Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| detection and sequence analysis of accessory gene regulator genes of staphylococcus pseudintermedius isolates. | staphylococcus pseudintermedius (sp) is the major pathogenic species of dogs involved in a wide variety of skin and soft tissue infections. the accessory gene regulator (agr) locus of staphylococcus aureus has been extensively studied, and it influences the expression of many virulence genes. it encodes a two-component signal transduction system that leads to down-regulation of surface proteins and up-regulation of secreted proteins during in vitro growth of s. aureus. the objective of this stud ... | 2015 | 27047173 |
| prevalence and risk factors for isolation of methicillin-resistant staphylococcus in dogs with keratitis. | to determine the prevalence of, and risk factors for, methicillin-resistant staphylococcus (mrs) isolation in dogs with naturally acquired bacterial keratitis. | 2015 | 25130050 |
| prevalence of methicillin-resistant staphylococcus spp. in the conjunctival sac of healthy dogs. | to determine the prevalence of selected coagulase-positive methicillin-resistant staphylococcus aureus (mrs) in the conjunctival sac in a group of healthy dogs and to compare the prevalence of ocular mrs colonization with colonization of typically assessed body sites including the nasal cavity and rectum. | 2015 | 24299189 |
| increasing antimicrobial resistance in clinical isolates of staphylococcus intermedius group bacteria and emergence of mrsp in the uk. | frequencies of antimicrobial resistance were determined amongst 14,555 clinical staphylococcus intermedius group (sig) isolates from uk dogs and cats to estimate resistance trends and quantify the occurrence of meticillin-resistant staphylococcus pseudintermedius (mrsp). reports from two diagnostic laboratories (13,313 general submissions, 1242 referral centre only submissions) were analysed retrospectively (2003/2006-2012). mrsp were defined by phenotypic resistance to meticillin and concurrent ... | 2015 | 25376505 |
| are staphylococcus intermedius infections in humans cases of mistaken identity? a case series and literature review. | staphylococcus intermedius and staphylococcus pseudintermedius are difficult to distinguish using conventional microbiological methods. molecular diagnostic tools change our understanding of the epidemiology of these 2 organisms. in this study, we present (1) a detailed review of the current literature on molecular diagnostics and (2) a case series in which misidentification was proven in 1 case. we conclude that s pseudintermedius is a more common human pathogen than previously recognized. | 2015 | 26509181 |
| an evaluation of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the identification of staphylococcus pseudintermedius isolates from canine infections. | it has been proposed, based on taxonomic and molecular studies, that all canine isolates belonging to staphylococcus intermedius group (sig) should be renamed staphylococcus pseudintermedius. however, isolates of sig and other coagulase-positive staphylococci share many phenotypic characteristics, which could lead to misidentification. the accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) for identifying s. pseudintermedius isolates obtained ... | 2015 | 25680922 |
| improved detection of staphylococcus intermedius group in a routine diagnostic laboratory. | the staphylococcus intermedius group (sig) includes zoonotic pathogens traditionally associated with dog bites. we describe a simple scheme for improved detection of sig using routine laboratory methods, report its effect on isolation rates, and use sequencing to confirm that, apart from one atypical sig strain, most isolates are staphylococcus pseudintermedius. | 2015 | 25502532 |
| characterisation of nasal staphylococcus delphini and staphylococcus pseudintermedius isolates from healthy donkeys in tunisia. | staphylococcus intermedius group (sig) bacteria can colonise the nares of some animals but are also emerging pathogens in humans and animals. | 2015 | 24913693 |
| [antimicrobial resistance and molecular epidemiology of staphylococcus pseudintermedius strains isolated from dog clinical samples]. | twenty-eight strains isolated from dog clinical samples identified as staphylococcus pseudintermedius by mass spectrometry (maldi-tof) were studied to assess antimicrobial susceptibility by the diffusion method and clonal relationship by pulsed field gel electrophoresis (pfge). methicillin resistance (3/28 isolates; 10,7%) was evaluated by meca pcr. fifteen strains (53.6%) were resistant to at least one of the antibiotics tested, and eleven of them (39.3%) showed multiple resistance (3 or more a ... | 2015 | 26323373 |
| evaluation of canine-specific minocycline and doxycycline susceptibility breakpoints for meticillin-resistant staphylococcus pseudintermedius isolates from dogs. | using the us clinical and laboratory standards institute (clsi) human tetracycline breakpoints to predict minocycline and doxycycline susceptibility of staphylococcus pseudintermedius (sp) isolates from dogs is not appropriate because they are too high to meet pharmacokinetic/pharmacodynamic data using a standard dose. new breakpoints have been approved for doxycycline and proposed for minocycline. revised breakpoints are four dilutions lower than tetracycline breakpoints, providing a more conse ... | 2015 | 26216025 |
| coagulase-positive staphylococcus: prevalence and antimicrobial resistance. | staphylococcus pseudintermedius is the most prevalent coagulase-positive staphylococcus inhabitant of the skin and mucosa of dogs and cats, causing skin and soft tissue infections in these animals. in this study, coagulase-positive staphylococcus species were isolated from companion animals, veterinary professionals, and objects from a clinical veterinary environment by using two particular culture media, baird-parker rpf agar and chromagar staph aureus. different morphology features of colonies ... | 2015 | 26535454 |
| genotypic relatedness and characterization of staphylococcus pseudintermedius associated with post-operative surgical infections in dogs. | staphylococcus pseudintermedius is a commensal organism of dogs that can also be implicated in surgical site infections (ssis) in dogs. particularly with the recent emergence and spread of the st71-t02-sccmecii-iii multidrug-resistant s. pseudintermedius clonal lineage (mdrsp), it is important to understand the clonal diversity of s. pseudintermedius in ssis in dogs. the study reported here investigated the genotypic relatedness of 124 s. pseudintermedius isolates from the surgical wounds of 90 ... | 2015 | 26449388 |
