Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
methicillin-resistant staphylococcus pseudintermedius in rats. 201323260061
feasibility of magnetic resonance electrical impedance tomography (mreit) conductivity imaging to evaluate brain abscess lesion: in vivo canine model.to show the feasibility of magnetic resonance electrical impedance tomography (mreit) conductivity imaging in terms of its capability to provide new contrast information of abscess lesion and characterize time-course variations before and after the induction of brain abscess.201323239506
in vitro adherence of staphylococcus pseudintermedius to canine corneocytes is influenced by colonization status of corneocyte donors.the current knowledge of in vitro adherence of staphylococcus pseudintermedius to canine corneocytes is limited to comparative analyses between strains, staphylococcal species or corneocytes collected from different breeds, body sites and hosts. however, the role played by colonization status of corneocyte donors remains unknown. the aim of this study was to evaluate the adherence properties of commensal s. pseudintermedius strains to corneocytes collected from dogs with different colonization s ...201323834238
an in vitro study to determine the minimal bactericidal concentration of sodium hypochlorite (bleach) required to inhibit meticillin-resistant staphylococcus pseudintermedius strains isolated from canine skin.meticillin-resistant staphylococcus pseudintermedius (mrsp) is becoming a more common pathogen in animals treated in veterinary hospitals, raising concerns for transmission and possible contamination of the hospital itself. the identification of effective agents for the decontamination of veterinary patients and hospitals is crucial. sodium hypochlorite (6.15%) or 'bleach' is an inexpensive and frequently used decontamination agent in human and veterinary hospitals and an adjunctive treatment fo ...201324118401
incidence of inducible clindamycin resistance in staphylococcus pseudintermedius from dogs.clindamycin is increasingly used to treat canine pyoderma. eight of 608 staphylococcus pseudintermedius isolates were positive for inducible clindamycin resistance by double-disk diffusion testing and pcr detection of ermb. staphylococcus pseudintermedius isolates that are erythromycin resistant but clindamycin susceptible by in vitro antimicrobial susceptibility testing should be tested for inducible clindamycin resistance.201324025915
a novel fexa variant from a canine staphylococcus pseudintermedius isolate that does not confer florfenicol resistance.transposon tn558 integrated in the chromosomal radc gene was detected for the first time in staphylococus pseudintermedius. it carried a novel fexa variant (fexav) that confers only chloramphenicol resistance. the exporter fexav exhibited two amino acid substitutions, gly33ala and ala37val, both of which seem to be important for substrate recognition. site-directed mutagenesis that reverted the mutated base pairs to those present in the original fexa gene restored the chloramphenicol-plus-florfe ...201323979755
novel pseudo-staphylococcal cassette chromosome mec element (ψsccmec57395) in methicillin-resistant staphylococcus pseudintermedius cc45.genetic characterization of methicillin-resistant staphylococcus pseudintermedius (mrsp) from thailand and israel revealed the presence of a predominant atypical clonal lineage which was not typeable by smai-pfge and sccmec typing. all the atypical isolates (n = 34) belonged to cc45 (30 st45 and 2 st179 isolates, 1 st57 isolate, and 1 st85 isolate). the isolates originated from healthy and diseased dogs and cats, as well as from the environment of one clinic. cfr9i-pulsed-field gel electrophores ...201323979735
pharmacodynamics of doxycycline and tetracycline against staphylococcus pseudintermedius: proposal of canine-specific breakpoints for doxycycline.doxycycline is a tetracycline that has been licensed for veterinary use in some countries, but no clinical breakpoints are available for veterinary pathogens. the objectives of this study were (i) to establish breakpoints for doxycycline and (ii) to evaluate the use of tetracycline as a surrogate to predict the doxycycline susceptibility of staphylococcus pseudintermedius isolates. mics and inhibition zone diameters were determined for 168 canine s. pseudintermedius isolates according to clinica ...201323966509
pathogenicity potential of staphylococcus pseudintermedius strains isolated from canine carriers and from dogs with infection signs. 201323328490
adverse effects of rifampicin in dogs and serum alanine aminotransferase monitoring recommendations based on a retrospective study of 344 dogs.rifampicin has been reported to have potent activity against staphylococci, including meticillin-resistant staphylococcus pseudintermedius. there is limited documented information regarding adverse effects and recommendations for serum biochemistry monitoring.201324164464
hospital-associated meticillin-resistant staphylococcus pseudintermedius in a french veterinary hospital. 201327873618
misuse of antimicrobials and selection of methicillin-resistant staphylococcus pseudintermedius strains in breeding kennels: genetic characterization of bacteria after a two-year interval.methicillin-resistant staphylococcus pseudintermedius (mrsp) strains have been isolated from dogs with increasing frequency; prolonged or excessive use of antimicrobials is associated with the selection of mrsp, and misuse of antimicrobials is frequent in breeding kennels. this study was carried out in two breeding kennels (a and b) in which we had isolated mrsp in 2008: the aim was to assess colonization of previously positive bitches and of other bitches sharing the same environment and to ass ...201322551469
multilocus sequence typing for characterization of staphylococcus pseudintermedius.staphylococcus pseudintermedius is an opportunistic pathogen in dogs. four housekeeping genes with allelic polymorphisms were identified and used to develop an expanded multilocus sequence typing (mlst) scheme. the new seven-locus technique shows s. pseudintermedius to have greater genetic diversity than previous methods and discriminates more isolates based upon host origin.201323115265
genome sequence of staphylococcus pseudintermedius strain e140, an st71 european-associated methicillin-resistant isolate.we report the first genome sequence of the methicillin-resistant staphylococcus pseudintermedius (mrsp) strain e140, isolated from a canine bite wound infection in denmark. this strain represents the dominant clonal lineage associated with canine mrsp infections in europe.201323516230
