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two kinds of monoclonal antibodies to tick-borne encephalitis virus.two types of monoclonal antibodies (ma) of the ken and nek series prepared to tick-borne encephalitis (tbe) virus differed in the spectrum of their reactivity in serological tests and in their ability to react with individual representatives of the tbe virus complex. the ken series ma were induced to the 4072 strain isolated from the blood of a patient in the u.s.s.r. the nek series ma were prepared to the skalica strain isolated from a bank vole in czechoslovakia. both groups of ma belonged to ...19862874724
ecological and epidemiological data on hantavirus in bank vole populations in belgium.epidemiological and ecological data of a hantavirus infected bank vole population were collected using live-trapping methods. a close association between the distribution of hv-infected bank voles and wet habitat types was found. several seroconversions were observed during the life of individual bank voles. all seroconversions occurred within the wet habitat types and only after a certain age was reached. survival of hv-infected bank voles did not differ from not-infected animals. in the labora ...19862877647
epidemiology of nephropathia epidemica in sweden.a comprehensive study of nephropathia epidemica (ne), caused by puumala virus, was conducted in sweden. human sera from residents of various regions of sweden were examined for antibody to puumala virus, and the incidence of ne was determined. small mammals were captured at locations throughout sweden and were examined for antibody to puumala virus and for antigen. the human serosurvey found the highest prevalence rates and the highest incidence rates in northern sweden. the bank vole was found ...19872879874
occurrence and distribution of hantavirus in wild living mammals in belgium.small mammals were screened for the presence of antibodies to hantaan virus (htn) and hantavirus (hv) antigen in belgium. antibody and antigen-positive animals were found in different parts of the country. one insectivore and five rodent species were found positive. the highest prevalence of infection was found in the bank vole (clethrionomys glareolus). a relation between infected animals and wet habitats was observed. it was obvious that in bank vole the likelihood of infection increased with ...19872883857
partial characterization of a hantavirus isolated from a clethrionomys glareolus captured in belgium.a hantavirus was isolated in vero-e6 cells from lungs of a free living bank vole (clethrionomys glareolus) captured in turnhout, province of antwerp--northern part of belgium. with help of monoclonal antibodies the belgian hantavirus isolate could be clearly differentiated from hantaan virus strain 76-118, prospect hill virus strain ph1 and sr11, a hantavirus isolated from laboratory wistar rat in japan, but not from the nephropathia epidemica virus strain hällnäs.19872886029
[detection of foci of hemorrhagic fever with renal syndrome in the estonian ssr].examination by the enzyme-immunoassay of organs of 10 small mammal species trapped in different landscape zones of the estonian ssr revealed the presence of hfrs virus antigen in organs of bank voles and field mice. radioimmunoassay studies of serum specimens from donors demonstrated the presence of antibody to hfrs virus in 2.54% of those examined.19872891222
hemorrhagic fever with renal syndrome in yugoslavia: detection of hantaviral antigen and antibody in wild rodents and serological diagnosis of human disease.lung tissues from 547 rodents and 26 insectivores captured between 1981 and 1984 in central bosnia (fojnica) and central serbia (cacak), 2 regions known to be endemic for hemorrhagic fever with renal syndrome (hfrs), were examined for hantaviral antigen by the indirect immunofluorescent antibody technique. antigen was detected in 17/231 apodemus flavicollis, 3/187 a. sylvaticus, 1/46 a. agrarius, 4/32 mus musculus, and 3/28 clethrionomys glareolus. in addition, antibodies against hantaan and puu ...19882900550
hemorrhagic fever with renal syndrome (nephropathia epidemica) in norway: seroepidemiology 1981-1985.an indirect fluorescent antibody technique (ifat) was applied to serologically confirm the clinical diagnosis in 507 nephropathia epidemica (ne) suspected patients. hantaan virus (hv), the agent of korean hemorrhagic fever, which is serologically related to the ne agent, was used as antigen. both igg and igm reactions were detected. high levels of igg antibodies to hv were common, even in the acute phase of illness. over a 5-year period, a total of 35% of the ne suspected patients revealed antib ...19882900551
seroepidemiology of haemorrhagic fever with renal syndrome in bulgaria.during the period of 1954-1986, 399 cases of haemorrhagic fever with renal syndrome (hfrs) were registered in bulgaria with 63 (15.7%) deaths. three hundred serum samples from 214 patients who had contracted the disease from 1957 to 1986 were investigated by indirect fluorescent antibody test (if-at). as antigen vero-e6 cells infected with the asian hantaan virus were used, as well as lung sections from bank voles (clethrionomys glareolus) infected with strains udmurt and kazan 6-cg from the eur ...19882902774
[persistent infection caused by hemorrhagic fever with renal syndrome virus in bank voles (clethrionomys glareolus)--the natural host of the virus]. 19872907924
[ectoparasites and phoresants of the root vole (microtus oeconomus pall.) in the polesye].drainage melioration in the polesye resulted in a sharp increase in the number of tundra vole (microtus oeconomus pall.) which quickly included into the parasitocenosis of the drained land. there were found 26 parasitic and nonparasitic species of gamasoidea, 3 species of ixodidae and 2 species of trombiculidae, 10 species of aphaniptera, 3 species of anoplura but there was found no species specific only for the tundra vole. all found parasites occur on many hosts. this explains wide parasitic l ...20102976144