| methicillin-resistant staphylococcus pseudintermedius (mrsp) from healthy dogs in norway - occurrence, genotypes and comparison to clinical mrsp. | the aim of the study was to investigate the occurrence of methicillin-resistant staphylococcus pseudintermedius (mrsp) in healthy dogs and further to determine genetic relatedness between carrier isolates and clinical mrsp from dogs in norway. a total of 189 healthy dogs visiting ten veterinary clinics were screened for mrsp during the period february to april 2013. carrier isolates were susceptibility tested with disk diffusion and genotyped using multilocus sequence typing (mlst) and pulsed-fi ... | 2015 | 26423808 |
| association between methicillin-resistant staphylococcus pseudintermedius carriage and the development of surgical site infections following tibial plateau leveling osteotomy in dogs. | to evaluate the association between preoperative carriage of methicillin-resistant staphylococcus pseudintermedius (mrsp) and the development of surgical site infections (ssis) following tibial plateau leveling osteotomy (tplo) in dogs. | 2015 | 26421403 |
| negative pressure wound therapy with instillation for body wall reconstruction using an artificial mesh in a dachshund. | a 4-year-old spayed female dachshund was presented in shock, displaying multiple haematomas and puncture wounds along the left abdominal wall and ventral aspect of the abdomen after being attacked by another dog. a defect of the left lateral body wall was palpated. surgery revealed a massive body wall defect and concurrent injury of the intestines. surgical debridement was performed and the injured portion of the jejunum was resected. the abdominal wall was reconstructed using a polypropylene me ... | 2015 | 26412118 |
| impact of polymethylmethacrylate additives on methicillin-resistant staphylococcus pseudintermedius biofilm formation in vitro. | to evaluate the impact of gentamicin, silver, or both additives in polymethylmethacrylate (pmma) beads on methicillin-resistant staphylococcus pseudintermedius (mrsp) biofilm formation in vitro. | 2015 | 25909371 |
| phenotypic and genotypic characterization of canine pyoderma isolates of staphylococcus pseudintermedius for biofilm formation. | biofilm-forming ability is increasingly being recognized as an important virulence factor in several staphylococcus species. this study evaluated the biofilm-forming ability of sixty canine derived clinical isolates of s. pseudintermedius, using three phenotypic methods, microtiter plate test (mtp), congo red agar method (cra) and tube adherence test, and the presence and impact of biofilm-associated genes (icaa and icad). the results showed that icaa and icad genes were detected concomitantly i ... | 2015 | 25892615 |
| lincosamide resistance is less frequent in denmark in staphylococcus pseudintermedius from first-time canine superficial pyoderma compared with skin isolates from clinical samples with unknown clinical background. | antimicrobial resistance may be overestimated in bacterial isolates from clinical samples because veterinarians often submit samples in cases of treatment failure or recurrent cases, which are more frequently associated with resistant strains. | 2015 | 25891140 |
| a peptide factor secreted by staphylococcus pseudintermedius exhibits properties of both bacteriocins and virulence factors. | staphylococcus pseudintermedius is a common commensal bacterium colonizing the skin and mucosal surfaces of household animals. however, it has recently emerged as a dangerous opportunistic pathogen, comparable to s. aureus for humans. the epidemiological situation is further complicated by the increasing number of methicillin-resistant s. pseudintermedius infections and evidence of gene transmission driving antibiotic resistance between staphylococci colonizing human and zoonotic hosts. in the p ... | 2015 | 26411997 |
| emergence of biofilm-producing staphylococcus pseudintermedius isolated from healthy dogs in south korea. | staphylococcus pseudintermedius is a common bacterial pathogen in dogs with pyoderma, otitis externa, and wound infection. | 2015 | 26404519 |
| effectiveness of a combined (4% chlorhexidine digluconate shampoo and solution) protocol in mrs and non-mrs canine superficial pyoderma: a randomized, blinded, antibiotic-controlled study. | there is a lack of studies comparing topical antiseptics to systemic antibiotics in the treatment of canine superficial pyoderma. | 2015 | 26140535 |
| direct repeat unit (dru) typing of methicillin-resistant staphylococcus pseudintermedius from dogs and cats. | methicillin-resistant staphylococcus pseudintermedius (mrsp) has emerged in a remarkable manner as an important problem in dogs and cats. however, limited molecular epidemiological information is available. the aims of this study were to apply direct repeat unit (dru) typing in a large collection of well-characterized mrsp isolates and to use dru typing to analyze a collection of previously uncharacterized mrsp isolates. two collections of mrsp isolates from dogs and cats were included in this s ... | 2015 | 26378275 |
| new insights in staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain. | staphylococcus pseudintermedius is an opportunistic pathogen recognized as the leading cause of skin, ear, and post-operative bacterial infections in dogs and cats. zoonotic infections have also recently been reported causing endocarditis, infection of surgical wounds, rhinosinusitis, and catheter-related bacteremia. the aim of the present study is to evaluate, for the first time, the pathogenic potential of s. pseudintermedius isolated from a human infection. to this end, strain dsm 25713, whic ... | 2015 | 25994406 |
| isolation of coagulase-positive staphylococci from bitches' colostrum and milk and genetic typing of methicillin-resistant staphylococcus pseudintermedius strains. | among the coagulase-positive, potentially pathogenic staphylococci, staphylococcus pseudintermedius has been frequently isolated from bitches' milk. this organism colonizes the mammary gland or causes infection, while s. aureus has been only occasionally reported. the objective of this study was to investigate the occurrence and persistence of coagulase-positive staphylococci in the colostrum and milk of postpartum bitches, either treated or untreated with antimicrobials, and to assess the incid ... | 2015 | 26202520 |
| pharmacokinetics and pharmacokinetic/pharmacodynamic integration of marbofloxacin after intravenous and intramuscular administration in beagle dogs. | 1.the aim of the present study was to determine the pks of marbofloxacin in beagle dogs after intravenous (i.v.) and intramuscular (i.m.) administration, the ex vivo and in vitro pk/pd indices of marbofloxacin against clinical isolates of staphylococcus pseudintermedius, and the ex vivo auc/mic ratios associated with different levels of antibacterial activity. 2.after i.v. of marbofloxacin (2 mg/kg), the mean ± sem values of auc, t1/2β, vss, and cl were 8.47 ± 3.51 h µg/ml, 8.08 ± 6.25 h, 2.32 ± ... | 2015 | 25470431 |
