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[incidence and control of the american giant liver fluke, fascioloides magna, in a population of wild ungulates in the danubian wetlands east of vienna].fascioloides magna,the giant liver fluke, is an introduced parasite imported to europe with infected game which is endemic in the austrian wetlands of the danube from vienna to the slovakian border. due to its pathogenicity (especially in roe deer) and its potential of transmission to domestic ruminants a project on the epidemiology and control of this parasite was carried out between 2000 and 2005. to assess distribution faecal droppings from red deer were collected in an area along the danube ...200617009716
the giant liver fluke fascioloides magna (bassi 1875) in cervids in the czech republic and potential of its spreading to germany.the giant liver fluke fascioloides magna is an important parasite of cervids in europe. from september 2003 to december 2005, faecal samples and livers of red deer (cervus elaphus) and fallow deer (dama dama) were investigated to determine the current distribution of the fluke in the czech republic. faecal samples were collected from 20 different areas, and livers of hunted deer were dissected from each locality to confirm f. magna infection. the prevalence of f. magna in examined areas determin ...200717016724
dna-based identification of a hepatic trematode in an elk calf.liver fluke infection was identified as a probable cause of clinical disease in an approximately 6-mo-old elk (cervus elaphus) in coastal oregon. clinical pathology and necropsy findings are described. the alcohol-fixed flukes that were submitted for identification were similar in size to fasciola hepatica, but their shape resembled fascioloides magna in that they lacked a distinctive anterior cone. a few structures consistent with the eggs of f. magna were observed in liver lesions, suggesting ...200717984277
humoral immune responses during experimental infection with fascioloides magna and fasciola hepatica in goats and comparison of their excretory/secretory products.this study investigated the humoral immune responses of goats experimentally infected with fascioloides magna and fasciola hepatica to f. magna excretory/secretory products (fmesp) or f. hepatica excretory/secretory products (fhesp), respectively. an enzyme-linked immunosorbent assay (elisa) was used to determine serum antibody responses and for possible discrimination of f. magna and f. hepatica infections in goats. comparison of esps of both flukes and evaluation of esp antigenicity was also s ...200717265088
prevalence and intensity of infections in the lymnaeid snail omphiscola glabra experimentally infected with fasciola hepatica, fascioloides magna and paramphistomum daubneyi.single and double infections of juvenile omphiscola glabra (gastropoda: lymnaeidae) with paramphistomum daubneyi and/or fasciola hepatica were carried out to determine the redial burden and cercarial production in snails dissected at day 60 or at day 75 post-exposure (p.e.) in the laboratory at 20 degrees c. the results were compared with those obtained with single-miracidium infections by fascioloides magna. compared to f. hepatica, low values were noted at day 75 p.e. for the prevalence of sna ...200717381861
optimization of metacercarial production for three digenean species by the use of petri dishes for raising lettuce-fed galba truncatula.experimental infections of galba truncatula with fasciola hepatica, fascioloides magna, or paramphistomum daubneyi were carried out at 20 degrees c to determine if the use of 14-cm petri dishes for breeding lettuce-fed snails enhanced the characteristics of snail infections. compared to infected snails raised in boxes up to day 30 post-exposure and later in individual 35-mm dishes, the survival of g. truncatula kept in 14-cm dishes and the shell height of cercariae-shedding snails during the fir ...200717061111
sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke fascioloides magna (trematoda: fasciolidae): intraspecific variation and differentiation from fasciola hepatica.complete sequences of ribosomal and mitochondrial genes of the giant liver fluke fascioloides magna are presented. in particular, small subunit (18s) and internal transcribed spacers (its1 and its2) of the ribosomal gene (rdna), as well as cytochrome c oxidase subunit i (cox1) and nicotinamide dehydrogenase subunit i (nad1) of the mitochondrial dna (mtdna), were analyzed. the 18s and its sequences were compared with previously published sequences of the liver fluke fasciola hepatica. fixed inter ...200818372622
