Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
twenty-four years in the mud: what have we learned about the natural history and ecology of the mangrove rivulus, kryptolebias marmoratus?although first described in 1880, kryptolebias marmoratus avoided scientific scrutiny until 1961, when it was identified as the only known selfing hermaphroditic vertebrate. the subsequent intense interest in this fish as a laboratory animal, continuing to this day, might explain the paucity of wild collections, but our collective knowledge now suggests that the inherent difficulty of wild collection is more a matter of "looking in all the wrong places." long thought to be rare in the mangroves, ...201222576816
an insight into the sialome of the frog biting fly, corethrella appendiculata.the nematocera infraorder culicomorpha is believed to have descended from bloodfeeding ancestors over 200 million years ago, generating bloodfeeding and non-bloodfeeding flies in two superfamilies, the culicoidea-containing the mosquitoes, the frog-feeding midges, the chaoboridae, and the dixidae-and the chironomoidea-containing the black flies, the ceratopogonids, the chironomidae, and the thaumaleidae. blood feeding requires many adaptations, including development of a sophisticated salivary p ...201324514880
[seasonal and time dynamics of aedes aegypti (diptera: culicidae) in cienfuegos municipality].dengue is one of the most important vector-borne diseases in las americas due, among other factors, to the increase of aedes aegypti populations.201323431620
secretion of water and ions by malpighian tubules of larval mosquitoes: effects of diuretic factors, second messengers, and salinity.the effects of changes in the salinity of the rearing medium on malpighian tubule fluid secretion and ion transport were examined in larvae of the freshwater mosquito aedes aegypti and the saltwater species ochlerotatus taeniorhynchus. for unstimulated tubules of both species, the k(+) concentration of secreted fluid was significantly lower when larvae were reared in 30% or 100% seawater (o. taeniorhynchus only), relative to tubules from freshwater-reared larvae. the na(+) concentration of secre ...201316691529
vector-borne helminths of dogs and humans in europe.presently, 45% of the total human population of europe, as well as their domestic and companion animals, are exposed to the risk of vector-borne helminths (vbh) causing diseases. a plethora of intrinsic biological and extrinsic factors affect the relationship among helminths, vectors and animal hosts, in a constantly changing environment. although canine dirofilarioses by dirofilaria immitis and dirofilaria repens are key examples of the success of vbh spreading into non-endemic areas, another e ...201323324440
mosquito-host interactions during and after an outbreak of equine viral encephalitis in eastern panama.mosquito blood meals provide information about the feeding habits and host preference of potential arthropod-borne disease vectors. although mosquito-borne diseases are ubiquitous in the neotropics, few studies in this region have assessed patterns of mosquito-host interactions, especially during actual disease outbreaks. based on collections made during and after an outbreak of equine viral encephalitis, we identified the source of 338 blood meals from 10 species of mosquitoes from aruza abajo, ...201324339965
dose-response tests and semi-field evaluation of lethal and sub-lethal effects of slow release pyriproxyfen granules (sumilarv®0.5g) for the control of the malaria vectors anopheles gambiae sensu lato.recently research has shown that larviciding can be an effective tool for integrated malaria vector control. nevertheless, the uptake of this intervention has been hampered by the need to re-apply larvicides frequently. there is a need to explore persistent, environmentally friendly larvicides for malaria vector control to reduce intervention efforts and costs by reducing the frequency of application. in this study, the efficacy of a 0.5% pyriproxyfen granule (surmilarv®0.5g, sumitomo chemicals) ...201323497149
nature, nurture and evolution of intra-species variation in mosquito arbovirus transmission competence.mosquitoes vary in their competence or ability to transmit arthropod-borne viruses (arboviruses). many arboviruses cause disease in humans and animals. identifying the environmental and genetic causes of variation in mosquito competence for arboviruses is one of the great challenges in public health. progress identifying genetic (nature) and environmental (nurture) factors influencing mosquito competence for arboviruses is reviewed. there is great complexity in the various traits that comprise m ...201323343982
