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high prevalence of fluoroquinolone- and methicillin-resistant staphylococcus pseudintermedius isolates from canine pyoderma and otitis externa in veterinary teaching hospital.recently, a total of 74 staphylococcus pseudintermedius isolates were collected from clinical cases of canine pyoderma and otitis externa in korea. in this study, we examined in vitro fluoroquinolone resistance among those isolates using a standard disk diffusion technique. the results demonstrated that approximately 18.9% to 27.0% of the isolates possessed bacterial resistance to both veterinary- and human-licensed fluoroquinolones except one isolate, including moxifloxacin (18.9% resistance), ...201020467256
in vitro antimicrobial efficacy of β-defensin 3 against staphylococcus pseudintermedius isolates from healthy and atopic canine skin.β-defensins (bds) are highly conserved antimicrobial peptides important in innate defence against bacteria. β-defensin 3 has a specific role in protecting the skin. this study quantified the minimal inhibitory concentration (mic) of human (h)bd3 against staphylococcus pseudintermedius isolates from atopic and healthy dogs. single colony isolates (1 × 10(5) colony-forming units/ml log phase) were cultured with doubling dilutions of hbd3 in sodium phosphate buffer from 0.8 to 50 μg/ml at 37 °c for ...201020492622
prevalence of genes for enterotoxins, toxic shock syndrome toxin 1 and exfoliative toxin among clinical isolates of staphylococcus pseudintermedius from canine origin.a total of 74 staphylococcus pseudintermedius strains were isolated from the 99 clinical cases of canine pyoderma or chronic otitis in our veterinary teaching hospital during may 2006-february 2008. in this study, we examined the genetic distribution of staphylococcal pyogenic toxins such as staphylococcal enterotoxins a (sea), b (seb), c (sec), d (sed), e (see), and toxic shock syndrome toxin 1 (tst) as well as the previously characterized s. intermedius exfoliative toxin (siet) among those iso ...201020500497
molecular analysis of methicillin-resistant staphylococcus pseudintermedius of feline origin from different european countries and north america. 201020534627
antimicrobial susceptibility and methicillin resistance in staphylococcus pseudintermedius and staphylococcus schleiferi subsp. coagulans isolated from dogs with pyoderma in japan.to understand species distribution, trends of antimicrobial susceptibility and prevalence of methicillin resistance in canine staphylococci in japan, 190 coagulase-positive staphylococci (cops) were isolated from dogs with pyoderma in 2 japanese veterinary referral hospitals. using a multiplex polymerase chain reaction (m-pcr) method, two cops species were identified: 170 staphylococcus pseudintermedius (89.5%) and 20 s. schleiferi subsp. coagulans isolates (10.5%). in these isolates, susceptibi ...201020703027
methicillin-resistant staphylococcus pseudintermedius among dogs admitted to a small animal hospital.the aim of this study was to determine the frequency of carriage of methicillin-resistant staphylococcus pseudintermedius (mrsp) among dogs admitted to a small animal hospital during a 17-month period, to characterize these isolates and to initially screen for possible factors associated with mrsp carriage. swabs were taken from the nose/pharynx and the perineum as well as from wounds and skin infections (if present) of 814 dogs before entering the small animal hospital. a questionnaire for back ...201021247709
occurrence and molecular characteristics of methicillin-resistant staphylococcus aureus and methicillin-resistant staphylococcus pseudintermedius in an academic veterinary hospital.recently, methicillin-resistant staphylococcus aureus (mrsa) and methicillin-resistant staphylococcus pseudintermedius (mrsp) have been increasingly isolated from veterinarians and companion animals. with a view to preventing the spread of mrsa and mrsp, we evaluated the occurrence and molecular characteristics of each in a veterinary college. mrsa and mrsp were isolated from nasal samples from veterinarians, staff members, and veterinary students affiliated with a veterinary hospital. using ste ...201020543040
human infection associated with methicillin-resistant staphylococcus pseudintermedius st71. 201020601356
clonal spread of methicillin-resistant staphylococcus pseudintermedius in europe and north america: an international multicentre study.the aim of this study was to determine the phenotypic and genotypic resistance profiles of methicillin-resistant staphylococcus pseudintermedius (mrsp) and to examine the clonal distribution in europe and north america.201020348087