experimental infection of liver flukes, fasciola hepatica and fascioloides magna, in bison (bison bison).this experimental study was conducted to evaluate the susceptibility of american bison (bison bison) to liver flukes, fascioloides magna and fasciola hepatica. six bison were each experimentally inoculated with 600 metacercariae of fascioloides magna, and three were later treated with triclabendazole suspension at 40 mg/kg of body weight. four additional bison were each experimentally inoculated with 600 metacercariae of fasciola hepatica. five control bison were placebo controls. two controls a ...201020090045
development of high-resolution melting (hrm) analysis for population studies of fascioloides magna (trematoda: fasciolidae), the giant liver fluke of ruminants.the high-resolution melting (hrm) technique was successfully optimized as fast and effective method for population study of digenetic fluke, fascioloides magna (trematoda: fasciolidae), originally north american liver parasite of free-living and domestic ruminants. previously selected variable region (439 bp) of mitochondrial cytochrome c oxidase subunit i (cox1) of 249 fluke individuals from enzootic european and north american regions were sequenced and mutually compared. the sequence analysis ...201020967463
multiple origins of european populations of the giant liver fluke fascioloides magna (trematoda: fasciolidae), a liver parasite of ruminants.the giant liver fluke, fascioloides magna, a liver parasite of free-living and domestic ruminants of europe and north america, was analysed in order to determine the origin of european populations and to reveal the biogeography of this originally north american parasite on the european continent. the variable fragments of the mitochondrial cytochrome c oxidase subunit i (cox1; 384bp) and nicotinamide dehydrogenase subunit i (nad1; 405bp) were used. phylogenetic trees and haplotype networks were ...201021172350
an optimized dna extraction and multiplex pcr for the detection of fasciola sp. in lymnaeid snails.this study deals with the development and validation of an original pcr protocol to assess the presence of fasciola hepatica in galba truncatula its main intermediate host in western europe. in the present study two dna extraction techniques are compared and a new multiplex pcr is described. the chelex(®) dna extraction technique showed to be more appropriate than the classical phenol/chloroform/proteinase k based method because of the absence of toxic organic solvent, shorter duration and lower ...201021242033
a natural focus of the blood fluke orientobilharzia turkestanica (skrjabin, 1913) (trematoda: schistosomatidae) in red deer (cervus elaphus) in hungary.the large american liver fluke, fascioloides magna - introduced to europe before 1875 - was the first non-indigenous trematode to be detected in hungarian cervids in 1995. as the most precious deer population became infected in the gemenc game reserve a study has been launched to assess the extent and the effect of f. magna infections and to examine the options for the treatment of infected deer populations. livers of red deer shot during regular hunting were submitted for the survey and were in ...201020347227
liver enzymes and blood metabolites in a population of free-ranging red deer ( cervus elaphus) naturally infected with fascioloides magna.we investigated the effects of fascioloides magna infection on the serum biochemistry values of the naturally infected red deer population in eastern croatia. the investigation was performed on 47 red deer with f. magna infection confirmed patho-anatomically in 27 animals (57.4%). fibrous capsules and migratory lesions were found in 14 deer while only fibrous capsules without migratory lesions were found in 13 deer. in 13 deer both immature and mature flukes were found, in 5 deer only immature f ...201121729388
morphological characterization of adult fascioloides magna (trematoda: fasciolidae): first sem report.five adult fascioloides magna specimens were recovered from the livers of naturally infected calves from texas, usa. scanning electron microscopy was used to study the morphological characteristics of the trematodes. these mature flukes measured 35-100-ámm in length by 15-25-ámm in width and had an oval dorsoventrally flattened body, with no anterior cone. the tegument was armed with sharp spines. around the oral and ventral suckers, some of the spines were small, with a sharp point, while other ...201121842384
a comparative study of karyotypes and chromosomal location of rdna genes in important liver flukes fasciola hepatica and fascioloides magna (trematoda: fasciolidae).chromosomal characteristics, i.e., number, size, morphology, and location of ribosomal dna (rdna) clusters were examined in two medically important liver flukes, fasciola hepatica and fascioloides magna (fasciolidae), using conventional giemsa staining and fluorescent in situ hybridization (fish) with ribosomal 18s rdna probe. a comparison of f. magna and f. hepatica karyotypes confirmed significant differences in all chromosomal features. whilst the karyotype of f. hepatica comprised ten pairs ...201121509448
