Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| efficacy of oil-emulsion vaccines prepared with pigeon paramyxovirus-1, ulster, and la sota newcastle disease viruses. | three strains of avian paramyxovirus-1 virus (pmv-1) were used to prepare four experimental monovalent oil-emulsion vaccines. a pigeon pmv-1 isolate (ppmv-1) and the newcastle disease virus strains la sota and ulster were used to prepare four pools of beta-propiolactone-inactivated allantoic fluid for the vaccines. groups of susceptible white rock chickens and racing homing pigeons were vaccinated subcutaneously with one of the vaccines, and their serologic responses were determined using the he ... | 1989 | 2522767 |
| priming for local and systemic antibody memory responses to bovine respiratory syncytial virus: effect of amount of virus, virus replication, route of administration and maternal antibodies. | we studied the conditions under which calves can be primed for mucosal and serum antibody memory responses against bovine respiratory syncytial virus (brsv), and the relationship between such responses and protection against the virus. calves were primed via the respiratory tract with a low or high amount of live virus, with killed virus, or intramuscularly with live virus. calves were challenged via the respiratory tract. priming with live virus via the respiratory tract induced primary antibod ... | 1989 | 2530685 |
| t-cells in respiratory syncytial virus (rsv) infection. | 1989 | 2533461 | |
| enzyme immunoassays for detection of igg and igm antibodies to parainfluenza types 1, 2 and 3. | quantitative enzyme immunoassays for parainfluenza type 1, 2 and 3 igg antibodies were developed. serum specimens were tested at a single dilution of 1:1000 and results expressed in units by the use of a standard curve. the unit values correlated well with titres obtained by testing the same specimens in serial dilutions. all serum pairs with significant titre rises also showed significant rises in unit values. parainfluenza igg and igm serology was evaluated in 66 patients with a proven parainf ... | 1989 | 2536382 |
| analysis of the in vitro antiviral activity of certain ribonucleosides against parainfluenza virus using a novel computer aided receptor modeling procedure. | the in vitro antiviral activity of 28 nucleosides against the parainfluenza virus type 3 has been analyzed by using a novel computer aided receptor modeling procedure. the method involves an extensive modification of our earlier work (ghose, a. k.; crippen, g. m. j. med. chem. 1985, 28, 333). it presents a more straightforward algorithm for the steps that suffered from subjectivity in the earlier method. the method first determines the possible low-energy conformations of the nucleosides, and as ... | 1989 | 2539476 |
| expression of the fusion glycoprotein of human parainfluenza type 3 virus in insect cells by a recombinant baculovirus and analysis of its immunogenic property. | the fusion (f) glycoprotein of human parainfluenza type 3 (pi3) virus was produced in insect cells using a baculovirus expression vector (pacym1). the recombinant glycoprotein was identified by its reactivity with specific monoclonal and polyclonal antibodies and showed an apparent molecular mass of 70 kda. although the fusion protein was found on the infected cell surface, it did not appear to be proteolytically cleaved to f1 and f2 subunits. immunization of hamsters with the recombinant protei ... | 1989 | 2539707 |
| effects of vaccines on the canine immune system. | the effects of several commercially available polyvalent canine vaccines on the immune system of the dog were examined. the results demonstrated that the polyvalent vaccines used in this study significantly suppressed the absolute lymphocyte count and that most of the polyvalent vaccines significantly suppressed lymphocyte response to mitogen, but had no effect on natural effector cell activity, neutrophil chemiluminescence, nor antibody response to canine distemper virus. the individual vaccine ... | 1989 | 2540897 |
| evaluation of a monoclonal antibody pool for rapid diagnosis of respiratory viral infections. | a pool of monoclonal antibodies (mabp) was evaluated both as a method of cell culture confirmation and as a rapid diagnostic screen for viral infection in respiratory secretions. the mabp was used in a two-step fluorescent staining procedure on cells harvested from cultures (phase 1) and on exfoliated nasopharyngeal or tracheal cells (phase 2). antibodies in the mabp were directed against respiratory syncytial virus, adenoviruses, parainfluenza virus types 1, 2, and 3, and influenza viruses a an ... | 1989 | 2541165 |
| paramyxovirus sv5 and multiple sclerosis. | multiple sclerosis is commonly associated with a local humoral immune response within the central nervous system. a hallmark of this intrathecal response is the presence of electrophoretically demonstrable oligoclonal bands of igg in the cerebrospinal fluid (csf) of up to 95% of patients. observations indicating that a major part of the csf igg in some patients may represent antibodies to sv5, a simian virus closely related to human parainfluenza type 2 virus, were recently reported by goswami e ... | 1989 | 2541340 |
| efficacy of viral components of a nonabortigenic combination vaccine for prevention of respiratory and reproductive system diseases in cattle. | efficacy and safety of components of an im-administered vaccine for prevention of infectious bovine rhinotracheitis virus (ibrv), parainfluenza type-3 (pi-3) virus, bovine viral diarrhea virus (bvdv), and respiratory syncytial virus (rsv) infections and campylobacteriosis and leptospirosis were evaluated in cattle, including calves and pregnant cows. challenge of immunity tests were conducted in calves for ibrv, pi-3 virus, or bvdv vaccinal components. all inoculated calves developed serum-neutr ... | 1989 | 2542202 |
| [experience using dimephosphon in the complex treatment of pneumonia in newborn infants]. | 1989 | 2542878 | |
| [mechanism of penetration of human type 3 parainfluenza virus into monkey kidney cells]. | the mechanism of human parainfluenza type 3 virus penetration into monkey kidney cells was studied by morphological and biochemical methods. the results of electron microscopic studies permit a conclusion that the virus penetrates into the cells by the mechanism of receptor endocytosis. analysis of subvirus structures in cytosole revealed two types of particles: nucleocapsids and structures of a larger size and lower buoyant density containing, in addition to np protein, matrix (m) protein. it i ... | 1989 | 2543139 |
