Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| maridi haemorrhgic fever: a new viral disease (author's transl). | a new viral disease (maridi haemorrhagic fever) occurred in the south sudan in 1976. it was obviously identical with an epidemic which occurred at the same time in zaire. the virus is morpologically closely similar to the marburg virus. during the maridi epemic 124 of 238 patients died (52%). characteristic symptoms were fever and headache (100%), diarrhoea (83%), retrosternal pain (82%), vomiting (68%), haemorrhages (62%), morbilliform or vesicular rash (52%). at post-mortem there were changes ... | 1977 | 21783 |
| isolation and partial characterisation of a new virus causing acute haemorrhagic fever in zaire. | 1977 | 65661 | |
| viral haemorrhagic fever in southern sudan and northern zaire. preliminary studies on the aetiological agent. | 1977 | 65662 | |
| isolation of marburg-like virus from a case of haemorrhagic fever in zaire. | 1977 | 65663 | |
| after marburg, ebola. | 1977 | 65668 | |
| decreasing any viral infectivity in blood-smears for malarial parasite examination. | 1978 | 74655 | |
| ebola and marburg viruses: i. some ultrastructural differences between strains when grown in vero cells. | a strain of marburg virus and two strains of ebola virus grown in vero cells were compared by electron microscopy. the outer coat of the marburg virion appeared to be more resistant to erosion by negative staining techniques than that of the epbola strains. marburg virus commonly produced "torus" forms and short filaments; the zaire strain of ebola produced extensive branched forms and very long filaments; the sudan strain of ebola produced shorter, less branched structures but very many aberran ... | 1979 | 94087 |
| the nature and classification of viruses of man. | 1979 | 111266 | |
| ebola virus and hemorrhagic fever: andromeda strain or localized pathogen? | 1979 | 111590 | |
| ebola and marburg viruses: ii. thier development within vero cells and the extra-cellular formation of branched and torus forms. | the development of marburg virus and the sudanese and zaire strains of ebola virus in vero cells as visualized by electron microscopy is described. despite differences in timing, all three strains appear to pass through identical stages of development. initially there is a large increase in nucleolus material, and viral precursor material arranges itself in spirals and then into tubes. the cells fill with core material, which passes to the plasmalemma, which often proliferates. each virion passe ... | 1979 | 119829 |
| [infections by marburg and ebola viruses: guide for their diagnosis, treatment and control]. | 1978 | 150845 | |
| [viral contamination in laboratories and hospital units]. | viral contamination is at least as important in hospital laboratories and wards as contamination by bacteria or microscopic fungi, but it is much more insidious and sometimes unrecognized. there are two main types: the first has a purely technical effect and only interest the virologist. this is contamination of reagents, reference strains, cell cultures, etc.., by foreign viral agents. it may be the cause of errors on diagnosis or regrettable errors of interpretation of certain experimental dat ... | 1975 | 165448 |
| biohazards and simian viruses. | nonhuman primates are extensively used in laboratories as experimental animals. it is necessary, however, to realize that their employment may be dangerous to man and other species of primates, if recognition of their flora and fauna, especially viral, is not considered and if appropriate controls are not followed. several outbreaks have occurred, resulting in high mortality and morbidity of man and simian. a number of recommendations are provided which, if followed, will minimize the waste of t ... | 1975 | 169837 |
| international arbovirus research. | international cooperation in arbovirus research became increasingly prominent shortly after it was found in the early 1940's, that antigenic relationships existed among certain viruses isolated in different areas of thw world. the interest of a number of scientists and of a private organization led to the establishment of an international information exchange and to the preparation of an international catalogue of arboviruses including certain other viruses of vertebrates. international cooperat ... | 1975 | 173936 |
| early detection of antigen and estimation of virus yield in specimens from patients with marburg virus disease. | autopsy specimens from patients with marburg disease having at least 10(4.5) tcid(50) of virus per gram of tissue were found to contain sufficient fluorescent antigen-positive cells to make a specific diagnosis possible in less than 3 h. liver, heart, spleen, and kidney tissues were found to contain significant amounts of virus. tissue suspensions, as well as blood or serum samples, inoculated into vero cell cultures produced virus-specific immunofluorescence within 2-5 days. at least one specim ... | 1978 | 310364 |
| [marburg, lassa and ebola virus as cause of hemorrhagic fever]. | 1978 | 352653 | |