further diversity of the staphylococcus intermedius group and heterogeneity in the mboi restriction site used for staphylococcus pseudintermedius species identification.species identification of 200 beta-hemolysin-producing canine staphylococcal isolates was performed using a recently described polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) method (based on mboi restriction of a pta gene fragment), supplemented with biochemical testing and sequencing of housekeeping genes. the pcr-rflp method misclassified a small fraction (approximately 1%) of the staphylococcus pseudintermedius population as a result of heterogeneity in the mboi ...201020807936
reclassification of the listeria-camp test strain atcc 49444 staphylococcus aureus as staphylococcus pseudintermedius.atcc 49444, originally designated as staphylococcus aureus, has been cited as a component strain in the camp test for identification of listeria monocytogenes. a polyphasic study, in which molecular data were combined with cytochemical properties and physiological characteristics, identified this isolate as staphylococcus pseudintermedius. the nucleotide sequences of the 16s rrna and soda genes from atcc 49444 were determined and found to be identical to those of other s. pseudintermedius strain ...201020819366
identification of a novel staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs.staphylococcal exfoliative toxins are involved in some cutaneous infections in mammals by targeting desmoglein 1 (dsg1), a desmosomal cell-cell adhesion molecule. recently, an exfoliative toxin gene (exi) was identified in staphylococcus pseudintermedius isolated from canine pyoderma. the aim of this study was to identify novel exfoliative toxin genes in s. pseudintermedius. here, we describe a novel orf in the genome of s. pseudintermedius isolated from canine impetigo, whose deduced amino acid ...201020875053
in vitro antimicrobial resistance of staphylococci isolated from canine urinary tract infection.this study determined the diversity of species and antimicrobial resistance of staphylococci isolated from dogs with a presumptive diagnosis of urinary tract infection (uti). urine samples from 348 dogs with clinical signs of uti, according to clinical examination and urinalysis, were processed for isolation of staphylococcus. colonies in pure culture were identified by biochemical reactions and tested for susceptibility to 15 antimicrobials. seventy isolates of staphylococci were obtained (20.1 ...201020885826
isolation of methicillin-resistant staphylococcus pseudintermedius from breeding dogs.the overuse of antimicrobials can select resistant bacteria strains; staphylococci have the ability to become resistant to all beta-lactam antimicrobials and are a significant concern in human medicine and a growing issue for veterinary medicine. because antimicrobials are sometimes incorrectly used in breeding kennels, the objective of the work was to assess the occurrence of methicillin-resistant coagulase-positive staphylococci in breeding dogs. the research was carried out in 13 kennels that ...201020961604
multidrug- and methicillin resistant staphylococcus pseudintermedius as a cause of canine pyoderma: a case report.a case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant staphylococcus pseudintermedius (mrsp) is described. after initial diagnostics of mrsp, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. mrsp was constantly isolated from the skin and once from the nose. since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet's therapy. thereby, the mal ...201021038805
characterization of a new sccmec element in staphylococcus cohnii.many sccmec elements of coagulase-negative staphylococci (cons) could not be typed using multiplex pcr. such a 'non-typable' sccmec was encountered in a staphylococcus cohnii isolate.201021103346
retrospective study of necropsy-associated coagulase-positive staphylococci in horses.coagulase-positive staphylococci (cops) are potential causative agents of equine infections, but they are rarely responsible for the death of the animal. in the current study, staphylococci implicated in the death or euthanasia of horses were retrospectively studied in 3,457 necropsies performed over a decade (1995-2006). morbidity associated with cops was 1.7%, representing 60 isolates of cops, which were identified as staphylococcus aureus (59) and staphylococcus pseudintermedius (1). coagulas ...201021088182
escherichia coli and selected veterinary and zoonotic pathogens isolated from environmental sites in companion animal veterinary hospitals in southern ontario.hospital-based infection control in veterinary medicine is emerging and the role of the environment in hospital-acquired infections (hai) in veterinary hospitals is largely unknown. this study was initiated to determine the recovery of escherichia coli and selected veterinary and zoonotic pathogens from the environments of 101 community veterinary hospitals. the proportion of hospitals with positive environmental swabs were: e. coli--92%, clostridium difficile--58%, methicillin-resistant staphyl ...201021119862
