Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| determination of minimum biofilm eradication concentrations of orbifloxacin for canine bacterial uropathogens over different treatment periods. | biofilm formation can cause refractory urinary tract infections (utis) in dogs; however, minimum biofilm eradication concentrations (mbecs) of veterinary drugs against canine uropathogens remain to be investigated. in this study, the mbecs of orbifloxacin (obfx), trimethoprim-sulfamethoxazole (tms) and amoxicillin/clavulanate (acv) over different time periods for treatment of canine uropathogenic escherichia coli (n = 10) were determined. the mbecs of obfx for other bacterial uropathogens, inclu ... | 2017 | 28042660 |
| multidrug-resistant opportunistic pathogens challenging veterinary infection control. | although the problems associated with healthcare-associated infections (hai) and the emergence of zoonotic and multidrug-resistant pathogens in companion animal (dogs, cats and horses) medicine have been well-known for decades, current progress with respect to practical implementation of infection control programs in veterinary clinics has been limited. clinical outbreak events reported for methicillin-resistant staphylooccus aureus (mrsa) and staphylococcus pseudintermedius (mrsp), extended spe ... | 2017 | 27291944 |
| presence of environmental coagulase-positive staphylococci, their clonal relationship, resistance factors and ability to form biofilm. | coagulase-positive staphylococci (cops) are opportunistic pathogens carrying various mechanisms of resistance that have a large number of virulence factors, and whose ability to induce illness is associated with the host. this study aimed to investigate the presence of environmental coagulase-positive staphylococci, their susceptibility profile, clonal relationship and ability to form biofilm. the 16s rrna genes from cops isolates were analyzed, and their antibiotic susceptibility was evaluated ... | 2017 | 28017482 |
| nasal carriage of coagulase positive staphylococci in patients of a primary-healthcare-center: genetic lineages and resistance and virulence genes. | staphylococcus aureus and staphylococcus pseudintermedius are highly important due to their capacity for producing diseases in humans and animals, respectively. the aim of the study was to investigate and characterize the coagulase positive staphylococcus (cops) carriage in a primary healthcare center population. | 2017 | 25459195 |
| antimicrobial resistance in staphylococcus pseudintermedius and the molecular epidemiology of methicillin-resistant s. pseudintermedius in small animals in finland. | 2017 | 28333277 | |
| complete genome sequence of staphylococcus pseudintermedius type strain lmg 22219. | we report the first complete genome sequence of lmg 22219 (=on 86(t) = ccug 49543(t)), the staphylococcus pseudintermedius type strain isolated from feline lung tissue. this sequence information will facilitate phylogenetic comparisons of staphylococcal species and other bacteria at the genome level. | 2017 | 28209834 |
| pyogenic ventriculitis and ventricular empyema associated with staphylococcus pseudintermedius in a puppy. | a 40-day-old male, blue heeler puppy with hindlimb ataxia, nystagmus, apathy, motor incoordination and hyperaesthesia of the forelimbs died 3 days after the onset of clinical signs. significant gross findings included cerebellar herniation, cerebral oedema and dilation of the third and right lateral cerebral ventricles due to the accumulation of a purulent exudate. histopathological examination revealed pyogenic ventriculitis and purulent meningoencephalitis. pure colonies of a coagulase-positiv ... | 2017 | 28041618 |
| alterations in conjunctival bacteria and antimicrobial susceptibility during topical administration of ofloxacin after cataract surgery in dogs. | objective to evaluate changes in conjunctival bacteria and antimicrobial susceptibility of bacteria after cataract surgery in dogs. animals 16 client-owned dogs. procedures samples for aerobic and anaerobic culture were obtained from the conjunctival fornices of both eyes of dogs 24 hours before (week 0) and 1, 3, and 6 weeks after cataract surgery. topical administration of ofloxacin (every 6 hours) was initiated 12 hours before surgery and continued for 3 weeks. in vitro antimicrobial suscepti ... | 2017 | 28140645 |
| establishing a canine superficial pyoderma model. | pyoderma, predominantly associated with staphylococcus pseudintermedius, is a common skin infection of dogs that typically requires long-lasting treatments, complicated by increasing antimicrobial resistance. to investigate new treatment strategies, we aimed at establishing a dog model of pyoderma that closely mimics the natural disease. | 2017 | 27891724 |
| diagnostic microbiology in veterinary dermatology: present and future. | the microbiology laboratory can be perceived as a service provider rather than an integral part of the healthcare team. | 2017 | 28133869 |
| staphylococcus pseudintermedius human infection cases in spain: dog-to-human transmission. | staphylococcus pseudintermedius is an opportunistic pathogen that has been identified as infectious agent or colonizer mainly in dogs. s. pseudintermedius has been also detected in humans and more specifically in people in contact with dogs. in this study, the possible s. pseudintermedius pet-to-human transmission was analyzed in four clinical human cases. two patients were dog owners and s. pseudintermedius was also detected as colonizer in these healthy animals. s. pseudintermedius isolates fr ... | 2017 | 28075235 |
| antimicrobial resistance in staphylococcus pseudintermedius and the molecular epidemiology of methicillin-resistant s. pseudintermedius in small animals in finland. | to investigate antimicrobial susceptibility in staphylococcus pseudintermedius and the occurrence of methicillin-resistant s. pseudintermedius (mrsp), to explore the molecular structure of the mrsp population and to analyse risk factors for mrsp. | 2017 | 28065889 |
| nasal carriage of methicillin-resistant staphylococcus pseudintermedius in dogs treated with cephalexin monohydrate. | this study aimed to investigate the nasal carriage of methicillin-resistant staphylococcus pseudintermedius (mrsp) in dogs treated with oral cephalexin monohydrate. ten dogs with superficial pyoderma were monitored longitudinally for carriage of mrsp for up to 1 year after treatment; the strains were typed and antibiograms were determined. methicillin-susceptible s. pseudintermedius (mssp) was recovered prior to treatment in all dogs and could be isolated after 12 months in 1 dog. methicillin-re ... | 2017 | 28042159 |