| [the marburg virus group]. | 1977 | 406580 | |
| [acute fever caused by marburg virus]. | 1977 | 413103 | |
| [lassa fever and marburg disease]. | 1978 | 417467 | |
| uveal involvement in marburg virus disease. | the first reported case of uveal involvement in marburg virus disease is described. | 1977 | 557985 |
| a consideration of the diagnosis of dangerous infectious fevers in south africa. | 1978 | 565951 | |
| [new high-risk infectious diseases]. | 1978 | 566498 | |
| [recent lassa, marbourg and ebola viruses in african tropical viruses. i. semiology--physiopathology--diagnosis--treatment (author's transl)]. | three new viruses have been identified in africa during the present decade. they may cause sporadic cases or limited outbreaks, and they are probably endemic in areas which are still ill-defined. severe forms of infection lead to the haemorrhagic syndrome or to hypovolemic shock, the physiopathology of which is being studied. the case-fatality ratio of severe cases is between 30 and 85 per cent. nosocomial outbreaks have been observed, but they can be avoided if appropriate barrier nursing measu ... | 1978 | 569288 |
| [viral haemorrhagic fever]. | two outbreaks of haemorrhagic fever caused by a marburg-like virus occurred in the sudan and zaire from august to november 1976. the inital epidemiological investigations and emergency control measures were carried out by sudanese and zairian health personnel and several of them unfortunately died in the course of their duties. in view of the unusual gravity of the outbreaks and the unknown nature of the agent, assistance was provided by a who team in the sudan and a multinational team in zaire. ... | 1977 | 590991 |
| ultrastructure of ebola virus particles in human liver. | electron microscopy of tissues from two necropsies carried out in the sudan on patients with ebola virus infection identified virus particles in lung and spleen, but the main concentrations of ebola particles were seen in liver sections. viral precursor proteins and cores were found in functional liver cells, often aligned in membrane-bound aggregations. complete virions, usually found only extracellularly, were mainly seen as long tubular forms, some without cores. many tubular forms had 'enlar ... | 1978 | 641193 |
| outbreake of marburg virus disease in johannesburg. | the first recognised outbreak of marburg virus disease in africa, and the first since the original epidemic in west germany and yugoslavia in 1967, occurred in south africa in february 1975. the primary case was in a young australian man , who was admitted to the johannesburg hospital after having toured rhodesia. two secondary cases occurred, one being in the first patient's travelling companion, and the other in a nurse. features of the illness included high fever, myalgia, vomiting and diarrh ... | 1975 | 811315 |
| marburg virus. | marburg virus disease, which produced 20 per cent mortality when it first occured during 1967 in germany and yugoslavia, recently appeared again in south africa. the source of the first outbreak was monkeys shipped from africa; the origin of the second episode is unclear. because distribution of the virus in nature is unknown, its threat to man cannot be readily determined. differential laboratory diagnoses of hemorrhagic fevers should be encouraged in order to learn more about the epidemiology ... | 1976 | 829039 |
| [the immunogenic properties of marburg virus proteins]. | immunogenic and protective properties of vp40 and np proteins of marburg virus were studied. vp40 protein was shown to have insignificant immunogenicity and np protein to be capable of protecting the animals from lethal infection by stimulation of cell-mediated immunity. no significant increase in the specific antibody level was found. | 1992 | 1413715 |
| carbohydrate structure of marburg virus glycoprotein. | marburg virus was propagated in e6 cells, a cloned cell line of vero cells, in the presence of [6-3h]glucosamine. radiolabelled viral glycoprotein was digested with trypsin, and oligosaccharides were liberated by sequential treatment with endo-beta-n-acetylglucosaminidase h, peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase f and o-glycosidase, by beta-elimination, and by alkaline hydrolysis. after fractionation by hplc and gel filtration, glycans were characterized chromatographically, ... | 1992 | 1421752 |
| [the effect of the methods for producing an experimental marburg virus infection on the characteristics of the course of the disease in green monkeys]. | a comparative study on the features of the pathogenesis of marburg disease after parenteral and aerosol infection of green monkeys with a virus prepared from native culture suspension and that after lyophilization was carried out. the changes in the dynamics of the clotting time, the activity of serum aminotransferases, the duration of prefebrile period and survival time were analysed in different cases. no lethality was observed in animals infected with small doses of aerosol preparations. | 1992 | 1441442 |