update on trematode infections in sheep.trematode parasites live in the liver, fore stomachs or blood vessels of a wide range of animals and humans. most of them have a special economic and veterinary significance. liver fluke disease of sheep and other animal species is caused by the common liver fluke fasciola hepatica. hepatic fasciolosis occurs throughout the world, where climatic conditions are suitable for the survival of aquatic intermediate host snails. also of importance for ruminants, in some parts of the world, are fasciola ...201222521973
recovery of fascioloides magna (digenea) population in spite of treatment programme? screening of galba truncatula (gastropoda, lymnaeidae) from lower austria.during the past decade, fascioloides magna, the large american liver fluke, has spread within free-living deer in wetlands of the danube in lower austria. the aim of this study was to determine the current infection rates with f. magna and other digenean parasites in the intermediate host snail galba truncatula from risk areas in lower austria. a total of 3444 g. truncatula were collected and examined microscopically for the presence of digenean trematodes. a set of randomly selected snails and ...201222381476
lymnaea fuscus (pfeiffer, 1821) as a potential intermediate host of fascioloides magna in europe.experimental infections of two different populations of lymnaea fuscus in france and sweden, with a czech isolate of fascioloides magna were carried out to determine if this lymnaeid species enables parasite larval development. species identification of both snail populations was performed using the morphology of the copulatory organ, and also confirmed by sequencing of the internal transcribed spacer 2 (its2) region of the snail genomic rdna. only juvenile snails measuring less than 3mm (1-3 we ...201222902745
radix spp.: identification of trematode intermediate hosts in the czech republic.lymnaeid snails of the genus radix serve as intermediate hosts of some schistosomes and fasciolids. in europe, delineation of species within the genus radix is unresolved and, therefore, spectrum of snail hosts susceptible to trematode infections is under discussion. we used and compared three criteria for species delineation using snails collected at 43 localities. (a) sequence analysis of its-2 rdna disclosed that the collected snails belong to four species - r. auricularia (linnaeus, 1758), r ...201222875675
a deep exploration of the transcriptome and "excretory/secretory" proteome of adult fascioloides magna.parasitic liver flukes of the family fasciolidae are responsible for major socioeconomic losses worldwide. however, at present, knowledge of the fundamental molecular biology of these organisms is scant. here, we characterize, for the first time, the transcriptome and secreted proteome of the adult stage of the "giant liver fluke," fascioloides magna, using illumina sequencing technology and one-dimensional sds-page and offgel protein electrophoresis, respectively. a total of ∼54,000,000 reads w ...201222899770
the role of hepatic myofibroblasts in liver cirrhosis in fallow deer (dama dama) naturally infected with giant liver fluke (fascioloides magna).this paper describes liver cirrhosis in 35 fallow deer infected with the giant liver fluke, as well as the distribution, origin, and role of myofibroblasts in its development.201323497565
lymnaea neotropica and lymnaea viatrix, potential intermediate hosts for fascioloides magna.experimental infections of two south american lymnaeid populations with fascioloides magna were carried out to determine whether these snails may sustain larval development of this digenean and, if so, to quantify their potential for cercarial production. the reference group was a french population of galba truncatula infected and raised according to the same protocol. according to the internal transcribed sequence (its)-1 segment of their genomic rdna, these south american populations were iden ...201323072755
the origin of the giant liver fluke, fascioloides magna (trematoda: fasciolidae) from croatia determined by high-resolution melting screening of mitochondrial cox1 haplotypes.the high-resolution melting (hrm) method, recently optimized as a reliable technique for population study of the european fascioloides magna populations, was applied to determine an origin of f. magna individuals from croatia. the structure and frequency of mitochondrial cytochrome c oxidase subunit i (439 bp; cox1) haplotypes of 200 croatian flukes coming from 19 red deer (cervus elaphus elaphus) livers were screened and compared with recently determined reference samples of f. magna from all e ...201323609601
