- ...200415210277
detection of anaplasma phagocytophilum in animals by real-time polymerase chain reaction.the aim of this study was to detect anaplasma phagocytophilum in wild and domesticated animals and to identify the phylogenetic relationships of different strains of this bacterium. we adapted six published conventional methods targeting 16s fragments for real-time polymerase chain reaction. initial screening of samples from 419 animals found 37 anaplasma positives, later confirmed with several different primers and a taqman probe. we also performed dna quantification and melting curve analysis. ...200415233638
small mammals (insectivora, rodentia) as a potential source of chlamydial infection in east slovakia.the presence of antibodies against chlamydophila (chlamydia) psittaci in small mammals (insectivora, rodentia) in the region of east slovakia are presented. the hosts were caught in several areas of slovakia in habitats with different levels of anthropogenic disturbance. research was carried out during 2000-2002. the authors examined 1,947 sera coming from 4 insectivore and 10 rodent species. each serum was examined by micromethod of complement binding reactions using antigen chlamydophila (chla ...200415236511
histopathological changes in the liver, kidneys, and testes of bank voles environmentally exposed to heavy metal emissions from the steelworks and zinc smelter in poland.bank voles were trapped in the neighborhood of the sendzimir steelworks in krakow and the zgh boleslaw zinc smelter in bukowno. the borecka forest in the north of poland served as a control area. lead, cadmium, zinc, and iron concentrations were analyzed in the liver, kidneys, testes, and femur bones of the bank voles. typically, high levels of lead and cadmium were found in the bones and kidneys, respectively. in the femurs of the rodents from bukowno, 109.26 microg g(-1) dry weight of lead was ...200415261786
characterization of tula virus from common voles (microtus arvalis) in poland: evidence for geographic-specific phylogenetic clustering.tula virus (tulv), a recently identified arvicolid rodent-borne hantavirus, is harbored by the european common vole (microtus arvalis) in central russia and the czech and slovak republics. we report the isolation and characterization of this hantavirus from m. arvalis captured in poland, a country where human disease caused by hantaviruses has not been recognized. of 34 arvicolid rodents (24 clethrionomys glareolus, 9 m. arvalis, 1 pitymys sp.) captured in lodz and tuszyn, poland, during june to ...200415284484
hormonal regulation of oestrogen formation by leydig cells in vitro: immunocytochemical approach.the ability of the testis to convert androgens into oestrogens is related to the presence of a microsomal enzyme, aromatase, in testicular cells. the aim of this study was to show whether the supplementation of culture media with lh or an aromatase inhibitor could affect the process of aromatisation in leydig cells of the bank vole in vitro. this was investigated by means of immunocytochemistry and radioimmunological assays. in control cultures of leydig cells, both steroid hormones secretion as ...200315303372
dose- and photoperiod-dependent effects of 17beta-estradiol and the anti-estrogen ici 182,780 on testicular structure, acceleration of spermatogenesis, and aromatase immunoexpression in immature bank voles.it has been known that administration of estrogens or deficiency of estrogens can affect development and/or maintenance of male gonadal functions. these hormones are able to control germ cell development, and especially spermatid production and epididymis sperm maturation. the aim of the present study was to show the effects of 17beta-estradiol and a pure anti-estrogen, ici 182,780, on the bank vole testis. immature bank voles reared under either short or long light cycles were injected intraper ...200415350809
marked species and age-dependent differences in cell proliferation and neurogenesis in the hippocampus of wild-living rodents.variations in the extent of adult neurogenesis and natural and experimental factors controlling it have been described in laboratory animals. the wide range of variation seen even within a species, the mouse, raises the question as to which rates of neurogenesis can be expected in natural populations. answering this question is important to evaluate the functional significance of adult neurogenesis under natural conditions and to define the factors controlling it. to address this issue, we inves ...200415390172
frenkelia parasites in a small mammal community. dynamics of infection and effect on the host.a community of small mammals, clethrionomys glareolus, arvicola terrestris, microtus arvalis, m. agrestis, m. subterraneus, apodemus spp. and sorex spp., was studied as hosts of frenkelia glareoli and f. microti in fronche-comté (france). they were monitored in spring, summer and autumn on an area of about 1,350 ha comprising open field, hedgerow network and forest. among 1,714 small mammals examined between july 1992 and october 1993, 47% (178/376) of c. glareolus, 9.9% (14/139) of a. terrestri ...200415490755