| sequence analysis of the marburg virus nucleoprotein gene: comparison to ebola virus and other non-segmented negative-strand rna viruses. | the first 3000 nucleotides from the 3' end of the marburg virus (mbg) genome were determined from cdna clones produced from genomic rna and mrna. identified in the sequence was a short putative leader sequence at the extreme 3' end, followed by the complete nucleoprotein (np) gene. the 5' end of the np mrna was determined as was the polyadenylation site for the np gene. the transcriptional start (3' uucuucuuauaauu..) and termination (3' ..uaauucuuuuu) signals of the mbg np gene are very similar ... | 1992 | 1538192 |
| the nucleotide sequence of the l gene of marburg virus, a filovirus: homologies with paramyxoviruses and rhabdoviruses. | the nucleotide sequence of the l gene of marburg virus, strain musoke, has been determined. the l gene has a single long open reading frame encoding a polypeptide of 2330 amino acids (mw 267,175) that represents the viral rna-dependent rna polymerase. the putative transcription start signal (3'cuaccuauaauu 5') and the termination signal (3' uaauucuuuuu 5') of the gene could be identified. computer-assisted comparison of the l protein with l proteins of other nonsegmented negative-stranded rna vi ... | 1992 | 1546452 |
| marburg virus, a filovirus: messenger rnas, gene order, and regulatory elements of the replication cycle. | the genome of marburg virus (mbg), a filovirus, is 19.1 kb in length and thus the largest one found with negative-strand rna viruses. the gene order - 3' untranslated region-np-vp35-vp40-gp-vp30-vp24-l-5' untranslated region-resembles that of other non-segmented negative-strand (nns) rna viruses. six species of polyadenylated subgenomic rnas, isolated from mbg-infected cells, are complementary to the negative-strand rna genome. they can be translated in vitro into the known structural proteins n ... | 1992 | 1626422 |
| [the detection of the marburg virus antigen by solid-phase immunoenzyme analysis]. | comparative studies of two variants of the enzyme-linked immunosorbent assay (elisa) were carried out to determine the sensitivity of the detection of marburg virus antigens in vero cells. both competitive and two-antibody elisa variants detected as little as 5 ng of marburg virus antigen. the vero cell monolayer was found to produce 5-50 ng/0.05 ml of the virus-specific proteins at 6 to 8 days postinfection. | 1991 | 1725078 |
| [a comparative study of the in-vitro interaction of the marburg virus with macrophages from different animal species]. | the amplification of marburg virus in primary cultures of peritoneal macrophages of animals with different sensitivity to infection with this virus, as well as the capacity of this virus to adsorb on macrophages were studied. macrophages of the animals sensitive to marburg virus were capable to support its reproduction in vitro whereas those of resistant animals were not. macrophages of the animals with intermediate sensitivity were shown to be either completely resistant to virus reproduction o ... | 1991 | 1785187 |
| [an experimental study of the contact transmission of the marburg virus]. | experiments in guinea pigs and m. rhesus monkeys showed the possibility of contact infection of animals with marburg virus. secondary infection occurred most intensively when the monkeys were kept together but was also shown to be possible when the animals were separated but placed in the direction of the air flow from the sick monkeys as well as by "nose-to-nose" contact excluding the alimentary mode of transmission and the role of the agent excreted in the urine. | 1991 | 1785188 |
| [a comparison of the methods of fluorescent antibodies and solid-phase immunoenzyme analysis in the detection of marburg virus antibodies]. | 1991 | 1796591 | |
| [a comparative study of the immunological indices in guinea pigs administered an inactivated marburg virus]. | samples of concentrated and unconcentrated preparations of marburg virus were studied. the ultrafiltration method helped increase the specific protein content and decrease the common protein content in a preparation. the resistance index increased with an increase of the specific protein content and the maximum (2.63 +/- 0.2) was reached by two immunizations on 0 and 14 days. | 1991 | 1803779 |
| [the sensitivity of different experimental animals to the marburg virus]. | 1991 | 1803784 | |
| [the sensitivity of different cell culture lines to the marburg virus]. | 1991 | 1803785 | |
| [marburg virus: the first determined nucleotide sequence of two genes]. | the preparations of purified marburg virus were isolated from blood plasma of infected guinea pigs and characterized. viral rna was extracted from the virions. the cdna was synthesized on the isolated rna matrix by the reverse transcriptase with the use of dissipated priming. the obtained cdna was inserted into the plasmid pbr322 by the connector technique and the resulting recombinant plasmids were cloned in escherichia coli cells. the specific clones selected by molecular hybridization method ... | 1991 | 1857371 |
| [marburg hemorrhagic fever]. | 1991 | 1882631 | |