rna viruses can hijack vertebrate micrornas to suppress innate immunity.currently, there is little evidence for a notable role of the vertebrate microrna (mirna) system in the pathogenesis of rna viruses. this is primarily attributed to the ease with which these viruses mutate to disrupt recognition and growth suppression by host mirnas. here we report that the haematopoietic-cell-specific mirna mir-142-3p potently restricts the replication of the mosquito-borne north american eastern equine encephalitis virus in myeloid-lineage cells by binding to sites in the 3' n ...201324352241
freshwater biodiversity and aquatic insect diversification.inland waters cover less than 1% of earth's surface but harbor more than 6% of all insect species: nearly 100,000 species from 12 orders spend one or more life stages in freshwater. little is known about how this remarkable diversity arose, although allopatric speciation and ecological adaptation are thought to be primary mechanisms. freshwater habitats are highly susceptible to environmental change and exhibit marked ecological gradients. standing waters appear to harbor more dispersive species ...201324160433
the risk of rift valley fever virus introduction and establishment in the united states and european union.rift valley fever virus (rvfv) is an arthropod-borne disease resulting in severe morbidity and mortality in both human and ruminant populations. first identified in kenya in 1930, the geographical range of rvfv has been largely constrained to the african continent, yet has recently spread to new regions, and is identified as a priority disease with potential for geographic emergence. we present a systematic literature review assessing the potential for rvfv introduction and establishment in the ...201326038446
zoonotic encephalitides caused by arboviruses: transmission and epidemiology of alphaviruses and flaviviruses.in this review, we mainly focus on zoonotic encephalitides caused by arthropod-borne viruses (arboviruses) of the families flaviviridae (genus flavivirus) and togaviridae (genus alphavirus) that are important in both humans and domestic animals. specifically, we will focus on alphaviruses (eastern equine encephalitis virus, western equine encephalitis virus, venezuelan equine encephalitis virus) and flaviviruses (japanese encephalitis virus and west nile virus). most of these viruses were origin ...201324427764
ires-based venezuelan equine encephalitis vaccine candidate elicits protective immunity in mice.venezuelan equine encephalitis virus (veev) is an arbovirus that causes periodic outbreaks that impact equine and human populations in the americas. one of the veev subtypes located in mexico and central america (ie) has recently been recognized as an important cause of equine disease and death, and human exposure also appears to be widespread. here, we describe the use of an internal ribosome entry site (ires) from encephalomyocarditis virus to stably attenuate veev, creating a vaccine candidat ...201323351391
transmission of pathogens by stomoxys flies (diptera, muscidae): a review.stomoxys flies are mechanical vectors of pathogens present in the blood and skin of their animal hosts, especially livestock, but occasionally humans. in livestock, their direct effects are disturbance, skin lesions, reduction of food intake, stress, blood loss, and a global immunosuppressive effect. they also induce the gathering of animals for mutual protection; meanwhile they favor development of pathogens in the hosts and their transmission. their indirect effect is the mechanical transmissi ...201323985165
what is the risk for exposure to vector-borne pathogens in united states national parks?united states national parks attract > 275 million visitors annually and collectively present risk of exposure for staff and visitors to a wide range of arthropod vector species (most notably fleas, mosquitoes, and ticks) and their associated bacterial, protozoan, or viral pathogens. we assessed the current state of knowledge for risk of exposure to vector-borne pathogens in national parks through a review of relevant literature, including internal national park service documents and organismal ...201323540107
pathogenic landscape of transboundary zoonotic diseases in the mexico-us border along the rio grande.transboundary zoonotic diseases, several of which are vector borne, can maintain a dynamic focus and have pathogens circulating in geographic regions encircling multiple geopolitical boundaries. global change is intensifying transboundary problems, including the spatial variation of the risk and incidence of zoonotic diseases. the complexity of these challenges can be greater in areas where rivers delineate international boundaries and encompass transitions between ecozones. the rio grande serve ...201425453027
effects of photoperiod on the induction of embryonic diapause in aedes taeniorhynchus (diptera: culicidae). 20144045935