| clinical, microscopic and microbial characterization of exfoliative superficial pyoderma-associated epidermal collarettes in dogs. | the microscopic and microbial features of the spreading epidermal collarettes of canine exfoliative superficial pyodermas are poorly characterized. | 2017 | 27426474 |
| public health risk of antimicrobial resistance transfer from companion animals. | antimicrobials are important tools for the therapy of infectious bacterial diseases in companion animals. loss of efficacy of antimicrobial substances can seriously compromise animal health and welfare. a need for the development of new antimicrobials for the therapy of multiresistant infections, particularly those caused by gram-negative bacteria, has been acknowledged in human medicine and a future corresponding need in veterinary medicine is expected. a unique aspect related to antimicrobial ... | 2017 | 27999066 |
| the in vitro antibacterial activity of incomplete iron salt of polyacrylic acid against pseudomonas aeruginosa, meticillin-resistant staphylococcus pseudintermedius and meticillin-resistant s. aureus. | the incomplete iron salt of polyacrylic acid (iis-paa) has been used for its haemostatic, wound healing and antibacterial properties in human patients. iis-paa is a water-soluble, acidic, hydrophilic polymer that is active at ph 2.9-4.0. | 2017 | 28833656 |
| bacterial recovery using sonication versus swabbing of titanium and stainless steel implants inoculated with staphylococcus pseudintermedius or pseudomonas aeruginosa. | to evaluate the use of sonication to improve recovery of bacteria from metal discs infected with bacteria commonly associated with implant infections in veterinary medicine. | 2017 | 28763526 |
| a spinal infection with staphylococcus pseudintermedius. | we present a case of a 60-year-old woman with an invasive spinal infection with staphylococcus pseudintermedius associated with a 15-year-old spinal fixation device and epidemiological contact with dogs. it was confirmed on blood culture and culture from pus from the epidural abscess and successfully treated using similar treatment as for a staphylococcus aureus infection - 6 weeks of intravenous flucloxacillin 2 g four times daily with a 6 week follow-on course of oral clindamycin 450 mg three ... | 2017 | 28784907 |
| prevalence of methicillin-resistant staphylococcus aureus from equine nasopharyngeal and guttural pouch wash samples. | methicillin-resistant staphylococcus aureus (mrsa) is recognized as a cause of nosocomial infections in both human and veterinary medicine. studies that examine the nasopharynx and guttural pouches of the horse as carriage sites for mrsa have not been reported. | 2017 | 28661019 |
| in vitro comparison of the dermal penetration of three different topical formulations containing lasalocid. | topical antimicrobial preparations are of utmost importance in treating suspected and confirmed meticillin-resistant staphylococcus pseudintermedius (mrsp) infections due to the increasing incidence of widespread resistance to systemic antimicrobials. lasalocid is active against mrsp in vitro and this may become an important topical antimicrobial for the treatment of canine pyoderma. | 2017 | 28470877 |
| survival of staphylococcus pseudintermedius in modified romanowsky staining solutions. | stains that are used regularly for patient-side diagnosis to rapidly identify bacterial and fungal infections could become contaminated by common pathogens, such as staphylococcus pseudintermedius, during slide immersion. | 2017 | 28470871 |
| antimicrobial resistance in coagulase-positive staphylococci isolated from companion animals in australia: a one year study. | methicillin-resistant coagulase-positive staphylococci (cops) have become increasingly recognised as opportunistic pathogens that limit therapeutic options in companion animals. the frequency of methicillin resistance amongst clinical isolates on an australia-wide level is unknown. this study determined antimicrobial susceptibility patterns for cops isolated from clinical infections in companion animals (dogs, cats and horses) as part of the first nation-wide survey on antimicrobial resistance i ... | 2017 | 28430811 |
| in vitro bactericidal activity of blue light (465 nm) phototherapy on meticillin-susceptible and meticillin-resistant staphylococcus pseudintermedius. | staphylococcus pseudintermedius is the most common cause of bacterial skin infections in dogs. meticillin-resistant infections have become more common and are challenging to treat. blue light phototherapy may be an option for treating these infections. | 2017 | 28543810 |
| patterns and predictors of antimicrobial resistance among staphylococcus spp. from canine clinical cases presented at a veterinary academic hospital in south africa. | antimicrobial resistance in staphylococci, often associated with treatment failure, is increasingly reported in veterinary medicine. the aim of this study was to investigate patterns and predictors of antimicrobial resistance among staphylococcus spp. isolates from canine samples submitted to the bacteriology laboratory at the university of pretoria academic veterinary hospital between 2007 and 2012. retrospective data of 334 staphylococcus isolates were used to calculate the proportion of sampl ... | 2017 | 28454567 |
| development of a real-time pcr for detection of staphylococcus pseudintermedius using a novel automated comparison of whole-genome sequences. | staphylococcus pseudintermedius is an opportunistic pathogen in dogs and cats and occasionally causes infections in humans. s. pseudintermedius is often resistant to multiple classes of antimicrobials. it requires a reliable detection so that it is not misidentified as s. aureus. phenotypic and currently-used molecular-based diagnostic assays lack specificity or are labour-intensive using multiplex pcr or nucleic acid sequencing. the aim of this study was to identify a specific target for real-t ... | 2017 | 28859126 |
| taurolidine susceptibility of methicillin-resistant staphylococcus pseudintermedius isolates. | infections caused by methicillin-resistant staphylococcus pseudintermedius (mrsp) can be challenging to treat because they are often resistant to many other antimicrobial agents. we assessed the susceptibility of 29 mrsp isolates from dogs to taurolidine in vitro. there was no growth at 0.12% taurolidine and light growth at 0.06% for all isolates. taurolidine was reliable at inhibiting growth of mrsp at a concentration of 1,200 µg/ml. | 2017 | 28754087 |