photoperiodic modulation of testicular lh receptors in the bank vole (clethrionomys glareolus).the temporal changes in testicular binding of 125i-labelled hcg in juvenile bank voles (18 days of age, born and reared in a 18l:6d photoperiod) exposed to a long (18l:6d, group l) or short (6l:18d, group s) photoperiod for 0, 3, 7, 14 and 42-56 days were investigated. during testicular maturation, in group l, there was a slight initial decrease in lh receptor numbers per testis followed by a marked prepubertal rise during the initial phase of rapid testicular growth after which a decrease took ...19852999384
concordant divergence in proteins and mitochondrial dna between two vole species in the genus clethrionomys.the bank vole (clethrionomys glareolus) and the northern red-backed vole (c. rutilus) are two closely related species where interspecific crosses result in fertile female but sterile male offspring. mitochondrial dna (mtdna) from c. rutilus has passed the species barrier and is found in c. glareolus from northern fennoscandia. the present report shows that the genetic distance between the two species, calculated from enzyme data (nei's d), is 0.64. isoelectric focusing of muscle proteins resolve ...19883044353
seasonal acclimation of bank voles and wood mice: nonshivering thermogenesis and thermogenic properties of brown adipose tissue mitochondria.seasonal acclimation of nonshivering thermogenesis and brown adipose tissue was studied in wild bank voles (clethrionomys glareolus), yellow necked field mice and wood mice (apodemus flavicollis, a. sylvaticus). both, voles and mice increased their capacity for nonshivering thermogenesis during winter. thermogenic properties of brown fat (cytochrome c oxidase activity, mitochondrial protein content, gdp-binding of brown fat mitochondria) showed similar changes during seasonal acclimation; clethr ...19883049708
effect of differential photoperiod treatment on leydig cell ultrastructure in the bank vole (clethrionomys glareolus, s.).juvenile bank voles (18-22 days of age) born and reared in a stimulatory long photoperiod (18l:6d, lights on 0600-2400 hr) were subjected either to a long photoperiod (18l:6d, group l) or to a short photoperiod (6l:18d, lights on 0800-1400 hr, group s) for 6 to 8 weeks whereafter the animals were killed by decapitation. possible photoperiod-induced changes in leydig cell ultrastructure were studied by conventional transmission electron microscopy and stereological methods. striking differences i ...19883060394
changes in naloxone and haloperidol effects during the development of captivity-induced jumping stereotypy in bank voles.captivity-induced stereotypies in bank voles (clethrionomys glareolus) were inhibited at the age of 1, 2, 4, 6, 8 and 10 months by 0.2 mg/kg haloperidol s.c., while 35 mg/kg naloxone s.c. only inhibited them at the age of 4-6 months. other activities were not significantly inhibited. the change in the naloxone effect was not due to desensitization as animals treated only at 6 or 8 months reacted similarly. it is suggested that the expression of such stereotypies is influenced by endogeneous brai ...19883063550
[first signs of radioadaptation in the population of bank voles (clethrionomys glareolus) in the radiation biogeocenosis]. 20113069414
ovarian activity in outdoor and laboratory bred bank vole (clethrionomys glareolus) females. 19883069503
hemorrhagic fever with renal syndrome in czechoslovakia: detection of antigen in small terrestrial mammals and specific serum antibodies in man.a direct elisa technique was used for demonstration of hfrs virus antigen in suspensions of the lungs obtained from 774 wild small mammals belonging to 13 zoological species trapped in 21 localities in czechoslovakia between 1983 and 1984. western serotype of hfrs virus was demonstrated in the lungs of 8 microtus arvalis voles caught in the areas of central bohemia, southern moravia and western slovakia, and in one clethrionomys glareolus vole from the area of eastern slovakia. the eastern serot ...19863084630
transfer of mitochondrial dna from the northern red-backed vole (clethrionomys rutilus) to the bank vole (c. glareolus).using a silver staining method to detect dna fragments produced by restriction enzymes, it was possible to compare mitochondrial dnas (mtdnas) from 85 individuals of the bank vole (clethrionomys glareolus) trapped at 25 localities in fennoscandia. there are two distinctly different mtdna lineages, one occurring in southern and central fennoscandia and the other in the northern parts. a fragment comparison method shows about 12.7% nucleotide sequence divergence between these two lineages. this ma ...19873106637
[use of monoclonal antibodies for differentiating strains of the virus causing the hemorrhagic fever with renal syndrome].using monoclonal antibody and animal immune sera the experiment confirmed the existence of antigenic relationships between the strains of virus of hemorrhagic fever with renal syndrome (hfrs) isolated in the ussr from clethrionomys glareolus and members of all known serotypes of hfrs virus. clear-cut differentiation was made from serotypes apodemus and rattus, and cross-relationships were shown between the strains isolated in the ussr and prospect hill virus (phv), a member of the serotype micro ...19873124351
first isolations of borrelia burgdorferi from rodents collected in northern europe.spirochetes were found in 13% of ixodes ricinus collected from an island, near stockholm where human borreliosis is endemic. borrelia burgdorferi was cultivated from the kidney and/or spleen of wild rodents (clethrionomys glareolus and apodemus flavicollis) from the same island. spirochetes were identified as borrelia burgdorferi by indirect immunofluorescence assays using species and genus specific monoclonal antibodies. in these tests the spirochetes could not be differentiated from strains pr ...19883190915