identification of bartonella species in rodents, shrews and cats in denmark: detection of two b. henselae variants, one in cats and the other in the long-tailed field mouse.small mammals and stray cats were trapped in two areas of north zealand, denmark, and their blood cultured for hemotrophic bacteria. bacterial isolates were recovered in pure culture and subjected to 16s rdna gene sequencing. bartonella species were isolated from five mammalian species: b. grahamii from microtus agrestis (field vole) and apodemus flavicollis (yellow-necked field mouse); b. taylorii from m. agrestis, a. flavicollis and a. sylvaticus (long-tailed field mouse); b. tribocorum from a ...200415511270
granule cell number, cell death and cell proliferation in the dentate gyrus of wild-living rodents.adult neurogenesis in the dentate gyrus occurs at species-specific levels. wood mice (apodemus flavicollis) show higher proliferation rates than laboratory mice and voles (clethrionomys glareolus, microtus subterraneus). we compare rates of cell death and proliferation and investigate if cell proliferation leads to the long-term recruitment of granule cells. granule and pyknotic cell numbers were estimated in wild-living rodents in different age classes and compared with laboratory mice of mixed ...200415610166
anti-borrelia antibodies in rodents: important hosts in ecology of lyme disease.data presented in this study focuses on the presence of anti-borrelia antibodies in small mammals from eastern slovakia during 2000-2003. the total seropositivity observed was 18.78% in rodents. amongst all species, the total seroprevalence in apodemus flavicolis was the highest (20.87%), followed by apodemus agrarius (19.58%) and clethrionomys glareolus (11.11%). however, the prevalence in apodemus flavicolis during the year 2000-2001 was higher (26.72%), which reduced to 10.60% in 2002-2003. t ...200415627326
a comparison of wild-caught wood mice and bank voles in the intellicage: assessing exploration, daily activity patterns and place learning paradigms.our previous work has revealed very high baseline neurogenesis in the dentate gyrus of wood mice as compared particularly to bank voles; a difference which may be related to learning capacity. this study explored whether the newly-developed intellicage system could be used to compare these species in simple spatial learning paradigms. the intellicage is essentially a group-housing cage that also allows continuous automatic recording of each individual's behaviour. seven wild-caught bank voles (c ...200515639172
[structure of parasitic arthropod communities in forest small mammals].species composition and structure of ectoparasite arthropod communities were examined all year round six years in the bank vole clethrionomys glareolus, ural wood mouse apodemus uralensis and the common shrew sorex araneus in forests of the ilmen'-volkhov depression. in total, 4500 host samples have been examined and all ectoparasites have been collected. the species composition of ectoparasite community in small mammal species are as follows: the bank vole--29 insect, tick and mite species, the ...200415656091
testicular toxicity induced by dietary cadmium is associated with decreased testicular zinc and increased hepatic and renal metallothionein and zinc in the bank vole (clethrionomys glareolus).mechanism of testicular toxicity induced by dietary cadmium (cd) has been less investigated than that following acute cd injection. in the present study we characterized testicular injury in a small rodent, the bank vole, exposed subchronically to dietary cd in a quantity of 0.9 micromol/g, and determined the importance of some factors (cd accumulation, metallothionein (mt), oxidative stress, and zinc (zn)) in the injury. dietary cd induced moderate histopathological changes (hemorrhage in inter ...200415689104
predator-induced synchrony in population oscillations of coexisting small mammal species.comprehensive analyses of long-term (1977-2003) small-mammal abundance data from western finland showed that populations of microtus voles (field voles m. agrestis and sibling voles m. rossiaemeridionalis) voles, bank (clethrionomys glareolus) and common shrews (sorex araneus) fluctuated synchronously in 3 year population cycles. time-series analyses indicated that interspecific synchrony is influenced strongly by density-dependent processes. synchrony among microtus and bank voles appeared addi ...200515695211
characterization of borrelia burgdorferi sensu lato isolated in moscow province--a sympatric region for ixodes ricinus and ixodes persulcatus.the relationships between borrelia species and the vector ticks, ixodes persulcatus and ixodes ricinus were examined in a molecular epidemiological study. we conducted a survey in the moscow region which is a sympatric region for both species of tick. we examined 630 unfed i. ricinus and i. persulcatus, ticks collected from four different regions around moscow within an area of 250 km2. eighty-four ticks were culture positive (13.3%) and the prevalence rate varied in each region from 5.7% to 42. ...200515715174
sheep grazing and rodent populations: evidence of negative interactions from a landscape scale experiment.inter-specific competition, facilitation and predation influence herbivore assemblages, but no study has experimentally explored the interactions between large ungulates and small rodents. in a fully replicated, landscape scale experiment, we manipulated densities of domestic sheep in mountain pastures in norway. we then determined population growth and densities of rodents by live trapping in each of the areas with different sheep densities. we found that the (summer) population growth rate and ...200515726430