| a single amino acid change in the e2 glycoprotein of venezuelan equine encephalitis virus affects replication and dissemination in aedes aegypti mosquitoes. | four monoclonal antibody-resistant variants (marvs) of venezuelan equine encephalitis (vee) virus were used to study mosquito-virus interactions. in vitro experiments using an aedes albopictus cell line, c6/36, demonstrated that an amino acid change in the glycoprotein e2h epitope (marv 1a3b-7) decreased virus growth when compared with the wild-type, trinidad donkey virus, and its vaccine derivative, tc-83. the marvs replicated as efficiently as the parent virus when inoculated into aedes aegypt ... | 1991 | 1919525 |
| detection of ebola-like viruses by immunofluorescence. | 1990 | 1979412 | |
| glycosylation and oligomerization of the spike protein of marburg virus. | the oligosaccharide side chains of the glycoprotein of marburg virus (mw 170,000) have been analyzed by determining their sensitivity to enzymatic degradation and their reactivity with lectins. it was found that they consist of n- and o-glycans. studies employing chemical cross-linking showed that the glycoprotein is present as a homotrimer in the viral envelope. | 1991 | 2024471 |
| antibody prevalence against haemorrhagic fever viruses in randomized representative central african populations. | between 1985 and 1987, 5,070 randomly selected persons living in 6 central african countries (cameroon, central african republic, chad, congo, equatorial guinea and gabon) were checked for serological evidence of haemorrhagic fever. rural and urban areas were studied, including ecoclimatic zones ranging from dry savana to tropical rain forest. virus-reactive antibodies were found with all antigens tested, and the global prevalence of positive sera was distributed as follows: crimean-congo haemor ... | 1989 | 2505350 |
| antibodies to haemorrhagic fever viruses in madagascar populations. | sera of 381 adult people from 5 areas in madagascar were tested by the indirect immunofluorescence method for antibodies against congo-crimean haemorrhagic fever and rift valley fever viruses (bunyaviridae), ebola (strains zaire and sudan) and marburg viruses (filoviridae), and lassa virus (arenaviridae). the highest prevalence rate was that of ebola virus (4.5%). as no haemorrhagic syndrome has been found associated with this virus, the possible presence of a less pathogenic, antigenically rela ... | 1989 | 2515626 |
| hemorrhagic fever virus infections in an isolated rainforest area of central liberia. limitations of the indirect immunofluorescence slide test for antibody screening in africa. | serum samples from 119 healthy individuals and 106 epilepsy patients inhabiting grand bassa county, liberia, were tested for antibodies to hemorrhagic fever viruses (hfv) by indirect immunofluorescence. e6 vero cells infected with lassa fever virus (las), rift valley fever virus (rvf), congo hemorrhagic fever virus (con), marburg virus (mbg) and the ebola (ebo) virus strains mayinga (may) and boniface (bon) were used as antigen. to obtain reproducible and specific test results sera had to be abs ... | 1986 | 3092415 |
| viral hemorrhagic fever antibodies in nigerian populations. | using the immunofluorescence test, a serosurvey for antibodies to five viral agents associated with hemorrhagic febrile infections was conducted with 1,677 human sera from different parts of nigeria. three hundred fifty-seven (21.3%) were positive for lassa virus antibody, while antibodies to rift valley fever virus were detected in 42 (2.5%) of the sera. testing for rift valley fever virus antibody was confirmed by plaque reduction neutralization test. antibodies to ebola and marburg viruses we ... | 1988 | 3128130 |
| physicochemical properties of marburg virus: evidence for three distinct virus strains and their relationship to ebola virus. | the physicochemical and antigenic properties of three groups of marburg (mbg) virus isolates, separated temporally and geographically, were compared to each other and to another member of the same family, ebola (ebo) virus. each mbg isolate contained seven virion proteins, one of which was a glycosylated surface protein. peptide mapping of glycoproteins, nucleoproteins (np) and viral structural protein (vp40) demonstrated extensive sequence conservation in the proteins of viruses isolated over a ... | 1988 | 3404120 |
| [method for the simultaneous detection of immunofluorescing antibodies to the causative agents of different hemorrhagic fevers]. | 1985 | 3907144 | |
| conservation of the 3' terminal nucleotide sequences of ebola and marburg virus. | the 3' rna base sequences of several marburg (mbg) and ebola (ebo) virus isolates have been determined. a comparison of these 3' terminal noncoding sequences with those of other negative strand rna viruses suggests a unique phylogenic niche for marburg and ebola viruses. the translation initiation site and 35 n-terminal amino acids of the 3' proximal coding gene of a zaire strain of ebola virus was predicted. in addition, putative leader rna sequences preceding the first gene are discussed in te ... | 1986 | 3946083 |