effect of lead on postnatal development of the bank vole (clethrionomys glareolus). 19883288128
stereotyped behaviour, hyperaggressiveness and "tyrannic" hierarchy induced in bank voles (clethrionomys glareolus) by a restricted cage milieu.this investigation was originally conceived as a further development of studies made with the "amphetamine model" of psychosis. since amphetamine itself seemed to be the most important flaw in this model, it was judged promising to study abnormal behaviour in animals elicited without the use of drugs. bank voles were placed in two different cage milieux, a restricted milieu approaching usual laboratory conditions for experimental animals, and an enriched milieu offering opportunities for satisfa ...19873299497
[changes in the synthesis of the virion antigen of the tick-borne encephalitis virus after passage through ixodid ticks and small mammals].large-plaque strains of tick-borne encephalitis (tbe) virus possess a high peripheral activity, and in acute infection in continuous pig embryo kidney cells (pek) synthesize a virion antigen (va) whose subpopulations differ in their mobility in agarose gel electrophoresis: the bulk portion of va moves towards the cathode and a small one towards the anode. after long-term passages of these strains in ixodid ticks they lose their peripheral activity and produce only small plaques. in reproduction ...19873318134
seasonal effects on sexual maturation of male bank voles (clethrionomys glareolus).the weights of the testes and accessory glands, and the degree of morphological and functional development of the seminiferous tubules, were used as indicators of sexual maturation in male bank voles. males reared under constant laboratory conditions showed a significant relationship between the seasons and sexual maturation. young males reached maturity most rapidly in the reproduction season (mid-april to mid-october), while adult males matured earlier, in the spring season (mid-january to mid ...20123511257
[intermediate hosts of echinococcus multilocularis in northeastern france. description of lesions found in 3 naturally infested rodent species].the authors describe lesions of echinococcus multilocularis larvae found in one of 476 clethrionomys glareolus and one of 16 arvicola terrestris examined from lorraine and in four microtus arvalis from jura. the receptivity of each of these host-species is discussed.19863524402
[external structure of cuticle injuries and larval mouth parts of the chiggers euschoengastia rotundata and neotrombicula vulgaris (trombiculidae)].when e. rotundata larvae parasitize the abdominal part of the body of their host (clethrionomys glareolus), capsules with round terminal opening are formed from which hind part of the mite's body projects forwards. organization of the capsules shows that their walls are formed by a substance (probably by larval saliva) which differs from host's tissues. at the bottom of the capsules there are larval adhesive sites with openings in the proximal parts of stylostomes which resemble in their structu ...20073528991
analysis of interspecific relationships in mesostigmatid mites (acari: mesostigmata) in the nests of the bank vole (clethrionomys glareolus (schreber, 1780)).an analysis is presented of a community of mesostigmatid mites from the nests of clethrionomys glareolus found in the forest biotopes of vsetínské beskydy mts. (north moravia) throughout all seasons of the year. main attention was paid to the structure of the nest community, relationships of mites to their host and interspecific relationships in the mites themselves. primarily cluster and factor analyses based on correlation matrix were used for mathematical assessment. the particular elements o ...19863530922
seasonal and age distributions of babesia, hepatozoon, trypanosoma and grahamella species in clethrionomys glareolus and apodemus sylvaticus populations.four common blood parasites belonging to the genera babesia, hepatozoon, trypanosoma and grahamella were detected in sympatric populations of clethrionomys glareolus and apodemus sylvaticus. seasonal changes in the abundance of hosts and parasites were monitored in a longitudinal field survey for 18 months. all parasites in both hosts showed similar seasonal patterns with most transmission occurring in summer and autumn and ceasing in winter. amongst aged subsets of each host population, hepatoz ...19863537923
puberty delay of bank vole females in a high-density population.the onset of puberty in bank vole females was studied, with uterine weight, ovarian weight, and the number of large ovarian follicles used as indicators of gonadal activity. maturation of females born at the beginning of the reproductive season was suppressed by the presence of other females. puberty of animals born at the end of season was primarily influenced by climatic variation.19863539213
seasonal variations of testicular activity in juvenile and mature bank voles (clethrionomys glareolus). 19863545920
hormonal requirements and the influence of age on the decidual cell reaction in the bank vole (clethrionomys glareolus).laboratory bred bank voles of different age and reproductive status were bilaterally ovariectomized, treated with exogenous oestrogen or progesterone or both, and given an artificial decidual stimulus (crushing with artery forceps). an hormonal regimen of oestrogen (100 ng) followed by progesterone (1 mg) and small amounts of oestrogen (10 ng) was necessary for maximal development of the decidual cell reaction (dcr). a dcr did not develop if the animals were treated with only oestrogen or proges ...19873553583