methicillin-resistant staphylococcus pseudintermedius infection in a bone marrow transplant recipient.staphylococcus pseudintermedius is a veterinary pathogen that has seldom been described as an agent of human disease. features of this probably underreported coagulase-positive staphylococcus species are depicted here through the description of a graft-versus-host disease-related wound infection caused by a multidrug-resistant strain.201323486715
successful treatment of encrusted cystitis associated with staphylococcus pseudintermedius infection in the urinary bladder of a dog.a 5-year-old female spayed mixed-breed dog was examined because of signs of persistent stranguria following treatment for urethral obstruction.201323445291
residual antibacterial activity of dog hairs after therapy with antimicrobial shampoos.topical antimicrobial treatment for canine pyoderma is becoming increasingly important, but little is known about the mechanism of action and persistence of activity of antimicrobial shampoos.201323432432
usefulness of cefovecin disk-diffusion test for predicting meca gene-containing strains of staphylococcus pseudintermedius and clinical efficacy of cefovecin in dogs with superficial pyoderma.cefovecin has been widely used to treat skin infections in dogs. the relationship of the cefovecin disk-diffusion test results to the presence of the meca gene and the clinical efficacy of cefovecin have not been fully evaluated.201323331693
in vitro antiseptic susceptibilities for staphylococcus pseudintermedius isolated from canine superficial pyoderma in japan.topical therapy, particularly with chlorhexidine, is becoming increasingly common as a treatment option for canine pyoderma; however, there are limited studies on the susceptibility of staphylococcus pseudintermedius to chlorhexidine compounds.201323331688
serum anti-staphylococcus pseudintermedius ige and igg antibodies in dogs with atopic dermatitis and nonatopic dogs.dogs and humans with atopic dermatitis (ad) are predisposed to colonization and recurrent infection with staphylococcus spp. studies in humans suggest that staphylococcus-specific immunoglobulin e (ige) plays a key role in disease pathogenesis. few such studies have been undertaken in dogs.201323331675
evaluation of minocycline susceptibility of methicillin-resistant staphylococcus pseudintermedius.methicillin-resistant staphylococcus pseudintermedius (mrsp) is an increasingly important cause of opportunistic infections in dogs and cats. treatment options are often limited because of the high level of antimicrobial resistance. doxycycline resistance is common, but variable mechanisms of doxycycline resistance exist, with some conferring resistance to doxycycline but not minocycline. however, there has been limited study of minocycline susceptibility in clinical mrsp isolates nor has the as ...201323107659
transcription of canine toll-like receptor 2, β-defensin 1 and β-defensin 103 in infected atopic skin, non-infected atopic skin, healthy skin and the cpek cell line.dogs and humans with atopic dermatitis (ad) have a high prevalence of recurrent staphylococcal pyoderma. β-defensins (bds) and toll-like receptors (tlrs) are important in innate immunity against bacterial skin infections, and decreased bd and tlr2 expression has been associated with human ad. however, findings from recent studies of bd expression in human and canine ad have been variable and contradictory. the aim of this study was to further our understanding of the role of antimicrobial protei ...201323067725
in vitro evaluation of topical biocide and antimicrobial susceptibility of staphylococcus pseudintermedius from dogs.staphylococcus pseudintermedius is an important canine pathogen, and the emergence and widespread dissemination of meticillin-resistant strains (mrsp) is of significant concern. multidrug-resistant infections may require alternative approaches, such as the use of topical therapy. there is minimal information about the in vitro susceptibility of meticillin-susceptible s. pseudintermedius (mssp) and mrsp to biocides and topical antimicrobials.201223050904
multi-resistant staphylococcus pseudintermedius: a wake-up call in our approach to bacterial infection. 201222931397
methicillin-resistant staphylococcus pseudintermedius in sweden.staphylococcus pseudintermedius is an opportunistic pathogen that is one of the most frequent causes of infections in dogs. in europe, there are increasing reports of methicillin-resistant s. pseudintermedius (mrsp), and in sweden, mrsp has also been more frequently isolated during recent years. however, there is limited knowledge regarding the epidemiology and genetic relationship among the swedish isolates. this study therefore investigated the genetic relationship of mrsp isolated from compan ...201222931056
prevalence and characterization of methicillin-resistant staphylococcus pseudintermedius in pets from south china.the aim of this study was to determine the presence of and characterize methicillin-resistant staphylococcus pseudintermedius (mrsp) isolated from pets in south china. from 2007 to 2009, 898 samples were collected from 785 pets in guangdong province. the identity of staphylococcal species and the presence of methicillin resistance were confirmed by phenotypic and genotypic assays. the genetic relationships of mrsp isolates were determined by multilocus sequence typing (mlst), pfge and spa typing ...201222770517
treatment outcome of dogs with meticillin-resistant and meticillin-susceptible staphylococcus pseudintermedius pyoderma.the prevalence of meticillin- and multidrug-resistant staphylococcus pseudintermedius (mrsp) in canine pyoderma has been increasing in recent years; thus, treatment of these cases has become challenging.201222364681
clonal diversity and biofilm-forming ability of methicillin-resistant staphylococcus pseudintermedius.the aim of this study was to investigate the clonal epidemiology of methicillin-resistant staphylococcus pseudintermedius (mrsp) isolates from dogs in norway and to evaluate the antimicrobial resistance patterns and determine the biofilm-forming abilities of the isolates.201222258925
meticillin-resistant staphylococcus pseudintermedius: clinical challenge and treatment options.meticillin-resistant staphylococcus pseudintermedius (mrsp) has emerged as a major therapeutic challenge for small animal veterinarians over the past 10 years and continues to spread worryingly in many countries. this review focuses on the clinical aspects of mrsp infections seen in patients with skin disease and on currently available treatment options. in addition, it discusses the implications for in-contact people, other animals and the environment, because infection control strategies are l ...201222486942