| use of linezolid to treat mrsp bacteremia and discospondylitis in a dog. | a 1.5 yr old male german shepherd dog was evaluated for recurrent intermittent episodes of fever and lethargy. clinicopathologic abnormalities were suggestive of a discospondylitis at the seventh and eighth thoracic vertebrae. blood and urine cultures yielded growth of methicillin-resistant staphylococcus pseudintermedius (mrsp) that was resistant to all commonly used antibiotics. extralabel antibiotic susceptibility testing demonstrated susceptibility of both blood and urine isolates to linezol ... | 2015 | 24216492 |
| genomic insights into the rapid emergence and evolution of mdr in staphylococcus pseudintermedius. | mdr methicillin-resistant staphylococcus pseudintermedius (mrsp) strains have emerged rapidly as major canine pathogens and present serious treatment issues and concerns to public health due to their, albeit low, zoonotic potential. a further understanding of the genetics of resistance arising from a broadly susceptible background of s. pseudintermedius is needed. | 2015 | 25527273 |
| resistance to fluoroquinolones and methicillin in ophthalmic isolates of staphylococcus pseudintermedius from companion animals. | resistance to fluoroquinolones and methicillin was determined for 49 ophthalmic isolates of staphylococcus pseudintermedius from dogs with and without ophthalmic disease. resistance was observed for ciprofloxacin (40.8%), ofloxacin (38.8%), enrofloxacin (38.8%), levofloxacin (34.7%), and moxifloxacin (4.1%). eighteen isolates, 16 of which were resistant to oxacillin, were meca-positive. nine of the 16 oxacillin-resistant meca-positive s. pseudintermedius isolates were resistant to more than one ... | 2014 | 24982521 |
| antimicrobial susceptibility of methicillin-resistant staphylococcus pseudintermedius isolated from veterinary clinical cases in the uk. | staphylococcus pseudintermedius is a leading aetiologic agent of pyoderma and other body tissue infections in dogs and cats. in recent years, an increased prevalence of methicillin-resistant s. pseudintermedius (mrsp) has been reported. isolation of mrsp in serious infections poses a major therapeutic challenge as strains are often resistant to all forms of systemic antibiotic used to treat s. pseudintermedius -related infections. this study investigates the occurrence of mrsp from a total of 71 ... | 2014 | 24974679 |
| enhanced adherence of methicillin-resistant staphylococcus pseudintermedius sequence type 71 to canine and human corneocytes. | the recent worldwide spread of methicillin-resistant staphylococcus pseudintermedius (mrsp) in dogs is a reason for concern due to the typical multidrug resistance patterns displayed by some mrsp lineages such as sequence type (st) 71. the objective of this study was to compare the in vitro adherence properties between mrsp and methicillin-susceptible (mssp) strains. four mrsp, including a human and a canine strain belonging to st71 and two canine non-st71 strains, and three genetically unrelate ... | 2014 | 24957656 |
| in vitro synergism of fosfomycin and clarithromycin antimicrobials against methicillin-resistant staphylococcus pseudintermedius. | bacterial biofilms are of tremendous concern for clinicians, as they can compromise the ability of the immune system and antimicrobial therapy to resolve chronic and recurrent infections. novel antimicrobial therapies or combinations targeted against biofilm establishment and growth subsequently represent a promising new option for the treatment of chronic infectious diseases. in this study, we treated bacterial biofilms produced by methicillin-resistant staphylococcus pseudintermedius (mrsp) wi ... | 2014 | 24886369 |
| characterisation of clinical canine meticillin-resistant and meticillin-susceptible staphylococcus pseudintermedius in france. | staphylococcus pseudintermedius is a frequent pathogen in dogs. the emergence of meticillin-resistant s. pseudintermedius (mrsp), which is concomitantly resistant to nearly all veterinary licensed antibiotics used for systemic treatment in dogs, is a major problem for veterinarians. in france, 16.9% (41/243) of the s. pseudintermedius collected in 2010 were mrsp. they mainly belonged to the multiresistant mlst sequence type st71, spa type t02, sccmec type ii-iii (st71-t02-ii-iii) european clone. ... | 2014 | 27873590 |
| cluster of infections caused by methicillin-resistant staphylococcus pseudintermedius in humans in a tertiary hospital. | the dog-associated staphylococcus pseudintermedius is a rare pathogen in humans. here we describe a cluster of infections caused by the methicillin-resistant s. pseudintermedius clone st71-j-t02-ii-iii. it involved four elderly patients at a tertiary hospital. three patients had wound infections, and the strain had a tendency to cause bullous skin lesions. | 2014 | 24871217 |
| minocycline pharmacokinetics and pharmacodynamics in dogs: dosage recommendations for treatment of meticillin-resistant staphylococcus pseudintermedius infections. | although minocycline is not licensed for use in dogs, this tetracycline has therapeutic potential against meticillin-resistant staphylococcus pseudintermedius. | 2014 | 24840325 |
| staphylococcus pseudintermedius infection associated with nodular skin lesions and systemic inflammatory response syndrome in a dog. | a 10-year-old pekingese dog with atopic dermatitis was referred due to pyrexia, multiple skin nodules, anorexia, and depression. the dog was diagnosed as having systemic inflammatory response syndrome (sirs) induced by bacterial dermatitis. this case presents diagnosis and treatment of sirs with staphylococcal skin infection in a dog that was immunosuppressed due to long-term use of corticosteroid. | 2014 | 24790236 |
| guidelines for the diagnosis and antimicrobial therapy of canine superficial bacterial folliculitis (antimicrobial guidelines working group of the international society for companion animal infectious diseases). | superficial bacterial folliculitis (sbf) is usually caused by staphylococcus pseudintermedius and routinely treated with systemic antimicrobial agents. infection is a consequence of reduced immunity associated with alterations of the skin barrier and underlying diseases that may be difficult to diagnose and resolve; thus, sbf is frequently recurrent and repeated treatment is necessary. the emergence of multiresistant bacteria, particularly meticillin-resistant s. pseudintermedius (mrsp), has foc ... | 2014 | 24720433 |
| economic impact of tibial plateau leveling osteotomy surgical site infection in dogs. | to evaluate the economic impact of surgical site infection after tibial plateau leveling osteotomy (tplo) in dogs. | 2014 | 24617450 |
| antimicrobial resistance in methicillin susceptible and methicillin resistant staphylococcus pseudintermedius of canine origin: literature review from 1980 to 2013. | staphylococcus pseudintermedius is a commensal and a common opportunistic pathogen causing mainly infections of the integumentary system in dogs. the recent emergence of multidrug-resistant s. pseudintermedius isolates, in particular methicillin-resistant strains (mrsp) is a threat to small animal health and highlights the need for antimicrobial resistance surveillance to detect trends and potentially perform timeous interventions. we systematically reviewed 202 published articles to investigate ... | 2014 | 24613081 |