the effect of interference in catecholamine biosynthesis on captivity-induced jumping stereotypy in bank voles (clethrionomys glareolus).the level of the captivity-induced jumping stereotypy in bank voles was significantly decreased by 150 and 200 mg/kg alpha-methyl-para-tyrosine and increased by 200 mg/kg l-dopa while 20 and 40 mg/kg fusaric acid had no effect. none of the other movements were affected by these drugs (as measured by a general activity meter), except for a nonsignificant decrease after treatment with fusaric acid. these results suggest that cerebral dopaminergic neuronal systems and not noradrenergic neuronal sys ...19873555375
propagation of nephropathia epidemica virus in mongolian gerbils.nephropathia epidemica virus, the etiological agent of hemorrhagic fever with renal syndrome in scandinavia, was serially propagated in mongolian gerbils (meriones unguiculatus). intracerebrally inoculated suckling gerbils developed subclinical infections, with viral antigen found in lung, brain, liver and spleen. the distribution of viral antigen was similar to that seen in experimentally infected bank voles (clethrionomys glareolus), the principal wild rodent reservoir of nephropathia epidemic ...19853882997
androgen-dependent proteins in the urine of bank voles (clethrionomys glareolus).the concentration of proteins in the urine of adult bank vole males was higher than that in urine of immature males and females. after separation of the urine by polyacrylamide gel electrophoresis in the presence of sds, the principal urinary protein had a slightly lower mobility than cytochrome c. urine from females or castrated males contained only trace amounts of this protein. injection of testosterone into castrated males increased this protein band. we suggest that bank vole males, like th ...19853897526
photoperiod-induced changes in the testicular metabolism of [4-14c]17 alpha-hydroxyprogesterone in the bank vole (clethrionomys glareolus).minces of the testes of bank voles, born and reared in a long (18l:6d) photoperiod until weaning (18-22 days of age) and subjected thereafter to a short (6l:18d, group s) or a long (18l:6d, group l) photoperiod for 6-9 weeks, were incubated with [4-14c]17 alpha-hydroxyprogesterone in the presence of cofactors (nadp/nadph, 1.3 mmol/1) for 1 h at 37 degrees c. the radioactive metabolites were characterized and identified by thin-layer chromatography with derivative formation and chromatography to ...20143900383
reservoir animals for nephropathia epidemica in norway: indications of a major role for the bank vole (c. glareolus) in comparison with the woodmouse (a. sylvaticus).small rodents were collected live in two different locations within a nephropathia epidemica (ne) endemic area, and tested for both antiviral serum antibodies and viral antigens in lung sections. in one location, only apodemus sylvaticus (woodmice) were found in the traps, in the other, both a. sylvaticus and clethrionomys glareolus (bank voles) were collected. among the woodmice from the former location the prevalence of ne virus markers was significantly lower than for either woodmice or bank ...19853919085
definition of three serotypes of hantaviruses by a double sandwich elisa with biotin-avidin amplification system.a total of seven different isolates of hantaviruses from five mammalian species and six geographical areas were examined by a double sandwich elisa with biotin-avidin amplification system. three serotypes were recognized by comparing antigen titres to homologous and heterologous antibody and blocking antibody titres to homologous and heterologous antigens. serotype 1 included four strains: hantaan virus isolated from apodemus agrarius in south korea, hupei-i isolated from acute-phase serum from ...19853926952
[cytochemical differentiation of t- and b-lymphocytes in the short-tailed mouse (clethrionomys glareolus)].using the cytochemical demonstration of alkaline phosphatase (ap) two different populations of lymphocytes are seen in the short-tailed mouse clethrionomys glareolus: ap-positive and pa-negative lymphocytes. the aim of this study was to find out as to whether in this species ap represents a cytochemical marker for differentiating t- and b-lymphocytes. for this purpose, we analysed the frequency of ap-positive lymphocytes in the peripheral blood, bone marrow, thymus, lymph node, spleen, and peyer ...19853927411
brucella abortus infection in the bank vole (clethrionomys glareolus). 19853928085
[differentiation of different lymphocytes by alkaline phosphatase--the cytochemical parameter of alkaline phosphatase in relation to t- and b-lymphocytes in clethrionomys glareolus (author's transl)]. 19734142198
[relation of clethrionomys glareolus schr. and apodemus flavicollis melch. to the virus of tick-borne encephalitis in 2 different biotopes]. 19664224556
the role of spacing behavior among females in the regulation of reproduction in the bank vole. 19734522393
interspecific relationships of gamasoid mites in the nests of clethrionomys glareolus. 19724670810
the development of the ovary from birth to maturity in the bank vole (clethrionomys glareolus) and the vole (microtus agrestis). 19744829082
the protective effect in bank voles of some strains of bcg.the bank vole, like the guinea-pig, can be immunized against tuberculosis with very small doses of bcg, presumably because bcg is sufficiently virulent for both these species to multiply freely until the immunization of the animal has reached a maximum. large doses of bcg induce an earlier, but not an ultimately stronger, immunity. however, the smallest immunizing dose is not the same for all bcg strains and this is a feature that may be used to differentiate strains. in the studies reported by ...19704921094