carriage of methicillin-resistant staphylococcus pseudintermedius in dogs--a longitudinal study.methicillin-resistant s. pseudintermedius strains (mrsp) are reported with increasing frequency in bacterial cultures from dogs. the objectives of this study were to determine whether mrsp could be found in dogs several months after a clinically apparent infection and whether the length of carriage varied depending on systemic antimicrobial treatment, diagnosis at time of the first positive mrsp culture and the presence of skin disease or wounds. thirty-one dogs previously diagnosed with a clini ...201222444911
comparative mutant prevention concentration and mechanism of resistance to veterinary fluoroquinolones in staphylococcus pseudintermedius.the problem of antibacterial drug resistance is increasing worldwide, in part due to the therapeutic concentrations currently used based on the minimal inhibitory concentration (mic) as a measure of potency are often the very concentrations required to selectively enrich the resistant mutant portion of the population. a mutant prevention concentration (mpc)-based dosing strategy is suggested to improve the therapeutic outcome based on the mic.201222409306
antibiotic treatments of a methicillin-resistant staphylococcus pseudintermedius infection in a dog: a case presentation. 201222378055
staphylococcus pseudintermedius colonization patterns and strain diversity in healthy dogs: a cross-sectional and longitudinal study.this is the first large-scale study of staphylococcus pseudintermedius colonization and diversity in healthy dogs where samples were collected over a long time and strains were identified by pcr according to the current taxonomy of the s. intermedius group and typed by a highly discriminatory method such as pulsed field gel electrophoresis (pfge). a cross-sectional study of nasal, oral, perineal and inguinal carriage in 119 healthy dogs was followed by a longitudinal study where oral and perinea ...201222770516
factors associated with methicillin-resistant versus methicillin-susceptible staphylococcus pseudintermedius infection in dogs.to compare methicillin-resistant staphylococcus pseudintermedius (mrsp) and methicillin-susceptible s pseudintermedius (mssp) infections in dogs.201222657928
selection of antibiotics for meticillin-resistant staphylococcus pseudintermedius: time to revisit some old drugs?the aim of this review is to consider systemic therapy options for meticillin-resistant staphylococcus pseudintermedius (mrsp). infections caused by mrsp in small animals--particularly dogs--have been frustrating veterinarians in recent years. after a susceptibility test is performed, veterinarians are left to select from drugs that have not been frequently encountered on a susceptibility report. some of these are old drugs that have not been used regularly by veterinary dermatologists. as mrsp ...201222313056
comparative activity of pradofloxacin and marbofloxacin against coagulase-positive staphylococci in a pharmacokinetic-pharmacodynamic model based on canine pharmacokinetics.pradofloxacin (pra), a novel veterinary 8-cyano-fluoroquinolone (fq), is active against staphylococcus pseudintermedius, the primary cause of canine pyoderma. an in vitro pharmacokinetic-pharmacodynamic model was used to compare the activities of pra and marbofloxacin (mar) against three clinical isolates of s. pseudintermedius and reference strain staphylococcus aureus atcc 6538. experiments were performed involving populations of 10(10) cfu corresponding to an inoculum density of approximately ...201222276681
comparative genomic analysis of the genus staphylococcus including staphylococcus aureus and its newly described sister species staphylococcus simiae.staphylococcus belongs to the gram-positive low g + c content group of the firmicutes division of bacteria. staphylococcus aureus is an important human and veterinary pathogen that causes a broad spectrum of diseases, and has developed important multidrug resistant forms such as methicillin-resistant s. aureus (mrsa). staphylococcus simiae was isolated from south american squirrel monkeys in 2000, and is a coagulase-negative bacterium, closely related, and possibly the sister group, to s. aureus ...201222272658
comparative genomics of the staphylococcus intermedius group of animal pathogens.the staphylococcus intermedius group consists of three closely related coagulase-positive bacterial species including s. intermedius, staphylococcus pseudintermedius, and staphylococcus delphini. s. pseudintermedius is a major skin pathogen of dogs, which occasionally causes severe zoonotic infections of humans. s. delphini has been isolated from an array of different animals including horses, mink, and pigeons, whereas s. intermedius has been isolated only from pigeons to date. here we provide ...201222919635
antimicrobial resistance of staphylococcus pseudintermedius.staphylococcus pseudintermedius, staphylococcus intermedius and staphylococcus delphini together comprise the s. intermedius group (sig). within the sig, s. pseudintermedius represents the major pathogenic species and is involved in a wide variety of infections, mainly in dogs, but to a lesser degree also in other animal species and humans. antimicrobial agents are commonly applied to control s. pseudintermedius infections; however, during recent years s. pseudintermedius isolates have been iden ...201222681522
occurrence of methicillin-resistant staphylococci in surgically treated dogs and the environment in a swedish animal hospital.to investigate whether hospitalised dogs treated surgically may become culture positive for methicillin-resistant staphylococcus pseudintermedius or methicillin-resistant staphylococcus aureus.201222747733
staphylococcus pseudintermedius in the dog: taxonomy, diagnostics, ecology, epidemiology and pathogenicity.the dog is the natural host of staphylococcus pseudintermedius. many research efforts are currently being undertaken to expand our knowledge and understanding of this important canine commensal and opportunistic pathogen. the objective of this review is to summarize the current knowledge of the species, including the latest research outcomes, with emphasis on taxonomy, diagnostics, ecology, epidemiology and pathogenicity. despite the important taxonomic changes that have occurred over the past f ...201222515504