| fosfomycin susceptibility of canine methicillin-resistant staphylococcus pseudintermedius isolates. | the effectiveness of fosfomycin was examined across 31 methicillin-resistant staphylococcus pseudintermedius (mrsp) strains by agar dilution. prevalence of the fosfomycin-resistance determinant gene, fosb, was assessed by pcr analysis. results found that 84% of isolates were fosfomycin-susceptible. interestingly, 87% of isolates possessed fosb, indicating no association between this putative staphylococci resistance gene and phenotypic resistance. further evaluation of fosfomycin as a potential ... | 2014 | 24569298 |
| prevalence of mupirocin resistance in staphylococcus pseudintermedius. | in the united states, veterinary use of mupirocin is primarily limited to the treatment of canine pyoderma caused by methicillin-resistant staphylococcus pseudintermedius (mrsp). in this study, only 1 of 581 s. pseudintermedius isolates tested was resistant to mupirocin and carried the high-level mupirocin resistance gene, iles2, on a plasmid. | 2014 | 24478408 |
| case-control risk factor study of methicillin-resistant staphylococcus pseudintermedius (mrsp) infection in dogs and cats in germany. | methicillin-resistant staphylococcus pseudintermedius (mrsp) has emerged as a highly drug-resistant small animal veterinary pathogen. although often isolated from outpatients in veterinary clinics, there is concern that mrsp follows a veterinary-hospital-associated epidemiology. this study's objective was to identify risk factors for mrsp infections in dogs and cats in germany. clinical isolates of mrsp cases (n=150) and methicillin-susceptible s. pseudintermedius (mssp) controls (n=133) and the ... | 2014 | 24290489 |
| genetic relatedness, antimicrobial and biocide susceptibility comparative analysis of methicillin-resistant and -susceptible staphylococcus pseudintermedius from portugal. | forty methicillin-resistant and -susceptible staphylococcus pseudintermedius (mrsp and mssp, respectively) from colonization and infection in dogs and cats were characterized for clonality, antimicrobial, and biocide susceptibility. mssp were genetically more diverse than mrsp by multi-locus sequence typing and pulsed-field gel electrophoresis. three different spa types (t06, t02, t05) and two sccmec types (ii-iii and v) were detected in the mrsp isolates. all mrsp and two mssp strains were mult ... | 2014 | 23819785 |
| perioperative administration of antimicrobials during tibial plateau leveling osteotomy. | to evaluate perioperative antimicrobial administration during tibial plateau leveling osteotomy (tplo) in dogs at the ontario veterinary college health sciences centre. | 2014 | 25196889 |
| adverse events associated with chloramphenicol use in dogs: a retrospective study (2007-2013). | chloramphenicol is a broad spectrum antibiotic that has been increasingly utilised since the emergence of methicillin-resistant staphylococcal infections. due to toxicities in humans, use of the drug has been limited. in dogs, gastrointestinal signs are common adverse events described, and bone marrow suppression is possible. the aim of this study was to evaluate the adverse events associated with chloramphenicol in dogs seen by one specialty practice from january 2007 through june 2013. the dat ... | 2014 | 25096589 |
| large outbreak caused by methicillin resistant staphylococcus pseudintermedius st71 in a finnish veterinary teaching hospital--from outbreak control to outbreak prevention. | the purpose of this study was to describe a nosocomial outbreak caused by methicillin resistant staphylococcus pseudintermedius (mrsp) st71 sccmec ii-iii in dogs and cats at the veterinary teaching hospital of the university of helsinki in november 2010 - january 2012, and to determine the risk factors for acquiring mrsp. in addition, measures to control the outbreak and current policy for mrsp prevention are presented. | 2014 | 25333798 |
| antibacterial activity of novel cationic peptides against clinical isolates of multi-drug resistant staphylococcus pseudintermedius from infected dogs. | staphylococcus pseudintermedius is a major cause of skin and soft tissue infections in companion animals and has zoonotic potential. additionally, methicillin-resistant s. pseudintermedius (mrsp) has emerged with resistance to virtually all classes of antimicrobials. thus, novel treatment options with new modes of action are required. here, we investigated the antimicrobial activity of six synthetic short peptides against clinical isolates of methicillin-susceptible and mrsp isolated from infect ... | 2014 | 25551573 |
| characterization of meticillin-resistant and meticillin-susceptible isolates of staphylococcus pseudintermedius from cases of canine pyoderma in australia. | meticillin-resistant staphylococcus pseudintermedius (mrsp) has recently emerged as a worldwide cause of canine pyoderma. in this study, we characterized 22 s. pseudintermedius isolates cultured from 19 dogs with pyoderma that attended a veterinary dermatology referral clinic in australia in 2011 and 2012. twelve isolates were identified as mrsp by meca real-time pcr and phenotypic resistance to oxacillin. in addition to β-lactam resistance, mrsp isolates were resistant to erythromycin (91.6 %), ... | 2014 | 24994658 |
| identification of appropriate reference genes for qpcr studies in staphylococcus pseudintermedius and preliminary assessment of icaa gene expression in biofilm-embedded bacteria. | quantitative pcr is rapidly becoming the standard method for analyzing gene expression in a wide variety of biological samples however it can suffer from significant error if stably expressed reference genes are not identified on which to base the analysis. suitable reference genes for qpcr experiments on staphylococcus pseudintermedius have yet to be identified. | 2014 | 25023435 |
| design and application of a loop-mediated isothermal amplification assay for the rapid detection of staphylococcus pseudintermedius. | staphylococcus pseudintermedius is a commensal and opportunistic pathogen of dogs. it is mainly implicated in canine pyoderma, as well as other suppurative conditions of dogs. although bacterial culture is routinely used for clinical diagnosis, molecular methods are required to accurately identify and differentiate s. pseudintermedius from other members of the staphylococcus intermedius group. these methods, owing largely to their cost, are not easy to implement in nonspecialized laboratories or ... | 2014 | 24398904 |