medium-term temporal stability of the helminth component community structure in bank voles (clethrionomys glareolus) from the mazury lake district region of poland.the structure of helminth communities in wild rodents is subject to seasonal variation, and is dependent on host age within years. although between-year variation has been monitored, seldom has it been assessed rigorously by appropriate multifactorial analysis with potentially confounding factors taken into account. in this study we tested the null hypothesis that despite seasonal, host age and sex effects, helminth communities should show relative stability between years. over a period of 3 yea ...200515727071
identification of a new borrelia species among small mammals in areas of northern spain where lyme disease is endemic.the role of small mammals as reservoir hosts for borrelia burgdorferi was investigated in several areas where lyme disease is endemic in northern spain. a low rate of infestation by ixodes ricinus nymphs was found in the small mammal populations studied that correlated with the near-absence of b. burgdorferi sensu lato in 184 animals tested and with the lack of transmission of b. burgdorferi sensu lato to i. ricinus larvae that fed on them. in contrast, questing ticks collected at the same time ...200515746336
effect of photoperiod on cultured granulosa cells of the bank vole, clethrionomys glareolus.gonadal function of the bank vole females depends on the photoperiod. this experiment was to show whether photoperiod applied on the whole animal in vivo would affect the function of ovarian cells in vitro. granulosa cells from large ovarian follicles of bank vole reared in long or short photoperiod were cultured as monolayers in control or luteinizing hormone supplemented media. formation of cell colonies, activity of delta5, 3beta-hydroxy steroid dehydrogenase and progesterone secretion were i ...200515748858
beyond the mediterranean peninsulas: evidence of central european glacial refugia for a temperate forest mammal species, the bank vole (clethrionomys glareolus).this study details the phylogeographic pattern of the bank vole, clethrionomys glareolus, a european rodent species strongly associated with forest habitat. we used sequences of 1011 base pairs of the mitochondrial dna cytochrome b gene from 207 bank voles collected in 62 localities spread throughout its distribution area. our results reveal the presence of three mediterranean (spanish, italian and balkan) and three continental (western, eastern and 'ural') phylogroups. the endemic mediterranean ...200515836645
genetic correlations between basal and maximum metabolic rates in a wild rodent: consequences for evolution of endothermy.according to the aerobic capacity model, endothermy in birds and mammals evolved as a correlated response to selection for an ability of sustained locomotor activity, rather than in a response to direct selection for thermoregulatory capabilities. a key assumption of the model is that aerobic capacity is functionally linked to basal metabolic rate (bmr). the assumption has been tested in several studies at the level of phenotypic variation among individuals or species, but none has provided a cl ...200515856708
androgen receptor in the ovary of postnatal bank vole females.in this experiment, we investigated the influence of the photoperiod upon androgen receptor (ar) distribution and steroid concentrations in ovaries of 21-day-old bank vole females. sections (6 mum) were assayed immunohistochemically. ars were localized in the nuclei of granulosa cells. the strongest immunoreaction was observed in primary and early antral follicles, and declined during follicular development. p(4), a, and e(2) contents were measured by rias in ovarian homogenates from animals kep ...200515891050
cluster of capillaria hepatica infections in non-commensal rodents from the canton of geneva, switzerland.we trapped 664 rodents belonging to five non-commensal species in the canton of geneva, switzerland, and found a significant cluster of capillaria hepatica infections in three species in rural and urbanized areas of the northern part of the canton. c. hepatica infections were discovered in the yellow-necked mouse apodemus flavicollis (n=99) with an overall prevalence (op) of 7.0% and a clustered prevalence (cp) of 20%, in the bank vole clethrionomys glareolus (n=58, op 5.2%; cp 19%), and in the ...200515924224
hepatic and renal cadmium accumulation is associated with mass-specific daily metabolic rate in the bank vole (clethrionomys glareolus).previous study has shown that photoperiod and age affect tissue accumulation of cadmium (cd) in a small rodent, the bank vole. since the body mass is also influenced by these factors, the present study was designed to determine whether mass-specific daily metabolic rate might be responsible for differential accumulation of cd in the liver and kidneys of the short- and long-photoperiod bank voles as well as of the young and old animals. one- and five-month old male bank voles were held under shor ...200515950545