| [ecology of "marburgvirus" (rhabdovirus simiae)]. | 1969 | 4989351 | |
| [complement-fixing antibodies in man after infection with "marburgvirus" (rhabdovirus simiae)]. | 1969 | 5393201 | |
| viral haemorrhagic fevers: properties and prospects for treatment and prevention. | 1984 | 6091539 | |
| marburg and ebola virus antibodies in kenyan primates. | 1981 | 6113374 | |
| inactivating lassa and marburg/ebola viruses. | 1982 | 6123862 | |
| modifications to indirect immunofluorescence tests on lassa, marburg, and ebola material. | 1983 | 6131336 | |
| lack of cross reactivity of rhabdovirus antibodies with marburg and ebola antigens in the indirect immunofluorescent antibody test. | 1982 | 6179483 | |
| antibodies against haemorrhagic fever viruses in kenya populations. | human sera from lodwar (77 sera), nzoia (841 sera), masinga (251 sera), laisamis (174 sera) and the malindi/kilifi area (556 sera) in kenya were tested by indirect immunofluorescence for antibodies against marburg, ebola (zaire and sudan strains), congo haemorrhagic fever, rift valley fever and lassa viruses. antibodies against ebola virus, particularly the zaire strain, were detected in all regions and were, over-all, more abundant than antibodies against the other antigens. ebola and marburg a ... | 1983 | 6419422 |
| physicochemical inactivation of lassa, ebola, and marburg viruses and effect on clinical laboratory analyses. | clinical specimens from patients infected with lassa, ebola, or marburg virus may present a serious biohazard to laboratory workers. we have examined the effects of heat, alteration of ph, and gamma radiation on these viruses in human blood and on the electrolytes, enzymes, and coagulation factors measured in laboratory tests that are important in the care of an infected patient. heating serum at 60 degrees c for 1 h reduced high titers of these viruses to noninfectious levels without altering t ... | 1984 | 6490832 |
| antibody to ebola virus in guinea pigs: tandala, zaire. | a case-control study was conducted to investigate the findings of antibody to ebola virus in the serum of a guinea pig from tandala, zaire. case households, defined by the possession of one or more guinea pigs, were compared to neighboring households without guinea pigs. seven (5.1%) of 138 samples of human sera and 36 (26%) of 138 samples of guinea pig sera had antibody to ebola virus. there was no clustering of seropositivity among humans or guinea pigs within households, nor was there any ass ... | 1982 | 6750007 |
| viral haemorrhagic fever antibodies in zimbabwe schoolchildren. | 1982 | 6761909 | |
| [pathogenic microbiology of international communicable diseases]. | 1980 | 6771443 | |
| [marburg virus disease: epidemiology, symptomatology, management and prognosis]. | 1980 | 6771444 | |
| [antibodies against the marburg virus among human populations in the southeastern central african republic]. | a serological survey was carried out in the south-east of the central african republic in 1979 to investigate the incidence of marburg virus in the human population. serum samples were tested using the indirect immunofluorescence method on infected and inactivated vero cells (green monkey kidney) on slides prepared by the center for disease control, atlanta, u.s.a. out of 499 samples, 7 were positive. two of them showed a high antibody titre of 1/64. both patients were retested 10 months later a ... | 1981 | 6786769 |
| marburg, ebola and rift valley fever virus antibodies in east african primates. | sera from 464 primates held at four institutes in kenya were tested by indirect immunofluorescence for the presence of antibodies against marburg, ebola, congo haemorrhagic fever, rift valley fever and lassa viruses. four of 136 vervet monkeys were positive for marburg virus antibodies and three of 184 baboons had antibodies against ebola virus. one baboon was positive for marburg virus antibodies. two vervet monkeys, three baboons and one grivet monkey (of 56 tested) had antibodies against rift ... | 1982 | 6810518 |
| preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys. | a method is described for preparation of polyvalent antigens for use in rapid screening for immunofluorescent antibodies to lassa, marburg, and ebola viruses. the technique uses mixtures of specifically infected vero cells placed on teflon-templated microscopy slides. it was found to be as sensitive as the use of monovalent antigens for detection and quantitation of antibodies to these highly hazardous human pathogens. | 1981 | 7031084 |
| use of betapropionolactone inactivated ebola, marburg and lassa intracellular antigens in immunofluorescent antibody assay. | 1982 | 7049096 | |
| filoviridae: a taxonomic home for marburg and ebola viruses? | 1982 | 7118520 | |
| inactivation of lassa, marburg, and ebola viruses by gamma irradiation. | because of the cumbersome conditions experienced in a maximum containment laboratory, methods for inactivating highly pathogenic viruses were investigated. the infectivity of lassa, marburg, and ebola viruses was inactivated without altering the immunological activity after radiation with co60 gamma rays. at 4 degrees c, lassa virus was the most difficult to inactivate with a rate of 5.3 x 10(-6) log 50% tissue culture infective dose per rad of co60 radiation, as compared with 6.8 x 10(-6) log 5 ... | 1982 | 7153317 |