antibiotic resistance profiles of staphylococcus pseudintermedius isolates from canine patients in korea.in this study, the prevalence of antibiotic resistance was examined among 74 staphylococcus pseudintermedius strains recently isolated from clinical cases of canine pyoderma and otitis externa at the veterinary teaching hospital at konkuk university, korea. bacterial resistance to the nine commonly used antibiotics was evaluated by a standard disk diffusion technique based on the guidelines of the clinical and laboratory standards institute. the results demonstrated that most s. pseudintermedius ...201021193835
prevalence of canine methicillin resistant staphylococcuspseudintermedius in a veterinary diagnostic laboratory in italy.the overall prevalence of methicillin-resistant staphylococcus pseudintermedius (mrsp) was 2% (10/590) among 590 canine specimens submitted to an italian veterinary diagnostic laboratory during a two-month period, and 21% (10/48) among staphylococcus intermedius group (sig) isolates. all methicillin-resistant strains exhibited additional resistance to fluoroquinolones, gentamicin, lincosamides, tetracyclines, and potentiated sulfonamides, belonged predominantly to spa type t02 and harboured sccm ...201020971485
catheter-related bacteremia caused by staphylococcus pseudintermedius refractory to antibiotic-lock therapy in a hemophilic child with dog exposure.we describe a case of catheter-related bacteremia due to staphylococcus pseudintermedius in a child with dog exposure. the organism was confirmed as s. pseudintermedius based on 16s rrna gene sequence analysis and positive pcr-restriction fragment length polymorphism of the pta gene.201020164279
a veterinary perspective on methicillin-resistant staphylococci.to familiarize the reader with the epidemiology, diagnosis, and infectious and zoonotic potential of methicillin-resistant staphylococci.201020230433
coryneform bacteria associated with canine otitis externa.this study aims to investigate the occurrence of coryneform bacteria in canine otitis externa. a combined case series and case-control study was carried out to improve the current knowledge on frequency and clinical significance of coryneform bacteria in samples from canine otitis externa. a total of 16 cases of otitis externa with involvement of coryneform bacteria were recorded at two referral veterinary hospitals in denmark and the us, respectively. coryneform bacteria were identified by part ...201020434850
widespread rapid emergence of a distinct methicillin- and multidrug-resistant staphylococcus pseudintermedius (mrsp) genetic lineage in europe.in order to gain a deeper insight into the phylogenetic background and diversity of methicillin-resistant s. pseudintermedius (mrsp) of animal origin, genetic relationships and clonal distribution among 146 european mrsp were examined using different molecular and phenotypical typing approaches. mrsp strains were derived from clinical microbiological specimens (mainly of small animal origin) sent in for diagnostic purposes from various veterinary facilities between 2005 and 2008. pulsed-field ge ...201020181441
proposed changes to clinical laboratory standards institute interpretive criteria for methicillin-resistant staphylococcus pseudintermedius isolated from dogs. 201020093710
methicillin-resistant staphylococci isolated from healthy horses and horse personnel in italy.methicillin-resistant staphylococci (mrs) were isolated from nasal swabs of 56 of 159 (35.2%; 95% confidence interval [ci]: 27.9-43.2%) healthy horses. two nasal swabs were collected from each horse; 43 of 159 (27%; 95% ci: 20.5-34.8%) of the cohort were colonized by mrs strains in 1 nostril, while in the remaining 13 of 159 (8.2%; 95% ci: 4.6-13.9%), different or identical mrs strains were isolated in both nostrils. of the 29 humans in close contact with the horses tested, 4 (13.8%; 95% ci: 4.5 ...201020093688
in vitro antimicrobial activity of a commercial ear antiseptic containing chlorhexidine and tris-edta.minimum bactericidal concentrations (mbcs) of a commercial ear antiseptic containing chlorhexidine 0.15% and tris-edta (otodine) were determined by broth microdilution for 150 isolates representing the most common pathogens associated with canine otitis. the microorganisms were classified into three groups according to their levels of susceptibility. the most susceptible group included staphylococcus pseudintermedius, malassezia pachydermatis, streptococcus canis and corynebacterium auriscanis, ...201020030799
treatment of a lower urinary tract infection in a cat caused by a multi-drug methicillin-resistant staphylococcus pseudintermedius and enterococcus faecalis.staphylococci and enterococci are common causes of urinary tract infections in cats. however, both species are rarely implicated together as causes of lower urinary tract infections associated with urethral obstruction. this report describes the first case of a multi-drug methicillin-resistant staphylococcus pseudintermedius belonging to spa type t06 and enterococcus faecalis urinary infection in a cat with pre-existing and recurrent urethral obstruction. both species were isolated at >10(5)cfu/ ...201020609607