phylogenetic data suggest the reclassification of fasciola jacksoni (digenea: fasciolidae) as fascioloides jacksoni comb. nov.fasciola jacksoni (cobbold, 1869) is a highly prevalent (18-62%) species colonizing the liver (less frequently the lungs, kidneys, pericardia, and intestines) of elephas maximus indicus and elephas maximus maximus in the indomalayan region, causing cirrhosis, hemorrhages, and connective tissue proliferation. the phylogenetic relationships of fasciola jacksoni in relation to representative species of the superfamily echinostomatoidea was assessed using four independent dna regions. the analysis i ...201323411741
highland cattle and radix labiata, the hosts of fascioloides magna.fascioloides magna is a pathogenic fluke introduced to europe ca 140 years ago. as it is spreading over the continent, new intermediate and definitive hosts might be involved in transmission of the parasite. in europe, several studies reported potential new intermediate snail hosts (radix spp.) for f. magna, and also several cases of fascioloidosis of wild and domestic animals were published. however, the data based on molecular and histological analyses confirming these findings remained unrepo ...201424517409
lymnaea cubensis, an experimental intermediate host for fascioloides magna.single-miracidium infections of lymnaea cubensis (pfeiffer) from guadeloupe with the giant liver fluke fascioloides magna (bassi, 1875) (digenea) were carried out during five successive snail generations to determine if this lymnaeid might sustain complete larval development of the parasite. controls were constituted by a french population of galba truncatula (miller) (a single generation) infected according to the same protocol. it was recorded that prevalence and intensity of f. magna infectio ...201424822325
wherefrom and whereabouts of an alien: the american liver fluke fascioloides magna in austria: an overview.the giant liver fluke fascioloides magna, an invasive species originating from north america, was recorded in austria in the wild for the first time in 2000. since then, various data concerning the epidemiology in snail intermediate hosts and cervid final hosts have been reported. galba truncatula acts as snail intermediate host, and red deer, roe deer and fallow deer act as final hosts. g. truncatula is abundant throughout the region, especially along muddy shores of slow-flowing branches of th ...201424535172
first insight into cd59-like molecules of adult fasciola hepatica.the present study focussed on investigating cd59-like molecules of fasciola hepatica. a cdna encoding a cd59-like protein (termed fhcd59-1) identified previously in the membrane fraction of the f. hepatica tegument was isolated. this homologue was shown to encode a predicted open reading frame (orf) of 122 amino acids (aa) orthologous to human cd59 with a 25 aa signal peptide, a mature protein containing 10 cysteines and a conserved cd59/ly-6 family motif "ccxxxxcn". an analysis of cdnas from tw ...201424955521
fascioloides magna infection causing fatal pulmonary hemorrhage in a steer.a feedlot steer died suddenly without exhibiting signs of clinical illness. cysts containing fascioloides magna were found in the liver and lung. fatal pulmonary hemorrhage was associated with these flukes. neither death nor clinical pulmonary disease has been previously attributed to infections of adult cattle by f. magna.201425392555
development and characterization of multiplex panels of polymorphic microsatellite loci in giant liver fluke fascioloides magna (trematoda: fasciolidae), using next-generation sequencing approach.the microsatellite markers were designed for the giant liver fluke, fascioloides magna, veterinary important liver parasite of free-living and domestic ruminants. due to its geographic distribution (five enzootic regions across usa and canada, three permanent european foci) and invasive character, f. magna is an interesting model for population genetics. out of 667 amplicon candidates generated after ngs, 118 provided the best resolution and were tested with pcr analysis. in total, 56 yielded pc ...201424959976
growth rate of the intermediate snail host galba truncatula influences redial development of the trematode fascioloides magna.a total of 850 pre-adult galba truncatula (shell height, 4 mm), originating from four french snail populations differing in shell height at the adult stage (from 6.5 to 12 mm), were individually subjected at 20°c to single-miracidium infections with fascioloides magna. at day 75 post-exposure, the surviving snails were dissected, and rediae and cercariae were counted. snail groups differed in shell growth during the experiment: from 1.8 ± 0.4 mm in group a up to 4.0 ± 1.1 mm in group d. the prev ...201423710885