| haemorrhagic fever in gabon. i. incidence of lassa, ebola and marburg viruses in haut-ogooué. | a serological enquiry aimed at determining the incidence of infection with lassa, ebola and marburg viruses was conducted on the human population of the region of haut-ogooué (gabon) and on primates. the results, obtained by the indirect immunofluorescence technique, showed that more than 6% of the human population had had contact with ebola virus but no antibodies against marburg or lassa viruses were found. most sera reacted to an ebola antigen from a zairian strain, but showed little or no re ... | 1982 | 7164137 |
| [certain pathogenetic characteristics of a disease in monkeys in infected with the marburg virus by an airborne route]. | time course of marburg virus (strain popp) accumulation and changes in hematological parameters were studied in aerosol infected m.rhesus monkeys. the lungs were the first organ in which the virus was detected after respiratory infection of monkeys. four days after inoculation the virus was detected in the liver, spleen, blood, and thymus. six days after inoculation the virus was present in virtually all organs and secretions. the period of fever was associated with manifest leukopenia in primat ... | 1995 | 7483565 |
| [a promising method for preparative production and purification of the marburg virus]. | a method for the production of marburg virus in preparative amounts has been developed. it is based on sedimentation of the virus from blood plasma of infected guinea pigs by ultracentrifugation followed by purification in sucrose density gradient or gel chromatography on macroporous glass sorbents. the optimal terms of blood collection in infected animals were determined. purified virus did not lose its biological activity. concentrated virus preparations were studied by electron microscopy and ... | 1995 | 7483566 |
| the complete nucleotide sequence of the popp (1967) strain of marburg virus: a comparison with the musoke (1980) strain. | the nucleotide sequence of genomic rna of marburg virus strain popp was determined. strain popp was isolated in 1967 during the first filoviral outbreak. the virus was purified from blood of infected guinea pigs in which it had been maintained. the length of the determined sequence was 19112 nucleotides. amino acid sequences of seven known virion proteins were deduced. nucleotide and amino acid sequences were compared with those of strain musoke of marburg virus isolated in 1980 in kenya and pur ... | 1995 | 7487490 |
| [theoretical approaches to the evaluation of antiviral drug effectiveness]. | it is suggested that (a) ld50 is the measure of the efficacy of antiviral drugs: (b) a disease is characterized by the "barrier" type of a pathological process when in pathogenesis there exists an event whose manifestation largely determines the outcome of a disease (recovery/severe form with a fatal outcome). if the event consists in the penetration of a pathogen into a susceptible cell and the consequent reproduction of an antigen, the value ld50 for man is expressed as the same parameter for ... | 1993 | 7510180 |
| from the centers for disease control and prevention. update: management of patients with suspected viral hemorrhagic fever--united states. | 1995 | 7616624 | |
| [mechanisms of protective immune response in models of marburg fever in monkeys]. | the parameters of nonspecific immunity (interferon, tumor necrosis factor, natural killers), of spontaneous and mitogen-induced lymphocyte proliferation, of specific immunity (antibodies to marburg virus, antigen-stimulated proliferation) were found to change in the course of experimental marburg fever in immune and intact animals. the named factors were shown to influence the course of the disease; their role in the lethal outcome of the infection was demonstrated. | 1995 | 7676670 |
| [clinical-virusological characteristics of disease in guinea pigs, infected by the marburg virus aerogenically]. | marburg virus (strain popp) was found to accumulate in various organs of guinea pigs after aerogenic infection. at the initial stage of the infection primary multiplication of the virus took place in the lungs. the presence of the virus in bronchopulmonary washings 2-3 days after infection, leukopenia and hyperthermia 4 days after infection are the earliest virological and clinical signs of disease in aerogenically infected guinea pigs. | 1995 | 7676673 |
| virologic investigation of a case of suspected haemorrhagic fever. | after travelling in subsaharan africa, an area known for sporadic cases of marburg virus infection, a young swedish man presented with a classical picture of severe viral haemorrhagic fever complicated by disseminated intravascular coagulation and septicaemia. serum samples examined by electron microscopy revealed particles of a size compatible with filovirions. indirect fluorescent antibody tests indicated transient seroconversion to marburg virus. in lymphocyte transformation assays of cells i ... | 1994 | 7709077 |