disruption of a host-parasite system following the introduction of an exotic host species.the potential of biological invasions to threaten native ecosystems is well recognized. here we describe how an introduced species impacts on native host-parasite dynamics by acting as an alternative host. by sampling sites across an invasion front in ireland, we quantified the influence of the introduced bank vole (clethrionomys glareolus) on the epidemiology of infections caused by flea-transmitted haemoparasites of the genus bartonella in native wood mice (apodemus sylvaticus). bartonella inf ...200515977903
melatonin increases tissue accumulation and toxicity of cadmium in the bank vole (clethrionomys glareolus).recent study has shown that a short photoperiod increases the accumulation and toxicity of cadmium (cd) in the bank vole as compared to a long photoperiod. since many of the effects of photoperiod on physiological processes in small mammals are transduced by the pineal gland and its hormone melatonin, in this study the effect of subchronic melatonin injection (7 micromol/kg/day for 6 weeks) on the hepatic, renal and intestinal cd accumulation in the bank voles raised under a long photoperiod and ...200515984572
identification of the pathological prion protein allotypes in scrapie-infected heterozygous bank voles (clethrionomys glareolus) by high-performance liquid chromatography-mass spectrometry.cerebral formation of the pathological isoform of the prion protein (prp) is a crucial molecular event in prion diseases. the bank vole (clethrionomys glareolus) is a rodent species highly susceptible to natural scrapie. the prp gene of bank vole is polymorphic (met/ile) at codon 109. here we show that homozygous 109met/met voles have incubation times shorter than heterozygous 109met/ile voles after experimental challenge with three different scrapie isolates. an hplc-ms/ms method was optimized ...200516013608
aromatase in testis: expression and role in male reproduction.the mammalian testis serves two main functions: production of spermatozoa and synthesis of steroids, among them estrogens are the end products obtained from the irreversible transformation of androgens by aromatase (p450arom). in the rat the pattern of p450arom expression differs among the testicular somatic cell types according to age; in addition, we have shown that gonocytes, spermatogonia, spermatocytes (preleptotene, pachytene), spermatids and spermatozoa, represent an important source of e ...200516019206
paradoxical rate-dependent effect of fluoxetine on captivity-induced stereotypies in bank voles.treatment of stereotypies in human and animal clinics is still empirical due to our incomplete understanding of underlying mechanisms. as a consequence, experimental studies in controlled laboratory conditions are necessary. bank voles (clethrionomys glareolus), housed in barren laboratory cages, often develop stereotyped up-and-down jumping even before the age of 1 month. the aim of this study was to investigate how captivity-induced stereotypies respond to fluoxetine (prozac), a selective sero ...200516023277
metals distribution and interactions in tissues of shrews (sorex spp.) from copper- and zinc-contaminated areas in poland.to assess the risk from heavy metal accumulation to insectivorous species exposed to different pollutants, shrews [sorex araneus (linnaeus 1758) and sorex minutus (linnaeus 1766)] were collected in the olkuski ore region (oor; a zn and cd smelter area), legnicko-głogowski copper mine region (lgcr; a copper ore-mining area), and białowieza forest (bf; a control area). a few sites were chosen in each region and a total of 57 animals were collected from them. the liver and kidneys were dissected fr ...200516091604
puumala virus in croatia in the 2002 hfrs outbreak.hfrs is an endemic disease throughout croatia. the incidence of hfrs varies in a cyclic fashion, with peaks occurring every couple of years, coinciding with peaks in vole populations. puuv was shown to be dominant pathogen during the last hfrs outbreak in croatia in 2002. we focused our research on two newly discovered localities (okucani and nova gradiska) with a high number of reported hfrs cases and a significant increase in rodent population. puuv infection was verified in 84.2% of patients ...200516121366
aromatase and oestrogens in human male germ cells.the mammalian testis serves two main functions: production of spermatozoa and synthesis of steroids, among them oestrogens are the end products obtained from the irreversible transformation of androgens by aromatase (p450arom). up today p450arom has been demonstrated in male germ cells of all mammals so far studied (mice, rat, bank vole, bear, monkey). in man leydig cells and immature germ cells as well as ejaculated spermatozoa express a biologically active aromatase. moreover germ cells and sp ...200516128984
the distribution of arsenic in the body tissues of wood mice and bank voles.arsenic is accumulated by free-living small mammals, but there is little information on the resultant concentrations in different tissues other than liver and kidney. such information is important because the severity of toxicological effects may be related to the amount of arsenic accumulated in specific organs, and the availability of arsenic to predators is, in part, dependent on which tissues accumulate arsenic. the objective of this study was to quantify the arsenic concentrations and the p ...200516170450