| species differentiation within the staphylococcus intermedius group using a refined maldi-tof ms database. | among coagulase-positive staphylococci of animal origin, the members of the staphylococcus intermedius-group (sig: s. intermedius, staphylococcus pseudintermedius and staphylococcus delphini) are important opportunistic pathogens in different animal hosts and occasionally in humans. however, the unambiguous species diagnosis of sig is often challenging. therefore, matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) -based sig-identification with bruker mic ... | 2014 | 24807701 |
| a novel microfluidic wound model for testing antimicrobial agents against staphylococcus pseudintermedius biofilms. | current methods for testing treatments for veterinary surgical site infections can successfully emulate elements of a chronic wound, but these are time consuming and costly, requiring specialized laboratory equipment and considerable space to house study animals. microfluidic devices however, can be coated with collagen and maintained at basal body temperature, providing a more cost-effective and space-saving model of a chronic wound. our study assesses the applicability of a new microfluidic mo ... | 2014 | 24411017 |
| antibiotic resistance and molecular characterization of ophthalmic staphylococcus pseudintermedius isolates from dogs. | the prevalence, virulence potential, and antibiotic resistance of ophthalmic staphylococcus pseudintermedius (sp) isolated from dogs were examined. sixty-seven staphylococcus species were isolated from ophthalmic samples and surveyed for species-specific sequences in the staphylococcus intermedius group (sig) nuclease gene (sinuc), exfoliative toxin gene for sig (siet), and antibiotic resistance genes (blaz and meca). pcr-restriction fragment length polymorphism analysis of the pta gene was also ... | 2014 | 24690601 |
| first detection of methicillin-resistant staphylococcus aureus st398 and staphylococcus pseudintermedius st68 from hospitalized equines in spain. | eight coagulase-positive staphylococci from equines with different pathologies obtained between 2005 and 2011 were investigated. isolates were characterized by different molecular techniques (spa-, agr-, mlst), and clonal relatedness of strains was investigated by apai and smai pfge. anti-microbial resistance and virulence profiles were determined. six isolates were identified as staphylococcus aureus, and two as staphylococcus pseudintermedius. of these, four isolates were methicillin-resistant ... | 2014 | 23773775 |
| nasal carriage of methicillin-resistant staphylococcus aureus (mrsa) among swiss veterinary health care providers: detection of livestock- and healthcare-associated clones. | we screened a total of 340 veterinarians (including general practitioners, small animal practitioners, large animal practitioners, veterinarians working in different veterinary services or industry), and 29 veterinary assistants for nasal carriage of methicillin-resistant staphylococcus aureus (mrsa) and staphylococcus pseudintermedius (mrsp) at the 2012 swiss veterinary annual meeting. mrsa isolates (n = 14) were detected in 3.8 % (95 % ci 2.1 - 6.3 %) of the participants whereas mrsp was not d ... | 2014 | 24973319 |
| carriage of methicillin-resistant staphylococci by healthy companion animals in the us. | antimicrobial-resistant staphylococci have been associated with wounded or ill companion animals, but little is known about the prevalence of resistant staphylococci among healthy animals. in this study, 276 healthy dogs and cats from veterinary clinics were tested for the presence of antimicrobial-resistant staphylococcus spp. isolates were tested for antimicrobial susceptibility and the presence of select resistance genes, and typed using pulsed-field gel electrophoresis (pfge). staphylococcus ... | 2014 | 24730724 |
| staphylococcus pseudintermedius for camp-test. | camp test reliably detects listeria monocytogenes (lm) and streptococcus agalactiae (group b streptococcus, gbs); it is traditionally performed streaking the tested isolate perpendicularly to staphylococcus aureus (sa), provided that reference sa strains (that produce β-hemolysin) are used. in a zone of β-hemolysin activity, in fact, gbs and lm form typical arrow-shaped hemolytic areas. while sa production of the toxin is strain-dependent, however, that of staphylococcus pseudintermedius (sp), a ... | 2014 | 24817971 |
| strain typing and antimicrobial susceptibility of methicillin-resistant coagulase-positive staphylococcal species in dogs and people associated with dogs in thailand. | this study was to investigate and to characterize methicillin-resistant coagulase-positive staphylococci (mrcops) harboring in dogs and people associated with dogs in thailand. | 2014 | 24833550 |
| amikacin resistance in staphylococcus pseudintermedius isolated from dogs. | staphylococcus pseudintermedius is the most common microorganism isolated from canine pyoderma and postoperative wound infections. the prevalence of methicillin-resistant s. pseudintermedius (mrsp) has increased, and recently, isolates that are resistant not only to methicillin but also to other classes of antibiotic drugs, including aminoglycosides, have become common. a total of 422 s. pseudintermedius isolates collected from 413 dogs were analyzed for amikacin and methicillin resistance using ... | 2014 | 25078911 |
| prevalence and characterization of staphylococcus aureus and staphylococcus pseudintermedius isolated from companion animals and environment in the veterinary teaching hospital in zambia, africa. | the republic of zambia consists of only one veterinary teaching school at the university of zambia (unza) where students and veterinarians are exposed to many bacterial pathogens including staphylococcus aureus (sa) and staphylococcus pseudintermedius (sp). the aim of this study was the characterization and antimicrobial susceptibility profile of eleven sa and 48 sp isolates from the veterinary hospitals' in- and outpatients and the environment. no isolate was resistant to cefoxitin by disk diff ... | 2014 | 24480623 |
| small colony variant of methicillin-resistant staphylococcus pseudintermedius st71 presenting as a sticky phenotype. | we first observed the phenomenon of small colony variants (scvs) in a staphylococcus pseudintermedius sequence type 71 (st71) strain, isolated from a non-pet owner. although we found that small-sized colonies share main features with staphylococcus aureus scvs, they nevertheless show a novel, particular, and sticky phenotype, whose expression was extremely stable, even after subcultivation. | 2014 | 24452163 |
| in vitro evaluation of the use of diluted sodium hypochlorite (bleach) against staphylococcus pseudintermedius, pseudomonas aeruginosa and malassezia pachydermatis. | 2014 | 24589038 | |
| apo, zn2+-bound and mn2+-bound structures reveal ligand-binding properties of sita from the pathogen staphylococcus pseudintermedius. | the gram-positive bacterium staphylococcus pseudintermedius is a leading cause of canine bacterial pyoderma, resulting in worldwide morbidity in dogs. s. pseudintermedius also causes life-threatening human infections. furthermore, methicillin-resistant s. pseudintermedius is emerging, resembling the human health threat of methicillin-resistant staphylococcus aureus. therefore it is increasingly important to characterize targets for intervention strategies to counteract s. pseudintermedius infect ... | 2014 | 25311310 |