biological differences between brackish and fresh water-derived aedes aegypti from two locations in the jaffna peninsula of sri lanka and the implications for arboviral disease transmission.the mainly fresh water arboviral vector aedes aegypti l. (diptera: culicidae) can also undergo pre-imaginal development in brackish water of up to 15 ppt (parts per thousand) salt in coastal areas. we investigated differences in salinity tolerance, egg laying preference, egg hatching and larval development times and resistance to common insecticides in ae. aegypti collected from brackish and fresh water habitats in jaffna, sri lanka. brackish water-derived ae. aegypti were more tolerant of salin ...201425170879
role of micrornas in arbovirus/vector interactions.the role of micrornas (mirnas) as small non-coding rnas in regulation of gene expression has been recognized. they appear to be involved in regulation of a wide range of cellular pathways that affect several biological processes such as development, the immune system, survival, metabolism and host-pathogen interactions. arthropod-borne viruses impose great economic and health risks around the world. recent advances in mirna biology have shed some light on the role of these small rnas in vector-v ...201425251636
modeling dynamics of culex pipiens complex populations and assessing abatement strategies for west nile virus.the primary mosquito species associated with underground stormwater systems in the united states are the culex pipiens complex species. this group represents important vectors of west nile virus (wnv) throughout regions of the continental u.s. in this study, we designed a mathematical model and compared it with surveillance data for the cx. pipiens complex collected in beaufort county, south carolina. based on the best fit of the model to the data, we estimated parameters associated with the eff ...201425268229
dogs, cats, parasites, and humans in brazil: opening the black box.dogs and cats in brazil serve as primary hosts for a considerable number of parasites, which may affect their health and wellbeing. these may include endoparasites (e.g., protozoa, cestodes, trematodes, and nematodes) and ectoparasites (i.e., fleas, lice, mites, and ticks). while some dog and cat parasites are highly host-specific (e.g., aelurostrongylus abstrusus and felicola subrostratus for cats, and angiostrongylus vasorum and trichodectes canis for dogs), others may easily switch to other h ...201424423244
comparing aedes vigilax eggshell densities in saltmarsh and mangrove systems with implications for management.aedes vigilax (skuse), a nuisance and disease vector, is prolific in intertidal wetlands in australia. aedine mosquitoes oviposit directly onto substrate. the eggshells are relatively stable spatially and temporally, providing an estimate of mosquito larval production. the aims of the research were to compare, at a general level, oviposition in mangroves and saltmarshes, and to compare oviposition between different habitats within mangroves and saltmarshes. the results indicated that there were ...201426462954
analysis of the genetic variability and structure of ochlerotatus taeniorhynchus (diptera: culicidae) populations from the colombian atlantic coast on the basis of random amplified polymorphic dna markers.ochlerotatus taeniorhynchus (diptera: culicidae) is a mosquito, which is an efficient vector of the virus causing epidemic-epizootic venezuelan equine encephalitis in colombia. this study used 9 random amplified polymorphic dna (rapd) markers to analyze the mosquito's genetic variability and genetic structure of 122 specimens in 7 populations from the colombian atlantic coast. assuming that all loci were in hardy-weinberg equilibrium, diversity statistics and analyses were performed. the average ...201424938703
predicting the mosquito species and vertebrate species involved in the theoretical transmission of rift valley fever virus in the united states.rift valley fever virus (rvfv) is a mosquito-borne virus in the family bunyaviridiae that has spread throughout continental africa to madagascar and the arabian peninsula. the establishment of rvfv in north america would have serious consequences for human and animal health in addition to a significant economic impact on the livestock industry. published and unpublished data on rvfv vector competence, vertebrate host competence, and mosquito feeding patterns from the united states were combined ...201425211133
insect-specific viruses detected in laboratory mosquito colonies and their potential implications for experiments evaluating arbovirus vector competence.recently, there has been a dramatic increase in the detection and characterization of insect-specific viruses in field-collected mosquitoes. evidence suggests that these viruses are ubiquitous in nature and that many are maintained by vertical transmission in mosquito populations. some studies suggest that the presence of insect-specific viruses may inhibit replication of a super-infecting arbovirus, thus altering vector competence of the mosquito host. accordingly, we screened our laboratory mo ...201425510714