| [marburg virus and mononuclear phagocytes: study of interactions]. | interactions of marburg virus and mononuclear phagocytes of guinea pigs were studied using a complex of virologic methods and electron microscopy. active reproduction of marburg virus in peritoneal macrophages both in vitro and in vivo was revealed. the study showed that mononuclear phagocytes were the first target cells in guinea pigs intraperitoneally infected with marburg virus which provided virus dissemination into interstitial tissue of viscera. analysis of the results indicates an importa ... | 1994 | 7716905 |
| complete nucleotide sequences of marburg virus genes 5 and 6 encoding vp30 and vp24 proteins. | nucleotide sequences of the genes 5 and 6 of the marburg virus, popp strain, were determined. orfs encoding polypeptides vp30 (281 a.a., mw 32,640) and vp24 (253 a.a., mw 28,621) were found. the putative transcription start and stop signals for viral rna-dependent rna polymerase were revealed for both genes. overlapping of genes 5 and 6 was shown. the deduced amino acid sequences of vp30 and vp24 proteins displayed significant homology with the analogous proteins of another filovirus, the ebola ... | 1995 | 7773195 |
| update: management of patients with suspected viral hemorrhagic fever--united states. | in 1988, cdc published guidelines for managing patients with suspected viral hemorrhagic fever (vhf) (1). pending a comprehensive review of the 1988 guidelines, this notice provides interim recommendations that update the 1988 guidelines for healthcare settings in the united states. this update applies to four viruses that cause syndromes of vhf: lassa, marburg, ebola, and congo-crimean hemorrhagic fever viruses; although the risk and/or mode of nosocomial transmission differs for each of these ... | 1995 | 7783731 |
| the asialoglycoprotein receptor is a potential liver-specific receptor for marburg virus. | the liver is one of the main target organs of marburg virus (mbg), a filovirus causing severe haemorrhagic fever with a high fatality rate in humans and non-human primates. mbg grown in certain cells does not contain neuraminic acid, but has terminal galactose on its surface glycoprotein. this observation indicated that the asialoglycoprotein receptor (asgp-r) of hepatocytes may serve as a receptor for mbg in the liver. binding studies revealed that the attachment of mbg to asgp-r-expressing hep ... | 1995 | 7844558 |
| [a case of a laboratory infection with marburg fever]. | 1994 | 7941853 | |
| [research with the marburg, lassa and ebola viruses]. | 1993 | 8059520 | |
| [the penetration of the marburg virus into eukaryotic cells]. | the early stages of infection of vero-e6 cell culture with marburg virus, a member of filovirus family, highly pathogenic for man, were studied. virus multiplication was completely or significantly inhibited by lysosomotropic agents (lta) of two types: weak base (ammonium chloride) and ionophore monensin. the level of the inhibiting effect was proportional to lta concentration and was maximal when the drug was introduced into the culture medium before virus inoculation. complete inhibition of ma ... | 1993 | 8059526 |
| [a comparative evaluation of an immunoenzyme analytical method using a fluorogenic or chromogenic substrate in determining the marburg virus antigen]. | a comparative evaluation of the developed variants of indirect solid-phase enzyme immunoassay using fluorogenic and chromogenic substrates for the determination of marburg virus antigen was carried out. the resolving capacity of this method was 3.8 x 10(-9) g of protein for the former and 3.1 x 10(-8) g of protein for the latter substrate. cross titrations demonstrated the lack of common antigens with ebola virus. | 1993 | 8073738 |
| the nucleoprotein of marburg virus is phosphorylated. | the nucleoprotein (np) of marburg virus (mbg), a filovirus, is encoded by the gene closest to the 3' end of the non-segmented negative-strand rna genome. sequence comparison has indicated that np is the functional equivalent to the nucleoproteins of paramyxoviruses and rhabdoviruses. expression of recombinant np in two eukaryotic systems using vaccinia virus and baculovirus (vectors psc11 and pacymb1, respectively) and analysis of mbg-specific proteins have demonstrated that the np of mbg is pho ... | 1994 | 8151297 |
| [the immunity indices of animals immunized with the inactivated marburg virus after infection with homologous virus]. | the data on changes in the parameters of specific and nonspecific immunity after challenge of the immunized animals with marburg virus are presented. mediators of the immune response: tumor necrosis factor, interferon, were shown to play different roles in the development of the disease and "protection" of animals. the survival rate of the immunized animals after the challenge with marburg virus was determined by the intensity and level of the immune response after challenge rather than by the l ... | 1994 | 8160440 |