observations on the use of the exercise-wheel in relation to the social rank and hormonal conditions in the bank vole (clethrionomys glareolus), and the norway lemming (lemmus lemmus). 19715166538
the autecology of small rodents and insectivores of the tribec mountain range.small rodents and insectivores have become the main reservoirs of tick-borne encephalitis virus. in order, therefore, to demonstrate the dynamic and structural changes occurring in a natural focus of tick-borne encephalitis, information has been collected on the distribution, habitat, life-cycle, population density and extent of tick infestation of the rodents and insectivores that inhabit the tribec region and adjacent parts of the hronský inovec mountains. the following mammals are discussed: ...19675298539
the role of small rodents and hedgehogs in a natural focus of tick-borne encephalitis.the role of small rodents and hedgehogs in the circulation of tick-borne encephalitis in natural foci in the tribec region of czechoslovakia has been studied. isolation of virus from the blood of apodemus flavicollis, clethrionomys glareolus and erinaceus roumanicus, as well as the demonstration of antibodies in the sera of these mammals and of a. sylvaticus, microtus arvalis and sciurus vulgaris, showed that these mammals had been in contact with the virus in recent years. the proportion of pos ...19675298543
the morphology and biology of trypanosoma (herpetosoma) evotomys of the bank-vole, clethrionomys glareolus. 19695357322
[study of antihemagglutinin formation in clethrionomys glareolus and cl. rutilus experimentally infected with tick-borne encephalitis virus]. 19695373445
genetic studies of the amylase isoenzymes of the bank vole, clethrionomys glareola. 19695399207
[viremia in clethrionomys glareolus, clethrionomys rutilis and stenocranius gregalis experimentally infected with tick-borne encephalitis virus]. 19695400212
ovulation in the bank vole, clethrionomys glareolus. 19705492035
[biometry and reproduction biology of the red field mouse clethrionomys glareolus (schreber, 1780) at different altitudes in switzerland]. 19705517259
the fine structure of extraocular muscles of the bank vole, clethrionomys glareolus schr. 19705523620
seasonal changes in the adenohypophysis of the bank vole, clethrionomys glareolus. 19715558400
classification of muscle fibers in the extraocular muscles of the bank vole (clethrionomys glareolus, schr.) and tree sparrow (passer montanus, l.). 19715562653
seasonal variability of the leukocyte count in the bank vole clethrionomys glareolus (schreber, 1870). 19675584793
variability of the leukocyte count in the blood of bank vole, clethrionomys glareolus (schreber, 1870) under laboratory conditions. 19675584794
experimental infection with tick-borne encephalitis virus in clethrionomys glareolus, apodemus flavicollis, apodemus sylvaticus and mus musculus. 1. virological studies. 19665958418
experimental infection with tick-borne virus in clethrionomys glareolus, apodemus flavicollis, apodemus sylvaticus and mus musculus. 2. serological studies. 19665958662
the nature of trichophyton persicolor infection in the bank vole and the interpretation of the results of sampling techniques. 19676048618
nephropathia epidemica: detection of antigen in bank voles and serologic diagnosis of human infection.an indirect immunofluorescence test for detection of serum antibodies specific for nephropathia epidemica (ne) has been developed with use of acetone-fixed cryostat sections of the lungs of bank voles (clethrionomys glareolus) that had been trapped from the ne-endemic area in finland as antigen. ne antigen was detected as distinct fluorescence in the cytoplasm of alveolar and macrophage-like cells. the 16 patients studied included typical cases from an endemic area, cases from a family outbreak, ...19806102587
[studies of the ecology of some blood protozoa of wild small mammals in north germany (author's transl)].in 1977 and 1978, 696 small mammals of 9 different species were surveyed for protozoal blood parasites. the following parasites were found: hepatozoon sylvatici in apodemus flavicollis, h. erhardovae and trypanosoma evotomys in clethrionomys glareolus, t. microti and babesia microti in microtus agrestis and t. croicidurae in crocidura russula. h. erhardovae showed regularly high infection-rates (more than 70%) during all the years in 1977 and 1978. h. sylvatici was found only in 5.6% of the yell ...19806104423
electron microscopy of rabbit embryo fibroblasts infected with herpesvirus isolates from clethrionomys glareolus and apodemus flavicollis.electron microscopy of rabbit embryo fibroblasts infected with five herpesvirus isolates from murine rodents (clethrionomys glareolus and apodemus flavicollis) revealed morphological changes characterized by the formation of intranuclear vacuoles, in which envelopment of nucleocapsids took place. the herpesvirus nucleocapsids were formed in the vicinity of intranuclear granular inclusions. margination of chromatin, disaggregation of nucleoli and, in some isolates, the occurrence of tubular struc ...19816113749
[detection of the etiologic agent of hemorrhagic fever with a renal syndrome in the lungs of wild rodents].the results of the studies on wild rodents trapped in the areas of the leningrad region visits to which were associated with human cases of hemorrhagic fever with renal syndrome (hfrs) are presented. in one of these areas 30% of bank voles were found to be infected. specific fluorescence of high intensity was found in ctyostat sections of lungs from bank voles treated by the indirect fluorescent antibody procedure. fluorescent granules, their clumps and large conglomerates were localized in the ...19816115516