long-term development of the radionuclide exposure of murine rodent populations in belarus after the chernobyl accident.as a determinant of the associated health risks, the behavior of radionuclides in natural ecosystems needs to be better understood. therefore, the activity concentration of various long-lived radionuclides released due to the chernobyl accident, and the corresponding contributions to the whole-body dose rate, was studied as a function of time in mammalian indicator species inhabiting the natural forest ecosystems of belarus, the bank vole (clethrionomys glareolus) and the yellow-necked mouse (ap ...200516215755
a mathematical model for indirectly transmitted diseases.we consider a mathematical model for the indirect transmission via a contaminated environment of a microparasite between two spatially distributed host populations having non-coincident spatial domains. the parasite is benign in a first population and lethal in the second one. global existence results are given for the resulting reaction-diffusion system coupled with an ordinary differential equation. then, invasion and persistence of the parasite are studied. a simplified model for the transmis ...200716216284
piximus dxa with different software needs individual calibration to accurately predict fat mass.to validate ge piximus2 dxa fat mass (fm) estimates by chemical analysis, to compare previously published correction equations with an equation from our machine, and to determine intermachine variation.200516222058
aromatization and antioxidant capacity in the testis of seasonally breeding bank voles: effects of lh, prl and igf-i.aromatization and antioxidant strategies in the male gonads are important processes, which are involved in control of normal fertility. the objective of this study was to show whether luteinizing hormone (lh), prolactin (prl), and insulin-like growth factor-i (igf-i) as well as the length of photoperiod are able to exert an effect on aromatase expression, steroid hormone levels, and antioxidant concentrations in testes of bank voles, seasonally breeding rodents. mature bank voles that were kept ...200616226304
[patterns of host-parasite interactions between the nematode heligmosomum mixtum (schulz, 1952) and the bank vole (clethrionomys glareolus schreber, 1780)].some aspects of the host-parasite interaction of the nematode heligmosomum mixtum and the bank vole have been studied. the dependence of infestation on the host sex, age and weight, the seasonal and annual abundance dynamics are investigated. it has been found that the bank vole is the main host of h. mixtum; the distribution of h. mixtum abundances in the host population is described by a negative binomial distribution model. the greatest infestation is characteristic for mature bank voles; mal ...200516316059
hosts and pathogen detection for immature stages of ixodes ricinus (acari: ixodidae) in north-central spain.to determine hosts of the immature stages of a southern population of ixodes ricinus, we trapped rodents and birds in an area of north-central spain in may-june and august-september of 1998 and 1999. the most frequently trapped rodents were apodemus sylvaticus (230 specimens) and clethrionomys glareolus (99), with a larval infestation prevalence of 49% and 81%, respectively (in spring) and 21% and 41% (in summer). c. glareolus was always more heavily parasitized by larvae (mean numbers 19.8 in s ...200516323055
explaining bank vole cycles in southern norway 1980-2004 from bilberry reports 1932-1977 and climate.correlations between mast fruiting of bilberry vaccinium myrtillus and peak levels of clethrionomys-voles have been reported from both norway and finland, but there has been a discussion whether this is a bottom-up or a top-down relationship. in a multiple regression model, 65% of the variation in a bilberry production index calculated from game reports from southern norway 1932-1977 could be explained by the berry index of the two preceding years and climate factors acting during key stages of ...200616344969
borrelia burgdorferi sensu stricto in yellow-necked mice and feeding ixodes ricinus ticks in a forest habitat of west central poland.wild rodents and the subadult ixodes ricinus (l.) ticks infesting them were examined for the presence of borrelia burgdorferi johnson, schmid, hyde, steigerwalt & brenner s.l. in a sylvatic habitat in west central poland during may-september 2002. in total, 818 feeding ticks were recovered from 73 infested yellow-necked mice, apodemus flavicollis melchior; in addition, bank voles, clethrionomys glareolus schreber, were rarely captured and proved to be weakly parasitized. only 2.7% of a. flavicol ...200516363171
hantavirus and arenavirus antibody prevalence in rodents and humans in trentino, northern italy.the spatial and temporal distribution of hantavirus and arenavirus antibody-positive wild rodents in trentino, italy, was studied using immunofluorescence assays (ifa) in two long-term sites trapped in 2000-2003, and six other sites trapped in 2002. the overall hantavirus seroprevalence in the bank voles, clethrionomys glareolus (n=229) screened for puumala virus (puuv) antibodies was 0.4%, and that for apodemus flavicollis mice (n=1416) screened for dobrava virus (dobv) antibodies was 0.2%. ant ...200616371172