efficacy of clarithromycin on biofilm formation of methicillin-resistant staphylococcus pseudintermedius.surgical site infections (ssis) caused by biofilm-forming methicillin-resistant staphylococcus pseudintermedius (mrsp) have emerged as the most common hospital-acquired infections in companion animals. no methods currently exist for the therapeutic remediation of ssis caused by mrsp in biofilms. clarithromycin (cla) has been shown to prevent biofilm formation by staphylococcus aureus. this study aims to assess the in vitro activity of cla in eradicating mrsp biofilm formation on various material ...201223171620
methicillin-resistant staphylococcal contamination of cellular phones of personnel in a veterinary teaching hospital.hospital-associated infections are an increasing cause of morbidity and mortality in veterinary patients. with the emergence of multi-drug resistant bacteria, these infections can be particularly difficult to eradicate. sources of hospital-associated infections can include the patients own flora, medical staff and inanimate hospital objects. cellular phones are becoming an invaluable feature of communication within hospitals, and since they are frequently handled by healthcare personnel, there m ...201222533923
genetic and phenotypic characterization of methicillin-resistant staphylococci isolated from veterinary hospitals in south korea.staphylococci were isolated from veterinary staff, hospitalized animals, and medical equipment from 2 major tertiary veterinary hospitals in south korea to investigate antimicrobial resistance and genetic relatedness. the detection rate for staphylococci was 55.2% (111/201 samples), and 11 species were identified among the collected staphylococcal strains. the most prevalent species were staphylococcus pseudintermedius (52/111, 46.8%), staphylococcus epidermidis (21/111, 18.9%), and staphylococc ...201222529115
recovery of staphylococci from computer keyboards in a veterinary medical centre and the effect of routine cleaning.computers play a vital role in veterinary clinics for grading, examining results, updating records, giving discharge instructions and maintaining billing information. few studies have documented the degree of contamination or practical methods to disinfect computer equipment within the veterinary clinic setting. the intent of the present study was to characterise the frequency of recovery of staphylococcus species from computer keyboards from a veterinary teaching hospital setting and to evaluat ...201222447457
in vitro antimicrobial activity of miconazole and polymyxin b against canine meticillin-resistant staphylococcus aureus and meticillin-resistant staphylococcus pseudintermedius isolates.meticillin-resistant staphylococcus aureus (mrsa) and meticillin-resistant staphylococcus pseudintermedius (mrsp) infections are increasingly reported in dogs, and these bacteria may be isolated from ear infections.201222409321
prevalence of meticillin-resistant staphylococcus pseudintermedius (mrsp) from skin and carriage sites of dogs after treatment of their meticillin-resistant or meticillin-sensitive staphylococcal pyoderma.meticillin-resistant staphylococci are significant pathogens in veterinary dermatology, yet longitudinal studies of the impact of routine antimicrobial therapy on emergence or resolution of resistance are lacking.201222364707
case-control study of staphylococcus lugdunensis infection isolates from small companion animals.coagulase-negative staphylococcus lugdunensis has recently been shown to cause invasive infections of people, which are similar in pathogenic effect to those caused by staphylococcus aureus. little is known about the pathogenicity of s. lugdunensis in companion animals.201222862546
in vitro miconazole susceptibility of meticillin-resistant staphylococcus pseudintermedius and staphylococcus aureus.the emergence and dissemination of meticillin-resistant staphylococci has created significant treatment challenges in veterinary medicine and increased interest in topical therapy for superficial infections. concern has been expressed regarding the use of some topical antimicrobials in animals because of the potential for emergence of resistance, and additional options are required. miconazole has limited antibacterial properties that include antistaphylococcal activity.201222830415
evaluation of cefoxitin disk diffusion breakpoint for detection of methicillin resistance in staphylococcus pseudintermedius isolates from dogs.cefoxitin disk diffusion susceptibility testing is a recommended screening method for the detection of methicillin resistance in human isolates of staphylococcus aureus and coagulase-negative staphylococci. a retrospective analysis of 1,146 clinical isolates of staphylococcus pseudintermedius from dogs was conducted to determine if screening by the cefoxitin disk method can be similarly useful with s. pseudintermedius. the distribution of cefoxitin growth inhibition zone diameters within this co ...201222786971
antimicrobial resistance in bacteria from breeding dogs housed in kennels with differing neonatal mortality and use of antibiotics.this work examines the antimicrobial resistance of potentially pathogenic bacteria (staphylococcus pseudintermedius, streptococcus canis, escherichia coli) found in the vaginal tract in prepartum mammary secretions and postpartum milk of bitches housed in breeding kennels (n = 20; 92 bitches). the kennels were divided into three categories: no routine antimicrobial administration around parturition (category 1); routine administration of one antibiotic around parturition (category 2); routine ad ...201222898018
the in vitro activity of 15 antimicrobial agents against bacterial isolates from dogs.the in vitro activity of 15 antimicrobial agents against clinical isolates of staphylococcus pseudintermedius, staphylococcus aureus, escherichia coli, pasteurella spp. and streptococcus canis from dogs was investigated. for staphylococcus spp., the highest frequency of resistance was observed for penicillin, followed by ampicillin, tetracycline and chloramphenicol. the highest frequency of resistance in e. coli isolates was recorded for tetracycline and streptomycin. pasteurella spp. and s. can ...201222516694
[staphylococcus pseudintermedius and pasteurella dagmatis associated in a case of community-acquired pneumonia]. 201222366256
molecular characterization of high-level mupirocin resistance in staphylococcus pseudintermedius.the genetic analysis of high-level mupirocin resistance (hi-mup(r)) in a staphylococcus pseudintermedius isolate from a dog is presented. the hi-mup(r) iles2 gene flanked by a novel rearrangement of directly repeated insertion sequence is257 elements was located, together with the aminoglycoside resistance aaca-aphd determinant, on a conjugative plasmid related to the psk41/pgo1 family plasmids.201223269741