| direct repeat unit (dru) typing and antimicrobial resistance of methicillin-resistant staphylococcus pseudintermedius isolated from dogs in atlantic canada. | there are few reports investigating the characterization of methicillin-resistant staphylococcus pseudintermedius (mrsp) in dogs in canada and none from atlantic canada. the objectives of this study were to strain type mrsp isolates cultured at a regional diagnostic laboratory using direct repeat unit (dru) typing and to describe their antimicrobial resistance profiles. ninety-four isolates recovered from dogs between 2010 and 2012 had dru typing, cluster analysis, and antimicrobial susceptibili ... | 2017 | 28725109 |
| molecular characteristics of clinical methicillin-resistant staphylococcus pseudintermedius harboring arginine catabolic mobile element (acme) from dogs and cats. | this study aimed to investigate the presence of arginine catabolic mobile element (acme) and its associated molecular characteristics in methicillin-resistant staphylococcus pseudintermedius (mrsp). among the 72 s. pseudintermedius recovered from various infection sites of dogs and cats, 52 (72.2%) were mrsp. acme-arca was detected commonly (69.2%) in these mrsp isolates, and was more frequently detected in those from the skin than from other body sites (p=0.047). there was a wide genetic divers ... | 2017 | 28697875 |
| high-resolution melting analysis of gyra codon 84 and grla codon 80 mutations conferring resistance to fluoroquinolones in staphylococcus pseudintermedius isolates from canine clinical samples. | staphylococcus pseudintermedius is an opportunistic pathogen of dogs and cats. a high-resolution melting analysis (hrma) protocol was designed and tested on 42 clinical isolates with known fluoroquinolone (fq) susceptibility and gyra codon 84 and grla codon 80 mutation status. the hrma approach was able to discriminate between fq-sensitive and fq-resistant strains and confirmed previous reports that the main mutation site associated with fq resistance in s. pseudintermedius is located at positio ... | 2017 | 28578596 |
| clonal complexes and antimicrobial susceptibility profiles of staphylococcus pseudintermedius isolates from dogs in the united states. | staphylococcus pseudintermedius is the primary cause of canine pyoderma and has been associated with diseases in other animals, including human beings. a high prevalence of methicillin and multidrug resistance has been reported in this bacterium in some geographic regions of the united states. multilocus sequence type (mlst) 68 was implicated, initially, as the major clonal genotype based on a limited number of samples. the objectives of this study were to determine the population genetics of s. ... | 2017 | 28504897 |
| validity of somatic cell count as indicator of pathogen-specific intramammary infections. | the objective of this study was to determine whether somatic cell count (scc) was an effective test, with a sensitivity exceeding 85%, to determine species-specific bacterial infections. in addition, the relation between the scc and various udder pathogen groups was investigated. scc thresholds of greater than 200 000 cells/ml were used in quarter and greater than 150 000 cells/ml in composite milk samples. a retrospective study was conducted on a data set for 89 635 quarter and 345 467 composit ... | 2017 | 28470079 |
| skin infection caused by a novel strain of staphylococcus pseudintermedius in a siberian husky dog owner. | introduction.staphylococcus pseudintermedius, an opportunistic pathogen of dogs and cats, is rarely reported to cause infection in humans. here, we describe a case of severe skin infection caused by s. pseudintermedius, in a 47-year-old male, a dog owner; to the best of our knowledge, this is the first such case reported from scotland. case presentation. the patient presented with a short history of a severe ecthyma-like lesion on his forehead, with smaller lesions on his abdomen and legs. bacte ... | 2017 | 28663821 |
| quantification and characterization of pleural fluid in healthy dogs with thoracostomy tubes. | objective to quantify and characterize pleural fluid collected from healthy dogs after placement of a thoracostomy tube (tt). animals 8 healthy coonhound-cross dogs (mean ± sd weight, 27.2 ± 1.6 kg). procedures thoracic ct of each dog was performed before placement of a tt and daily thereafter for 7 days. thoracic fluid volume was calculated from ct images. effusion was aspirated when detected; volume was recorded, and cytologic analysis and bacterial culture were performed. results mean ± sd vo ... | 2016 | 27901387 |
| new categories designated as healthcare-associated and community-associated methicillin-resistant staphylococcus pseudintermedius in dogs. | the incidence of mrsp has been increasing, and treatment options in veterinary medicine are limited. few previous studies of mrsp have described the relationships between the genotypes, phenotypes, and clinical backgrounds of the isolates. to gain insight into the associations between the microbiological and clinical characteristics of mrsp, we analyzed 282 staphylococcus pseudintermedius isolates from dogs. a total of 195 (69.1%) strains were identified as meca-positive mrsp and were classified ... | 2016 | 27417508 |
| interaction of chlorhexidine with trisedta or miconazole in vitro against canine meticillin-resistant and -susceptible staphylococcus pseudintermedius isolates from two uk regions. | topical therapy is an important alternative to systemic antibacterial therapy for treatment of canine superficial pyoderma in light of the emergence of multidrug-resistant staphylococci. chlorhexidine is widely used in shampoo products alone or in combination with miconazole or tromethamine-ethylenediaminetetraacetic acid (trisedta). comparisons of these combinations have not been made. | 2016 | 27406860 |
| residual antibacterial activity of canine hair treated with topical antimicrobial sprays against staphylococcus pseudintermedius in vitro. | topical antimicrobial therapy is increasingly important in the treatment of canine pyoderma as the incidence of multidrug resistance has risen. however, little information is reported on the persistence of activity of topical antimicrobial products. | 2016 | 27374468 |
| high genotypic diversity among methicillin-resistant staphylococcus pseudintermedius isolated from canine infections in denmark. | methicillin-resistant staphylococcus pseudintermedius (mrsp) has emerged globally in companion animals in the last decade. in europe, the multidrug-resistant sequence type (st)71 is widespread, but recently other clones have appeared. the objective of this study was to examine genotypic diversity and antimicrobial resistance of clinical mrsp isolates obtained from dogs, including dogs sampled on multiple occasions, in denmark over a six-year period. for that purpose a total of 46 clinical mrsp i ... | 2016 | 27357502 |