molecular identification of fascioloides magna (bassi, 1875) from red deer from south-western poland (lower silesian wilderness) on the basis of internal transcribed spacer 2 (its-2).the study was conducted in 2012-2013 on 75 fecal samples of red deer from the lower silesian wilderness which were examined to determine the prevalence of fascioloides magna in the game population. finding liver fluke eggs in a single sample which were larger in size than fasciola hepatica eggs indicated that further molecular analysis was necessarily. the partial sequence (116 bp long) of its-2 of the investigated eggs was identical to the sequences of f. magna from red deer (cervus elaphus) (g ...201425286665
extension of occurrence area of the american fluke fascioloides magna in south-western poland.liver fluke fascioloides magna is a typical parasite of american cervids. the reason for f. magna to appear in poland territory was bringing the american wapiti deer to those forests around 1850. along with these deer the aforementioned fluke was also introduced. the aim of this study was to present the case of finding of this species in cervids in bory zielonogórskie. samples of deer feces were collected in february 2015 in forest district krzystkowice. a total of 16 samples of feces were exami ...201526342504
an expanding population of the giant liver fluke (fascioloides magna) in elk (cervus canadensis) and other ungulates in canada.giant liver fluke (fascioloides magna) populations readily expand under suitable conditions. although extirpated from the eastern slopes of the canadian rocky mountains in the early 1960s, the fluke reappeared following natural spread through mountain passes from british columbia. herein, we assessed epizootiology of the fluke population two decades later. between 1984 and 1991, 534 ungulates, including 381 elk (cervus canadensis), 68 mule deer (odocoileus hemionus hemionus), 54 white-tailed dee ...201525647594
fascioloides magna--epizootiology in a deer farm in germany.after initial observations of suspicious cases in 2009, the occurrence of fascioloides (f.) magna in deer of a deer farm located in northeastern bavaria, germany, at the border to the czech republic was confirmed in autumn 2011. in march 2012, the deer were treated for fascioloidosis with triclabendazole. to monitor the epizootiology of fascioloidosis in the farm, 80-100 faecal samples were examined for fascioloides eggs at monthly intervals from june 2012 to june 2013 inclusive. in addition, li ...201526054221
multi level ecological fitting: indirect life cycles are not a barrier to host switching and invasion.many invasive species are able to escape from coevolved enemies and thus enjoy a competitive advantage over native species. however, during the invasion phase, non-native species must overcome many ecological and/or physiological hurdles before they become established and spread in their new habitats. this may explain why most introduced species either fail to establish or remain as rare interstitials in their new ranges. studies focusing on invasive species have been based on plants or animals ...201525778909
pharmacological and morphological characteristics of the muscular system of the giant liver fluke (fascioloides magna - bassi 1875).motility is required for feeding, reproduction and maintenance of the fluke in the host's liver. according to that, the neuromuscular system can be an attractive drugable target for chemotherapy. musculature of the fascioloides magna is organized into three layers, an outer circular layer, beneath this layer the longitudinal layer, and third, the oblique, or diagonal layer underlies the longitudinal layer. in our study, the administration of atropine or caffeine did not cause classic muscle cont ...201526429130
landscape influence on spatial patterns of meningeal worm and liver fluke infection in white-tailed deer.parasites that primarily infect white-tailed deer (odocoileus virginianus), such as liver flukes (fascioloides magna) and meningeal worm (parelaphostrongylus tenuis), can cause morbidity and mortality when incidentally infecting moose (alces alces). ecological factors are expected to influence spatial variation in infection risk by affecting the survival of free-living life stages outside the host and the abundance of intermediate gastropod hosts. here, we investigate how ecology influenced the ...201525498206
differences in the compatibility of infection between the liver flukes fascioloides magna and fasciola hepatica in a colombian population of the snail galba sp.experimental infections of galba sp. (origin, colombia) with allopatric isolates of fasciola hepatica from france or fascioloides magna from the czech republic were carried out during five successive snail generations to determine if this lymnaeid might sustain complete larval development of either parasite. in snails exposed to f. hepatica, 7 of 400 snails harboured several rediae and only two snails contained a small number of free cercariae on day 50 post-exposure. in contrast, the intensity ...201525000491