| diversity and evolution of oligopeptide permease systems in staphylococcal species. | several oligopeptide permease (opp) systems have been found in staphylococcal species, including opp1-4, opp3' and the arginine catabolic mobile element (acme)-encoded opp system (acme-opp). they confer upon bacteria the increasing fitness, but their evolutionary histories remain unclear. in this work, we performed a genome-wide identification of opp systems in staphylococcal species. novel opp systems were identified, including the duplicate of opp4 in staphylococcus pseudintermedius and the ac ... | 2014 | 24793159 |
| a case of methicillin-resistant staphylococcus pseudintermedius (mrsp) pyoderma in a labrador retriever dog. | an 8-year-old, neutered male labrador retriever dog with generalized pruritis had a history of recurring atopic dermatitis and superficial pyoderma. cocci and yeast were found on cytology and methicillin-resistant staphylococcus pseudintermedius was cultured. a regimen of marbofloxacin, dexamethasone, ketoconazole, and cyclosporine in addition to bathing with 2% chlorhexidine shampoo resulted in marked improvement. | 2014 | 25392557 |
| synergistic hemolysis between β-lysin-producing staphylococcus species and rothia nasimurium in primary cultures of clinical specimens obtained from dogs. | nonhemolytic isolates of catalase-positive, gram-positive cocci were observed that produced strong synergistic hemolysis with colonies of staphylococcus pseudintermedius and staphylococcus schleiferi subsp. coagulans in primary cultures of clinical specimens from dogs. the isolates appeared similar to nonhemolytic staphylococci but were not identified with conventional biochemical tests or commercial biochemical test kits. partial 16s ribosomal rna gene sequences from each of 9 tested isolates w ... | 2014 | 24760132 |
| inactivation of microorganisms by low-frequency high-power ultrasound: 1. effect of growth phase and capsule properties of the bacteria. | the aim of this study was to determine the effects of high-intensity low-frequency (20 khz) ultrasound treatment on the viability of bacteria suspension. more specifically, we have investigated the relationship between the deactivation efficiency and the physical (size, hydrophobicity) and biological (gram-status, growth phase) properties of the microbes. enterobacter aerogenes, bacillus subtilis, staphylococcus epidermidis, s. epidermidis sk and staphylococcus pseudintermedius were chosen for t ... | 2014 | 23835398 |
| characterisation of bacterial growth and antimicrobial susceptibility patterns in canine urinary tract infections. | bacterial urinary tract infection (uti) is a common reason for antimicrobial therapy in dogs. a reported increase in multi-drug resistance in canine bacterial pathogens, including resistance to extended-spectrum cephalosporins (esc) is of concern as antimicrobial resistance complicates therapy in dogs. in addition, it is a possible public health concern. the objectives of this study were to investigate the relative prevalence of pathogens in urine samples from dogs with urinary tract infection s ... | 2014 | 25249356 |
| canine antimicrobial peptides are effective against resistant bacteria and yeasts. | antimicrobial peptides (amps) are small proteins present in most living species and they are involved in the defence against pathogenic organisms. β-defensins and cathelicidin are the most frequently studied amps in both people and dogs. | 2014 | 24215268 |
| staphylococcus pseudintermedius and staphylococcus schleiferi subspecies coagulans from canine pyoderma cases in grenada, west indies, and their susceptibility to beta-lactam drugs. | over a 2-year period 66 cases of canine pyoderma in grenada, west indies, were examined by aerobic culture in order to ascertain the bacteria involved and their antimicrobial resistance patterns. of the 116 total bacterial isolates obtained, the majority belonged to gram-positive species, and the most common organism identified through biochemical and molecular methods was staphylococcus pseudintermedius. additionally, identification of a staphylococcus schleiferi subspecies coagulans isolate wa ... | 2014 | 24592351 |
| canine superficial bacterial folliculitis: current understanding of its etiology, diagnosis and treatment. | superficial bacterial folliculitis (sbf) is more common in the dog than other mammalian species. until recently, a successful outcome in cases of canine sbf was possible by administering a potentiated amoxicillin, a first generation cephalosporin or a potentiated sulfonamide. unfortunately, this predictable susceptibility has changed, because methicillin resistant staphylococcus pseudintermedius (mrsp) and staphylococcus aureus (mrsa) are becoming more prevalent in canine sbf cases. the increasi ... | 2014 | 24345778 |
| dam-to-offspring transmission and persistence of staphylococcus pseudintermedius clones within dog families. | staphylococcus pseudintermedius is an opportunistic pathogen that causes mainly skin infections in dogs. although vertical transmission of s. pseudintermedius strains from dam to offspring has been reported, the persistence of the dam's strains in offspring over long periods of time is virtually unknown. | 2014 | 24267321 |
| misuse of antimicrobials and selection of methicillin-resistant staphylococcus pseudintermedius strains in breeding kennels: genetic characterization of bacteria after a two-year interval. | methicillin-resistant staphylococcus pseudintermedius (mrsp) strains have been isolated from dogs with increasing frequency; prolonged or excessive use of antimicrobials is associated with the selection of mrsp, and misuse of antimicrobials is frequent in breeding kennels. this study was carried out in two breeding kennels (a and b) in which we had isolated mrsp in 2008: the aim was to assess colonization of previously positive bitches and of other bitches sharing the same environment and to ass ... | 2013 | 22551469 |
| residual antibacterial activity of dog hairs after therapy with antimicrobial shampoos. | topical antimicrobial treatment for canine pyoderma is becoming increasingly important, but little is known about the mechanism of action and persistence of activity of antimicrobial shampoos. | 2013 | 23432432 |
| usefulness of cefovecin disk-diffusion test for predicting meca gene-containing strains of staphylococcus pseudintermedius and clinical efficacy of cefovecin in dogs with superficial pyoderma. | cefovecin has been widely used to treat skin infections in dogs. the relationship of the cefovecin disk-diffusion test results to the presence of the meca gene and the clinical efficacy of cefovecin have not been fully evaluated. | 2013 | 23331693 |