distribution of sccmec-associated phenol-soluble modulin in staphylococci.the recently described phenol-soluble modulin psm-mec was detected in staphylococcus aureus, staphylococcus epidermidis, staphylococcus fleuretti, staphylococcus hominis, staphylococcus pseudintermedius, staphylococcus saprophyticus, staphylococcus simulans and staphylococcus vitulinus from different hosts (humans, goats, dogs, cats, pigs, cattle and turkeys). it was identified in isolates harbouring sccmec types ii, iia, iib, iid, iii, viii and in some irregular or truncated elements.201222251619
what's happened to staphylococcus intermedius? taxonomic revision and emergence of multi-drug resistance.staphylococcus intermedius has been the predominant coagulase-positive staphylococcus isolated from canine skin and mucosae and the most commonly reported staphylococcal pathogen in small animal practice for the last 35 years. although microbiological tests have historically indicated variability in biochemical characteristics amongst s. intermedius isolates from animals, an acceptable level of diagnostic accuracy for clinical purposes was readily achievable with routine phenotypic testing. howe ...201222251285
methicillin-resistant staphylococcus pseudintermedius isolated from canine pyoderma in north china.aims:  to determine the prevalence of carriage of methicillin-resistant staphylococcus pseudintermedius (mrsp) among dogs with pyoderma from two small animal hospitals in north china during a 21-month period, and to characterize these isolates. methods and results:  swabs were taken from 260 dogs with pyoderma, the staphylococcal species isolated and methicillin resistance were confirmed phenotypically and genotypically. the identified mrsp isolates were characterized by multilocus sequence typi ...201222229826
activity, expression and genetic variation of canine β-defensin 103: a multifunctional antimicrobial peptide in the skin of domestic dogs.the skin functions as more than a physical barrier to infection. epithelial cells of the skin can synthesize antimicrobial peptides, including defensins, which exhibit direct antimicrobial activity. here we characterize the expression pattern, genetic variation and activity of the major β-defensin expressed in canine skin, canine β-defensin 103 (cbd103). the gene encoding cbd103 exhibits two forms of polymorphism: a common 3-basepair deletion allele and a gene copy-number variation. golden retri ...201222261569
detection of methicillin-resistant staphylococcus pseudintermedius with commercially available selective media.commercially available selective media for methicillin-resistant staphylococcus aureus (mrsa) were tested for the detection and isolation of methicillin-resistant staphylococcus pseudintermedius (mrsp).201222023239
necrotizing fasciitis caused by methicillin-resistant staphylococcus pseudintermedius at a previously irradiated site in a dog.a great dane dog was presented with a small, superficial wound on the left tarsus that rapidly progressed to a large necrotic area. the dog had undergone radiation therapy in the left tarsal region 33 months previously. necrotizing fasciitis was diagnosed on histopathological examination, and bacterial culture revealed methicillin-resistant staphylococcus pseudintermedius.201223633717
identification and antibiotic susceptibility of bacterial isolates from dogs with otitis externa in australia.to identify and quantify the five most frequently isolated significant bacterial microorganisms, and their antibiotic susceptibility, from bacterial cultures of samples taken from the ears of dogs with otitis externa.201223356371
evaluation of the bacterial microflora of the conjunctival sac of healthy dogs and dogs with atopic dermatitis.the aim of this case-control study was to evaluate and compare the bacterial microflora from the conjunctival sac of dogs with atopic dermatitis and healthy dogs. twenty-one atopic dogs without clinical and/or cytopathological signs of bacterial blepharoconjunctivitis and 21 breed-matched healthy dogs were enrolled. under topical anaesthesia, the inferior conjunctival sac of one eye was scraped twice. material was collected with a kimura spatula, spread over a slide and stained with a diff quick ...201121535255
antimicrobial resistance of staphylococcus pseudintermedius isolates from healthy dogs and dogs affected with pyoderma in japan.staphylococcus pseudintermedius strains were isolated from healthy dogs and dogs with pyoderma in 2000-2002 and 2009. all the isolates from dogs with pyoderma in 1999-2000 and from healthy dogs in 2000-2002 and 2009 were susceptible to cefalexin and/or other cephalosporins and oxacillin. however, 7.1-12.5 and 11.4% of s. pseudintermedius isolates from dogs with pyoderma in 2009 were resistant to cephalosporins and oxacillin, respectively. all s. pseudintermedius isolates from dogs with pyoderma ...201121745248
carriage of methicillin-resistant staphylococcus pseudintermedius in small animal veterinarians: indirect evidence of zoonotic transmission.methicillin-resistant staphylococcus pseudintermedius (mrsp) is increasingly reported in small animals and cases of human infections have already been described despite its recent emergence in veterinary practice. we investigated the prevalence of mrsp and methicillin-resistant staphylococcus aureus (mrsa) among small animal dermatologists attending a national veterinary conference in italy. nasal swabs were obtained from 128 veterinarians, seven of which harboured mrsp (nôçâ=ôçâ5; 3.9%) or mrsa ...201121824350
staphylococcus pseudintermedius exfoliative toxin exi selectively digests canine desmoglein 1 and causes subcorneal clefts in canine epidermis.staphylococcal exfoliative toxins are known to digest desmoglein (dsg) 1, a desmosomal cell-cell adhesion molecule, thus causing intraepidermal splitting in human bullous impetigo, staphylococcal scalded skin syndrome and swine exudative epidermitis. recently, a novel exfoliative toxin gene (exi), whose sequence shares significant homology with previously identified exfoliative toxins, was isolated from staphylococcus pseudintermedius. little is known about the pathogenic involvement of this tox ...201121410798
methicillin-resistant staphylococcus pseudintermedius among cats admitted to a veterinary teaching hospital. 201121703784