| antimicrobial synergy between carprofen and doxycycline against methicillin-resistant staphylococcus pseudintermedius st71. | new therapeutic strategies are needed to face the rapid spread of multidrug-resistant staphylococci in veterinary medicine. the objective of this study was to identify synergies between antimicrobial and non-antimicrobial drugs commonly used in companion animals as a possible strategy to restore antimicrobial susceptibility in methicillin-resistant staphylococcus pseudintermedius (mrsp). | 2016 | 27342694 |
| acquisition of the fexa and cfr genes in staphylococcus pseudintermedius during florfenicol treatment of canine pyoderma. | 2016 | 27750159 | |
| characterization of canine and feline methicillin-resistant staphylococcus pseudintermedius (mrsp) from thailand. | methicillin-resistant staphylococcus pseudintermedius (mrsp) in small animal practice are very difficult to treat due to multi-resistance. in contrast to other countries, little is known about mrsp from thailand. in particular, information on feline mrsp isolates in general is rare. in total, 39 mrsp isolates from dogs (n=28) and cats (n=11) from thailand collected from independent clinical cases were used. oxacillin resistance and presence of the meca gene was confirmed. susceptibility to addit ... | 2016 | 27160358 |
| evaluation of a silver-impregnated coating to inhibit colonization of orthopaedic implants by biofilm forming methicillin-resistant staphylococcus pseudintermedius. | to evaluate the in vitro antibacterial activity of a silver-impregnated coating against a biofilm-forming strain of methicillin-resistant staphylococcus pseudintermedius (mrsp). | 2016 | 27102292 |
| treatment of a perforating thoracic bite wound in a dog with negative pressure wound therapy. | case description a 4-year-old male dachshund was examined following a bite attack that had occurred 5 days previously. the dog had acutely deteriorated despite iv antimicrobial treatment and fluid therapy. clinical findings on initial examination, the patient was recumbent with signs of septic shock and a flail chest. three penetrating wounds in the left thoracic wall with malodorous discharge were evident. the animal trauma triage score was 8 out of 18. thoracic and abdominal radiography reveal ... | 2016 | 27654166 |
| evaluation of the impact of methicillin-resistant staphylococcus pseudintermedius biofilm formation on antimicrobial susceptibility. | to compare the minimum inhibitory concentration (mic) of four antimicrobials in planktonic vs. biofilm-associated staphylococcus pseudintermedius. | 2016 | 27500604 |
| the skin microbiome in allergen-induced canine atopic dermatitis. | studies focusing on next-generation sequencing of the bacterial 16s rrna gene have allowed detailed surveys of skin bacterial populations (microbiota) of the skin. | 2016 | 27485242 |
| resistance of canine methicillin-resistant staphylococcus pseudintermedius strains to pradofloxacin. | we investigated in vitro activity of a novel veterinary fluoroquinolone, pradofloxacin, against methicillin-resistant staphylococcus pseudintermedius (mrsp) isolates and compared with other fluoroquinolones. a total of 38 mrsp isolates were subjected to agar disk diffusion tests for sensitivity to pradofloxacin, orbifloxacin, marbofloxacin, enrofloxacin, and ciprofloxacin. the minimal inhibitory concentration (mic) values of pradofloxacin, ciprofloxacin, and enrofloxacin were determined. mutatio ... | 2016 | 27449131 |
| colonization with methicillin-resistant staphylococcus pseudintermedius in multi-dog households: a longitudinal study using whole genome sequencing. | despite a worldwide increase in the presence of methicillin-resistant staphylococcus pseudintermedius (mrsp) in dogs and its potential to cause serious canine health problem, the understanding of the transmission and long-term carriage of mrsp is limited. the objective of this study was to investigate the transmission of mrsp to contact dogs living in multiple dog households where one or more of the dogs had been diagnosed with a clinically apparent infection with mrsp. mrsp carriage was investi ... | 2016 | 27259821 |
| human infections due to staphylococcus pseudintermedius, an emerging zoonosis of canine origin: report of 24 cases. | staphylococcus pseudintermedius has been recently identified as a novel species within the genus staphylococcus, and is commonly associated with infections in dogs. currently, there are few reports of human infections due to this bacterium. | 2016 | 27241371 |
| antimicrobial susceptibility of staphylococcus pseudintermedius colonizing healthy dogs in saskatoon, canada. | this study reports antimicrobial susceptibility of staphylococcus pseudintermedius carried by healthy dogs in saskatoon, and describes changes in antimicrobial resistance since a 2008 study. one hundred healthy dogs presenting to the wellness service at the western college of veterinary medicine were screened for s. pseudintermedius by culturing rectal and pharyngeal swabs. staphylococcus pseudintermedius was identified biochemically and antimicrobial minimum inhibitory concentrations were deter ... | 2016 | 26740701 |
| characterization of staphylococcus pseudintermedius isolated from diseased dogs in lithuania. | the aim of this study was to characterize staphylococcus pseudintermedius for its antimicrobial resistance and virulence factors with a special focus on methicillin-resistant (mrsp) strains isolated from sick dogs in lithuania. clinically sick adult dogs suffering from infections (n=214) and bitches with reproductive disorders (n=36) from kennels were selected for the study. samples (n=192) from the 250 tested (76.8%) dogs were positive for staphylococcus spp. molecular profiling using the speci ... | 2016 | 27096782 |
| biofilm-associated gene expression in staphylococcus pseudintermedius on a variety of implant materials. | to evaluate the expression of biofilm-associated genes in staphylococcus pseudintermedius on multiple clinically relevant surfaces. | 2016 | 27079435 |
| adherence of methicillin-resistant staphylococcus pseudintermedius to suture materials commonly used in small animal surgery. | to evaluate adherence of methicillin-resistant staphylococcus pseudintermedius (mrsp) to 5 suture materials commonly used in small animal surgery. | 2016 | 27027714 |
| efficacy of medical grade honey in the management of canine otitis externa - a pilot study. | the high prevalence of antimicrobial resistance within otic pathogens has created a need for alternative therapies of otitis externa (oe). evidence suggests that medical grade honey (mgh) may be effective against drug-resistant pathogens. | 2016 | 26929137 |