diversity of mosquitoes and the aquatic insects associated with their oviposition sites along the pacific coast of mexico.the abundance, richness and diversity of mosquitoes and aquatic insects associated with their oviposition sites were surveyed along eight states of the pacific coast of mexico. diversity was estimated using the shannon index (h'), similarity measures and cluster analysis.201424450800
male aedes aegypti mosquitoes use jh iii transferred during copulation to influence previtellogenic ovary physiology and affect the reproductive output of female mosquitoes.the effect of male accessory gland substances on female reproductive physiology has been previously described as "activating" egg development. however, no mechanism has been described that can explain how male mosquitoes are able to influence egg development in female mosquitoes. to investigate how male mosquitoes are able to influence ovarian physiology and reproductive output we explored three main questions: (1) do mating and male accessory gland substances affect ovarian physiology and alter ...201424657670
evans blue as a simple method to discriminate mosquitoes' feeding choice on small laboratory animals.temperature, humidity, vision, and particularly odor, are external cues that play essential roles to mosquito blood feeding and oviposition. entomological and behavioral studies employ well-established methods to evaluate mosquito attraction or repellency and to identify the source of the blood meal. despite the efficacy of such methods, the costs involved in the production or acquisition of all parts, components and the chemical reagents involved are unaffordable for most researchers from poor ...201425333369
effects of neonicotinoids and fipronil on non-target invertebrates.we assessed the state of knowledge regarding the effects of large-scale pollution with neonicotinoid insecticides and fipronil on non-target invertebrate species of terrestrial, freshwater and marine environments. a large section of the assessment is dedicated to the state of knowledge on sublethal effects on honeybees (apis mellifera) because this important pollinator is the most studied non-target invertebrate species. lepidoptera (butterflies and moths), lumbricidae (earthworms), apoidae sens ...201425223353
neurophysiological and behavioral responses of gypsy moth larvae to insect repellents: deet, ir3535, and picaridin.the interactions between insect repellents and the olfactory system have been widely studied, however relatively little is known about the effects of repellents on the gustatory system of insects. in this study, we show that the gustatory receptor neuron (grn) located in the medial styloconic sensilla on the maxillary palps of gypsy moth larvae, and known to be sensitive to feeding deterrents, also responds to the insect repellents deet, ir3535, and picaridin. these repellents did not elicit res ...201424955823
interaction of light regimes and circadian clocks modulate timing of pre-adult developmental events in drosophila.circadian clocks have been postulated to regulate development time in several species of insects including fruit flies drosophila melanogaster. previously we have reported that selection for faster pre-adult development reduces development time (by ~19 h or ~11%) and clock period (by ~0.5 h), suggesting a role of circadian clocks in the regulation of development time in d. melanogaster. we reasoned that these faster developing flies could serve as a model to study stage-specific interaction of c ...201424885932
a mosquito survey of the twin-island caribbean nation of saint kitts and nevis, 2010.adult mosquito surveys of saint kitts and nevis (skn) were performed in the dry season (march 16-23, 2010) in saint kitts, and the rainy season (october 18-25, 2010) in skn. biogents (bg) sentinel traps were set with co₂and bg lure in urban, rural, mangrove, and dry forest habitats. mosquitoes were identified to species, and reverse transcription-polymerase chain reaction was performed on potential vector species for dengue virus (denv), chikungunya virus (chikv), and west nile virus (wnv). the ...201526675458
in-depth study of mollivirus sibericum, a new 30,000-y-old giant virus infecting acanthamoeba.acanthamoeba species are infected by the largest known dna viruses. these include icosahedral mimiviruses, amphora-shaped pandoraviruses, and pithovirus sibericum, the latter one isolated from 30,000-y-old permafrost. mollivirus sibericum, a fourth type of giant virus, was isolated from the same permafrost sample. its approximately spherical virion (0.6-µm diameter) encloses a 651-kb gc-rich genome encoding 523 proteins of which 64% are orfans; 16% have their closest homolog in pandoraviruses an ...201526351664