the first case of a staphylococcus pseudintermedius infection after joint prosthesis implantation in a dog.we have reported a bacterial infection in a dog with progressive dysplasia of the hips. orthopedic surgery was performed. seven weeks prior to the surgery, the patient was bitten by another dog. the postimplantation wound exuded for four days after the surgery. microbiological analysis performed by standard identification techniques showed the presence of staphylococcus intermedius, but an additional molecular analysis indicated s. pseudintermedius. this was followed by an evaluation of antibiot ...201020734760
methicillin-resistant staphylococcus aureus and staphylococcus pseudintermedius in veterinary medicine.staphylococci are important opportunistic pathogens in most animal species. among the most relevant species are the coagulase positive species staphylococcus aureus and staphylococcus pseudintermedius. methicillin resistance has emerged as an important problem in both of these organisms, with significant concerns about animal and public health. the relative importance of these staphylococci on different animal species varies, as do the concerns about zoonotic transmission, yet it is clear that b ...201019246166
in vitro comparison of staphylococcus pseudintermedius susceptibility to common cephalosporins used in dogs.the in vitro activity of 10 cephalosporin antimicrobial agents against 75 isolates of methicillin-susceptible staphylococcus pseudintermedius derived from dogs was assessed. the lowest minimal inhibitory concentration for 90% of strains (mic90) values obtained were for cephalothin, cefovecin, and cefazolin (0.12 ug/ml), followed by ceftiofur and cefoxitin (0.25 ug/ml), cefpodoxime (0.5 ug/ml), and cefaclor and cefadroxil (1 ug/ml). the highest mic90 values were found for cephalexin and cefixime ...201020960413
coagulase-positive staphylococcus pseudintermedius from animals causing human endocarditis.we report a case of infection with coagulase-positive staphylococcus pseudintermedius related to the implantation of a cardioverter-defribrillator device. this species is usually isolated from infected animals, and contact with a dog was the probable source of infection in this patient. this isolate produced a leukotoxin effective against human polymorphonuclear leukocytes.201021075051
an igg-binding protein a homolog in staphylococcus hyicus.shotgun phage display was used to identify a homolog of the igg-binding protein staphylococcal protein a in staphylococcus hyicus type strain ccug 15602/atcc 11249. this bacterium is the causative agent of exudative epidermitis in pigs and can also cause mastitis in cattle. a protein with similar features as the originally identified protein a in staphylococcus aureus was described; an ysirk-type signal peptide, four igg-binding domains, a putative peptidoglycan-binding domain, and a cell wall a ...201021111546
prevalence of methicillin-resistant staphylococcus pseudintermedius isolated from clinical samples of companion animals and equidaes.in this study the prevalence of methicillin-resistant staphylococcus pseudintermedius (mrsp) in clinical specimens of different animal species was defined by investigating a total of 16,103 clinical samples originating from veterinary facilities of five german federal states in 2007. of all samples examined, 72 were positive for mrsp, giving an overall prevalence of 0.45%. in clinical specimens originating from small animals the prevalence was 0.58% (n=67; dogs n=61 and cats n=6), while samples ...200919097710
tandem repeat sequence analysis of staphylococcal protein a (spa) gene in methicillin-resistant staphylococcus pseudintermedius.a putative staphylococcal protein a (spa) gene was discovered in the genome of staphylococcus pseudintermedius and used for developing a species-specific spa typing protocol. thirty-one clinical methicillin-resistant s. pseudintermedius (mrsp) isolates from dogs and cats in four countries were characterized by spa typing, pulsed-field gel electrophoresis (pfge) and staphylococcal cassette chromosome (sccmec) typing. the results indicated the occurrence of two mrsp clones that acquired distinct s ...200918995972
antimicrobial resistance in staphylococcus pseudintermedius in the norwegian dog population.the occurrence of antimicrobial resistance in staphylococcus pseudintermedius from skin and ear infections in a representative sample of dogs unexposed to antimicrobial treatment before sampling was examined. the obtained isolates were further examined for genetic polymorphism and genetic background of resistance. a total of 59 isolates of s. pseudintermedius originating from 96 samples of 91 dogs in five different regions in norway were included in this study. susceptibility testing was perform ...200919216647