| detection of viral antigens in patients with iga nephropathy. | immunofluorescent analysis of viral antigens in the cultured fibroblasts and renal tissues in patients with iga nephropathy was described. freeze and thawed extracts of pharyngeal cells obtained from patients with iga nephropathy, chronic proliferative glomerulonephritis without iga deposition (pgn) and healthy adults were cultured with human fibroblasts, i.e. hel cells, with or without addition of 5-iodine 2'-deoxy-uridine (iudr) at 37 degrees c for 2 weeks. these fibroblasts and renal sections ... | 1989 | 2543852 |
| epidemiology of parainfluenza virus type 3 in england and wales over a ten-year period. | we have analysed data on respiratory syncytial (rs) and parainfluenza type 3 (pf3) viruses reported to the communicable disease surveillance centre, london, over the period 1978-87. these confirm the annual winter epidemic of rs virus and show that, in england and wales, pf3 is a summer infection with regular yearly epidemics. | 1989 | 2544442 |
| virus-specific polypeptides of human parainfluenza virus type 4 and their synthesis in infected cells. | we have studied the structural components of human parainfluenza virus type 4a (piv-4a) and identified some virus-specific polypeptides by immunoprecipitation with polyclonal and monoclonal antibodies followed by one- or two-dimensional sds-page. hn polypeptides existed as monomer, disulfide-linked dimer, and disulfide-linked larger oligomer in cells infected with piv-4a. interestingly, the nonreduced np, the nonreduced fusion, and the reduced f1 proteins migrated as doublets. two f1 polypeptide ... | 1989 | 2545032 |
| extensive antigenic diversity among human parainfluenza type 2 virus isolates and immunological relationships among paramyxoviruses revealed by monoclonal antibodies. | a panel of 128 monoclonal antibodies (mabs) directed against hemagglutinin-neuraminidase (hn), fusion (f), matrix (m), and polymerase (p) proteins, and nucleoprotein (np) of the toshiba strain of human parainfluenza type 2 virus (piv2) was prepared to examine the antigenic relationships among clinical isolates of piv2 and among paramyxoviruses by indirect enzyme-linked immunosorbent assays. the hn proteins of 18 clinical isolates of piv2 showed extensive antigenic diversity: 23 of 33 anti-hn mab ... | 1989 | 2545038 |
| clinical manifestations of respiratory tract infections due to respiratory syncytial virus and rhinoviruses in hospitalized children. | from september 1984 to may 1986, nasopharyngeal secretions were obtained from 519 children with some form of respiratory tract infection. the nasal secretions were screened for respiratory syncytial virus (rsv), rhinoviruses, adenoviruses, parainfluenza virus types 1, 2, 3, influenza virus types a and b, and enteroviruses by tissue culture virus isolation technique and/or enzyme-linked immunosorbent assay. a uniform questionnaire gave information about age, sex, individual signs and symptoms, fi ... | 1989 | 2545074 |
| comparison of monoclonal antibody time-resolved fluoroimmunoassay with monoclonal antibody capture-biotinylated detector enzyme immunoassay for respiratory syncytial virus and parainfluenza virus antigen detection. | an all-monoclonal antibody, time-resolved fluoroimmunoassay was compared with several enzyme immunoassays for the detection of respiratory syncytial virus and parainfluenza virus type 1, 2, and 3 antigens in clinical specimens. the most sensitive enzyme immunoassay for parainfluenza virus type 1 was an all-monoclonal antibody assay with biotin-labeled detector antibody and streptavidin-peroxidase conjugate, but for respiratory syncytial virus and parainfluenza virus types 2 and 3 the most sensit ... | 1989 | 2546973 |
| comparison of three techniques for detection of respiratory viruses in nasopharyngeal aspirates from children with lower acute respiratory infections. | a comparison of immunofluorescence (if), enzyme-linked immunosorbent assay (elisa), and isolation in tissue culture (tc) for detection of respiratory viruses was performed on 496 nasopharyngeal aspirates from children under 5 years of age with lower acute respiratory infections who were receiving attention at three hospitals in buenos aires, argentina. all samples were tested by the three methods for respiratory syncytial virus (rsv), influenza a and b, adenovirus, and parainfluenza 1 and 3. vir ... | 1989 | 2547019 |
| syncytium formation by recombinant vaccinia viruses carrying bovine parainfluenza 3 virus envelope protein genes. | the highly syncytium-inducing m strain and the weakly syncytium-inducing sc strain of bovine parainfluenza 3 virus differ by a single amino acid substitution in each of the hemagglutinin-neuraminidase (hn) and membrane (m) proteins, while their fusion (f) proteins are identical (t. shioda, s. wakao, s. suzu, and h. shibuta, virology 162:388-396, 1988). we constructed recombinant vaccinia viruses which express separately the m virus hn (vac-mhn), sc virus hn (vac-schn), m virus m (vac-mm), sc vir ... | 1989 | 2547990 |
| evidence for australia-wide canine parainfluenza infection. | 1989 | 2549930 | |
| [nosospecific features of the dynamics of immunological parameters in young children with acute respiratory virus infections of varying etiology]. | the study of acute viral respiratory infections has shown that in the course of these infections fluctuations in the content of antigen-nonspecific immunocytes and immunoglobulins occur, depending on the etiology of the disease. this dependence should be taken into consideration in the evaluation of the immune status of young children with acute viral respiratory infections. | 1989 | 2552713 |
| broad-spectrum antiviral activities of neplanocin a, 3-deazaneplanocin a, and their 5'-nor derivatives. | the neplanocin a analogs, 3-deazaneplanocin a, 9-(trans-2',trans-3'-dihydroxycyclopent-4'-enyl)adenine (dhca), and 9-(trans-2',trans-3'-dihydroxycyclopent-4'-enyl)-3-deazaadenine (dhcda), all potent inhibitors of s-adenosylhomocysteine (adohcy) hydrolase, were studied for their broad-spectrum antiviral potential. 3-deazaneplanocin a, dhca, and dhcda proved specifically effective against vesicular stomatitis virus, vaccinia virus, parainfluenza virus, reovirus, and rotavirus. their selectivity wa ... | 1989 | 2552906 |
| influence of viral infections on body weight, survival, and tumor prevalence in fischer 344/ncr rats on two-year studies. | sendai virus (sv), pneumonia virus of mice (pvm), and rat coronavirus/sialodacryoadenitis virus (rcv/sdav) were common viral infections of rats in the national cancer institute-national toxicology program (nci-ntp) studies from 1977 to 1983. influence of these viral infections on body weight, survival, and prevalences of spontaneous tumors in the f344/ncr rats of 28 diet control groups at five different laboratories were evaluated. tumor prevalences evaluated in this investigation included the f ... | 1989 | 2554057 |