association between skin surface ph, temperature and staphylococcus pseudintermedius in dogs with immunomodulatory-responsive lymphocytic-plasmacytic pododermatitis.secondary bacterial infection is a frequent complication in lesional skin of dogs with immunomodulatory-responsive lymphocytic-plasmacytic pododermatitis (imr-lpp). however, the influence of skin ph and temperature in determining the composition of the cutaneous microflora at lesional sites has not been investigated. the association between imr-lpp and pedal skin temperature, ph and staphylococcus pseudintermedius isolates was thus evaluated. temperature and ph were measured in 20 dogs with imr- ...201121395884
prevalence, sites of colonization, and antimicrobial resistance among staphylococcus pseudintermedius isolated from healthy dogs in saskatoon, canada.in dogs, staphylococcus pseudintermedius is a common colonizer and is associated with pyoderma, otitis externa, and urinary tract infections. in the current study, nasal, pharyngeal, and rectal swabs were taken from 175 healthy dogs and cultured for s. pseudintermedius. the organism was found in 153 dogs (87.4%), including individuals in which it exclusively colonized in the nares (n ôçè=ôçè 1), pharynx (n ôçè=ôçè 16), and rectum (n ôçè=ôçè 17). antimicrobial susceptibility testing revealed that ...201121398462
complete genome sequence of the canine pathogen staphylococcus pseudintermedius.we report the first whole-genome sequence for a clinical isolate of staphylococcus pseudintermedius (ed99), the major pathogen responsible for canine bacterial pyoderma. s. pseudintermedius contains numerous mobile genetic elements and encodes an array of putative virulence factors, including superantigenic, cytolytic, and exfoliative toxins and cell wall-associated surface proteins.201121398539
serum pharmacokinetics of clindamycin hydrochloride in normal dogs when administered at two dosage regimens.the aim of this cross-over study was to compare clindamycin pharmacokinetics in the serum of clinically normal dogs when administered orally at two dosage regimens (5.5 mg/kg, twice daily, and 11 mg/kg, once daily), separated by a 1 week wash-out period. serum samples were obtained from six clinically normal laboratory beagles before, 3, 6, 9 and 12 h after the first and fifth dose of clindamycin at 5.5 mg/kg, twice daily, and before, 3, 6, 9, 12, 18 and 24 h after the first and third dose at 11 ...201121418348
molecular basis of rifampicin resistance in methicillin-resistant staphylococcus pseudintermedius isolates from dogs.methicillin-resistant staphylococcus pseudintermedius (mrsp) often display resistance to almost all classes of antimicrobial agents used in veterinary medicine. in the present study, we investigated the emergence of rifampicin resistance in mrsp, the persistence of these isolates and identified the corresponding mutations in the rpob gene.201121436154
in vitro antimicrobial activity of nitrofurantoin against escherichia coli and staphylococcus pseudintermedius isolated from dogs and cats.minimum inhibitory concentrations (mic) of nitrofurantoin were determined by agar dilution in 269 canine and feline isolates of escherichia coli and staphylococcus pseudintermedius, two of the most common bacterial species associated with urinary tract infection (uti) in small animals. the mic90 for e. coli and s. pseudintermedius were 32 and 16μg/ml, respectively. all isolates, including multidrug-resistant strains of known genetic background, displayed mics below the drug concentrations report ...201121482044
the role of meca and blaz regulatory elements in meca expression by regional clones of methicillin-resistant staphylococcus pseudintermedius.two major regional clones of methicillin-resistant staphylococcus pseudintermedius (mrsp) have been identified in europe and north america. they are designated multilocus sequence types (st) 71 and 68 and contain staphylococcal chromosome cassette (sccmec) types ii-iii and v(t), respectively. one notable difference between the two clones is a deletion in the meci/mecr1 regulatory apparatus of st 68 sccmec v(t). this deletion in analogous methicillin-resistant staphylococcus aureus (mrsa) results ...201121514752
urinary tract infection caused by methicillin-resistant staphylococcus pseudintermedius in a dog.a young neutered male pug dog was presented for evaluation of acute onset pollakiuria and hematuria. culture and susceptibility testing of urine identified a methicillin-resistant staphylococcus pseudintermedius, which was susceptible to only tetracycline among commonly used antimicrobials. treatment with doxycycline led to bacteriological cure and resolution of clinical signs.201121532822
expression and activity of a novel cathelicidin from domestic cats.cathelicidins are small cationic antimicrobial peptides found in many species including primates, mammals, marsupials, birds and even more primitive vertebrates, such as the hagfish. some animals encode multiple cathelicidins in their genome, whereas others have only one. this report identifies and characterizes feline cathelicidin (fecath) as the sole cathelicidin in domestic cats (felis catus). expression of fecath is predominantly found in the bone marrow, with lower levels of expression in t ...201121533281
comparative in vitro efficacy of antimicrobial shampoos: a pilot study.this study compared the antimicrobial efficacy of shampoos against meticillin-sensitive staphylococcus pseudintermedius (mssp), meticillin-resistant s. pseudintermedius (mrsp), antibiotic-sensitive pseudomonas aeruginosa (pa), multidrug-resistant p. aeruginosa (mdr-pa) and malassezia pachydermatis. three isolates were incubated for 10, 30 and 60 min with each shampoo diluted in phosphate-buffered saline. aliquots were then incubated for 16-18 h on sheep blood agar (bacteria) or for 3 days on sab ...201121777309
complete genome sequence of the veterinary pathogen staphylococcus pseudintermedius strain hku10-03, isolated in a case of canine pyoderma.staphylococcus pseudintermedius is a member of the coagulase-positive staphylococci and is the commonest cause of canine pyoderma. we report the first genome sequence of s. pseudintermedius, which shows the presence of numerous virulence factors akin to those of the related human pathogen staphylococcus aureus.201121278300
genetic relatedness of methicillin-resistant staphylococcus pseudintermedius (mrsp) isolated from a dog and the dog owner.in the present study four methicillin-resistant staphylococcus pseudintermedius (mrsp) strains isolated from a dog (n=3) and the anterior nares of the dog owner (n=1) were investigated by conventional and molecular methods. the species identity of the four s. pseudintermedius strains was confirmed by conventional methods, by pcr mediated amplification of s. intermedius/s. pseudintermedius specific segments of thermonuclease encoding gene nuc and by restriction fragment length polymorphism analys ...201121353270