quorum sensing in veterinary pathogens: mechanisms, clinical importance and future perspectives.under certain circumstances the individuals of a bacterial population may find advantages in acting together and making "collective decisions". this phenomenon is better known as quorum sensing. when the concentration of signal molecules produced by the surrounding bacteria exceeds a certain threshold, the bacterial population acts as a single organism, collectively expressing virulence genes, biofilm forming genes, etc. several mechanisms of quorum sensing are discussed, each with its distinct ...200919185433
evaluation of susceptibility test breakpoints used to predict meca-mediated resistance in staphylococcus pseudintermedius isolated from dogs.clinical and laboratory standards institute interpretive breakpoints for in vitro susceptibility tests that predict meca-mediated oxacillin resistance in staphylococcus pseudintermedius isolates from animals have been changed twice in the past decade. moreover, there are no counterpart recommendations for human isolates of s. pseudintermedius. individual medical and veterinary laboratories variably use interpretive breakpoints identical to those recommended for use with staphylococcus aureus or ...200919139501
identification of a predominant multilocus sequence type, pulsed-field gel electrophoresis cluster, and novel staphylococcal chromosomal cassette in clinical isolates of meca-containing, methicillin-resistant staphylococcus pseudintermedius.methicillin resistance encoded by the meca gene is increasingly observed in staphylococcus pseudintermedius. little is known about the population genetics of veterinary staphylococci bearing methicillin resistance. the aim of this study was to determine the relatedness of resistant bacteria and to compare them with methicillin-susceptible isolates. multilocus sequence typing (mlst) and pulsed-field gel electrophoresis (pfge) fragment profiling were performed on methicillin-resistant s. pseudinte ...200919604657
inducible clindamycin-resistance in methicillin-resistant staphylococcus aureus and methicillin-resistant staphylococcus pseudintermedius isolates from dogs and cats. 200919592178
staphylococcus pseudintermedius expresses surface proteins that closely resemble those from staphylococcus aureus.staphylococcus pseudintermedius is a commensal of dogs that is implicated in the pathogenesis of canine pyoderma. this study aimed to determine if s. pseudintermedius expresses surface proteins resembling those from staphylococcus aureus and to characterise them. s. pseudintermedius strain 326 was shown to adhere strongly to purified fibrinogen, fibronectin and cytokeratin 10. it adhered to the alpha-chain of fibrinogen which, along with binding to cytokeratin 10, is the hallmark of clumping fac ...200919372010
staphylococcus pseudintermedius necrotizing fasciitis in a dog.staphylococcus pseudintermedius was implicated as the cause of rapidly progressive and fatal necrotizing fasciitis in a dog. the isolate was methicillin-susceptible and did not contain genes encoding the panton-valentine leukocidin. while streptococcus canis is typically considered to be the main cause of necrotizing fasciitis in dogs, staphylococci should also be considered.200919721787
coagulase positive staphylococcal colonization of humans and their household pets.staphylococcus aureus colonization was identified in 67/242 (28%) humans, 19/132 (14%) dogs, and 7/161 (4.3%) cats in households in ontario, with methicillin-resistant s. aureus present in 8 (3.3%) humans, 2 (1.5%) dogs, and 0 cats. staphylococcus pseudintermedius was isolated from 8 (4.1%) humans, 61 (46%) dogs, and 11 (6.8%) cats, with methicillin-resistant s. pseudintermedius detected in 1 (0.4%) human, 6 (4.5%) dogs, and 2 (1.2%) cats. staphylococcus schleiferi subspecies coagulans was isola ...200919949556
evaluation of clinical laboratory standards institute interpretive criteria for methicillin-resistant staphylococcus pseudintermedius isolated from dogs.the clinical and laboratory standards institute published in 2008 new interpretive criteria for the identification of methicillin resistance in staphylococci isolated from animals. the sensitivity of the 2008 interpretive criteria for meca gene-positive staphylococcus pseudintermedius, compared with the previous criteria of 2004, was investigated. thirty clinical isolates of methicillin-resistant s. pseudintermedius from dogs were used. the presence of the meca gene was determined by polymerase ...200919737765
occurrence of antimicrobial resistant bacteria in healthy dogs and cats presented to private veterinary hospitals in southern ontario: a preliminary study.the prevalence and patterns of antimicrobial susceptibility of fecal escherichia coli, salmonella spp., extended beta-lactamase producing e. coli (esbl-e. coli), methicillin-resistant staphylococcus aureus (mrsa), and methicillin-resistant staphylococcus pseudintermedius (mrsp) were determined for healthy dogs (n = 188) and cats (n = 39) from veterinary hospitals in southern ontario that had not had recent exposure to antimicrobials. the prevalence of antimicrobial resistance in e. coli was as f ...200920046603