| the role of viral infections in intrinsic asthma: activation of neutrophil inflammation. | 1989 | 2554699 | |
| oral medicine in practice: viral infection. | the fourth article in this series deals with viral infections which may present in the dental surgery. two groups of viruses, herpes and coxsackie, are responsible for the majority of viral conditions involving the orofacial region which present to the dentist. mucosal ulceration is the most frequent clinical presentation, although viruses may also be responsible for salivary gland swelling or epithelial hyperplasia. diagnosis of viral infection is important, since treatment which can alleviate ... | 1989 | 2556171 |
| parainfluenza 3 vaccination of sheep. | outbreaks of pneumonia associated with pasteurella haemolytica have occurred in sheep in the area of perthshire served by this practice, and on some farms the disease has been an important annual cause of loss. serological evidence was obtained that parainfluenza 3 (pi3) virus might be implicated as a predisposing factor to pasteurellosis. a live attenuated pi3 virus vaccine licensed for use in cattle was given intranasally to ewes on one farm. many sheep seroconverted and outbreaks of pneumonia ... | 1989 | 2556831 |
| [quantitative relationship between fatty acids comprising lipids of viruses and cells]. | 1989 | 2558450 | |
| epidemiologic survey of bovine diseases in suriname. | a seroepidemiologic survey of cattle diseases was undertaken in suriname in 1985 to help assess the livestock disease situation in that country. the six diseases covered by the survey were bovine coronavirus infection, bovine rhinotracheitis, bovine virus diarrhea, brucellosis, parainfluenza-3 infection, and respiratory syncytial virus infection. the results indicated relatively low prevalences of these diseases compared to the prevalences found in most developed countries. the reasons for this ... | 1989 | 2558753 |
| parainfluenza virus infection of cultured airway epithelial cells. | studies of the cellular effects of respiratory viruses have generally used cultures of non-airway (particularly renal) epithelial cells. this requires the assumption that, despite the marked differences between renal epithelium and airway epithelium, the virus-host cell interactions in cultures of renal epithelium will be relevant to those in airway epithelium. to study viral infection of airway epithelial cells, we removed the epithelial cells from ferret tracheas using 0.1% pronase solution, a ... | 1989 | 2559102 |
| lack of an association between acute gastroenteritis, acute respiratory infections and malaria in young gambian children. | the incidence of acute gastrointestinal and acute respiratory infections was measured in 2 groups of approximately 750 gambian children aged 3-59 months during a 3-year period. one group of children was partially protected against malaria by fortnightly chemoprophylaxis with maloprim whilst children in the other group were infected much more frequently. mortality from acute gastroenteritis and from acute respiratory infections was similar in the 2 groups. the proportions of children in each grou ... | 1989 | 2559509 |
| [serological investigation on infant virus pneumonia in beijing]. | antibody against adenovirus in paired sera of 171 cases of infant virus pneumonia observed in 7 winter-spring periods was assayed by hemagglutination-inhibition test and enzyme-linked immunosorbent assay. a 4-fold or greater rise in the titer was found in 56 cases (32.75%). type 3 and type 7 were predominant. antibody against influenza virus was assayed in paired sera of 129 cases in 6 winter-spring periods. a 4-fold or greater increase in the titer was observed in 11 cases (8.53%), with predomi ... | 1989 | 2560411 |
| an evaluation of four serological tests for the detection of antibodies to bovine parainfluenza virus type 3. | haemagglutination-inhibition (hi), virus neutralization (vn), haemolysis-inhibition (hli-1, hli-2) tests, and two new haemofusion-inhibition (hfi-1, hfi-2) tests, developed by us, were tested for the detection of antibodies to bovine parainfluenza virus type 3 (bpiv-3) in sera of 45 steers randomly selected from a herd naturally infected by bpiv-3. twelve seronegative animals were then vaccinated with formalin-inactivated vaccine and 15 days later tested by the above-mentioned assays. linear reg ... | 1989 | 2561663 |
| comparative virulence of respiratory syncytial virus subgroups a and b. | 1989 | 2564579 | |
| effects of norakin on respiratory syncytial virus in tissue culture and in mice. | norakin at 1 microgram/ml inhibits the reproduction of respiratory syncytial virus (rsv) in vero cells to 50% and at 5 micrograms/ml to 90%. the development of lung lesions in rsv-infected balb/c mice was suppressed by 70% when the animals were treated with two doses (25 mg/kg each) of norakin, 30 min before and 4 hr post infection. (p.i.), respectively. | 1989 | 2569812 |
| respiratory syncytial virus subgroups and pneumonia in children. | 1989 | 2572768 | |
| antigenic analysis of a putative new strain of respiratory syncytial virus. | a recent isolate (9320) of respiratory syncytial virus (rsv) that had been reported to differ from all previous strains was compared with rsv strains isolated over a period of 23 years by using the techniques of in vitro neutralization by specific antiserum and in vivo resistance to infection in cotton rats. the 9320 strain did not appear to be a novel antigenic variant and exhibited greater homology to several prototype strains than did an earlier isolate. the antigenic differences detected by ... | 1985 | 2579170 |
| outbreak of the pigeon paramyxovirus serotype 1 in the sudan. | 1985 | 2581357 | |
| respiratory syncytial virus pneumonia. | 1989 | 2584435 | |
| respiratory syncytial virus: a nursing perspective. | respiratory syncytial virus (rsv), a common winter illness, is most devastating in infants with underlying pulmonary and cardiovascular disease. ribavirin aerosol is an effective treatment when used with appropriate nursing management. | 1989 | 2587088 |
| [assessment of potential epidemiologic danger of sources of respiratory syncytial infection by biological properties of the pathogen]. | biological properties of 188 strains of respiratory syncytial (rs) virus isolated from 93 institutionalized children during 4 years of observations were correlated with the form and stage of the infectious process, frequency of ard, and blood group of the children for the evaluation of the epidemiological hazard of sources of rs infection. the degree of virulence of a strain was evaluated by its yields in human embryo lung culture and sensitivity to antibodies (avidity). in clinically manifest f ... | 1989 | 2588555 |