| in vitro antiseptic susceptibilities for staphylococcus pseudintermedius isolated from canine superficial pyoderma in japan. | topical therapy, particularly with chlorhexidine, is becoming increasingly common as a treatment option for canine pyoderma; however, there are limited studies on the susceptibility of staphylococcus pseudintermedius to chlorhexidine compounds. | 2013 | 23331688 |
| serum anti-staphylococcus pseudintermedius ige and igg antibodies in dogs with atopic dermatitis and nonatopic dogs. | dogs and humans with atopic dermatitis (ad) are predisposed to colonization and recurrent infection with staphylococcus spp. studies in humans suggest that staphylococcus-specific immunoglobulin e (ige) plays a key role in disease pathogenesis. few such studies have been undertaken in dogs. | 2013 | 23331675 |
| pathogenicity potential of staphylococcus pseudintermedius strains isolated from canine carriers and from dogs with infection signs. | 2013 | 23328490 | |
| methicillin-resistant staphylococcus pseudintermedius in rats. | 2013 | 23260061 | |
| feasibility of magnetic resonance electrical impedance tomography (mreit) conductivity imaging to evaluate brain abscess lesion: in vivo canine model. | to show the feasibility of magnetic resonance electrical impedance tomography (mreit) conductivity imaging in terms of its capability to provide new contrast information of abscess lesion and characterize time-course variations before and after the induction of brain abscess. | 2013 | 23239506 |
| multilocus sequence typing for characterization of staphylococcus pseudintermedius. | staphylococcus pseudintermedius is an opportunistic pathogen in dogs. four housekeeping genes with allelic polymorphisms were identified and used to develop an expanded multilocus sequence typing (mlst) scheme. the new seven-locus technique shows s. pseudintermedius to have greater genetic diversity than previous methods and discriminates more isolates based upon host origin. | 2013 | 23115265 |
| evaluation of minocycline susceptibility of methicillin-resistant staphylococcus pseudintermedius. | methicillin-resistant staphylococcus pseudintermedius (mrsp) is an increasingly important cause of opportunistic infections in dogs and cats. treatment options are often limited because of the high level of antimicrobial resistance. doxycycline resistance is common, but variable mechanisms of doxycycline resistance exist, with some conferring resistance to doxycycline but not minocycline. however, there has been limited study of minocycline susceptibility in clinical mrsp isolates nor has the as ... | 2013 | 23107659 |
| transcription of canine toll-like receptor 2, β-defensin 1 and β-defensin 103 in infected atopic skin, non-infected atopic skin, healthy skin and the cpek cell line. | dogs and humans with atopic dermatitis (ad) have a high prevalence of recurrent staphylococcal pyoderma. β-defensins (bds) and toll-like receptors (tlrs) are important in innate immunity against bacterial skin infections, and decreased bd and tlr2 expression has been associated with human ad. however, findings from recent studies of bd expression in human and canine ad have been variable and contradictory. the aim of this study was to further our understanding of the role of antimicrobial protei ... | 2013 | 23067725 |
| in vitro evaluation of dispersinb on methicillin-resistant staphylococcus pseudintermedius biofilm. | methicillin resistant staphylococcus pseudintermedius (mrsp) is an important canine pathogen that has been shown to produce biofilm in vitro. biofilm production may be an important virulence factor and methods to eliminate biofilm-associated infections are required. an enzyme of aggregatibacter actinomycetemcomitans, dispersinb, has been shown to degrade the extracellular matrix in the biofilm of a variety of bacteria including staphylococcus aureus and staphylococcus epidermidis. the objective ... | 2013 | 23932310 |
| susceptibility of canine and feline bacterial pathogens to pradofloxacin and comparison with other fluoroquinolones approved for companion animals. | in this study, 908 bacterial pathogens from defined infections of dogs and cats were tested for their susceptibility to the novel fluoroquinolone pradofloxacin, which was approved in 2011 for use in cats and dogs. most of the bacteria tested (staphylococcus aureus, staphylococcus pseudintermedius, escherichia coli, β-haemolytic streptococci, pasteurella multocida and bordetella bronchiseptica) exhibited low pradofloxacin mic(90) values of ≤ 0.25 μg/ml. solely proteus spp. and pseudomonas aerugin ... | 2013 | 22939523 |
| an investigation of resistance to β-lactam antimicrobials among staphylococci isolated from pigs with exudative epidermitis. | a high proportion of staphylococci isolated from pigs affected with exudative epidermitis were found to be resistant to β-lactam antimicrobials. the primary objective of this research was to investigate and characterize β-lactam resistance in staphylococcus hyicus, staphylococcus aureus and other staphylococci isolated from these pigs. | 2013 | 24131819 |
| antibiotic treatment of resistant infections in small animals. | there are few veterinary clinical studies to support a recommended use and dose for treating resistant bacterial infections in small animals. resistance against many common antibiotics is possible and a susceptibility test is advised. infections caused by pseudomonas aeruginosa presents a special problem. staphylococcus isolated from small animals is most likely to be staphylococcus pseudintermedius. the most important resistance mechanism for staphylococcus is methicillin resistance. the only a ... | 2013 | 23890241 |
| in vitro comparison of the effectiveness of polihexanide and chlorhexidine against canine isolates of staphylococcus pseudintermedius, pseudomonas aeruginosa and malassezia pachydermatis. | polihexanide (polyhexamethylene biguanide) is an antiseptic substance that plays a prominent role in the treatment of critically colonized or infected acute and chronic wounds in humans. | 2013 | 23789723 |
| the joint in vitro action of polymyxin b and miconazole against pathogens associated with canine otitis externa from three european countries. | canine otitis externa, an inflammation of the external ear canal, can be maintained and worsened by bacterial or fungal infections. for topical treatment, combinations of anti-inflammatory and antimicrobial ingredients are mainly used. | 2013 | 23721182 |
| mutant prevention concentration of orbifloxacin: comparison between escherichia coli, pseudomonas aeruginosa, and staphylococcus pseudintermedius of canine origin. | the mutant prevention concentration (mpc) is an important parameter to evaluate the likelihood of growth of fluoroquinolone-resistant mutants for antimicrobial-pathogen combinations. the mpcs of fluoroquinolones for different canine pathogens have not been compared. in this study, we compared for the first time orbifloxacin mpcs between susceptible strains of escherichia coli, pseudomonas aeruginosa, and staphylococcus pseudintermedius of canine origin. | 2013 | 23634902 |