evaluation of the efficacy, potential for vector transmission, and duration of immunity of mp-12, an attenuated rift valley fever virus vaccine candidate, in sheep.rift valley fever virus (rvfv) causes serious disease in ruminants and humans in africa. in north america, there are susceptible ruminant hosts and competent mosquito vectors, yet there are no fully licensed animal vaccines for this arthropod-borne virus, should it be introduced. studies in sheep and cattle have found the attenuated strain of rvfv, mp-12, to be both safe and efficacious based on early testing, and a 2-year conditional license for use in u.s. livestock has been issued. the purpos ...201526041042
risk factors associated with rift valley fever epidemics in south africa in 2008-11.rift valley fever (rvf) is a zoonotic and vector-borne disease, mainly present in africa, which represents a threat to human health, animal health and production. south africa has experienced three major rvf epidemics (1950-51, 1973-75 and 2008-11). due to data scarcity, no previous study has quantified risk factors associated with rvf epidemics in animals in south africa. using the 2008-11 epidemic datasets, a retrospective longitudinal study was conducted to identify and quantify spatial and t ...201525804974
molecular identification of saint louis encephalitis virus genotype iv in colombia.saint louis encephalitis virus (slev) is a member of the japanese-encephalitis virus serocomplex of the genus flavivirus. slev is broadly distributed in the americas and the caribbean islands, where it is usually transmitted by mosquitoes of the genus culex and primarily to birds and mammalian-hosts. humans are occasionally infected by the virus and are dead-end hosts. slev causes encephalitis in temperate regions, while in tropical regions of the americas, several human cases and a wide biologi ...201526313538
allethrin-based mosquito control device causing knockdown, morbidity, and mortality in four species of field-caught mosquitoes (diptera: culicidae).a mosquito control device marketed for spatial repellency, the thermacell mosquito repellent appliance, was evaluated in semifield trials against multiple field-caught species of mosquito. using paper and mesh cages, mosquito test groups of at least 30 mosquitoes were suspended in a 2,337 cubic foot outdoor space while two thermacell repellent devices were active. after 30 min of treatment, cages were moved to the laboratory to observe knockdown, morbidity, and mortality for 24 h. species tested ...201526335485
[do some conditions contribute to the reemergence of the venezuelan equine encephalitis virus in the colombian alta guajira?].in the last 18 years, epizootics of venezuelan equine encephalitis have not occurred in places with historic epidemic register (1925-1995) in the guajira peninsula, colombia.201526148035
systematic revision of the adeleid haemogregarines, with creation of bartazoon n. g., reassignment of hepatozoon argantis garnham, 1954 to hemolivia, and molecular data on hemolivia stellata.life cycles and molecular data for terrestrial haemogregarines are reviewed in this article. collection material was re-examined: hepatozoon argantis garnham, 1954 in argas brumpti was reassigned to hemolivia as hemolivia argantis (garnham, 1954) n. comb.; parasite dna was extracted from a tick crush on smear of an archived slide of hemolivia stellata in amblyomma rotondatum, then the 18s ssrrna gene was amplified by pcr. a systematic revision of the group is proposed, based on biological life c ...201526551414
vector-host interactions of culiseta melanura in a focus of eastern equine encephalitis virus activity in southeastern virginia.eastern equine encephalitis virus (eeev) causes a highly pathogenic mosquito-borne zoonosis that is responsible for sporadic outbreaks of severe illness in humans and equines in the eastern usa. culiseta (cs.) melanura is the primary vector of eeev in most geographic regions but its feeding patterns on specific avian and mammalian hosts are largely unknown in the mid-atlantic region. the objectives of our study were to: 1) identify avian hosts of cs. melanura and evaluate their potential role in ...201526327226
temperature-sensitive mutations for live-attenuated rift valley fever vaccines: implications from other rna viruses.rift valley fever (rvf) is a mosquito-borne zoonotic disease endemic to the african continent. rvf is characterized by high rate of abortions in ruminants and hemorrhagic fever, encephalitis, or blindness in humans. rvf is caused by the rift valley fever virus (rvfv: genus phlebovirus, family bunyaviridae). vaccination is the only known effective strategy to prevent the disease, but there are no licensed rvf vaccines available for humans. a live-attenuated vaccine candidate derived from the wild ...201526322023