experimental characteristics of viraemia caused by two strains of tick-borne encephalitis virus in small rodents.two strains of tick-borne encephalitis (tbe) virus differing in virulence for subcutaneously (s.x.) inoculated adult white mice elicited different levels of viraemia in small rodents. strain skalica of a lowered virulence caused no detectable viraemia in adult clethrionomys glareolus and only negligible viraemia in adult apodemus sylvaticus, microtus arvalis and pitymys subterraneus species. the virulent strain 204 gave rise to higher levels of viraemia, sufficient for infection of tbe virus vec ...19816116416
detection of hemorrhagic fever with renal syndrome (hfrs) virus in the lungs of bank voles clethrionomys glareolus and redbacked voles clethrionomys rutilus trapped in hfrs foci in the european part of u.s.s.r., and serodiagnosis of this infection in man.the antigen of hfrs virus was demonstrated by means of the indirect fluorescent antibody procedure in the lung tissue of bank and redbacked voles (clethrionomys glareolus, cl. rutilus) trapped in hfrs foci in the european part of ussr. this antigen has been used satisfactorily for serodiagnosis of hfrs in several european and asian regions of the ussr where hfrs had been found to be endemic.19816117265
[demonstration of the virus of hemorrhagic fever with renal syndrome in the organs of rodents from natural foci of the infection in the ussr and the serodiagnosis of the diseases].an indirect fluorescent antibody test (ifat) and an enzyme-immunological test (elisa) were used for the detection of hfrs virus in organs of rodents from hfrs foci in the ussr. the virus was found in 115 out of 1120 bank voles, 9 out of 92 redbacked voles, and 2 field voles examined. spontaneous infection-rate of bank voles in population varied from 1.3 to 100% correlating with the epidemiological situation in foci. ifat and elisa were successfully used for serodiagnosis of hfrs. examinations of ...19816121425
indirect immunofluorescence tests in korean hemorrhagic fever and epidemic (endemic) nephropathia: treatment at low ph for removal of 'nonspecific' fluorescence in tissues from immunocompetent hosts.a 'nonspecific' fluorescence probably caused by immunocomplexes appears in the lung tissue of immunocompetent hosts infected with korean hemorrhagic fever (khf) or epidemic nephropathy (en) viruses. indirect immunofluorescence tests with such khf- or en-infected tissue as antigen are unsatisfactory for the detection of low-titer antibody or the reading of titer endpoints. treatment of acetone-fixed lung sections at ph 3.0 followed by washing with 0.01 m phosphate-buffered saline and distilled wa ...19826126464
growth characteristics of herpesviruses isolated from free living small rodents.sixteen cell cultures of different origin (bird, rodents, carnivores, pigs, monkey, man) reproduced five strains of herpesviruses isolated from small free living rodents apodemus flavicollis and clethrionomys glareolus revealing cytopathic changes typical for members of the family herpesviridae. the virus titres in different cell cultures ranged from 10(2) to 10(7) tcd50 per ml. the growth curves of two isolates originating from both animal species resembled to those obtained with human herpesvi ...19826127933
nephropathia epidemica in norway: description of serological response in human disease and implication of rodent reservoirs.in search for the norwegian nephropathia epidemica (ne) agent, lung sections from small rodents and sera from human patients have been tested by indirect fluorescent antibody technique (ifat). a cytoplasmatic antigen reacting with patient sera and a korean hemorrhagic fever (khf) reference antiserum was found in the lungs of 6 bank voles (clethrionomys glareolus) from 2 different locations. antibodies reacting with vole lung antigen (ne agent) and khf agent in a-549 cells were detected in sera f ...19836133345
nephropathia epidemica in norway: antigen and antibodies in rodent reservoirs and antibodies in selected human populations.nephropathia epidemica (ne) antigen was detected by ifat (indirect fluorescent antibody technique) in the lungs of 14 of 97 bank voles (clethrionomys glareolus) collected in three endemic areas. the distribution of antigen positive voles within an endemic location was scattered. antibodies to korean hemorrhagic fever (khf) virus antigens were detected by ifat in 12 of 14 ne antigen positive bank voles and in 15 of 83 that were antigen negative. ne antigen positive voles exhibited higher antibody ...19846146649
experimental pathogenesis of murine herpesvirus in newborn mice.newborn white mice were susceptible to peroral (p.o.) infection with murine alphaherpesvirus isolated from free-living clethrionomys glareolus. death occurred within 6-8 days in animals infected with the higher virus dose of 4.8 log10 tcid50. clinical symptoms also occurred in some animals infected with lower doses, while others developed inapparent infection as judged by presence of humoral antibodies at 60 days post-infection (p.i.). the virus was detected in the lungs, blood, liver, spleen, k ...19846147999
[transmission and course of parasitemia of babesia microti (hannover i strain) in the bank vole (clethrionomys glareolus) and field vole (microtus agrestis)].in germany only m. agrestis has been found to be naturally infected with b. microti while in other european countries several rodent species were found as mammalian hosts. in the laboratory c. glareolus got easily infected by german strains of b. microti, if parasitized blood was inoculated. but results of this method are not transferable to natural conditions. therefore this study was carried out. it was to prove whether c. glareolus is susceptible to b. microti transmitted by nymphs of i. rici ...19846150621