| detection of multiple viral agents in nasopharyngeal specimens yielding respiratory syncytial virus (rsv). an assessment of diagnostic strategy and clinical significance. | a retrospective study of 6 years (1981-1987) experience with clinical specimens of pediatric patients submitted for identification of respiratory viruses was undertaken to determine the prevalence of multiple viral isolates and to assess the impact of dual infections on severity of clinical disease. respiratory syncytial virus (rsv), the most frequently identified agent, was detected in cell culture and/or by immunofluorescence (if) in 666 of 2,415 specimens examined. a second virus was isolated ... | 1989 | 2591168 |
| hla class i antigens in severe rsv bronchiolitis. | cytotoxic t-lymphocytes (ctl) recognize virus-infected cells in association with hla class i antigens. there is strong evidence of the importance of ctl in respiratory syncytial virus (rsv) infections. we looked for but were unable to demonstrate an association between particular hla class i antigens and severe rsv bronchiolitis in infants. | 1989 | 2595727 |
| morbillivirus infection of seals (phoca vitulina) during the 1988 epidemic in the bay of heligoland. i. mode, frequency and significance of cultural virus isolation and neutralizing antibody detection. | from 16 (14%) out of 112 dead or euthanized seals originating from wildlife and seal orphanages phocine morbillivirus was isolated. the majority of viral isolates in cell cultures was obtained from lung homogenates of 15 out of 71 free-ranging seals (21%). the virus was isolated by longterm cultivation in roller cultures of seal kidney cells. the phocine morbillivirus was detected by typical cytopathogenic alteration and by peroxidase-linked antibody (pla) assay, respectively. a neutralization t ... | 1989 | 2596201 |
| a severe outbreak of respiratory tract disease associated with bovine respiratory syncytial virus probably enhanced by vaccination with modified live vaccine. | a severe outbreak of respiratory tract disease associated with bovine respiratory syncytial virus (brsv) on a large beef-fattening farm is described. the outbreak started two days after five- to seven-month-old calves were vaccinated with a modified live brsv vaccine. the disease ran a very severe course among five- to seven-month-old vaccinated calves, but disease was absent in eight-month-old an older non-vaccinated calves. the presence of igm antibodies in sera of non-vaccinated calves indica ... | 1989 | 2603358 |
| [severe respiratory syncytial virus infections. study of 87 infants hospitalized in an epidemic]. | during a winter epidemic, 87 infants were admitted to necker-enfants-malades hospital with a severe respiratory syncitial virus (rsv) infection. these infants fell into two groups: 37 infants without any medical history and 50 showing an underlying pathology (immune deficiencies, heart disease, cns disorders, digestive malformations, allergic manifestations). of the 37 infants with no medical history, most were below the age of 6 months and the rsv infection was manifested clinically by bronchio ... | 1989 | 2604509 |
| ribavirin's risks in reproduction--how great are they? | 1989 | 2607924 | |
| morbillivirus infection among seals (phoca vitulina) during the 1988 epidemic in the bay of heligoland. ii. serogological investigations reflecting a previous phocine distemper epidemic in a seal orphanage. | of 57 seals hospitalized in the norddeich orphanage, 37 (65%) had died until 22nd of august, 1988, when the first collection of blood samples from the survivors commenced. all the sera including those obtained at a later date from the remaining animals had invariably higher neutralizing antibody titres against a phocine distemper virus (pdv) isolate than against canine distemper virus (cdv). the difference of mean titres was calculated to be more than 1.5 x log10 serum dilution. peak titres demo ... | 1989 | 2609807 |
| [the immunization of adults against respiratory syncytial virus infection]. | the results of the comparative study of the immunological effectiveness of experimental samples of respiratory syncytial (rs) viral vaccine, prepared from a live attenuated strain and introduced in a single administration to young adults by the intranasal, intradermal and combined intranasal-intradermal) routes, are presented. the effectiveness of intranasal immunization was inversely related to the level of previously existing humoral (serum, secretory) antibodies. intradermal immunization enha ... | 1989 | 2609811 |
| [virologic and serologic observations in an outbreak of infantile bronchopneumonitis]. | from december 1985 to february 1986 in si hong county, jiang su province an outbreak of bronchopneumonitis occurred. in december the number of admitted infant cases with bronchopneumonitis was 32% of total hospitalized cases. the youngest infant was 28 days in age and the oldest was one and half years. 71.5% percent occurred in children less than six months in age. from nasopharyngeal washing of acute cases were isolated 6 strains of viruses. the result of identification of them revealed that th ... | 1989 | 2611868 |
| identification of the antigenic components of paramyxovirus-3, paramyxovirus-6 and newcastle disease virus in turkeys. | the aim of this study was to use the enzyme-linked immunosorbent assay (elisa) and the western immunoblotting as possible tools to differentiate infections in turkeys by different paramyxoviruses. pooled hyperimmune sera of turkeys infected with either paramyxovirus-3 (pmv-3), paramyxovirus-6 (pmv-6), or newcastle disease virus (ndv) were assayed for antibodies specific to the three viruses by the elisa and western immunoblotting. elisa results showed cross reactions of turkey antibodies between ... | 1989 | 2617849 |
| interferon alfa, infectious virus, and virus antigen secretion in respiratory syncytial virus infections of graded severity. | interferon alfa was measured by an immunoradiometric assay in the nasopharyngeal secretions of a group of infants admitted to hospital with respiratory syncytial virus infection. virus replication in the upper respiratory tract was assessed by infectivity assay and by an enzyme linked immunoadsorbent assay for the viral fusion protein on the same nasopharyngeal secretions. all infants were examined daily while in hospital and allocated a score based upon a subjective assessment of the severity o ... | 1989 | 2624471 |