the 5' and 3' ends of alphavirus rnas--non-coding is not non-functional.the non-coding regions found at the 5' and 3' ends of alphavirus genomes regulate viral gene expression, replication, translation and virus-host interactions, which have significant implications for viral evolution, host range, and pathogenesis. the functions of these non-coding regions are mediated by a combination of linear sequence and structural elements. the capped 5' untranslated region (utr) contains promoter elements, translational regulatory sequences that modulate dependence on cellula ...201525630058
tissue barriers to arbovirus infection in mosquitoes.arthropod-borne viruses (arboviruses) circulate in nature between arthropod vectors and vertebrate hosts. arboviruses often cause devastating diseases in vertebrate hosts, but they typically do not cause significant pathology in their arthropod vectors. following oral acquisition of a viremic bloodmeal from a vertebrate host, the arbovirus disease cycle requires replication in the cellular environment of the arthropod vector. once the vector has become systemically and persistently infected, the ...201526184281
wolbachia biocontrol strategies for arboviral diseases and the potential influence of resident wolbachia strains in mosquitoes.arboviruses transmitted by mosquitoes are a major cause of human disease worldwide. the absence of vaccines and effective vector control strategies has resulted in the need for novel mosquito control strategies. the endosymbiotic bacterium wolbachia has been proposed to form the basis for an effective mosquito biocontrol strategy. resident strains of wolbachia inhibit viral replication in drosophila fruit flies and induce a reproductive phenotype known as cytoplasmic incompatibility that allows ...201626925368
molecular detection of flaviviruses and alphaviruses in mosquitoes (diptera: culicidae) from coastal ecosystems in the colombian caribbean.arboviruses belonging to the genera flavivirus and alphavirus were detected in mosquitoes in a rural area of san bernardo del viento (córdoba, colombia). a total of 22,180 mosquitoes were collected, sorted into 2,102 pools, and tested by generic/nested reverse transcription-polymerase chain reaction. venezuelan equine encephalitis virus, dengue virus, west nile virus, st. louis encephalitis virus, yellow fever virus, and culex flavivirus were detected and identified by sequencing. the detection ...201627706377
countrywide serological evaluation of canine prevalence for anaplasma spp., borrelia burgdorferi (sensu lato), dirofilaria immitis and ehrlichia canis in mexico.canine vector-borne diseases (cvbd) have become a major concern for canine and human public health. the aim of the study described here is to add epidemiological data regarding four pathogens responsible for cvbd, namely anaplasmosis, borreliosis, dirofilariosis and ehrlichiosis in a national survey conducted in mexico.201627474020
the epidemiology of rift valley fever in mayotte: insights and perspectives from 11 years of data.rift valley fever (rvf) is a zoonotic arboviral disease that is a threat to human health, animal health and production, mainly in sub-saharan africa. rvf virus dynamics have been poorly studied due to data scarcity. on the island of mayotte in the indian ocean, off the southeastern african coast, rvf has been present since at least 2004. several retrospective and prospective serological surveys in livestock have been conducted over eleven years (2004-15). these data are collated and presented he ...201627331402
vertical transmission of zika virus in aedes aegypti mosquitoes.previous experimental studies have demonstrated that a number of mosquito-borne flavivirus pathogens are vertically transmitted in their insect vectors, providing a mechanism for these arboviruses to persist during adverse climatic conditions or in the absence of a susceptible vertebrate host. in this study, designed to test whether zika virus (zikv) could be vertically transmitted, female aedes aegypti and aedes albopictus were injected with zikv, and their f1 adult progeny were tested for zikv ...201627573623
research contributing to improvements in controlling florida's mosquitoes and mosquito-borne diseases.research on mosquitoes and mosquito-borne diseases has contributed to improvements in providing effective, efficient, and environmentally proper mosquito control. florida has benefitted from several research accomplishments that have increased the state's mosquito control capabilities. research with florida's mosquitoes has resulted in the development of ecologically sound management of mosquito impoundments on florida's east coast. this strategy, called rotational impoundment management (rim), ...201627690112