necropsy findings in 62 opportunistically collected free-ranging moose (alces alces) from minnesota, usa (2003-13).the minnesota, us moose population has declined dramatically since the 1990s. all 54 carcasses of moose that died of unknown cause or were euthanized by gun shot by tribal or department of natural resources personnel because of perceived signs of illness between 2003 and 2013 and eight carcasses of moose that died from vehicular accidents between 2009 and 2013 were submitted to the minnesota veterinary diagnostic laboratory and included in our study. the majority of the animals were underweight ...201525390764
in vitro effects of mucus from the mantle of compatible (lymnaea elodes) and incompatible (helisoma trivolvis) snail hosts on fascioloides magna miracidia.the epidermal mucus covering the surface of a snail represents an important barrier to trematode larvae attempting to penetrate the snail and may play a role in mediating snail-trematode compatibility. in this study, facioloides magna miracidia were exposed to mucus harvested from a compatible snail host, lymnaea elodes (palustris), and from an incompatible snail, helisoma trivolvis . in vitro treatment of freshly hatched miracidia with snail-derived mucus exerted dramatically different effects ...201525602486
life history and biology of fascioloides magna (trematoda) and its native and exotic hosts.host-parasite interactions are model systems in a wide range of ecological and evolutionary fields and may be utilized for testing numerous theories and hypotheses in terms of both applied and fundamental research. for instance, they are important in terms of studying coevolutionary arms races, species invasions, and in economic terms the health of livestock and humans. here, i present a comprehensive description of the life history, biogeography, and biology of the giant liver fluke, fascioloid ...201525897378
molecular characterization of fascioloides magna (trematoda: fasciolidae) from south-western poland based on mitochondrial markers.the giant liver fluke, fascioloides magna, is a veterinary important liver parasite of free living and domestic ruminants. this originally north american parasite was introduced along with its cervid hosts to europe where it has established three permanent natural foci - in northern italy, central and southern parts of the czech republic and the danube floodplain forests. the first record on fascioloidosis in poland originated from the lower silesian forest in south-western poland and since then ...201526204195
genetic interrelationships of north american populations of giant liver fluke fascioloides magna.population structure and genetic interrelationships of giant liver fluke fascioloides magna from all enzootic north american regions were revealed in close relation with geographical distribution of its obligate definitive cervid hosts for the first time.201526017023
metabolism of drugs and other xenobiotics in giant liver fluke (fascioloides magna).1. giant liver fluke fascioloides magna is a dangerous parasite, which infects herbivores. it was imported to europe from north america and started to spread. benzimidazoles like albendazole, mebendazole, triclabendazole and salicylanilides closantel and rafoxanide are the most used anthelmintics to control fascioloidosis. however their effect might be altered via drug-metabolizing enzymes of this parasite. 2. the aim of our study was to determine the activities of drug-metabolizing enzymes in f ...201626153440
population structure and dispersal routes of an invasive parasite, fascioloides magna, in north america and europe.fascioloides magna (trematoda: fasciolidae) is an important liver parasite of a wide range of free-living and domestic ruminants; it represents a remarkable species due to its large spatial distribution, invasive character, and potential to colonize new territories. the present study provides patterns of population genetic structure and admixture in f. magna across all enzootic regions in north america and natural foci in europe, and infers migratory routes of the parasite on both continents.201627737705
better alone or in ill company? the effect of migration and inter-species comingling on fascioloides magna infection in elk.migratory movements and alteration of host communities through livestock production are examples of ecological processes that may have consequences on wildlife pathogens. we studied the effect of co-grazing of cattle and wild elk, and of elk migratory behaviour on the occurrence of the giant liver fluke, fascioloides magna, in elk. migratory elk and elk herds with a higher proportion of migratory individuals were significantly less likely to be infected with f. magna. this may indicate a decreas ...201627462721