| crystal structure of fhud at 1.6 å resolution: a ferrichrome-binding protein from the animal and human pathogen staphylococcus pseudintermedius. | staphylococcus pseudintermedius is a leading cause of disease in dogs, and zoonosis causes human infections. methicillin-resistant s. pseudintermedius strains are emerging, resembling the global health threat of s. aureus. therefore, it is increasingly important to characterize potential targets for intervention against s. pseudintermedius. here, fhud, an s. pseudintermedius surface lipoprotein implicated in iron uptake, was characterized. it was found that fhud bound ferrichrome in an iron-depe ... | 2016 | 26919525 |
| prior antibacterial drug exposure in dogs with meticillin-resistant staphylococcus pseudintermedius (mrsp) pyoderma. | the emergence of meticillin-resistant staphylococcus pseudintermedius (mrsp) has become a significant animal health problem. recent studies have indicated that previous antibacterial drug exposure is a factor in acquisition of meticillin-resistant strains of staphylococci. | 2016 | 26909526 |
| canine staphylococcus pseudintermedius sinonasal infection in human hosts. | staphylococcus pseudintermedius is a gram-positive bacterium commonly found as part of the normal skin and nasal flora of healthy dogs. it may act as an opportunistic pathogen in dogs, but has also been shown to colonize the nasal mucosa of humans. we report 4 cases of chronic rhinosinusitis (crs) refractory to aggressive medical management with cultures that grew s. pseudintermedius, with clinical improvement only after initiating culture-directed therapy. | 2016 | 26880481 |
| first report of a meca-positive multidrug-resistant staphylococcus pseudintermedius isolated from a dog in new zealand. | a 14-year-old neutered male sealyham terrier was referred for assessment of a persistent pyoderma. it had experienced numerous episodes of dermatitis involving pododermatitis, pyoderma and otitis over the previous 6 years. | 2016 | 26850500 |
| identification of vaccine candidate antigens of staphylococcus pseudintermedius by whole proteome characterization and serological proteomic analyses. | the recent emergence of methicillin-resistant staphylococcus pseudintermedius (mrsp) has complicated considerably the treatment of infections caused by these bacteria. therefore new treatment strategies are urgently needed, namely through the development of vaccines towards the control of bacterial infections. our study describes an extensive characterization of the proteome of s. pseudintermedius through a 2-de maldi-tof/tof approach, followed by serological proteome analysis (serpa) to identif ... | 2016 | 26721443 |
| characterization of a novel composite staphylococcal cassette chromosome mec in methicillin-resistant staphylococcus pseudintermedius from thailand. | a novel staphylococcal cassette chromosome mec (sccmec) composite island (sccmecai16-sccczrai16-ci) was identified in staphylococcus pseudintermedius. four integration site sequences for scc subdivided the 60,734-bp island into 41,232-bp sccmecai16, 19,400-bp sccczrai16, and 102-bp scc-likeai16 elements. sccmecai16 represents a new combination of ccra1b3 genes with a class a mec complex. sccczrai16 contains ccra1b6 and genes related to restriction modification and heavy metal resistance. sccmeca ... | 2016 | 26643350 |
| comparative rna-seq-based transcriptome analysis of the virulence characteristics of methicillin-resistant and -susceptible staphylococcus pseudintermedius strains isolated from small animals. | staphylococcus pseudintermedius is often associated with pyoderma, which can turn into a life-threatening disease. the dissemination of highly resistant isolates has occurred in the last 10 years and has challenged antimicrobial treatment of these infections considerably. we have compared the carriage of virulence genes and biofilm formation between methicillin-resistant and methicillin-susceptible s. pseudintermedius (mrsp and mssp, respectively) isolates and their in vitro gene expression prof ... | 2016 | 26621622 |
| occurrence and characterization of inducible clindamycin resistance in canine methicillin-resistant staphylococcus pseudintermedius. | this study aimed to detect inducible clindamycin (icli) resistance in staphylococcus pseudintermedius isolated from dogs in thailand using d-zone testing. strains that were icli-resistant were characterized by molecular typing and antibiogram and were detected in 10/200 s. pseudintermedius isolates (5%) from 7/41 dogs (17%). all were methicillin-resistant s. pseudintermedius (mrsp) and demonstrated multidrug resistance. the icli-resistant mrsp contained erm(b) and had identical or closely relate ... | 2016 | 26616424 |
| changes in the population of methicillin-resistant staphylococcus pseudintermedius and dissemination of antimicrobial-resistant phenotypes in the netherlands. | methicillin-resistant staphylococcus pseudintermedius (mrsp), which is often multidrug resistant (mdr), has recently emerged as a threat to canine health worldwide. knowledge of the temporal distribution of specific mrsp lineages, their antimicrobial resistance phenotypes, and their association with clinical conditions may help us to understand the emergence and spread of mrsp in dogs. the aim of this study was to determine the yearly proportions of mrsp lineages and their antimicrobial-resistan ... | 2016 | 26582835 |
| detection of methicillin-resistant staphylococcus pseudintermedius st169 and novel st354 sccmec ii-iii isolates related to the worldwide st71 clone. | the recent appearance of methicillin-resistant staphylococcus pseudintermedius (mrsp) is a concern for both veterinary and human healthcare. mrsp clonal lineages with sequence type (st) 71-spa t02-staphylococcal cassette chromosome mec (sccmec) ii-iii and st68-spa t06-sccmec v have spread throughout europe and north america, respectively. the current study compared the molecular characteristics of 43 mrsp isolates from dogs in japan with those of mrsp from previous reports using multilocus seque ... | 2016 | 26138564 |
| 40 years of veterinary papers in jac - what have we learnt? | this review, for the occasion of the 40th anniversary of the journal of antimicrobial chemotherapy (jac), gives an overview of the manuscripts related to veterinary bacteriology published in the journal in the past 40 years with a focus on 'one health' aspects. from 1975 to 2000 the number of manuscripts related to veterinary medicine was limited, but thereafter, the number steadily increased. most manuscripts published were related to food-producing animals, but companion animals and minor spec ... | 2016 | 27660260 |