haemorrhagic fever virus with renal syndrome in small rodents in czechoslovakia.the antigen of haemorrhagic fever with renal syndrome (hfrs) was detected in the lungs of the following free-living small rodents trapped in different localities in eastern slovakia: clethrionomys glareolus (2 positive samples of 7), apodemus flavicollis (1 sample of 24) and apodemus agrarius (7 positive samples of 66). the virus was first identified by indirect fluorescent antibody (fa) staining using human convalescent serum from a case of epidemic nephropathy (ne) of scandinavia. the lung sus ...19846151356
clinical and serological diagnosis of nephropathia epidemica, the mild type of haemorrhagic fever with renal syndrome.the serological diagnosis of nephropathia epidemica (ne), the mild european type of haemorrhagic fever with renal syndrome (hfrs), was studied by means of an indirect immunofluorescent antibody technique (ifat) in a large outbreak in finland (morbidity 1.4/1000 population). acetone-fixed sections of lung of bank voles (clethrionomys glareolus) naturally infected with puumala virus served as antigen. altogether 65 patients were followed serologically for up to 20 months. serological results obtai ...19846151960
isolation and characterization of alpha-amylase messenger rna from bank vole parotid glands. evidence for two separate messenger rnas coding for amylase and an amylase-related protein.bank vole saliva contains two glycogen-precipitable proteins, both of which show affinity for the alpha-amylase inhibitor cycloheptaamylose. one of these proteins, amylase, has a molecular weight of 55,000, judged from dodecylsulphate/acrylamide gel electrophoresis. the other has an apparent molecular weight of 59,000 and has no amylase activity. we report here that tryptic peptide maps as well as amino-acid composition analyses indicate extensive homology between the two proteins. we have also ...19816167442
antigenic relatedness of alphaherpesviruses isolated from free-living rodents.complement fixation and virus neutralization tests confirmed that alphaherpesviruses isolated from free-living apodemus flavicollis and clethrionomys glareolus rodents form an antigenically identical or very close group. immunofluorescence showed that antigen assembly and distribution within the infected cell resembles that of members of the alphaherpesvirinae subfamily. radioimmunoassay revealed close antigenic relatedness between five rodent herpesvirus isolates. moreover it suggested a possib ...19826188352
isolation and propagation of nephropathia epidemica virus in bank voles.three strains of nephropathia epidemica (ne) virus were isolated from lung tissues of bank voles (clethrionomys glareolus) and a grey-sided vole (c. rufocanus) trapped in västerbotten county, sweden. two of these isolates were serially passaged in seronegative laboratory-bred bank voles. experimentally infected animals developed a subclinical infection characterized by virus persistence, particularly in lung tissue. attempts to infect other species of colonized rodents with ne virus and to isola ...19846208601
effect of photoperiod on the testicular steroidogenesis of the bank vole (clethrionomys glareolus, schreber): an in vitro study. 19826213444
features of circulation of hemorrhagic fever with renal syndrome (hfrs) virus among small mammals in the european u.s.s.r.the use of indirect fluorescent antibody testing (ifat) and enzyme linked immunosorbant assay (elisa) procedures allowed the hemorrhagic fever with renal syndrome (hfrs) virus antigen to be detected not only in the known reservoir host, clethrionomys glareolus, but also in 7 other species of small mammals in european foci of the u.s.s.r. marked viscerotropism of hfrs virus and the participation of brown fat in maintaining the infection in rodents were demonstrated. the frequency of detection of ...19836220688
testicular steroid metabolism in juvenile bank voles (clethrionomys glareolus schreber) exposed to different photoperiods: an in vitro study.juvenile male bank voles (18-22 days of age) were either sacrificed immediately (group c) or subjected first to a long (18l:6d, lights on 0600-2400; group l) or a short (6l:18d, lights on 0800-1400; group s) photoperiod for 1 week. the animal were killed by decapitation, the gonads were excised, and minced, and the conversion of [4-14c]pregnenolone (delta 3p) and [4-14c]dehydroepiandrosterone (dha) to metabolites was studied in vitro. the radioactive steroids formed were separated and identified ...19836226556
temporal changes in testicular histology and steroidogenesis in juvenile bank voles (clethrionomys glareolus, schreber) subjected to different photoperiods. 19836339204
fertility of the post-partum bank vole (clethrionomys glareolus).analysis of records of a bank vole breeding colony suggests that fertility is high immediately post partum, declines during established lactation and rises after weaning of young. mating tests with lactating females and females whose young had been removed at birth showed that receptivity is reduced during lactation, although amongst the females which did mate there was no difference between lactating and non-lactating animals in the proportion which produced litters. however, average size of li ...19836341576
ultrastructural studies on the thymus gland after the administration of phenylhydrazine to bank voles (clethrionomys glareolus).the thymus glands of wild and laboratory reared bank voles (clethrionomys glareolus) were examined for ultrastructural changes before and after the administration of low (20 mg/kg) and high (60 mg/kg) doses of phenylhydrazine (phz) given intraperitoneally on days 0, 1 and 3 of a dosing regime. erythrocytes developed in focal aggregations in the subcapsular and outer cortical zones. there was a marked depletion of the subcapsular cortex following drug treatment that was reversed with time. a larg ...19836349818