has rift valley fever virus evolved with increasing severity in human populations in east africa?rift valley fever (rvf) outbreaks have occurred across eastern africa from 1912 to 2010 approximately every 4-15 years, most of which have not been accompanied by significant epidemics in human populations. however, human epidemics during rvf outbreaks in eastern africa have involved 478 deaths in 1998, 1107 reported cases with 350 deaths from 2006 to 2007 and 1174 cases with 241 deaths in 2008. we review the history of rvf outbreaks in eastern africa to identify the epidemiological factors that ...201627329846
mosquito oviposition behavior and vector control.the burden of gene transfer from one mosquito generation to the next falls on the female and her eggs. the selection of an oviposition site that guarantees egg and larval survival is a critical step in the reproductive process. the dangers associated with ephemeral aquatic habitats, lengthy droughts, freezing winters, and the absence of larval nutrition makes careful oviposition site selection by a female mosquito extremely important. mosquito species exhibit a remarkable diversity of ovipositio ...201627869724
non-human primate antibody response to mosquito salivary proteins: implications for dengue virus transmission in puerto rico.an important step to incriminate a mosquito as a vector of a disease pathogen is finding evidence of direct contact between the mosquito and humans. typically, this is accomplished through landing/biting catches, or host blood meal analysis in engorged mosquitoes via immunologic assays. an alternate approach is to identify the presence of specific mosquito anti-saliva protein antibodies in the blood of exposed hosts. following the discovery of dengue infected, free roaming non-human primates in ...201627593498
zika virus vector competency of mosquitoes, gulf coast, united states.zika virus has recently spread throughout the americas. although aedes aegypti mosquitoes are considered the primary vector, culex quinquefasciatus and mosquitoes of other species may also be vectors. we tested cx. quinquefasciatus and ae. taeniorhynchus mosquitoes from the us gulf coast; both were refractory to infection and incapable of transmission.201728005002
the distribution of mosquitoes across an altitudinal gradient in the galapagos islands.an avian malaria parasite (genus plasmodium) has been detected consistently in the galapagos penguin (spheniscus mendiculus) and less frequently in some passerines. we sampled three resident mosquito species (aedes taeniorhynchus, culex quinquefasciatus, and aedes aegypti) using cdc light and gravid traps on three islands in 2012, 2013, and 2014. we sampled along altitudinal gradients to ask whether there are mosquito-free refugia at higher elevations as there are in hawaii. we captured both ae. ...201729125252
effects of mosquito control adulticides on sterile cochliomyia hominivorax (diptera: calliphoridae).effects of mosquito control adulticides on sterile screwworm flies, cochliomyia hominivorax (coquerel) (diptera: calliphoridae), were investigated via bottle bioassays, outdoor cage tests, and exposure to treated vegetation. in bottle bioassays, 43 μg of permethrin via dilution of evoluer, 474.56 μg of malathion via dilution of fyfanon, and 25 μg of naled via dilution of dibrom concentrate were used to challenge screwworm flies. permethrin was more toxic to screwworm flies than was malathion, wh ...201829365134
screening of natural wolbachia infection in aedes aegypti, aedes taeniorhynchus and culex quinquefasciatus from guadeloupe (french west indies).guadeloupe islands are threatened by several mosquito-borne viruses such as dengue, chikungunya, zika and west nile virus. it appears essential to look for alternative mosquito control methods such as the incompatible insect technique (itt) aiming at sterilizing wild females by inundative releases of incompatible males. before considering the implementation of such a strategy, the characterization of genetic diversity of the endocellular bacterium wolbachia regarding the local mosquito populatio ...201829908171
detection of novel and recognized rna viruses in mosquitoes from the yucatan peninsula of mexico using metagenomics and characterization of their in vitro host ranges.a metagenomics approach was used to detect novel and recognized rna viruses in mosquitoes from the yucatan peninsula of mexico. a total of 1359 mosquitoes of 7 species and 5 genera (aedes, anopheles, culex, mansonia and psorophora) were sorted into 37 pools, homogenized and inoculated onto monolayers of aedes albopictus (c6/36) cells. a second blind passage was performed and then total rna was extracted and analysed by rna-seq. two novel viruses, designated uxmal virus and mayapan virus, were id ...201830412047