genomic and surface proteomic analysis of the canine pathogen staphylococcus pseudintermedius reveals proteins which mediate adherence to the extracellular matrix.cell wall-associated (cwa) proteins made by gram-positive pathogens play a fundamental role in pathogenesis. staphylococcus pseudintermedius is a major animal pathogen responsible for the canine skin disease bacterial pyoderma. here we describe the bioinformatic analysis of the family of 18 predicted cwa proteins encoded in the genome of s. pseudintermedius strain ed99 and determine their distribution among a phylogenetically diverse panel of s. pseudintermedius clinical isolates, and closely-re ...201121576333
low rate of methicillin-resistant coagulase-positive staphylococcal colonization of veterinary personnel in hong kong.elevated rates of methicillin-resistant staphylococcus aureus (mrsa) carriage have been reported in veterinary personnel, suggesting an occupational colonization risk. hong kong veterinary personnel (n = 150) were sampled for coagulase-positive staphylococci (cps) nasal colonization. risk factors for colonization were assessed by questionnaire. isolates were identified and antibiotic susceptibility determined. all cps isolates were investigated for meca carriage, sccmec type and pvl genes. two s ...201119968853
transmission of methicillin-resistant staphylococcus pseudintermedius between infected dogs and cats and contact pets, humans and the environment in households and veterinary clinics.the objective of this study was to investigate the prevalence of methicillin-resistant staphylococcus pseudintermedius (mrsp) in people, pets and the environment in households with a pet with a clinical mrsp-infection within the past year. personnel and the environment at veterinary clinics were also screened. nasal swabs (humans), nasal and perineal swabs (pets) and environmental wipes were examined using selective culturing. twenty households were enrolled; 10/20 index cases still had clinical ...201121420256
Prevalence of methicillin-resistant staphylococci in northern Colorado shelter animals.Methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus pseudintermedius (MRSP) have been recognized as significant pathogens in veterinary medicine. There have been documented cases of MRSA infection and colonization in veterinary critical care units, in veterinary personnel, and in equine and feline patients. To date, there have been no studies examining the prevalence of MRSA or MRSP colonization of cats and dogs in animal shelters in the United States. The purpose of the curren ...201121908352
Bactericidal properties of pradofloxacin against veterinary pathogens.Pradofloxacin is a new veterinary 8-cyano-fluoroquinolone developed for use against bacterial infections in dogs and cats involving both aerobic and anaerobic bacteria. The minimal bactericidal concentrations have been determined against clinical isolates of Staphylococcus pseudintermedius, Staphylococcus aureus, Escherichia coli, Pasteurella multocida, Streptococcus canis, Proteus spp., Fusobacterium spp., Porphyromonas gingivalis and Prevotella species. A subset of these species was selected, ...201122209121
methicillin-resistant staphylococci (mrs) and extended-spectrum beta-lactamases (esbl)-producing enterobacteriaceae in companion animals: nosocomial infections as one reason for the rising prevalence of these potential zoonotic pathogens in clinical samples.the ongoing change in the relationship between humans and companion animals is hallmarked by the increasing intensive care provided to companion animals in veterinary medicine, resulting in growing numbers of high-risk animal patients. the emergence of nosocomial infections in small animal clinics is one of the major drawbacks of this development, especially in terms of multidrug-resistance and potentially zoonotic pathogens. this mini-review therefore addresses recent findings regarding the inc ...201122000738
review on methicillin-resistant staphylococcus pseudintermedius.staphylococcus pseudintermedius is an important opportunistic pathogen of companion animals, especially dogs. since 2006 there has been a significant emergence of methicillin-resistant s. pseudintermedius (mrsp) mainly due to clonal spread. this article reviews research on mrsp with a focus on occurrence, methods used for identification, risk factors for colonization and infection, zoonotic potential and control options. potential areas for future research are also discussed.201121930571
[pyoderma in dogs].bacterial infections associated with cutaneous commensal organisms like staphylococcus pseudintermedius are frequently encountered in small animal practice. in this review, pathogenesis, clinical signs, and topical and systemic therapeutic options are discussed.201122167166
meticillin-resistant strains of staphylococcus pseudintermedius in companion animals. 201121742696
bacterial contamination of commercial ear cleaners following routine home use.ear cleaning solutions are designed for repeated use, which raises the possibility for bacterial contamination leading to recurrent or persistent infectious otitis. the purpose of this study was to investigate the prevalence of bacterial contamination of commercial ear cleaners following routine home use in dogs and to describe the characteristics that are associated with contamination. used ear cleaner bottles and information regarding their use were obtained from canine owners visiting veterin ...201121645141
determination of enrofloxacin stability and in vitro efficacy against staphylococcus pseudintermedius and pseudomonas aeruginosa in four ear cleaner solutions over a 28 day period.chemical stability and in vitro bactericidal efficacy of 0.9% enrofloxacin-compounded solutions were evaluated following storage at room temperature for 28 days. chemical stability of enrofloxacin was determined by high-performance liquid chromatography (hplc) in five compounded solutions, including sterile water. bactericidal efficacy was determined by spiral plating serial 10-fold dilutions of bacteria and solutions followed by colony counts. tris-edta [trizedta(®) (te)], tris-edta and 0.15% c ...201121777310
heterogeneity of methicillin-sensitive staphylococcus pseudintermedius strains isolated from diseased dogs.thirty nine canine s. pseudintermedius strains were examined for antibiotic susceptibility and genetic polymorphisms. all strains were methicillin-sensitive s. pseudintermedius (mssp). resistance to penicillin was most prevalent (66.6%), followed by resistance to neomycin (56.4%), erythromycin (53.8%), clindamycin (48.7%), chloramphenicol (48.7%), and tetracycline (46.2%). pulsed-field electrophoresis (pfge) showed a high genetic polymorphism in the investigated strains.201121721415