influence of sexual experience and social environment on fertility and incidence of mating in young female bank voles (clethrionomys glareolus).between 30 and 40 days of age, female bank voles were kept singly, in female pairs, separated from an adult male by a wire mesh, or paired with a vasectomized male. at day 40 they were paired with adult intact males. fertility at the first mating was low (22-25%), but if the females had previously mated with the vasectomized male fertility of the subsequent mating with the intact male was significantly increased (63%). sterile matings therefore had a priming effect on the females, and could be i ...19836350570
activity in the corpora lutea resulting from sterile matings in clethrionomys glareolus: a histochemical study.the capacity for steroid production in the corpora lutea (cl) of bank voles was examined by enzyme histochemistry for 3 beta-hydroxysteroid dehydrogenase. the results revealed similar activity in the cl resulting from sterile matings and the cl of pregnancy 2 days after mating. six days after mating, the cl of pregnancy showed a strong reaction, while there was almost no reaction in the cl from sterile matings, which were clearly regressed at this time. it is suggested that the cl from sterile m ...19836355353
[data on the distribution and ecology of ixodes trianguliceps (ixodidae) in kharkov province].in the ranges of the kharkov district ixodes trianguliceps bir. is distributed in the limits of forest-steppe zone from the western border to the valley of the seversky donets. through the forests along the seversky donets the species gets to the steppe zone. most abundant the species is in aspen groves, its main host -- clethrionomys glareolus schreb. the peak of abundance was recorded in june.20076359025
identification of a reservoir of leptospira interrogans serovar muenchen in voles (microtus agrestis and clethrionomys glareolus) in england.infection with leptospires of the australis serogroup is widespread in voles in southern england and the serovar identification of five randomly-selected isolates was determined by cross agglutination absorption. two isolates from short-tailed voles (microtus agrestis) and three isolates from bank voles (clethrionomys glareolus) were all identified as serovar muenchen. the only previous isolation of this serovar is that from a human in germany.19836372316
influence of age and artificial vaginal stimulation on fertility in female bank voles (clethrionomys glareolus).female bank voles mated when 30-50 days old showed only 20-25% fertility at the first mating, but fertility increased to about 80% in animals greater than 100 days of age at the first mating. young females subjected to artificial cervical stimulation after the completion of a normal mating showed a much higher fertility than normally mated controls (76 compared with 29%). the reason for the low fertility therefore appears to be that young females require more stimulation to activate the corpora ...19846374130
[detection of the larva of taenia multilocularis in 2 voles (microtus arvalis and clethrionomys glareolus) in a focus of alveolar echinococcosis in the massif central (france)].small wild rodents were collected and examined during 1983-1984 to investigate parasite-host relationships of echinococcus multilocularis in one habitat suitable for red foxes of auvergne (france). the natural infestation of the liver of two different voles, microtus arvalis and clethrionomys glareolus, by the larval stage of the cestode is recorded for the first time. in the both cases, the larvae were fully developed and numerous protoscolices were present with some calcareous corpuscules; the ...19846440675
isolation in vero-e6 cells of hanta virus from clethrionomys glareolus captured in the bashkiria area of the u.s.s.r. 19846442126
[tissue radiosensitivity of bank voles living under different ecological conditions]. 20066444748
[lice (anoplura) of small mammals in the volga-kama preserve].18 068 small mammals belonging to 15 species were examined, 25 059 lice of 7 species (hoplopleura acanthopus, h. edentula, h. affinis, h. longula, polyplax serrata, p. borealis, neohaematopinus laeviusculus) were collected from 2355 animals of 14 species. information is given on the species composition, occurrence and abundance for lice on shrews and rodents. 50 per cent of collected animals belong to clethrionomys glareolus and about 85 per cent of lice--to hoplopleura edentula. frequent cases ...19816452609
[differentiation of tick-borne encephalitis virus strains according to the levels of viremia produced in bank voles]. 19816457974
antibodies to berne virus in horses and other animals.after inoculation into 2 foals, berne virus induced neutralizing antibody, but did not cause clinical symptoms. in a horizontal study of seropositive mares and their offspring, a decline of maternal antibodies and a sudden synchronous seroconversion in all foals were observed, again without clinical symptoms. the virus is widespread in the swiss horse population and has been so during the last decade; rises in antibody titers were noted in 9% of paired sera sampled at random. positive reactions ...19846506447
serovar identification of leptospires of the australis serogroup isolated from free-living and domestic species in the united kingdom.eighteen isolates from the australis serogroup from free-living and domestic animals were identified using the cross agglutination absorption test. serovar muenchen was found only in england and wales in wood mice, short tailed and bank voles, a grey squirrel and a pig. serovar bratislava was found in hedgehogs in england, wales and northern ireland and also in a brown rat from northern ireland. serovar bratislava was isolated from sheep in both england and northern ireland and from horses in no ...19836622847
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