microsatellite markers transferability in culex nigripalpus (diptera: culicidae).microsatellite loci specifically designed and optimized for secondary vectors and neglected species of mosquitoes are lacking. cross-species transferability of microsatellite loci between close taxa has been used as a reliable option, being successfully done for aedes taeniorhynchus wiedemann (diptera: culicidae), triatoma sordida stål (hemiptera: reduviidae), and aedes fluviatilis lutz (diptera: culicidae). nonetheless, prior to the utilization of transferred microsatellites, they must be asses ...201829767738
dataset for mosquito collections on big pine key, florida, usa.the florida keys mosquito control district has deployed dry ice-baited light traps to monitor mosquito (diptera: culicidae) populations throughout the florida keys starting in 1998. american biophysics company traps were deployed throughout the year at the same collection point. traps were placed in the late afternoon and collected the following morning. common mosquitoes are the black salt marsh mosquito, aedes taeniorhynchus (wiedemann), the crabhole mosquito, deinocerites cancer theobald, the ...201931667279
assessing the blood meal hosts of culex quinquefasciatus and aedes taeniorhynchus in isla santa cruz, galápagos.blood meal host selection by mosquito vectors is an important component in understanding disease dynamics of pathogens that threaten endemic fauna in isolated islands such as galápagos. research on the feeding behavior of mosquitoes can provide clues to the hosts and vectors involved in disease transmission. this information is particularly critical for endemic wildlife fauna in island systems that have evolved without resistance to novel diseases such as avian malaria. the aims of this study we ...201931842984
mosquito abundance in a dirofilaria immitis hotspot in the eastern state of rio de janeiro, brazil.coastal lowlands in the state of rio de janeiro, brazil, support high numbers of heartworm-infected dogs. microfilariae of heartworm need to be ingested by a potencial mosquito vector in order to develop into infective larvae and infect a new host. ochlerotatus taeniorhynchus and ochlerotatus scapularis are the primary vector species in the coastal lowlands of metropolitan rio de janeiro; thus, the aim of this study was to investigate whether these two species were abundant enough at the heartwo ...201931796177
the impact of hurricane irma on population density of the black salt-marsh mosquito, aedes taeniorhynchus, in collier county, florida.in 2017, the southeastern usa and caribbean endured an extremely active year for tropical storm and hurricane activity. on september 10, 2017, hurricane irma made a 2nd landfall in florida, striking collier county-including naples and marco island, fl. areas affected by hurricane irma are abundant in salt-marsh and mangrove swamps, prime habitats for the black salt-marsh mosquito, aedes taeniorhynchus. using both human landing rate and biogents bg-counter trap data, here we report the year-to-ye ...201931442180
haemagogus leucocelaenus and haemagogus janthinomys are the primary vectors in the major yellow fever outbreak in brazil, 2016-2018.the yellow fever virus (yfv) caused a severe outbreak in brazil in 2016-2018 that rapidly spread across the atlantic forest in its most populated region without viral circulation for almost 80 years. a comprehensive entomological survey combining analysis of distribution, abundance and yfv natural infection in mosquitoes captured before and during the outbreak was conducted in 44 municipalities of five brazilian states. in total, 17,662 mosquitoes of 89 species were collected. before evidence of ...201930866775
entomo-virological surveillance strategy for dengue, zika and chikungunya arboviruses in field-caught aedes mosquitoes in an endemic urban area of the northeast of brazil.aedes spp. are considered the main vectors of dengue (denv), zika (zikv) and chikungunya (chikv) viruses in the world. arbovirus detection in aedes mosquitoes can alert authorities to possible outbreaks, reducing the impact of these diseases. the purpose of this study was to perform an operational strategy for virological surveillance of denv, zikv and chikv in adult aedes aegypti and aedes albopictus mosquitoes captured at different key-sites in an endemic urban area of the northeast region of ...201931194961
zika virus detection, isolation and genome sequencing through culicidae sampling during the epidemic in vitória, espírito santo, brazil.zika virus (zikv) has been isolated from many mosquito species in nature, but it is believed that the main vectors in urban environments are species of the genus aedes. here, we detected and isolated zikv in samples from aedes aegypti, aedes taeniorhynchus and culex quinquefasciatus, collected during the zika epidemic in vitória, southeast brazil. using quantitative real-time polymerase chain reaction, zikv detection was performed in mosquito samples collected from february to april 2016.201931068218
Refresh
firstprev
1234
nextlast
Displaying items 301 - 365 of 365