longitudinal study on methicillin-resistant staphylococcus pseudintermedius in households.methicillin-resistant staphylococcus pseudintermedius (mrsp) is an emerging pathogen in dogs and has been found in europe, asia and north america. to date most studies are one-point prevalence studies and therefore little is known about the dynamics of mrsp in dogs and their surrounding. in this longitudinal study mrsp colonization in dogs and the transmission of mrsp to humans, contact animals and the environment was investigated. sixteen dogs with a recent clinical mrsp infection were included ...201122132141
detection and characterization of methicillin-resistant staphylococcus pseudintermedius in healthy dogs in la rioja, spain.the objective was to identify the methicillin-resistant coagulase-positive staphylococci (mrcops) nasal carriage rate of healthy dogs in la rioja (spain) and to characterize the recovered isolates by different molecular techniques. nasal samples from 196 dogs were obtained (98 household-dogs, 98 pound-dogs). isolates were identified and characterized by spa-, sccmec- and mlst-typing, smai-pfge, antimicrobial susceptibility, determination of antimicrobial resistance and toxin genes profiling. s. ...201121903268
In vitro killing of Escherichia coli, Staphylococcus pseudintermedius and Pseudomonas aeruginosa by enrofloxacin in combination with its active metabolite ciprofloxacin using clinically relevant drug concentrations in the dog and cat.Enrofloxacin is a fluoroquinolone antibacterial agent used to treat infections in companion animals. Enrofloxacin's antimicrobial spectrum includes Gram positive and Gram-negative bacteria and demonstrates concentration-dependent bacteriocidal activity. In dogs and cats, enrofloxacin is partially metabolized to ciprofloxacin and both active agents circulate simultaneously in treated animals at ratios of approximately 60-70% enrofloxacin to 30-40% ciprofloxacin. We were interested in determining ...201121925810
antimicrobial susceptibility of staphylococcus aureus and staphylococcus pseudintermedius isolated from various animals.this study characterized the antimicrobial susceptibility of 221 staphylococcus aureus isolated from various species, and 60 canine staphylococcus pseudintermedius isolated from 1986 through 2000 at the western college of veterinary medicine (wcvm). resistance of s. aureus was most common to penicillin (31%) and tetracycline (14%); resistance of s. pseudintermedius to penicillin was present in 8% and to tetracycline in 34% of isolates. resistance to trimethoprim/sulfamethoxazole was only seen am ...201121532820
Antibiotic treatments of a methicillin-resistant Staphylococcus pseudintermedius infection in a dog: a case presentation.We report the antibiotic treatments administered to a female dog with mastitis and successive pyoderma. Microbiological investigations allowed the identification of Staphylococcus pseudintermedius after 54 days of various antibiotic treatments. The isolate carried the mecA gene and was resistant to 9 of 15 tested antibiotics. Consistent antibiotic treatment of the infection was possible only after accurate microbiological diagnosis.201121866514
molecular characterization of staphylococcus pseudintermedius strains isolated from clinical samples of animal origin.the aim of this study was to determine the species distribution among 44 randomly selected clinical isolates (30 meca-positive and 14 meca-negative) of animal origin previously identified as staphylococcus intermedius by phenotypic tests and species-specific pcr amplification of the 16s rrna gene. for this purpose, we used a multiplex pcr for the detection of the nuc gene and restriction fragment length polymorphism analysis of pta gene amplified by pcr. both methods allow discrimination of stap ...201121874595
staphylococccus pseudintermedius surface proteins spsd and spso mediate adherence to ex vivo canine corneocytes.the gram-positive bacterium staphylococcus pseudintermedius is regarded as the major cause of canine bacterial pyoderma. despite its clinical importance, there is only very limited knowledge about the pathogenesis of s. pseudintermedius infection and the specific bacterial virulence factors involved in causing disease. using a whole-genome approach, we have previously identified 18 predicted cell-wall-anchored surface proteins representing possible virulence factors in a clinical isolate of s. p ...201122112246
antimicrobial activity of gallium maltolate against staphylococcus aureus and methicillin-resistant s. aureus and staphylococcus pseudintermedius: an in vitro study.gallium is a trivalent semi-metallic element that has shown antimicrobial activity against several important human pathogens. this antimicrobial activity is likely related to its substitution in important iron-dependent pathways of bacteria. the genus staphylococcus, which includes human and animal pathogens that cause significant morbidity and mortality, requires iron for growth and colonization. in this study, gallium maltolate, at various concentrations between 50 and 200μm, inhibited the in ...201121963417
insertion sequence is256 in canine pyoderma isolates of staphylococcus pseudintermedius associated with antibiotic resistance.staphylococcus pseudintermedius is the most frequent staphylococcal species isolated from canine pyoderma. the control of s. pseudintermedius infection is often difficult due to the expanded antimicrobial resistance phenotypes. antibiotic resistance in staphylococcal pathogens is often associated to mobile genetic elements such as the insertion sequence is256 that was first described as a part of the transposon tn4001, which confers aminoglycoside resistance in staphylococcus aureus and in staph ...201122261238
in vitro antimicrobial efficacy of β-defensin 3 against staphylococcus pseudintermedius isolates from healthy and atopic canine skin.β-defensins (bds) are highly conserved antimicrobial peptides important in innate defence against bacteria. β-defensin 3 has a specific role in protecting the skin. this study quantified the minimal inhibitory concentration (mic) of human (h)bd3 against staphylococcus pseudintermedius isolates from atopic and healthy dogs. single colony isolates (1 × 10(5) colony-forming units/ml log phase) were cultured with doubling dilutions of hbd3 in sodium phosphate buffer from 0.8 to 50 μg/ml at 37 °c for ...201020492622
Refresh
firstprev
1234
nextlast
Displaying items 201 - 300 of 363