staphylococcus pseudintermedius versus staphylococcus intermedius. 200918760884
molecular diagnostic identification of staphylococcus pseudintermedius.we report the first diagnostic test for the identification of staphylococcus pseudintermedius involving a simple pcr-restriction fragment length polymorphism approach. the method allows discrimination of s. pseudintermedius from the closely related members of the staphylococcus intermedius group and other important staphylococcal pathogens of humans and dogs.200919091817
staphylococcal colonization of mucosal and lesional skin sites in atopic and healthy dogs.staphylococcal colonization was compared in healthy dogs and in dogs with atopic dermatitis. bacterial swabs were collected from the nasal mucosa, ear and perineum of 43 healthy and 24 atopic dogs and also from potentially infected skin lesions of the atopic dogs. coagulase positive staphylococcal isolates were identified to the species level. at the time of this study staphylococcus intermedius was considered a single species but has since been recognized as comprising at least three species wi ...200919392768
risk of colonization or gene transfer to owners of dogs with meticillin-resistant staphylococcus pseudintermedius.to determine the zoonotic risk from meticillin-resistant staphylococcal species or transfer of resistance genes between dogs with pyoderma and their owners, 25 dog-owner pairs were studied. cultures were obtained from the dog's lesions and the owner's nasal cavity on the initial visit. staphylococcus isolates were identified and antimicrobial susceptibility tests were performed by the kirby-bauer disk diffusion method. presence of the meca gene was determined by pcr. staphylococcal cassette chro ...200920178487
the staphylococcus intermedius group of bacterial pathogens: species re-classification, pathogenesis and the emergence of meticillin resistance.staphylococcus intermedius has been long regarded as the common cause of pyoderma in dogs and other animals. despite its clinical importance, our understanding of the population genetics and pathogenesis of s. intermedius is limited. this review summarizes recent studies which have explored the genetic diversity of s. intermedius isolates resulting in a re-classification of the species and the identification of staphylococcus pseudintermedius as the common canine pyoderma pathogen. also discusse ...200920178486
identification of first exfoliative toxin in staphylococcus pseudintermedius.staphylococcus aureus, staphylococcus hyicus, and staphylococcus chromogenes are known to cause skin infections in human or animals by producing exfoliative toxins (ets). staphylococcus pseudintermedius can also cause canine pyoderma, but no exfoliative toxins or similar toxins have been reported. pcr with degenerate primers targeted to the conserved regions in eta, etb, and etd from s. aureus and shetb from s. hyicus, and subsequent chromosome walking identified a novel gene, designated as exi ...200919891731
infection with methicillin-resistant staphylococcus pseudintermedius masquerading as cefoxitin susceptible in a dog.a 2-year-old dog was evaluated because of complications that developed following tibial plateau leveling osteotomy. infection of the surgical site developed following removal of the failed implant.200919878005
a novel member of the thermolysin family, cloning and biochemical characterization of metalloprotease from staphylococcus pseudintermedius.thermolysins constitute a family of secreted bacterial metalloproteases expressed, among others, by several pathogens. strains of staphylococcus pseudintermedius isolated from diseased dogs and judged as protease-positive, by skim milk agar plate culture, were investigated for protease content. no proteolytic activity was detected when the bacteria were grown in regular liquid media. unexpectedly, supplementation of the medium with calcium ions resulted in expression of a metalloprotease and pro ...200818769737
methicillin-resistant staphylococcal colonization in dogs entering a veterinary teaching hospital.nasal, axillary and rectal swabs were collected from 193 dogs admitted to the ontario veterinary college veterinary teaching hospital. enrichment culture was performed and coagulase positive staphylococci were identified via standard methods. methicillin-resistant staphylococcus pseudintermedius was isolated from 4/193 (2.1%) dogs, and methicillin-resistant staphylococcus aureus and methicillin-resistant staphylococcus schleiferi subsp. coagulans were each isolated from 1/193 (0.5%) dogs. methic ...200817643874