| characterisation of a catalase-negative methicillin-resistant staphylococcus aureus isolate from a dog. | a catalase-negative mrsa strain and a methicillin resistant staphylococcus pseudintermedius strain (mrsp) were isolated from a dog affected by a severe form of pododermatitis. the catalase negative isolate was typed as sccmec i, pvl negative, st5 t002 strain. a deletion at position 487 of the kat gene altered the functionality of the catalase enzyme. this is the first report of a catalase-negative mrsa in animals. as catalase test is a rapid assay routinely employed for the identification of sta ... | 2013 | 24139722 |
| high diversity of staphylococcus aureus and staphylococcus pseudintermedius lineages and toxigenic traits in healthy pet-owning household members. underestimating normal household contact? | forty-three unrelated pet-owning households were screened in spain to study the staphylococcus aureus and staphylococcus pseudintermedius nasal carriage, their genetic lineages and virulence traits. sixty-seven healthy owners and 66 healthy pets were investigated. isolates characterization was performed and potential interspecies transmission was assessed. s. aureus was present in 51.2% of households studied while s. pseudintermedius in 30.2%. twenty-eight owners (41.8%) carried s. aureus: one m ... | 2013 | 23153600 |
| public health impact and antimicrobial selection of meticillin-resistant staphylococci in animals. | whilst meticillin-resistant staphylococcus aureus (mrsa) infections reported sporadically in companion animals and cattle in the 1970s were probably of human origin, the recently emerged livestock-associated mrsa (la-mrsa) and meticillin-resistant staphylococcus pseudintermedius (mrsp) strains clearly have animal origins and their isolation from humans is usually associated with exposure to livestock and companion animals, respectively. la-mrsa is primarily an occupational health risk to farm wo ... | 2013 | 27873579 |
| a comparative evaluation of methicillin-resistant staphylococci isolated from harness racing-horses, breeding mares and riding-horses in italy. | to investigate the prevalence of methicillin-resistant staphylococci (mrs) which is a potencial risk factor of transmission between animals and humans in different types of horses (harness racing-horses, breeding mares and riding-horses) and to compare the antimicrobial resistance of the isolates. | 2013 | 23620832 |
| methicillin-resistant staphylococcal contamination of clothing worn by personnel in a veterinary teaching hospital. | to determine the methicillin-resistant staphylococcus aureus (mrsa) and methicillin-resistant staphylococcus pseudintermedius (mrsp) contamination rate of white coats (wc) and surgical scrubs (ss) worn by personnel at the ontario veterinary college health sciences centre (ovchsc) and to identify risk factors associated with clothing contamination. | 2013 | 23662728 |
| evidence for production of a bacteriocin-like substance by staphylococcus pseudintermedius, inhibitory to staphylococcus aureus from foods. | this study assessed the production of a bacteriocin-like substance by staphylococcus pseudintermedius s28, and evaluates its inhibitory effect against isolates of s. aureus from foods. all indicator isolates were sensitive to the substance produced from s. pseudintermedius s28, showing growth inhibition zones ranging from 14.2 to 28.3 mm. the inhibitory substance has no effect against the producer strain. the inhibitory substance was affected by proteolytic enzymes, while glycolytic and lipolyti ... | 2013 | 22703567 |
| evaluation of the affinity of various species and strains of staphylococcus to adhere to equine corneocytes. | meticillin-resistant staphylococcus aureus (mrsa) strain usa 500 predominately colonizes horses and people working with them. previous studies demonstrate that some staphylococcus species exhibit higher affinity for corneocytes of specific mammalian species. | 2013 | 23992283 |
| first report in south america of companion animal colonization by the usa1100 clone of community-acquired meticillin-resistant staphylococcus aureus (st30) and by the european clone of methicillin-resistant staphylococcus pseudintermedius (st71). | methicillin-resistant staphylococci can colonize and cause diseases in companion animals. unfortunately, few molecular studies have been carried out in brazil and other countries with the aim of characterizing these isolates. consequently, little is known about the potential role of companion animals in transmitting these resistant bacteria to humans. in this work we searched for meca gene among staphylococcus isolates obtained from nasal microbiota of 130 healthy dogs and cats attended in a vet ... | 2013 | 23981343 |
| animal and human staphylococcus aureus associated clonal lineages and high rate of staphylococcus pseudintermedius novel lineages in spanish kennel dogs: predominance of s. aureus st398. | methicillin-susceptible staphylococcus aureus (mssa) and staphylococcus pseudintermedius (mssp) are gaining interest to track the evolution of emerging methicillin-resistant strains in animals and humans. we focused on the characterization of the methicillin-susceptible coagulase-positive staphylococci (mscops) recovered from nasal samples of 98 healthy kennel-dogs. isolates were typed by spa, agr, mlst and smai/apai-pfge. antimicrobial resistance and virulence profiles were investigated. presen ... | 2013 | 23932077 |
| clonal dynamics of nasal staphylococcus aureus and staphylococcus pseudintermedius in dog-owning household members. detection of mssa st(398). | the objective of this study was to investigate the dynamics of nasal carriage by staphylococcus aureus (sa) and staphylococcus pseudintermedius (sp) among healthy dog-owning household members involved in 7 previous index cases of suspected anthropozoonotic (n = 4) and zoonotic (n = 3) interspecies transmission [4 direct cases, identical sa (n = 3) or sp (n = 1) in owner and dog; three indirect, sp in owner (n = 2) or sa in dog (n = 1)]. co-carriage with methicillin-resistant coagulase-negative s ... | 2013 | 23874949 |
| detection of mecc-positive staphylococcus aureus (cc130-mrsa-xi) in diseased european hedgehogs (erinaceus europaeus) in sweden. | recently, a novel mec gene conferring beta-lactam resistance in staphylococcus aureus has been discovered. this gene, mecc, is situated on a sccmec xi element that has to date been identified in clonal complexes 49, 130, 425, 599 and 1943. some of the currently known isolates have been identified from animals. this, and observations of meca alleles that do not confer beta-lactam resistance, indicate that mec genes might have a reservoir in staphylococcus species from animals. thus it is importan ... | 2013 | 23776626 |