| sequence analysis of the ebola virus genome: organization, genetic elements, and comparison with the genome of marburg virus. | sequence analysis of the second through the sixth genes of the ebola virus (ebo) genome indicates that it is organized similarly to rhabdoviruses and paramyxoviruses and is virtually the same as marburg virus (mbg). in vitro translation experiments and predicted amino acid sequence comparisons showed that the order of the ebo genes is: 3'-np-vp35-vp40-gp-vp30-vp24-l. the transcriptional start and stop (polyadenylation) signals are conserved and all contain the sequence 3'-uaauu. three base inter ... | 1993 | 8237108 |
| filovirus activity among selected ethnic groups inhabiting the tropical forest of equatorial africa. | seroepidemiological surveys were conducted to determine the frequency and distribution of filovirus activity among selected ethnic groups inhabiting the tropical forests of the central african republic. 427 serum specimens were collected from hunter-gatherers and subsistence farmers living in forest environs in the lobaye district south of the river lobaye and west of the river oubangui. striking serological evidence for filovirus activity was found in both populations. ebola virus appears to be ... | 1993 | 8266403 |
| marburg virus gene 4 encodes the virion membrane protein, a type i transmembrane glycoprotein. | gene 4 of marburg virus, strain musoke, was subjected to nucleotide sequence analysis. it is 2,844 nucleotides long and extends from genome position 5821 to position 8665 (embl data library, emnew: mvrepcyc [accession no. z12132]). the gene is flanked by transcriptional signal sequences (start signal, 3'-uacuucuuguaauu-5'; termination signal, 3'-uaauucuuuuu-5') which are conserved in all marburg virus genes. the major open reading frame encodes a polypeptide of 681 amino acids (m(r), 74,797). af ... | 1993 | 8437211 |
| replication of marburg virus in human endothelial cells. a possible mechanism for the development of viral hemorrhagic disease. | marburg and ebola virus, members of the family filoviridae, cause a severe hemorrhagic disease in humans and primates. the disease is characterized as a pantropic virus infection often resulting in a fulminating shock associated with hemorrhage, and death. all known histological and pathophysiological parameters of the disease are not sufficient to explain the devastating symptoms. previous studies suggested a nonspecific destruction of the endothelium as a possible mechanism. concerning the imp ... | 1993 | 8473483 |
| the vp35 and vp40 proteins of filoviruses. homology between marburg and ebola viruses. | the fragments of genomic rna sequences of marburg (mbg) and ebola (ebo) viruses are reported. these fragments were found to encode the vp35 and vp40 proteins. the canonic sequences were revealed before and after each open reading frame. it is suggested that these sequences are mrna extremities and at the same time the regulatory elements for mrna transcription. homology between the mbg and ebo proteins was discovered. | 1993 | 8482365 |
| the gp-protein of marburg virus contains the region similar to the 'immunosuppressive domain' of oncogenic retrovirus p15e proteins. | cdna was synthesized and cloned on the template of the genomic rna of marburg virus (strain popp). recombinant plasmids with specific cdna inserts were selected and sequenced. the length of the open reading frame encoding the gp-protein is 681 amino acids. gp-protein is proposed to be an integral membrane protein. computer-assisted comparison of the deduced amino acid sequence with those of different viruses revealed significant homology with the gp-protein of ebola virus and with the 'immunosup ... | 1993 | 8495737 |
| [inhibition of marburg virus reproduction by glycyrrhizinic acid and its derivatives]. | 1995 | 8563649 | |
| filovirus-induced endothelial leakage triggered by infected monocytes/macrophages. | the pathogenetic mechanisms underlying hemorrhagic fevers are not fully understood, but hemorrhage, activation of coagulation, and shock suggest vascular instability. here, we demonstrate that marburg virus (mbg), a filovirus causing a severe form of hemorrhagic fever in humans, replicates in human monocytes/macrophages, resulting in cytolytic infection and release of infectious virus particles. replication also led to intracellular budding and accumulation of viral particles in vacuoles, thus p ... | 1996 | 8642644 |
| [survival of marburg virus infectivity on contaminated surfaces and in aerosols]. | marburg virus was shown to survive for up to 4-5 days on contaminated surfaces. in aerosol it was not stable, the specific rate of its inactivation being 0.05 min-1. this brought the authors to a conclusion that a relatively close contact is needed for virus transmission from man to man, although the possibility of aerosol transmission of the infection may be appreciably increased in case of the hemorrhagic syndrome with a high level of viremia. | 1996 | 8669144 |
| [disinfecting action of chloramine b on marburg virus]. | 1996 | 8669148 |