| prior antimicrobial use as a risk factor for resistance in selected staphylococcus pseudintermedius isolates from the skin and ears of dogs. | antimicrobial resistance within bacteria continues to present therapeutic challenges. one presumed risk factor for increased rates of resistance is prior exposure to antimicrobial drugs. | 2016 | 27870236 |
| carriage rate and antibiotic susceptibility of coagulase-positive staphylococci isolated from healthy dogs in victoria, australia. | studies in australia and elsewhere have shown high levels of antibiotic resistance in coagulase-positive staphylococci in dogs visiting veterinary clinics with pyoderma and related conditions. although important, such studies tend to overestimate the burden of resistance. the aim of the current study was to investigate the prevalence of coagulase-positive staphylococci in healthy dogs in central victoria to assess the level of antibiotic resistance among these isolates. | 2016 | 27804112 |
| complete genome sequences of three important methicillin-resistant clinical isolates of staphylococcus pseudintermedius. | we report the first complete genome sequences of three predominant clones (st68, st71, and st84) of methicillin-resistant staphylococcus pseudintermedius in north america. all strains were isolated from canine infections and have different sccmec elements and antibiotic resistance gene patterns. | 2016 | 27795289 |
| exploring staphylococcus pseudintermedius: an emerging zoonotic pathogen? | 2016 | 27750440 | |
| in vitro antimicrobial susceptibility of staphylococcus pseudintermedius isolates of human and animal origin. | mic results for 115 staphylococcus intermedius group isolates are presented. of these, 33% were methicillin resistant, among which 51.4% were susceptible to doxycycline, 29.7% to clindamycin, and 21.6% to trimethoprim-sulfamethoxazole. all of the isolates were susceptible to ceftaroline, daptomycin, linezolid, nitrofurantoin, quinupristin-dalfopristin, rifampin, tigecycline, and vancomycin. of all the isolates, 82.6%, 67.8%, and 23.5% were susceptible to ciprofloxacin, erythromycin, and penicill ... | 2016 | 26962087 |
| what's in a name? the impact of accurate staphylococcus pseudintermedius identification on appropriate antimicrobial susceptibility testing. | bacteria in the staphylococcus intermedius group, including staphylococcus pseudintermedius, often encode meca-mediated methicillin resistance. reliable detection of this phenotype for proper treatment and infection control decisions requires that these coagulase-positive staphylococci are accurately identified and specifically that they are not misidentified as s. aureus. as correct species level bacterial identification becomes more commonplace in clinical laboratories, one can expect to see c ... | 2016 | 26763965 |
| linoleic acid salt with ultrapure soft water as an antibacterial combination against dermato-pathogenic staphylococcus spp. | skin colonization of staphylococcus spp. critically affects the severity of dermatitis in humans and animals. we examined different types of fatty acid salts for their antibacterial activity against staphylococcus spp. when used in ultrapure soft water (upsw). we also evaluated their therapeutic effect on a spontaneous canine model of dermatitis. | 2016 | 26606689 |
| the immunopathogenesis of staphylococcal skin infections - a review. | staphylococcus aureus and s. pseudintermedius are the major causes of bacterial skin disease in humans and dogs. these organisms can exist as commensals on the skin, but they can also cause severe or even devastating infections. the immune system has evolved mechanisms to deal with pathogenic microorganisms and has strategies to combat bacteria of this type. what emerges is a delicate "peace" between the opposing sides, but this balance can be disrupted leading to a full blown "war". in the fero ... | 2016 | 27865269 |
| comparison of the in vitro activity of five antimicrobial drugs against staphylococcus pseudintermedius and staphylococcus aureus biofilms. | resistance in canine pathogenic staphylococci is necessitating re-evaluation of the current antimicrobial treatments especially for biofilm-associated infections. long, repeated treatments are often required to control such infections due to the tolerance of bacteria within the biofilm. to comply with the goal of better antibiotic stewardship in veterinary medicine, the efficacies of the available drugs need to be directly assessed on bacterial biofilms. we compared the activities of amoxicillin ... | 2016 | 27531995 |
| searching for beta-haemolysin hlb gene in staphylococcus pseudintermedius with species-specific primers. | the paper presents an analysis of 51 staphylococcus pseudintermedius clinically isolated strains from humans and from animals. staphylococcus pseudintermedius strains' ability to produce β-haemolysin was evaluated with phenotypic methods (hot-cold effect, reverse camp test). in order to determine the hlb gene presence (coding for β-haemolysin) in a genomic dna, pcr reactions were conducted with two different pairs of primers: one described in the literature for staphylococcus aureus and recommen ... | 2016 | 27086303 |
| trends and molecular mechanisms of antimicrobial resistance in clinical staphylococci isolated from companion animals over a 16 year period. | the objective of this study was to investigate the evolution of resistance to antimicrobials, corresponding mechanisms and molecular characteristics of staphylococcus spp., between 1999 and 2014. | 2016 | 26944924 |
| evaluation of oxacillin and cefoxitin disk and mic breakpoints for prediction of methicillin resistance in human and veterinary isolates of staphylococcus intermedius group. | staphylococcus pseudintermedius is a coagulase-positive species that colonizes the nares and anal mucosa of healthy dogs and cats. human infections with s. pseudintermedius range in severity from bite wounds and rhinosinusitis to endocarditis; historically, these infections were thought to be uncommon, but new laboratory methods suggest that their true incidence is underreported. oxacillin and cefoxitin disk and mic tests were evaluated for the detection of meca- or mecc-mediated methicillin res ... | 2016 | 26607988 |
| assessment of wound bio-burden and prevalence of multi-drug resistant bacteria during open wound management. | to describe the bacterial bio-burden of open-treated wounds and make comparisons with bite wounds. | 2016 | 27101415 |
| clinical features, cytology and bacterial culture results in dogs with and without cheilitis and comparison of three sampling techniques. | cheilitis is a common presentation in dogs associated with a variety of skin diseases and often complicated by microbial infections. | 2016 | 27019393 |