| igg and igm antibodies to viral glycoproteins in respiratory syncytial virus infections of graded severity. | serum antibodies to the fusion (f) and large glycoprotein (g) of respiratory syncytial virus in the serum of 57 infected infants were measured by enzyme linked immunosorbent assay (elisa). most serum samples taken at the time of admission to hospital contained antibodies to both glycoproteins, and overall there was no significant evidence of a selective deficiency of antibody to either viral antigen. less than a quarter of the infants showed rising igg antibody titres to either glycoprotein afte ... | 1989 | 2624472 |
| [respiratory syncytial virus antibody levels in the 13-25 age group of healthy people using a microneutralization test]. | in this study antibody levels to respiratory syncytial virus were investigated in 181 serum samples obtained from healthy individuals by using microneutralization test. 25 out of 181 sera (13.8%) were found positive at 1/5 dilution. serum neutralization50 (sn50) value of these sera were detected at different titers which varied from 1/7.08 to 1/79.5. | 1989 | 2626101 |
| immunization of cotton rats with the human respiratory syncytial virus f glycoprotein produced using a baculovirus vector. | the f glycoprotein of respiratory syncytial virus in insect cells was produced using a baculovirus expression vector to examine its potential as a subunit vaccine. two different forms of the f glycoprotein were expressed: the intact f (f) glycoprotein and the truncated (ft) glycoprotein, in which the cooh-terminal anchor region was deleted. the f glycoprotein remained cell associated, whereas the ft glycoprotein was secreted into the media of infected cells. in contrast to the processing of the ... | 1989 | 2644369 |
| comparison of the anti-respiratory syncytial virus activity and toxicity of papaverine hydrochloride and pyrazofurin in vitro and in vivo. | based on reports describing their broad antiviral activity, the toxicity and antiviral efficacy of papaverine hydrochloride and pyrazofurin against respiratory syncytial virus (rsv) infection were tested in vitro in tissue culture cells and in vivo in cotton rats. papaverine inhibited rsv replication in vitro; however, the median minimal toxic dose-median minimal inhibitory concentration ratios (mtd50:mic50) in vitro and in vivo for papaverine were less than 4. further work with this compound wa ... | 1989 | 2653219 |
| morbillivirus in seals: isolation and some growth characteristics in cell cultures. | the procedure used for the cultural isolation of morbillivirus from seals as well as some growth characteristics of isolates is described briefly. emphasis was laid upon cytopathic changes typical for morbillivirus and observed in seal kidney cell culture subsequent to inoculation of organ tissue suspensions or buffy coat leucocytes. the modalities which resulted in the identification of 21 morbillivirus isolates from 16 seals by using direct fluorescent antibody (fa) or peroxidase linked antibo ... | 1989 | 2653775 |
| [atypical interstitial pneumonia (aip) in calves and young cattle in schleswig-holstein in conjunction with an infection by the bovine respiratory syncytial virus (brsv)]. | it is reported on atypical interstitial pneumonia (aip) in 16 mostly holstein-frisian calves and feeders from 13 different farms in schleswig-holstein in association with an infection by the respiratory syncytial virus (brsv). all animals were submitted with identical clinical histories. macroscopically the lung lesions were characterized by alveolar and interstitial edema and emphysema. microscopically there was a diffuse interstitial pneumonia with formation of hyaline membranes and multinucle ... | 1989 | 2653786 |
| use of high-speed rolling to detect respiratory syncytial virus in cell culture. | we examined the effect of motion on respiratory syncytial virus (rsv) growth in cell culture. infected cultures were incubated stationary, rolling, or on an orbital shaker. enzyme immunoassay (eia) results for cultures infected with high concentrations of a laboratory strain of rsv were similar for all incubation conditions. however, cultures infected with low concentrations of virus and rolled at 96 rpm had a significantly greater mean eia optical density (1.78 +/- 0.22) than cultures rolled at ... | 1989 | 2654185 |
| respiratory syncytial virus alters electrophysiologic properties in cotton rat airway epithelium. | the effect of respiratory syncytial virus (rsv) infection on the electrophysiologic properties of the airway epithelium was studied in tracheas obtained from cotton rats, after in vivo exposure to the virus. rsv infection was documented by tissue culture infectivity and immunofluorescent antibody techniques. light microscopic studies of the tracheas 72 hours after exposure to rsv revealed normal epithelial morphology. rsv infection produced a 28% decrease in short-circuit current (isc) and an al ... | 1989 | 2654848 |
| mechanism of lung injury in cotton rats immunized with formalin-inactivated respiratory syncytial virus. | respiratory syncytial virus (rsv) seronegative cotton rats were immunized intramuscularly at four and eight weeks of age with either formalin-inactivated, alum-precipitated rsv grown in hep-2 cell tissue cultures or virus-free hep-2 cell tissue cultures similarly prepared. sham-immunized animals served as controls. at 12 weeks of age, all animals were challenged with 6 x 10(5) plaque forming units of live rsv via the intranasal route. animals were killed at predetermined days and evaluated for r ... | 1989 | 2655343 |
| evaluation of the abbott testpack rsv enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens. | the abbott testpack rsv assay (abbott laboratories, north chicago, ill.), a rapid (20-min) enzyme immunoassay, was compared with culture and direct immunofluorescence (dfa) of nasopharyngeal cells for the detection of respiratory syncytial virus (rsv) in nasopharyngeal swab specimens. nasopharyngeal swab specimens, collected from 234 infants, were placed in viral transport medium. portions of specimen in transport medium were used for each test. of 234 specimens, 70 (30%) were culture positive, ... | 1989 | 2666434 |
| development of cell-mediated cytotoxic immunity to respiratory syncytial virus in human infants following naturally acquired infection. | with virus-infected autologous and allogenic mononuclear cells as specific targets, the development of cell-mediated cytotoxic reactivity to respiratory syncytial virus (rsv) was studied in peripheral blood lymphocytes (pbl) in groups of infants with acute rsv infection and in other control groups of subjects during a community outbreak of rsv infection. no rsv-specific cellular cytotoxicity was observed in cord blood lymphocytes and in other uninfected controls. the pbl of infants with acute rs ... | 1989 | 2666570 |