habitat factors associated with bank voles (clethrionomys glareolus) and concomitant hantavirus in northern sweden.puumala virus (puuv), genus hantavirus, causes nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome in humans. in this study, bank voles, the natural reservoir of puuv, were captured at locations of previous human puuv exposure and paired controls within a region of high incidence in northern sweden. the aim of the study was to evaluate the influence of environmental factors on the abundance of bank voles and the occurrence of puuv. the total number of voles and the numbe ...200516417427
the distribution and structure of the lingual papillae on the tongue of the bank vole clethrinomys glareolus.the distribution and three-dimensional structure of the lingual papillae were studied by means of scanning electron microscopy. the elongated tongue in the bank vole is about 12 mm in length and about 3 mm in width. the characteristic features of the tongue are the median sulcus on the apex of the tongue, considerable narrowing in the body of the tongue and a well developed intermolar prominence. on the surface of the apex and body of the tongue three morphological types of the filiform papillae ...200516425161
conversion efficiency of bank vole prion protein in vitro is determined by residues 155 and 170, but does not correlate with the high susceptibility of bank voles to sheep scrapie in vivo.the misfolded infectious isoform of the prion protein (prp(sc)) is thought to replicate in an autocatalytic manner by converting the cellular form (prp(c)) into its pathogenic folding variant. the similarity in the amino acid sequence of prp(c) and prp(sc) influences the conversion efficiency and is considered as the major determinant for the species barrier. we performed in vitro conversion reactions on wild-type and mutated prp(c) to determine the role of the primary sequence for the high susc ...200616455657
duplication, balancing selection and trans-species evolution explain the high levels of polymorphism of the dqa mhc class ii gene in voles (arvicolinae).major histocompatibility complex (mhc) genes play important role in host-parasite interactions and parasites are crucial factors influencing the population dynamics of hosts. we described the structure and diversity of exon 2 of the mhc class ii dqa gene in three species of voles (arvicolinae) exhibiting regular multi-annual fluctuations of population density and analysed the processes leading to the observed mhc polymorphism. by using cloning-sequencing methodology and capillary electrophoresis ...200616467985
efficient transmission and characterization of creutzfeldt-jakob disease strains in bank voles.transmission of prions between species is limited by the "species barrier," which hampers a full characterization of human prion strains in the mouse model. we report that the efficiency of primary transmission of prions from creutzfeldt-jakob disease patients to a wild rodent species, the bank vole (clethrionomys glareolus), is comparable to that reported in transgenic mice carrying human prion protein, in spite of a low prion protein-sequence homology between man and vole. voles infected with ...200616518470
variation in mitochondrial dna control region haplotypes in populations of the bank vole, clethrionomys glareolus, living in the chernobyl environment, ukraine.bank vole, clethrionomys glareolus, specimens have been annually sampled from the radioactive chernobyl, ukraine, environment and nonradioactive reference sites since 1997. exposed voles continually exhibit increased mitochondrial dna haplotype (h) and nucleotide diversity (nd), observed in the hypervariable control region (1997-1999). increased maternal mutation rates, source-sink relationships, or both are proposed as hypotheses for these differences. samples from additional years (2000 and 20 ...200616519312
risk assessment of metals and organic pollutants for herbivorous and carnivorous small mammal food chains in a polluted floodplain (biesbosch, the netherlands).a risk assessment was made for a carnivorous and a herbivorous food chain in a heavily polluted natural estuary (biesbosch), by determining the most critical pollutants and the food chain most at risk. exposure of food chains to metals, polycyclic aromatic hydrocarbons (pahs), and polychlorinated biphenyls (pcbs) was assessed by analyzing dietary concentrations, internal concentrations, and biomarkers of exposure. common shrew (sorex araneus) and bank vole (clethrionomys glareolus) were selected ...200616530312
bovine tuberculosis (mycobacterium bovis) in british farmland wildlife: the importance to agriculture.bovine tuberculosis (btb) is an important disease of cattle and an emerging infectious disease of humans. cow- and badger-based control strategies have failed to eradicate btb from the british cattle herd, and the incidence is rising by about 18%per year. the annual cost to taxpayers in britain is currently 74 million uk pounds. research has focused on the badger as a potential btb reservoir, with little attention being paid to other mammals common on farmland. we have conducted a systematic sur ...200616543179
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