pulmonary embolization of immature fascioloides magna causing fatal hemothorax confirmed by molecular technique in a heifer in the united states.the current report describes the use of a molecular technique to identify immature fascioloides magna an 18-month-old brangus heifer was found dead in the field without any prior clinical signs. the cause of death was exsanguination into the thoracic cavity associated with pulmonary embolization and infection by immature fascioloides magna resulting in 2 large foci of pulmonary necrosis and focal arteriolar and lung rupture. the liver had a few random migratory tracts with typical iron and porph ...201627423736
complete mitochondrial genome of the giant liver fluke fascioloides magna (digenea: fasciolidae) and its comparison with selected trematodes.representatives of the trematode family fasciolidae are responsible for major socio-economic losses worldwide. fascioloides magna is an important pathogenic liver fluke of wild and domestic ungulates. to date, only a limited number of studies concerning the molecular biology of f. magna exist. therefore, the objective of the present study was to determine the complete mitochondrial (mt) genome sequence of f. magna, and assess the phylogenetic relationships of this fluke with other trematodes bas ...201627492461
a genetic structure of novel population of fascioloides magna from poland, podkarpackie province, indicates an expanding second european natural focus of fascioloidosis.the veterinary important parasite of ruminants, giant liver fluke fascioloides magna(trematoda: fasciolidae), isolated from liver of farmed fallow deer (dama dama) from podkarpackie province (southeastern poland) was genotypized by mitochondrial cytochrome c oxidase (cox1) and nicotinamide dehydrogenase (nad1) markers. the data on this newly emerged population were compared with mitochondrial haplotypes of recently detected polish population of f. magna from lower silesian wilderness (southweste ...201627787209
diagnosis and therapy of liver fluke (fascioloides magna) infection in fallow deer (dama dama) in serbia.giant liver fluke ( fascioloides magna ) infection is an important health problem of cervids in southeastern europe. we measured the prevalence and intensity of infection with f. magna in a fenced area near the danube river in the south bačka district of serbia. parasitologic, pathomorphologic, and histopathologic examinations were conducted from november 2007 to february 2008, beginning with a population of 127 adult fallow deer ( dama dama ). after a positive diagnosis, therapy with triclabend ...201626967130
effect of fascioloides magna (digenea) on fecundity, shell height, and survival rate of pseudosuccinea columella (lymnaeidae).infection with fascioloides magna (digenea) causes serious damage to liver tissue in definitive hosts represented by ruminants, especially cervids. the distribution of f. magna includes the indigenous areas in north america, and the areas to which f. magna was introduced-central europe, southeast europe, and italy. the north american intermediate host of f. magna, the freshwater snail pseudosuccinea columella (lymnaeidae), is an invasive species recorded in south america, the caribbean, africa, ...201627098161
genetic homogeneity of fascioloides magna in austria.the large american liver fluke, fascioloides magna, is an economically relevant parasite of both domestic and wild ungulates. f. magna was repeatedly introduced into europe, for the first time already in the 19th century. in austria, a stable population of f. magna has established in the danube floodplain forests southeast of vienna. the aim of this study was to determine the genetic diversity of f. magna in austria. a total of 26 individuals from various regions within the known area of distrib ...201728807315
fasciolopsis buski (digenea: fasciolidae) from china and india may represent distinct taxa based on mitochondrial and nuclear ribosomal dna sequences.fasciolopsis buski is a zoonotic intestinal fluke infecting humans and pigs, but it has been seriously neglected. it is yet to know whether there is any genetic diversity among f. buski from different geographical locations, particularly in sequences of nuclear ribosomal dna (rdna) and mitochondrial (mt) dna. therefore, we determined the sequences of partial 18s, the complete internal transcribed spacer (its) rdna and the complete mt genome of f. buski from china, compared the rdna and mtdna seq ...201728228149
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