| [the occurrence of paramyxoviruses in game birds: epizootiological aspects, characteristics in vivo and in vitro. a review of the literature]. | the recent review is based on recent literature on the epizootiology and on properties of avian paramyxoviruses in vivo and in vitro. paramyxoviruses are classified according to their pathogenicity (especially serotype 1), in addition to serotyping by means of hemagglutination-inhibition tests and by monoclonal antibody binding assays. also, neuraminidase-inhibition tests, double-immunodiffusion tests can be performed. growth properties of the different paramyxovirus-serotypes are accessory tech ... | 1989 | 2669426 |
| antigenic characterization of respiratory syncytial virus associated with acute respiratory infections in uruguayan children from 1985 to 1987. | the occurrence of subgroup a and b strains of respiratory syncytial virus (rsv) was studied during three epidemic years, 1985 to 1987, in uruguay. a set of monoclonal antibodies was selected according to their reactivity with local rsv isolates and used for the typing of rsv directly in nasopharyngeal cells by indirect immunofluorescence. of 77 specimens, 69 could be typed as belonging to subgroup a or b, 5 could not be typed with the restricted set of monoclonal antibodies employed, and 3 react ... | 1989 | 2671013 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by dna-rna hybridization. | we have developed an rna-cdna hybridization assay for the detection of respiratory syncytial virus (rsv) rna in nasopharyngeal samples. we chose to use as probe a cdna complementary to the nucleocapsid protein gene of rsv, integrated into the plasmid vector pbr322. the lower limit of sensitivity of the assay is 8.2 x 10(2) pfu of the long strain of rsv. in throat washes with added cell-free virus, the assay can detect 3.3 x 10(3) pfu of rsv. respiratory secretions were collected from a group of ... | 1989 | 2671029 |
| immunosuppression of the antibody response to respiratory syncytial virus (rsv) by pre-existing serum antibodies: partial prevention by topical infection of the respiratory tract with vaccinia virus-rsv recombinants. | immunization strategies to prevent respiratory syncytial virus (rsv) disease will involve immunization of infants less than 2 months of age who possess maternally derived rsv antibodies. vaccinia-rsv recombinant viruses are useful tools for defining parameters important in immunization against rsv and also are being considered as live virus vaccines for use in humans. previous studies demonstrated that passively acquired rsv antibodies can suppress the immune response and the protective efficacy ... | 1989 | 2671259 |
| rapid detection of respiratory syncytial virus in nasopharyngeal secretions by immunofluorescence and elisa does not justify discontinuation of virus isolation. | direct fluorescent antibody assay (dfa) using monoclonal antibody and enzyme-linked immunosorbent assay (elisa) for rapid detection of respiratory syncytial virus (rsv) in nasopharyngeal secretions (nps) were compared with conventional virus isolation and identification procedures in cell cultures. when 134 nps were examined from infants and young children with acute respiratory tract infection, 42 (31%) were culture-positive for rsv and 31 of these were detected by the appearance of a typical c ... | 1989 | 2674634 |
| protection of cotton rats against human respiratory syncytial virus by vaccination with a novel chimeric fg glycoprotein. | the cotton rat model of experimental human respiratory syncytial virus (rsv) infection was used to study the efficacy of fg, a novel chimeric glycoprotein which was expressed in insect cells using a baculovirus vector. fg contained the extracellular regions of the f (fusion) and g (attachment) glycoproteins of rsv. vaccination with fg resulted in induction of neutralizing antibody and was correlated with protection of lung tissue from rsv challenge against both serogroup a and b virus strains. b ... | 1989 | 2677234 |
| respiratory syncytial virus-induced acute lung injury in adult patients with bone marrow transplants: a clinical approach and review of the literature. | acute lung injury induced by respiratory syncytial virus (rsv) is a major cause of morbidity and mortality in patients who have undergone bone marrow transplantation. twenty-nine of the 74 patients who received bone marrow transplants at the university of minnesota during a 1-year period developed evidence of acute lung injury, and rsv was identified as the cause in 8. we discuss the clinical course of these 8 patients and offer a clinical approach to rsv infection occurring after bone marrow tr ... | 1989 | 2677595 |
| comparison of nasal brush and nasopharyngeal aspirate techniques in obtaining specimens for detection of respiratory syncytial viral antigen by immunofluorescence. | diagnosis of respiratory syncytial virus by antigen detection is dependent on obtaining adequate respiratory epithelial cells. two specimen collection methods, nasopharyngeal aspiration (npa) and nasal brushing (nb), were compared. thirty-two pediatric patients with presumed viral pneumonia or bronchiolitis (34 episodes) had both npa and nb performed. of 34 specimens 15 were culture-positive for respiratory syncytial virus. of these 12 npa samples and 10 nb samples had viral inclusions by immuno ... | 1989 | 2677955 |
| [outbreak of respiratory syncytial virus in a neonatal unit]. | described is an outbreak of nosocomial rsv infection, following the admission of a 20-day-old and 11-day-old newborn with lower respiratory infections, diagnosed as being caused by rsv through detection of viral antigens by direct fat (fluorescent antibody test) of nasopharyngeal samples. later on, 5 of the other 20 newborn in patients in the same care unit also showed positive results in direct fat, for rsv. the clinical manifestations of these patients were mild even in those cases with underl ... | 1989 | 2679277 |
| how do vaccinia-vectored vaccines fit into animal immunization programmes? | 1989 | 2685957 | |
| [immunologic assessment of preparations of the rs virus at the stages of its purification and concentration]. | the work demonstrates the immunogenic potency of some preparations of rs virus, differing in the degree of their purification and introduced by multiple intranasal administration. the immunogenic potency of these preparations was manifested by the synthesis of antibodies determined in the coagglutination test (the specificity of these antibodies was confirmed in the reaction of the neutralization of rs virus), as well as by sensitization determined in the leukocyte migration inhibition test. imm ... | 1989 | 2686314 |