| antibacterial effect of n-acetylcysteine on common canine otitis externa isolates. | n-acetylcysteine (nac) has the potential to be a useful therapeutic agent for the treatment of otitis externa due to its antimicrobial and mucolytic properties, as well as its ability to disrupt bacterial biofilm. | 2016 | 27122224 |
| pathophysiological mechanisms of staphylococcus non-aureus bone and joint infection: interspecies homogeneity and specific behavior of s. pseudintermedius. | implicated in more than 60% of bone and joint infections (bjis), staphylococci have a particular tropism for osteoarticular tissue and lead to difficult-to-treat clinical infections. to date, staphylococcus aureus internalization in non-professional phagocytic cells (nppcs) is a well-explored virulence mechanism involved in bji chronicity. conversely, the pathophysiological pathways associated with staphylococcus non-aureus (sna) bjis have scarcely been studied despite their high prevalence. in ... | 2016 | 27462303 |
| antimicrobial susceptibility monitoring of dermatological bacterial pathogens isolated from diseased dogs and cats across europe (compath results). | the compath project is a pan-european programme dedicated to the monitoring of antimicrobial susceptibility of pathogens from diseased dogs and cats using standardized methods and centralized minimum inhibitory concentration (mic) determination. here, the susceptibility of major pathogens is reported from antimicrobial nontreated animals with acute clinical signs of skin, wound or ear infections in 2008-2010. | 2016 | 27593360 |
| prevalence and resistance patterns of canine uropathogens in regard to concurrent diseases. | predisposing factors for different types of urinary tract infections (uti) were evaluated and prevalence of causative agents and their resistance were identified. a prospective epidemiologic study (2007 to 2012) included 191 dogs with signs of urinary tract disease. anamnestic data were collected and clinical examination, abdominal ultrasonography, urinalysis and aerobic bacteriologic urine culture were performed in all dogs. other diagnostic procedures were conducted when indicated. uti was mor ... | 2016 | 27529997 |
| comparative in vitro killing of canine strains of staphylococcus pseudintermedius and escherichia coli by cefovecin, cefazolin, doxycycline and pradofloxacin. | bacterial eradication is necessary for clinical cure of infections and antimicrobial agents are important adjunctive therapies for inhibiting the growth of or killing bacteria. pre-existing skin diseases predispose animals to infection by staphylococcus pseudintermedius and, more rarely, by gram-negative bacilli. the property of rapid killing of bacteria may influence drug selection and duration of therapy in the setting of infection. | 2016 | 27307405 |
| systematic review on global epidemiology of methicillin-resistant staphylococcus pseudintermedius: inference of population structure from multilocus sequence typing data. | background and rationale: methicillin-resistant staphylococcus pseudintermedius (mrsp) is a major cause of infections in dogs, also posing a zoonotic risk to humans. this systematic review aimed to determine the global epidemiology of mrsp and provide new insights into the population structure of this important veterinary pathogen. methodology: web of science was searched systematically for articles reporting data on multilocus sequence typing (mlst) of s. pseudintermedius isolates from dogs or ... | 2016 | 27803691 |
| canine influenza virus coinfection with staphylococcus pseudintermedius enhances bacterial colonization, virus load and clinical presentation in mice. | canine influenza virus (civ) and staphylococcus pseudintermedius (sp) are pathogens that cause respiratory disease in dogs. considering bacterial infections following influenza are a leading cause of illness and death, it is of particular meaning to investigate the interaction between these two pathogens. in this study, balb/c mice were used as a mouse model to assess whether inoculation with civ h3n2 followed by s. pseudintermedius 72 h later resulted in exacerbation of disease. disease was cha ... | 2016 | 27259293 |
| sequence-based characterization of tn5801-like genomic islands in tetracycline-resistant staphylococcus pseudintermedius and other gram-positive bacteria from humans and animals. | antibiotic resistance in pathogens is often associated with mobile genetic elements, such as genomic islands (gi) including integrative and conjugative elements (ices). these can transfer resistance genes within and between bacteria from humans and/or animals. the aim of this study was to investigate whether tn5801-like gis carrying the tetracycline resistance gene, tet(m), are common in staphylococcus pseudintermedius from pets, and to do an overall sequences-based characterization of tn5801-li ... | 2016 | 27199912 |
| can ultraviolet light c decrease the environmental burden of antimicrobial-resistant and -sensitive bacteria on textiles? | the environment is important in transmission of bacteria. textiles are difficult and time consuming to clean; ultraviolet light c (uvc) is germicidal and may be an effective disinfection method for textile surfaces. | 2016 | 27620856 |
| a pilot study comparing in vitro efficacy of topical preparations against veterinary pathogens. | topical antimicrobial agents are important for the management of cutaneous infections. for topical antimicrobial agents, in vitro efficacy data are limited. | 2016 | 27109449 |
| is transmission electron microscopy (tem) a promising approach for qualitative and quantitative investigations of polymyxin b and miconazole interactions with cellular and subcellular structures of staphylococcus pseudintermedius, escherichia coli, pseudomonas aeruginosa and malassezia pachydermatis? | antimicrobial therapy using a combination of polymyxin b and miconazole is effective against the main bacterial pathogens associated with otitis externa in dogs, and a synergistic effect of both drugs has been shown previously. the objective of the present investigation was to visualize ultrastructural changes after exposure of escherichia coli, pseudomonas aeruginosa, staphylococcus pseudintermedius and malassezia pachydermatis to polymyxin b and miconazole by transmission electron microscopic ... | 2015 | 26527257 |
| optimization and evaluation of flexicult® vet for detection, identification and antimicrobial susceptibility testing of bacterial uropathogens in small animal veterinary practice. | urinary tract infection (uti) is a common reason for antimicrobial prescription in dogs and cats. the objective of this study was to optimize and evaluate a culture-based point-of-care test for detection, identification and antimicrobial susceptibility testing of bacterial uro-pathogens in veterinary practice. | 2015 | 26503106 |