methicillin-resistant staphylococcus aureus and staphylococcus pseudintermedius detected in the bft-germvet monitoring programme 2004-2006 in germany.during recent years, methicillin-resistant staphylococcus aureus (mrsa) strains from animals have become the focus of various studies. in the present study, coagulase-positive staphylococci obtained from pigs, dogs and cats suffering from acute infections, which had been collected in the bft-germvet monitoring programme in germany and phenotypically identified as oxacillin-resistant, were characterized.200818096559
characterization of new staphylococcal cassette chromosome mec (sccmec) and topoisomerase genes in fluoroquinolone- and methicillin-resistant staphylococcus pseudintermedius.fluoroquinolone- and methicillin-resistant staphylococcus pseudintermedius isolates harbor two new staphylococcal cassette chromosome mec (sccmec) elements that belong to class a, allotype 3 (sccmec ii-iii), and to the new allotype 5 (sccmec vii). analysis of the complete nucleotide sequences of the topoisomerase loci gyrb/gyra and grlb/grla revealed mutations involved in fluoroquinolone resistance.200818305127
emergence of methicillin-resistant staphylococcus pseudintermedius in switzerland: three cases of urinary tract infections in cats.methicillin resistance has emerged in clinical isolates of staphylococcus pseudintermedius from cats in switzerland. three cats suffering from urinary tract infections were infected with methicillin-resistant s. pseudintermedius (mrsp). phenotypic and genotypic characterization of the resistance profile showed that the isolates displayed resistance to all beta-lactams and cephalosporins (blaz, meca), fluoroquinolones, tetracyclines [tet(k)], macrolides, lincosamides and streprogramins b [erm(b)] ...200818714937
in vitro activity of fusidic acid and mupirocin against coagulase-positive staphylococci from pets.methicillin-resistant staphylococcus aureus (mrsa) and multiresistant staphylococcus pseudintermedius (mrsp) have emerged as important pathogens in animal infections. associated therapeutic problems and the zoonotic potential of staphylococci have renewed interest in topical antibiotics for treatment and carrier decolonization. fusidic acid and mupirocin are used topically in humans and animals but resistant strains isolated from people are increasing. this study investigates the in vitro activi ...200818819974
population genetic structure of the staphylococcus intermedius group: insights into agr diversification and the emergence of methicillin-resistant strains.the population genetic structure of the animal pathogen staphylococcus intermedius is poorly understood. we carried out a multilocus sequence phylogenetic analysis of isolates from broad host and geographic origins to investigate inter- and intraspecies diversity. we found that isolates phenotypically identified as s. intermedius are differentiated into three closely related species, s. intermedius, staphylococcus pseudintermedius, and staphylococcus delphini. s. pseudintermedius, not s. interme ...200717905991
methicillin-resistant staphylococcus pseudintermedius in a veterinary teaching hospital.we surveyed methicillin-resistant coagulase-positive staphylococcus (mrcps) strains from 57 (26 inpatient and 31 outpatient) dogs and 20 veterinary staff in a veterinary teaching hospital. from the staff, three mrcps strains were isolated, and two were methicillin-resistant staphylococcus aureus (mrsa). in contrast, 18 mrcps strains were detected in both inpatient (12 of 26 [46.2%]) and outpatient (6 of 31 [19.4%]) dogs. among them, only one strain was mrsa. using direct sequencing of soda and h ...200717267624
reclassification of phenotypically identified staphylococcus intermedius strains.to reclassify phenotypically identified staphylococcus intermedius strains, which might include true s. intermedius strains and novel species such as staphylococcus pseudintermedius and staphylococcus delphini, we analyzed molecular phylogenies and phenotypic characteristics of 117 s. intermedius group (sig) strains tentatively identified as being s. intermedius by the rapid id32 staph assay. from phylogenetic analyses of soda and hsp60 sequences, the sig strains were divided into three clusters ...200717596353
first case of staphylococcus pseudintermedius infection in a human.we present the first clinical report of a staphylococcus pseudintermedius infection in a human. biochemically, s. pseudintermedius can be easily misidentified as s. aureus. therefore, the final microbiological identification requires the combination of phenotypic and genotypic tests.200617050817
staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals.four staphylococcal isolates from clinical and necropsy specimens from a cat, a dog, a horse and a parrot (psittacus erithacus timneh) were found to constitute a distinct taxon. 16s rrna gene sequence analysis revealed that its closest phylogenetic relatives are staphylococcus intermedius and staphylococcus delphini. growth characteristics, biochemical features and dna-dna hybridizations demonstrated that the strains differ from these and other known species and that they represent a single, nov ...200516014483
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