| epidemiology and control of nosocomial viral infections. | the impact of nosocomial viral disease, in terms of morbidity, mortality, and health care expenditures, should not be underestimated. respiratory viruses, in particular, account for a substantial proportion of all nosocomial infections, especially among pediatric patients and the institutionalized elderly. the immunocompromised, very young, and chronically ill patients in hospitals are unusually vulnerable to serious viral illness. the emerging technology of rapid viral diagnosis will allow more ... | 1989 | 2687364 |
| the paramyxoviridae: aspects of molecular structure, pathogenesis, and immunity. | 1989 | 2690597 | |
| immunization of cotton rats with the fusion (f) and large (g) glycoproteins of respiratory syncytial virus (rsv) protects against rsv challenge without potentiating rsv disease. | a formalin-inactivated respiratory syncytial virus (rsv) vaccine tested 22 years ago failed to protect infant vaccinees against rsv infection or disease. instead, lower respiratory tract disease was enhanced during subsequent infection by rsv. enhancement of pulmonary pathology is also observed when cotton rats are immunized with formalin-inactivated rsv and subsequently infected with this virus. a major question that must be addressed for each new paramyxovirus vaccine is whether the immunogen ... | 1989 | 2692334 |
| antiviral factors in human milk: implications in respiratory syncytial virus infection. | 1989 | 2692385 | |
| different morbilliviruses in european and siberian seals. | 1989 | 2694584 | |
| the immunobiology of respiratory syncytial virus: prospects for a vaccine. | 1989 | 2694814 | |
| rapid detection of respiratory syncytial virus by a biotin-enhanced immunoassay: test performance by laboratory technologists and housestaff. | a biotin-enhanced enzyme immunoassay (eia) for respiratory syncytial virus (rsv) antigen detection (testpack rsv) was prospectively compared with virus isolation in cell culture and immunofluorescence. of 156 nasopharyngeal swab specimens from infants with respiratory symptoms, 81 (52%) yielded rsv in culture. compared with culture the sensitivity of the eia was 95% and specificity was 92%; the specificity increased to 97% with a blocking assay. compared with immunofluorescence the sensitivity o ... | 1989 | 2696927 |
| nosocomial viral respiratory infections. | nosocomial infections with respiratory tract viruses, particularly influenza and respiratory syncytial viruses, account for the majority of serious nosocomial viral disease. chronically ill, immunocompromised, elderly, and very young hosts are especially vulnerable to potentially life-threatening involvement of the lower respiratory tract. effective preventive strategies are based upon early accurate viral diagnosis and an appreciation of the epidemiology and mechanisms of transmission for each ... | 1989 | 2697050 |
| ribavirin in respiratory syncytial virus infection. | 1989 | 2705808 | |
| serum immunoglobulin g antibody subclass response to respiratory syncytial virus f and g glycoproteins after first, second, and third infections. | serum samples from 31 children who experienced two or three infections with respiratory syncytial virus (rsv) in the first four years of life were tested in an enzyme-linked immunosorbent assay to examine the immunoglobulin g (igg) subclass responses to the rsv f and g surface glycoproteins associated with primary infection and reinfection. we sought to determine whether the greater degree of glycosylation of the g glycoprotein was reflected in an igg subclass immune response more like that to a ... | 1989 | 2715331 |
| characterization of prolonged apneic episodes associated with respiratory syncytial virus infection. | we used high-speed polygraphic recordings to document in detail the sequence of events during prolonged apneic spells in two infants with respiratory syncytial viral (rsv) infection. also, we elicited upper airway reflexes by stimulating the airway with saline during sleep. spontaneous prolonged apneic spells were recorded in both infants, and these consisted of two distinctly different apnea types. a number of similarities between apnea of prematurity, upper airway reflex apnea and the apneas i ... | 1989 | 2717245 |
| detection of ige antibodies to bovine respiratory syncytial virus. | the role of ige antibodies against respiratory syncytial virus has attracted attention for both human and bovine disease. to detect such antibodies, we have developed an enzyme-linked immunosorbent assay (elisa) specific for bovine respiratory syncytial virus (brsv). firstly, antiserum strongly positive for brsv-specific ige was produced by immunizing a levamisole-treated calf with brsv. the presence and specificity of brsv-specific ige in this animal was confirmed with the praunitz-kustner (pk) ... | 1989 | 2718372 |
| [apnea attacks in infants: study of 7 cases]. | authors study seven young infants suffering from apneic spells, without clear origin. diagnosis was gastroesophageal reflux in the two most torpid evolution: others had hyponatremia. rvs infection, inborn cytomegaly and prematurity apnea. | 1989 | 2719413 |
| rna fingerprinting of respiratory syncytial virus using ribonuclease protection. application to molecular epidemiology. | we have used the technique of ribonuclease protection to define genomic variation among circulating isolates of subgroup a respiratory syncytial (rs) virus. rnas extracted from hep-2 cells infected with strains to be analyzed were hybridized with a 32p-labeled rna probe corresponding to the rs virus g glycoprotein (a2 strain). areas of nonhomology were detected by cleavage with ribonuclease a. using this technique, multiple distinct rna cleavage patterns could be distinguished among viral isolat ... | 1989 | 2723064 |
| immunoglobulin-class-specific immune response to respiratory syncytial virus structural proteins in infants, children, and adults. | the protein specificities of igg, igm, and iga antibodies induced during respiratory syncytial virus (rsv) infection in 74 patients (4 weeks to 81 years of age) were investigated using the technique of immunoblotting. although the pattern of antibody reactivity varied among patients, most of the humoral immune response in all age groups was directed against the 48, 42, 35, and 27 k proteins. an infant's own antibody response was discernible in 55 of the 57 children below 1 year of age, despite t ... | 1989 | 2723614 |