| oxacillin sensitization of methicillin-resistant staphylococcus aureus and methicillin-resistant staphylococcus pseudintermedius by antisense peptide nucleic acids in vitro. | antibiotic resistance genes can be targeted by antisense agents, which can reduce their expression and thus restore cellular susceptibility to existing antibiotics. antisense inhibitors can be gene and pathogen specific, or designed to inhibit a group of bacteria having conserved sequences within resistance genes. here, we aimed to develop antisense peptide nucleic acids (pnas) that could be used to effectively restore susceptibility to β-lactams in methicillin-resistant staphylococcus aureus (m ... | 2015 | 26560174 |
| a bioengineered nisin derivative to control biofilms of staphylococcus pseudintermedius. | antibiotic resistance and the shortage of novel antimicrobials are among the biggest challenges facing society. one of the major factors contributing to resistance is the use of frontline clinical antibiotics in veterinary practice. in order to properly manage dwindling antibiotic resources, we must identify antimicrobials that are specifically targeted to veterinary applications. nisin is a member of the lantibiotic family of antimicrobial peptides that exhibit potent antibacterial activity aga ... | 2015 | 25789988 |
| fibronectin binding proteins spsd and spsl both support invasion of canine epithelial cells by staphylococcus pseudintermedius. | in this study, we investigated the cell wall-anchored fibronectin-binding proteins spsd and spsl from the canine commensal and pathogen staphylococcus pseudintermedius for their role in promoting bacterial invasion of canine progenitor epidermal keratinocytes (cpek). invasion was examined by the gentamicin protection assay and fluorescence microscopy. an δspsd δspsl mutant of strain ed99 had a dramatically reduced capacity to invade cpek monolayers, while no difference in the invasion level was ... | 2015 | 26238710 |
| the distribution of pathogens and their antimicrobial susceptibility patterns among canine surgical wound infections in sweden in relation to different risk factors. | surgical site infection (ssi) is a common nosocomial infection in dogs and a growing concern in veterinary hospitals as an increase in multidrug-resistant pathogens is reported. despite the need for rational and prudent antimicrobial use, few peer-reviewed and published veterinary studies have investigated the pathogenic growth including susceptibility patterns of the isolated pathogens in canine ssis. the first objective of the present study was to estimate the distribution of bacterial pathoge ... | 2015 | 25886937 |
| in vitro assessment of chloramphenicol and florfenicol as second-line antimicrobial agents in dogs. | the aim of this study was to evaluate the potential of chloramphenicol and florfenicol as second-line antimicrobial agents for treatment of infections caused by methicillin-resistant staphylococcus pseudintermedius (mrsp) and extended-spectrum β-lactamase (esbl)-producing escherichia coli in dogs, through a systematic in vitro assessment of the pharmacodynamic properties of the two drugs. minimum inhibitory concentrations (mic) and phenicol resistance genes were determined for 169 s. pseudinterm ... | 2015 | 25623169 |
| prevalence and risk factors for isolation of methicillin-resistant staphylococcus in dogs with keratitis. | to determine the prevalence of, and risk factors for, methicillin-resistant staphylococcus (mrs) isolation in dogs with naturally acquired bacterial keratitis. | 2015 | 25130050 |
| staphylococcus pseudintermedius can be misdiagnosed as staphylococcus aureus in humans with dog bite wounds. | the purpose of this study was to investigate whether s. pseudintermedius is misdiagnosed as s. aureus by clinical laboratories when isolated from humans with dog bite wounds. in addition, we attempted to determine whether s. pseudintermedius isolates related to dog bite wounds share phenotypic and genotypic traits. s. pseudintermedius was identified by pcr targeting the nuc gene. isolates were tested for antibiotic susceptibility using vetmic gp-mo microdilution panels. the occurrence of genes e ... | 2015 | 25532507 |
| first identification of methicillin-resistant staphylococcus pseudintermedius strains among coagulase-positive staphylococci isolated from dogs with otitis externa in trinidad, west indies. | otitis externa is a common inflammatory ear disease in dogs caused by a variety of pathogens, and coagulase-positive staphylococci are frequently isolated from such infections. | 2015 | 26715379 |
| detection and sequence analysis of accessory gene regulator genes of staphylococcus pseudintermedius isolates. | staphylococcus pseudintermedius (sp) is the major pathogenic species of dogs involved in a wide variety of skin and soft tissue infections. the accessory gene regulator (agr) locus of staphylococcus aureus has been extensively studied, and it influences the expression of many virulence genes. it encodes a two-component signal transduction system that leads to down-regulation of surface proteins and up-regulation of secreted proteins during in vitro growth of s. aureus. the objective of this stud ... | 2015 | 27047173 |
| epidemiology of methicillin resistant staphylococcus pseudintermedius in guide dogs in finland. | methicillin resistant staphylococcus pseudintermedius (mrsp) and staphylococcus aureus (mrsa) are common multi-drug resistant (mdr) bacteria in dogs. in 2012-2013 three dogs of the guide dog school of the finnish federation of the visually impaired were found to be mrsp positive. guide dogs have regular contact with each other during their first year of life and prolonged contact when in training. since dogs are placed in different parts of finland after training, there is a risk for national sp ... | 2015 | 26183814 |
| antibacterial characterization of novel synthetic thiazole compounds against methicillin-resistant staphylococcus pseudintermedius. | staphylococcus pseudintermedius is a commensal organism of companion animals that is a significant source of opportunistic infections in dogs. with the emergence of clinical isolates of s. pseudintermedius (chiefly methicillin-resistant s. pseudintermedius (mrsp)) exhibiting increased resistance to nearly all antibiotic classes, new antimicrobials and therapeutic strategies are urgently needed. thiazole compounds have been previously shown to possess potent antibacterial activity against multidr ... | 2015 | 26086336 |