| pathogenesis of naturally acquired bovine respiratory syncytial virus infection in calves: morphologic and serologic findings. | lesions in 32 calves that died or were euthanatized during the course of severe natural infection with bovine respiratory syncytial virus (brsv) are described. all calves had been dyspneic for 1 to 2 days. at necropsy, lesions that could be related to dyspnea included congested and cyanotic mucosae and widespread petechiae. the lungs had various lesions in the cranioventral (cv) and caudodorsal (cd) portions. the cv portion of the lungs was consolidated, firm, and edematous. histologically, the ... | 1989 | 2729714 |
| immune response to experimentally induced infection with respiratory syncytial virus: possible role in the development of pulmonary disease. | groups of mice were immunized intraperitoneally with hep-2 lysate or respiratory syncytial virus (rsv)-infected hep-2 cells with or without adjuvant(s). the animals were subsequently challenged intranasally (i.n.) with rsv and/or hep-2 lysate and studied for rsv shedding in the lung, antigen-specific antibody response and pulmonary histopathology. a significant decrease in virus shedding was detected in each of four groups of animals immunized with a virus preparation and in two of four groups t ... | 1989 | 2732693 |
| analysis of the antibody response to bovine respiratory syncytial virus proteins in calves. | the antibody response in calves to natural infections with bovine respiratory syncytial virus (brsv) was analysed by radioimmunoprecipitation assays. antibodies to virus proteins of mr 200k (l), 87k (g), 46k (f1), 41k (n), 35k (p), 28k and 24k (f2), 27k (m), 22k and less than 14k could be identified. recovery of 6- to 7-month-old calves from severe brsv-associated disease was accompanied by the development of an antibody response to the virus, which was directed mainly o the f and n proteins. ca ... | 1989 | 2732702 |
| [paget's bone disease. epidemiology and etiology]. | paget's bone disease is a chronic and usually benign bone disorder mainly found in people over fifty. the condition is characterized by excessive bone remodelling related to bone cell overactivity leading to abnormal bone structure. the viral origin of the disease, first proposed on the basis of ultrastructural observations, is now supported by immunocytological and molecular hybridization studies. genetic and environmental elements may be involved, favouring and modulating the expressivity of t ... | 1989 | 2734575 |
| viral and mycoplasma pneumoniae pneumonias in school-age children: three-year follow-up of respiratory function. | we studied the evolution of respiratory function during and for 3 years after the acute onset of viral and mycoplasma pneumoniae pneumonias in 13 school-age children. a mixed type transient ventilatory defect (restrictive and obstructive, but mainly restrictive) with large and small airway involvement was observed during the acute phase of the pneumonias. residual small airway involvement was found over the next 12 months, but no pulmonary function abnormalities were present after 3 years. at th ... | 1989 | 2748218 |
| bronchial reactivity in infants in acute respiratory failure with viral bronchiolitis. | airway reactivity and the effects of bronchodilators in infants are controversial. we studied the response to bronchodilator treatment in 14 mechanically ventilated infants (mean age, 2.74 months; range, 0.6-5.9) in respiratory failure caused by respiratory syncytial virus (rsv)-associated bronchiolitis. sixteen infants without lung disease, undergoing elective surgery, provided normal values. maximum expiratory deflation flow-volume (dfv) curves were produced by manual inflation of the lungs wi ... | 1989 | 2748221 |
| [respiratory insufficiency in acute bronchiolitis in infancy]. | forty-one infants with acute viral bronchiolitis were hospitalized in our paediatric intensive care unit during the seven year period from 1980 to 1987. in 14 out of 27 evaluated patients, respiratory syncitial virus (rsv) was detected in the nasal secretions. twenty-three children required only supportive care and monitoring. eighteen infants had to be ventilated because of respiratory failure. the major indication for mechanical ventilation was an arterial or capillary pco2 of more than 64 mmh ... | 1989 | 2755928 |
| prevalence of respiratory syncytial virus subgroups over six consecutive outbreaks: 1981-1987. | indirect immunofluorescence with strain-specific monoclonal antibodies was used to determine the phenotype of respiratory syncytial virus (rsv) isolates obtained from infants hospitalized in greater boston over six successive outbreaks from 1981 to 1987. of 981 isolates, 591 (60%) were classified as subgroup a and 383 (39%) as subgroup b. the prevalence of subgroups varied both between and within yearly outbreaks. in 1983-84 and 1984-85, both subgroups circulated concurrently and in almost equal ... | 1989 | 2760479 |
| [comparative studies of paramyxovirus isolates from wild birds]. | as a result of a surveillance programme in north-germany, paramyxovirus-isolates of serogroup 1 with different pathogenicity were isolated from different species of feral birds (black-headed gull, mallard, tawny owl, tree sparrow, mute swan). in chick embryo fibroblast monolayers these isolates produced plaques exhibiting different plaque morphology. after cloning the isolates, further physicochemical, serological and pathogenicity testings were performed. on the basis of mean death time and int ... | 1989 | 2763760 |
| the detection of bovine respiratory syncytial virus in formalin fixed bovine lung with commercially available monoclonal antibodies and avidin biotin complex immunohistochemistry. | eight commercially available monoclonal antibodies directed against respiratory syncytial virus antigens were tested for ability to detect bovine respiratory syncytial virus (brsv) antigen in formalin fixed, paraffin embedded bovine lung using avidin-biotin complex immunohistochemical staining. monoclonal antibodies from clone 18b2 purchased from biosoft, paris, france and those from clone 8g12 purchased from the department of veterinary sciences, university of nebraska, lincoln, nebraska staine ... | 1989 | 2766160 |
| use of nasal and pharyngeal swabs for rapid detection of respiratory syncytial virus and adenovirus antigens by enzyme-linked immunosorbent assay. | nasal and pharyngeal swabs from 134 children with acute respiratory diseases were examined for the presence of respiratory syncytial (rs) virus and adenovirus antigens by enzyme-linked immunosorbent assay (elisa). the results were compared with those obtained by virus isolation and serology. altogether, 56 rs virus-positive (prospective study), 51 adenovirus-positive (retrospective study), and 27 negative (prospective study) samples were examined. the sensitivities of elisa were 96 and 98% for r ... | 1989 | 2768473 |